scholarly journals Ameliorative efficacy of Sepia officinalis ink extract on hepatorenal injury-induced following high-dose folic acid supplementation in rats

2021 ◽  
Vol 17 (3) ◽  
pp. 038-046
Author(s):  
Mariam T Elrouby ◽  
Sara B Ali ◽  
Nagat Saleh Hossuin Moftha ◽  
Abdeljalil Mohamed Al Shawoush ◽  
Ayman S. Mohamed
Keyword(s):  
Kidney Injury ◽  
Folic Acid Supplementation ◽  
Total Serum ◽  
Albino Rats ◽  
High Dose ◽  
Sepia Officinalis ◽  
High Intake ◽  
Stress Markers ◽  
Ameliorative Effect ◽  
Serum Aminotransferases

Sepia ink, a black suspension of melanin granules, is a multifunctional marine bioactive material. The present study aims to evaluate the ameliorative effect of the ink extract (IE) of the cuttlefish (Sepia officinalis) during high dosage administration of the FA in rats. Kidney injury induced by a single oral dose of FA (250 mg /kg). Eighteen male Wistar albino rats were the control, FA group, and FA+ IE group (250mg/kg). The IE showed a significant ameliorative effect against hepatorenal injury induced by high intake of FA as evident by decreasing the levels of serum aminotransferases (AST and ALT), urea, creatinine, uric acid, and significantly increased total serum albumin. Treatment with IE normalized the antioxidant status of the injured animals by reducing the MDA and the significant increase in the levels of GSH and CAT. The present study revealed that IE had an insightful effect against hepatorenal injury-induced following high intake of FA in rats, as it alleviates the alterations in the oxidative stress markers.

scholarly journals Hepatoprotective Activity of Melittin on Isoniazid and Rifampicin Induced Liver Damage in Male Albino Rats

2020 ◽  
Author(s):  
Khalid M. Naji ◽  
Bushra Y. Al-Khatib ◽  
Nora Saif Al-Haj ◽  
Myrene R. D’souza
Keyword(s):  
Acute Hepatic Failure ◽  
Hepatoprotective Activity ◽  
Total Serum Protein ◽  
Total Serum ◽  
Albino Rats ◽  
Antitubercular Drug ◽  
Drug Induced ◽  
Ameliorative Effect ◽  
Histopathological Studies ◽  
Hematological Alterations

AbstractObjectiveThe present study aimed to investigate the ameliorative effect of melittin, a major polypeptide in the venom of honeybee (Apis mellifera) on isoniazid (INH) and rifampicin (RIF) induced hepatotoxicity in male albino rats. Method: The rats (140-200g) were divided into five groups (n=6): normal control (NC); toxic (T) group treated with INH+RIF (100 mg/kg, p.o.); melittin-treated (Mel15, Mel30) group (15 or 30 µg/kg s.c); and normal recovery (NR) group. Blood and liver samples were collected for biochemical, hematological and histopathological studies respectively.ResultsThe administration of melittin was found to prevent the antitubercular drug-induced alterations in the diagnostic markers; reduced glutathione (GSH), direct bilirubin (DB), total bilirubin (TB), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and total serum protein (TSP). Besides, hematological alterations were significantly high (P<0.0001) in Mel-treated groups when compared to the toxic control. The NR group exhibited lower levels of DB, TB, ALP, LDH and TSP. In addition, treatment with melittin offered protection in the NR group with respect to MDA levels.ConclusionEvidence from this study indicate that melittin is beneficial for the prevention of acute hepatic failure in antitubercular drug-induced hepatoxicity.

scholarly journals Nephroprotective Activity of Ethanolic Extract of Carissa carandas Leaves against Gentamicin-Induced Acute Kidney Injury in Wistar Albino Rats

2021 ◽  
pp. 279-286
Author(s):  
V. V. Rajesham ◽  
Hima Sowmya Kandoti ◽  
Mitta Raghavendra ◽  
P. Roshan Ali ◽  
T. Rama Rao
Keyword(s):  
Kidney Injury ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Oxidative Stress Markers ◽  
Disease Group ◽  
Stress Markers ◽  
Carissa Carandas ◽  
Nephroprotective Activity ◽  
Wistar Albino Rats ◽  
Histopathological Studies

The present study was aimed to evaluate the nephroprotective activity of ethanolic extract of Carissa carandas Linn. Leaves (EECC) in Gentamicin-induced nephrotoxicity in Wistar albino rats. The renal damage was induced by Gentamicin (80mg/kg body weight, i.p.). Nephroprotective activity was investigated by the administration of EECC at two different doses (100 and 200mg/kg body weight, p.o) for 28 days and by assessing serum parameters, renal oxidative stress markers and histopathological studies. Gentamicin-treated animals showed an increase in serum creatinine, uric acid, urea, and malondialdehyde (MDA) levels and decrease in total protein, reduced glutathione (GSH), and catalase(CAT) compared to normal control animals, which indicates severe nephrotoxicity. Histopathological studies of kidney Gentamicin-treated animals showed extensive acute tubular necrosis and peri-tubular inflammation. Administration of EECC showed a significant improvement (p<0.05) in biochemical and oxidative stress markers compared to the disease group. EECC treated groups showed better histological appearance when compared to the disease group. Ethanolic extract of Carissa carandas Linn. Leaves showed significant nephroprotective activity against gentamicin-induced acute kidney injury.

scholarly journals Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model

2021 ◽  
Vol 22 (6) ◽  
pp. 3044
Author(s):  
Noha M. Abogresha ◽  
Sally S. Mohammed ◽  
Marwa M. Hosny ◽  
Hoda Y. Abdallah ◽  
Ahmed M. Gadallah ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Target Genes ◽  
Histopathological Examination ◽  
Protective Role ◽  
Premature Ovarian Insufficiency ◽  
Albino Rats ◽  
High Dose ◽  
Ovarian Insufficiency ◽  
Stress Markers ◽  
Intraperitoneal Dose

The current study was designed to investigate the protective role of diosmin against cyclophosphamide-induced premature ovarian insufficiency (POI). Female Swiss albino rats received a single intraperitoneal dose of cyclophosphamide (200 mg/kg) followed by 8 mg/kg/day for the next 15 consecutive days either alone or in combination with oral diosmin at 50 or 100 mg/kg. Histopathological examination of ovarian tissues, hormonal assays for follicle stimulating hormone (FSH), estradiol (E2), and anti-Mullerian hormone (AMH), assessment of the oxidative stress status, as well as measurement of the relative expression of miRNA-145 and its target genes [vascular endothelial growth factor B (VEGF-B) and regulator of cell cycle (RGC32)] were performed. Diosmin treatment ameliorated the levels of E2, AMH, and oxidative stress markers. Additionally, both low and high diosmin doses significantly reduced the histopathological alterations and nearly preserved the normal ovarian reserve. MiRNA-145 expression was upregulated after treatment with diosmin high dose. miRNA-145 target genes were over-expressed after both low and high diosmin administration. Based on our findings, diosmin has a dose-dependent protective effect against cyclophosphamide-induced ovarian toxicity in rats.

scholarly journals Effect of Tamarindus indica juice intake on some oxidative stress markers in carbon tetrachloride induced rats

2021 ◽  
Vol 14 (1) ◽  
pp. 31-37
Author(s):  
N.S. Sadi ◽  
S.M. Abubakar ◽  
A. Ibrahim ◽  
A.M. Umar ◽  
A.M. Gadanya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Normal Control ◽  
Colorimetric Assay ◽  
Normal Control Group ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Oxidative Stress Markers ◽  
Standard Drug ◽  
Stress Markers

Tamarind tree is a multipurpose tree of which almost every part finds at least some use, either nutritional or medicinal. Due to its pleasant acidic taste and rich aroma, the pulp is widely used for domestic and industrial purpose. A study was carried out to evaluate the effect of Tamarind juice intake in CCl4 induced oxidative stress albino rats. The Proximate, antinutrient, and Phytochemical contents of tamarind juice were analyzed using standard AOAC methods while mineral contents were determined using atomic absorption spectrometry. Oxidative stress markers were also analyzed using colorimetric assay kit. The serum levels of oxidative stress markers were compared between the normal and test groups. Experimental rats were divided into five groups: Normal control group, negative control (CCl4) group, standard drug (Vitamin C) group, tamarind low and high dose group. At the end of the experiment, significant increase in malondialdehyde level and decrease in superoxide dismutase, catalase, reduced glutathione and glutathione Peroxidase activities were recorded in CCl4-exposed rats as compared to normal control group. In the tamarind supplemented groups, the level of MDA along with the activities of SOD, CAT, GSH and GPx were comparable with the normal control rats (p>0.05). Thus, it appears that tamarind juice ameliorate the effect of CCl4; suggesting that consumption of natural compounds with an antioxidant profile may be a preventive alternative to those diseases associated with oxidative stress.

scholarly journals Hepatoprotective activity of melittin on isoniazid- and rifampicin-induced liver injuries in male albino rats

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Khalid Mohammed Naji ◽  
Bushra Yahya Al-Khatib ◽  
Nora Saif Al-Haj ◽  
Myrene R. D’souza
Keyword(s):  
Acute Hepatic Failure ◽  
Hepatoprotective Activity ◽  
Total Serum Protein ◽  
Total Serum ◽  
Albino Rats ◽  
Antitubercular Drug ◽  
Drug Induced ◽  
Liver Injuries ◽  
Ameliorative Effect ◽  
Histopathological Studies

Abstract Background The present study investigated the ameliorative effect of melittin, a major polypeptide in the venom of honeybee (Apis mellifera), on isoniazid-(INH) and rifampicin-(RIF) induced hepatotoxicity in male albino rats. Method Thirty rats (140-200 g) were divided into five groups (n = 6): normal control (NC) received normal saline orally (NaCl, 0.9%; toxic (T) group received INH + RIF (each rat received 100 mg/kg, p.o.); melittin (Mel15, Mel30) groups (each rat received 15 or 30 μg/kg s.c); and normal recovery (NR) group received INH + RIF (each rat received 100 mg/kg, p.o.). Blood and liver samples were collected for biochemical, hematological and histopathological studies respectively. Results The administration of melittin was found to prevent the antitubercular drug-induced alterations in the diagnostic markers; reduced glutathione (GSH), direct bilirubin (DB), total bilirubin (TB), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and total serum protein (TSP). Besides, hematological alterations were significantly high in Mel groups when compared to the toxic group. The NR group exhibited lower levels of DB, TB, ALP, LDH and TSP. In addition, treatment with melittin offered protection in the NR group with respect to MDA levels. Conclusion Evidence from this study suggests that melittin is beneficial for the prevention of acute hepatic failure in antitubercular drug-induced hepatoxicity and could be used as a potential therapeutic agent.

scholarly journals Ameliorative effect of hydroalcoholic extracts of Nigella sativa seed against CCl4-induced acute liver injury in rats

2020 ◽  
Vol 10 (3) ◽  
pp. 164-169 ◽  
Author(s):  
Ikenna Kingsley Uchendu
Keyword(s):  
Carbon Tetrachloride ◽  
Low Dose ◽  
Nigella Sativa ◽  
Control Group ◽  
Albino Rats ◽  
Average Weight ◽  
High Dose ◽  
Histopathological Analysis ◽  
Hydroalcoholic Extract ◽  
Ameliorative Effect

Objective:  The aim of this study is to investigate the ameliorative effect of hydroalcoholic extract of Nigella sativa (HANS) against CCl4-induced hepatotoxicity in albino rats. Methods: Twenty five (25) albino rats, with average weight (105±5g), were randomly grouped into five groups: A-E, of five (5) rats per group. Group A rats served as normal control, Group B (Negative Control) received intraperitoneal administration of carbon tetrachloride CCl₄ (0.4ml/kg, i.p.) only, Group C received CCl₄ and low dose HANS (400mg/kg, oral), Group D received CCl₄ and high dose HANS (800mg/kg, oral), and Group E (Positive control), received CCl₄ and Vitamin C (200mg/kg, oral), for 3 days. Hepatotoxicity was assessed by measuring serum levels of total bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) using standard methods.  Histopathological analysis of the liver was also carried out. Results: The extracts significantly stablized biochemical markers of hepatic injury, and preserved the histoarchitecture of the liver tissues against CCl4 damage. The protective effect was not dose-dependent, as low dose HANS (400mg/kg), showed better protection than the high dose HANS (800mg/kg). Conclusion: Hydroalcoholic extracts of Nigella sativa has antihepatotoxic effects. Keywords: carbon tetrachloride, hepatotoxicity, medicinal plants, Nigella sativa, hydroalcoholic extract

scholarly journals ANALYSIS OF OXIDATIVE STRESS MARKERS IN CHRONIC CONSUMPTION OF MONOSODIUM GLUTAMATE ON LIVER OF WISTAR ALBINO RATS

2021 ◽  
pp. 116-119
Author(s):  
SURENDRA BABU THANGACHI ◽  
VARSHA SRIRAM MOKHASI ◽  
AGA AMMAR MURTHUZA
Keyword(s):  
Oxidative Stress ◽  
Monosodium Glutamate ◽  
Liver Homogenate ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Wistar Albino Rats

Objective: The study was intended to explore whether Monosodium glutamate (MSG) induces oxidative stress on the liver of Wistar albino rats when fed chronically at three different doses, namely, low, mid, and high doses identical to human consumption doses in growing countries. Methods: The acclimatized Wistar albino rats (n=24) were randomly selected and grouped into four groups, namely Control, Low dose MSG (180 mg kg), Mid dose MSG (360 mg/kg), and High dose MSG (720 mg/kg). The animals were orally administered MSG for 120 days. After completion of the experimental period (120 days), euthanized animal liver was homogenized to investigate the oxidative stress marker enzymes such as Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Catalase (CAT), and Myeloperoxidase (MPO). Results: The MPO showed a significant increase (p<0.05) in liver homogenate of all MSG induced groups when compared to control group. The SOD, CAT, and GPx activity deteriorated (p<0.05) in monosodium induced groups contrasting to the control group. Conclusion: The effects of MSG on oxidative stress markers on liver homogenate in the current study exhibited erratic abnormal changes in oxidative stress markers of monosodium induced groups which contemplate the harmful effects of MSG consumed chronically. The further studies should confirm the genetic basis of oxidative stress damage and transform the safety regulations of MSG consumption throughout the world.

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