scholarly journals Antimicrobial activity of Punica grantum leaves

2021 ◽  
Vol 3 (2) ◽  
pp. 214-217
Author(s):  
Thammanna Gowda SS ◽  
Parimala B Hanumesh ◽  
Manjula RV
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Ethanol ◽  
Total Phenolics ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Human Beings ◽  
Disc Diffusion Method ◽  
Contagious Diseases ◽  
Varied Concentration

Natural phytochemicals isolated from medicinal plants acts as inhibitors for pathogenic microorganisms which causes contagious diseases to human beings. The present study was done to explore the antimicrobial activity of aqueous and aqueous-ethanol extract of Punica grantum leaves. The phytochemical analysis of both the extracts revealed the presence of total phenolics & flavonoids at varied concentration. The antimicrobial activity of the extracts was carried out by disc diffusion method. Both the aqueous and aqueous-ethanol extract showed the antimicrobial activity against S. aureus, Shi. dysentriae & Shi. flexineri. The antimicrobial activity of the Punica grantum leaves extracts could be attributed by the presence of phenolics and flavonoids.

scholarly journals Activity Of Lingzhi Fungan Ethanol Extract (Ganoderma Lucidum) Using Soxhlet Method For Bacteria Propionibacterium Acnes

2020 ◽  
Vol 12 (1) ◽  
pp. 151
Author(s):  
Prasetyo Handrianto
Keyword(s):  
Antimicrobial Activity ◽  
Ganoderma Lucidum ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Research Data ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Inhibitory Zone ◽  
Disc Diffusion Method ◽  
Soxhlet Method

The purpose of this study is to know Lingzhi Mushroom extract (Ganoderma Lucidum) has antimicrobial activity against Propionibacterium acnes using Ethanol Solvent, the method used is the disc diffusion method using 6 repetitions, the concentration used is 20 µg / ml, 40 µg / ml, 60 µg / ml / ml, 80 µg / ml and 100 µg / ml, Lingzhi mushroom was extracted using ethanol solvent with the Soxhlet method. The results of the research data showed that the largest inhibitory zone was shown at a concentration of 100 µg / ml of 6.91 mm with a medium inhibitory category. The conclusion of this research is the Lingzhi mushroom is able to inhibit the activity of Propionibacterium acnes in the medium category.

The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

2019 ◽  
Vol 18 (5) ◽  
pp. 262-274
Author(s):  
E. Benyagoub ◽  
N. Nabbou ◽  
S. Boukhalkhel ◽  
I. Dehini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Fusidic Acid ◽  
Quercus Robur ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals A comparative evaluation of Kumaun Himalayan Gymnosperms for their Antifungal potential against plant pathogenic fungi

2018 ◽  
Vol 7 (3) ◽  
pp. 230-241
Author(s):  
Savita Joshi ◽  
◽  
Parikshit Kumar ◽  
Prabha Pant ◽  
SC Sati ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Total Activity ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Cedrus Deodara ◽  
Araucaria Cunninghamii

Fungicidal activity of 10 ethnobotanically known Kumaun Himalayan gymnospermous plants namely Araucaria cunninghamii, Biota orientalis, Cedrus deodara, Cephalotaxus griffithi, Cryptomeria japonica Cupressus torulosa, Ginkgo biloba, Juniperus communis, Picea smithiana and Pinus wallichiana were tested against six plant disease causing fungal pathogens by agar well-diffusion method. Forty extracts of these gymnospermic leaves in different organic solvents (methanol, ethanol, chloroform and hexane) were studied by performing the 160 sets of experiments. The MIC values of each extract (where % inhibition ≥ 40%) were also determined. All the plant extracts exhibited strong antifungal activity. Results indicated that all leaves extracts of C. griffithi and G. biloba were found most effective among the tested plants extracts. Hexane extract of C. griffithi was showed highest inhibitory activity against C. falcatum (72%; MIC, 7.81µg/ml) and T. indica (70%; MIC, 15.62µg/ml). On the other hand, ethanol extract of G. biloba also showed remarkable activity against P. oryzae (66% with MIC, 7.81g/ml). While P. wallichiana leave extracts were found less active among the studied plants against all the tested fungal strains. The chloroform extracts were found the most effective against all the tested fungi (10% to 60%), followed by ethanol extract (30-50%), methanol extract (20-40%), while in hexane extracts ranged 10-30% only. The extracts of C. griffithi exhibited superior Relative Antifungal Activity (RAA, 20%), followed by G. biloba and A. cunninghamii (RAA, 19 and 12%, respectively). All data were also analyzed for determination of total activity of plant for each studied species of gymnosperm. C. griffithi had maximum activity i.e. 71 % followed by G. biloba (54%) and A. cunninghamii (33%). C. torulosa showed the least total activity and RAA i.e. 8% and 3%, respectively. All the plant species assayed possess definite antifungal properties and suggested for phytochemical analysis to identify the active principles responsible for their antifungal activity

scholarly journals Antimicrobial and larvicidal potential of endophytic fungi isolated from Tragia involucrata Linn.

2016 ◽  
Vol 6 (01) ◽  
pp. 1494 ◽  
Author(s):  
Ananda Danagoudar ◽  
Chandrashekhar G. Joshi* ◽  
M.T. Nivya ◽  
H.M. Manjunath ◽  
Jagadeesha Poyya ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Penicillium Citrinum ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
The People ◽  
Therapeutic Compounds

Endophytic fungi are one of the untapped resources of therapeutic compounds for various diseases. The present study focused on the antimicrobial as well as larvicidal activity of ethyl acetate extract of endophytic fungi isolated from Tragia involucrata. The ethyl acetate extract of Penicillium citrinum CGJ-C1 (GenBank No.KT780618), Penicillium citrinum CGJ-C2 (KP739821), Cladosporium sp. (KP739822), and Cryptendoxyla hypophloia CGJ-D2 (KT780619) was subjected to antimicrobial activity against a panel of microorganisms by disc diffusion method, larvicidal activity against Culex quinquefasciatus. All the extracts showed significant antimicrobial activity against the tested organisms ranging from 8±0.32 to 13±2.11. The extent of activity was comparable to the standard drugs. The larvicidal potential of the endophytes was superior to T.involucrata extract. The larvicidal activity was found to be dose and time dependent with LC50 value ranging from 4.25- 158.06ppm after 24hrs of treatment. This is the first report on the bioactivity of the endophytes isolated from T.involucrata. Further studies on the bio-guided isolation of lead compound will benefit the people suffering from microbe’s related diseases.

scholarly journals Antibacterial Activity of Ethanol Extract of Spirulina platensis

2021 ◽  
Vol 7 (2) ◽  
pp. 47-51
Author(s):  
Prayoga Pannindrya ◽  
Mega Safithri ◽  
Kustiariyah Tarman
Keyword(s):  
Antibacterial Activity ◽  
Spirulina Platensis ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Test Results ◽  
Nutritional Content ◽  
Disc Diffusion Method ◽  
Antibacterial Test ◽  
E Coli

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.

scholarly journals Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

2019 ◽  
Vol 31 (5) ◽  
pp. 1077-1080
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
V. Ravi Sankar Ummdi ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Triazole Ring ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antibacterial Study ◽  
Gram Negative ◽  
E Coli ◽  
Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

scholarly journals ANTIFUNGAL ACTIVITY OF PIMENTA DIOICA (L.) MERRIL AN AROMATIC MEDICINAL TREE

2016 ◽  
Vol 8 (10) ◽  
pp. 92 ◽  
Author(s):  
Akhilesh Bhat ◽  
Raveesha K. A.
Keyword(s):  
Antifungal Activity ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microsporum Canis ◽  
Ether Extract ◽  
Disc Diffusion Method ◽  
Rf Values ◽  
Pimenta Dioica

<p><strong>Objective</strong>:<strong> </strong>To assess the antifungal activities of the extracts of leaves of <em>Pimenta dioica</em> against human pathogenic fungi and identify the class of phytochemical responsible for the biological activity.</p><p><strong>Methods</strong>:<strong> </strong>The shade dried, and powdered leaves of <em>Pimenta dioica</em> were subjected to polarity based solvent extraction by soaking. The extracts were dried in a rotary flash evaporator and subjected to antifungal activity assay against <em>Candida albicans</em>, <em>Microsporum canis </em>and<em> M. gypseum</em>, by disc diffusion method. Antifungal active petroleum ether extract was subjected to phytochemical analysis to identify the active compound. The extract was then subjected to Thin Layer Chromatography (TLC) and Bioautography, by agar overlay method. The fractions were collected by Preparative TLC method and subjected to antifungal activity assay by disc diffusion method. Further, the Libermann-Burchard test was conducted to detect the broad group of compounds responsible for the bioactivity.</p><p><strong>Results</strong>:<strong> </strong>Among the five extracts viz. petroleum ether, chloroform, ethyl acetate, methanol and aqueous, the petroleum ether extract showed significant antifungal activity against <em>Microsporum canis</em> (50 mm), <em>Microsporum gypseum</em> (60 mm) and <em>Candida albicans</em> (23 mm). Terpenoids, tannins and flavonoids were found to be the chief constituents of the petroleum ether extract. The TLC of the extract revealed 7 spots with the Rf values 0.37, 0.42, 0.45, 0.52, 0.59, 0.76, 0.96 with the solvent system of hexane and acetone (1:0.5). The bioautography assay revealed inhibitory activity in the spots at Rf values 0.52 and 0.59. These fractions obtained by preparative TLC also confirmed the same. The phytochemical analysis of the fractions on the TLC plate suggests that the compound is triterpene or steroid.</p><p><strong>Conclusion</strong>:<strong> </strong>The antifungal activity of <em>Pimenta dioica</em> and the bioactive phytochemical has been reported in the present study. Preliminary phytochemical analysis has indicated that the compounds are triterpenes or steroids. The results indicate that the active compounds are potential lead molecules for new antifungal drugs in the management of infectious fungi.</p>

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

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