scholarly journals Antibacterial effect of polyphenols enriched drumstick plant leaves (Moringa oleifera) extract: A research study

2020 ◽  
Vol 8 (2) ◽  
pp. 158-163
Author(s):  
Vedamurthy Joshi ◽  
Rajesh Kowti ◽  
B. Ramesh ◽  
Santhosh Kumar Nune ◽  
Dinesha Ramadas
Keyword(s):  
Staphylococcus Aureus ◽  
Moringa Oleifera ◽  
Antimicrobial Agents ◽  
Research Study ◽  
Antibacterial Effect ◽  
Culture Collection ◽  
Bacterial Activity ◽  
Plant Leaves ◽  
Bacterial Type ◽  
Bacterial Effect

Microorganisms are raising resistance against available antibiotics due to usage of antibiotics in a wrong way. There is lot of demand for finding /searching new natural antimicrobial agents as they are inexpensive, easily available, most of them are edible and mainly non-toxic even at the rate of milligram quantity. The aim was to study the efficiency of the anti-bacterial effect of Polyphenols enriched extract of Drum stick plant leaves (Moringa oleifera) against staphylococcus aureus. The materials involved in this study include Drum stick plant leaves, micro-organism staphylococcus aureus in the bacterial type culture collection, agar, and blood-agar plates. At 10% concentration, of Polyphenols enriched extract of Drum stick leaves had zero anti-bacterial activity, while between 20 to 25% concentrations revealed high activity against the bacteria. Thus, increased in the anti-bacterial activity was promising as the concentration augmented from 20 to 25%. The results acquired from this study points that polyphenol enriched extract of Drum stick leaves (Moringa oleifera) had antibacterial property against Staphylococcus aureus when obtained to a necessary concentration.

scholarly journals Antimicrobial Potential of Sicilian Honeys Against Staphylococcus aureus and Pseudomonas aeruginosa

2018 ◽  
Vol 101 (4) ◽  
pp. 956-959 ◽  
Author(s):  
Maria Anna Coniglio ◽  
Pasqualina Laganà ◽  
Giuseppina Faro ◽  
Marina Marranzano
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Culture Collection ◽  
Significant Inhibition ◽  
P Value ◽  
Chi Square ◽  
Diffusion Assay

Abstract The purpose of this study was to investigate the antibacterial effect of 71 organic Sicilian honeys of different botanical origins against Staphylococcus aureus [American Type Culture Collection (ATCC) 9144™] and Pseudomonas aeruginosa (ATCC 27853™). The antimicrobial activity was determined by means of an agar diffusion assay from the estimation of the diameter of the inhibition zone produced by the honeys. S. aureus was more inhibited than P. aeruginosa (chi-square value 11.2698, P value 0.000788). In particular, a statistically significant inhibition growth against S. aureus was exhibited by the polyfloral (chi-square value 6.1714, P value 0.012983) and the eucalyptus honeys (chi-square value 4, P value 0.0455). Results partially suggest Sicilian organic polyfloral and eucalyptus honeys as possible alternatives to antimicrobial agents when S. aureus resistance causes failure of initial conventional antibiotic treatment.

Anti-Bacterial from Moringa oleifera Lamk Crude Extracts

2014 ◽  
Vol 1060 ◽  
pp. 223-226
Author(s):  
Perayot Pamonsinlapatham
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Moringa Oleifera ◽  
Soxhlet Extraction ◽  
Stem Bark ◽  
Bacterial Activity ◽  
Diffusion Method ◽  
Crude Extracts ◽  
Agar Diffusion Method

Moringa oleiferaLamk (Moringaceae) is a worldwide use for food and traditional medicine. In this study, plants were separated into seven parts: 1) Leaf, 2) Drum, 3) Branching, 4) Seed, 5) Outer stem, 6) Stem bark, and 7) Root, respectively. Then, they were extracted by two different methods: methanol soxhlet extraction and ethanol maceration. All crude extracts were tested for anti-bacterial activity by agar diffusion method and colorimetric micro-dilution plate technique. The results showed that 7 parts of Moringa crude extracts had low anti-bacterial activity againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawhen compared with standard antibiotic (ampicillin or gentamycin). In conclusion, anti-bacterial effects ofM. oleiferacrude extracts had low anti-bacterial activityin vitrotest.

IDENTIFICATION OF CHEMICAL COMPOUNDS AND ANTIBACTERIAL ACTIVITY OF 96% ETHANOL EXTRACT FROM MORINGA OLEIFERA LAM. LEAVES AGAINST MRSA (METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS)

2021 ◽  
pp. 111-114
Author(s):  
NURSANNA IRAWATY SINAGA ◽  
MUHAMMAD HANAFI ◽  
NOVI YANTIH
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Methicillin Resistant ◽  
Activity Test ◽  
Moringa Oleifera Lam

Objective: Infection with antibiotic-resistant organisms, requiring the selection of the right drug to fight these organisms. Moringa oleifera Lam. leaves as natural ingredients have MRSA (Methicillin-Resistant Staphylococcus aureus) antibacterial activity. The purpose of this study is to identify the chemical compounds and to determine the antibacterial activity from the 96% ethanol extract of Moringa oleifera Lam. leaves against MRSA bacteria. Methods: The Moringa oleifera Lam. leaves were extracted by maceration using 96% (v/v) ethanol. Mass spectrometry was performed on an LC-MS/MS Xevo, G2-XS QTof (Waters MS Technologies) to identified chemical compounds from the extract. The ionization type is ESI. The method of the antibacterial activity test was using agar paper disc diffusion. Antibacterial activity was based on the diameter of the bacterial inhibition zone. Results: The result of the antibacterial activity test for 96% ethanol extract of Moringa oleifera Lam. leaves a concentration of 10, 20, and 40 % each has inhibition diameter was 10, 13, and 15.5 mm, and for linezolid as a positive control at 30µg has diameter inhibition was 20 mm, ethanol 96% as a negative control was 0 mm. The 96% ethanol extract of Moringa oleifera Lam. leaves contains 13-hydroxy-9,11-hexadecadienoic acid, 3-tert-butyl-4-methoxyphenol, bistortaside, daturametelin H, digiprolactone, ephedradine C, kaempferol-3-O-rutinoside,kaempferol-3-O-β-D-glucopyranoside, kaempferol-7-O-α-L rhamnoside, phenyl propionic acid, pyrophaeophorbide A, quercetin, stearidonic acid, stigmastan-3,6-dione. Conclusion: The 96% ethanol extract of Moringa oleifera Lam. leaves contains 14 compounds. The 96% ethanol extract of Moringa oleifera Lam. leaves have activity against MRSA bacteria. The antibacterial effect of the extract increased with an increase in its concentration. The extract exerted a greater antibacterial effect on the concentration 40%.

scholarly journals Perbedaan Efek Antibakteri Kapsul Minyak Bawang Putih (Garlic Oil) dan Kapsul Bubuk Bawang Putih (Garlic Powder) Terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli Secara In Vitro

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Ismail Fajri ◽  
Erly Erly ◽  
Elly Usman
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Alternative Therapy ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Garlic Powder ◽  
Garlic Oil ◽  
Bacterial Effect

Abstrak   Bawang putih  telah diekstrak berupa kapsul garlic oil dan kapsul garlic powder sehingga dapat digunakan sebagai alternatif terapi dalam pengobatan penyakit akibat infeksi bakteri. Kandungan kapsul bawang putih yang memiliki efek anti bakteri adalah allicin. Bawang putih mempunyai efek anti mikroba dalam melawan bakteri Gram positif dan Gram negatif, diantaranya Staphylococcus aureus dan Escherichia coli. Di pasaran terdapat dua jenis kapsul bawang putih, yaitu kapsul bubuk bawang putih dan kapsul minyak bawang putih.  Tujuan penelitian ini adalah menentukan perbedaan efek antara antibakteri kapsul minyak bawang putih dan kapsul bubuk bawang putih terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.  Penelitian ini dilakukan di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas Padang dari Mei sampai Juni 2014. Jenis penelitian adalah eksperimental dengan menggunakan metode difusi cakram. Hasil penelitian didapatkan kapsul minyak bawang putih dan kapsul bubuk bawang putih tidak memiliki kemampuan untuk menghasilkan zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Walaupun tiga kali pengulangan,  tidak terdapat efek antibakteri pada kapsul minyak bawang putih dan kapsul bubuk bawang putih.Kesimpulan penelitian ini adalah kapsul minyak bawang putih dan kapsul bubuk bawang putih tidak memiliki efek antibakteri terhadap Staphylococcus aureus dan Escherichia coli.Kata kunci: minyak bawang putih, kapsul bubuk bawang putih, Staphylococcus aureus, Escherichia coli AbstractGarlic extract in garlic oil capsules and garlic powder capsules can be used as alternative therapy in the treatment of diseases caused by bacterial infection. The content of garlic capsules which have anti-bacterial effect is allicin. Garlic has anti-microbial effect against Gram positive and Gram negative, including Staphylococcus aureus and Escherichia coli. In the market there are two types of garlic capsules, the capsules of garlic powder and garlic oil capsules. The objective of this study was to determine the differences of antibacterial effect between garlic oil capsules and garlic powder capsules on the growth of Staphylococcus aureus and Escherichia coli. This research was done in the laboratory of Microbiology, Faculty of Medicine, Andalas University, Padang in Mei to Juny 2014. The type of research is experimental by using the disc diffusion method. The results of this study, garlic oil capsules and garlic powder capsules do not have the ability to produce a zone of inhibition against Staphylococcus aureus and Escherichia coli. Although 3 (three) time  repetitions, there were no antibacterial effect from garlic oil capsules and garlic powder capsules. The conclusion of this study is garlic oil capsules and garlic powder capsules do not have antibacterial effects against Staphylococcus aureus and Escherichia coli.         Keywords: garlic oil capsule, garlic powder capsule, Staphylococcus aureus, Escherichia coli

Biological Evaluation of Selected 1,2,3-triazole Derivatives as Antibacterial and Antibiofilm Agents

2020 ◽  
Vol 20 (24) ◽  
pp. 2186-2191
Author(s):  
Lialyz Soares Pereira André ◽  
Renata Freire Alves Pereira ◽  
Felipe Ramos Pinheiro ◽  
Aislan Cristina Rheder Fagundes Pascoal ◽  
Vitor Francisco Ferreira ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Public Health Problem ◽  
Biological Evaluation ◽  
Major Public Health Problem ◽  
Community Environments ◽  
Scanning Electron

Background: Resistance to antimicrobial agents is a major public health problem, being Staphylococcus aureus prevalent in infections in hospital and community environments and, admittedly, related to biofilm formation in biotic and abiotic surfaces. Biofilms form a complex and structured community of microorganisms surrounded by an extracellular matrix adhering to each other and to a surface that gives them even more protection from and resistance against the action of antimicrobial agents, as well as against host defenses. Methods: Aiming to control and solve these problems, our study sought to evaluate the action of 1,2,3- triazoles against a Staphylococcus aureus isolate in planktonic and in the biofilm form, evaluating the activity of this triazole through Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests. We have also performed cytotoxic evaluation and Scanning Electron Microscopy (SEM) of the biofilms under the treatment of the compound. The 1,2,3-triazole DAN 49 showed bacteriostatic and bactericidal activity (MIC and MBC 128 μg/mL). In addition, its presence interfered with the biofilm formation stage (1/2 MIC, p <0.000001) and demonstrated an effect on young preformed biofilm (2 MICs, p <0.05). Results: Scanning Electron Microscopy images showed a reduction in the cell population and the appearance of deformations on the surface of some bacteria in the biofilm under treatment with the compound. Conclusion: Therefore, it was possible to conclude the promising anti-biofilm potential of 1,2,3-triazole, demonstrating the importance of the synthesis of new compounds with biological activity.

scholarly journals Pseudomonas aeruginosa PA14 Enhances the Efficacy of Norfloxacin against Staphylococcus aureus Newman Biofilms

2020 ◽  
Vol 202 (18) ◽  
Author(s):  
Giulia Orazi ◽  
Fabrice Jean-Pierre ◽  
George A. O’Toole
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Respiratory Infections ◽  
Multiple Infection ◽  
Bacterial Biofilms ◽  
Interspecies Interactions ◽  
Chronic Infections ◽  
Content Type

ABSTRACT The thick mucus within the airways of individuals with cystic fibrosis (CF) promotes frequent respiratory infections that are often polymicrobial. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most prevalent pathogens that cause CF pulmonary infections, and both are among the most common etiologic agents of chronic wound infections. Furthermore, the ability of P. aeruginosa and S. aureus to form biofilms promotes the establishment of chronic infections that are often difficult to eradicate using antimicrobial agents. In this study, we found that multiple LasR-regulated exoproducts of P. aeruginosa, including 2-heptyl-4-hydroxyquinoline N-oxide (HQNO), siderophores, phenazines, and rhamnolipids, likely contribute to the ability of P. aeruginosa PA14 to shift S. aureus Newman norfloxacin susceptibility profiles. Here, we observe that exposure to P. aeruginosa exoproducts leads to an increase in intracellular norfloxacin accumulation by S. aureus. We previously showed that P. aeruginosa supernatant dissipates the S. aureus membrane potential, and furthermore, depletion of the S. aureus proton motive force recapitulates the effect of the P. aeruginosa PA14 supernatant on shifting norfloxacin sensitivity profiles of biofilm-grown S. aureus Newman. From these results, we hypothesize that exposure to P. aeruginosa PA14 exoproducts leads to increased uptake of the drug and/or an impaired ability of S. aureus Newman to efflux norfloxacin. Surprisingly, the effect observed here of P. aeruginosa PA14 exoproducts on S. aureus Newman susceptibility to norfloxacin seemed to be specific to these strains and this antibiotic. Our results illustrate that microbially derived products can alter the ability of antimicrobial agents to kill bacterial biofilms. IMPORTANCE Pseudomonas aeruginosa and Staphylococcus aureus are frequently coisolated from multiple infection sites, including the lungs of individuals with cystic fibrosis (CF) and nonhealing diabetic foot ulcers. Coinfection with P. aeruginosa and S. aureus has been shown to produce worse outcomes compared to infection with either organism alone. Furthermore, the ability of these pathogens to form biofilms enables them to cause persistent infection and withstand antimicrobial therapy. In this study, we found that P. aeruginosa-secreted products dramatically increase the ability of the antibiotic norfloxacin to kill S. aureus biofilms. Understanding how interspecies interactions alter the antibiotic susceptibility of bacterial biofilms may inform treatment decisions and inspire the development of new therapeutic strategies.

scholarly journals Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nursenem Karaca ◽  
Görkem Şener ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antibacterial Effect ◽  
Synergistic Effects ◽  
Pharmaceutical Formulations ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
Antibacterial Effects ◽  
Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

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