scholarly journals Toksisitas Akut Ekstrak Metanol Mentimun (Cucumis Sativus L.) Terhadap Larva Artemia Salina Leach Metode Brine Shrimp Lethality Test (BSLT)

2019 ◽  
Vol 2 (2) ◽  
pp. 67
Author(s):  
Khairin Akbar Putra ◽  
Indah Purwaningsih ◽  
Kuswiyanto Kuswiyanto
Keyword(s):  
Acute Toxicity ◽  
Cucumis Sativus ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Early Screening ◽  
Microsoft Excel ◽  
Cucumis Sativus L ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

Abstract: Cucumber (Cucumis sativus L) is a plant that is widely consumed by the people. Beside due the delicious taste of it, cucumbers also contain some compounds that can be used as a drug, one of them is to prevent cancer. This plant contains Flavonoid, Saponin, and Tanin. These three compounds are the compounds which is in certain levels can be cytotoxic. Acute toxicity is an early screening test for a specifc cytotoxic potential crop for the development of anti-cancer drugs. The purpose of this research is to know the acute toxicity of cucumber (Cucumis sativus L) methanol extract to the Artemia salina Leach larvae by Brine Shrimp Lethality Test (BSLT) method. This research method was an experimental by using BSLT method. The test animal used was the Artemia salina Leach larvae. The method of extraction was maseration method. The results of the study can be seen from the percentage of larvae mortality for each concentration of extract, those are 0 μg / ml, 250 μg / ml, 500 μg / ml, 750 μg / ml, 1000 μg / ml are 0%, 48%, 62 %, 72%, 84% and 92%. Based on the result of probit analysis with microsoft Excel showed that LC50 value of cucumber methanol extract is 201,0165 μg /ml which means this extract have potency of acute toxicity because LC50 value <1000 μg / ml.Abstrak: Mentimun (Cucumis sativus L) adalah tanaman yang banyak dikonsumsi oleh masyarakat. Selain rasanya yang enak, mentimun juga memiliki kandungan senyawa yang dapat bermanfaat sebagai obat, salah satunya adalah untuk mencegah terjadinya kanker. Tanaman ini mengandung Flavonoid, Saponin, dan Tanin. Ketiga senyawa ini merupakan senyawa yang dengan kadar tertentu dapat bersifat sitotoksik. Toksisitas akut merupakan skrining awal untuk menguji suatu tanaman tertentu yang memiliki potensi sitotoksik untuk pengembangan obat anti kanker. Tujuan penelitian ini untuk mengetahui toksisitas akut dari ekstrak metanol mentimun (Cucumis sativus L) terhadap larva Artemia salina Leach dengan metode Brine Shrimp Lethality Test (BSLT). Metode penelitian ini berbentuk eksperimental dengan menggunakan metode BSLT. Hewan uji yang digunakan adalah larva Artemia salina Leach. Metode ekstraksi yang digunakan adalah metode maserasi. Hasil penelitian dapat dilihat dari persentase kematian larva untuk setiap konsentrasi ekstrak, yaitu 0 µg/ml, 250 µg/ml, 500 µg/ml, 750 µg/ml, 1000 µg/ml secara berturut-turut adalah 0%, 48%, 62%, 72%, 84% dan 92%. Berdasarkan hasil analisis probit dengan microsoft Excel menunjukkan nilai LC50 ekstrak metanol mentimun adalah 201, 0165 µg/ml yang berarti ekstrak ini memiliki potensi toksisitas akut karena nilai LC50 < 1000 µg/ml.

scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

scholarly journals UJI TOKSISITAS AKUT INFUSA DAUN SIRSAK (Annona muricata) PADA LARVA Artemia salina MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2020 ◽  
Vol 3 (1) ◽  
pp. 23
Author(s):  
Arinta Mayang ◽  
Bilal S A Santoso
Keyword(s):  
Acute Toxicity ◽  
Medicinal Plant ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Annona Muricata ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Chemical Contents

ABSTRACTAnnona muricata is a family of Annonaceae that has been known as a medicinal plant. Sirsakleaves contain alkaloids, tannins, and several other chemical contents including acetogenin.The aim of this study was to determine the acute toxicity of the infusion of Sirsak leaves onthe Artemia salina larvae by the Brine Shirmp Lethality Test (BSLT) method. This experimentalstudy used 5 treatment concentrations (10 mg/L, 50 mg/L, 100 mg/L, 500 mg/L, 1000mg/L) and 1 negative control. All treatments were repeated 3 times. The number of Artemiasalina larvae used for each concentration was 10 larvae. The number of dead larvae wascounted after 24 hours of treatment. Based on probit analysis, the LC50 value of Sirsak leafinfusion was 38,73 mg/L. The conclusion of this research is the infusion of Sirsak leaves istoxic.Keywords: Sirsak leaves, toxicity, BSLTABSTRAKSirsak (Annona muricata) adalah keluarga Annonaceae yang telah dikenal sebagai tanamanobat. Daun Sirsak mengandung alkaloid, tanin, dan beberapa kandungan kimia lainnya termasukacetogenin. Tujuan dari penelitian ini adalah untuk menentukan toksisitas akut dari infusdaun Sirsak pada larva Artemia salina dengan metode Brine Shirmp Lethality Test (BSLT).Studi eksperimental ini menggunakan 5 konsentrasi perlakuan (10 mg/L, 50 mg/L, 100 mg/L,500 mg/L, 1000 mg/L) dan 1 kontrol negatif. Semua perawatan diulang 3 kali. Jumlah larvaArtemia salina yang digunakan untuk setiap konsentrasi adalah 10 larva. Jumlah larva matidihitung setelah 24 jam perlakuan. Berdasarkan analisis probit, nilai LC50 infus daun Sirsakadalah 38,73 mg/L. Kesimpulan penelitian ini adalah infus daun Sirsak bersifat toksik.Kata kunci: daun sirsak, toksisitas, BSLT

scholarly journals ANALISIS FITOKIMIA DAN PENENTUAN NILAI LC50 EKSTRAK METANOL DAUN LIWAS

2014 ◽  
Vol 14 (2) ◽  
pp. 125
Author(s):  
Gerry Sumihe ◽  
Max R. J. Runtuwene ◽  
Johnly A. Rorong
Keyword(s):  
Secondary Metabolites ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

ABSTRAK Penentuan nilai LC50 dari ekstrak metanol daun liwas menggunakan metode Brine Shrimp Lethality Test (BSLT) terhadap hewan indikator udang Artemia salina menunjukkan nilai LC50 dari  A. salina bersifat sangat toksik.Nilai LC50 yang didapatkan sebesar 15,696 mg/L. Nilai LC50 ditentukan dengan menggunakan metode SPSS 20.0.Ekstrak metanol daun liwas mengandung kandungan senyawa metabolit sekunder seperti flavonoid, tanin dan saponin sebagai hasil uji fitokimia. Kata kunci: BSLT, Daun Liwas, Metabolit Sekunder,LC50.   PHYTOCHEMICAL ANALYSIS AND DETERMINING LC50 VALUE OF LIWAS LEAVE METHANOL EXTRACT ABSTRACT Determination of LC50 of methanol extract of leaves liwas using Brine Shrimp Lethality Test (BSLT) against animal indicator shrimp Artemia salina showed LC50 values of  A. salina is highly toxic. LC50 values were obtained at 15.696 mg / L. LC50 values determined using SPSS 20.0. Liwas leaf methanol extract contains compounds secondary metabolites such as flavonoids, tannins and saponins as phytochemical test results. Keywords: BSLT, Leaves Liwas, Secondary Metabolites, LC50.

UJI TOKSISITAS AKUT EKSTRAK ETANOL AKAR KB (Coptosapelta tomentosa Valeton ex K.Heyne) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2017 ◽  
Vol 2 (2) ◽  
pp. 161
Author(s):  
Risa Supriningrum ◽  
Sapri Sapri ◽  
Vici Ali Pranamala
Keyword(s):  
Acute Toxicity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

The roots of KB plants (Captosapelta tomentisa) is used empiricallyby  Dayak Kenyah community as a drug leukorrhea. Leukorrhea is one of the early symptoms of cervical cancer. Early methods to determine the LC50 value or toxicity of anticancer KB roots have not been reported. The aim of research is to determine the value of the toxicity of KB root. Research was conducted by an experimental study. Research stages include sample processing, extraction and acute toxicity test by Brine Shrimp Lethality Test (BSLT). BSLT testing to determine LC50 values with a concentration of 250 ppm, 300 ppm, 400 ppm, 450 ppm and a negative control. Analysis of the data used are probit analysis method. The results showed  the presence of flavonoids, alkaloids and saponins in of the ethanol extract of KB root . The test results obtained acute toxicity LC50 value is 299.226 mg / ml, so that the ethanol extract of roots KB potentially toxic to the larvae of Artemia salina Leach.

scholarly journals TOKSISITAS AKUT KOMBUCHA DAUN TIN (Ficus carica) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

2020 ◽  
Vol 3 (1) ◽  
pp. 11
Author(s):  
Uswatun Uswatun ◽  
Ernanin Dyah Wijayanti
Keyword(s):  
Secondary Metabolites ◽  
Acute Toxicity ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Ficus Carica ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

ABSTRACTFig leaves (Ficus carica) contain secondary metabolites such as flavonoids, tannins, alkaloids,saponins and triterpenoids, which potentially toxic in a certain amount. Fermentation of figleaves using kombucha produce healthy drink with various bioactivities. The aim of this researchwas to observe acute toxicity of fig leaves kombucha using Brine Shrimp LethalityTest (BSLT) method. Toxixity test againts Artemia salina Leach larvae using 7 variations infig leaves kombucha concentration from 100 ppm to 30000 ppm. The results showed that figleaves kombucha LC50 value of 139,99 ppm, so that fig leaves kombucha is potentially toxic.Key words: acute toxicity, BSLT, fermentation, fig leavesABSTRAKDaun tin (Ficus carica) mengandung metabolit sekunder antara lain flavonoid, tanin, alkaloid,saponin dan triterpenoid yang berpotensi toksik dalam jumlah tertentu. Fermentasi dauntin oleh kultur kombucha menghasilkan minuman kesehatan dengan berbagai bioaktivitas.Penelitian ini bertujuan untuk mengetahui toksisitas akut kombucha daun tin menggunakanmetode Brine Shrimp Lethality Test (BSLT). Pengujian toksisitas terhadap larva Artemia salinaLeach menggunakan 7 variasi konsentrasi kombucha daun tin antara 100 ppm sampai30000 ppm. Hasil penelitian menunjukkan nilai LC50 kombucha daun tin sebesar 139,99ppm, sehingga disimpulkan bahwa kombucha daun tin berpotensi toksik.Kata kunci: BSLT, daun tin, fermentasi, toksisitas akut

scholarly journals UJI TOKSISITAS AKUT MINUMAN PROBIOTIK SIRSAK GUNUNG (Annona montana Macf.) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

2020 ◽  
Vol 1 (5) ◽  
pp. 941-948
Author(s):  
Ambar Fidyasari ◽  
Sentot Joko Raharjo ◽  
Melani Setyowati
Keyword(s):  
Acute Toxicity ◽  
Traditional Medicine ◽  
Brine Shrimp ◽  
Experimental Methods ◽  
Test Method ◽  
Toxic Effects ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
A Value ◽  
Acute Toxic

Soursop fruit (Annona montana Macf.) is one of the plants can be used as as traditional medicine. This plant contains terpenoid and acetogenin which can cause toxicity. The fruit has a flavor that is tasteless so the innovation becomes probiotic drinks. This drink has been proven as an antioxidant, antibacterial, antihyperuricemia and antidiarrheal. The aim of this study was to know about acute toxicity of probiotic drink of soursop juice using brine shrimp lethality test method which will be indicated by LC50 value. This study used experimental methods conducted in the Laboratory of Farmakoknosi. There are several variations in concentration in this study, namely 10000 ppm, 20000 ppm, 30000 ppm, 40000 ppm, 50000 ppm, 60000 ppm, 70000 ppm, 80000 ppm and replication was done 3 times with total number of test animals used was 270. The results showed that probiotic drink of soursop juice can provide acute toxic effects on test animals with LC50 value of 29717,23 ppm. LC50 values ​​indicate that the mountain soursop probiotic drink is not potentially toxic because it has a value of >1000 ppm.

Toksisitas Akut Ektrak N-Heksan Dan Methanol Rimpang Jeringau Merah Terhadap Kematian Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test

2018 ◽  
Vol 1 (2) ◽  
pp. 125
Author(s):  
Edy Suwandi ◽  
Supriyanto Supriyanto
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Endemic Plant ◽  
Brine Shrimp Lethality ◽  
Purposive Sampling ◽  
Study Results ◽  
Sweet Flag

Abstract: Acorus sp (red sweet flag) is an endemic plant from West Borneo which contains chemical substances such as flavonoids that have bioactivity as antioxidants so it is recommended one to prevent cancer. The study aimed to determine toxicity of n-Hexan extract and methanol red sweet flag rhizomes against Artemia salina Leach with Brine Shrimp Lethality Test method. This study was a type of quasi experimental design, sampling technique obtained by using purposive sampling. According to the study results, it can be seen than methanol extract from red sweet flag rhizomes give impact on the mortality average of Artemia salina Leach : concentration 100 µg/ml (51%), 250 µg/ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) and 1000 µg/ml (99%). In addition, n-Hexan extract of red sweet flag rhizomes in 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) and 1000 µg/ml (96%). It is reported based on probit analysis that LC50 of n-Hexan extract from red sweet flag rhizomes was 128.2921 µg/ml meanwhile methanol extract was 242.7169 µg/ml µg/ ml, thus in conclusion n-Hexan and methanol extract of red sweet flag rhizomes potentially cytotoxic to Artemia salina Leach larvae.Abstrak: Jeringau merah (Acorus sp) merupakan satu jenis tanaman endemik Kalimantan Barat memiliki kandungan kimia diantaranya flavonoidyang mempunyai bioaktivitas sebagai antioksidan sehingga sangat baik untuk pencegahan kanker. Penelitian ini bertujuan mengetahui mengetahui dan menentukan toksisitas ekstrak n-Heksan dan Methanol Rimpang Jeringau MerahterhadapArtemia salinaLeachdengan metode Brine Shrimp Lethality Test. Desain penelitian ini adalah eksperimental semu, teknik pengambilan sampel dengan cara purposive sampling. Berdasarkan hasil penelitian diketahui ekstrak Methanol Rimpang Jeringau Merah rerata kematianArtemia salina Leach konsentrasi 100 µg/ml (51%), 250 µg/ ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) dan 1000 µg/ml (99%)pada ekstrak n-Heksan Rimpang Jeringau Merah konsentrasi 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) dan 1000 µg/ml (96%). Dari analisis probit diketahuinilai LC50 ekstrak n-Heksan Rimpang Jeringau Merah yaitu 128.2921 µg/ml dan ekstrak Methanol Rimpang Jeringau Merah yaitu 242.7169 µg/ml µg/ml, maka disimpulkan ekstrak n-Heksan dan Methanol Rimpang Jeringau Merahbersifat sitotoksik terhadap larva Artemia salina Leach.

scholarly journals Uji Toksisitas Ekstrak Batang Pinang Yaki (Areca vestiaria) pada Artemia salina Leach.

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Windy Astuti Tampungan
Keyword(s):  
Medicinal Plant ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Toxicity Level ◽  
Supernatant Solution

AbstrakEfek toksisitas pinang yaki (Areca vestaria) sebagai tumbuhan obat potensial perlu diuji untuk mengetahui ambang batas penggunaannya. Penelitian ini bertujuan untuk menguji tingkat toksisitas ekstrak batang pinang yaki terhadap larva Artemia salina Leach. dengan metode Brine Shrimp Lethality Test. Ekstrak diencerkan dengan konsentrasi 200, 400, 600 dan 800 ppm masing-masing untuk supernatan rendaman pertama (larutan A),supernatan rendaman ke dua (larutan B) dan supernatan rendaman ke tiga (larutan C) . Hasil penelitian menunjukkan bahwa ekstrak batang pinang yaki mempunyai efek toksisitas dengan nilai LC50 sebesar 398,28 ppm untuk larutan A, 390,84 ppm untuk larutan B dan 438,53 ppm untuk larutan C.Kata kunci: batang Areca vestaria,LC50, uji toksisitasAbstractToxicity effect of pinang yaki (Areca vestiaria) as potential medicinal plant should be evaluated to know its application threshold. This research aimed to evaluate the toxicity level of pinang yaki trunk extract on larve of Artemia salina Leach. using the method of Brine Shrimp Lethality Test. Extract was diluted to concentration of 200, 400, 600 and 800 ppm each for solution A (first soaking supernatant), solution B (second soaking supernatant)and solution C (third soaking supernatant). The result of this research showed that extract of pinang yaki trunk had a toxic characteristic, with LC50 value was 398,28, 390,84, and 438,53 ppm respectively for solution A, B and C.Keywords: LC50 , toxicity test, trunk of Areca vestaria

scholarly journals Synthesis and cytotoxicity assay using Brine Shrimp Lethality Test of Cinchonidine Isobutyrate Ester

2017 ◽  
Vol 19 (1) ◽  
pp. 29-35
Author(s):  
Mario Mario ◽  
Puspa Dewi Lotulung ◽  
Gian Primahana ◽  
Sylvia Rizky Prima ◽  
Muhammad Hanafi
Keyword(s):  
Fatty Acid ◽  
Brine Shrimp ◽  
Anticancer Agent ◽  
Isobutyric Acid ◽  
Artemia Salina ◽  
Cytotoxicity Assay ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Ft Ir ◽  
Pass Through

This research was aimed to synthesize cinchonidine isobutyrate ester and conduct a preliminary assay for anticancer agent using cytotoxicity assay to Artemia salina Leach larva, or also known as brine shrimp lethality test (BSLT). Cinchonidine, a compound that has quinoline rings and quiniclidine ring, is a quinine analogue and stereoisomer of cinchonine. Cinchonidine is predicted to have anticancer activity. Synthesized ester was aimed to gain higher lipophilicity. Higher lipophilicity makes it easier for the compund to pass through cell membrane. The esterification process used DMAP as a catalyst, DCC as an activator, and isobutyric acid as a carboxilyc acid. Isobutyric acid is a type of short chained fatty acid that usually acts as an anticancer prodrugs. The product is identified by ESI-MS, FT-IR, 1H-NMR, dan 13C-NMR. Ester cinchonidine isobutyrate is gelatinous and colourless with yield of 21,77%. BSLT result showed that cinchonidine isobutyrate ester had LC50 value of 75.16 ppm which was more toxic than cinchonidine that had LC50 value of 99.2 ppm. It was proved that higher lipophilicity could increase pharmacology activity

scholarly journals Uji Toksisitas Akut Ekstrak Spons Laut (Callyspongia aerizusa) terhadap Larva Artemia salina Leach dengan Metode Brine Shrimp Lethality Test

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Refrando M. Sondakh ◽  
Jimmy Posangi ◽  
Pemsi M. Wowor
Keyword(s):  
Acute Toxicity ◽  
Marine Sponge ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Control Group ◽  
Control Group Design ◽  
Brine Shrimp Lethality ◽  
Toxicity Potential ◽  
Sponge Extract

Abstract: Indonesia has a great potential of marine biological resources. One of the coral reef ecosystems is marine sponge Callyspongia aerizusa that contains compounds of steroid, alkaloid, flavonoid, and terpenoid. In order to develop those substances as components of drugs, it is necessary to perform initial screening of its acute toxicity potential. This study was aimed to obtain the acute toxicity potential of marine sponge extract using Brine Shrimp Lethality Test (BSLT). This was an experimental study using the posttest only control group design. Samples were 180 larvae of Artemia salina Leach divided into 6 groups of 10 larvae. Each group was tested with three replications. The marine sponge extract final concentrations in the media which contained larvae consecutively in groups 1, 2, 3, 4, 5, and 6 were 1000 μg/ml, 500 μg/ml, 250 μg/ml, 100 μg/ml, 50 μg/ml, and 0 μg/ml as the negative control. The observation was focused to larvae that died 24 hours after the administration of the extract. LC 50 value of the marine sponge extract was analyzed by using probit analysis with SPSS 16.0. The results indicated that the extract final concentrations that killed the larvae were 1000 μg/ml, 500 μg/ml, and 250 μg/ml. The result of probit analysis indicated that LC 50 value of marine sponge extract was 992.468 μg/mL. Conclusion: The marine sponge extract had acute toxicity potential against Artemia salina Leach larvae according to BSLT with a LC 50 value <1000 μg/Ml.Keywords: Acute toxicity test, BSLT, LC 50, Callyspongia aerizusa marine sponge Abstrak: Indonesia memiliki potensi sumber daya alam hayati laut yang besar. Salah satu ekosistem terumbu karang ialah spons laut Callyspongia sp. yang mengandung senyawa steroid, alkaloid, flavonoid, dan terpenoid. Diperlukan skrining awal mengenai potensi toksisitas akut untuk pengembangan bahan baku obat. Penelitian ini bertujuan untuk mengetahui potensi toksisitas akut ekstrak spons Callyspongia sp. menggunakan Brine Shrimp Lethality Test (BSLT). Jenis penelitian ialah eksperimental dengan posttest-only control group design. Hewan uji ialah 180 ekor larva Artemia salina Leach yang dibagi dalam 6 kelompok, masing-masing terdiri dari 10 ekor. Tiap kelompok dilakukan pengulangan percobaan 3 kali. Ekstrak spons laut diberikan dalam media yang berisi larva. Konsentrasi akhir ekstrak dalam media yang berisi larva berturut-turut dalam kelompok 1, 2, 3, 4, 5, dan 6 ialah 1000 μg/ml, 500 μg/ml, 250 μg/ml, 100 μg/ml, 50 μg/ml dan 0 μg/ml sebagai kontrol negatif. Pengamatan dilakukan terhadap larva yang mati 24 jam setelah pemberian bahan uji. LC 50 ekstrak spons laut ditentukan dengan analisis probit menggunakan SPSS 16.0 for windows. Hasil penelitian menunjukkan beban konsentrasi ekstrak dalam media dapat membunuh larva secara berturut-turut dengan konsentrasi 1000 μg/ml, 500 μg/ml, dan 250 μg/ml. Hasil analisis probit menunjukkan nilai LC 50 dari ekstrak spons laut ialah 992,468 μg/mL. Simpulan: Ekstrak spons laut menunjukkan adanya potensi toksisitas akut terhadap larva Artemia salina Leach menggunakan BSLT dengan nilai LC50 <1000 μg/mL.Kata kunci: toksisitas, BSLT, spons laut Callyspongia aerizusa

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