scholarly journals The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats� Induced with CCl4

Sains Medika ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 7
Author(s):  
Catur Retno Lestari ◽  
Titiek Sumarawati ◽  
Taufiqurrachman Nasihun
Keyword(s):  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Interleukin 1 ◽  
Hepatic Cell ◽  
Control Group ◽  
Toxic Substances ◽  
Metabolic System ◽  
Control Group Design ◽  
Propolis Extract ◽  
Male Wistar Rats

Introduction:Liver exposure to toxic substances will disrupt the metabolic system. Carbon tetrachloride (CCl4) is a hepatotoxic substance causing a liver damage. Continuous multi-stage countercurrent extraction(CMCE) propolis extract contains active balsamic substances, caffeic acid, phenethyl esters and flavonoids.Objective: to prove the effect of CMCE propolis extract on IL-1 levels and the hepatocyte histopathology findingsof male wistar rats�induced by CCl4.Methods:In the posttest only control group design, the total sample of 28 rats was divided into 4 groups: positive control group (K-0; standard feed); group P-1 (propolis3.6 mg/200 g); P-2 (propolis7.2mg/200gr and P-3 (propolis14.4mg/200g) were respectively given for 14 days,yet those on the 14thday were given CCl4. The IL-1 level was measured with the ELISA method, while the male wistar rats�hepatocyte histopathology findings was determined by the HE staining methods. IL-1 levelswere analyzed usingOne Way Anova test,followed by the Post Hoc LSD test. Hepatic cell histopathology was analyzed usingKruskal Wallis, followed by Mann-Whitney U.Results:Post Hoc LSD analysis shows that the levels of IL-1 in P-1 (983.54 �19.67), P-2 (841.58�19.47), and P-3 (755.50�31.63) are significantlylower thanthosein K-0 (1131.69�14.91) withp<0.05. Mann-Whitney U analysis shows that the hepatocyte histopathology in the P-1 group (2.55�0.39), P-2 (1.24�0.94) and P-3 (1.20�0.93) is significantlylower thanthatinK-0 (2.94�0.16) with p<0.05.Conclusion: The administration of CMCE propolis extract may reduce IL-1 levels and improve the male wistar rats�hepatic cell histopathologyinduced with carbon tetrachloride (CCl4).

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

2021 ◽  
Vol 6 (2) ◽  
pp. 159
Author(s):  
Nabilah Nabilah ◽  
Irham Taufiqurrahman ◽  
Nolista Indah Rasyid
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Gel Effect ◽  
Index Measurement ◽  
Male Wistar Rats ◽  
Collagen Density ◽  
Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

scholarly journals Effect of single clove garlic on clinical symptoms of photoaging in wistar rats exposed with UV-B

2019 ◽  
Vol 2 (2) ◽  
pp. 40-44
Author(s):  
Nyoman Pratiwi Hapsari Dewi ◽  
I Gusti Ayu Dewi Ratnayanti ◽  
I Gusti Kamasan Nyoman Arijana ◽  
Ni Made Linawati
Keyword(s):  
Clinical Symptom ◽  
Wistar Rats ◽  
Clinical Symptoms ◽  
Light Exposure ◽  
Premature Aging ◽  
The Other ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Photoaging is a premature aging that occurs on skin due to the ultraviolet light exposure that causes the emergence of clinical symptoms, one of which is wrinkle. One kind of material that can prevent photoaging is antioxidant. A single clove garlic has the highest antioxidant activity compared to the other materials. Aim: This research aimed at investigating the effectivity of single clove garlic in inhibiting the clinical symptom of photoaging. Method: This research was an experimental research, utilizing pre-post-test control group design. The used sample were 30 male wistar rats which were divided into 6 experimental groups. All groups were exposed to UV-B light with the amount of 840 mJ/cm2. Control group (P1) was only exposed to UV-B while the other groups were treated with placebo cream (P2), sunblock (P3), 5% garlic cream (P4), 10% garlic cream (P5) and 20% garlic cream (P6) respectively. The clinical symptom in the form of wrinkle was observed by using dermascope and the observations were categorized based on Glogau Scale. The statistical analysis utilized Wilcoxon and Kruskal Wallis test which was followed by Mann Whitney test. Result: The result of the research showed that there was significant difference on the apparent wrinkle on P1 group and P3, P5 and P6. Meanwhile the comparison between P1, P2 and P4 was not significant. Conclusion: The single clove garlic extract can prevent photoaging and has the similar protective effect for the skin as of sunblock.

scholarly journals ROLE OF COMBINATION CASEIN AND LACTOFERRIN BOVINE’S COLLOSTRUM AS A PULP CAPPING ON MACROPHAGE EXPRESSION IN MALE WISTAR RATS

2021 ◽  
Vol 8 (2) ◽  
pp. 156
Author(s):  
Endah Ariyati Eko Ningtyas ◽  
Oedijani Santoso ◽  
Udadi Sadhana ◽  
Siti Sunarintyas
Keyword(s):  
Therapeutic Effect ◽  
Wistar Rats ◽  
Therapeutic Potential ◽  
Healing Process ◽  
Bovine Colostrum ◽  
Control Group ◽  
Control Group Design ◽  
Reparative Dentin ◽  
Post Test ◽  
Male Wistar Rats

ABSTRACTBackground: Inflammatory and/or non-inflammatory processes play a role in stimulating pulp repair and the formation of hard tissue, namely reparative dentin. Macrophages play a role in the pathogenesis and chronic inflammatory disorders. The combination casein lactoferrin of bovine colostrum as an immunomodulator has therapeutic potential. This study aims to determine the therapeutic effect and duration of application of the combination of casein lactoferrin of bovine colostrum, on the expression of macrophages as pulp capping.Method: This study was a true experimental laboratories post test only control group design, consisting of three groups of 60 male wistar rats with 4 observation times, namely day to day 7, 14, 21 and 28 each of 5 mice. The maxillary 1st molars were prepared until the roof of the pulp was exposed. Three groups, namely the combination of casein and lactoferrin bovine colostrum (CKL) and calcium hydroxide (K1) and the untreated group (K0). Each group was filled with glassionomer as a permanent restoration. The tissue was made histological preparations with hematoxylin-eosin staining and the number of macrophages were counted, then analyzed by two way ANOVA and post hoc LSD tests.Result: The results showed that the therapeutic effect and duration of application of the combination of casein and lactoferrin bovine colostrum on the expression of macrophages as pulp cappingConclusion: The combination of casein and lactoferrin of bovine colostrum as capping material can increase the number of macrophages in the healing process of dental pulp.

scholarly journals PENGARUH PEMBERIAN SAGU DIBANDING NASI TERHADAP BERAT BADAN TIKUS WISTAR

e-CliniC ◽  
2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Eny A. Watumlawar ◽  
Sarah M. Warouw ◽  
Stefanus Gunawan
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Wilcoxon Test ◽  
Control Group ◽  
Increase Body Weight ◽  
Control Group Design ◽  
Tissue Mass ◽  
Group Design ◽  
Post Test ◽  
Male Wistar Rats

Abstract: Weight is one measure that gives description of the tissue mass including body fluids. Weight change is nfluenced by nutriment consumption. Sago contains high carbohydrates that can increase body weight. This study aimed to obtain the effect of sago compared to rice to body weight of wistar rats. This was a random laboratory experimental pre-post test with control group design. Subjects were male wistar rats, aged 5-6 months. The rats were divided in two groups: rice group as control and sago group. Sago was cooked in papeda form as much as 75 g of dried sago and 300 mL water. The rats were fed for 2 weeks. Data were analyzed by using the Wilcoxon test. The results showed that the sago group showed an increase of body weight significantly (p=0,001) meanwhile the rice group lose body weight significantly (p=0,001). Conclusion: Sago can increase body weight of wistar rats significantly.Keywords: body weight, sago, riceAbstrak: Berat badan merupakan salah satu ukuran yang memberikan gambaran massa jaringan termasuk cairan tubuh. Salah satu yang memengaruhi berat badan yaitu dengan mengonsumsi makanan bergizi. Sagu memiliki kandungan karbohidrat (pati) yang besar dan dapat meningkatkan berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sagu terhadap berat badan dibanding nasi pada tikus wistar. Desain penelitian ini ialah eksperimental laboratorium acak pre-post test with control group design. Subjek penelitian ialah tikus wistar jantan berusia 5-6 bulan dibagi atas 2 kelompok: kelompok nasi (kontrol) dan kelompok sagu. Sagu dimasak dalam bentuk papeda sebanyak 75 g sagu kering dan 300 mL air. Tikus diperlihara selama 2 minggu. Berat badan diukur dengan menggunakan timbangan. Data dianalisis dengan uji Wilcoxon. Hasil penelitian menunjukkan bahwa pemberian sagu sebanyak 2 cc setiap hari selama seminggu mampu meningkatkan berat badan secara bermakna (p = 0,001) sedangkan pemberian nasi menurunkan berat badan (p=0,001). Simpulan: Pemberian sagu dapat meningkatkan berat badan tikus wistar secara bermakna.Kata kunci: berat badan, sagu, nasi

scholarly journals THE EFFECT OF SEMBUNG LEAF EXTRACT (BLUMEA BALSAMIFERA) ON THE NUMBER AND DIAMETER OF RATS LEYDIG CELLS INDUCED BY HIGH-FAT DIET

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Gede Widhiantara ◽  
Anak Agung Ayu Putri Permatasari ◽  
Putu Angga Wiradana
Keyword(s):  
Treatment Group ◽  
Leydig Cells ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Control Group Design ◽  
Core Diameter ◽  
Post Test ◽  
Male Wistar Rats ◽  
The Mean

This study aims to determine the morphology of Leydig cells in hyperlipidemic wistar rats after having administered with sembung (Blumea balsamifera) extract orally. This study utilised a randomized post-test only control group design. The sample in this study were 16 adult male wistar rats (Rattus novergicus) aged 3-4 months with a body weight of 150-200 grams, which were equally and randomly divided into two groups, namely control group (hyperlipidaemia and sterile aquadest) and treatment group (hyperlipidaemia and sembung leaf extract). Hyperlipidaemia was induced with lard administration for 50 days. Data were analysed statistically using the Independent t-test. The results showed that the number of Leydig cells in the treatment group was higher than the control group with 68.13 ± 1.89 and 55.63 ± 1.92 cells respectively (P <0.05). In addition, the mean Leydig cell core diameter of the control group, 5.00 ± 0.34 µm, was smaller compared to the treatment group which was 5.80 ± 0.20 µm (P <0.05). It can be inferred that sembung leaf extract provides a protective effect against damage to Leydig cells due to hyperlipidaemia.

scholarly journals The effect of black cumin oil (Nigella sativa) on the reproduction of male Wistar rats

2021 ◽  
Vol 1 (2) ◽  
pp. 45-49
Author(s):  
Syahran Wael ◽  
Didik Wahyudi ◽  
Tayeb Wael ◽  
Zaid Mohamed Jaber
Keyword(s):  
Wistar Rats ◽  
Nigella Sativa ◽  
Cellular Damage ◽  
Control Group ◽  
Control Group Design ◽  
Black Cumin ◽  
Group Design ◽  
Nigella Sativa Oil ◽  
Post Test ◽  
Male Wistar Rats

Nigella sativa oil is antioxidant compound has the effect that serves to prevent cellular damage. The effect of Nigella sativa oil in the motility and total count of spermatozoa wistar rats. Experimental research with the design of post test only control group design. Wistar rats consists of 24 head and divided into 4 groups consist of control and treatment group. The control group was distilled. The first treatment was of Nigella sativa oil everydays as much with dose 150 mg/kgbw, 250 mg/kgbw, and 350 mg/kgbw, for 16 days, . Statistic test for motility and count of sperm wistar rats use the Kruskal-Wallis followed by Mann Whitney test. Avarage value of motility in the control (21.67±9.832) its lower than treatment. In dose 350 mg/kgbw its highest (52.33±13.292) compare in the treatment 250 mg/kgbw (40.67±17.512) and 150 mg/kgbw (30.67±8.165). avarage value of count sperm in the control (130.83±41.877) its lower than treatments. In dose 350 mg/kgbw its highest (199.67±23.480) compare in the treatment 250 mg/kgbw (187.50±74.538) and 150 mg/kgbw (140.83±32.568). Administration of Nigella sativa oil occur to enhancement motility and number of spermatozoa wistar rats.

scholarly journals Attenuation of Carbon Tetrachloride – Induced Hepatoxicity by Dacryodes edulis Seeds Ethanolic Extract in Male Wistar Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 9490-9500
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Corn Oil ◽  
Ethanolic Extract ◽  
Seed Extract ◽  
Control Group ◽  
Once Daily ◽  
Male Wistar Rats ◽  
Dacryodes Edulis

Dacryodes edulis (African pear) seed has a long history of use in ethnomedicine. However, there is a paucity of information on its ameliorative potential on hepatic damage. In the present study, the potentials of D. edulis ethanolic seed extract (DET) in attenuating carbon tetrachloride (CCl4)-induced hepatotoxicity in male Wistar rats was assessed. Ninety male Wistar rats were divided into 3 groups (n = 10) designated: control, CCl4, and DET, respectively. Animals in the control group were administered 2 mL/kg body weight corn oil, while those assigned to CCl4 and DET groups were administered i.p. 30% CCl4 in corn oil (1:1 v/v) at 2 mL/kg. Treatment was administered once every 24 h consecutively for 72 h. Thereafter, control and CCl4 groups were treated once daily with 1 mL distilled water by gavage, while DET rats were treated once daily with 1500 mg/kg DET by gavage. At every 7 days interval and for 28 consecutive days, animals were starved overnight, weighed, and six rats from each group were sacrificed under anesthesia to collect blood and liver samples. After 28 days, remarkable improvements in feed intake, body weight, serum levels of hepatic function indicators, and hepatic histology were observed in DET rats compared to CCl4 rats. These results suggest a possible ameliorative potential of D. edulis ethanolic seed extract against CCl4-induced hepatic injury.

scholarly journals Uji efektivitas ekstrak daun sendok (Plantago major L.) terhadap waktu perdarahan pada tikus Wistar jantan (Rattus norvegicus)

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Abedneju R. Kainde ◽  
Damajanty H.C. Pangemanan ◽  
Bernart S.P. Hutagalung
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Bleeding Time ◽  
Wistar Rat ◽  
Control Group ◽  
P Value ◽  
Plantago Major ◽  
Control Group Design ◽  
Group Design ◽  
Male Wistar Rats

Abstract: Herbal plant is often used to treat any kind of disease. There are many kinds of plants in Minahasa used as traditional medicine, inter alia daun sendok (Plantago major L.). Daun sendok is often used to treat bleeding. This study was aimed to obtain the effectiveness of daun sendok extract on bleeding time in male Wistar rats. This was a true experimental study with a posttest only group design. This study was conducted at the Laboratory of Pharmacology Faculty of Medicine University of Sam Ratulangi. Total samples were 14 rats divided into 2 groups: control group and treatment group. Wounds of 2 mm depth were perfomed on the rat tails of the treatment group followed by application of daun sendok extract. The results showed that the average bleeding time of the control group was 51.87 seconds meanwhile of the treatment group was 29.67 seconds. The unpaired t-test between the two groups showed a p value of 0.000. Conclusion: Daun sendok (Plantago major L.) extract could reduce the bleeding time in male Wistar rats. Keywords: bleeding time, daun sendok, Wistar rat Abstrak: Tanaman obat seringkali digunakan untuk mengobati berbagai macam jenis penyakit. Di wilayah Minahasa terdapat banyak jenis tanaman yang digunakan sebagai obat tradisional, salah satunya ialah daun sendok (Plantago major L.) yang seringkali digunakan untuk mengobati perdarahan. Penelitian ini berrtujuan untuk mengetahui efektivitas ekstrak daun sendok terhadap waktu perdarahan tikus Wistar jantan. Jenis penelitian ialah true experimental dengan post test only control group design yang dilakukan di Laboratorium Farmakologi Fakultas Kedokteran Unsrat dengan total sampel berjumlah 14 ekor tikus Wistar yang dibagi menjadi 2 kelompok yaitu: kelompok kontrol dan kelompok perlakuan menggunakan ekstrak daun sendok. Pada kelompok uji diaplikasikan ekstrak daun sendok setelah dilakukan perlukaan di bagian ekor tikus sedalam 2 mm. Hasil penelitian memperlihatkan rerata waktu perdarahan pada kelompok kontrol ialah 51,87 detik sedangkan pada kelompok perlakuan 29,67 detik. hasil uji t tidak berpasangan antara kedua kelompok mendapatkan nilai p=0,000. Simpulan: Ekstrak daun sendok (Plantago major L.) efektif dalam memperpendek waktu perdarahan pada tikus Wistar.Kata kunci: waktu perdarahan, daun sendok, tikus Wistar

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