scholarly journals CONTRIBUTIONS OF NURSES IN BASIC RESEARCH: DRESSING FIXATION MODEL FOR EXCISIONAL CUTANEOUS WOUNDS OF MICE

2021 ◽  
Author(s):  
Gilmara Lopes Amorim ◽  
Mariana Raquel Soares Guillen ◽  
Puebla Cassini Vieira ◽  
Eline Lima Borges
Keyword(s):  
Animal Model ◽  
Adverse Event ◽  
Basic Research ◽  
Preclinical Study ◽  
Body Structure ◽  
Dorsal Region ◽  
Cutaneous Wounds ◽  
Polyurethane Film ◽  
Adhesive Bandage ◽  
Made In

Objective: validate method of fixation of dressings on excisional cutaneous wounds of mice. Method: preclinical study. Sample made up of animals of the C57BL/6 strain, which had two excision wounds made in the dorsal region. Different methods and products, widely accepted in clinical practice, for fixing dressings in the animal model were evaluated. The evaluated outcomes were the length of stay of the dressing and the occurrence of adverse events. Results: crepe bandage, microporous tape and self adhesive bandage had a shorter residence time when compared to polyurethane film. This, in turn, varied the time when comparing different marks (E, F, G and H) and number of turns around the animal’s body. With 1 lap, the time varied from <24 to 36 hours. With 2 laps, the marks E and G remained 48 and 96 hours, respectively, and F and H time <24 hours. G-brand film, cut to size 3 cm x 15 cm, giving the mouse body 2 turns, kept the dressing for 96 hours. The skin remained intact, with no adverse event. Conclusion: a dressing fixation model for wounds in mice was created with a product available in Brazil and compatible with the animal’s body structure.

scholarly journals Application of Mass Spectrometry in Pancreatic Cancer Translational Research

2021 ◽  
Vol 11 ◽  
Author(s):  
Peng Ge ◽  
Yalan Luo ◽  
Haiyang Chen ◽  
Jiayue Liu ◽  
Haoya Guo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pancreatic Cancer ◽  
Malignant Tumors ◽  
Basic Research ◽  
Present Situation ◽  
Diagnosis And Treatment ◽  
Research Model ◽  
Comprehensive Diagnosis ◽  
Remarkable Progress ◽  
Made In

Pancreatic cancer (PC) is one of the most common malignant tumors in the digestive tract worldwide, with increased morbidity and mortality. In recent years, with the development of surgery, chemotherapy, radiotherapy, targeted therapy, and immunotherapy, and the change of the medical thinking model, remarkable progress has been made in researching comprehensive diagnosis and treatment of PC. However, the present situation of diagnostic and treatment of PC is still unsatisfactory. There is an urgent need for academia to fully integrate the basic research and clinical data from PC to form a research model conducive to clinical translation and promote the proper treatment of PC. This paper summarized the translation progress of mass spectrometry (MS) in the pathogenesis, diagnosis, prognosis, and PC treatment to promote the basic research results of PC into clinical diagnosis and treatment.

scholarly journals Clinical and Neurobiological Aspects of TAO Kinase Family in Neurodevelopmental Disorders

2021 ◽  
Vol 14 ◽  
Author(s):  
Chun Hu ◽  
Pan Feng ◽  
Qian Yang ◽  
Lin Xiao
Keyword(s):  
Neurodevelopmental Disorders ◽  
Basic Research ◽  
Genetic Data ◽  
Review Article ◽  
Biological Processes ◽  
Behavioral Impairment ◽  
Clinical Investigations ◽  
Substantial Progress ◽  
Biochemical Analyses ◽  
Made In

Despite the complexity of neurodevelopmental disorders (NDDs), from their genotype to phenotype, in the last few decades substantial progress has been made in understanding their pathophysiology. Recent accumulating evidence shows the relevance of genetic variants in thousand and one (TAO) kinases as major contributors to several NDDs. Although it is well-known that TAO kinases are a highly conserved family of STE20 kinase and play important roles in multiple biological processes, the emerging roles of TAO kinases in neurodevelopment and NDDs have yet to be intensively discussed. In this review article, we summarize the potential roles of the TAO kinases based on structural and biochemical analyses, present the genetic data from clinical investigations, and assess the mechanistic link between the mutations of TAO kinases, neuropathology, and behavioral impairment in NDDs. We then offer potential perspectives from basic research to clinical therapies, which may contribute to fully understanding how TAO kinases are involved in NDDs.

Multimodal optical imaging

2003 ◽  
Vol 285 (2) ◽  
pp. L269-L280 ◽  
Author(s):  
Cindy Lawler ◽  
William A. Suk ◽  
Bruce R. Pitt ◽  
Claudette M. St. Croix ◽  
Simon C. Watkins
Keyword(s):  
Optical Imaging ◽  
Imaging Techniques ◽  
Basic Research ◽  
Optical Methods ◽  
Resonance Imaging ◽  
X Ray ◽  
Positron Emission ◽  
Technological Advances ◽  
Visual Imaging ◽  
Made In

The recent resurgence of interest in the use of intravital microscopy in lung research is a manifestation of extraordinary progress in visual imaging and optical microscopy. This review evaluates the tools and instrumentation available for a number of imaging modalities, with particular attention to recent technological advances, and addresses recent progress in use of optical imaging techniques in basic pulmonary research. 1 Limitations of existing methods and anticipated future developments are also identified. Although there have also been major advances made in the use of magnetic resonance imaging, positron emission tomography, and X-ray and computed tomography to image intact lungs and while these technologies have been instrumental in advancing the diagnosis and treatment of patients, the purpose of this review is to outline developing optical methods that can be evaluated for use in basic research in pulmonary biology.

Lessons from Worm Dendritic Patterning

2019 ◽  
Vol 42 (1) ◽  
pp. 365-383 ◽  
Author(s):  
Sharon Inberg ◽  
Anna Meledin ◽  
Veronika Kravtsov ◽  
Yael Iosilevskii ◽  
Meital Oren-Suissa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Light And Electron Microscopy ◽  
Dendritic Tree ◽  
Model System ◽  
Model Organisms ◽  
Structural Units ◽  
The Past ◽  
Structural And Functional Properties ◽  
Made In

The structural and functional properties of neurons have intrigued scientists since the pioneering work of Santiago Ramón y Cajal. Since then, emerging cutting-edge technologies, including light and electron microscopy, electrophysiology, biochemistry, optogenetics, and molecular biology, have dramatically increased our understanding of dendritic properties. This advancement was also facilitated by the establishment of different animal model organisms, from flies to mammals. Here we describe the emerging model system of a Caenorhabditis elegans polymodal neuron named PVD, whose dendritic tree follows a stereotypical structure characterized by repeating candelabra-like structural units. In the past decade, progress has been made in understanding PVD's functions, morphogenesis, regeneration, and aging, yet many questions still remain.

Use of mesenchymal stem cells from amniotic fluid: preclinical study on animal model

2011 ◽  
Vol 40 (10) ◽  
pp. 1216
Author(s):  
S. Tetè ◽  
M. Tumedei ◽  
V. Zizzari ◽  
U. Di Tore ◽  
R. Grilli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Preclinical Study

Concurrent editing of automotive styling and structure with wireframe-pair

2016 ◽  
Vol 231 (6) ◽  
pp. 828-841 ◽  
Author(s):  
Baojun Li ◽  
Lei Yang ◽  
Hui Jiang ◽  
Weixue Sun ◽  
Ping Hu
Keyword(s):  
The Other ◽  
Free Form ◽  
Body Structure ◽  
Element Mesh ◽  
Mesh Model ◽  
Free Form Deformation ◽  
Joint Mapping ◽  
Deformation Transfer ◽  
Analysis Models ◽  
Made In

A constant demand in engineering design is to couple aesthetics and functionality of design. In this paper, a method is proposed to rapidly update the structural analysis models given the modifications made in the styling/packaging designs, forming a concurrent mechanism for developing the automotive styling and body structure. The seemingly disparate domains are represented by and thus coupled through a pair of wireframes. A joint mapping based on the hard points in packaging and a part mapping encoding local influences between wireframes are established. The deformation transfer from one of the wireframes to the other is solved as an optimization with constraints obtained from the two mappings. Finally, the finite element mesh model is adapted in accordance with the deformed structural wireframe, using a mesh morphing method based on the free-form deformation technique. Numerical results validate the proposed method and demonstrate its effectiveness.

scholarly journals Cytomorphological and Cytochemical Identification of Microglia

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Subhajit Das Sarma ◽  
Koushik Chatterjee ◽  
Himadri Dinda ◽  
Dhriti Chatterjee ◽  
Jayasri Das Sarma
Keyword(s):  
Central Nervous System ◽  
Animal Model ◽  
Immune Cells ◽  
Microglial Activation ◽  
Neurological Diseases ◽  
Neuronal Dysfunction ◽  
Ultrastructural Studies ◽  
The Central Nervous System ◽  
Made In

Microglia is one of the major resident immune cells in the central nervous system and is considered to be the key cellular mediator of neuroinflammatory processes. Identification of different Microglial states of activation by morphologic means has been one of the major challenges in the field of neurobiology of diseases. Therefore, microglial biology demands techniques to identify differing stages of microglia in different neuroanatomic locations as well as understanding the role of Microglia in different Neurological diseases. This present study is aimed towards summarizing the literature and for understanding the progress made in different Cytomorphological and Cytochemical techniques of identifying Microglia. This study also review recently used Immunohistochemistry techniques, along with Ultrastructural studies determining different morphological features of resting to activated phagocytic Microglia in a viral induced experimental animal model of neuroinflammation. Results revealed that chronic Microglial activation is considered to be an important component of neuronal dysfunction, injury, and loss (and hence to disease progression). Thus, Microglial research with special emphasis on identification of different activation states of Microglia has gradually become significant.

scholarly journals Protective Vaccination against Papillomavirus-Induced Skin Tumors under Immunocompetent and Immunosuppressive Conditions: A Preclinical Study Using a Natural Outbred Animal Model

2014 ◽  
Vol 10 (2) ◽  
pp. e1003924 ◽  
Author(s):  
Sabrina E. Vinzón ◽  
Ilona Braspenning-Wesch ◽  
Martin Müller ◽  
Edward K. Geissler ◽  
Ingo Nindl ◽  
...  
Keyword(s):  
Animal Model ◽  
Preclinical Study ◽  
Skin Tumors ◽  
Protective Vaccination

scholarly journals Animal Models of Uveal Melanoma: Methods, Applicability, and Limitations

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Marta M. Stei ◽  
Karin U. Loeffler ◽  
Frank G. Holz ◽  
Martina C. Herwig
Keyword(s):  
Animal Model ◽  
Animal Models ◽  
Uveal Melanoma ◽  
Tumor Biology ◽  
Scientific Progress ◽  
Basic Research ◽  
Research Progress ◽  
Transgenic Mouse Models ◽  
Tumor Patients ◽  
Xenograft Models

Animal models serve as powerful tools for investigating the pathobiology of cancer, identifying relevant pathways, and developing novel therapeutic agents. They have facilitated rapid scientific progress in many tumor entities. However, for establishing a powerful animal model of uveal melanoma fundamental challenges remain. To date, no animal model offers specific genetic attributes as well as histologic, immunologic, and metastatic features of uveal melanoma. Syngeneic models with intraocular injection of cutaneous melanoma cells may suit best for investigating immunologic/tumor biology aspects. However, differences between cutaneous and uveal melanoma regarding genetics and metastasis remain problematic. Human xenograft models are widely used for evaluating novel therapeutics but require immunosuppression to allow tumor growth. New approaches aim to establish transgenic mouse models of spontaneous uveal melanoma which recently provided preliminary promising results. Each model provides certain benefits and may render them suitable for answering a respective scientific question. However, all existing models also exhibit relevant limitations which may have led to delayed research progress. Despite refined therapeutic options for the primary ocular tumor, patients’ prognosis has not improved since the 1970s. Basic research needs to further focus on a refinement of a potent animal model which mimics uveal melanoma specific mechanisms of progression and metastasis. This review will summarise and interpret existing animal models of uveal melanoma including recent advances in the field.

Practical implementations for improving the throughput in a manual crystallization setup

2005 ◽  
Vol 38 (3) ◽  
pp. 568-570 ◽  
Author(s):  
Christian Biertümpfel ◽  
Jérôme Basquin ◽  
Dietrich Suck
Keyword(s):  
High Throughput ◽  
Structural Genomics ◽  
Basic Research ◽  
Financial Resources ◽  
The Past ◽  
Financial Investments ◽  
Simple Question ◽  
Speed Up ◽  
Reduction Of Costs ◽  
Made In

For the past few years, the number of structural genomics projects has been growing enormously worldwide. All these projects are supported by substantial financial resources and therefore are able to employ robotics for setting up high-throughput platforms. This paper addresses a simple question: how can basic research laboratories draw profit from the efforts and innovations that have been made to establish high-throughput facilities? To answer this question, the implementations that have been made in the authors' laboratory to improve manual crystallization setup with very limited financial investments are presented. In combination with 96-well microplates, an advanced protocol has been introduced and several simple devices have been designed to speed up different aspects of the manual crystallization setup, from storage of solutions to the setting of drops. These implementations lead to the reduction of costs in terms of time and money without any loss of quality. In addition, the crystallization throughput in the manual setup has been significantly increased.

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