scholarly journals Coconut water enhances immunity of cockerels before and after vaccination against Newcastle disease virus

2021 ◽  
Vol 6 (2) ◽  
pp. 58-63
Author(s):  
I. I. Bello ◽  
◽  
A. A. Abdullah ◽  
O. D. Adelakun ◽  
O. A. Agbato ◽  
...  
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Total Protein ◽  
Interleukin 2 ◽  
Immunoglobulin A ◽  
Serum Levels ◽  
Coconut Water ◽  
Ordinary Water ◽  
Before And After ◽  
Group B

Coconut water contains cytokinins and lauric acid which have potentials to enhance immune response. Poor immune response after vaccination is a common problem in poultry industry. Due to this challenge, this experimental study was carried out to evaluate the effects of coconut water on cell mediated and humoral immunity in cockerel chicks. Seventy five day-old cockerel chicks were randomly divided into three groups (A, B and C) and each group contained 25 birds. Group A (control) received ordinary water for 32 days while birds in group B received 100 ml of coconut water/1 L of water for 32 days. Group C received 100 mL of coconut water/1 L of water till day 19 of age and ordinary water from day 20 to day 32 of age. The birds were vaccinated against Newcastle disease (NDV) on day 20. Blood samples were collected on day 19 and 32 to evaluate the leukogram, antibody titer against NDV, total proteins and cytokines. Intestinal samples were also collected for immunoglobulin A analysis following the euthanasia of the birds. Means ± SD of the parameters were calculated and compared for significance differences using one-way ANOVA (p<0.05). A significantly lower total protein and globulin levels were recorded in groups B and C on day 19. Contrarily, there was an increase in total protein and globulin levels in groups B and C, and an increase in monocytes count in group B on day 32. The serum levels of gamma interferon, interleukin 2 and Newcastle disease antibody titre increased significantly in groups B and C on day 32. Immunoglobulin G and A concentrations were not significantly different across the three groups on day 32. These results showed that coconut water supplementation before and after vaccination improved immune response to Newcastle disease vaccine.

scholarly journals High serum and ascitic soluble interleukin-2 receptor alpha levels in advanced epithelial ovarian cancer [see comments]

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 424-429 ◽  
Author(s):  
DP Barton ◽  
DK Blanchard ◽  
B Michelini-Norris ◽  
SV Nicosia ◽  
D Cavanagh ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cancer Patients ◽  
Ascitic Fluid ◽  
Total Protein ◽  
Interleukin 2 ◽  
Serum Levels ◽  
Ca 125 ◽  
Soluble Interleukin 2 Receptor ◽  
Advanced Epithelial Ovarian Cancer

Abstract This study was undertaken to determine if advanced epithelial ovarian cancer was associated with increased serum and ascitic levels of soluble interleukin-2 receptor alpha (sIL-2R alpha). Serum and ascitic fluid samples from 23 ovarian cancer patients were analyzed for sIL-2R alpha using an enzyme-linked immunosorbent assay and compared with the serum and peritoneal levels in 18 normal females. The samples were analyzed for CA-125 levels using a radioimmunoassay and the total protein was also measured. Normal individuals had low serum levels of sIL-2R alpha (367.5 +/- 44.6 U/mL), with similar levels of sIL-2R alpha in the normal peritoneal fluid (438.6 +/- 48.8 U/mL). In contrast, the serum and ascitic fluid levels in ovarian cancer patients were significantly higher (746.7 +/- 82.9 U/mL, P = .0006; 2,656.7 +/- 373.7 U/mL, P = .00002, respectively). The results for sIL-2R alpha were also significant when the levels were expressed per milligram of total protein. More importantly, in almost every ovarian cancer patient the ascitic sIL-2R alpha level far exceeded the serum level, a pattern also observed for CA-125. There was no correlation between the serum and ascitic sIL-2R alpha levels, or between the serum and ascitic CA-125 levels. Although the serum levels of sIL-2R alpha and CA-125 were elevated in the same patient, overall there was no correlation between the serum sIL-2R alpha and serum CA-125 levels, either when the levels were expressed in absolute units or per milligram of total protein. Similarly, there was no correlation between sIL-2R alpha and CA-125 levels in individual ascitic samples. While CA-125 levels may reflect an independent index of tumor burden, these results suggest that selective accumulation of sIL-2R alpha in the ascites may be one of the factors associated with the known nonresponsiveness of the infiltrating lymphocytes against ovarian carcinoma cells.

INTRANASAL DELIVERY OF MANNAN-COATED LIPOSOMES ENHANCES ANTIBODY RESPONSES TO A NEWCASTLE DISEASE VIRUS VACCINE

2010 ◽  
Vol 22 (01) ◽  
pp. 1-7 ◽  
Author(s):  
Li-Ping Tseng ◽  
Chwei-Jang Chiou ◽  
Ming-Chung Deng ◽  
Yi-You Huang ◽  
Der-Zen Liu
Keyword(s):  
Immune Response ◽  
Survival Rate ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Immunoglobulin A ◽  
Adaptive Immune Response ◽  
Mucosal Vaccination ◽  
Adaptive Immune ◽  
Newcastle Disease Virus Vaccine

The polysaccharide, mannan, has been shown to be an effective immunoprotective agent and vaccine adjuvant in a variety of bacterial and viral diseases in different animal species. The objective of this study was to evaluate the immune response of chickens after being intranasal (i.n.) immunized using the Newcastle disease virus (NDV) encapsulated in liposomes and coated with mannan. Groups of chickens were vaccinated once intranasally with virus alone, then with uncoated liposome-encapsulated NDV (NC-lip), or mannan-coated liposome-encapsulated NDV (Man-lip). On day 14, velogenic viral challenge was carried out on vaccinated chickens. Chickens receiving virus alone had a 20% survival rate. In contrast, groups that received Man-lip had a higher survival rate of 70% (P < 0.01). The titers of mucosal secretary immunoglobulin A ( s-IgA ) was the highest in the group for the birds that received Man-lip as mucosal adjuvant (P < 0.01). These results suggest that liposomes coated with mannan may thus be used as a potential adjuvant for mucosal vaccination against NDV. This is the first study in which mannan has been demonstrated to enhance an adaptive immune response against ND virus infection in chickens.

scholarly journals Probiotics Stimulate Production of Natural Antibodies in Chickens

2006 ◽  
Vol 13 (9) ◽  
pp. 975-980 ◽  
Author(s):  
Hamid R. Haghighi ◽  
Jianhua Gong ◽  
Carlton L. Gyles ◽  
M. Anthony Hayes ◽  
Huaijun Zhou ◽  
...  
Keyword(s):  
Immune Response ◽  
Immunoglobulin A ◽  
Serum Levels ◽  
Natural Antibodies ◽  
Commensal Bacteria ◽  
Lymphoid Tissues ◽  
Alpha Toxin ◽  
Igg Antibodies ◽  
Significant Difference

ABSTRACT Commensal bacteria in the intestine play an important role in the development of immune response. These bacteria interact with cells of the gut-associated lymphoid tissues (GALT). Among cells of the GALT, B-1 cells are of note. These cells are involved in the production of natural antibodies. In the present study, we determined whether manipulation of the intestinal microbiota by administration of probiotics, which we had previously shown to enhance specific systemic antibody response, could affect the development of natural antibodies in the intestines and sera of chickens. Our findings demonstrate that when 1-day-old chicks were treated with probiotics, serum and intestinal antibodies reactive to tetanus toxoid (TT) and Clostridium perfringens alpha-toxin in addition to intestinal immunoglobulin A (IgA) reactive to bovine serum albumin (BSA) were increased in unimmunized chickens. Moreover, IgG antibodies reactive to TT were increased in the intestines of probiotic-treated chickens compared to those of untreated controls. In serum, IgG and IgM reactive to TT and alpha-toxin were increased in probiotic-treated, unimmunized chickens compared to levels in untreated controls. However, no significant difference in serum levels of IgM or IgG response to BSA was observed. These results are suggestive of the induction of natural antibodies in probiotic-treated, unimmunized chickens. Elucidating the role of these antibodies in maintenance of the chicken immune system homeostasis and immune response to pathogens requires further investigation.

scholarly journals EVALUATION OF MATERNALLY DERIVED ANTIBODIES AGAINST NEWCASTLE DISEASE VIRUS AND ITS EFFECT ON VACCINATION IN BROILER CHICKS

2010 ◽  
Vol 7 (2) ◽  
pp. 296-302 ◽  
Author(s):  
MA Jalil ◽  
MA Samad ◽  
MT Islam
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Humoral Immune Response ◽  
Broiler Chickens ◽  
Primary Vaccination ◽  
Challenge Infection ◽  
Broiler Chicks ◽  
Humoral Immune ◽  
Different Ages ◽  
Group B

The study was conducted to determine the persistence of maternally derived antibody (MDA) and its effects on protection against NDV in broiler chickens and to investigate the status of humoral immune response following vaccination with BCRDV® (F-strain, lentogenic) at different ages of broiler chickens during the period from August to October,  2008. A total of 90 day-old broiler chicks of Cobb 500 strain with the history of vaccination of parent stock against Newcastle disease (ND) was divided into three groups (A, B and C). Birds of group A (n = 35) were used for the study of protection ability of MDA against NDV, the birds of group B (n = 45) were used for the measurement of humoral immune response in chickens following vaccination at different ages and birds of group C (n = 10) were used for the determination of persistence of maternally derived antibody. The level of antibody titre against NDV was determined by HI test. The protective potentiality of MDA and vaccine was determined by the rate of survivability of the chickens following challenge infection. It was observed that the MDA titre in day-old chicks was higher and gradually declined at minimal level at day 28. The MDA titre of 128 or above protected the birds following challenge infection with virulent NDV. There were significant decrease in HI titres of chickens which were vaccinated once at day 1 and day 7, and could not withstand challenge infection with virulent NDV. Single vaccination with BCRDV® at day 14 triggered the production of antibody but could not provide complete protection to the birds. The birds which were boosted with the same vaccine 7 days and 21 days after primary vaccination produced better immune response. However, the birds which were vaccinated primarily at day 1 and boosted at day 7 could not withstand the challenge completely. Of the other regimens of twice vaccination, primary vaccination at day 7 and booster dosing at day 28 was found to be the best in terms of immune response and protection potentiality. Therefore, it may be concluded that (a) The MDA titre level of 128 or above is sufficient to protect broilers against challenge with virulent NDV,( b) Primary vaccination at day 7 followed by a booster dosing at day 28 may be followed for better immune response and protection against ND in broilers.DOI: 10.3329/bjvm.v7i2.5995Bangl. J. Vet. Med. (2009). 7(2) : 296 – 302

PSX-B-24 Influence of the immunomodulatory drug Azoxivet on the post-vaccination immune response in laying hens

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 269-269
Author(s):  
Valentina Sabrekova ◽  
Maxim Korenyuga ◽  
Elena Konovalova ◽  
Natalia Rodionova
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Laying Hens ◽  
Positive Influence ◽  
The Body ◽  
Infectious Bursal Disease ◽  
Post Vaccination ◽  
Before And After ◽  
Bursal Disease ◽  
Immunomodulatory Drug

Abstract Vaccination is a primary way to prevent infectious disease in poultry. The quality of immune response depends on the immune status of the body which, in turn, depends on many endogenous and exogenous factors. This study analyzed the effect of the immunomodulatory drug Azoxivet on the immune response in laying hens after vaccination against Newcastle Disease and Infectious Bursal Disease. There were 4 groups of Loman White cross laying hens (n=20). At age 120 days, all hens habitated in individual cages with an area of 0,15 m2. The conditions of keeping and feeding matched breed requirements. All groups were vaccinated against Newcastle Disease (NDV) (LaSota strain) and Infectious Bursal Disease (IBD) (Winterfield 2512 strain). All groups received Azoxivet (Az) at a dose of 0,3 mg/kg in water three times daily. Blood sera were collected weekly for serological studies using BioChek IBD and NDV test systems. The antibody level of all hens before and after vaccination: 1378 (IBD+Az) vs. 1674,93 (IBD) (P &lt; 0,05), 5194,75 (NDV+Az) vs. 5612,87 (NDV) (P &lt; 0,05). After one week of vaccination: 5931,25 (IBD+Az) vs. 5006,14 (IBD) (P &lt; 0,05), 6207,58 (NDV+Az) vs. 5765,21 (NDV) (P &lt; 0,05). Two weeks: 11086,00 (IBD+Az) vs. 10485,83 (IBD) (P &lt; 0,05), 11255,25 (NDV+Az) vs. 8478,75 (NDV) (P &lt; 0,05). Three weeks: 11478 (IBD+Az) vs. 8286 (IBD) (P &lt; 0,05), 14725 (NDV+Az) vs. 12677 (NDV) (P &lt; 0,05). Four weeks: 12999 (IBD+Az) vs. 1009,67 (IBD) (P &lt; 0,05), 17399 (NDV+Az) vs. 16373,17 (NDV) (P &lt; 0,05). Five weeks: 13601,15 (IBD+Az) vs. 9021,30 (IBD) (P &lt; 0,05), 19671 (NDV+Az) vs. 16309 (NDV) (P &lt; 0,05). Thus, Azaxul had a positive influence on the post-vaccination immune response in laying hens when used after Newcastle Disease and Infectious Bursal Disease vaccines. The results of the study can be used to improve disease prevention in poultry farming.

scholarly journals Local and Systemic Up-regulation of TNFα, IL-1β and IL-6 in Mice Intratracheally Inoculated with Porphyromonas gingivalis

2008 ◽  
Vol 77 (3) ◽  
pp. 377-385 ◽  
Author(s):  
Z. Pavlica ◽  
A. Nemec ◽  
A. Nemec-Svete ◽  
D. Eržen ◽  
D. A. Crossley ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Porphyromonas Gingivalis ◽  
Total Protein ◽  
Protein Concentration ◽  
Serum Levels ◽  
Treated Group ◽  
Control Group ◽  
Total Protein Concentration ◽  
Sterile Phosphate Buffer

The objective of the study was to find whether a single intratracheal inoculation with live Porphyromonas gingivalis ATCC 33277 influences local and systemic inflammatory and immune responses in mice.Twelve-week-old BALB/c mice were intratracheally inoculated with 2.9 × 109 CFU P. gingivalis ATCC 33277 diluted in 40 μl sterile phosphate buffer (treated group) or with sterile PBS (control group). The animals were sacrificed 2, 6, 24, 72 and 168 h after inoculation. TNFα, IL-1β, IL-6 and total protein concentrations were measured in the serum, lungs and kidneys. Six hours after P. gingivalis inoculation, TNFα concentration was significantly increased in serum (p = 0.02) and kidneys (p = 0.04), but in the lungs TNFα production was enhanced already 2 h (p < 0.0001) after inoculation, reaching the peak after 6 h (p < 0.0001). The IL-1β concentration was also significantly increased in serum after 2 h (p = 0.006), remaining significantly elevated up to 3 days (p ≤ 0.0001) after inoculation. In lungs IL-1β levels were significantly increased 6 and 24 h (p < 0.0001) and in kidneys 24 h (p < 0.0001) and 168 h (p = 0.01) after inoculation. The IL-6 concentration was significantly increased in serum after 72 and 168 h (p < 0.0001). However, IL-6 was significantly increased in lungs after 6 h (p < 0.0001), remaining elevated until 72 h and in kidneys 2 and 6 h (p < 0.0001) after inoculation. Significantly increased total protein concentration was detected in kidneys 6 and 24 h (p < 0.0001) after inoculation. These results suggest that a single intratracheal inoculation with P. gingivalis stimulates the local and systemic inflammatory and immune response, as shown by increased tissue and serum levels of proinflammatory cytokines.

scholarly journals Comparison of Immunologic Assays for Detecting Immune Responses in HIV Immunotherapeutic Studies: AIDS Clinical Trials Group Trial A5181

2010 ◽  
Vol 17 (9) ◽  
pp. 1452-1459 ◽  
Author(s):  
Bernard J. C. Macatangay ◽  
Lu Zheng ◽  
Charles R. Rinaldo ◽  
Alan L. Landay ◽  
Richard B. Pollard ◽  
...  
Keyword(s):  
Immune Response ◽  
Clinical Trials ◽  
T Cell ◽  
Lymphocyte Proliferation ◽  
Elispot Assay ◽  
Cytokine Production ◽  
Interleukin 2 ◽  
Ifn Γ ◽  
Group B ◽  
Hiv 1

ABSTRACT This study was designed to evaluate which of several T-cell-specific, immune response assays are the most relevant in measuring the key characteristics of an effective immune response to HIV-1. Using 5 HIV-1 antigens as stimulants, we assessed lymphocyte proliferation, supernatant gamma interferon (IFN-γ) cytokine production (CP), single-cell IFN-γ production by enzyme-linked immunospot (ELISPOT) assay, with and without Epstein-Barr virus-transformed B-lymphoblastoid cell lines (B-LCLs), and intracellular cytokine production (ICC) for IFN-γ and interleukin 2 (IL-2) by flow cytometry. We used these to compare specimens from HIV-1-infected subjects who were virally suppressed with a stable antiretroviral therapy (ART) regimen (group A) with specimens from subjects not on ART but with HIV-1 viremia of <3,000 copies/ml (group B). The lymphocyte proliferation assay (LPA) did not significantly differentiate between the two groups. Using fresh peripheral blood mononuclear cells (PBMCs), the CP and ELISPOT assays for IFN-γ detected the greatest differences between the two groups, specific for three of the five HIV-1 antigens, whereas significant differences were seen only in response to one antigen when cryopreserved cells were used. The strongest correlations were seen between the CP and ELISPOT assays. The ELISPOT B-LCL assay showed a cell concentration-dependent increase in IFN-γ production compared to that shown by the standard ELISPOT assay but did not differentiate between the groups. In the ICC assay, greater numbers of IFN-γ-producing T cells were seen in group B, and little or no detectable IL-2 production was seen in both groups. These studies highlight complexities of immunologic monitoring of T-cell responses in multisite clinical trials in HIV infection and outline considerations for optimizing these efforts.

scholarly journals Bone Demineralization in Postmenopausal Women: Role of Anamnestic Risk Factors

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Sandro La Vignera ◽  
Rosita A. Condorelli ◽  
Enzo Vicari ◽  
Chiara Nicoletti ◽  
Aldo E. Calogero
Keyword(s):  
Risk Factors ◽  
Postmenopausal Women ◽  
Nodular Goiter ◽  
Serum Levels ◽  
Fixed Dose ◽  
Mineral Density ◽  
Before And After ◽  
Group A ◽  
One Year ◽  
Group B

This study evaluated the effects of LT4 administration on the bone mineral density (BMD) in physiological postmenopausal women after two years of continuative treatment. 110 postmenopausal women with nodular goiter aged between 50 and 55 years were examined before and after 2 years of therapy with a fixed dose of LT4 (1.6 mcg/kg/die) for the treatment of nodular thyroid disease. The results showed that the patients on treatment with LT4 have a slight, but significant reduction of the BMD after 2 years of treatment, associated with increased serum levels of alkaline phosphatase and urinary excretion of hydroxyproline, confirming our data conducted on the same group after one year of therapy. Comparison between patients receiving LT4 (group A) or not (group B) showed that group A patients had significantly lower BMD. We demonstrated the statistically significant influence of the following risk factors on BMD: (1) body mass index<19 kg/m2; (2) the onset of menarche after the age of 15 years; (3) positive history for period of amenorrhoea; (4) nulliparity.

scholarly journals Immune response dynamics and Lutzomyia longipalpis exposure characterize a biosignature of visceral leishmaniasis susceptibility in a canine cohort

2021 ◽  
Vol 15 (2) ◽  
pp. e0009137
Author(s):  
Manuela da Silva Solcà ◽  
Maiara Reis Arruda ◽  
Bruna Martins Macedo Leite ◽  
Tiago Feitosa Mota ◽  
Miriam Flores Rebouças ◽  
...  
Keyword(s):  
Immune Response ◽  
Visceral Leishmaniasis ◽  
Clinical Manifestations ◽  
Parasite Load ◽  
Serum Levels ◽  
Lutzomyia Longipalpis ◽  
Response Dynamics ◽  
Before And After ◽  
One Year

Background Reports have shown correlations between the immune response to vector saliva and Leishmaniasis outcome. We followed dogs in an endemic area for two years characterizing resistance or susceptibility to canine visceral leishmaniasis (CVL) according to Leishmania infantum diagnosis and clinical development criteria. Then, we aimed to identify a biosignature based on parasite load, serum biological mediators’ interactions, and vector exposure intensity associated with CVL resistance and susceptibility. Methodology/Principal findings A prospective two-year study was conducted in an area endemic for CVL. Dogs were evaluated at 6-month intervals to determine infection, clinical manifestations, immune profile, and sandfly exposure. CVL resistance or susceptibility was determined upon the conclusion of the study. After two years, 78% of the dogs were infected with L. infantum (53% susceptible and 47% resistant to CVL). Susceptible dogs presented higher splenic parasite load as well as persistence of the parasite during the follow-up, compared to resistant ones. Susceptible dogs also displayed a higher number of correlations among the investigated biological mediators, before and after infection diagnosis. At baseline, anti-saliva antibodies, indicative of exposure to the vector, were detected in 62% of the dogs, reaching 100% in one year. Higher sandfly exposure increased the risk of susceptibility to CVL by 1.6 times (CI: 1.11–2.41). We identified a discriminatory biosignature between the resistant and susceptible dogs assessing splenic parasite load, interaction of biological mediators, PGE2 serum levels and intensity of exposure to sandfly. All these parameters were elevated in susceptible dogs compared to resistant animals. Conclusions/Significance The biosignature identified in our study reinforces the idea that CVL is a complex multifactorial disease that is affected by a set of factors which are correlated and, for a better understanding of CVL, should not be evaluated in an isolated way.

scholarly journals High serum and ascitic soluble interleukin-2 receptor alpha levels in advanced epithelial ovarian cancer [see comments]

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 424-429 ◽  
Author(s):  
DP Barton ◽  
DK Blanchard ◽  
B Michelini-Norris ◽  
SV Nicosia ◽  
D Cavanagh ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cancer Patients ◽  
Ascitic Fluid ◽  
Total Protein ◽  
Interleukin 2 ◽  
Serum Levels ◽  
Ca 125 ◽  
Soluble Interleukin 2 Receptor ◽  
Advanced Epithelial Ovarian Cancer

This study was undertaken to determine if advanced epithelial ovarian cancer was associated with increased serum and ascitic levels of soluble interleukin-2 receptor alpha (sIL-2R alpha). Serum and ascitic fluid samples from 23 ovarian cancer patients were analyzed for sIL-2R alpha using an enzyme-linked immunosorbent assay and compared with the serum and peritoneal levels in 18 normal females. The samples were analyzed for CA-125 levels using a radioimmunoassay and the total protein was also measured. Normal individuals had low serum levels of sIL-2R alpha (367.5 +/- 44.6 U/mL), with similar levels of sIL-2R alpha in the normal peritoneal fluid (438.6 +/- 48.8 U/mL). In contrast, the serum and ascitic fluid levels in ovarian cancer patients were significantly higher (746.7 +/- 82.9 U/mL, P = .0006; 2,656.7 +/- 373.7 U/mL, P = .00002, respectively). The results for sIL-2R alpha were also significant when the levels were expressed per milligram of total protein. More importantly, in almost every ovarian cancer patient the ascitic sIL-2R alpha level far exceeded the serum level, a pattern also observed for CA-125. There was no correlation between the serum and ascitic sIL-2R alpha levels, or between the serum and ascitic CA-125 levels. Although the serum levels of sIL-2R alpha and CA-125 were elevated in the same patient, overall there was no correlation between the serum sIL-2R alpha and serum CA-125 levels, either when the levels were expressed in absolute units or per milligram of total protein. Similarly, there was no correlation between sIL-2R alpha and CA-125 levels in individual ascitic samples. While CA-125 levels may reflect an independent index of tumor burden, these results suggest that selective accumulation of sIL-2R alpha in the ascites may be one of the factors associated with the known nonresponsiveness of the infiltrating lymphocytes against ovarian carcinoma cells.

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