scholarly journals Uji Bioaktivitas Antibakteri Senyawa murni dari Jamur Endofit Sporothrix sp Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

2019 ◽  
Vol 6 (1) ◽  
pp. 37
Author(s):  
Dewi Yudiana Shinta ◽  
Yusmarini Yusmarini ◽  
Herix Sonata MS ◽  
Hilwan Yuda Teruna ◽  
Saryono Saryono
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Positive Control ◽  
Significant Inhibition ◽  
New Drugs ◽  
Diffusion Method ◽  
Significant Difference ◽  
Pure Compounds ◽  
Escherichia Coli Bacteria

Modern medicines that are developing now come from active ingredients isolated from plants that require large amounts of plants. The development of new drugs from endophytic fungi found obstacles in the amount of pure compounds produced. Therefore further research is needed by using endophytic fungi as a new antimicrobial producer. This study aims to see the ability or activity of pure compounds produced by Sporothrix sp endophytic fungi from Dahlia tuber (Dahlia variabilis). Test the activity of pure compounds produced by Sporothrix sp. Endophytic fungi on E. coli and Staphylococcus aureus determined by disc diffusion method. With doses of 10, 30 and 50μg/disk. In Escherichia coli bacteria doses 10 and 50μg/disk gave significant inhibition of pure compounds from isolation compared to the positive control of ciprofloxacin, which was marked by a statistically significant test result (p <0.05). In contrast to Staphylococcus aureus there was no significant difference in doses of both doses of 10.30 and 50μg/disk. Determination of pure compounds was carried out by HPLC and Infra Red Spectrophotometry.

scholarly journals PREPARATION OF COLD CREAM AGAINST CLINICAL PATHOGEN USING CARALLUMA ADSCENDENS VAR. ATTENUATA

2020 ◽  
pp. 120-123
Author(s):  
SUNDAR MADASAMY ◽  
SURESH SUNDAN ◽  
LINGAKUMAR KRISHNASAMY
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Positive Control ◽  
Diffusion Method ◽  
Methicillin Resistant ◽  
Cold Cream ◽  
Significant Difference ◽  
Vancomycin Resistant

Objective: A simple formulation of cold cream from methanolic extract Caralluma adscendens var. attenuata (MECA) and their antimicrobial activity was tested against various clinical pathogens, namely, Escherichia coli, methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium, and Candida albicans. Methods: Methanol extract of these plant extract was prepared by the Soxhlet method. We analyzed phytochemical nature of theses plant, and subsequently, a cream was formulated cold-cream C. adscendens var. attenuata (FCA) different concentration such as FCA 50 mg, FCA 100 mg, and FCA 200 mg. In the present study, aimed to the antimicrobial activity of cold cream was measured by agar well diffusion method, and standard antibiotic Neosporin (market available) cream was used as positive control and dummy cold cream (without-MECA) were used as the negative control. Results: Phytochemical screening showed that the plant extracts were found a rich source of secondary metabolites. For more, the efficacy of cold cream from MECA extracts to against the clinical pathogen. Positive control Neosporin and 200 mg FCA cream was a highly significant difference in the zone of inhibition when compared to dummy cream. The 200 mg FCA was activity against Escherichia coli, methicillin-resistant Staphylococcus aureus, vancomycin-resistant E. faecium, and C. albicans highly significantly difference (p<0.05) compared FCA 50 mg and FAC 100 mg creams. Conclusion: The results from this study suggested that the cold cream form base of MECA crude had antimicrobial activity in the different clinical pathogen. They could be used as an alternative source to conventional antimicrobial agents for the treatment of pathological infection.

scholarly journals Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 201 ◽  
Author(s):  
Suryadi Budi Utomo ◽  
Mita Fujiyanti ◽  
Warih Puji Lestari ◽  
Sri Mulyani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Hexadecyltrimethylammonium Bromide ◽  
E Coli ◽  
Escherichia Coli Bacteria

<p>This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the <em>S.</em><em> </em><em>aureus</em> bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against <em>E. coli</em>.</p>

scholarly journals UJI EFEKTIVITAS ASAP CAIR CANGKANG BUAH Hevea braziliensis TERHADAP AKTIVITAS BAKTERI Escherichia coli

Alotrop ◽  
2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Dinda Oktarina ◽  
Sumpono Sumpono ◽  
Rina Elvia
Keyword(s):  
Escherichia Coli ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Clear Zone ◽  
Total Acid ◽  
Liquid Smoke ◽  
Significant Difference ◽  
Escherichia Coli Bacteria ◽  
Stage 1

This research purpose to determine the effectiveness of the liquid smoke of the Hevea braziliensis  fruit shell  against the activity of Escherichia coli bacteria. The preparation and purification of liquid smoke is carried out by 4 stages: pyrolysis , sedimentation, stage 1 and stage  2  distillation. This research was started with total acidity test, phenol level, pH and density with result of measurement 4,725% total acid content, 0,84% phenol content, 2,548 pH and 1,004 in density . An antibacterial test  using disc diffusion method by looking at the diameter of the clear zone. There are six concentrations of liquid smoke of  H.braziliensis  rubber shell used, negative control using aquades and positive control using ciprofloxacin . An  effective concentration was found in inhibiting E.coli is 100% concentration with a clear zone diameter of 11.3 mm. The result of analysis using One Way ANOVA showed F count (4.42)> F table (3,23), then H0 rejected, meaning there is a significant difference of influence of concentration to the diameter of clear zone produced.

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

scholarly journals ANTIBAKTERI SENYAWA FENOLIK DARI ALANG -ALANG (IMPERATA CYLINDRICA)

2021 ◽  
Vol 3 (2) ◽  
pp. 124-126
Author(s):  
Jhon Patar Sinurat ◽  
Suci Wulandari ◽  
Rinaldo Berutu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Phenolic Compounds ◽  
Screening Test ◽  
Inhibition Zone ◽  
Imperata Cylindrica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Agar Diffusion Method ◽  
Escherichia Coli Bacteria

Phytochemical screening test proved that the extract of Reeds (Imperata cylindrica) contained phenolic compounds tested using 5% FeCl3 reagent. Antibacterial test using agar diffusion method against Reeds extract in DMSO solvent. The phenolic compounds obtained from the saputangan leaves Reeds extract were 36.96 grams after undergoing maceration. Reeds extract of phenolic compounds with concentrations (200; 100; 50 and 25 ppm) had strength antibacterial where the average inhibition zone of Staphylococcus aureus bacteria was 10.0 mm and the average inhibition zone was at Escherichia coli bacteria measuring 10.3 mm.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADO

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 114
Author(s):  
Lyone Katiandagho ◽  
Defny S Wewengkang ◽  
Sri Sudewi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Soft Coral ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Water Fraction

ABSTRACT Soft Coral Sinularia sp.has been shown to have antibacterial activity. This research aims to determine the antibacterial activity of marine  organisms obtained from Manado bay. The method in this study is the diffusion method with positive control chloramphenicol and negative control of methanol in Escerichia coli and Sthaphylococcus aureus. The result showed that extracts, chloroform fraction and methanol-water fraction were obtained inhibiting category of Sthapylococcus aureus bacteria, but in the chloroform fraction and ethanol extract can inhibit Escerichia coli bacteria categorized as strong. With it can be conlueded that the extract and fraction of soft Sinularia sp. have bioactive compounds with a broth spectrum of antibacterial activity.Keywords: Sinularia sp., Chloramphenicol, Escerichia coli, Sthaphylococcus aureus, and antibacterial. ABSTRAK Karang lunak Sinularia sp. telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri pada organisme biota laut yang diperoleh dari teluk Manado. Metode dalam penelitian ini adalah metode difusi agar dengan kontrol positif kloramfenikol dan kontrol negatit metanol pada Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak, fraksi kloroform, dan fraksi metanol-air dapat menghambat bakteri Staphylococcus aereus dikategorikan sedang, namun pada fraksi kloroform dan ekstrak etanol dapat menghambat bakteri Escerichia coli dikategorikan kuat. Dengan demikian dapat disimpulkan bahwa ekstrak dan fraksi karang lunak Sinularia sp.memiliki senyawa bioaktif dengan spektrum yang luas terhadap aktivitas antibakteri.Kata kunci: Sinularia sp, Kloramfenikol, Escherichia coli, Staphylococcus aureus, dan antibakteri

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals UJI AKTIVITAS ANTIBAKTERI JAMUR ENDOFIT AKAR BAKAU Avicennia marina TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Firdy Liwang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Avicennia Marina ◽  
Positive Control ◽  
Potato Dextrose Agar ◽  
Fungal Mycelium ◽  
Tidal Zone ◽  
E Coli

Abstract: In this his study we used endophytic fungi isolated from the roots of mangrove Avicennia marina growing on tidal zone around Tasik Ria Minahasa, North Sulawesi. The fungi were isolated and then tested the antibacterial effect against Staphylococcus aureus and Escherichia coli. Potato Dextrose agar was used in order to isolate the target fungi. The fungi began to grow on the second day after inoculation. Differentiation and purification processes to isolate the fungus obtained by observing fungi mycelia. While, the antibacterial activity test was following agar diffusion assay by planting 10 mm diameter of fungal mycelium together with particular bacteria onto the combination media. Ciprofloxacin was used as a positive control. The results shows a visible bacterial inhibition zone around the growing fungi with a diameter between 18 mm to 20.4 mm. Based on the results, it can be concluded that endophytic fungi isolated from the roots of mangrove Avicennia marina is able to inhibit the growth of S.aureus and E. coli. Keyword: Avicennia marina, Antibacterial, endophytic fungi   Abstrak: Penelitian ini menggunakan jamur endofit yang diisolasi dari akar tumbuhan bakau Avicennia marina yang tumbuh di perairan sekitar Tasik Ria Minahasa, Sulawesi Utara. Jamur endofit yang diisolasi kemudian diuji efek antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Isolasi jamur endofit menggunakan media PDA (Potato Dextrose Agar). Jamur mulai tumbuh pada hari kedua setelah inokulasi. Proses pemurnian jamur diperoleh dengan mengisolasi jamur dengan membedakan miselia. Pengujian efek antibakteri mengikuti prosedur agar difusi dengan menanam miselium jamur sebesar 10 mm pada media kombinasi yang telah diolesi bakteri uji. Sebagai kontrol positif digunakan Ciprofloxacin. Hasil penelitian terlihat diameter zona hambat pertumbuhan bakteri yang terbentuk sebesar 18 mm hingga 20,4 mm. Berdasarkan hasil penelitian maka dapat disimpulkan jamur endofit yang diisolasi dari akar tumbuhan bakau Avicennia marina mampu menghambat pertumbuhan bakteri S. aureus dan bakteri E. coli. Kata kunci: Avicennia marina, antibakteri, jamur endofit

Microwave Assisted Synthesis and Antibacterial Evaluation of 1, 3, 4-Thiadiazole Derivatives

2021 ◽  
pp. 5293-5296
Author(s):  
Jayalakshmi P M ◽  
Sheeba Jasmin TS ◽  
Manu Jose
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
New Drugs ◽  
Diffusion Method ◽  
Microwave Assisted Synthesis ◽  
Ir And Nmr Spectroscopy ◽  
Analgesic Agents ◽  
Ft Ir ◽  
And Staphylococcus Aureus ◽  
Heterocyclic Moiety

1,3,4-Thiadiazole is an important heterocyclic moiety, forms an integral core structural component of different categories of drugs such as antimicrobial, antitubercular, anti-inflammatory, antiepileptic, antiviral, antineoplastics, and analgesic agents. It is a key moiety in current discovery and designing of new drugs. The compounds were synthesised by both conventional method and microwave method. The targeted derivatives can be synthesised in a shorter time under microwave condition than under conventional reaction condition. Their structures were confirmed by FT-IR and NMR Spectroscopy. Antibacterial property of two synthesised analogs were evaluated by Agar well diffusion method against Escherichia coli and Staphylococcus aureus. The results of antibacterial activity showed that both the compounds were active against Staphylococcus aureus and inactive against Escherichia coli. Results of invitro studies showed that modifications in SB-2-PHB and SB-8-PHB will make it as a promising lead molecule for further research.

IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)

2017 ◽  
Vol 10 (4) ◽  
pp. 283
Author(s):  
Shirly Kumala ◽  
Ng Vini Aprilia ◽  
Partomuan Simanjuntak
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Fatty Acid ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Diffusion Method

Objective: Colletotrichium capsici endophytic fungi isolated from Jamblang plant (Eugenia cumini  L).  The secondary metabolites of this plant has the potent antibacterial efficacy  as well as diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli were fermented in Potato Dextrose Yeast (PDY) to produce  large scale of the metabolites.  Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3, n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.Results:  Antibacterial  test  results  indicated that fraction III had  the antibacterial  activity Staphylococcus aureus with  inhibition zone diameter  of 10.7 mm but no observed antibacterial activity against Escherichia coli.  Furthermore, identification  by GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.Conclusion:  In conclusion, secondary metabolites isolated from Jamblang plants branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity. Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity

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