scholarly journals Antioxidant and Antibacterial Activity of Plectaranthus amboinicus Leaf Extract

2019 ◽  
Vol 17 (2) ◽  
pp. 10
Author(s):  
Deri Islami ◽  
Hilwan Yuda Teruna ◽  
Yum Eryanti
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Herbal Medicine ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Perennial Plant ◽  
Ic50 Value ◽  
Free Radical Scavenging Assay ◽  
Agar Well Diffusion Assay

Plectranthus amboinicus (Lour.) is a perennial plant belonging to the family of Lamiaceae which have been widely used in traditional herbal medicine. In the present study, the extracts of the leaves of P. amboinicus that was extracted by different solvents were evaluated for their antioxidant and antibacterial properties. The fresh leaves of the plant were extracted using subsequent methanol, n-hexane and dichloromethane. The antioxidant activity was determined by DPPH free radical scavenging assay and the antimicrobial activity was tested against Escherichia coli and Staphylococcus aureus by agar well diffusion assay. The results of antioxidant activity test showed that dichloromethane extract of the leaves had the highest antioxidant activity among all with  IC50 value of 50.2 μg/mL Methanol and n-hexane extracts were still active in DPPH with IC50 value of 71.3 μg/mL and 205.9 μg/mL, respectively. Furthermore, the dichloromethane and methanol ektracts have shown a promising antibacterial activity in E. coli. The findings indicated that dichloromethane and methanol extracts of the leaves of P. amboinicus were potential further study as herbal medicine as they showed high antioxidant and antibacterial activities.

scholarly journals ANTIMICROBIAL, ANTIRADICAL AND INSECTICIDAL ACTIVITY OF GARDENIA GUMMIFERA L. F. (RUBIACEAE)

2017 ◽  
Vol 9 (10) ◽  
pp. 265 ◽  
Author(s):  
Prashith Kekuda T. R. ◽  
Raghavendra H. L. ◽  
Shilpa M. ◽  
Pushpavathi D. ◽  
Tejaswini Petkar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Insecticidal Activity ◽  
Leaf Extract ◽  
Antiradical Activity ◽  
Radical Scavenging ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Ic50 Value ◽  
Agar Well Diffusion Assay ◽  
Dose Dependent

Objective: The present study was carried out to investigate antimicrobial, antiradical and insecticidal potential of leaf and fruit of Gardenia gummifera L. f. (Rubiaceae).Methods: The leaf and fruits were shade dried, powdered and extracted by maceration process using methanol. Antibacterial activity was evaluated against Gram positive and Gram negative bacteria by Agar well diffusion assay. Antifungal activity was determined against six seed-borne fungi by Poisoned food technique. Antiradical activity of leaf and fruit extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) radical scavenging assays. Insecticidal activity of leaf and fruit extracts, in terms of larvicidal and pupicidal activity, was assessed against larvae and pupae of Aedes aegypti.Results: Both the extracts inhibited all test bacteria. Marked antibacterial activity was displayed by fruit extract when compared to leaf extract. S. epidermidis and E. coli were inhibited to highest and least extent by both extracts respectively. Fruit extract was found to exhibit higher antifungal effect when compared to leaf extract. Leaf extract and fruit extract exhibited highest inhibitory activity against A. niger and A. flavus respectively. Leaf and fruit extracts scavenged DPPH radical’s dose dependently with an IC50 value of 49.01µg/ml and 2.53µg/ml respectively. The scavenging of ABTS by leaf and fruit extracts was dose dependent and the IC50 value for leaf and fruit extract was 2.58µg/ml and 2.31µg/ml respectively. Fruit extract was shown to exhibit marked antiradical activity when compared to leaf extract. Leaf and fruit extracts exhibited dose dependent insecticidal activity in terms of larvicidal and pupicidal activity and the susceptibility of larvae and pupae to extracts was in the order II instar larvae>IV instar larvae>pupae. Fruit extract displayed marked insecticidal potential when compared to leaf extract.Conclusion: Overall, fruit extract of G. gummifera exhibited marked antimicrobial, antiradical and insecticidal activity when compared to leaf extract. The plant can be used for developing agents/formulations effective against infectious microorganisms, oxidative stress and insect vectors that transmit dreadful diseases. The observed bioactivities could be ascribed to the presence of active principles which are to be isolated and characterized.

EVALUATION OF ANTIOXIDANT ACTIVITY OF UNRIPE AEGLE MARMELOS CORR. FRUITS

2018 ◽  
pp. 4-7
Author(s):  
Gupta D ◽  
John PP ◽  
Kumar P ◽  
Jain J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Aegle Marmelos ◽  
Indian Origin ◽  
Ic50 Value ◽  
Phytochemical Compounds ◽  
Free Radical Scavenging Assay

Aegle marmelos Corr. (Rutaceae), commonly known as Bael, is a tree of Indian origin, well known from ancient period andprescribed for various ailments in Ayurveda. Utilization of bael fruit in day-to-day life has a great nutritional, environmental as well ascommercial importance. Every part of Aegle marmelos including stem, bark, root, leaves, fruit and seeds at all stages of maturity possesmedicinal virtues and has been used in Ethno medicine to exploit its medicinal properties. Objective: This study was undertaken to examine theantioxidant activity of methanolic extract of Aegle marmelos unripe or half ripe fruits. Material and Methods: The antioxidant activity was doneby using DPPH free radical scavenging assay. The IC50 (The concentration of sample required to scavenge 50% of DPPH free radical) wascalculated by plotting graph between % inhibition vs concentration. The ascorbic acid was used as standard antioxidant. Result and Discussion:The IC50 value of extract and ascorbic acid was found to be 62.59μg/ml and 2.80μg/ml. The antioxidant activity found in Aegle marmelos maybe associated with their main phytochemical compounds like flavonoids, phenols and tannins. Conclusion: This activity supports that the fruitcan be used as natural antioxidant to treat free radical induced cellular damages and can also be used as adjuvant with other drugs to givesynergistic effects.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

2021 ◽  
Vol 3 (2) ◽  
pp. 53-61
Author(s):  
Angga Crystal Loasana Yami ◽  
Irmanida Batubara ◽  
Kholis Abdurachim Audah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Ic50 Value ◽  
Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

scholarly journals Chemical composition, antioxidant and antibacterial activities of the essential oils of Juniperus phoenicea, Juniperus thurifera and Juniperus oxycedrus

2019 ◽  
Vol 9 (3) ◽  
pp. 190-198
Author(s):  
Houda El Hajjouji ◽  
Rachid Rahhal ◽  
Said Gmouh ◽  
Mohammed Hsaine ◽  
Hassan Fougrach ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Juniperus Thurifera ◽  
Juniperus Oxycedrus ◽  
Juniperus Phoenicea

This study aimed to compare the chemical composition, the antioxidant activity and the antibacterial activity of essential oils (EOs) of Juniperus phoenicea, Juniperus thurifera and Juniperus oxycedrus, obtained from Ait Bouguemez region (Province of Azilal, Morocco). The analysis by GC/MS of essential oils led to identify 37, 54 and 38 components for J. phoenicea, J. thurifera, and J. oxycedrus, respectively. Monoterpenic fraction was found predominant in essential oils of the three samples. The DPPH free radical scavenging activity showed that essential oil of J. thurifera has the strongest antioxidant activity with an IC50 value of 12.07 μg/mL. The antibacterial activity showed that S. aureus was more sensitive than P. aeruginosa and E. coli for the three EOs tested.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEUCAS ASPERA FLOWERS FROM BIHAR, INDIA.

2018 ◽  
Vol 11 (2) ◽  
pp. 223
Author(s):  
Gulnaaz Sabri ◽  
Vimala Y
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Free Radical ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Free Radical Scavenging Assay

 Objective: The aim of this study was to explicate antibacterial, antifungal, and antioxidant activities of Leucas aspera flowers.Methods: Antibacterial activity was done by agar diffusion method. The ethyl acetate extract of L. aspera flower was evaluated against both Gram-positive and Gram-negative bacteria. Antifungal activity was also done by agar diffusion method. The agar used for antifungal activity was Czapek Dox Agar. Nitric oxide scavenging assay and free radical scavenging assay were used for the antioxidant activity. Griess reagent was used in nitric oxide scavenging assay. 1,1-diphenyl-2-picryl hydrazyl was used in free radical scavenging assay.Results: L. aspera flower extract showed good antibacterial activity with the highest zone of inhibition against Vibrio cholera with 23 mm followed by Bacillus polymyxa showing 20 mm zone of inhibition. The ethyl acetate extract of L. aspera flower showed quite a good results with the highest inhibitory activity against Aspergillus niger with 13 mm zone of inhibition and lowest for Trichoderma viridae with 5 mm zone of inhibition. Antioxidant activity of L. aspera flower extract was done by free radical scavenging assay and nitric oxide scavenging assay. Nitric oxide scavenging assay showed prominent results almost performed equal to standard compound Butylated hydroxyl anisole (BHA) The values for 10 μl of L. aspera extract was 50.27, for the standard (BHA) showed 50.81. L. aspera extract values for 50 μl was 69.73 and for BHA, the values was 77.30. For 100 μl, the extract gave 82.70, and for standard BHA, the reading was 89.73.Conclusion: The results showed that L. aspera flower has broad-spectrum antibacterial activity ranging from 23 to 13 mm zone of inhibition. L. aspera flower has strong antioxidative power on nitric oxide radicals. The medicinal properties of plant species have made an outstanding contribution to the origin and evolution of many traditional herbal therapies.

Synthesis, antioxidant and antibacterial activity of azo dye-stilbene hybrid compounds

2019 ◽  
Vol 48 (1) ◽  
pp. 84-88 ◽  
Author(s):  
Esmail Rezaei-Seresht ◽  
Aboulfazl Salimi ◽  
Behnam Mahdavi
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Azo Dyes ◽  
Radical Scavenging ◽  
Coupling Reaction ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Butylated Hydroxytoluene ◽  
Azo Compounds ◽  
Content Type

Purpose The purpose of this paper is synthesis and evaluation of antioxidant and antibacterial activities of a series of new azo dyes derived from 4-aminostilbene. Design/methodology/approach First, the starting material 4-aminostilbene was prepared via two successive Wittig and reduction reactions from 4-nitrobenzyl bromide. The obtained 4-aminostilbene was then reacted with some phenols under the normal azo coupling reaction conditions to give five new azo products. Antioxidant activity of the azo compounds was determined by radical scavenging assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Also, the antimicrobial activity of the compounds against one gram-positive and eight gram-negative strains was evaluated based on the inhibition zone using disc diffusion assay. Findings The structures of the azo dyes were identified and characterized by fourier-transform Infrared, 1H nuclear magnetic resonance (NMR) and ultraviolet-visible (UV-V) is spectroscopic methods. All the compounds showed higher antioxidant activity than ascorbic acid (Asc) and butylated hydroxytoluene (BHT) as positive controls. Moreover, the compounds showed lower antibacterial activity than the standard antibiotic vancomycin. Research limitations/implications Excellent antioxidant activity, along with antibacterial activity against Streptococcus pneumoniae and Pseudomonas aeruginosa, was observed for the two synthesized azo dyes. Originality/value Five novel azo dyes based on 4-aminostilbene were synthesized. The dyes have a highly p-extended conjugated structure comprising the phenolic and stilbenic segments, and they indicated good antioxidant activity, so that the two dyes (2c and 2d) even showed much more scavenging activity compared to BHT which is used as an antioxidant agent in food industries. These compounds with highest antioxidant activity also inhibited the growth of S. pneumoniae and P. aeruginosa.

scholarly journals Evaluation of the Antibacterial and Antioxidant Activity of Mimosa rubicaulis and Reinwardtia indica

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Roshani Gurung ◽  
Sundar Adhikari ◽  
Kalpana Parajuli
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Antibacterial Activity ◽  
Plant Species ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Ic50 Value ◽  
Antibacterial And Antioxidant Activity

Many plants have the property of wound healing, but most of the people are using costly allopathic medicine for the wound. This might be due to lack of awareness about the traditional uses and lack of scientific study of ethnomedicinal plants. So, this study aimed to carry out the antibacterial and antioxidant activity of two medicinal plants which are used traditionally for wound healing activity, i.e.,Mimosa rubicaulis and Reinwardtia indica. Different parts of Mimosa rubicaulis (root, stem, and leaves) and Reinwardtia indica (flower and leaves) were used for the study. Extractions were done by maceration using ethanol as solvent. Antibacterial activity was carried out by the well diffusion method, and antioxidant activities were screened by DPPH radical scavenging and NO scavenging assays. Extract of M. rubicaulis has shown a weak zone of inhibition towards S. aureus and P. vulgaris whereas R. indica has shown no zone of inhibition towards selected bacterial strains. Leaf, root, and stem extracts of M. rubicaulis have shown potent antioxidant activity, i.e., IC50 value of 9.8 µg/ml, 10.19 µg/ml, and, 13.64 µg/ml, respectively. Similarly, leaf extract of R. indica exhibited antioxidant activity with an IC50 value of 19.73 μg/ml. The percentage inhibition of NO radicals of root and stem of M. rubicaulis and leaves of R. indica was 31%, 21%, and 22%, respectively. Out of these two plant species, M. rubicaulis had shown antibacterial activity towards selected microorganism, but antioxidant activity was shown by both plant species. These properties on above mentioned two plant species might help for the development of a marketed formulation for antibacterial and wound-healing agent since wound healing is promoted by antibacterial and antioxidant activities.

scholarly journals Phytochemical Content, Antioxidant, Alpha-Glucosidase Inhibitory and Antibacterial Activities of Spineless Cactus Pear Cultivars

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1312
Author(s):  
Mologadi B. Mabotja ◽  
Sonja L. Venter ◽  
Christian P. Du Plooy ◽  
Tukayi Kudanga ◽  
Stephen O. Amoo
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Cactus Pear ◽  
Phytochemical Content ◽  
E Coli ◽  
Alpha Glucosidase ◽  
Phenolic And Flavonoid

Variation in cultivars can influence plant biological activities. This study aimed to identify superior cultivars while determining the variability in the phytochemical content, antioxidant, alpha-glucosidase inhibitory and antibacterial activities of cladode extracts from selected spineless Burbank cactus pear (Opuntia ficus-indica and Opuntia robusta) cultivars. Total phenolic and flavonoid contents were determined using the Folin-Ciocalteu and aluminum chloride spectrophotometric methods, respectively. Antioxidant activity was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene linoleic acid assays. Alpha-glucosidase inhibition was determined using a spectrophotometric method and antibacterial activity using a non-polar (petroleum ether) and polar (50% methanol) extracts against two Gram-positive and two Gram-negative bacteria. Significant variation in phytochemical content, antioxidant, antidiabetic and antibacterial activities was observed amongst the cultivars. Alpha-glucosidase inhibitory activity varied widely with IC50 values ranging from 0.06 to 1.85 mg/mL. Radical scavenging activity of Polypoly cultivar was about seven fold higher than that recorded in other cultivars with low activity. Turpin and Berg x Mexican cultivars had the highest total phenolic and flavonoid contents, whilst the non-polar extract of Turpin also exhibited higher antibacterial activity against Bacillus subtilis and Escherichia coli. Sicilian Indian Fig was amongst the cultivars with a higher antioxidant activity, whilst also showing a strong inhibition against B. subtilis and E. coli. Polypoly cultivar demonstrated strong antioxidant and antidiabetic activities while its polar extract showed the highest total antibacterial activity against B. subtilis. The cultivar Malta was superior in terms of its antibacterial potency and efficacy against B. subtilis, Staphylococcus aureus and E. coli. The potential of using spineless cactus pear cladodes as a functional food with antioxidant, antidiabetic and antibacterial properties against pathogenic food spoilage bacteria in place of synthetic compounds was established. The significance of cultivar selection to increase this potential was highlighted.

scholarly journals Antioxidant and Antibacterial Activities of Four Local Medicinal Plants

2020 ◽  
Vol 30 (2) ◽  
pp. 179-187
Author(s):  
Barna Goswami ◽  
Shamoly Akter ◽  
Nemai Chandra Nandi ◽  
Tanjina Akthar Banu ◽  
Shahina Akter ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Leaf Extracts ◽  
Ic50 Value

Potential antioxidant and antibacterial activity of methanolic, chloroformic and n-hexane leaf extracts of four local important medicinal plants like Ocimum americanum, O. basilicum, O. gratissimum and Centella asiatica was investigated. The methanolic leaf extracts of these plant species exhibited the potent DPPH free radical scavenging activity (IC50 value, 2.67 ± 0.01, 14.17 ± 0.11, 60.22 ± 0.01 and 2.39 ± 0.025 μg/ml, respectively). Methanolic leaf extract of C. asiatica showed strongest antioxidant activity. Chloroformic leaf extracts possessed moderate antioxidant activity (IC50 value of 79.44 ± 0.05, 110.56 ± 0.02, 54.95 ± 0.05, 101.0 ± 1.0 μg/ml, respectively) in all samples. The lowest antioxidant activity was recorded from n-hexane leaf extracts of O. americanum, O. gratissimum, C. asiatica and Ocimum basilicum (IC50 value 147.87 ± 0.06, 378.19 ± 2.65, 104.65 ± 0.39, 467.58 ± 0.52 μg/ml, respectively). Methanolic and chloro-formic leaf extracts showed antibacterial activity against both Gram-positive and Gram-negative pathogenic bacteria, namely Bacillus megaterium, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi. Methanolic leaf extract of O. americanum and chloroformic extract of C. asiatica showed excellent antimicrobial activity. Plant Tissue Cult. & Biotech. 30(2): 179-187, 2020 (December)

A Facile Eco-Friendly One-Pot Five-Component Synthesis of Novel 1,2,3-Triazole-Linked Pentasubstituted 1,4-Dihydropyridines and their Biological and Photophysical Studies

2013 ◽  
Vol 66 (9) ◽  
pp. 1088 ◽  
Author(s):  
Harjinder Singh ◽  
Jayant Sindhu ◽  
Jitender M. Khurana ◽  
Chetan Sharma ◽  
Kamal R. Aneja
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Photophysical Properties ◽  
Radical Scavenging ◽  
One Pot ◽  
Gram Positive Bacteria ◽  
Microbial Strains ◽  
Free Radical Scavenging Assay ◽  
New Compounds

An eco-friendly one-pot five-component synthesis of novel 1,2,3-triazole-linked pentasubstituted 1,4-dihydropyridines under ultrasonic and microwave irradiation in polyethylene glycol (PEG) 400 is described. All newly synthesised compounds were evaluated for antibacterial activity, antifungal activity, antioxidant activity, and photophysical properties. Antimicrobial activity was evaluated against six microbial strains. All compounds exhibited antifungal activity against Aspergillus niger and Aspergillus flavus and moderate antibacterial activity against Gram positive bacteria (Staphylococcus aureus and Bacillus subtilis). Antioxidant activity was evaluated using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. All compounds showed good to moderate antioxidant activity. Furthermore all new compounds showed strong fluorescence in solution.

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