scholarly journals Isolation of Flavonoids from Anaphalis Busua and their Antibacterial Activity

2020 ◽  
Vol 25 (1) ◽  
pp. 1-6
Author(s):  
M. Rajbhandari ◽  
U. Lindequist
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Inhibitory Concentration ◽  
Hexane Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Preparative Hplc ◽  
Direct Bioautography

Anaphalis busua (Buch-Ham ex D. Don) is traditionally used to treat cuts and wounds. In the agar dilution method, the hexane extract of the aerial part of A. busua exhibited potent antibacterial activity with a minimal inhibitory concentration of 25 μg/ml against Bacillus subtilis and 500 μg/ml against Staphylococcus aureus. For the isolation of antibacterial compounds, the hexane extract was fractionated by silica gel and Sephadex LH 20 column chromatography. The direct bioautography method was used to determine the antibacterial activity of the fractions. The active fractions were finally purified by semi-preparative HPLC on C18 Phenomenex column under gradient condition. Four flavones derivatives of 3,5-dihydroxy-6,7,8-trimethoxyflavone (1), 3,5,7-trihydroxy-6-methoxyflavone (alnusin) (2), 3,5,7-trihydroxy-8-methoxyflavone (3) and pinocembrin (4) were isolated, and their structures were established by 1H, 13C, DEPT-135, and ESI-MS spectroscopy. These compounds were isolated for the first time from A. busua. The fatty acid profile of the hexane extract was analyzed by gas chromatography-mass spectrometry (GC-MS) by silylation with N-methyl-N-trimethylsilyl-trifluoro-acetamide. Pinocembrin showed antibacterial activity with the minimum inhibitory concentration of 60 μg/mL against Bacillus subtilis and 420 μg/mL against Staphylococcus aureus.

scholarly journals Antibacterial Diterpenes from the Roots of Ceriops tagal

2008 ◽  
Vol 3 (1) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Musa Chacha ◽  
Renameditswe Mapitse ◽  
Anthony J. Afolayan ◽  
Runner R. T. Majinda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Spectroscopic Data ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Bacterial Strains ◽  
Agar Dilution ◽  
Ceriops Tagal

Investigation of the roots of Ceriops tagal led to the isolation of a new isopimarane, together with the known diterpenes isopimar-8(14)-en-15,16-diol and erythroxyl-4(17),15(16)-dien-3-one. The structure of the new compound was identified as isopimar-8(14)-en-16-hydroxy-15-one. These structures were determined from extensive spectroscopic data analysis. The isolates were screened for antibacterial activity using the agar dilution method against ten test bacterial strains ( Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Micrococcus kristinae, Pseudomonas aeruginosa, Salmonella pooni, Serratia marcescens, Staphylococcus aureus, S. epidermidis and Streptococcus pyrogens). Isopimar-8(14)-en-16-hydroxy-15-one exhibited activity, with MIC values of 0.5 mg/mL against Streptococcus pyrogens; 0.25 mg/mL against Salmonella pooni and 0.1 mg/mL against Bacillus cereus, Staphylococcus aureus and Micrococcus kristinae.

scholarly journals PROFIL KANDUNGAN KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN BAMBAN (Donax canniformis (G. Forst.) K. Schum.) TERHADAP Staphylococcus aureus

2015 ◽  
Vol 1 (2) ◽  
pp. 141-148
Author(s):  
Hidayatullah Hidayatullah ◽  
Syariful Anam ◽  
Muhamad Rinaldhi Tandah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Minimum Bactericidal Concentration ◽  
Dilution Method ◽  
The Family

Bamban (Donax canniformis (G. Forst.) K. Schum.) is one of the family Marantaceae plant that has many uses such as traditional medicine. Methanol extract of bamban leaves contains phenolic, tannins and saponins compounds. The purpose of this research is to determine the class of compounds that has antibacterial activity against Staphylococcus aureus and determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methanol extract of bamban leaves. This extract was prepared using maceration method with methanol solvent. Determination the class of compounds was initiated by bioautografi test in order to determine spots which has have antibacterial activity. Subsequently, the spot were identified the class of compound using reagent spray FeCl3 and H2SO4 10%. The determination of MIC and MBC using dilution method. Research showed there are three compounds that had antibacterial activity. These compounds were predicted as spot I and spot II which were phenolic compounds and spot III as a saponin compound. MIC and MBC value of the methanol extract of leaves bamban leaves 8% and 13%, respectively.

scholarly journals Antibacterial Activity of Murrayaquinone A and 6-Methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Biswanath Chakraborty ◽  
Suchandra Chakraborty ◽  
Chandan Saha
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Naturally Occurring

The antibacterial activity of Murrayaquinone A (10), a naturally occurring carbazoloquinone alkaloid, and 6-methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione (11), a synthetic carbazoloquinone, both obtained during the development of the synthesis of Carbazomycin G, having unique quinone moiety, was studied against Gram-positive (Bacillus subtilisandStaphylococcus aureus) and Gram-negative (Escherichia coliandPseudomonassp.) bacteria. Compound10showed antibacterial activities against both ofEscherichia coliandStaphylococcus aureuswhereas compound11indicated the activity againstStaphylococcus aureusonly. Both compounds10and11exhibited minimum inhibitory concentration (MIC) of 50 μg mL−1againstStaphylococcus aureus.

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI KRIM EKSTRAK ETANOL HERBA SELEDRI (Apium graveolens L.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 226
Author(s):  
Garry Clements ◽  
Paulina V. Y. Yamlean ◽  
Widya Astuty Lolo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Apium Graveolens ◽  
Dilution Method ◽  
Herb Extract

ABSTRACTCelery herbs (Apium graveolens L.) have content flavonoid, saponin and tannin. Flavonoids have anti-inflammatory properties, antioxidants and antibacterials. This study aims to determine whether the ethanol extract of celery herbs can be formulated into cream preparations, test the quality of preparations at concentrations of 5%, 10% and 15%. These methods of research are experimental laboratories by making the supply of self-sustaining herbal celery ethanol extract cream and testing the quality of the cream preparation,  as well as testing of antibacterial activity was carried out using the liquid dilution method and measuring value of  Minimum Inhibitory Concentration  and Minimum Bactericidal Concentration using a UV-Vis Spectrophotometer. This research shows that celery herbs ethanol extract can be formulated into cream preparations, fulfilling quality test of organoleptic, scatter power, adhesion and cycling test, but that doesn’t meet the requirements for homogeneity tests and pH tests and has antibacterial activity which not only inhibits but kills bacteria in preparations of 5% and 15%Keywords: Cream Celery herb extract, Antibacterial, Staphylococcus aureus, UV-Vis spectrophotometer ABSTRAK Herba Seledri (Apium graveolens L.) memiliki kandungan flavonoid, saponin dan tannin. Flavonoid memiliki khasiat sebagai antiinflamasi, antioksidan dan antibakteri. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, menguji mutu sediaan pada konsentrasi 5%, 10% dan 15%. Metode penelitian ini experimental laboratorium dengan membuat Sediaan Krim Ekstrak Etanol Herba Seledri dan menguji mutu sediaan krim, serta   menguji aktivitas antibakteri menggunakan metode dilusi cair dan mengukur nilai KBM dan KHM menggunakan Spektrofotometer UV-Vis. Hasil penelitian menujukkan bahwa ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, memenuhi uji mutu sediaan organoleptik, daya, sebar, daya hambat dan stabilitas  namun tidak memenuhi persyaratan untuk uji homogenitas dan uji pH serta mempunyai aktivitas antibakteri yang membunuh bakteri pada sediaan berkonsentrasi 5% dan 15%.Kata Kunci : Krim Ekstrak herba Seledri, Antibakteri, Staphylococcus aureus, Spektrofotometer UV-Vis

scholarly journals Uji aktivitas antibakteri ekstrak biji petai cina (Leucaena leucocephala) terhadap Staphylococcus aureus

2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Vivi Permata Sari ◽  
Wiwin Retnowati ◽  
Yuani Setiawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimal Inhibitory Concentration ◽  
Leucaena Leucocephala ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Seed Extract ◽  
Dilution Method ◽  
Minimal Bactericidal Concentration ◽  
Vancomycin Resistant

 Abstract. Staphylococcus aureus merupakan flora normal kulit manusia yang bisa menyebabkan infeksi ketika sistem imun tubuh manusia sedang lemah. Pengobatan Staphylococcus aureus tergolong sulit karena Staphylococcus aureus mudah resisten terhadap beberapa antibiotik sehingga dikenal istilah Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-intermediate Staphylococcus aureus (VISA), dan Vancomycin-resistant Staphylococcus aureus (VRSA) yang menunjukkan resistensi tersebut. Pertumbuhan Staphylococcus aureus dapat dihambat ekstrak daun petai cina (Leucaena leucocephala). Tanaman petai cina (Leucaena leucocephala) digunakan masyarakat Indonesia sebagai obat tradisional untuk menyembuhkan luka, mengobati cacingan, dan mengobati jerawat. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak biji petai cina (Leucaena leucocephala) dalam menghambat pertumbuhan Staphylococcus aureus.Penelitian ini merupakan eksperimental laboratorium dengan metode dilusi sebanyak 6 replikasi. Setiap replikasi menggunakan konsentrasi ekstrak biji petai cina (Leucaena leucocephala) sebesar 3,125%, 6,25%, 12,5%, 25%, 50%, dan 100% untuk mencari konsentrasi hambat minimal dan konsentrasi bunuh minimal terhadap Staphylococcus aureus.Hasil penelitian tidak dapat menentukan konsentrasi hambat minimal (KHM) ekstrak biji petai cina (Leucaena leucocephala) terhadap Staphylococcus aureus karena warna ekstrak yang gelap. Sedangkan konsentrasi bunuh minimal (KBM) ekstrak biji petai cina (Leucaena leucocephala) terhadap Staphylococcus aureus ditemukan pada konsentrasi 50%, yang menunjukkan bahwa konsentrasi 50% adalah konsentrasi terkecil dimana tidak terdapat pertumbuhan bakteri Staphylococcus aureus.Sebagai simpulan, ekstrak biji petai cina (Leucaena leucocephala) memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Kata kunci: Staphylococcus aureus, Leucaena leucocephala, metode dilusi  Abstract. Staphylococcus aureus is a normal human skin flora that can cause infection when human immune system is weak. Staphylococcus aureus treatment is difficult because Staphylococcus aureus is easily resistant to some antibiotics so several terms such as Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-intermediate Staphylococcus aureus (VISA), and Vancomycin-resistant Staphylococcus aureus (VRSA) are known to indicate the resistance. The growth of Staphylococcus aureus can be inhibited by Leucaena leucocephala leaf extract. Leucaena leucocephala is used by Indonesian people as traditional medicine to heal wounds, treat worm infection, and treat acne. The objective of this study was to identify the antibacterial activity of Leucaena leucocephala seed extract against Staphylococcus aureus.This was a laboratory experimental study using dilution method as many as five replications. Each replication used Leucaena leucocephala seed extract in concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100% to determine the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) against Staphylococcus aureus. The results were not able to determine the minimal inhibitory concentration (MIC) of Leucaena leucocephala seed extract against Staphylococcus aureus due to dark color of the extract. While the minimal bactericidal concentration (MBC) of Leucaena leucocephala seed extract against Staphylococcus aureus was 50%. This showed that the concentration of 50% was the lowest concentration where there was no growth of the bacteria Staphylococcus aureus.In conclusion, the extract of Leucaena leucocephala seed has antibacterial activity against Staphylococcus aureus. Keywords: Staphylococcus aureus, Leucaena leucocephala, dilution method

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BUAH MAHKOTA DEWA (Phaleria macrocarpa) TERHADAP STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 9 (02) ◽  
pp. 134-139
Author(s):  
Yunilda Rosa ◽  
Masnir Alwi ◽  
Monica Julianti
Keyword(s):  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dilution Method ◽  
Phaleria Macrocarpa

Phaleria macrocarpa is one of the medicinal plants that many people use as a traditional medicinal which has many benefits, one of which is as a medicine for skin diseases. The content of flavonoid compounds in Phaleria macrocarpa believed to have potential as an antibacterial. The purpose of this study was to know the antibacterial activity of the ethanol extract of the Phaleria macrocarpa to inhibized Staphylococcus aureus bacteria. This research is an experimental study carried out by the liquid dilution method (KHM)and disk diffusion (KBM) of the extract of the Phaleria macrocarpa is divided into 6 concentrations namely 5%, 10% and 15% (KHM) and 40%, 50% and 60% (KBM). The positive control used is clindamycin and the negative control is aquadest. Data analysis uses univariate. Based on observations of the minimum inhibitory concentration of the extract of the fruit of the Phaleria macrocarpa which is at of 15%, while the minimum killed concentration is formed at of 40% with an average of 6.36 mm, and an average of zones formed on the positive control of 9.53 mm. From the results of the study, it was concluded that the extract of the fruit of the Phaleria macrocarpa can inhibit and kill the growth of Staphylococcus aureus bacteria.

scholarly journals Antibacterial Activity Of Methanol Extract Of Meranti Sarang Punai Cortex (Shorea parvifolia Dyer) Against Staphylococcus aureus And Propionibacterium acnes

2020 ◽  
pp. 160
Author(s):  
Vilya Syafriana ◽  
Tiah Rachmatiah ◽  
Nitya Wita Utama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Woody Plant ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Methanol Extract ◽  
Extraction Methods ◽  
Diffusion Method ◽  
Dilution Method ◽  
Disc Diffusion Method

Shorea parvifolia Dyer (meranti sarang punai) is one of a woody plant in Indonesia which distributed across Sumatra and Kalimantan. Cortex of meranti sarang punai is known contains the secondary metabolite such as saponin, flavonoid, and tannin which can act as an antibacterial agent. The research was aims to know the antibacterial activity of methanol extract of meranti sarang punai against Staphylococcus aureus and Propionibacterium bacteria. Maceration was chosen as the extraction methods with methanol as a solvent. The antibacterial test was done by disc diffusion method in Nutrient Agar (NA) media with concentrations 5%, 10%, 15%, 20%, 25%, and 30%; while the Minimum Inhibitory Concentration (MIC) was done by solid dilution method with concentrations 5%, 4%, 3%, 2%, and 1%. The results showed that the methanol extract of meranti sarang punai has antibacterial activity against the S. aureus with Inhibitory Zone (IZ) at concentrations of 5%, 10%, 15%, 20%, 25%, and 30% respectively 16,68; 17,27; 18,41; 19,4; 20,57; and 21,46 mm, while against P. acnes was 12,61; 13,65; 14,57; 15,53; 16,58; and 17,46 mm. The MIC of methanol extracts of meranti sarang punai against the two bacteria was at concentration 2%.

scholarly journals Phytochemical Evaluation and Antibacterial Activity of Fruit Extract of Solanum surattense Burm F. against Some Pathogenic Bacteria

2020 ◽  
Vol 13 (1) ◽  
pp. 79-87
Author(s):  
R Dhanalakshmi ◽  
J JaffarIqbal ◽  
Nausath Jabeen K ◽  
Noorul Zaithun Hidhaya M ◽  
K Meena ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
Hydroalcoholic Extract ◽  
Solanum Surattense

The objective of this study was to evaluate phytochemical constituents and antibacterial activity of hydroalcoholic extract of Solanum surattense fruit against some grampositive and gram-negative bacterial strains. The evaluation of antibacterial activity was carried out by using the disc diffusion method, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Ciprofloxacin was used as positive control. Hydroalcoholic extract of Solanum surattense fruit containing alkaloids, flavonoids, phenol, saponins, terpenoids, glycosides, sterols, proteins and tannins. Efficacy data analysis showed that hydroalcoholic extracts of fruit of Solanum surattense (1 mg/ml) inhibited the growth of Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi with mean diameters of inhibition zones being 24, 25, 28 and 30 mm respectively. On the other hand, minimum inhibitory concentration and minimum bactericidal concentration value of 0.062 and 0.25 mg/ml, 0.062 and 0.25 mg/ml, 0.312 and 0.125 mg/ml, 0.156 and 0.0312 mg/ml were recorded against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi respectively. Hydroalcoholic extracts of Solanum surattense fruit have potent antibacterial activity against the different tested bacterial strains. This activity supports their use in treatment of infections caused by such resistant bacteria.

scholarly journals ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF JAVANESE TURMERIC RHIZOME ENTRAPPTED IN NANOPARTICLES: A NOVEL RULE OF CHITOSAN

2018 ◽  
Vol 10 (6) ◽  
pp. 298
Author(s):  
Deni Rahmat ◽  
Shirly Kumala ◽  
Yunahara Farida ◽  
Benny Chandra
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Particle Size ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Zeta Potential ◽  
Chitosan Nanoparticles ◽  
Ethanolic Extract ◽  
Dilution Method ◽  
Broth Dilution

Objective: The aim of this study was to compare the antibacterial activity of 96% (v/v) ethanolic extract of Javanese turmeric rhizome (Curcuma xanthorrhiza Roxb.) and nanoparticles containing the extract against 4 pathogenic bacteria.Methods: The synthesis of chitosan nanoparticles used ionic gelation method. The nanoparticles were evaluated for particle size, zeta potential and morphology. Dilution method was performed to determine minimum inhibitory concentration (MIC).Results: The nanoparticles showed particle size of 53.25 nm, polydispersity index of 0.442 and zeta potential value of+31.5 mv. The antibacterial activity results based on the broth dilution method demonstrated that both the extract and the nanoparticles could inhibit the growth of Staphylococcus aureus, Escherichia coli, Salmonella thypi, and Bacillus subtilis in a concentration of 500 µg/ml. The MIC of the extract against Staphylococcus aureus and Bacillus subtilis were 400 µg/ml and against Escherichia coli and Salmonella thypi in a concentration of 500 µg/ml. In addition, the MIC of the nanoparticles against Staphylococcus aureus was 300 µg/ml while against Bacillus subtilis and Salmonella thypi 400 µg/ml and against Escherichia coli 500 µg/ml.Conclusion: The nanoparticles have a promising strategy to formulate the extracts in order to decrease the MIC against Staphylococcus aureus and Salmonella thypi.

scholarly journals Antibacterial Activity of Bandotan (Ageratum conyzoides L) Leaves Extracts Against Methicillin-Resistant Staphylococcus aureus

2020 ◽  
Vol 3 (4) ◽  
pp. 243-248
Author(s):  
Selvira Anandia Intan Maulidya ◽  
Doni Anshar Nuari ◽  
Shendi Suryana ◽  
Sumia Almarifah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanolic Extract ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Ageratum Conyzoides ◽  
Methicillin Resistant ◽  
Ethanolic Extracts ◽  
Mic Value

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections throughout the world and can be life-threatening as well. This study aimed to determine the antibacterial activity of Bandotan (Ageratum conyzoides L) leaves ethanolic extract against MRSA's growth. Ageratum conyzoides leaves were extracted by ethanol and screened for their phytochemical constituent. Ethanolic extracts of A. conyzoides leaves were evaluated for their potential antibacterial activity using disc diffusion assay. The minimum inhibitory concentration (MIC) value was determined using the agar dilution method. Phytochemical screening shows that the extracts contain alkaloids, flavonoids, saponins, tannins, and steroids or triterpenoids. Ageratum conyzoides leaves extract shows a 25.1 mm inhibitory zone at 12.5% extract concentration with MIC value equivalents to 4.46 x 10-6 g of gentamicin. This study concludes that A. conyzoides leaves ethanolic extracts have potential antibacterial activity against MRSA.

Sign in / Sign up

Export Citation Format

Share Document