scholarly journals Quantification of pterygium fibroconnective components utilizing freeze-dried method

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Mohd Radzi Hilmi ◽  
Khairidzan Mohd Kamal ◽  
Mohd Zulfaezal Che Azemin ◽  
Azrin Esmady Ariffin
Keyword(s):  
Vascular Tissue ◽  
Statistical Significance ◽  
Dry Weight ◽  
Test Results ◽  
Fixed Group ◽  
Freeze Dried ◽  
Wet Weight ◽  
The Difference ◽  
Formalin Fixed ◽  
Primary Pterygium

Introduction: To propose an objective method of quantifying pterygium fibroconnective components utilizing freeze-dried method. Methods: 60 primary pterygium were excised using controlled partial avulsion technique performed by a single surgeon (KMK), and divided into two groups: formalin-fixed (n=30) and fresh (n=30). Initially, each sterile container weight was measured and 5ml of 5% buffered formaldehyde were filled and stored for 1 week for formalin-fixed group while, 5ml distilled water were filled for fresh pterygium group. Each container was pre-frozen for 12 hours prior to freeze-dried (-80ºC for 24 hours). The final product known as fibroconnective tissue, then weighted as dry weight. Comparative analysis of wet and dry weight, and percentage of fibroconnective components between groups were performed via an independent t-test. Results: The overall mean and SD for formalin-fixed and fresh pterygium wet weight were 253.33 ± 82.17 μg and 255.17 ± 63.52 μg, and dry weight were 184.92 ± 84.31 μg and 179.54 ± 72.85 μg respectively. In terms of percentage of fibroconnective components, formalin-fixed group revealed slightly higher percentage compared to fresh pterygium tissue with 69.39 ± 13.29 % and 67.75 ± 13.27 % respectively. The difference in pterygium dry weight, between formalin-fixed and fresh pterygium tissue was statistically insignificant (p=0.792). Conclusions: The freeze-dried method can be used to quantify the fibroconnective component (dry weight) of pterygium fibro vascular tissue. Both methods (formalin-fixed and freeze-dried) are reliable in producing results. This is proven as there is no statistical significance between the two methods.

The Experimental Study on Cryofreeze-Drying of Porcine Aortic

2009 ◽  
Author(s):  
Jinfeng Wang ◽  
Leren Tao ◽  
Yonghong Wang ◽  
Lihao Huang ◽  
Shuhong Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tensile Stress ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Effective Means ◽  
Test Results ◽  
Fresh Blood ◽  
Vascular Morphology ◽  
Axial Tensile ◽  
Freeze Dried

At present, the vascular tissue disease has become the major cause to against people’s health, vascular graft is an effective means for the treatment of vascular tissue disease, but the preservation of blood vessels has always been a big challenge. This paper based on the conduct of porcining aortic cryofreeze-drying, with using micro-CT to track the whole drying process, expect to reveal what vascular morphology changes have taken place during the process of cryofreeze-drying, and take a comparative analysis of porcine aortic mechanical properties between freeze-dried after the restoration of water and fresh porcine aortic by the Texture analyzer, so that uncover the effect the process of freeze-dried making on pig arteries mechanical properties. Test results showed that the pig aorta vascular occur stratification in the process of cryofreeze-drying, blood vessels freeze-dried following the resumption of water comparing the mechanical properties with the fresh blood vessels, its maximum axial tensile stress reduced by about 40%, and the largest peripheral tensile stress increase by about 45%, and the biggest puncture stress is about 75 percent of fresh blood vessels.

Muscular enlargement and number of fibers in skeletal muscles of rats

1981 ◽  
Vol 50 (5) ◽  
pp. 936-943 ◽  
Author(s):  
P. D. Gollnick ◽  
B. F. Timson ◽  
R. L. Moore ◽  
M. Riedy
Keyword(s):  
Skeletal Muscles ◽  
Extensor Digitorum Longus ◽  
Dry Weight ◽  
Extensor Digitorum ◽  
Surgical Ablation ◽  
Fiber Number ◽  
Wet Weight ◽  
The Difference ◽  
Total Muscle ◽  
Direct Counts

The effect of muscular enlargement produced by surgical ablation of a synergist and the combination of synergist ablation and exercise on the number of fibers in the soleus (S), plantaris (P), and extensor digitorum longus (EDL) muscles of the rat was studied. The number of fibers per muscle was determined by direct counts of individual fibers dissected from HNO3-treated muscles. Ablation of a synergist produced average enlargements of about 25, 45, and 29% for the S, P, and EDL muscles, respectively. Exercise and synergist ablation produced increases in wet weight to about 44 and 88% for the S and P muscles, respectively, whereas no further increases were observed in the EDL muscles. Intra-animal comparisons revealed that no differences existed for total fiber number or the incidence of fibers with bifurcations between the enlarged and contralateral control muscles. The difference in dry weight of fibers from the enlarged as compared with control muscles was closely correlated to differences in total muscle wet weight. These data demonstrate that hypertrophy rather than hyperplasia was responsible for increases from 10 to over 100% in the weight of skeletal muscles.

scholarly journals Mercury concentration in bivalve molluscs

2015 ◽  
Vol 59 (3) ◽  
pp. 357-360 ◽  
Author(s):  
Józef Szkoda ◽  
Maciej Durkalec ◽  
Agnieszka Nawrocka ◽  
Mirosław Michalski
Keyword(s):  
Total Mercury ◽  
Analytical Procedure ◽  
Absorption Spectrometry ◽  
Dry Weight ◽  
Proficiency Tests ◽  
Bivalve Molluscs ◽  
Quality Assurance Programme ◽  
Freeze Dried ◽  
Wet Weight

AbstractA total of 85 mussel samples of eight species were examined. Analysis of mercury in the freeze-dried samples was carried out by atomic absorption spectrometry method using direct mercury analyser AMA 254. The analytical procedure for determination of mercury was covered by the quality assurance programme of research and participation in national and international proficiency tests. Concentrations of total mercury in all investigated samples were found to be generally low, in the range of 0.033-0.577 mg/kg of dry weight and of 0.003-0.045 mg/kg of wet weight. The results indicate that obtained levels of mercury in bivalve molluscs are not likely to pose a risk to the health of consumers.

scholarly journals Effect of genotype and order of farrowing on variation of fertility traits in pigs

2005 ◽  
Vol 21 (3-4) ◽  
pp. 61-68
Author(s):  
Olga Kosovac ◽  
Milica Petrovic ◽  
Branislav Zivkovic ◽  
Mihal Fabjan ◽  
Cedomir Radovic
Keyword(s):  
Statistical Significance ◽  
Variance Analysis ◽  
Test Results ◽  
Large White ◽  
Large White Breed ◽  
Heterosis Effect ◽  
White Breed ◽  
The Difference ◽  
Fertility Traits ◽  
Sire Breed

Investigations were carried out in single pig herd. It included 9 genotypes of sows (4 breeds and 5 groups of crosses of different crossing combinations) and 25.040 of their litters. Traits such as size of litter of Swedish Landrace sows (18.511 litters) and crosses of Fl generation (4651 litters) obtained by crossing of Swedish Landrace (SL) sows and boars of Large White breed (LW) were especially investigated. Second part of the investigation related to effect of sire (SL. LW and D) and dam breed (SL and SLxLW) on average demonstration and variability of number of live born piglets in first seven farrowings. Standard mathematical-statistical methods were applied in order to evaluate average demonstration and variability of the trait litter size. Effect of parent genotype and order of farrowing on variability of traits was evaluated using the variance analysis. Testing of differences in average values was carried out using t-test. Results of the variance analysis (table 2) show that sow genotype and order of farrowing had highly significant effect (P< 0,01) on investigated traits. In investigated herd and between investigated sow-genotypes average number of live born piglets (from 8.50 to 10,37), still born piglets (from 0,30 to 0,79) and reared piglets (from 6,75 to 8,44). Only the difference of average values for number of still born piglets between sows SL and SlxLW had no statistical significance. Investigation of the effect of dam and sire breed on average demonstration and variability of number of live born piglets per farrwoings (table 4) indicates that sows of SL breed mated with boars of LW breed produced more liveborn piglets compared to sows of SL breed mated with SL boars in all farrowings (from +0,30 to +0,69 piglets). These sows, from fourth to seventh farrowing farrowed more than 11 live piglets, which means that individual heterosis effect was displayed (3 to 7%). Mating of sow crosses of Fl generation (SLxLW) with boars of Durroc breed caused no increase of number of live born piglets in litter compared to rearing in pure breed (except in 3rd farrowing) or two breed crossing.

Statistical Significance and Reporting Test Results

1969 ◽  
Vol 6 (4) ◽  
pp. 451-455 ◽  
Author(s):  
Frank M. Rosekrans
Keyword(s):  
Decision Making ◽  
Statistical Significance ◽  
Correct Choice ◽  
Test Results ◽  
The Difference ◽  
Two Alternatives

Presenting the results of experiments as either statistically significant or not significant often ignores useful information for decision making. A more useful figure may be the probability of making the correct choice between two alternatives. Figures that indicate the magnitude of the difference are also important.

scholarly journals The Application of the Online and Offline Interactive Teaching Method in Clinical Anesthesiology Teaching

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Qian Wang ◽  
Congcong Huang ◽  
Xiudan Hu ◽  
Hongxia Mei
Keyword(s):  
Teaching Method ◽  
Statistical Significance ◽  
Control Group ◽  
Test Results ◽  
Interactive Teaching ◽  
The Difference ◽  
Theoretical Test ◽  
Medical University ◽  
Interest Of Learning ◽  
Experimental Group

Objective. To explore whether better teaching effect can be achieved by applying the online and offline interactive teaching method to clinical anesthesiology teaching. Methods. Sixty-three students in grade three majoring in anesthesiology of Wenzhou Medical University were selected as research objects and randomly divided into 2 groups. The experimental group (32 students) adopted the online and offline interactive teaching method. The control group (31 students) adopted the traditional teaching method. The teaching effect was evaluated by the theoretical test results of the two groups and the survey of students in the experimental group. Results. The score of the theoretical test of students in the experimental group (79.31 ± 4.54) was higher than that of students in the control group (75.61 ± 4.88), and the difference achieved statistical significance p < 0.05 . 81.3% of the students think that the interactive teaching method enhances the interest of learning. 90.6% (29/32) of the students in the experimental group hold positive attitude towards the fact that the overall learning effect can be improved by the online and offline interactive teaching method. Conclusions. The online and offline interactive teaching method can not only improve students’ theoretical test results but also help to improve students’ learning participation. Students have given a high score for the evaluation of the online and offline interactive teaching method; good teaching effect has been achieved.

COMPARATIVE EVALUATION OF MULTIPARAMETRIC ENDORECTAL ULTRASOUND AND ENHANCED IMAGING COLONOSCOPY IN THE DIAGNOSIS OF EARLY COLORECTAL CANCER

2020 ◽  
Vol 19 (3) ◽  
pp. 49-64
Author(s):  
E. M. Bogdanova ◽  
Yu. L. Trubacheva ◽  
O. M. Yugai ◽  
S. V. Chernyshov ◽  
E. G. Rybakov ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Statistical Significance ◽  
Muscle Layer ◽  
Endorectal Ultrasound ◽  
Diagnostic Methods ◽  
Early Colorectal Cancer ◽  
Morphological Examination ◽  
Optical Magnification ◽  
The Difference ◽  
Enhanced Imaging

AIM: to compare multiparametric endorectal ultrasound (ERUS) and enhanced imaging colonoscopy in the diagnosis of early colorectal cancer.PATIENTS AND METHODS: the study included 78 patients with epithelial rectal tumor. All the patients underwent multiparametric ERUS and colonoscopy with examination by narrow beam imaging (NBI) at optical magnification. All the patients were operated.RESULTS: a morphological examination removed specimens revealed adenomas in 48 cases, in 19 specimens – adenocarcinomas in situ and T1, and in 11 specimens – adenocarcinomas with invasion of the muscle layer or deeper. When calculating the accuracy indicators of diagnostic methods for groups of patients with adenoma, Tis-T1 adenocarcinoma, and T2-T3 adenocarcinoma, the difference in the sensitivity and specificity of the methods in none of the presented groups did not reach the level of statistical significance (p>0.05).ROC analysis showed that ultrasound has a prognostic value comparable to colonoscopy. The area difference was 0.013 (p=0.85).CONCLUSION: endoscopy and ultrasound have similar value in the diagnosis of malignant transformation of rectal adenomas.

BIOASSAY OF PROLACTIN

1969 ◽  
Vol 60 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Charles S. Nicoll
Keyword(s):  
Epithelial Cells ◽  
Lipid Content ◽  
Dry Weight ◽  
Fat Content ◽  
Assay Sensitivity ◽  
Mucosal Epithelium ◽  
Wet Weight ◽  
Total Dry Weight

ABSTRACT The response of the pigeon crop-sac to systemically acting prolactin (injected subcutaneously) was evaluated by measuring the wet weight of the responsive lateral lobes of the organ and by determining the dry weight of a 4 cm diameter disc of mucosal epithelium taken from one hemicrop. Of several different injection schedules tested, administration of prolactin in four daily injections was found to yield optimal responses. When compared with a graded series of prolactin doses, measurement of the mucosal dry weight proved to be a better method of response quantification than determination of the crop-sac wet weight with respect to both assay sensitivity and precision. The submucosal tissue of the crop-sac was estimated to constitute about 64 % of the total dry weight of the unstimulated organ and it was found to be relatively unresponsive to prolactin stimulation in comparison with the mucosa. The lipid content of the mucosal epithelium was determined using unstimulated crop-sacs or tissues which showed varying degrees of prolactin-induced proliferation. The fat content of the mucosal epithelial cells increased only slightly more rapidly than the dry weight or the defatted dry weight of the mucosa. Suggestions are made for the further improvement of the systemic crop-sac assay for prolactin.

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Cochliobolus Sativus ◽  
Resistance Level ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Barley Genotypes

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

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