GROWTH AND SURVIVAL OF CLARIA CATFISH (CLARIAS GARIEPNUS B., 1822) AT DIFFERENT STAGES OF GROWING WITH ADDITION OF CHICTONIC IN FEED

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
B. Yu. Kovalenko ◽  
V. O. Kovalenko ◽  
D. Yu. Sharylo ◽  
N. V. Polishchuk ◽  
O. A. Korzh ◽  
...  

The paper presents the results of an experiment to assess the effect of different concentrations of vitamin-amino acid complex "Chiktonik" on the growth rate and survival of young African clary catfish (Clarias gariepinus B., 1822) after various stressful situations, for keeping fish in aquaculture. In the experiment, the stressful situation for fish arose due to significant fluctuations in the content of ammonia, nitrites and nitrates in the aquatic environment of a closed recirculation aquasystem, which kept larvae and fry of Clarya catfish during the start of the biological filter, before establishing biological equilibrium in the system. Based on the analysis of sources of scientific and technical information, it was found that the drug "Chiktonik" for more than 10 years is used in animal husbandry to normalize metabolism in animals with unbalanced feeding, after stress, mycotoxin poisoning and antibiotic treatment. This information gave rise to the assumption of the possibility of using the drug in aquaculture, for the same purposes. A series of experiments was performed in a closed recirculating aquasystem, during which the effect of different concentrations of the drug "Chiktonik" in fish feed on larvae and fry of Clarya catfish was tested. It was found that the addition of the drug at the rate of 1 ml per 1 kg of feed accelerates the growth of fish in the experimental variants, compared with the control. Experimental use of high doses of the drug (5, 15, 30 and 45 ml/kg of feed) initially led to inhibition of weight gain of fish, but 10-30 days after the experiment, the growth rate of the experimental material was equal to that of the control group of fish and even exceeded control in the future. The positive effect of vitamin-amino acid complex "Chiktonik" on the survival of young Clarya catfish at the end of the larval period of life and in the early stages of the fry period was established. In an experiment with older fish, which were fully formed fry, such an effect from the use of the drug was not observed: the survival of the fish was at the same level, both in the experiment and in the control. At the same time, it was found that the fry respond less well to high doses of the drug, compared with adult larvae. Thus, the growth rate of fry after the use of high doses of the drug was not equal to that of fish from the control group within a month after the end of the experiment, in contrast to younger fish. In general, the feasibility and safety of the drug "Chiktonik" for young Claria catfish as a feed additive at a dose of 1 ml / kg of fish feed has been proven. It is considered promising to continue research in this direction, using as research material fish of older age groups, including - repair and breeding stock of Claria catfish.

Author(s):  
A. Melnyk ◽  
V. Bezukh ◽  
O. Dubin ◽  
V. Moskalenko ◽  
N. Vovkotrub ◽  
...  

In Ukrainian veterinary medicine the study of the eff ectiveness of new complex drugs remains relevant. The eff ect of the vitamin-mineral complex "Alphabet for Animals" on the state of hemocytopoiesis and the microelements metabolism in calves of 1.5‒2.5 months of age were studied. The composition of this drug includes the biologically active substances: vitamins A, D3, E, B1, B3, B5, B6, B12, K3 and essential amino-acids: DL-methionine, L-lysine and arginine. It is used in farm animals and poultry for the normalization of hemocytopoiesis, the prevention and treatment of vitamins and trace elements metabolism’s disorders. In the fi rst week of drug administration, the number of erythrocytes in the experimental animals group has increased signifi cantly (p<0.05) and after its second feeding, the positive eff ect on erythrocytopoiesis in calves has became even greater (p<0.001), compared to the calves in control group. The level of hemoglobin in calves of experimental group also increased signifi cantly from the beginning of the drug to the end of the experiment, whereas in calves of the control group there was a tendency to decrease its level throughout the all experiment. The color index and erythrocyte saturation by hemoglobin (MCH) at the fi rst and second blood samples were signifi cantly decreased (p<0.05) in control calves group, whereas in animals of experimental group there was no signifi cant diff erence between these parameters (p<0.1). The average volume of red blood cells did not diff er between control and test calves during the experiment (p<0.1). Under the drugs infl uence the Ferum blood content in calves of experimental group increased by 24.2 %. In the control calves group, this tendency was not so noticeable (the increase of Ferum serum level at the end of experiment was only +7.8 % (p<0.1), compared to the initial indices). The Zinc metabolism, on the contrary, has not undergone signifi cant changes. The Cuprum serum level in experimental calves group remained always signifi cantly higher than in control animals group. “Alphabet for Animals” has a positive eff ect on hemocytopoiesis in calves due to its complex composition (fat-soluble and water-soluble vitamins, aminoacids) and improves the metabolism of microelements, including Ferum and Cuprum. The infl uence on these processes the vitamins of B group, which are a part of this drug, we consider especially valuable in the conditions of the unformed rumen digestion in young cattle. Key words: vitamin-amino acid complex, erythrocytopoiesis, hypochromia, microcytosis, hemoglobin, color index, hematocrit.


Author(s):  
N. Vovkotrub ◽  
A. Melnyk ◽  
L. Bogatko ◽  
O. Piddubnyak ◽  
S. Sokolenko

It has been established that the level of blood parameters in the body of sheep is not always stable and often depends on the breed, sex, their physiological state, the level of productivity, feeding and housing conditions. Among the factors that determine the optimal sheep nutritional level, not the last place is occupied by their provision with a sufficient amount of essential amino acids, especially lysine, methionine, cystine, and vitamins, given their physiological importance for the animal body. The overwhelming amount of them is able to be synthesized in the sheep rumen with the help of microorganisms of the gastrointestinal tract, however, some of them are irreplaceable, that is, those whose balance must be replenished through feed rations. The article describes the use of the vitamin-amino acid complex "Аlphabet for Animals" in sheep, containing a complex of fat-and water-soluble vitamins, as well as amino acids, which contribute to the normalization of metabolism in the body of small ruminants, increase its resistance, have a positive effect on productivity, safety and reproductive animals functions. During the research, the effect of this feed additive on hemocytopoiesis and the exchange of essential micronutrients in ewes were studied. After a double use of the additive, an increase in the processes of erythrocytopoiesis was established, as evidenced by an increase in the number of blood erythrocytes by 21.2% and the hemoglobin content by 11.2%, stabilization of the size and degree of saturation of erythrocytes with hemoglobin, as evidenced by a decrease in MCH and MCV indices. There were no significant changes in the content of essential micronutrients in the blood serum of sheep under the influence of the "Alphabet for Animals", and a slight increase of zinc content in the blood was noted in sheep after the second injection of the drug. At the same time, in the blood of experimental animals group a significant increase the iron content on average up to 129.5±7.33 μg/100 ml (p<0.05) was established, and the number of animals with a low amount of blood iron was only 12.5 %. The number of leukocytes in the sheep blood of both groups was within the normal range and during the second and third blood tests did not change significantly. Key words: sheep, feeding, feed additives, amino acids, vitamins, hemocytopoiesis, essential micronutrients.


2003 ◽  
Vol 23 (1) ◽  
pp. 25-32
Author(s):  
K. M. Downs ◽  
R. A. Norton ◽  
K. S. Macklin ◽  
J. B. Hess

Development ◽  
1965 ◽  
Vol 13 (3) ◽  
pp. 341-356
Author(s):  
F. S. Billett ◽  
Rosalba Collini ◽  
Louie Hamilton

In many bacterial systems chloramphenicol has been shown to inhibit protein synthesis (Hahn & Wisseman, 1951; Gale & Folkes, 1953). The precise mechanism of this inhibition is not clear, although the evidence suggests that the interaction of the soluble RNA-amino acid complex with the ribosomes is prevented because the attachment of the messenger RNA to the ribosomes is itself impaired (Lacks & Gros, 1959; Nathans & Lipman, 1961; Jardetsky & Julian, 1964; Julian & Jardetsky, 1964). In contrast to its effect on bacterial systems, chloramphenicol has been reported to have little or no action on the protein synthesis by cell-free extracts of mammalian cells (Rendi, 1959; Ehrenstein & Lipmann, 1961). A basis for this resistance has been proposed by Vazquez (1964), who finds that whereas bacterial ribosomes bind chloramphenicol, ribosomes from other organisms do not. Nevertheless, it cannot be stated with any confidence that chloramphenicol has no effect on the protein synthesis of animal cells.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 82-83
Author(s):  
Pornpim Aparachita ◽  
Scott Carter ◽  
Afton Sawyer ◽  
Jared Harshman ◽  
Zach Rambo ◽  
...  

Abstract Previously, we reported that supplementing a water soluble zinc via drinking water (0 to 80 mg/L) to nursery pigs improved ADG and G:F. To evaluate the efficacy of higher titrated levels of this water soluble zinc amino acid complex (ProPath®Zn LQ, Zinpro Corporation, Eden Prairie, MN) on growth performance, 280 crossbred pigs (5.5 kg BW; 19 d of age) were randomly allotted to four water treatments (7 pens/treatment; 10 pigs/pen). The water treatments were 0, 40, 80 and 160 mg Zn/L of water. Pigs were fed in 4 dietary phases with complex, nutrient-dense, corn-soybean meal-based diets: Phase 1 and 2 (2,500 and 1,750 mg Zn as ZnO/kg; d 1–7 and 7–14, respectively) and Phase 3 and 4 (200 mg Cu as CuSO4/kg; d 14–23 and 23–42, respectively). Pigs and feeders were weighed weekly to determine ADG, ADFI, and G:F. Water meters were used to record and calculate water disappearance and zinc intake. Data were analyzed as a randomized complete block design. Orthogonal polynomial contrasts were used to determine linear and curvilinear effects. Water and total zinc intake increased linearly (P < 0.001) with increasing water zinc concentration. From d 0–14 when high dietary zinc was fed, there were no differences (P > 0.10) in ADG, ADFI, or G:F. However, from d 14–42 when basal levels of zinc were fed, quadratic improvements in ADG (0.545, 0.561, 0.578, 0.546 kg; P < 0.05) and G:F (0.686, 0.706, 0.723, 0.702; P < 0.01) were observed with increasing zinc via water. Similarly for d 0–42, ADG (0.435, 0.440, 0.454; 0.434 kg; P = 0.07), G:F (0.726, 0.740, 0.763, 0.749; P = 0.05) and average ending wt (23.73, 23.97, 24.55, 23.70 kg; P = 0.07) improved quadratically with increasing zinc. In conclusion, supplementing ProPath®Zn LQ via water resulted in improvements in ADG and G:F for nursery pigs.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 212-213
Author(s):  
Chanda Engel ◽  
Gary Tibbetts ◽  
Mark Branine

Abstract A 42-d research study evaluated effects of feeding beef steers increasing levels of dietary Zn from Zn methionine (AAC-Zn; ZINPRO, Zinpro Corporation, Eden Prairie, MN) with or without supplemental Cu from Cu amino acid complex (AAC-Cu; Availa Cu, Zinpro Corp.) concurrently with ractopamine hydrochloride (RAC; Optaflexx, Elanco Animal Health, Greenfield, IN). One hundred-twenty steers (mean BW = 624 kg), ≤ 50 d from projected harvest were randomized to three pens (40 steers/pen) equipped with GrowSafe Systems (Calgary AB, Canada) feed bunk technology. Each pen was assigned to one of three treatment diets: 1) basal finishing diet + 30 mg Zn/kg DM from AAC-Zn (CON); 2) basal finishing diet + 90 mg Zn/kg DM from AAC-Zn (AAC-Zn90); or 3) AAC-Zn90 diet + 10 mg Cu/kg DM from AAC-Cu (AAC-Zn/Cu). Following allocation to treatments, cattle were acclimated to pen cohorts and GrowSafe feed bunks for 7 days. All steers were fed 300 mg RAC∙hd-1∙d-1 starting 35 d prior to harvest. Individual feed intake measurements began with RAC feeding and continued for 35 d until cattle were shipped for harvest. Carcass data were collected from each steer. Data were analyzed with individual animal as the experimental unit using PROC MIXED and PROC GLIMMIX procedures of SAS 9.4 (SAS Institute, Cary, NC). Numerically AAC-Zn90 fed cattle had heavier carcass weights than CON and AAC-Zn/Cu. Steers fed AAC-Zn90 had greater (P = 0.02) marbling scores compared to AAC-Zn/Cu. Steers fed AAC-Zn90 had lower backfat thickness (P = 0.02) and numerically greater marbling scores compared to CON. A biphasic program of feeding a lower level of AAC-Zn for the duration of the finishing phase followed by an increased rate of AAC-Zn during RAC feeding may optimize overall live and carcass response and improve total individual animal value.


2003 ◽  
Vol 5 (2) ◽  
pp. 11
Author(s):  
Rustidja Rustidja

The aim of study was to know the effect of sludge in the ration on the gonadal growth of Oreochromis sp. broodstock. Experimental design used in this experiment was completely Randomized Design. The broodstocks were fed with the ration containing 0-40% of sludge, or with a commercial fish feed as a control.The results were not significantly differences between the treatment in all parameters. The sludge in ration ranging from 0 to 40% resulted the level of gonadal maturation of 4, 6, 7, dan 9. The value of Gonadal Maturation Index on 0,96 to 3,98 % and the control group on 1,75 %. The specific growth rate of broodstock fed with the ration ranged from 1,1 to 1,62 Bw/day and that of the development of gonadal control group was 1,97. The survival rate were variated between 14,29 to 100% and that of the development of gonadal control group is 71,43%. The ration containing 40% sludge is the most suitable feed for maturation in Oreochromis.


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