Synthesis of Paclitaxel Loaded on Nanoparticles of Archaeosomes and Investigation of Its Anti-cancer Properties

Objective: In this study, the effect of archaeosomal paclitaxel drug on breast cancer MDA-MB231 cell lines was evaluated. Material and method: The experiments were conducted to assess the cytotoxicity of paclitaxel in free form and compare it with archaeosomal form of the drug in vitro. To check the size of archaeosomes, Zetasizer device (DLS) was used. The average size of archaeosom nanoparticles containing paclitaxel drugs 430 nm and surface charge -15 and the average size of control archaeosom nanoparticles without drug 460 nm and surface charge -14 were reported. The effect of archaeosomal drug containing paclitaxel and free drug were examined by MTT test.Results: In both formulations, zeta potential was negative. Nano-archaeosom containing paclitaxel drug had higher physical stability compared with control nano-Archaeosom. Tests showed the killing effect of nano-archaeosome containing paclitaxel formulation tumor cells was more than free formulation. In the other word, nano-archaeosome containing paclitaxel is more effective than free paclitaxel.Conclusion: The experiments showed that the killing effect of tumor cells of nano-archaeosomes formulations containing paclitaxel was more than free formulation. That is, nano-archaeosomes containing paclitaxel was more effective than free form paclitaxel.

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Thymoquinone and its nanoformulation attenuate colorectal and breast cancers and alleviate doxorubicin-induced cardiotoxicity

Nanomedicine ◽

10.2217/nnm-2021-0103 ◽

2021 ◽

Author(s):

Ali H El-Far ◽

Taher A Salaheldin ◽

Kavitha Godugu ◽

Noureldien HE Darwish ◽

Shaker A Mousa

Keyword(s):

Xenograft Model ◽

Double Emulsion ◽

Tumor Model ◽

Breast Cancers ◽

Anti Cancer ◽

Xenograft Tumor Model ◽

Average Size ◽

Hct116 Cells

Aim: To investigate the anti-cancer potential of thymoquinone (TQ) and TQ nanoparticles (TQ-NPs) and their protection against doxorubicin (DOX)-induced cardiotoxicity. Methods: TQ-NPs were prepared by double emulsion method and characterized. The efficacy of TQ and TQ-DOX was studied against HCT116 and MDA-MB-231-Luc cancer cell lines in vitro and in a xenograft tumor model. Results: TQ and TQ + DOX increased Bax levels in HCT116 cells and decreased Bcl2 levels in MDA-MB-231-Luc cells. In the xenograft model, the TQ-NPs, with an average size of 218 nm, in combination with DOX, significantly reduced tumor size. The combination of TQ or TQ-NPs with DOX significantly reduced DOX-induced cardiotoxicity. Conclusion: Data suggest the promising role of TQ and TQ-NPs alone and with DOX for anti-cancer and cardiac protection benefits.

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Preparation of liposomal nanocarrier by extruder to enhance tumor accumulation of paclitaxel

Journal of Bioactive and Compatible Polymers ◽

10.1177/08839115211053926 ◽

2021 ◽

pp. 088391152110539

Author(s):

Ngoc Thuy Trang Le ◽

Ngoc Hoi Nguyen ◽

Minh Chau Hoang ◽

Cuu Khoa Nguyen ◽

Dai Hai Nguyen ◽

...

Keyword(s):

Wide Spectrum ◽

Dependent Manner ◽

Liposome Suspension ◽

High Uniformity ◽

Anti Cancer ◽

Effective Delivery ◽

Tumor Accumulation ◽

Average Size ◽

Low Solubility

Despite the wide-spectrum and effective anti-cancer activity of paclitaxel (PTX), their low solubility and side effects are the main challenges in their clinical application. In this study, a model paclitaxel-encapsulated nanoliposome (NLips-PTX) carrier was synthesized to enhance PTX solubility and increase its passive accumulation at the tumor site. Soy lecithin and cholesterol at a 9:1 ratio were used to prepare the nano-sized liposomes through the thin-film hydration followed by extrusion technique. The prepared spherical NLips-PTX liposomes with an average size of about 150 nm and high uniformity were characterized by DLS and TEM. PTX load efficiency of NLips was determined at about 85% by HPLC. NLips-PTX also showed a therapeutic effect toward breast cancer cells (MCF-7) in a dose- and time-dependent manner via in vitro cellular uptake and a cytotoxicity study. This research indicates that extrusion is a simple and convenient method for nano-sizing and homogenising liposome suspension for potentially effective delivery of drug to target tumor sites.

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Preparation of wormlike polymeric nanoparticles coated with silica for delivery of methotrexate and evaluation of anticancer activity against MCF7 cells

Journal of Biomaterials Applications ◽

10.1177/0885328217698063 ◽

2017 ◽

Vol 31 (9) ◽

pp. 1305-1316 ◽

Cited By ~ 31

Author(s):

Farhad Gharebaghi ◽

Naser Dalali ◽

Ebrahim Ahmadi ◽

Hossein Danafar

Keyword(s):

Polymeric Nanoparticles ◽

Drug Loading ◽

Free Drug ◽

Spherical Nanoparticles ◽

Mcf7 Cells ◽

Anti Cancer ◽

The Impact ◽

Cancer Activity ◽

Mcf 7

Methotrexate is one of the most effective drugs that is commonly used in the treatment of cancer. However, its application is limited due to low solubility, high toxicity and rapid metabolism. Therefore, in the present study, worm-like polymeric nanoparticles as carrier of methotrexate were prepared using biodegradable copolymers (mPEG–PCL). The impact of nanoparticles’ geometry on the loading, delivery and drug’s anti-cancer activity was investigated. The di-block copolymer mPEG–PCL was being synthesized by a ring opening polymerization of ɛ-caprolactone in the presence of mPEG as the initiator and Sn(oct)2 as the catalyst. It was used for the preparation of worm-like micelles and coated with silica, so that their structures are stable after drying. The synthesized copolymers and nanoparticles were characterized by FTIR, HNMR, GPC, XRD, TGA, DLS, and FE-SEM analyses. The efficiencies of drug loading and release of nanoparticles as in vitro, was studied by high performance liquid chromatography. The MTT method was used to estimate the toxicity on MCF-7 cell category. The obtained results showed that the nanoparticles were worm-like particles with less than 150 nm diameter and about 1 µm length. The loading and encapsulation efficiencies of drug by the worm-like nanoparticles were 3.5 ± 0.14% and 65.6 ± 0.12%, respectively, while they were obtained as 2.1 ± 0.08% and 26 ± 0.10%, respectively, for spherical nanoparticles. The methotrexate diffusional behavior of worm-like nanoparticles was compared with that of the spherical ones. On the other hand, the anti-cancer activity of MTX-loaded nanoparticles was more than the free drug. The results of the MTT assay showed strong and dose-dependent inhibition of cell (MCF-7 category) growth by the nanoparticles compared with MTX. The inhibitory concentrations (IC50 i.e. reduction viability of cell to 50%) obtained for worm-like, spherical nanoparticles and free drug (incubation times 72 h) were 8.25 ± 0.20, 9.15 ± 0.17, 12.28 ± 0.15 µg/mL, respectively. It can be concluded that application of non-spherical nanoparticles is a better and more effective strategy for controlled and slow release of methotrexate in the treatment of cancer.

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Carrier-Free Multifunctional Nanomedicine For Intraperitoneal Disseminated Ovarian Cancer Therapy

10.21203/rs.3.rs-1192880/v1 ◽

2022 ◽

Author(s):

Xiuyu Huang ◽

Miaojuan Qiu ◽

Tianqi Wang ◽

Binbin Li ◽

Shiqiang Zhang ◽

...

Keyword(s):

Ovarian Cancer ◽

Tumor Cell ◽

Tumor Cells ◽

Self Assembly ◽

Gynecological Cancer ◽

Advanced Ovarian Cancer ◽

Malignant Ascites ◽

Tumor Cell Migration ◽

Average Size

Abstract Background: Ovarian cancer is the most lethal gynecological cancer which is characterized by extensive peritoneal implantation metastasis and malignant ascites. Despite advances in diagnosis and treatment in recent years, the five-year survival rate is only 25 - 30%. Therefore, developing multifunctional nanomedicine with abilities of promoting apoptosis and inhibiting migration on tumor cells would be a promising strategy to improve the antitumor effect.Methods and results: In this study, we developed a novel ACaT nanomedicine composed of alendronate, calcium ions and cyclin-dependent kinase 7 (CDK7) inhibitor THZ1. With the average size of 164 nm and zeta potential of 12.4 mV, the spherical ACaT nanoparticles were selectively internalized by tumor cells and effectively accumulated in the tumor site. Results of RNA-sequencing and in vitro experiments showed that ACaT promoted tumor cell apoptosis and inhibited tumor cell migration by arresting the cell cycle, increasing ROS and affecting calcium homeostasis. Weekly intraperitoneally administered of ACaT for 8 cycles significantly inhibited the growth of tumor and prolonged the survival of intraperitoneal xenograft mice.Conclusion: In summary, this study presents a new self-assembly nanomedicine with favorable tumor targeting, antitumor activity and good biocompatibility, providing a novel therapeutic strategy for advanced ovarian cancer.

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Anti-cancer effect of a novel 2,3-didithiocarbamate-substituted naphthoquinone as a tumor metabolic suppressor in vitro and in vivo

MedChemComm ◽

10.1039/c8md00062j ◽

2018 ◽

Vol 9 (4) ◽

pp. 632-638 ◽

Cited By ~ 1

Author(s):

Xianling Ning ◽

Yunqiao Li ◽

Hailong Qi ◽

Ridong Li ◽

Yan Jin ◽

...

Keyword(s):

Tumor Cell ◽

Tumor Cells ◽

Cell Metabolism ◽

Anti Cancer ◽

Tumor Cell Metabolism

Suppressing tumor cell metabolism is an attractive strategy for treating cancer. We identified a 2,3-didithiocarbamate-substituted naphthoquinone 3i that inhibited the proliferation of tumor cells by disturbing their metabolism.

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Adhesion-based simple capture and recovery of circulating tumor cells using a blood-compatible and thermo-responsive polymer-coated substrate

RSC Advances ◽

10.1039/c6ra15229e ◽

2016 ◽

Vol 6 (92) ◽

pp. 89103-89112 ◽

Cited By ~ 11

Author(s):

Takashi Hoshiba ◽

Toshihiko Orui ◽

Chiho Endo ◽

Kazuhiro Sato ◽

Ayano Yoshihiro ◽

...

Keyword(s):

Tumor Cells ◽

Circulating Tumor Cells ◽

Cancer Biology ◽

Metastatic Cancer ◽

Cancer Diagnostics ◽

Cancer Drug ◽

Coated Substrate ◽

Anti Cancer ◽

Biology Research

Circulating tumor cells (CTCs) have been a focus of study for metastatic cancer diagnostics, in in vitro anti-cancer drug screening to decide the chemotherapeutic course, and cancer biology research.

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Expression of the apelinergic system and its influence on functional properties of tumor cells

Genetics & Applications ◽

10.31383/ga.vol5iss1pp26-39 ◽

2021 ◽

Vol 5 (1) ◽

pp. 26

Author(s):

Zoran Knezevic ◽

Jasmin Wellbrock ◽

Frauke Fuchs ◽

Gabi Vohwinkel ◽

Antonia Matzat ◽

...

Keyword(s):

Tumor Cells ◽

Cell Lines ◽

Functional Properties ◽

Cancer Survival ◽

Promising Target ◽

Anti Cancer ◽

Wildtype Cell ◽

Apelin Receptor

Small peptide Apelin and its cognate receptor APJ, are known to play a role in tumor angiogenesis and overall cancer progession. Certain authors suggest that the Apelin receptor is also a factor in cancer immunotherapy. In this article, our goal was to study the effects of in vitro targeting of the Apelin/APJ system on the tumor cells functional properties. Protein surface and mRNA expression of Apelin and APJ had been largely examined in various tumor-derived cell lines. In contrast to the tumor tissue, the results of this study demonstrated that most tumor cell lines exhibited somewhat moderate expression of Apelin/APJ. Similar effects of APJ stimulation and inhibition had been observed in in vitro functional assays, which was due to their unusually low expression levels. Low APJ expression in cell lines has been overcome by stable APJ overexpression. In such conditions, stimulation of apelinergic system APJ-overexpressed cells affected cell functional properties in comparison to the wildtype cell lines, where overexpression of APJ receptor resulted in increased migration. On the other hand, no effect on cell proliferation was observed. Consequently, Apelin/APJ signaling in tumor-derived cell lines is not expected to play a direct and crucial role in in vitro cancer survival. Further investigation should focus on in vivo role of the apelinergic system, as demonstrated in the recently published studies, where apelinergic system is claimed to be a promising target for anti-cancer therapy.

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Garcinol Exhibits Anti-Neoplastic Effects by Targeting Diverse Oncogenic Factors in Tumor Cells

Biomedicines ◽

10.3390/biomedicines8050103 ◽

2020 ◽

Vol 8 (5) ◽

pp. 103 ◽

Cited By ~ 5

Author(s):

Vaishali Aggarwal ◽

Hardeep Singh Tuli ◽

Jagjit Kaur ◽

Diwakar Aggarwal ◽

Gaurav Parashar ◽

...

Keyword(s):

Tumor Cells ◽

Clinical Stage ◽

Therapeutic Index ◽

In Vivo Studies ◽

Regulation Of Transcription ◽

Cellular Processes ◽

Garcinia Indica ◽

Anti Cancer

Garcinol, a polyisoprenylated benzophenone, is the medicinal component obtained from fruits and leaves of Garcinia indica (G. indica) and has traditionally been extensively used for its antioxidant and anti-inflammatory properties. In addition, it has been also been experimentally illustrated to elicit anti-cancer properties. Several in vitro and in vivo studies have illustrated the potential therapeutic efficiency of garcinol in management of different malignancies. It mainly acts as an inhibitor of cellular processes via regulation of transcription factors NF-κB and JAK/STAT3 in tumor cells and have been demonstrated to effectively inhibit growth of malignant cell population. Numerous studies have highlighted the anti-neoplastic potential of garcinol in different oncological transformations including colon cancer, breast cancer, prostate cancer, head and neck cancer, hepatocellular carcinoma, etc. However, use of garcinol is still in its pre-clinical stage and this is mainly attributed to the limitations of conclusive evaluation of pharmacological parameters. This necessitates evaluation of garcinol pharmacokinetics to precisely identify an appropriate dose and route of administration, tolerability, and potency under physiological conditions along with characterization of a therapeutic index. Hence, the research is presently ongoing in the dimension of exploring the precise metabolic mechanism of garcinol. Despite various lacunae, garcinol has presented with promising anti-cancer effects. Hence, this review is motivated by the constantly emerging and promising positive anti-cancerous effects of garcinol. This review is the first effort to summarize the mechanism of action of garcinol in modulation of anti-cancer effect via regulation of different cellular processes.

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Role of Short- and Long-Lived Reactive Species on the Selectivity and Anti-Cancer Action of Plasma Treatment In Vitro

Cancers ◽

10.3390/cancers13040615 ◽

2021 ◽

Vol 13 (4) ◽

pp. 615

Author(s):

Kyriakos Sklias ◽

João Santos Sousa ◽

Pierre-Marie Girard

Keyword(s):

Lipid Peroxidation ◽

Cell Death ◽

Phase I ◽

Plasma Treatment ◽

Tumor Cells ◽

Phase Ii ◽

Treatment Modalities ◽

Normal Cells ◽

Anti Cancer

(1) Plasma-activated liquids (PAL) have been extensively studied for their anti-cancer properties. Two treatment modalities can be applied to the cells, direct and indirect plasma treatments, which differ by the environment to which the cells are exposed. For direct plasma treatment, the cells covered by a liquid are present during the plasma treatment time (phase I, plasma ON) and the incubation time (phase II, plasma OFF), while for indirect plasma treatment, phase I is cell-free and cells are only exposed to PAL during phase II. The scope of this work was to study these two treatment modalities to bring new insights into the potential use of PAL for cancer treatment. (2) We used two models of head and neck cancer cells, CAL27 and FaDu, and three models of normal cells (1Br3, NHK, and RPE-hTERT). PBS was used as the liquid of interest, and the concentration of plasma-induced H2O2, NO2− and NO3−, as well as pH change, were measured. Cells were exposed to direct plasma treatment, indirect plasma treatment or reconstituted buffer (PBS adjusted with plasma-induced concentrations of H2O2, NO2−, NO3− and pH). Metabolic cell activity, cell viability, lipid peroxidation, intracellular ROS production and caspase 3/7 induction were quantified. (3) If we showed that direct plasma treatment is slightly more efficient than indirect plasma treatment and reconstituted buffer at inducing lipid peroxidation, intracellular increase of ROS and cancer cell death in tumor cells, our data also revealed that reconstituted buffer is equivalent to indirect plasma treatment. In contrast, normal cells are quite insensitive to these two last treatment modalities. However, they are extremely sensitive to direct plasma treatment. Indeed, we found that phase I and phase II act in synergy to trigger cell death in normal cells and are additive concerning tumor cell death. Our data also highlight the presence in plasma-treated PBS of yet unidentified short-lived reactive species that contribute to cell death. (4) In this study, we provide strong evidence that, in vitro, the concentration of RONS (H2O2, NO2− and NO3−) in combination with the acidic pH are the main drivers of plasma-induced PBS toxicity in tumor cells but not in normal cells, which makes ad hoc reconstituted solutions powerful anti-tumor treatments. In marked contrast, direct plasma treatment is deleterious for normal cells in vitro and should be avoided. Based on our results, we discuss the limitations to the use of PAL for cancer treatments.

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Fucoidan-Based Nanoparticles with Inherently Therapeutic Efficacy for Cancer Treatment

Pharmaceutics ◽

10.3390/pharmaceutics13121986 ◽

2021 ◽

Vol 13 (12) ◽

pp. 1986

Author(s):

Chih-Sheng Chiang ◽

Bo-Jie Huang ◽

Jui-Yu Chen ◽

Wee Wei Chieng ◽

Seh Hong Lim ◽

...

Keyword(s):

Breast Cancer ◽

Therapeutic Efficacy ◽

Safety Information ◽

Therapeutic Window ◽

Free Form ◽

Control Group ◽

Anticancer Properties ◽

Rapid Clearance ◽

Average Size

The anticancer properties of fucoidan have been widely studied in cancer research. However, the lack of safety information on the parenteral administration of fucoidan and its rapid clearance from the system have limited its application. Herein, we assessed the therapeutic efficacy and safety of fucoidan and developed fucoidan nanoparticles (FuNPs) to enhance their therapeutic effect in the mouse model of breast cancer. FuNPs were synthesized through the emulsion method, and the stable colloid has an average size of 216.3 nm. FuNPs were efficiently internalized into breast cancer cells in vitro, demonstrating an enhanced antitumor activity in comparison with free form fucoidan. After the treatment of FuNPs, the tumor progression was significantly inhibited in viv. The tumor volume was reduced by 2.49-fold compared with the control group. Moreover, the inhibition of the invasion of tumor cells into the lungs revealed the antimetastatic properties of the FuNPs. FuNPs, as naturally marine polysaccharide-based nanoparticles, have shown their broader therapeutic window and enhanced antimetastatic ability, opening an avenue to the development of the inherently therapeutic nanomedicines.

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