The Main Clinical, Laboratory and Immunological Parameters in Patients with Leptospirosis in Saint-Petersburg

2018 ◽  
pp. 725-727
Author(s):  
O. Petrova ◽  
N. Stoyanova ◽  
V. Basina ◽  
D. Dzyuban ◽  
N. Lubimova ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Immunological Parameters ◽  
Saint Petersburg

scholarly journals Results of monitoring analisis of immunological parameters in employees of production sites located at the former mining and uranium ore processing territories

2018 ◽  
Vol 15 (6) ◽  
pp. 54-64
Author(s):  
L V Luss ◽  
S V Tsarev ◽  
V I Astafurov ◽  
O I Sidorovich ◽  
M F Nikonova ◽  
...  
Keyword(s):  
Viral Infections ◽  
Clinical Laboratory ◽  
Statistical Processing ◽  
Immunological Methods ◽  
Immunological Parameters ◽  
Uranium Ore ◽  
Ore Processing ◽  
Significant Difference ◽  
Immunological Examination ◽  
Mechanical Plant

Aim. To study cellular and humoral immunological parameters of the employees of production sites located at the former mining and uranium ore processing territories. Materials and methods. In this work the clinical laboratory and immunological methods of examination were used. Statistical processing of the results was performed by using nonparametric analysis methods in the StatSoft Statistica 6 software package. Results. 101 employees of Chepetskiy mechanical plant were examined at the age of 19-62. 76 (75.2%) of them contacted with industrial factor, 64 - with radiation and 12 - with chemical. 25 (24.8%) employees had no contact with industrial factor. The immunological examination of the employees in contact with industrial factor revealed laboratory signs of imbalance in the immune system with IgE-overproduction. The risk group for secondary immunodeficiency with the leading infectious syndrome was 15.8%. Conclusion. Changes of cellular and humoral parameters on average values were not revealed. Infectious syndrome - recurrent ETN viral infections (13.9%) and herpes virus infection (13.9%) prevailed in the structure of the employees of Chepetskiy mechanical plant. A statistically significant difference in the content (percentage and absolute) of CD16+ lymphocytes was found in the employees of Chepetskiy mechanical plant contacted with industrial factor. These results had no clinical significance, what can be considered as an adaptive response to the chemical factor.

scholarly journals SAS Enterprise Guide 6.1 for physicians: correlation analysis

2020 ◽  
Vol 20 (1) ◽  
pp. 51-56
Author(s):  
Nikolay S. Bunenkov ◽  
Gulnara F. Bunenkova ◽  
Vladimir V. Komok ◽  
Oleg A. Grinenko ◽  
Alexander S. Nemkov
Keyword(s):  
Clinical Trial ◽  
Data Analysis ◽  
Correlation Analysis ◽  
Randomized Clinical Trial ◽  
Clinical Laboratory ◽  
Analysis Data ◽  
Saint Petersburg ◽  
Develop Algorithm ◽  
Efficient Data ◽  
Medical University

Objective: to develop algorithm of correlation analysis of prospective non-randomized clinical trial AMIRICABG (ClinicalTrials.gov Identifier: NCT03050489) data using SAS Enterprise Guide 6.1. Materials and methods. Data collection was performed according prospective non-randomized clinical trial AMIRICABG (ClinicalTrials.gov Identifier: NCT03050489) in Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia between 20162019 years with 336 patients. There is database with clinical, laboratory and instrumental data. Correlation analysis was performed with SAS Enterprise Guide 6.1. Results. There was developed algorithm of correlation analysis data of prospective non-randomized clinical trial AMIRICABG (ClinicalTrials.gov Identifier: NCT03050489). This algorithm could be useful for physicians and researchers for data analysis. Conclusion. Presented algorithm of correlation analysis could make easier and improve efficient data analysis with SAS Enterprise Guide 6.1.

Evaluating of prognostic value of biomarker and upper reference limit calculating

2020 ◽  
Vol 20 (2) ◽  
pp. 79-86
Author(s):  
Nikolay S. Bunenkov ◽  
Gulnara F. Bunenkova ◽  
Vladimir V. Komok ◽  
Oleg A. Grinenko ◽  
Alexander S. Nemkov
Keyword(s):  
Logistic Regression ◽  
Data Analysis ◽  
Prognostic Value ◽  
Troponin I ◽  
Clinical Laboratory ◽  
Artery Bypass ◽  
Youden Index ◽  
Reference Limit ◽  
Saint Petersburg ◽  
Upper Reference Limit

Objective: to develop algorithm of assessment of prognostic value of biomarker (troponin I) for predicting death after coronary artery bypass grafting. Materials and methods. Data collection was performed according to prospective non-randomized clinical trial AMIRI CABG in Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia between 20162019 years with 336 patients. There is database with clinical, laboratory and instrumental data. Statistics were calculated with SAS Enterprise Guide 6.1 software. Prognostic capability of biomarker for death were evaluated with logistic regression. Spline of relation between death and biomarker level were plotted using coefficient of logistic regression and intercept. Upper reference limit was calculated with Youden index. Results. There was developed algorithm to assess prognostic value of biomarker and its usefulness for clinical application and to define upper reference limit of biomarker. This algorithm could be useful for physicians and researchers for data analysis. Conclusion. Presented algorithm of data analysis allows to assess prognostic value of novel biomarker and its clinical usefulness.

scholarly journals High Levels of anti-Leishmania IgG3 and Low CD4+ T Cells Count were Associated with Relapses in Visceral Leishmaniasis

2020 ◽  
Author(s):  
Renata Kuschnir ◽  
Leonardo Pereira ◽  
Maria Rita Dutra ◽  
Ludmila de Paula ◽  
Maria Luciana Silva-Freitas ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
B Lymphocyte ◽  
Clinical Laboratory ◽  
Clinical Symptoms ◽  
Laboratory Data ◽  
Active Phase ◽  
Post Treatment ◽  
Clinical Markers ◽  
Immunological Parameters ◽  
Cell Counts

Abstract Background: Visceral leishmaniasis (VL) is severe and potentially fatal. Brazil is one of the countries with the greatest endemicity for the disease in the world. The reduction of CD4+ T lymphocytes, B cells activation and high levels of inflammatory cytokines (IL-6/IL-8/TNF/IL-1β), plasma LPS, soluble CD14, anti-Leishmania IgG3 and low leptin levels are involved in the immunopathogenesis of VL, most associated with severe VL. Despite relapses occurring in about 4-5% of patients with VL not associated with HIV infection, the factors underlying relapses are little known. Our aim was to identify clinical, laboratory and immunological parameters that may be associated with recurrences in VL.Methods: Fifteen VL patients recruited from Hospital Eduardo de Menezes (BH-MG) were grouped into relapsing (R-VL, n=5) and non-relapsing (NR-VL, n=10) and evaluated during active disease, immediately after treatment (post-treatment) and six months post-treatment (6mpt). Clinical and laboratory data obtained from medical records were correlated with CD4+ and CD8+ T cell counts and anti-Leishmania Igs and IL-6 plasma levels and compared to those parameters of ten healthy controls.Results: During the active phase of VL, despite similarity in the clinical symptoms, the rates of thrombocytopenia, elevated transaminases (AST and ALT) and hyperbilirubinemia were higher in the NR-VL group compared to R-VL (p<0.05), a profile reversed during the post-treatment phase. All patients had low CD4+ T counts in active phase, however, NR-VL patients had a higher gain of this cell type than R-VL in the post-treatment (p<0.05). There was a significant reduction in IgG3 levels during the follow-up in the NR-VL group compared to the R-VL, especially at 6mpt (p<0.05). In addition, IgG3 levels were negatively correlated with CD4+ T counts in the R-VL group (r=-0.52). Elevated levels of IL-6 were observed in active VL and correlated with clinical markers of severity. Conclusions: During active phase of VL, the NR-VL patients presented more severe laboratorial abnormalities compared to R-VL, probably because the latter had already received previous treatment. On the other hand, R-VL exhibited greater impairment of immune reconstitution and a high degree of B lymphocyte activation, which must be a factor that favored relapses.

scholarly journals Differentiated Approach to the Diagnosis and Treatment of Tuberculous Spondylitis in Adults

2021 ◽  
Author(s):  
Primkul Nazirov ◽  
Alfiya Fakhridinova ◽  
Zulfiya Makhmudova ◽  
Bakhtiyar Djuraev
Keyword(s):  
Immune System ◽  
Clinical Laboratory ◽  
Medical Center ◽  
Spinal Tuberculosis ◽  
Spinal Column ◽  
Response To Treatment ◽  
Immunological Study ◽  
Immunological Parameters ◽  
Spinal Disorders ◽  
Functional Changes

The purpose of this study is to generalize the results of a complex clinical-laboratory, radiological and immunological study of patients with spinal tuberculosis. A comprehensive examination analysis was carried out in 192 patients with tuberculous spondy litis who were in the department of surgery for osteoarticular tuberculosis of the Republican Specialized Scientific and Practical Medical Center of Tuberculosis and Pulmonology in Tashkent, Uzbekistan. Severe degrees of spinal disorders were found in 48.7% of patients. Magnetic resonance imaging (MRI) and multi-slice spiral computed tomography (MSCT) allow establishing the stage and activity of a specific process in the spine, as well as complications. Spinal column instability was detected in 143 (74.5%) patients, paravertebral abscesses in 116 (60.4%) patients, and epidural abscesses in 75 (39.0%) patients. Changes in immunological parameters in patients with spinal tuberculosis were associated both with impaired regulation of immunogenetic and with the direct influence of the immune system at various stages of the process. The detection of certain patterns of immune system disorders in patients with tuberculous lesions of the spine allowed us to make a differentiated approach to their treatment in order to increase the effectiveness of the treatment. It can be concluded that at present, tuberculosis of bones and joints, especially the spine, is detected in an advanced stage in 50-80% of cases and has a widespread and complicated character with profound anatomical and functional changes and the presence of severe spinal disorders in 48.7% patients in Uzbekistan. Regarding spondylitis, it is immensely important to make an accurate diagnosis based on the clinical presentations, laboratory, radiological and immunological findings and by periodic assessment of the response to treatment, which is essential in atypical cases.

scholarly journals Clinical and diagnostic features of dengue haemorrhagic fever in children

2018 ◽  
Vol 5 (3) ◽  
pp. 791
Author(s):  
Gomathi Chenna Reddy ◽  
Nagendra K.
Keyword(s):  
Platelet Count ◽  
Serum Albumin ◽  
Liver Enzymes ◽  
Clinical Laboratory ◽  
Diagnostic Tools ◽  
Severe Bleeding ◽  
Bleeding Tendency ◽  
Clinical Expertise ◽  
Diagnostic Features ◽  
Immunological Parameters

Background: Diagnosis of dengue hemorrhagic fever (DHF) in children is based on clinical suspicion and prompting laboratory criteria.Methods: This prospective study in children (6 months-14 years) presenting with features of DHF aimed to identify symptoms and signs, laboratory parameters for in early detection and assessed the association between clinical, laboratory, immunological parameters and outcome.Results: Of 50 children, 26 were females, mean±SD age was 8.3±3.02 years; age group 8-12 years were affected the most (38.0%) and infants the least (4.0%). Fever (100%), followed by skin rash (56.0%), myalgia (52.0%) and head ache (48.0%) were the symptoms. Fever of 4-5 days (52.0%), high grade fever (84.0%) and of sudden onset (76.0%) were common. Ascitis was seen in nine. Peteche and malena (48.0%) were predominant manifestation of bleeding tendency (100%). Positive tourniquet test (48.0%) did not correlate with bleeding tendency. Lower levels of Hemoglobin (<12gms%, 100%), hematocrit (<40%, 98.0%), platelet count (<100000/mm3, 100%), serum albumin (76.0%), and abnormal liver enzymes (64.0%) along with prolonged prothrombin time (44.0%) and aPTT (18.05%) were noted. Ultrasound abdomen confirmed Pleural effusion (52.0%), ascitis (22.0%), Hepatomegaly (68.0%). Mean detection time was 4 days. Positive dengue IgM and IgG (60.0%), IgM (16%), and IgG (24%) were observed with increase of IgM in early phase.Conclusions: lower platelet count, raising haematocrit, increased liver enzymes with low serum albumin levels are early indicators. Prolonged PT and APTT are associated with severe bleeding manifestations. Apart from clinical expertise, chest X-ray, abdominal ultrasonogram are useful diagnostic tools. 

scholarly journals Comparative assessment of laser therapy efficiency of chronic generalized periodontitis treatment

2020 ◽  
Vol 15 (3) ◽  
pp. 198-202
Author(s):  
Vladimir Akimov ◽  
◽  
Anna Fedoskina ◽  
Diana Kuzmina ◽  
Tatiana Vlasova ◽  
...  
Keyword(s):  
Secondary Prevention ◽  
Laser Therapy ◽  
Standard Treatment ◽  
Clinical Laboratory ◽  
Laboratory Data ◽  
Comparative Assessment ◽  
Functional Changes ◽  
Saint Petersburg ◽  
Improve Treatment ◽  
Standard Treatment Regimen

This paper is a result of collaboration between personnel of Saint-Petersburg and Saransk medical universities. The purpose of the investigation was to improve treatment and secondary prevention of chronic generelized periodontitis on the base of pathogenetically substantiated scheme of laser therapy. Cohort prospective study included 98 patients (31 men and 67 women) suffered from chronic generelized periodontitis at the age from 30 to 50 years with disease prescription from 3 to 10 years. All the patients were divided into three groups: standard anti-inflammatory therapy, laser therapy, antioxidant therapy. Efficiency of periodontitis treatment in the groups was estimated by clinical laboratory data, stomatological indices (РМА, SBI, API,) resistance of gingival capillaries; degree of osseous resorption. State of lipid peroxidation (LP) was estimated by the level of malondialdehyde (MDA, Fe2+-MDA) and phospholipase А2. It was found that application of laser and metabolic therapy considerably increase efficiency of standard treatment regimen and secondary prevention of chronic periodontitis, that is manifested in significant decrease structural-functional changes and in restoration of paradontium tissues microcirculation.

scholarly journals IMMUNOMODULATORY AND CLINICAL EFFECTIVITY OF THE DRUG "LAZOLEX" IN TREATMENT OF RECURRENT APHTHOUS STOMATITIS (RAS)

2021 ◽  
Author(s):  
MARIAM GOGOTISHVILI ◽  
NINO ABASHIDZE ◽  
NINO KORSANTIA ◽  
NATO KORSANTIA
Keyword(s):  
Immune System ◽  
Clinical Laboratory ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Patient Treatment ◽  
Clinical Activity ◽  
Dental Practice ◽  
Immunological Parameters ◽  
Traditional Therapy

The purpose of this study was to study the immunomodulatory and clinical properties of the Georgian drug “LAZOLEX” in patients with Recurrent Aphthous Stomatitis (RAS). Clinical and Immunological parameters were studied in 50 patients, the results showed the correlation between the condition of immune system and the severity of the RAS, i.e., the most severe immunosuppression was recorded in patients with severe stomatitis. 5% Gel of “LAZOLEX” compared to traditional therapy, showed the same high clinical activity, but the presence of immunocorrective properties in LAZOLEX manifested itself in an improvement in the quality of patient treatment: decrease of time of epithelization and a significant increase in the duration of remission. Conducted clinical, laboratory and immunological studies allow us to recommend LAZOLEX for active use in dental practice for RAS.

scholarly journals Clinical Laboratory and Epidemiological Features of Sudden Exanthema in Children Saint-Petersburg

2016 ◽  
Vol 15 (2) ◽  
pp. 5-9 ◽  
Author(s):  
E. V. Sharipova ◽  
I. V. Babachenko ◽  
Yu. I. Sichkovskaja ◽  
Yu. Yu. Chernyshova
Keyword(s):  
Clinical Laboratory ◽  
Saint Petersburg ◽  
Epidemiological Features

Flow Cytometric Analysis of Lymphocyte Subsets in Migraine Patients During and Outside of an Acute Headache Attack

Cephalalgia ◽  
1998 ◽  
Vol 18 (4) ◽  
pp. 197-201 ◽  
Author(s):  
AD Mosnaim ◽  
H Kulaga ◽  
AJ Adams ◽  
ME Wolf ◽  
J Puente ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Statistical Significance ◽  
Flow Cytometric Analysis ◽  
Immunological Parameters ◽  
Total N ◽  
Immune Parameters ◽  
Flow Cytometric ◽  
Group A ◽  
Group B ◽  
Group D

We have conducted flow cytometric studies of two subsets of lymphocyte markers in groups of migraineurs during ( n = 12; group B) and outside ( n = 10; group C) of a migraine without aura attack (total n = 22; group A), including a group of patients tested in both of these phases ( n = 5; group D), and compared these results with those obtained from a population of age-comparable, sex- and race-matched healthy volunteers ( n = 12; group E). Comparison of the first set of lymphocytes (CD3+CD16+56+, CD3-CD16+56+, CD3-CD19+, CD3+CD19+, and CD3+HLA-DR+) between the patients in group A and the controls (group E) showed differences, reflecting greater group A percentages of CD3+CD16+CD56+ and CD3-CD19+ lymphocytes. Furthermore, these differences reached statistical significance only for the CD3+CD16+CD56+ lymphocytes, and then solely for the patients in group C (Scheffe's test, p< 0.05). Paired analysis of the above lymphocyte markers for subjects in group D failed to show significant differences between patients when they were having and not having a migraine attack, raising the possibility that results from a larger study could show meaningful increases in percentages of CD3+CD16+CD56+ lymphocytes as one of the immune parameters useful for differentiating migraineurs from controls. Comparison of a second set of lymphocyte markers (CD19+CD5+, CD20+CD72-, CD20-CD72+, CD20+CD72+) among either the different groups of patients or between the patients and controls failed, however, to show statistically significant differences, emphasizing the apparent specificity of the findings described above for CD3+CD16+CD56+ lymphocytes. Our results, albeit of a preliminary nature, suggest the occurrence of significant, differential changes in lymphocyte subset immunophenotyping between groups of pain-free migraineurs and patients during an acute migraine episode or controls. Corroboration of these findings may prove useful in clinical laboratory practice to identify changes in immunological parameters specifically associated with migraineurs, and help towards a better understanding of the etiology and pathophysiology of this condition.

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