scholarly journals Research of Original Sorts of Honey

2021 ◽  
Vol 4 (1) ◽  
pp. 137-157
Author(s):  
Leonora Adamchuk ◽  
Natalia Dudchenko ◽  
Dina Lisohurska ◽  
Kateryna Pylypko
Keyword(s):  
Acetic Acid ◽  
Physicochemical Parameters ◽  
Glycolic Acid ◽  
Pollen Grains ◽  
Sugar Solution ◽  
Pollen Spectrum ◽  
Minor Components ◽  
Botanical Origin ◽  
Physicochemical Composition ◽  
Organoleptic Characteristics

The topicality. Honey, a natural saturated sugar solution, which consists of fructose and glucose in the amount of 65-80% of the total amount of sugars, and also contains important minor components – enzymes (diastase and invertase), organic acids (glycolic acid, acetic acid, etc.) , vitamins, phytoncides, phenolic compounds and minerals. Monofloral honeys, obtained mainly from the nectar of one plant species, are characterized by a conditionally stable composition and unique properties, which gives grounds to class them as functional products. Purpose and methods. The purpose of the work is to use melisopalinological, organoleptic and physicochemical analyses to determine reliable criteria for the authenticity of Ukrainian original sorts of honey. Results. The botanical origin of the investigated honeys has been confirmed by the study of the pollen spectrum of various honey samples by identifying the dominant pollen grains. In addition, all studied samples had their own profile of scent, tastes and consistency, a unique complex of which correlated with honey sorts. The varietal characteristics of the samples under study also influenced the physicochemical indicators, some values of which were outside the permissible limits in accordance with the national regulatory documentation. Conclusions and discussion. For original sorts of honey with varying degrees of monoflority, there is a need for further study of the physicochemical composition in connection with their possible varietal characteristics. The scientific novelty of the work lies in a pilot study of the organoleptic characteristics and physicochemical parameters of the original of honey from Ukraine, in particular pumpkin, blueberry, and echinacea. Practical use of the obtained results is possible for further authentication of coriander, goldenrod, pumpkin, blueberry, thyme, phacelia and Echinacea honey as original sorts for further study of their properties and introduction into functional nutrition.

scholarly journals IMPROVEMENT OF THE METHOD OF BOTANICAL IDENTIFICATION OF HONEY

2020 ◽  
Vol 14 (4) ◽  
Author(s):  
L. O. Adamchuk
Keyword(s):  
Pollen Analysis ◽  
Environmental Science ◽  
Pollen Grains ◽  
Food Product ◽  
Raw Material ◽  
Detection Methods ◽  
Pollen Spectrum ◽  
Botanical Origin ◽  
National University ◽  
Leading Enterprises

The pollen spectrum of honey reflects the regional crops, forest plantations, all the diversity of the plant species near the apiary. That is why the use, adaptation, and improvement of various methods of pollen analysis and interpretation of its results are topical questions in the countries which produce original varieties of honey on the international market. Ukraine is a leader in manufacturing and exporting honey in Europe and worldwide. Usually, Ukrainian honey is exported not as a finished food product, but as a raw material. This is because the pollen spectrum of Ukrainian honeys is provided with no description characterising their botanical and geographical origin, which, in turn, is because there are no pollen analysis methods implemented in  production laboratories. The purpose of this study was improving the pollen analysis method to identify the botanical origin of Ukrainian honeys. The research was conducted in the laboratory of the Department of Standardisation and Certification of Agricultural Products in the National University of Life and Environmental Science of Ukraine, and in the production laboratory of Ascania-Pack Company. The analysis of harmonised methods of melissopalynology and standardised pollen detection methods has allowed developing a botanical method of honey identification adapted to be introduced in Ukrainian laboratories. The method suggested is a complex of individual procedures, operations,  and techniques. It has been detailed how to prepare solution to fix and stain pollen preparations obtained from honey. The method of preparing a suspension of pollen grains from honey has been improved: extra dissolution of crystals and a lower speed of the centrifuge reduce the time of making the preparation and improve its quality. The method of obtaining preparations of pollen grains from honey has been adapted to harmonised international melissopalynological methods. New methods have been developed to count pollen grains in prepared monofloral and polyfloral honey samples. It has been detailed how to identify step-by- step the botanical origin of honey by pollen grains using international databases. The method has been implemented in the production laboratories of leading enterprises in Ukraine and Belarus.

scholarly journals Sensorial, Melissopalynological and Physico-Chemical Characteristics of Honey from Babors Kabylia’s Region (Algeria)

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 225
Author(s):  
Asma Ghorab ◽  
María Shantal Rodríguez-Flores ◽  
Rifka Nakib ◽  
Olga Escuredo ◽  
Latifa Haderbache ◽  
...  
Keyword(s):  
Sensory Properties ◽  
Chemical Components ◽  
Chemical Characteristics ◽  
Pollen Spectrum ◽  
Botanical Origin ◽  
Flavonoid Content ◽  
Chemical Parameters ◽  
High Tendency ◽  
Physico Chemical ◽  
Pollen Types

This study aimed to characterize the honeys of Babors Kabylia through sensory, melissopalynological and physico-chemical parameters. Thirty samples of honey produced in this region were collected over a period of two years and analyzed. All the samples presented physico-chemical parameters in conformity with legislation on honey quality, with few exceptions, linked mainly to beekeeping management. The pollen spectrum revealed a great diversity with 96 pollen types. The main pollen types were spontaneous species as Fabaceae (Hedysarum, Trifolium, Genisteae plants), Asteraceae plants, Ericaceae (Erica arborea L.) or Myrtus and Pistacia. The sensory properties of samples showed a high tendency to crystallization, the colors were from white to brown, but most of them had gold color. Smell and odor corresponded mainly to vegetal and fruity families and in taste perceptions besides sweetness highlighted sourness and saltiness notes. Seventeen samples were polyfloral, one was from honeydew and twelve were monofloral from heather, genista plants, sulla, blackberry or Asteraceae. Heather and the honeydew samples showed the darkest color, the highest electrical conductivity and phenol and flavonoid content. A statistical analysis based on the most representative pollen types, sensory properties and some physico-chemical components allowed the differentiation of honey samples in terms of botanical origin.

Botanical origin discrimination of Greek honeys: physicochemical parameters versus Raman spectroscopy

2020 ◽  
Author(s):  
Marinos Xagoraris ◽  
Elisavet Lazarou ◽  
Eleftheria H Kaparakou ◽  
Eleftherios Alissandrakis ◽  
Petros A Tarantilis ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Physicochemical Parameters ◽  
Botanical Origin

scholarly journals Using palynological evidence from royal jelly to mediate the spread of Paenibacillus larvae in Brazil

Hoehnea ◽  
2018 ◽  
Vol 45 (3) ◽  
pp. 512-539 ◽  
Author(s):  
Cynthia Fernandes Pinto da Luz ◽  
Lubiane Guimarães-Cestaro ◽  
José Eduardo Serrão ◽  
Dejair Message ◽  
Marta Fonseca Martins ◽  
...  
Keyword(s):  
Royal Jelly ◽  
Pollen Grains ◽  
Paenibacillus Larvae ◽  
Inspection System ◽  
American Foulbrood ◽  
Pollen Spectrum ◽  
Paulo State ◽  
Total Percentage ◽  
Brazilian Legislation ◽  
American Foulbrood Disease

ABSTRACT Royal jelly may contain pollen grains and their presence can be used to determine the phytogeographical origin of the product. This study analyzed the phytogeographical origin of commercial royal jelly samples from São Paulo State, tested as part of the Brazilian Federal Inspection System (SIF), found to be contaminated with spores of the bacterium Paenibacillus larvae, that causes the American Foulbrood Disease. The pollen grains of Castanea had the highest total percentage, with lower percentages of Cirsium/Carduus, Cistus, Parthenocissus, Prunus, Quercus, Robinia, Scrophulariaceae, Taraxacum, Tilia, among others. This pollen spectrum is incompatible with royal jelly samples produced in Brazil. The pollen spectrum resembled that of an imported product, compatible with the Northern Hemisphere origin. Brazilian legislation does not require the phytogeographic origin of imported bee products to be analyzed by palynological procedures, but it is mandatory to have a certificate issued by the country of origin attesting the absence of pathogens, monitored with the objective of preventing the exotic diseases from entering Brazil. Palynology, therefore, proved to be fundamental in detecting imports of this contaminated batches.

scholarly journals Quality Assessment of Goldenrod, Milkweed and Multifloral Honeys Based on Botanical Origin, Antioxidant Capacity and Mineral Content

2022 ◽  
Vol 23 (2) ◽  
pp. 769
Author(s):  
Marianna Kocsis ◽  
Alexandra Bodó ◽  
Tamás Kőszegi ◽  
Rita Csepregi ◽  
Rita Filep ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Mineral Content ◽  
Principal Component ◽  
Pollen Spectrum ◽  
Botanical Origin ◽  
Multivariate Statistical ◽  
Zn Content ◽  
Five Elements ◽  
Multifloral Honey ◽  
Melissopalynological Analysis

The goal of the study was to evaluate the pollen spectrum, antioxidant capacity and mineral content of four Hungarian honey types, using multivariate statistical analysis. The light colored honeys were represented by milkweed honey and a multifloral (MF) honey with dominant pollen frequency of linden (MF-Tilia); the darker ones were goldenrod honey and a multifloral honey with Lamiaceae pollen majority (MF-Lamiaceae). The pollen spectrum of the samples was established with melissopalynological analysis. The absorbance of the honeys positively correlated with the antioxidant capacity determined with three of the used methods (TRC, TEAC, DPPH), but not with ORAC. The latter method correlated negatively also with other antioxidant methods and with most of the mineral values. MF-Tilia had high ORAC value, K and Na content. The MF-Lamiaceae had the highest K, Mg, P, S, Cu and Zn content, the last five elements showing strict correlation with the TRC method. The darker goldenrod honey had higher SET values and total mineral content, than the milkweed honey. The above character-sets facilitate identification of each honey type and serve as indicators of variety. The antioxidant levels and mineral content of honeys allowed their clear separation by principal component analysis (PCA).

scholarly journals Modulation of Auxin Levels in Pollen Grains Affects Stamen Development and Anther Dehiscence in Arabidopsis

2018 ◽  
Vol 19 (9) ◽  
pp. 2480 ◽  
Author(s):  
Hernán Salinas-Grenet ◽  
Ariel Herrera-Vásquez ◽  
Samuel Parra ◽  
Allan Cortez ◽  
Lilian Gutiérrez ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Apical Meristem ◽  
Seed Set ◽  
Transgenic Arabidopsis ◽  
Pollen Grains ◽  
Anther Development ◽  
Anther Dehiscence ◽  
Transgenic Lines ◽  
Indole 3 Acetic Acid ◽  
Auxin Accumulation

Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-l-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development.

scholarly journals Morphology, physicochemical properties and antioxidant capacity of bee pollens

2019 ◽  
Vol 37 (No. 1) ◽  
pp. 1-8 ◽  
Author(s):  
Roman Bleha ◽  
Tatiana Shevtsova ◽  
Andrej Sinica ◽  
Vojtech Kruzik ◽  
Jan Brindza
Keyword(s):  
Physicochemical Properties ◽  
Antioxidant Capacity ◽  
Ftir Spectroscopy ◽  
Water Activity ◽  
Antioxidant Properties ◽  
Pollen Grains ◽  
Botanical Origin ◽  
Colour Parameters

Six supposedly unifloral bee pollens of various botanical origins were characterised by morphometry, SEM, CIE L*a*b* colour parameters and FTIR spectroscopy. Botanical origin and homogeneity of bee pollens were verified by colour and morphology of pollen grains. Water activity, moisture and antioxidant capacity of bee pollens were also evaluated. The results were discussed in terms of connection between botanical origin, composition and antioxidant properties of pollen materials.

scholarly journals New sedimentological and palynological data from surface Miocene strata in the central Amazonas Basin area

2015 ◽  
Vol 45 (3) ◽  
pp. 337-357 ◽  
Author(s):  
Emílio Alberto Amaral Soares ◽  
Rodolfo Dino ◽  
Dominicky Peres Soares ◽  
Luzia Antonioli ◽  
Marco Antônio Lima da Silva
Keyword(s):  
Geographic Distribution ◽  
Amazon Basin ◽  
Middle Miocene ◽  
Pollen Grains ◽  
Minor Components ◽  
Point Bar ◽  
Palynological Data ◽  
Continental Origin ◽  
Crevasse Splay ◽  
Basin Area

ABSTRACT The scarcity of stratigraphic data has hindered the demarcation of the outcropping area of Miocene deposits of the Amazon Basin, represented informally by the Novo Remanso Formation. Moreover, this unit is characterized by a sparse and irregular geographic distribution due to its sedimentological features and rare fossil content. Miocene deposits cropping out in central Amazonas Basin area were described in sedimentological terms and analyzed palynologically. All analyses were undertaken in samples collected at the Uatumã River banks (Itapiranga and São Sebastião do Uatumã cities). Lithostratigraphic data shows that Novo Remanso Formation consists of sandstones, with subordinate conglomerates and pelites, characteristic of a meandering fluvial paleosystem, with fluvial channel, point bar, floodplain and crevasse splay facies. The palynoflora retrieved from five samples consists exclusively of continental-origin palynomorphs dominated by angiosperms species. Trilete spores are well represented, while gymnosperms pollen grains are minor components. The presence of Psilastephanoporites tesseroporus, Syncolporites poricostatus, Jandufouria seamrogiformis and Polypodiaceoisporites potoniei ensure these deposits fits into the Grimsdalea magnaclavata palynozone (Regali et al. 1974a, b), and the Grimsdalea magnaclavata/Crassoretitriletes vanraadshooveni palynozones of Jaramillo et al. (2011) considered Middle Miocene age. This age is confirmed by the zonation of Jaramillo et al. (2011), based on the LADs of Bombacacidites baumfalki (11.57Ma) and Crototricolpites annemariae (12.91Ma); and the FAD of Psilastephanoporites tesseroporus (14.00Ma). With these new data presented herein, it is possible to assume that the Miocene strata represented by the Novo Remanso Formation covers a larger area in the basin than previously considered, and that it may be extended for about 300 km until the Manacapuru village, indicating a Miocene subsidence phase.

Pollen- and Non Pollen Palynomorphs- Analyses from Svalbard

2017 ◽  
Vol 7 (2) ◽  
pp. 123-132
Author(s):  
Vlasta Jankovská
Keyword(s):  
Pollen Grains ◽  
The Arctic ◽  
Pollen Spectrum ◽  
Pollen And Spores

We analysed fifteen samples of a shallow profile “Sverrefjellet Volcano “, located in the NW part of Svalbard – Bockfjord region (see Fig.1). Frequency of pollen grains, spores and NPP (Non Pollen Palynomorphs) was quite low. Only the local taxa (e.g. Salix and Cyperaceae) produced pollen in greater abundance. The pollen spectrum was considerably varied, although pollen grains and spores of most taxa were present in low abundances. This was due to low pollen and spores productivity of the Arctic flora, which propagates mostly in a vegetative way. To obtain as much information from the profile under study as possible, we analysed the non- pollen palynomorphs as well. We succeeded above all in determination of eggs, buccal tubes and claws of the Tardigrada phylum (Jankovská 1991). The determination has been recently verified by specialists in the field, prof. L. Kaczmarek and dr. M. Roszkowska (in Jankovská et al. 2016). The results of the pollen- and NPP analysis are presented in pollen- and NPP diagram, which represents a picture of the species-poor Arctic flora.

scholarly journals Pollen morphology of Myrtaceae visited by social bees

2019 ◽  
Vol 6 (2) ◽  
pp. 98-105
Author(s):  
Andreia Santos do Nascimento ◽  
Carlos Alfredo Lopes de Carvalho
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Morphological Characters ◽  
Medium Size ◽  
Morphological Description ◽  
Pollen Spectrum ◽  
Social Bees ◽  
Equatorial Diameter ◽  
Polar Diameter

This study aimed to characterize species of Myrtaceae belonging to genera already identified in the pollen spectrum of beehive products of social bees, describing pollen grains morphology and correlating with taxonomic differences the species reported in the literature. For each plant species, measurements were made with 25 pollen grains in equatorial view and the polar diameter (PD) and equatorial diameter (ED) were measured. The exine thickness was obtained by measuring 10 pollen grains in polar view at the medium height of mesocolpium. The description of pollen morphology showed that pollen grains of the species are triangular amb, 3-colporates, psilate surface, small to medium size and oblate shape with the P/E ratio ranging from 0.52 to 0.72 µm. The morphological description of Myrtaceae species showed similarity between several characteristics common to pollen grains of this species. The description also allowed separation of nine species studied by size and specific morphological characters with the identification key, which can also be used to study the pollen spectrum of hive products.

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