scholarly journals Hepatotropic, antioxidant and antitoxic action of amaranth oil

2014 ◽  
Vol 4 (5) ◽  
pp. 159 ◽  
Author(s):  
V. A. Nikolaevsky ◽  
D. M. Martirosyan ◽  
E. N. Muzalevskaya ◽  
L. A. Miroshnichenko ◽  
V. I. Zoloedov
Keyword(s):  
Antioxidant Activity ◽  
Free Radical Oxidation ◽  
Hepatoprotective Activity ◽  
Morphological Examination ◽  
Drug Induced ◽  
Pressing Method ◽  
Radical Oxidation ◽  
Amaranth Oil

Background: It is our opinion that amaranth seed oil’s properties merit further study due to their high quantity of squalene. Squalene has been shown to have antitoxic, antioxidant, and hepatotropic properties. The liver is one of the most important organs in the human body, and as a filtering organ, it is subjected to many toxic compounds and free radicals. Thus, protecting the liver with an antioxidant and antitoxic compound could offer significant benefits.Objective: In the present study we have evaluated the antitoxic, antioxidant, and hepatotropic (hepatoprotective) effects of amaranth oil acquired via a patented pressing method. Rats were treated with amaranth oil, a comparative compound, or a control compound and then subjected to various toxic, oxidizing, and other compounds in order to examine amaranth oil’s effects. Results: Amaranth oil was found to have a positive and dose-dependent effect both in vivo and in vitro. Amaranth oil’s hepatoprotective activity was confirmed by both biological and morphological examination. This study suggests that amaranth oil acts to prevent and reduce CT damage in liver tissue. This was especially the case in doses of 300 mg/kg, proved both by normalization of hepatic ferments-markers indexes and morphological examinations.Conclusion: The amaranth oil acquired via a patented pressing method possesses antitoxic and antioxidant activity.  In this connection, the amaranth oil can be recommended for prophylaxis of toxic and drug-induced liver lesions and as a component of the functional food and dietetics of various diseases.Keywords: antioxidant activity, free-radical oxidation, hypoxia, erythrocytes hemolysis, squalene, amaranth oil, hepatoprotector, hexenalum sleep, toxic hepatitis 

scholarly journals Antimicrobial and Hepatoprotective Potential of Pterospermum acerifollium leaves Extracts on Swiss albino mice

2020 ◽  
Vol 10 (3) ◽  
pp. 170-174
Author(s):  
Deepa Varandani ◽  
Sharmishta Sekhar ◽  
Suman Trivedi ◽  
Megha Jha ◽  
Sourabh Jain
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Hepatoprotective Activity ◽  
Diffusion Method ◽  
Antitubercular Drug ◽  
Drug Induced ◽  
Swiss Albino Mice ◽  
Serum Biochemical ◽  
Albino Mice

The objective of present study to investigate the hepatoprotective activity of hydro-alcohollic extract leaves of Pterospermum acerifollium against antitubercular drug induced liver damage in swiss albino mice and also performs antimicrobial activity by disc diffusion assay. Successive extractions was performed with different organic solvents viz; hydroalcohollic by cold maceration. The extract was analysed as antioxidant activity as a content of Total phenolic content, Total flavanoid content, Reducing power assay and DPPH Scavenging assay. Antimicrobial activity of methanolic extract was estimated by Agar well diffusion method. Antitubercular drug induced is used as toxicants in hepatoprotective studies in acute condition was analysed by serum biochemical estimations by AST, ALT, ALP and Total Bilirubin. In-vivo Antioxidant activity was performed by LPO, GSH, SOD and Catalase. During the collection of tissue for biochemical estimation piece of tissue cut and transferred for Histopathological estimation. The levels were measured and it indicated that the extract had significant antioxidant activity however the results obtained were dose dependent the higher the dose (400 mg/kg) the better activity. The extract administered at dose 400 mg/kg showed better activity. The treatment with hydroalcohollic extract of Pterospermum acerifillium reduced the elevated levels of SGOT, SGPT, ALP, TB and also reversed the hepatic damage towards normal which further supports the hepatoprotective activity. Keywords: Succesive extraction, In-vivo, Serum biochemical, Cold maceration

scholarly journals EVALUATION OF ANTIOXIDANT ACTIVITY OF PLANT AGENTS

2020 ◽  
pp. 187-193
Author(s):  
Yekaterina Vladimirovna Ferubko ◽  
Valeriy Nikolayevich Zelenkov ◽  
Anatoliy Andreyevich Lapin ◽  
Tamara Darizhapovna Dargayeva
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Lipid Analysis ◽  
Free Radical Oxidation ◽  
Inhibitory Effect ◽  
Total Antioxidant Activity ◽  
Radical Oxidation ◽  
White Rats ◽  
Total Antioxidant

The aim of the research is to determine the total antioxidant activity of in vitro collection of antihepatotoxic action consisting of roots and rhizomes of Inula helenium L., grass Centaurium erythraea Rafn., flowers Tanacetum vulgare L., fruits Rosa sp., fruits of Crataegus sp. аnd its separate components by the method of colonometric titration and determination of antioxidant activity of dry extract (conditional name "Pentafite") obtained from this collection under conditions of experimental tetrachloride of carbon hepatitis in white rats. A method of chemiluminescent lipid analysis was used to evaluate the free radical oxidation of lipids in liver in test animals. It has been found that collection of antihepatotoxic action and plants included in it have antioxidant activity in vitro, at the same time synergistic effect on manifestation of total antioxidant activity of collection is revealed. Pharmacotherapy of experimental hepatitis in experimental rats by administration of Pentafite at a dose of 300 mg/kg has been found to significantly reduce the chemiluminometric values of lipids, which characterize the intensity of free radical reactions. "Pentafite" has a pronounced inhibitory effect on hyperlipoperoxidation in animal liver in case of toxic damage to it. The established antioxidant activity of collecting antihepatotoxic action and Pentafite extract may be important for their use in the treatment and prevention of hepatobiliary diseases.

scholarly journals In-vivo Hepatoprotective and Antioxidant Activities of Sphaeranthus amaranthoides Burm.f. Against Anti-Tubercular Drugs Induced Hepatotoxicity in Rats

2021 ◽  
pp. 15-23
Author(s):  
M. R. Vinayakamurthi ◽  
J. Anbu Jeba Sunilson ◽  
A. V. Anita Gnana Kumari ◽  
U. Aathilakshmi
Keyword(s):  
Antioxidant Activity ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Liver Homogenate ◽  
Hepatoprotective Activity ◽  
Thiobarbituric Acid Reactive Substance ◽  
Enzymatic Antioxidants ◽  
Drug Induced

Aim: To evaluate the hepatoprotective and antioxidant activity of Sphaeranthus amaranthoides Burm.f. against isoniazid (INH) and rifampicin (RIF) induced hepatotoxicity in rats. Study Design: Experimental study. Place and Duration: Research lab, Department of Siddha Medicine, Tamil University, Thanjavur, India, between March 2018 and November 2019. Methodology: Liver toxicity was induced by antitubercular drugs (Isoniazid; INH+Rifampicin; RIF) at a dose level of 50+100 mg/kg each, p.o for 15 days. Petroleum ether, Chloroform, Methanol, Aqueous extracts of Sphaeranthus amaranthoides Burm.f. (S. amaranthoides) (200 and 400 mg/kg bt.wt.) were administered orally once daily for 15 days. The hepatoprotective activity was assessed using various biochemical parameters SGOT, SGPT, ALP, bilirubin, total protein, albumin, total cholesterol, total bilirubin, direct bilirubin and LDH. The antioxidant activities such as the enzymatic activity of superoxide dismutase (SOD), and catalase (CAT), and the level of lipidperoxidation as thiobarbituric acid reactive substance (TBA-RS) were measured in liver homogenates and histological examinations were carried out to assess hepatoprotective activity. For Statistical analysis, the values were subjected to one way analysis of variance (ANOVA) followed by Tukey multiple compare test. Results were considered statistically significant when P<0.05. Results: The treatment with methanolic extract (400 mg/kg bt.wt.) of S. amaranthoides significantly prevented drug-induced increase in serum levels of liver enzymes (P<0.001). The antioxidant activity of a dose of 400 mg/kg of S. amaranthoides significantly prevented the decreases in the activity of enzymatic antioxidants (CAT & SOD) (P<0.01 and P<0.001) and inhibited the elevation of lipid peroxidation (TBA-RS) in the liver homogenate. Histopathology of liver tissue showed that S. amaranthoides attenuated the hepatocellular necrosis, regeneration and repair of cells toward normal. Conclusion: The methanolic extract of S. amaranthoides showed significant hepatoprotectivity and antioxidant activity against INH + RIF Anti TB drugs.

scholarly journals Hepatoprotective activity of methanolic extract of young shoots of Bambusa arundinaceae in thioacetamide induced liver injury in rats

2017 ◽  
Vol 6 (2) ◽  
pp. 140-148
Author(s):  
Kajal N Chauhan ◽  
◽  
Bhavini Shah ◽  
Manish Nivsarkar ◽  
◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liver Injury ◽  
Cell Viability ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Tumor Cell Line ◽  
Hepatoprotective Activity

Background: Whole plant of Bambusa arundinaceae (Bamboo) belonging to family (Graminae) is used in number of disease including liver ailments in various parts of India. The present study is done to evaluate antioxidant and Hepatoprotective activity of methanolic extract of young shoots of Bambusa arundinaceae in Thioacetamide induced liver injury in rats. Material and method: Antioxidant activity of methanolic extract was evaluated by DPPH, Ferric reducing power assay and Lipid peroxidation. In vitro hepatoprotective activity of methanolic extracts (25, 50, 100, 200 and 400 µg/ml) against thioacetamide (100mM) induced cytotoxicity was assessed by monitoring cell viability in HepG2 and Hep3b tumor cell line and also in primary hepatocytes. In vivo hepatoprotective activity of methanolic extract (50, 100, 200 mg/kg p.o.) was observed againt thioacetamide (100 mg/kg s.c.) induced liver injury in rat by measuring biochemical parameters viz, Aspartate Transaminase, Alanine Transaminase, Alkaline Phosphatase, Total and Direct Bilirubin. The histopathological studies were also performed. Results: Methanolic extract of young shoots showed good antioxidant activity. Thioacetamide caused significant reduction in cell viability in in vitro studies. Treatment with methanolic extract caused significant increase in cell viability. In vivo studies showed that Thioacetamide induced elevation of biochemical parameters in rats were significantly (p<0.0001) decreased with methanolic extract treatment in rats. Histopathology studies also supported the protective effect of methanolic extract. Conclusion: The current study reveals that the methanolic extract of Bambusa arundinaceae has hepatoprotective activity which may be due to its antioxidant activity.

Antioxidant Activity, Phenolic Content and Hematoprotective Effects of Cleome arabica Polyphenolic Leaf Extract

2019 ◽  
Author(s):  
C. Tigrine ◽  
A. Kameli
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Reducing Power ◽  
Hematological Toxicity ◽  
Protective Effects ◽  
Ferrous Ions ◽  
Polyphenolic Extract ◽  
Cleome Arabica

In this study a polyphenolic extract from Cleome arabica leaves (CALE) was investigated for its antioxidant activity in vitro using DPPH•, metal chelating and reducing power methods and for its protective effects against AraC-induced hematological toxicity in vivo using Balb C mice. Results indicated that CALE exhibited a strong and dose-dependent scavenging activity against the DPPH• free radical (IC50 = 4.88 μg/ml) and a high reducing power activity (EC50 = 4.85 μg/ml). Furthermore, it showed a good chelating effects against ferrous ions (IC50 = 377.75 μg/ml). The analysis of blood showed that subcutaneous injection of AraC (50 mg/kg) to mice during three consecutive days caused a significant myelosupression (P < 0.05). The combination of CALE and AraC protected blood cells from a veritable toxicity. Where, the number of the red cells, the amount of hemoglobin and the percentage of the hematocrite were significantly high. On the other hand, AraC cause an elevation of body temperature (39 °C) in mice. However, the temperature of the group treated with CALE and AraC remained normal and did not exceed 37.5 °C. The observed biological effects of CALE, in vitro as well as in vivo, could be due to the high polyphenol and flavonoid contents. In addition, the antioxidant activity of CALE suggested to be responsible for its hematoprotective effect.

A Review on Melatonin’s Effects in Cancer: Potential Mechanisms

2018 ◽  
Vol 18 (7) ◽  
pp. 985-992 ◽  
Author(s):  
Aysegul Hanikoglu ◽  
Ertan Kucuksayan ◽  
Rana Cagla Akduman ◽  
Tomris Ozben
Keyword(s):  
Systematic Review ◽  
Antioxidant Activity ◽  
Membrane Receptors ◽  
Cancer Development ◽  
Secretory Product ◽  
Prevention And Treatment ◽  
G Protein Coupled

This systematic review aims to elucidate the role of melatonin (N-acetyl-5-metoxy-tryptamine) (MLT) in the prevention and treatment of cancer. MLT is a pineal gland secretory product, an evolutionarily highly conserved molecule; it is also an antioxidant and an impressive protector of mitochondrial bioenergetic activity. MLT is characterized by an ample range of activities, modulating the physiology and molecular biology of the cell. Its physiological functions relate principally to the interaction of G Protein-Coupled MT1 and MT2 trans-membrane receptors (GPCRs), a family of guanidine triphosphate binding proteins. MLT has been demonstrated to suppress the growth of various tumours both, in vivo and in vitro. In this review, we analyze in depth, the antioxidant activity of melatonin, aiming to illustrate the cancer treatment potential of the molecule, by limiting or reversing the changes occurring during cancer development and growth.

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