scholarly journals THE EFFECT OF NIFEDIPINE ON SURVIVE CELL, APOPTOTIC CELL AND NECROTIC CELL OF IPSILATERAL TESTICULAR GERMINAL EPITELIAL CELLS ON MALE WISTAR RATS WITH UNILATERAL TESTICULAR TORSION

2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Andrie Rhomdhon Kurniawan ◽  
Lukman Hakim ◽  
Doddy M Soebadi
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Testicular Torsion ◽  
Intraperitoneal Administration ◽  
Positive Control ◽  
Lower Number ◽  
Positive Control Group ◽  
Control Group ◽  
P Value ◽  
Male Wistar Rats

Objective: This study aimed to compare the number of survival, apoptotic and necrotic cells of ipsilateral testicular germinal epithelial cells in male wistar rats with unilateral testicular torsion between nifedipine given and control groups. Material & Methods: Thirty male wistar rats aged 10-12 weeks were randomly divided into 5 groups, each consisted of 6 rats. The negative control group (KN) underwent a sham procedure and left orchidectomy. Positive control group 4 (KP4) and 10 (KP10) performed left torsio testis 3 x 360 degrees medially for 4 hours and 10 hours respectively, then performed orchidectomy 4 hours after detorsion. The 4-hour (N4) and 10 hours (N10) nifedipine treatment group received the same treatment with positive control, but 30 min before detorsion performed, nifedipine were given intraperitoneal 100μg/kg. Within 1 hour after orchidectomy, cell count was calculated using flow cytometry. Results: It was found that the 4 (N4) and 10 hours (N10) nifedipine treatment group had a higher survival cells and also a lower number of apoptotic and necrotic cells compared to the positive control group. It was found that the 10 hours nifedipine treatment group (N10) had a lower number of apoptotic and necrotic cells compared to the 10 hour positive control group (KP10). The difference was statistically significant with p value <0.05. However, in KP4 and N4 group compared with KP10 and N10 group, higher apoptotic cells was obtained. This was a new phenomenon that needs to be investigated more deeply. Conclusion: Intraperitoneal administration of nifedipine prior to testicular detorsion may reduce the number of apoptotic and necrotic cells of testicular germinal epithelial cell, and may increase the number of survival cells in ipsilateral testes with unilateral testicular torsion.

scholarly journals PENGARUH PEMBERIAN GEL AIR KELAPA GADING (COCOS CAPITATA) TERHADAP TINGKAT KECEMASAN PADA HIPERSENSITIVITAS DENTIN Studi in vivo terhadap tikus wistar jantan

2016 ◽  
Vol 3 (2) ◽  
pp. 133
Author(s):  
Chariza Hanum Mayvita Iskandar ◽  
Yayun Siti Rochmah ◽  
Arlina Nurhapsari
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Positive Control ◽  
Anxiety Level ◽  
Positive Control Group ◽  
Control Group ◽  
Dentin Hypersensitivity ◽  
Group I ◽  
Significant Difference

Background: Ivory palm water (Cocos capitata) has a high potassium content. Potassium ion can depolarized excited dental nerve on dentin hypersensitivity. This study was to determine the effect of water gel ivory palm against dentine hypersensitivity by the anxiety level of mice using RGS (Rat Grimace Scale).Method: This research has been through ethical clearance which researchmethod was quasy experimental in vivo. The sample was 16 Wistar rats (Rattus novergicus) male 4-5 months with 4 mice in each group. Group I as a positive control. Group II as the ivory palm water gel 10% treatment group. Group III as the ivory palm water gel 70% treatment group. Group IV as a negative control. Research data analysis used Kruskal Wallis test with p <0.05 then continued by Mann Whitney test.Result: Kruskal Wallis test obtained significant difference between the four study groups (p <0.05). Mann Whitney test results in comparison either between positive control group and ivory palm water gel 10% and 70%, and comparison group of ivory palm water gel 10% and 70% weren’t significantly different.Conclusion: This study conclusion there was no significant difference between ivory palm water gel 10 % and 70 % in reducing anxiety level in male Wistar rats with dentinal hypersensitivity

scholarly journals Uji efektivitas ekstrak daun sendok (Plantago major L.) terhadap waktu perdarahan pada tikus Wistar jantan (Rattus norvegicus)

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Abedneju R. Kainde ◽  
Damajanty H.C. Pangemanan ◽  
Bernart S.P. Hutagalung
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Bleeding Time ◽  
Wistar Rat ◽  
Control Group ◽  
P Value ◽  
Plantago Major ◽  
Control Group Design ◽  
Group Design ◽  
Male Wistar Rats

Abstract: Herbal plant is often used to treat any kind of disease. There are many kinds of plants in Minahasa used as traditional medicine, inter alia daun sendok (Plantago major L.). Daun sendok is often used to treat bleeding. This study was aimed to obtain the effectiveness of daun sendok extract on bleeding time in male Wistar rats. This was a true experimental study with a posttest only group design. This study was conducted at the Laboratory of Pharmacology Faculty of Medicine University of Sam Ratulangi. Total samples were 14 rats divided into 2 groups: control group and treatment group. Wounds of 2 mm depth were perfomed on the rat tails of the treatment group followed by application of daun sendok extract. The results showed that the average bleeding time of the control group was 51.87 seconds meanwhile of the treatment group was 29.67 seconds. The unpaired t-test between the two groups showed a p value of 0.000. Conclusion: Daun sendok (Plantago major L.) extract could reduce the bleeding time in male Wistar rats. Keywords: bleeding time, daun sendok, Wistar rat Abstrak: Tanaman obat seringkali digunakan untuk mengobati berbagai macam jenis penyakit. Di wilayah Minahasa terdapat banyak jenis tanaman yang digunakan sebagai obat tradisional, salah satunya ialah daun sendok (Plantago major L.) yang seringkali digunakan untuk mengobati perdarahan. Penelitian ini berrtujuan untuk mengetahui efektivitas ekstrak daun sendok terhadap waktu perdarahan tikus Wistar jantan. Jenis penelitian ialah true experimental dengan post test only control group design yang dilakukan di Laboratorium Farmakologi Fakultas Kedokteran Unsrat dengan total sampel berjumlah 14 ekor tikus Wistar yang dibagi menjadi 2 kelompok yaitu: kelompok kontrol dan kelompok perlakuan menggunakan ekstrak daun sendok. Pada kelompok uji diaplikasikan ekstrak daun sendok setelah dilakukan perlukaan di bagian ekor tikus sedalam 2 mm. Hasil penelitian memperlihatkan rerata waktu perdarahan pada kelompok kontrol ialah 51,87 detik sedangkan pada kelompok perlakuan 29,67 detik. hasil uji t tidak berpasangan antara kedua kelompok mendapatkan nilai p=0,000. Simpulan: Ekstrak daun sendok (Plantago major L.) efektif dalam memperpendek waktu perdarahan pada tikus Wistar.Kata kunci: waktu perdarahan, daun sendok, tikus Wistar

scholarly journals EFEK EKSTRAK MELON (Cucumis melo) dan GliadinTERHADAP KADAR Hb DAN HbCO TIKUS WISTAR JANTAN YANG DIPAPAR ASAP ROKOK

2017 ◽  
Vol 11 (1) ◽  
pp. 78
Author(s):  
Yuyun Erlina Susanti ◽  
Bambang Wirjatmadi
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Cucumis Melo ◽  
Wistar Rats ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

The exposure of cigarette smoke produce CO bond in hemoglobin. Melon (Cucumis melo) and gliadin contains of antioxidants that prevent tissue damage. The aim of this study was to analyze the difference of hemoglobin and carboxyhemoglobin concentration in male Wistar rats were exposed  to cigarette smoke and had been treated melon extract (Cucumis melo) and gliadin. The study was a laboratory experimental design, using Post Test Control Group Design Research and RAL method (Complete Randomize Design). The sample in this study were 25 male Wistar rats aged 3 months. The research was divided into 5 groups with 5 different treatment : control group, the treatment group were given exposure to cigarette smoke, the treatment group were given of cigarette smoke exposure and melon extract (Cucumis melo) and gliadin dose of 3 IU / day, 4.5 IU / day, and 9 IU / day.The subject were divided into 5 groups each treated for 28 day. The sample size used Federer formula. The collection of data was obtained from the results of laboratory tests to hemoglobin and Carboxyhemoglobin. Hb and HbCO data collected and analyzed by Manova test at 95% confidence level. The results showed significant difference in average Hb and HbCO concentration (p-value = 0.000) between male Wistar rats treated and not treated with melon extract (Cucumis melo) and gliadin. Melon extract has an effect on the decline of HbCO concentration due to exposure to cigarette smoke.Keywords:smoke, melon extract, Hb, HbCO

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Effectiveness of Boiled Figs (Ficus carica L), Rosy Periwinkle (Catharanthus roseus) and Cherry (Muntingia Calabura L.) on Decreasing SGOT and SGPT Serum in Male Wistar Strain Rats with Acute Hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 744-755
Author(s):  
Grace Arni Yunita Hutajulu ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Catharanthus Roseus ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group ◽  
Wistar Strain ◽  
Muntingia Calabura

Acute hepatitis is inflammation of the liver. This disorder is usually caused by a virus or exposure to alcohol, drugs, toxic, or other pathogens. The purpose of this study was to determine the effectiveness of boiled figs leaves (Ficus carica L.), Rosy Periwinkle (Catharanthus roseus), and cherry (Muntingia Calabura L.) on decreasing serum levels of SGOT and SGPT. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. Rats were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 0.3 grams Figs (Ficus carica L.), 3.5 grams of rosy Periwinkle (Catharanthus roseus), and 3.5 grams of cherry (Muntingia Calabura L.) was given as much as 3.6 cc orally for 7 days to treatment group. Data were analyzed with SPSS version 24, One Way ANOVA test was performed to compare SGOT and SGPT levels. The results showed a significant difference in SGOT levels between the treatment group, positive control group, and negative control group (p <0.05) and there were significant differences in SGPT levels between the treatment group and positive control group (p <0.05). The conclusions of this study were mixed  boiled of Figs (Ficus carica L.), rosy Periwinkle (Catharanthus roseus), and and Cherry (Muntingia Calabura L.) had an effect in decreasing serum SGOT and SGPT in male rats with acute hepatitis model.

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