Plasma levels of luteinizing hormone and gonadal maturation of lambari females under different photoperiods

The objective of this work was to evaluate the effect of photoperiod on luteinizing hormone (LH) plasma levels and gonadal maturation of lambari females. One hundred and twenty adult lambaris, distributed into 12 aquaria of 20 L each, were randomly subjected to three different treatments, in a completely randomized design, and four replicates. Treatments were photoperiods in hours of light (L) and darkness (D): T1, 0 L:24 D; T2, 12 L:12 D; T3, 24 L:0 D. After 40 days, fish were subjected to fasting for 24 hours and, then, anesthetized. Immediately after slaughter, fish were weighed, and their gonads and livers were removed and weighed. Ovaries were weighed and immersed in Bouin's fixative solution for 24 hours and, then, kept in 70% alcohol until processing the material. Subsequently, the oocyte developmental stage was determined. No significant differences were observed between treatments for final weight, ovary weight, gonadosomatic index (GSI), hepatosomatic index (HSI) and LH levels. In all treatments, lambari females showed maturing ovaries with prevalence of vitellogenic oocytes. Photoperiod does not affect the LH levels and ovarian maturation in lambari females.

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Penggunaan serotonin dalam formulasi hormon pregnant mare serum gonadotropin dan antidopamin untuk menginduksi perkembangan gonad ikan ringau, Datnioides microlepis Bleeker, 1854 [Serotonin application in pregnant mare serum gonadotropin hormone and dopamin antagonist formulation to induce gonadal development of Indonesian tigerfish (Datnioides microlepis Bleeker, 1854)]

Jurnal Iktiologi Indonesia ◽

10.32491/jii.v17i1.302 ◽

2017 ◽

Vol 17 (1) ◽

pp. 29

Author(s):

NFN Bastiar ◽

Agus Oman Sudrajat ◽

Melta Rini Fahmi

Keyword(s):

Plasma Concentration ◽

Gonadosomatic Index ◽

Gonad Development ◽

Gonadal Development ◽

Hepatosomatic Index ◽

Small Fish ◽

Pregnant Mare Serum Gonadotropin ◽

Randomized Design ◽

Serum Gonadotropin ◽

Completely Randomized Design

The objective of study was to analyze the effect of serotonin (5-HT) in the formulation of hormones Pregnant Mare Serum Gonadotropin (PMSG) and dopamin antagonist (AD) hormones to gonad development of tigerfish. This study used completely randomized design (CRD) with five treatments of hormone namely: (P1) 1 ml of 0.9% NaCl (control); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG AD+10 mg+0.2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; and (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Each treatment was tested on five fish as individual replications. Hormone injected intramuscularly at the lower part of the dorsal fin of fish every 10 days. The fish were reared for 60 days. Fish that were used at this study were originate from natural catches as much as 25 fishes with 17.5-33.0 cm of total length and 118-926 g of body weight. During the study, fish fed using shrimp and small fish (live) twice daily at satiation. Measured parameters were gonadosomatic index (GSI), hepatosomatic index (HSI), 17P-estradiol (E2) plasma concentration and gonad maturity level based on morphology and histology examination. The results showed that the use of 2 mg of 5-HT are added to 20 IU PMSG and 10 mg AD (treatment P4) has stimulated the fish to had the highest GSI (2.38 ± 0.06%) and HSI (3,09±0,12%) which was significantly different to other treatment. The treatment (P4) could increase the E2 plasma concentration (37.14±2.99 pg.ml-1) two fold compared with the concentration before injection and stimulated the gonadal development to stage III. AbstrakPenelitian ini bertujuan untuk menganalisis pengaruh penggunaan serotonin (5- hydroxytryptamine atau 5-HT) dalam formulasi hormon Pregnant Mare Serum Gonadotropin (PMSG) dan antidopamin (AD) terhadap perkembangan gonad ikan ringau. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan (dosis.kg-1 bobot tubuh ikan): (P1) 1 ml NaCl 0,9% (kontrol); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG+10 mg AD+0,2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; dan (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Setiap perlakuan diujikan pada lima ekor ikan sebagai ulangan individu. Penyuntikan hormon dilakukan setiap 10 hari dengan lama penelitian 60 hari. Hormon disuntikkan secara intramuskular pada bagian bawah sirip punggung ikan uji. Ikan yang digunakan merupakan hasil tangkapan alam dengan ukuran panjang total 17,5-33,0 cm dan bobot tubuh 118-926 g. Selama penelitian, ikan uji diberi pakan berupa udang dan ikan-ikan kecil (hidup) dua kali sehari secara satiasi. Parameter yang diamati adalah indeks kematangan gonad, indeks hepatosomatik, konsentrasi estradiol-17p plasma dan tingkat kematangan gonad berdasarkan morfologi dan histologi gonad. Hasil penelitian menunjukkan penggunaan 2 mg 5-HT dalam 20 IU PMSG dan 10 mg AD (perlakuan P4) menghasilkan perkembangan gonad yang lebih baik dibandingkan perlakuan lainnya. Nilai indeks kematangan gonad, indeks hepatosomatik, dan konsentrasi E2 plasma tertinggi diperoleh pada perlakuan P4 dengan nilai masing-masing sebesar 2,38±0,06%; 3,09±0,12% dan 37,14±2,99 pg.ml-1. Nilai tersebut berbeda nyata p<0.05) dengan perlakuan lainnya. Perlakuan P4 meningkatkan konsentrasi E2 plasma dua kali lebih besar dibanding-kan sebelum penyuntikan serta menghasilkan perkembangan gonad yang mencapai tingkat kematangan gonad tahap III.

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Pengaruh Hormon Pregnan Mare Serum (PMSG) Murni dan Kombinasi terhadap Gonadosomatik Indeks, Hepatosomatik Indeks Ikan Bawal Bintang (Trachinotus blochii)

Journal of Aquaculture Science ◽

10.31093/joas.v2i1.14 ◽

2017 ◽

Vol 2 (1) ◽

Author(s):

Wiwin Kusuma Atmaja Putra ◽

Tengku Said Razai

Keyword(s):

Body Weight ◽

Gonadosomatic Index ◽

Hormone Treatment ◽

Hepatosomatic Index ◽

Pregnant Mare Serum Gonadotropin ◽

Randomized Design ◽

Absolute Growth ◽

Serum Gonadotropin ◽

Completely Randomized Design ◽

Trachinotus Blochii

The research was title The Effect of Pregnant Mare Serum Gonadotropin (PMSG) Pure and Combinations against Gonadosomatik Index, Hepatosomatic Index Silver Pompano (Trachinotus blochii). The purpose of this research was to obtain the best hormone treatment to increase the gonadosomatic index and Hepatosomatic Index. This research used a Completely Randomized Design with 3 treatments and 3 replicates of fish. The treatments include Control (0.5 ml NaCl), PMSG (20 IU kg-1 fish body weight) and PG600 (20 IU. Kg-1 fish body weight). Parameters calculated include Gonadosomatic Index (GSI), Hepatosomatic Index (HSI) and Absolute Growth. The best results were PG600 treatment with Gonadosomatic Index (0.12±0.06), Hepatosomatic Index (2.29±0.15 %) and Absolute Growth (85±31.32 g) for 4 weeks. The conclusion of this study is the provision of pure PMSG and combination with a dose of 20 IU. Kg-1 body weight can increase gonadosomatik index, hepatosomatic index and absolute growth of Silver Pompano fish. Keywords : Silver Pompano Fish, PMSG, PG600, Gonadosomatic Index

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Hormonal induction maturation of silver pompano Trachinotus blochii

Jurnal Akuakultur Indonesia ◽

10.19027/jai.19.1.61-73 ◽

2020 ◽

Vol 19 (1) ◽

pp. 61-73

Author(s):

Wiwin Kusuma Atmaja Putra ◽

Tengku Said Raza’i

Keyword(s):

Body Weight ◽

Gonadosomatic Index ◽

Hormone Treatment ◽

Hepatosomatic Index ◽

Gonad Maturation ◽

Maturity Level ◽

Randomized Design ◽

Absolute Growth ◽

Completely Randomized Design ◽

Trachinotus Blochii

ABSTRACT This study was aimed to acquire the most suitable hormone treatment to induce gonad maturation of silver pompano Trachinotus blochii. This study used a completely randomized design with four treatments and three replications, i.e NaCl 90% (control) (0.5 mL/kg), hCG (20 IU/kg fish body weight), PMSG (20 IU/kg fish body weight), and PG600 (20 IU/kg fish body weight). Parameters measured were gonadosomatic index (GSI), hepatosomatic index (HSI), gonad maturity level (TKG), gonad histology, and absolute growth. The best result was PG600 treatment with 0.12% gonadosomatic index, 2.29% hepatosomatic index, TKG IV, and 85 g absolute growth for four weeks. This study concludes that the most suitable hormone to induce gonad maturation of silver pompano Trachinotus blochii is PG600 with 20 IU/kg body weight, which effectively affects GSI, HSI, and absolute growth of silver pompano. Keywords: hCG, maturation, PG600, PMSG, silver pompano. ABSTRAK Penelitian ini bertujuan untuk mendapatkan hormon yang terbaik untuk menginduksi maturasi gonadikan bawal bintang. Hormon yang digunakan pada penelitian ini adalahkontrol NaCl 90% (dosis 0,5 mL/kg), hCG(dosis 20 IU/kg bobot tubuh ikan), PMSG (dosis 20 IU/kg bobot tubuh ikan), dan PG600 (dosis 20 IU/kg bobot tubuh ikan). Parameter yang diamati adalah indeks gonadosomatik (GSI), indeks hepatosomatik (HSI), tingkat kematangan gonad (TKG), histologi gonad, dan pertumbuhan bobot mutlak. Hasil terbaik adalah perlakuan PG600 dengan gonadosomatik indeks (0,12%), hepatosomatik indeks (2,29%), TKG IV,dan pertumbuhan mutlak (85 g) selama empat minggu. Kesimpulan penelitian ini adalah perlakuan hormone yang berpotensi menginduksi maturasi gonad ikan bawal bintang adalah hormon PG600 dengan dosis 20 IU/kg bobot tubuh dikarenakan adanya pengaruh nyata tehadap parameter GSI, HSI dan pertumbuhan mutlak ikan bawal bintang. Kata kunci: ikan bawal bintang, hCG, PMSG, PG600, maturasi.

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PENGGUNAAN SLUDGE SEBAGAI PAKAN CALON INDUK IKAN NILA MERAH (Oreochromis sp.)

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.9033 ◽

2003 ◽

Vol 5 (2) ◽

pp. 11

Author(s):

Rustidja Rustidja

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth ◽

Control Group ◽

Fish Feed ◽

Commercial Fish ◽

Gonadal Maturation ◽

Randomized Design ◽

Completely Randomized Design ◽

Maturation Index

The aim of study was to know the effect of sludge in the ration on the gonadal growth of Oreochromis sp. broodstock. Experimental design used in this experiment was completely Randomized Design. The broodstocks were fed with the ration containing 0-40% of sludge, or with a commercial fish feed as a control.The results were not significantly differences between the treatment in all parameters. The sludge in ration ranging from 0 to 40% resulted the level of gonadal maturation of 4, 6, 7, dan 9. The value of Gonadal Maturation Index on 0,96 to 3,98 % and the control group on 1,75 %. The specific growth rate of broodstock fed with the ration ranged from 1,1 to 1,62 Bw/day and that of the development of gonadal control group was 1,97. The survival rate were variated between 14,29 to 100% and that of the development of gonadal control group is 71,43%. The ration containing 40% sludge is the most suitable feed for maturation in Oreochromis.

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Reproduction of the catfish Iheringichthys labrosus (Lütken) (Pisces, Siluriformes) in Furnas reservoir, Minas Gerais, Brazil

Revista Brasileira de Zoologia ◽

10.1590/s0101-81752004000200006 ◽

2004 ◽

Vol 21 (2) ◽

pp. 193-200 ◽

Cited By ~ 19

Author(s):

José Enemir dos Santos ◽

Nilo Bazzoli ◽

Elizete Rizzo ◽

Gilmar Bastos Santos

Keyword(s):

Feeding Activity ◽

Gonadosomatic Index ◽

Secretory Activity ◽

Minas Gerais ◽

Reproductive Period ◽

Mature Stage ◽

Hepatosomatic Index ◽

Gonadal Maturation ◽

Caudal Region ◽

Iheringichthys Labrosus

The reproductive biology of Iheringichthys labrosus (Lütken, 1874) was studied in Furnas reservoir, Minas Gerais, Brazil. The fishes, 323 males and 817 females, were captured bimonthly from August 1993 to July 1994 by using gill nets with 3-10 cm mesh size. Gonadal maturation of the specimens was analyzed through macro and microscopical characteristics of the gonads. The secretory activity of the tubules of the caudal region of the testis was analyzed during the maturational testicular cycle. Females and males were in reproductive activity throughout the year in the reservoir. The peak of advanced maturation/mature stages occurred from October to January and of spawned/spent from February to May. The spawning of I. labrosus was identified as fractional or multiple type: occurring in the reservoir or in tributaries around it. The oocyte development of the species was asynchronous as different clusters of oocytes occurred simultaneously in the ovarian parenchyma. The epithelium of the tubules of the caudal region of the testis was in high secretory activity during advanced maturation/mature stage and this activity was absence in resting. The gonadosomatic index followed gonadal maturation, and the hepatosomatic index of females was lowest in advanced maturation/mature probably due the contribution of the liver in the vitellogenesis. The variations of the stomach repletion index, coelomic fat and condition factor indicated that the feeding activity was reduced and that the energetic reserves were consumed during the reproductive period.

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Use of different combinations of enzyme complexes in broiler diets

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n5p2907 ◽

2021 ◽

Vol 42 (5) ◽

pp. 2903-2924

Author(s):

Lindolfo Dorcino dos Santos Neto ◽

◽

Julyana Machado da Silva Martins ◽

Genilson Bezerra de Carvalho ◽

Roberto Moraes Jardim Filho ◽

...

Keyword(s):

Basal Diet ◽

Experimental Period ◽

Nutrient Digestibility ◽

Enzyme Complex ◽

Ether Extract ◽

Feed Conversion ◽

Randomized Design ◽

Final Weight ◽

Completely Randomized Design ◽

Enzyme Complexes

Two experiments were carried out to investigate the effect of “on top” addition of different enzyme complexes, the enzyme α-galactosidase and three sources of the enzyme phytase available on the market, in broiler diets. In the first experiment, 1260 one-day-old Cobb 500® chicks were distributed into seven treatments in a completely randomized design (CRD) with six replicates and 30 birds/replicate. Treatments consisted of combinations of different enzyme complexes, namely, complex A (phytase, protease, xylanase, ß-glucanase, cellulase, amylase, pectinase), complex B (protease and cellulase) and complex C (xylanase, amylase and protease); isolated α-galactosidase (GAL); and three sources of phytase (P1, P2 and P3) in the diet. The treatments were formulated as follows: T1 - basal diet (BD); T2 - BD + enzyme complex A + enzyme complex B (BDAB); T3 - BDAB + GAL; T4 - BD + complex A + GAL; T5 - BD + complex C + P1 + GAL (BDCG); T6 - BDCG + P2; and T7 - BDCG + P3. The following variables were measured in the experimental period of 42 days: feed intake (FI), weight gain (WG), average final weight (AFW), feed conversion (FC), and carcass yield. Significant differences occurred for AFW, WG and FC in the pre-starter phase. In the second experiment, 112 Cobb 500® chicks aged 25 days were distributed into seven treatments in a CRD with four replicates and four birds/replicate. Treatments were the same as in the first experiment. Nutrient digestibility was evaluated in an experimental period of seven days. Differences were found in the metabolism coefficient of ether extract (MCEE). Dietary inclusion of enzyme complexes improves the AFW and WG of chickens from 1 to 7 days of age and MCEE in the grower phase.

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Performance, hematology, and immunology of pacu in response to dietary supplementation with fructooligosaccharides

Pesquisa Agropecuária Brasileira ◽

10.1590/s1678-3921.pab2021.v56.02460 ◽

2021 ◽

Vol 56 ◽

Author(s):

Maday Priscila Piva Deon ◽

Álvaro José de Almeida Bicudo ◽

Ricardo Yuji Sado

Keyword(s):

Specific Growth ◽

Dietary Supplementation ◽

Mean Corpuscular Hemoglobin ◽

Feed Conversion ◽

Corpuscular Hemoglobin ◽

Immunological Parameters ◽

Fish Diets ◽

Randomized Design ◽

Final Weight ◽

Completely Randomized Design

Abstract The objective of this work was to evaluate the effects of dietary supplementation with fructooligosaccharides (FOS) on the performance, hematology, and immunology of pacu (Piaractus mesopotamicus) juveniles. A total of 0 (control), 2.0, and 4.0 g kg-1 of the probiotic were added to the fish diets. Fish (49.3±4.3 g) were allotted into 12 tanks of 60 L, in a completely randomized design (n=4). After 60 days, final weight, weight gain, specific growth rate, and feed conversion ratio were improved in the fish fed with the prebiotic, although feed intake was not affected by the treatments. The number of red blood cells was higher only in fish fed with 2.0 g kg-1 FOS. The opposite was observed for the hematimetric indices mean corpuscular volume and mean corpuscular hemoglobin, which decreased in fish fed with 2.0 g kg-1 FOS. The number of defense cells, such as leukocytes and thrombocytes, also increased in the prebiotic treatments. The evaluated immunological parameters were not influenced by prebiotic supplementation. Dietary FOS supplementation improved pacu growth and health.

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Purified glycerin in balanced diets for chicken broilers from 8 to 21 days old

Bioscience Journal ◽

10.14393/bj-v35n5a2019-39540 ◽

2019 ◽

Vol 35 (5) ◽

Author(s):

Mônica Calixto da Silva ◽

Roberta Gomes Marçal Vieira Vaz ◽

Kênia Ferreira Rodrigues ◽

Luciano Fernanades Sousa ◽

José Henrique Stringhini ◽

...

Keyword(s):

Weight Gain ◽

Feed Intake ◽

Control Diet ◽

Metabolizable Energy ◽

Feed Conversion ◽

Gross Margin ◽

Randomized Design ◽

Final Weight ◽

Apparent Metabolizable Energy ◽

Completely Randomized Design

This paper aimed to evaluate the metabolizability, performance and economic viability of purified glycerin inclusion in balanced diets fed to chicken broilers from 8 to 21 days old. Two experiments were conducted. In the first experiment, 100 broilers (14 days old) were distributed in a completely randomized design into two treatments, with five replications of 10 broilers. Treatments consisted of a control diet and a test diet, in which purified glycerin replaced 10% of the control diet. In the second experiment, 200 broilers (8 days old) were distributed in a completely randomized design into four treatments (0, 2, 4 and 6% of purified glycerin inclusion), with five replications of 10 broilers. The weight gain, feed intake, feed conversion, final weight, apparent metabolizable energy (AME), nitrogen-corrected apparent metabolizable energy (AMEn), metabolizability coefficients of dry matter (DMMC), crude protein (CPMC) and gross energy (GEMC), and the cost of feed per kg of broiler produced were evaluated. The AME, AMEn, DMMC, CPMC and GEMC from the purified glycerin were 3790 and 3560 kcal/kg, and 83.72, 71.52 and 86.27%, respectively. The glycerin levels did not affect (p>0.05) any of the performance characteristics (weight gain, feed intake, feed conversion and final weight). The lowest feeding cost and the highest gross margin were obtained for broilers fed with 6% purified glycerin. The inclusion of 6% purified glycerin in balanced diets for broilers from 8 to 21 days old was technically and economically feasible.

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Use of golden mussel and wattle tannin in the supply of cut chickens

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n5p1951 ◽

2019 ◽

Vol 40 (5) ◽

pp. 1951

Author(s):

Douglas Fernando Bayerle ◽

Ricardo Vianna Nunes ◽

Lucas Wachholz ◽

Taciana Maria de Oliveira Bruxel ◽

José Geraldo de Vargas Junior ◽

...

Keyword(s):

Weight Gain ◽

Toxic Metal ◽

Experimental Unit ◽

Feed Conversion ◽

Liver Size ◽

Randomized Design ◽

Final Weight ◽

Completely Randomized Design ◽

Golden Mussel ◽

Different Levels

This study aimed to evaluate the use of acacia tannin as a toxic metal adsorbent in the diets of broilers fed with the inclusion of golden mussel meal in substitution for calcitic limestone. In the first trial, 648 male 21-day-old animals were divided among six treatments and six replicates, with 18 birds per experimental unit, organized in a completely randomized design (DIC). The treatments were diets with different acacia tannin levels (0, 250, 500, 750, 1000, or 1250 g ton-1). The results indicated that 250 g ton-1 of tannin was not harmful to weight gain, final weight, or feed conversion. Tannin levels caused a decrease in carcass yield and fat deposition and an increase in liver size. For the second trial, 900 male broilers of 21 days of age were used and distributed in a DIC, with different levels of substitution of calcitic limestone by the golden mussel meal (0, 25, 50, 75, or 100 %) and supplementation or not with acacia tannins (250 g ton-1), with 5 replicates and each experimental unit being composed of 18 birds. The performance data show that the use of 250 g ton-1 of tannin is detrimental to bone performance and resistance and golden mussel meal can be used to substitute up to 100 % of the limestone in the diets without affecting the variables studied.

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A by-product of pinto beans in diets for quail in the laying phase

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v42i1.48883 ◽

2020 ◽

Vol 42 ◽

pp. e48883

Author(s):

Gisele Maria Nunes Vieira ◽

Adriana Aparecida Pereira ◽

Dirceu Neutzling Griep Júnior ◽

Wilson Araújo da Silva ◽

Samila Santos Viana ◽

...

Keyword(s):

Japanese Quail ◽

Egg Quality ◽

Quality Parameters ◽

Egg Laying ◽

Egg Mass ◽

Feed Conversion ◽

Randomized Design ◽

Final Weight ◽

Completely Randomized Design ◽

Pinto Beans

The aim of this study was to evaluate levels of inclusion of pinto beans in diets for quail in the laying phase on their performance and egg quality. A total of 280 Japanese quail in the laying phase were distributed into five treatments in a completely randomized design with eight replicates and seven birds per plot. Treatments consisted of five levels (0, 2, 4, 6, and 8%) of inclusion of pinto beans in the diets, which remained isoenergetic and isoproteic. The experiment began with the quail at 70 days of age and lasted 63 days, with their performance assessed every 21 days and egg-quality parameters on the last two days of each cycle. The following parameters were evaluated: final weight, feed intake, egg-laying rate, feed conversion (per dozen eggs and per egg mass), egg weight, eggshell thickness, specific gravity, yolk color, and weights of shell, yolk, and albumen. No difference was detected between the bean inclusion levels for any of the performance and egg-quality traits assessed or the birds' final weight. In conclusion, up to 8% raw pinto beans may be added in diets for Japanese quail in the laying phase without compromising their performance or internal-external egg quality.

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