Isolation and screening of the endophytes possessing antioxidant activity from Rutaceae and Zingiberaceae plants

Zingiberaceae and Rutaceae have been many applications in the treatment of diseases. Their leaves, stems, rhizomes and fruits… containing essential oils which could offer useful habitats for different endophyte groups. In this research, the strains of endophyte Streptomyces sp., Aspergillus terreus, Cladosporium sp., Penicillium sp. were isolated from different plant parts of galangal, ginger, laurel and their antioxidant activities were determined. The antioxidant activity by using DPPH methos applied on these strain was with the following results: R-TN3 strain showed the highest antioxidant activity (62.32 %), followed by G1- TL3 and NQ-TN3 and the T-TG3 was the lowest (30.95 %).

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Evaluation of antioxidant and antimicrobial activity of Ziziphora ‎clinopodiodes essential oil eluted at different times

Current Bioactive Compounds ◽

10.2174/1573407216999200507122039 ◽

2020 ◽

Vol 16 ◽

Author(s):

Mojgan Alizadeh ◽

Akram Arianfar ◽

Ameneh Mohammadi

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Positive Control ◽

Essential Oil Composition ◽

Oil Composition ◽

Citronellic Acid ◽

Antimicrobial And Antioxidant Activity

Objective: Ziziphora clinopodioides is an edible medicinal plant belongs to the Labiatae family that widespread all over Iran. It used as culinary and also in cold and cough treatments in Iran. The aim of present work was to evaluate the effect of different timeframes during the hydrodistillation on essential oil composition, antimicrobial and antioxidant activity. Materials and Methods: The essential oil of Z. clinopodiodes was extracted via hydrodistillation with Clevenger apparatus. The fractions of essential oil were captured at 6 times from the beginning of the distillation: (10, 20, 60, 120, 180 and 240 min). The fractions of essential oil were analyzed by GC/MS and their antibacterial, antifungal and antioxidant activities were studied by Disk - well diffusion and DPPH methods respectively. Results: Six distillation times and whole essential oil were captured during the hydrodistillation. Essential oil yield dropped off significantly during distillation progressed (1.0% for 10 min and 0.025 for 240 min). 1,8 Cineol, Isomenthone, Pulegone, Piperitenone and Citronellic acid were major compounds in fractions and they were affected by distillation times. Pulegone was major compound in all of essential oils. In antioxidant activity assay, whole essential oil was stronger than was stronger than positive control and fractions of essential oil, because of higher levels of Isomenthone, Piperitenone and Citronellic acid. Strongest antimicrobial activity against S. aureus, E. coli and C. albicans was observed from 10 min fraction. Conclusion: Our results indicated that distillation time can create essential oils with specific properties and we can achieve to more efficient essential oil in short times.

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Profiling of Essential Oils Components and Polyphenols for Their Antioxidant Activity of Medicinal and Aromatic Plants Grown in Different Environmental Conditions

Agronomy ◽

10.3390/agronomy10050727 ◽

2020 ◽

Vol 10 (5) ◽

pp. 727 ◽

Cited By ~ 2

Author(s):

Antonios Chrysargyris ◽

Maria Mikallou ◽

Spyridon Petropoulos ◽

Nikolaos Tzortzakis

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oils ◽

Environmental Conditions ◽

Antioxidant Activities ◽

Added Value ◽

Total Phenols ◽

Aromatic Plants ◽

Medicinal And Aromatic Plants ◽

Antioxidant Power

In the present study, the yield, the chemical composition, and the antioxidant activities of the essential oils (EOs) of eight medicinal and aromatic plants (MAPs) cultivated under two environmental conditions characterized by a different altitude (namely mountainous and plain) were evaluated. Cultivation at different environmental conditions resulted in significant differences in the chemical composition and antioxidant activity for most of the studied species. In particular, high altitudes resulted in increased phenolic compounds’ content and antioxidant activity for artemisia plants, while specific parameters increased in the case of spearmint (total phenols) and rosemary (flavonoids). In contrast, in pelargonium, all the tested parameters were positively affected in the plain area, whereas, for laurel and sage, only flavanols remained unaffected. EO yield in mountainous pelargonium and spearmint decreased while, in mountainous laurel, pelargonium and spearmint increased when compared to plain areas. In addition, the major EO constituents’ content for most of the species were affected by environmental conditions. The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and ferric reducing antioxidant power (FRAP) were variably correlated with total phenols, flavonoids, and flavanols, depending on the species and the altitude. Lastly, in limited cases, antioxidant activity (DPPH or FRAP values) was positively correlated with some EO components (e.g., borneol and β-pinene in artemisia and laurel plants grown in the plain, respectively, or 1,8-cineole in mountainous grown verbena plants). In conclusion, environmental conditions (altitude) affected antioxidants’ content and EO yield and composition of the studied MAPs. These findings can be used to introduce cultivation of MAPs in specific ecosystems for the production of high added value products.

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Aroma Profile and Antioxidant Activity of Essential Oil from Alpinia zerumbet

Natural Product Communications ◽

10.1177/1934578x1701200842 ◽

2017 ◽

Vol 12 (8) ◽

pp. 1934578X1701200 ◽

Cited By ~ 1

Author(s):

Eisuke Kuraya ◽

Rui Yamashiro ◽

Akiko Touyama ◽

Shina Nakada ◽

Kenta Watanabe ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Essential Oils ◽

Volatile Compound ◽

Antioxidant Activities ◽

Principal Component ◽

Inverse Correlation ◽

Gas Chromatography Mass Spectrometry ◽

Individual Plant ◽

Future Production

The essential oil of A. zerumbet, obtained from the leaves through steam distillation, has a distinct aroma and shows antioxidant activity. In this study, we evaluated the yields, antioxidant activity, and volatile compound composition of essential oils extracted from the leaves of A. zerumbet to better understand how these characteristics may vary among individual plants, and how they may differ with location of growth and season in which leaves are harvested. Gas chromatography–mass spectrometry (GC/MS) and principal component analysis (PCA) revealed that chemical composition in each individual plant was not affected by harvest season or growth area. Furthermore, GC/MS and antioxidant activity assays revealed that although the antioxidant activity and yield of these essential oils differed significantly between individual plants and collecting seasons, there was a strong inverse correlation between the yields of essential oils and their antioxidant activity. The yield, the major volatile compound compositions, the enantiomeric ratio of the few monoterpenes, and the antioxidant activities of A. zerumbet essential oil varied significantly among individual plants, and probably reflected the genetic variability within the species. This suggests the plausibility of selecting lineages of A. zerumbet to optimize future production of valuable essential oils.

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Phytochemical Analysis and Assessment of Biological Properties of Essential Oils Obtained from Thyme and Rosmarinus Species

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666191019124630 ◽

2020 ◽

Vol 21 (5) ◽

pp. 414-424

Author(s):

Amira Zaïri ◽

Sahar Nouir ◽

Mohamed A. Khalifa ◽

Bouraoui Ouni ◽

Houda Haddad ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oils ◽

Traditional Medicine ◽

Plant Species ◽

Antioxidant Activities ◽

Biological Properties ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Analysis ◽

Cytotoxic Potential ◽

Thymus Algeriensis

Background: The plant species Thymus algeriensis (TA); Thymus capitatus (TC) and Rosmarinus officinalis (RO), are widely used in traditional medicine in Tunisia. The bioactivities of their essential oils have been also reported previously. The main objective of this work was to assess the phytochemical composition, the antioxidant activity, cytotoxic potential and the antibacterial, antifungal, of the essential oil (EO) of these plants. Methods: Gas chromatography–mass spectrometry (GC-MS) was used to identify and quantify the constituents of the tested EO, chemical tests and spectrophotometric methods were used for antioxidant activities and for the screening, the quantification of phytochemicals. The cytotoxic potential of the EO was checked using HCT 116 cultures. Extracts were evaluated for their antibacterial potential by the microdilution method. Antifungal activities were tested using the Poisoned food method against: Aspergillus niger and Aspergillus flavus. Results: The EO of tested plants presented several components, mainly monoterpenes and sesquiterpenes. Results revealed that T. capitatus EO is not toxic comparing to the other tested samples. Phenolic compounds were detected and this EO showed excellent antioxidant activity presenting dose dependent relationship. Regarding antimicrobial activity, T. capitatus EO, also had the highest inhibition against all tested bacteria and fungi. Conclusions: This study showed the importance of the bioactivities (antioxidant, antimicrobial, and safety potential) of EOs of the plant species TC, RO, and TA used in traditional medicine.

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Evaluation of antibacterial and antioxidant activities of three types of benzoin resin

European Journal of Chemistry ◽

10.5155/eurjchem.9.4.408-411.1755 ◽

2018 ◽

Vol 9 (4) ◽

pp. 408-411

Author(s):

Zineb Hacini ◽

Fatima Khedja ◽

Ibrahim Habib ◽

Zaouia Kendour ◽

Zineb Debba

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antioxidant Activities ◽

Ic50 Values ◽

Therapeutic Properties ◽

Resin Extract ◽

And Staphylococcus Aureus

The benzoin resin is used extensively in traditional medicine for its many reported therapeutic properties. The essential oils of three different types of benzoin resin were extracted using the traditional method in this study. The yield of essential oils of the white, red and gray types of resin was 1.01, 0.92 and 0.54%, respectively. The obtained extracts were tested against two types of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. The tests showed that essential oil of gray type resin is effective against both Escherichia coli (14 mm) and Staphylococcus aureus (11 mm). The antioxidant activity has been also evaluated to compare the efficiency of different type of resin with DPPH· assay. In the DPPH· system, the antioxidant activity of the red resin extract (0.01 μg/mL) was superior to that of the white (27.32 μg/mL) and gray (42.90 μg/mL) extracts, with IC50 values, respectively.

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Chemical Compositions and Antioxidant Activities of Indonesian Citrus Essential Oils and Their Elucidation Using Principal Component Analysis

10.20944/preprints202011.0586.v1 ◽

2020 ◽

Author(s):

Euis Julaeha ◽

Kristin Shinta Dewi ◽

Mohamad Nurzaman ◽

Tatang Wahyudi ◽

Tati Herlina ◽

...

Keyword(s):

Principal Component Analysis ◽

Antioxidant Activity ◽

Essential Oils ◽

Antioxidant Activities ◽

Principal Component ◽

Component Analysis ◽

Chemical Compositions ◽

Dpph Method ◽

Ic50 Values ◽

Citrus Essential Oils

Citrus essential oils (EOs) have various bioactivities like antioxidants, with many applications. Antioxidant activities depend on the chemical compositions of the EOs, which are affected by climate, soil, and geographical region. Thus, investigations on chemical compositions and antioxidant activities of Citrus EOs in different countries are valuable. In this study, we distilled EOs from peels of Indonesian-grown Citrus, including C. nobilis, C. limon, C. aurantifolia, C. amblycarpa, and Citrus spp.Chemical compositions of EOs were analyzed using Gas Chromatography-Mass Spectrometer (GC-MS), whereas the antioxidant activities were determined by employing 2,2-diphenyl-2-picrylhydrazyl (DPPH) method. Furthermore, principal component analysis (PCA) was applied to elucidate the main contributing compounds for antioxidant activity. The results show that all EOs possess unique chemical characteristics, with limonene as the majority constituent. For antioxidant activities, C. limon and C. amblycarpa EOs are the two strongest, IC50 values below 7.00 μL/mL. PCA approach suggests that -terpinene mainly contributes to the high antioxidant activities of C. limon and C. amblycarpa. Moreover, o-cymene, thymol, p-cymene, and α-pharnesene may also be responsible for the antioxidant activity of C. limon EO. These results are valuable information for the applications of Citrus EOs as antioxidant sources.

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Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220952 ◽

2021 ◽

Vol 22 (9) ◽

Author(s):

THEERAPHAN CHUMROENPHAT ◽

Surapon Saensouk ◽

PIYAPORN SAENSOUK

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Antioxidant Activities ◽

Dry Weight ◽

Total Phenolic ◽

Antioxidant Power ◽

Plant Parts ◽

Ferric Reducing Antioxidant Power ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

Abstract. Chumroenphat T, Saensouk S, Saensouk P. 2021. Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand. Biodiversitas 22: 4036-4044. Genus Cornukaempferia (Zingiberaceae) were used as medicinal plants in Thailand. Cornukaempferia has never been reported the chemical compounds and antioxidant activity before. This study was designed to identify the chemical composition and antioxidant activities in root, rhizome, stem, and leaves of Cornukaempferia aurantiflora, C. larsenii, and C. longipetiolata. DPPH scavenging activity and Ferric reducing antioxidant power (FRAP) assays assessed the chemical composition and antioxidant activity. The results showed that total phenolic content (TPC) and total flavonoid content (TFC) found different plant parts obtained in this study were in the order of root>rhizomes>leaves>?stems. The C. aurantiflora had a high TPC of 416.39 mg GAE/g dry weight, while individual phenolics, such as vanillic acid at 256.90 mg/g dry weight, showed high contents in the leaf part. For C. larsenii, TFC and individual flavonoids, such as catechin, have high 62.52 and 56.47 mg/100g dry weight values in the roots, respectively. The antioxidant activities in different species of different plant parts (DPPH: root>leaves?> rhizomes?>?stems; FRAP: leaves?>root>?rhizomes?>?stems). The genus Cornukaempferia is a rich source of bioactive compounds and antioxidant activity. Therefore, the genus Cornukaempferia will be used as medicine and food to produce products with health benefits in the future.

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Acetylcholinesterase Inhibition and Antioxidant Activity of Essential Oils from Schinus Areira L. and Schinus Longifolia (Lindl.) Speg

Natural Product Communications ◽

10.1177/1934578x0900400628 ◽

2009 ◽

Vol 4 (6) ◽

pp. 1934578X0900400 ◽

Cited By ~ 1

Author(s):

Ana P. Murray ◽

María S. Vela Gurovic ◽

Silvana A. Rodriguez ◽

María G. Murray ◽

Adriana A. Ferrero

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Acetylcholinesterase Inhibition ◽

Radical Scavenger ◽

Radical Scavenger Activity ◽

Scavenger Activity

The essential oils of Schinus areira L. and S. longifolia (Lindl.) Speg. (Anacardiaceae) have been studied for their in vitro anti-acetylcholinesterase and antioxidant activities. The chemical composition of the oils obtained by hydrodistillation was determined by GC-MS. Fruit and leaf oils of S. areira were analyzed separately. The essential oil from S. longifolia elicited marked enzymatic inhibition (IC50 = 20.0 ± 1.0 μg/mL) and showed radical scavenger activity (IC50 = 25.2 ± 2.4 μg/mL). The essential oil from S. areira leaves was more active than that of the fruits in both bioassays.

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