scholarly journals Effect of Crocin on Bax, Bcl-2, and Oxidative Stress Markers in the Testis Tissue of Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 25 (4) ◽  
pp. 340-351
Author(s):  
Ghazal Ataei ◽  
◽  
Raheleh Rahbarian ◽  
Majid Rajabian Noghondar ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Herbal Medicines ◽  
B Cell Lymphoma ◽  
Treated Group ◽  
Diabetic Rats ◽  
Control Group ◽  
Stress Markers ◽  
Significant Difference ◽  
Anti Oxidant ◽  
Testis Tissue

Aims Type 1 diabetes is a high-prevalent endocrine disease and causes oxidative stress in the testis tissue. In the treatment of diabetes, the tendency toward herbal medicines use is increasing. This study aimed to investigate the crocin effect on the Bax, Bcl2 (B-cell lymphoma 2), and anti-oxidant levels of streptozotocin-induced diabetic rats. Methods & Materials This experimental study was performed at Payam Noor University. In total, 24 rats were divided into 4 groups, as follows: control, untreated diabetic, and 2 crocin-treated (50, 100 mg/mL, 25 days intraperitoneal injection) diabetic groups. The diabetic groups were diabetic rats receiving the Intraperitoneal (IP) injection of Streptozotocin (STZ). On day 25, the testicles were dissected to evaluate antioxidant enzymes, Bax and Bcl2. The obtained results were analyzed in SPSS using one-way Analysis of Variance (ANOVA) and Least Significant Difference (LSD) test. Findings The pro-apoptotic Bax and malondialdehyde levels in the treated group with a concentration of 100 mg/mL of crocin was significantly reduced, compared to the treated group with a concentration of 50 mg/mL of crocin and the control group. However, the level of pro-apoptotic Bcl-2 and glutathione peroxidase, superoxide dismutase, and catalase enzymes in the treated group with a concentration of 100 mg/mL of crocin significantly increased, compared to the treated group with a concentration of 50 mg/mL of crocin, and the control group (P<0.05). Such finding indicates the effect of crocin concentration. Additionally, crosin significantly reduced the glucose level in diabetic rats. Conclusion Crocin improved the antioxidant indicators and diabetes-induced damages in the testis tissue of diabetic rats.

scholarly journals The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1332
Author(s):  
Gilda M. Iova ◽  
Horia Calniceanu ◽  
Adelina Popa ◽  
Camelia A. Szuhanek ◽  
Olivia Marcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Rats ◽  
Gingival Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Significant Difference ◽  
The Impact ◽  
Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

scholarly journals Evaluation of Oxidative Stress in Patients with Fracture Admitted to Khatam Al-Anbia Hospital in Zahedan

2020 ◽  
Vol 18 (3) ◽  
Author(s):  
Mansour Karajibani ◽  
Farzaneh Montazerifar ◽  
Faezeh Kazemi ◽  
Ali Reza Dashipour
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activities ◽  
Control Group ◽  
Case Group ◽  
Cross Sectional ◽  
Stress Markers ◽  
Significant Difference ◽  
Before And After ◽  
Markers Of Oxidative Stress ◽  
The Mean

Background: Caused by an imbalance in the body’s oxidant and antioxidant status, oxidative stress can give rise to tissue damage and exacerbation of many diseases. Objectives: This study investigated the oxidative stress markers in patients with fractures and healthy subjects. Methods: In a cross-sectional case-control study, 40 patients with fractures admitted to an orthopedic ward and 40 healthy, non-fractured patients were selected using convenience sampling. Serum was analyzed for oxidant and antioxidant activities based on standard methods. P < 0.05 was considered statistically significant. Results: There was a significant difference in mean TAC between the case (748.2 ± 302.83 μmol/L) and control (984.90 ± 207.02 μmol/L) groups (P = 0.003). The mean MDA was 16.61 ± 4.16 µmol/L in the case group and 18.45 ± 5.43 µmol/L in the control group (P = 0.09). The mean SOD was 63.41 ± 16.67 U/g Hb in the case group and 58.54 ± 21.83 U/g Hb in the control group (P = 0.2). There was no significant difference between the two groups in terms of BMI and other variables. Conclusions: A significant difference existed in TAC between the two groups, which indicated increased oxidative stress in patients. However, oxidative stress could occur before and after undergoing fractures. The results suggested a slight, but not significant, difference between the two groups in the SOD and MDA scores. More conclusive results are required to determine the enzymatic and non-enzymatic markers of oxidative stress in larger sample sizes and during different stages of treatment.

scholarly journals ANTIHYPERGLYCEMIC AND ANTIOXIDATIVE EFFECTS OF LYGODIUM MICROPHYLLUM IN ALLOXAN INDUCED DIABETIC RATS

2018 ◽  
Vol 10 (12) ◽  
pp. 75
Author(s):  
D. G. Syahidah Nadiah Binti Abdull Majid ◽  
Mohammad Iqbal
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Antioxidative Effects ◽  
And Oxidative Stress

Objective: The antihyperglycemic and antioxidative effects of L. microphyllum were evaluated by using in vivo methods in normal and alloxan induced diabetic rats.Methods: Diabetes was induced in Sprague Dawley rats by injecting alloxan through intravenous (i. v) at a dose of 100 mg/kg of body weight. Aqueous extract of L. microphyllum at different doses (400, 200 and 100 mg/kg of body weight) was administered orally (orogastric intubation) for 14 d. Blood glucose and oxidative stress markers were measured. Hematoxylin and eosin staining method were used to examine the pancreatic tissues.Results: At the 14 d interval, fasting blood glucose showed a reduction in serum glucose levels in animals pretreated with L. microphyllum compared with alloxan alone treated group. Oxidative stress was noticed in rat’s pancreatic tissue as evidenced by a significant decrease in glutathione level, glutathione reductase, glutathione-S-transferase, and catalase activities. Malondialdehyde showed a significant increase compared to the normal saline-treated control group. Serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with L. microphyllum at a dose level of 100, 200 and 400 mg/kg body weight leaves extract for 14 d significantly prevented these alterations and attenuated alloxan-induced oxidative stress (P<0.05).Conclusion: The results of the present study indicated that the antihyperglycemic potential of L. microphyllum might be ascribable to its antioxidant and free radical scavenging properties. Thus, it is concluded that L. microphyllum may be helpful in the prevention of diabetic complications associated with oxidative stress.

scholarly journals Investigation of hallmarks of carbonyl stress and formation of end products in feline chronic kidney disease as markers of uraemic toxins

2018 ◽  
Vol 21 (6) ◽  
pp. 465-474 ◽  
Author(s):  
Emanuela Valle ◽  
Liviana Prola ◽  
Diana Vergnano ◽  
Roberta Borghi ◽  
Fiammetta Monacelli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Biochemical Parameters ◽  
Advanced Oxidation ◽  
Treated Group ◽  
Control Group ◽  
Advanced Oxidation Protein Products ◽  
Significant Difference ◽  
End Products

Objectives Cats are commonly affected by chronic kidney disease (CKD). Many reactive carbonyl intermediates and end products originating from the oxidative stress pathways are recognised as uraemic toxins and may play a role in CKD progression. The aim of the present study is to confirm whether carbonyl end-product formation is higher in cats affected by CKD and to assess whether an angiotensin-converting enzyme inhibitor (ACEi) might affect these hallmarks. Methods Twenty-two cats were divided into three groups: a control group (CG), cats with CKD and cats with CKD treated with an ACEi. Serum levels of pentosidine, carboxymethyllysine, advanced oxidation protein products, malondialdehyde, methylglyoxal and hexanoyl-lysine were measured. In addition, biochemical parameters and systolic blood pressure were evaluated. After checking for normality, comparisons between groups were performed followed by multiple comparison tests. P values ⩽0.05 were considered significant. Correlations between concentrations of the considered biomarkers and of the other metabolic parameters were investigated. Results Advanced oxidation protein products, malondialdehyde and hexanoyl-lysine concentrations were significantly higher in CKD and ACEi-treated groups compared with the CG ( P <0.05). Carboxymethyllysine increased in the ACEi-treated group when compared with the CG, whereas intermediate values of these biomarkers were found in the CKD group ( P <0.05). The ACEi-treated group showed the highest values of carboxymethyllysine, advanced oxidation protein products and hexanoyl-lysine. By contrast, the CKD group had the highest concentration of malondialdehyde. No statistically significant difference was found in the levels of pentosidine or methylglyoxal. End products correlated with creatinine and urea and with each other. Conclusions and relevance Significantly high concentrations of both intermediate and end products of carbonyl/oxidative stress were detected in CKD cats. This is the first study to have concurrently taken into account several uraemic toxins and biochemical parameters in cats affected by CKD.

scholarly journals Effect ofAgaricus blazeiMurill on the Pulmonary Tissue of Animals with Streptozotocin-Induced Diabetes

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Fábio Cangeri Di Naso ◽  
Rodrigo Noronha de Mello ◽  
Sílvia Bona ◽  
Alexandre Simões Dias ◽  
Marilene Porawski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Disease Onset ◽  
Treated Group ◽  
Diabetic Rats ◽  
Control Group ◽  
Agaricus Blazei ◽  
Pulmonary Tissue ◽  
Streptozotocin Induced Diabetes

The present study was designed to evaluate the oxidative stress as well as the therapeutic effect ofAgaricus blazeiMuril (A. Blazei) in rats with streptozotocin-induced diabetes. We used 25 Wistar rats, and DM was induced by injecting streptozotocin (70 mg/Kg i.p.).Agaricus blazeiMuril was administered daily starting 40 days after disease onset.A. Blazeiwas tested as an aqueous extract for its phytochemical composition, and its antioxidant activity in vitro was also evaluated. Lipoperoxidation (LPO), and superoxide dismutase (SOD), catalase, and glutathione peroxidase activities were measured in the pulmonary tissue, as well as the presence of inducible nitric oxide synthase (iNOS), through immunohistochemistry. An anatomopathologic study was also performed. Phytochemical screening ofA. Blazeidetected the presence of alkaloids and saponins. The extract exhibited a significant antioxidant activity in the DPPH-scavenging and the hipoxanthine/xanthine oxidase assays. Pulmonary LPO increased in diabetic animals (0.43±0.09;P<.001) as compared to the control group (0.18±0.02), followed by a reduction in theA. Blazei-treated group (0.33±0.04;P<.05). iNOS was found increased in the lung in diabetic rats and reduced in theA. Blazei-treated group. The pulmonary tissue in diabetic rats showed oxidative alterations related to the streptozotocin treatment. TheA. Blazeitreatment effectively reduced the oxidative stress and contributed to tissue recovery.

scholarly journals Effect of Extracts of Kigelia Africana Fruit and Sorghum Bicolor Stalk on the Biochemical Parameters of Alloxan-Induced Diabetic Rats

2021 ◽  
pp. 86-97
Author(s):  
Nkem F. Obianagha ◽  
Chukwuma J. Okafor ◽  
Ufuoma Chukwuani ◽  
Emmanuel Ifeanyi Obeagu ◽  
Omobolanle Abioye Ogundahunsi ◽  
...  
Keyword(s):  
Liver Function ◽  
Sorghum Bicolor ◽  
Plasma Glucose ◽  
Biochemical Parameters ◽  
Treated Group ◽  
Diabetic Rats ◽  
Hypoglycemic Agents ◽  
Control Group ◽  
World Population ◽  
Significant Difference

Diabetes and its complication continue to remain a concern in the world population despite the introduction of various hypoglycemic agents. Biochemical changes remain the major observable, clinical, and pathological factors as a derangement in the levels of these parameters increase the risk of developing complications. Although herbal extracts for the treatment of diabetes have been scientifically validated, the scientific interaction and alteration of the biochemical parameters due to the administration of this herbal product are not thoroughly investigated in this region. Therefore, this study aims to determine the effect of the extract of Kigeliaafricana and Sorghum bicolor stalk on the biochemical parameters of diabetic-induced rats. A total of eleven groups containing five rats per group of randomly selected Alloxan-induced Diabetes Healthy Wistar rats of both sexes were used in the study, with one serving as the control group, another serving as glibenclamide treated group, and the remaining nine, extract treated groups. After the overnight fast, the control group received a dose of 0.5 ml of 2% w/v acacia solution. Glibenclamide treated group received 600 μg/kg body weight (bwt) glibenclamide. In contrast, the other nine groups received specified doses (125, 250, and 500mg/kg bwt) of Kigeliaafricana and Sorghum bicolor extracts singly and in a mixture of ratio 1:1, respectively. After receiving the specified doses once a day orally for 30 days, the rats fasted overnight, and 5 ml of blood collected via cardiac puncture into heparinized and fluoride bottles. The samples were spun and separated for biochemical profiles (plasma glucose, lipid profile, liver function test, and electrolytes urea and creatinine) using a commercially prepared kit with outlined procedures. The result showed a significant decrease (p < 0.05) of plasma glucose level in the extract-treated rats, with a remarkable increase in untreated diabetic rats compared to the control. A significant reduction (p < 0.01) in the plasma levels of triglyceride and LDL-Chol was also observed in all treated groups at various doses of extracts compared to control with HDL-Chol values marginally increased in all extract-treated groups. Urea and creatinine showed a significant decrease (p < 0.01) in all treated doses except the lowest when compared to the control, while all the electrolytes parameters (Na+, K+, Cl- and HCO3-) show no significant difference (p > 0.05) across the groups when compared to the control. Liver function showed no significant differences in all parametersexcept for the noticeable decreasing effects on AST and ALT values compared to the control. The extracts and their mixture exhibited antidiabetic and hypolipidaemic activities and cardiovascular benefits due to their considerable lowering effects on total cholesterol, LDL-Chol, triglycerides, and increase in the HDL-Chol levels. The observed biological actions may be due to the presence of different phytocomponents present in the plant extracts. There is, therefore, a need to determine which of the active constituents has the main antidiabetic and hypolipidaemic effect for their optimal usage.

scholarly journals Resveratrol Reverses Thioacetamide-Induced Renal Assault with respect to Oxidative Stress, Renal Function, DNA Damage, and Cytokine Release in Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Seema Zargar ◽  
Mona Alonazi ◽  
Humaira Rizwana ◽  
Tanveer A. Wani
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Dna Damage ◽  
Creatine Kinase ◽  
Renal Function ◽  
Treated Group ◽  
Control Group ◽  
Cytokine Release ◽  
Kidney Toxicity ◽  
Significant Difference

Background. Thioacetamide (TAA), a class 2B-type carcinogen, is a potent toxicant. Toxicities caused by this compound in various tissues due to oxidative stress, increase of proinflammatory markers, and apoptosis have been reported; however, reports on kidney toxicity are negligible. Resveratrol (RSV), on the other hand, has demonstrated antioxidant and anti-inflammatory effects in different cases. Resveratrol’s protective effects against TAA kidney toxicity were investigated in four rat groups. Methodology. Four groups of rats were studied as follows (n=8): control group, where rats were fed normal diet and water; TAA group, where rats received 0.3% TAA in water for two weeks; RSV group, where rats received 10 mg/kg body weight (bw) of RSV as oral suspension for two weeks; and treated group, where rats orally received 10 mg/kg bw RSV and simultaneously received 0.3% TAA for two weeks. Kidney homogenates from all groups were analyzed for cytokine release (IL-4, TNF-α, and IFN-γ) and oxidative stress (lipid peroxidation, catalase, and 8-OHdG). The serum of rats was analyzed for the quantification of renal function markers (blood urea nitrogen (BUN), creatinine, and creatine kinase). Result. A significant increase in the renal function markers (BUN, 240%; creatinine, 187%; and creatine kinase, 117%), oxidative stress parameters (lipid peroxidation, 192% increase; catalase, 30.5% decrease), cytokines (IL-4, 120%; TNF-α, 129%; and IFN-γ, 133%), and DNA damage was observed in the TAA-treated group. All changes were significantly reversed in the group treated with RSV and TAA (P<0.05) in combination, with no significant difference compared to the control group. Conclusion. We conclude that resveratrol shows protection against TAA toxicity in rat kidney with respect to DNA damage, oxidative stress, renal function and cytokine release.

scholarly journals ETHNOPHARMACOLOGICAL STUDY OF BRAIN OXIDATIVE STRESS IMPROVING POTENTIAL OF CURCUMIN IN INTOXICATED RATS

2021 ◽  
pp. 62-66
Author(s):  
FATEN IBRAHIM EL-SAYED
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Brain Tissue ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Stress Effect ◽  
Stress Effects ◽  
Stress Markers ◽  
Group A

Objective: The following study aimed to investigate the efficacy of curcumin at preventing amikacin neurotoxicity Methods: Twenty-four male Wister albino rats were randomly divided into four groups including-G (1): control group includes six rats, they were administered 0.5 ml of saline orally for 14 consecutive days. G (2): includes six rats; they were administered 200 mg/kg curcumin orally for 14 consecutive days. G (3): includes six rats, they were administered 300 mg/kg body weight/day of amikacin intraperitoneally for 14 consecutive days G (4): includes six rats, they were administered 200 mg/kg curcumin orally concurrently with 300 mg/kg body weight/day of amikacin. All animals were kept in the same conditions from feed, heat and humidity. Results: According to the result obtained after sacrification of all animals after the end of 14 d, Results revealed that amikacin at the dose rate of 300 mg/kg b. wt for 14 d induces significant changes in oxidative stress markers compared to the control group, a significant reduction in CAT. SOD. GSH (1.51±0.16, 77.00±0.73 and 84.06±4.42) respectively compared to control (3.63±0.11, 98.48±0.18 and 117.05±0.52) along with a significant increase in MDA activity (219.02±3.34) compared to control group (180.42±0.19), That indicate oxidative stress effect of it. On the beneficial side rats received amikacin 300 mg/kg B. wt I/p concurrently with 200 mg/kg b. wt curcumin for successive 14day result in a significant increase in CAT. SOD. GSH (2.23±0.09,92.00±0.26, 102.25±1.71) and decrease in MDA concentration (139.23±3.89) compared to amikacin treated group levels along with histopathological changes appear in brain tissue in the group treated with amikacin include nuclear pyknosis and degeneration in some neurons in the hippocampus, multiple focal eosinophilic plaque formation in the striatum also this results enhanced by activated caspase-3 expression in the brain tissue following amikacin administration. Conclusion: The present study proved that Oral administration of curcumin at the dose of 200 mg/kg for 14 d concurrently with amikacin significantly mitigates its neurotoxic and oxidative stress effects.

scholarly journals Ethanolic Leaf Extract of Gymnema sylvestre Ameliorates Hyperglycemia and Pancreatic Oxidative Stress in Alloxan induced Diabetic Rats

2020 ◽  
Vol 11 (3) ◽  
pp. 426-432
Author(s):  
Kumud Ranjan Thakur ◽  
Shree Ram Radmadeo ◽  
Annpurna Kumari
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Indian Subcontinent ◽  
Hematological Parameters ◽  
Ethanolic Extract ◽  
Biochemical Parameter ◽  
Treated Group ◽  
Diabetic Rats ◽  
Gymnema Sylvestre ◽  
Anti Oxidant

Diabetes mellitus (DM) is a serious metabolic disorder with altered carbohydrate, fat, and protein metabolism. In the last four decades, India has emerged as an epicenter of the global diabetes mellitus pandemic. Rapid changes in the developmental scenario, demographic changes, and living style in the Indian subcontinent have led to the explosive increase in diabetes. Present research probes with ethanolic extract of Gymnema sylvestre (500 mg/kg.b.w) for treatment of hyperglycemia and related oxidative stress caused by Alloxan (100 mg/kg.b.wt), as a diabetogenic agent. 25 rats were included in the research divided into 5 groups, each containing 5 rats. Group 1 (normal rats), Group II (Diabetic rats (DM), Group III (DM+ treated for 10 days), Group IV (DM+20 days treated), Group V (DM+30 days treated). Blood samples and pancreatic tissues were collected at each interval of time. The blood sample was used for biochemical parameter and tissues were used for the anti-oxidant assay. Gymnema sylvestre extract (GSE) showed glucose-lowering property meanwhile, insulin secretion also increased as compared to Diabetic rats. Other tests like amylase, lipase, ALT, and AST also showed significant recovery after the extract administration. Oxidative stress was found in the Diabetic group, but after extract treatment concentration of superoxide dismutase, Glutathione-S-transferase, catalase, Glutathione peroxidase, Glutathione, and Total thiol was regained. Imbalance in serum electrolyte recovered and dysregulated hematological parameters due to stress and hyperglycemia showed convincing results. The finding suggests Gymnema sylvestre could be used as a hypoglycemic as well as an anti-oxidant agent in diabetes.

scholarly journals The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats

2019 ◽  
Author(s):  
Seddigheh Sheikh Hosseini ◽  
Ali Gol ◽  
Moje Khaleghi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Glutathione Peroxidase ◽  
Normal Saline ◽  
Lactobacillus Acidophilus ◽  
Diabetic Rats ◽  
Male Rats ◽  
Significant Difference ◽  
Anti Oxidant ◽  
The Impact

Background: Oxidative stress plays an important role in the development of diabetic complications. Objective: This study evaluated the impact of pre- and post-treatment with Lactobacillus acidophilus ATCC 4356 on the oxidant and anti-oxidant factors of testis and epididymis in streptozotocin-induced diabetic rats. Materials and Methods: Thirty male Wistar rats (10 wk old) weighing 220-230 g. were divided into five groups (n = 6/ each): 1- normal group, 2- normal lactobacillus group, 3- diabetic group, 4- diabetic + lactobacillus before (DLB) group, and 5- diabetic + lactobacillus after (DLA) group. The normal and diabetic groups received daily 1 mL normal saline for 6 wk. Normal lactobacillus group received daily L. acidophilus for 6 wk. Group DLB received daily L. acidophilus for 2 wk before diabetes and for 4 wk after diabetes. Group DLA received daily 1 mL normal saline for 2 wk before diabetes and L. acidophilus for 4 wk after diabetes. The dose of L. acidophilus was 1 × 109 CFU/mL. Results: The administration of L. acidophilus worsened blood glucose level and reduced the levels of Malondialdehyde (p = ≤ 0.0001) and Hydrogen peroxide (p ≤ 0.0001) and, Catalase and Glutathione peroxidase activity increased in the testis. In epididymis, Glutathione peroxidase and Catalase (p = 0.013) activity increased and Hydrogen peroxide concentration reduced, while Malondialdehyde concentration did not show any changes compared to the diabetic rats. Also, there was no significant difference between DLB and DLA groups, in these markers. Conclusion: Data obtained suggests that L. acidophilus has anti-oxidant effects on the testis and sometime in the epididymis in diabetic rats.

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