scholarly journals The Effect of Vitamin C on Lead-induced Plasma BiochemicalAlterations in Fish, Cyprinus carpio

2019 ◽  
Vol 13 (2) ◽  
pp. 25-29
Author(s):  
Kimiya Nourian ◽  
◽  
Hassan Baghshani ◽  
Davar Shahsavani ◽  
◽  
...  

Background: This study was conducted to investigate whether vitamin C can protect against plasma biochemical changes induced by lead poisoning in Cyprinus carpio. Methods: Three groups of common carp (n=30/group) were used in this study. Group 1 served as control, Group 2 was exposed to lead acetate (5 mg/L) for 15 days, and Group 3 received vitamin C (500 mg/kg) during the same duration of lead exposure. Results: The blood lead concentrations in Groups 2 and 3 showed a significant rise as compared to that in the control group (p<0.05). Also, the plasma malondialdehyde (MDA) concentration increased considerably following in groups exposed to lead acetate, compared to that in controls. Vitamin C supplementation decreased the rise in plasma MDA insignificantly, compared that in Group 2. The plasma values of creatinine and alanine aminotransferase (ALT) increased in Group 2 compared to that in controls. Also, vitamin C treatment significantly decreased plasma ALT and creatinine concentrations, compared to those in Group 2. Values of other plasma parameters including aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, gammaglutamyl transferase, albumin, glucose, total protein, cholesterol, urea, uric acid and triglyceride showed no significant alterations among the treatment groups. Conclusion: The results suggest that vitamin C have some beneficial effects against lead toxicity in common carp. However, elucidation of the precise mechanism of the protective effects of vitamin C against lead toxicity warrants further investigations

2011 ◽  
Vol 35 (1) ◽  
pp. 141-152
Author(s):  
Jamal Khalaf Atiyah AL-Faragi

One hundred and twelve Common carp fish fingerling Cyprinus carpio L.were collected, range between 25-28 gm in weight to study the effect of different concentrations of local probiotic and mixed with Vitamin C in concentration 1g/kg feed on growth performance, blood and biochemical picture. This experiment included 7 groups each group contain 16 fish distributed randomly as follow: Control group (C ) fed diet free vitamin C and probiotic during the period of 60 days and all total protin in feed reach 26% . Group 2(T1) group fed diet contain Vit C (1gm /kg) food. Treated (T2andT3) groups fed diet containing probiotic 4.5&5.5 g/ kg respectively , where as groupsT4,T5 and T6 fed the diets containing Probiotic added at level 4.5,5.5 and 6.5 g/ kg respectively and each mixed with 1 gm\kg Vit C .Results of fish total growth rate were 6.86,9.82,15.00,10.87,17.51,14.60 and 13.51gm, evaluate relative growth rate ,the absolute specific growth rate, food conversion and the protein efficiency ratio . The statistically analysis showed increase significantly(P≤0.05)for T4 in all treatment. While erythrocyte counts was 1.80, 1.88, 1.95, 2.40, 2.00, 2.70, 2.20 and 2.10 ×106 cells\mm3, blood cells counts are 24.40 ,24.60 ,25.80 ,27.00 ,26.00 ,27.96 ,26.60 and 26.20×103cells\mm3, blood haemoglobin was8.40,8.70,9.80, 11.10,10.20,11.90,10.80 and 10.50 gm\100dl, haematocrit value are 27.10 ,27.60 ,28.50 ,29.90 ,29.10 ,30.50 ,29.50 and29.30 % for fish before starting expermint (Z) and C,T1,T2,T3,T4,T5,T6,groups, respectively . Evaluate the enzymatic activity for AST,ALT and ALP in blood serum of fish . The statisticaly analysis showed decrease significantly(P≤0.05) at treatment contain mix probiotic and Vit C but excel T4 inform 70.40,10.40,30.30 I U\ L respectively.Conclusion ,the present studied suggested that the level of probiotic and Vit C (4.5g\kg and Vi C 1gm\kg) used could be improve the performance growth and fish healthy.


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.


Author(s):  
R. S. Ingole ◽  
A. S. Chandewar ◽  
M. V. Joshi ◽  
Madhuri Hedau ◽  
S W Hajare ◽  
...  

The effect of dietary lead exposure on immunomodulation of broilers and possible ameliorative effect of Andrographis paniculata were studied. One hundred broiler chicks were randomly divided into five equal groups of 20 each. Group T0 served as control while group T1, T2, T3 and T4 birds were provided commercial diet with lead acetate at the dose of 300 ppm in feed. Powder of dry leaves of Andrographis paniculata was mixed in the feed of group T2, T3 and T4 chicks at the dose of 2 gm, 3 gm and 4 gm/kg of feed respectively and fed for 4 weeks. Significant decrease in HI titres in T1 group and significant increase in T4 group suggested increased haemagglutination titre (HI) response. The maximum skin thickness and diameter on 24th and 48th hours was observed in control group birds (T0) and was comparable with T4 group. Microscopically spleen, thymus and bursa of fabricius revealed increase in lymphoid population in birds fed lead acetate along with Andrographis paniculata compared to T1 group. We concluded that Andrographis paniculata has immunomodulatory effect during lead toxicity in broilers.


2021 ◽  
Vol 219 ◽  
pp. 112318
Author(s):  
Sib Sankar Giri ◽  
Min Jung Kim ◽  
Sang Guen Kim ◽  
Sang Wha Kim ◽  
Jeong Woo Kang ◽  
...  

Author(s):  
Ewa Ziółkowska ◽  
Joanna Bogucka ◽  
Jan Mazurkiewicz ◽  
Mateusz Rawski ◽  
Szymon Różański ◽  
...  

AbstractCommon carp (Cyprinus carpio L.) is a dominant fish species in aquaculture, and as it is a stomachless species, absorption and digestion of nutrients take place in the intestine. The aim of the study was to evaluate the effects of a prebiotic on the content of selected minerals found in the meat, gills, and skeleton of common carp. The research applied trans-galactooligosaccharide (GOS) prebiotic produced by enzymatic transgalactosylation of milk lactose by whole cells of Bifidobacterium bifidum. The following diets have been applied: control diet without feed additives (C), diet 2 (B1) with 1% of GOS, and diet 3 (B2) with 2% of GOS. In the freeze-dried samples, concentrations of the analyzed metals were determined using atomic absorption spectroscopy (AAS). The content of phosphorus was determined using colorimetric method. The analyses confirmed that the highest level of Mg was detected in the skeleton of fish fed with 1% GOS (2.51 g kg−1) and was significantly higher compared the control treatment (2.11 g kg−1) (P < 0.05). Zn content in fish meat fed with 1% GOS (35.41 mg kg−1) was significantly higher (P < 0.05) than in the control group (24.59 mg kg−1). The tissue that accumulated the greatest amount of Zn was the gills. GOS had a positive effect on Fe accumulation in the meat, gills, and skeleton. It has been concluded that supplementation of feed with 2% GOS significantly influenced the positive correlations between Mg and P in the meat and skeleton, Fe–Ca correlation in gills, and Fe–Zn correlation in the skeleton.


PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0250885
Author(s):  
Zhaofang Chen ◽  
Kexin Shi ◽  
Wenjie Kuang ◽  
Lei Huang

Cadmium (Cd) is a toxic non-essential element, while calcium (Ca) is an essential element with high chemical similarity to Cd. Dietary intake is the major Cd exposure pathway for non-smokers. A multi-concentration dietary intervention experiment was designed to explore the optimum concentration of Ca in diet with obvious protective effects against the toxicity of livers and kidneys induced by Cd in mice. The mice were divided into six groups with different concentrations of Cd and Ca in their food: control-group (no Cd or Ca), Ca-group (100 g/kg Ca, without Cd), Cd-group (2 mg/kg Cd, without Ca), CaL+Cd-group (2 mg/kg Cd, 2 g/kg Ca), CaM+Cd-group (2 mg/kg Cd, 20 g/kg Ca) and CaH+Cd-group (2 mg/kg Cd, 100 g/kg Ca). The organ indexes, oxidative stress biomarkers, lesions and Cd concentrations were detected after a 30-day exposure period. Results showed that serum Aspartate Aminotransferase (AST) level in CaH+Cd-group was significantly lower than that in Cd-group, while close to that in control-group. The contents of Serum Blood Urea Nitrogen (BUN) in different groups showed the same trend. Concentrations of all oxidative stress biomarkers (GSH-Px, SOD, CAT, GSH and MDA) in CaH+Cd-group were close to the normal levels of control-group while significantly different from those in Cd-group. The only exception was the Malondialdehyde (MDA) levels in kidneys. This study suggests that Ca plays a protective role in relieving the Cd-induced toxicity of livers and kidneys and a concentration of 100 g/kg for Ca in diet showed the best protective effects. These findings could provide a clue for further studies concerning human diet intervention for Cd control.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Sarmad Ghazi Al-Shawi ◽  
Asraa Yacoob Yousif ◽  
Zena Kadhim Al-Younis ◽  
Rustem Adamovich Shichiyakh ◽  
Angelina Olegovna Zekiy ◽  
...  

Abstract The present study evaluated the protective effects of silymarin extract (SIE) on cadmium chloride toxicity in common carp, Cyprinus carpio. Four experimental group were considered for the experiment including: SIE0 (control): non-SIE-supplemented fish, SIE1: fish supplemented with 400 mg SIE/kg diet, SIE2: fish supplemented with1400mg SIE/kg diet, SIE3: fish supplemented with 2400 mg SIE/kg diet). Fish were fed experimental diet for 60 days and then exposed to cadmium chloride (1.5 mg/l or 25% of LC50–96 h) and antioxidant defense components and the survival rate assayed. After 60 days feeding trial, total antioxidant capacity (TAC) levels significantly increased (P<0.01) in 1400–2400 mg SIE/kg diet treatments compared to those in control and 400 mg SIE/kg diet treatment. Malondialdehyde (MDA) (P>0.01) and acetylcholinesterase (AChE) levels (P>0.01) remained unchanged during the feeding period in all treatments. Hepatic catalase (CAT) in all SIE supplemented groups and superoxide dismutase (SOD) and glutathione peroxidase (GPx) in 1400–2400 mg SIE/kg diet treatments significantly elevated (P<0.01) in response to SIE. Plasma levels of hepatic metabolic enzymes [alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine kinase (CK), lactate dehydrogenase (LDH) ] remained unchanged (P>0.01) in all experimental groups over feeding period. After exposure to cadmium chloride, TAC levels were significantly elevated (P<0.01) in all experimental groups. In control and 400 mg SIE/kg diet treatment, TAC showed lower levels (P<0.01) compared to other groups. MDA levels were significantly increased (P<0.01) in control and fish supplemented with 400 and 1400 mg SIE/kg diet. TAC in the fish of 2400 mg SIE/kg diet treatment remained unchanged (P>0.01), following the exposure. CAT (P<0.01), SOD (P<0.01) and GPx (P<0.01) were significantly elevated in response to cadmium chloride in all groups. However, the treatments, 1400 and 2400 mg SIE/kg diet, showed lower increases (P<0.01) of enzymes. AChE activity (P<0.01) in the liver were significantly decreased in control and fish fed 400 and 1400 mg SIE/kg diet. Exposure to cadmium significantly increased (P<0.01) the plasma levels of ALT, AST, ALP and LDH in control and those fed 400 and 1400 mg SIE/kg diet. The findings of the current study indicated that SIE at a rate of 1400–2400mg/kg diet might enhance antioxidant defense and protect hepatocytes against toxic effects of cadmium.


2021 ◽  
Vol 45 (2) ◽  
pp. 14-20
Author(s):  
Omar H Azeez

Aspartame (ASP) is a sugar substitute. Its use rose because it has been demonstrated to have deleterious effects after being metabolized. In the presence of antioxidant vitamins C or E, the effects of ASP on reproductive hormones of adult male and female Albino Wister rats were investigated. A total of eighty male and female rats were used in this study. The rats were divided into four groups: group 1, received no treatment; group 2, received ASP at 40 mg/kg BW; group 3, received ASP at 40 mg/kg BW with vitamin C at 150 mg/kg BW; and group 4, received ASP at 40 mg/kg BW and vitamin E at 100 mg/kg BW. All treatments were given orally by gavage needle once daily for consecutive 90 days. The levels of estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone hormone (TH) were measured after 90 days in blood plasma. In comparison with the control group, ASP treatment resulted in lower levels of E2, FSH, and LH in male and female rats. When the antioxidants vitamin C or E was given, the effects of ASP were reversed, and the levels of E2, LH, and FSH were increased. The testosterone hormone was likewise significantly increased by ASP, but testosterone hormone concentrations were decreased by vitamin C or E treatments. Long-term ASP consumption caused interfering with testicular and ovarian hormonal activity, while vitamins C and E on the other hand, overcome longstanding consumption ASP's effects.


2014 ◽  
Vol 38 (2) ◽  
pp. 128-137
Author(s):  
Jamal K. Al-faragi,

The aim of the present study was to assess the effect of a commercial ß-glucan against acute toxic effect of copper sulfate on common carp Cyprinus carpio L. behavioral, hematological parameters and biochemical tests. A total of 250 Fish (150 ± 2 g) were fed a basal control diet or the basal diet plus a ß-glucan (1 g kg-1 diet) for 60 days. LC50 was calculated for 72 h which was 10.83 mg L-1.  At the end of the trial, CuSO4.5H2O was added to all treatments  at a dose  of  5 mgL-1  for  T1  and  T2, 7mg L-1  for  T3  and  T4, 9  mgL-1 for T5  and  T6   for  96h. Fish behavioral changes were recorded during 96h of exposure to different Cu concentrations with and without β-glucan, which showed abnormalities such as increase swimming activity, jerky movement, loss of equilibrium, respiratory stress, increase operculum movement, frequent jumping, erratic swimming and swimming near the water surface. On the other hand, ß-glucan supplemented groups showed significant increase (P≤0.05) in Hb concentration, PCV%, RBC and WBC count in comparison with groups without ß-glucan. The results of dietary groups without ß-glucan showed a significant decrease (P≤ 0.05) in Hb concentration, hematocrit value, RBC count, but there were significant increase (P≤ 0.05) in WBC count with an increase of Cu concentration compared to control groups. Results showed significant increase (P≤ 0.05) in dietary groups without ß-glucan in values of ALT, AST and ALP enzymes activity with elevation of Cu levels compared to control group. But, ß-glucan supplemented groups showed significant decrease (P ≤ 0.05) in values of ALT, AST and ALP enzymes activity in comparison with dietary groups without ß-glucan. These results suggested that β-glucan has protective effect against CuSO4 toxicity and future studies should be pursued particularly immune response and molecular studies


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