scholarly journals Effects Of Some Locally Made Antiseptic Soaps on Some Skin Microbes

2021 ◽  
pp. 339-341
Author(s):  
Rabiu Lawan ◽  
Adam Idris
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Antifungal Activities ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Bacteria And Fungi

The antimicrobial activity of some local antiseptic soaps against some isolated microorganisms (bacteria and fungi) were carried out by agar diffusion method. The highest zone of inhibition was recorded to be 3cm at 100mg/ml by MK antiseptic soap against E.coli, while IJB and S.T antiseptic soaps show no significant effect against the skin isolates of the bacteria Staphylococcus and Streptococcus. So also the antifungal activities of the three soaps were tested and the highest zone of inhibition was 5cm at 100mg/ml by IJB local antiseptic soap against Malessezia fur fur and Tricophyton spp, while there is less effect (generally less than 4cm at 100mg/ml, 50mg/ml and 25mg/ml) of MK and S.T antiseptic soaps against the skin isolates of the fungi Malessezia furfur, Malessezia globossa, Microsporium spp and Tricophyton spp. This indicated that MK and IJB antiseptic soaps are potentially effective against bacteria and fungi respectively.

scholarly journals Investigation of Bulb Extracts of Crinum jagus for Antibacterial and Antifungal Activities

2021 ◽  
Vol 25 (1) ◽  
pp. 113-117
Author(s):  
T.T. Alawode ◽  
L. Lajide ◽  
B.J. Owolabi ◽  
M.T. Olaleye
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Antifungal Activities ◽  
Agar Diffusion ◽  
Antibacterial And Antifungal Activities ◽  
Successive Extraction ◽  
Antibacterial And Antifungal

This study investigates the bulb extracts of Crinum jagus for antimicrobial activities. The bulb samples were dried, ground and subjected to successive extraction using hexane, ethylacetate and methanol. The extracts were screened for activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Klebisidlae pneumonae, Candida albicans, Aspergillus niger, Penicillium notatum and Rhizopus stolonifer at concentrations between 6.25 and 200 mg/ml. Antimicrobial assays werecarried out using agar diffusion method. The Minimum Inhibitory Concentration (MIC) of the extracts was determined. The percentage yields obtained for the hexane, ethylacetate and methanol extracts of the bulbs are 0.28 %, 0.44% and 24.68 % respectively. Results showed that the methanolic extract had better antibacterial and antifungal activities than the other extracts. The methanol extract showed the highest antibacterial activity against S. aureus with a zone of inhibition of 28 mm at 200 mg/ml. Also, the methanol extract showed the highest antifungal activities against C. albicans, A. niger and P. notatum with a zone of inhibition of 20 mm at 200 mg/ml. The hexane and ethylacetate extracts had no activity against the microorganisms at 6.25 mg/ml.The methanol extract had the lowest Minimum Inhibitory Concentration (MIC) of 2.50 mg/ml against S. aureus, E. coli, B. subtilis, P. aeruginosa and S. typhi. The methanol extract could be a source of potent antimicrobial compounds. Keywords: Antibacterial, Antifungal, Crinum jagus, Agar diffusion, MIC

scholarly journals Evaluation of Antimicrobial Activity of Sida cordifolia Leaf Extract

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Dhanapal Venkatachalam ◽  
Samuel Thavamani ◽  
Aneesh C. Sebastian ◽  
V. B. Anju ◽  
Christy Mathew ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Fungus ◽  
Chloroform Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Sida Cordifolia ◽  
Antibacterial And Antifungal

Objective: The objective of the study was to evaluate in-vitro efficacy of antibacterial, and antifungal activities of chloroform extract of leaf of Sida cordifolia L. against some human pathogenic bacterial and fungal strains. S. cordifolia (Malvaceae) is used as anti-rheumatic, analgesic, antipyretic, anti-asthmatic, nasal decongestant, antiviral, laxative, diuretic, aphrodisiac, hypoglycemic, hepatoprotective and in the treatment of Parkinson disease. Methodology: Chloroform extract of S.cordifolia was used for antimicrobial screening. Antibacterial activity was tested against pathogenic bacterial strains Eschericha coli and Staphylococcus aureus. Also antifungal activity was tested against one human pathogenic fungal strain i.e. Candida albicans. Antibacterial and antifungal activities of S. cordifolia extract was carried out by using disc diffusion method. Results: Chloroform extract of S. cordifolia showed the largest zone of inhibition (10mm) against S. aureus at 50µgm/mL and no zone of inhibition against E.coli at 50µgm/ml. Chloroform extract has shown more potent antibacterial activity against S.aureus. Chloroform extract was found to be more effective against human pathogenic fungus C. albicuns. The zone of inhibition of chloroform extract was 10mm.The phytochemical screening demonstrated the presence of different types of compounds like alkaloids, tannins and phenolic compounds which may contribute for the anti-microbial action of this plant.  Conclusions: The results concludes that chloroform extract of S. cordifolia leaf possess antimicrobial activity.

scholarly journals PHYTOCHEMICAL SCREENING AND EVALUATION OF IN-VITRO ANTIMICROBIAL ACTIVITY OF DROSERA SPATULATA VAR BAKOENSIS- AN INDIGENOUS CARNIVOROUS PLANT AGAINST RESPIRATORY TRACT INFECTIOUS MICROBES.

2016 ◽  
Vol 9 (6) ◽  
pp. 274
Author(s):  
Chidepudi D S L N Tulasi
Keyword(s):  
Antimicrobial Activity ◽  
Respiratory Tract ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
Methanol Extracts ◽  
Low Concentrations ◽  
Bacteria And Fungi

Objective: In the present study, the phytochemical constituents of Drosera spatulata, have been evaluated and antimicrobial activity was screened against respiratory tract infectious microbes.Methods: The phytochemicals present in Drosera spatulata by qualitative phytochemical assays and the antimicrobial activity along with MIC, MBC and BIC were determined against S. aureus, Klebsiella pneumonia and S.Pneumonia the causative organisms of pulmonary infections, mainly effects the nasal pharynx, trachea, and lungs as well as Aspergillus niger.Results: Aqueous, ethanol, methanol extracts of thick roots, open flower and hair of Drosera spatulata var bakoensis against this pathogenic bacteria and fungi showed high zone of inhibition which estimated by disc-diffusion method as well as minimum inhibition concentration manifestation by the broth microdilution assay followed MBC and BIC. The values of MIC, MBC and BIC obtained were between 0.3-0.9, 0.36-2.25, 0.12 - 0.37 mg/mL. The results revealed that the plant extracts of Drosera spatulata var bakoensis have high potential even at low concentrations values against bacteria and fungi cultures and this results validated by the presence of high amounts of alkaloids, quinones, anthraquinones, flavonoids in the plant extracts. Conclusion: In the present study, the results showed the presence of high amounts of alkaloids, flavonoids, quninoes, anthraquinones, terpinoids in Drasera spatulata plant.  In the antibacterial and antifungal activity, the ethanol and methanol extracts significantly showed the activity against the tested respiratory disease causing bacteria and antifungal properties with zone of inhibition showed more than aqueous extracts at very low concentrations

scholarly journals AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT DARI AIR SUSU IBU (ASI) TERHADAP Listeria monocytogenes FNCC 0156

2020 ◽  
Vol 9 (1) ◽  
pp. 96
Author(s):  
Made Juli Antari ◽  
Ni Nyoman Puspawati ◽  
Putu Ari Sandhi Wipradnyadewi
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Breast Milk ◽  
Lactic Acid Bacteria ◽  
Bacteriocin Production ◽  
Diffusion Method ◽  
Contact Method ◽  
Agar Diffusion ◽  
Agar Diffusion Method

The aim of the research was to find the potency of Lactic Acid Bacteria isolated from breast milk on inhibiting Listeria monocytogenes FNCC 0156 and to find the magnitude of lactic acid bacteria from breast milk in inhibiting L. monocytogenes FNCC 0156. This reseach consists of two phase: antimicrobial activity of lactic acid bacteria from breast milk and activity of bacteriocin against L. monocytogenes FNCC 0156. Isolate used in this research was A1, A3, A6, A8, A9, B3, B7, B8, and B10b. Antimicrobial activity of lactic acid bacteria from breast milk against L. monocytogenes FNCC 0156 was performed cell free supernatant, cell free neutral supernatant, and cell free neutral heated supernatant. The test was performed by well-agar diffusion method and contact method. The activity of bacteriocin was carried out according to the optimum incubation time of bacteriocin production in cell free heated neutral supernatant. The highest antimicrobial activity was supernatant treatment by well-agar diffusion method and contact method showed by isolate A1 with inhibition diameter of 10.60 mm and a decrease of 1.66 log cycle. The highest antimicrobial activity was neutral supernatant treatment by well-agar diffusion method and contact method showed by isolate B8 with inhibition diameter of 5.11 mm and increased only by 0.18 log cycle. The highest antimicrobial activity on the heated neutral supernatant treatment by well-agar diffusion method and contact method showed by isolate A6 with inhibition diameter of 0.85 mm and a decrease of 0.48 log cycle. The two isolates of cell free neutral heated supernatant treatment was isolate A6 and isolate B8 suspected to have bacteriocin compounds was then continued with isolation of bacteriocin. The result of the second phase showed that optimum time for bacteriocin production of isolate A6 was 36 hours and B8 was 60 hours. The highest antimicrobial activity of bacteriocin was showen by isolate A6 with an average inhibition diameter of 5.36 mm, followed by B8 with an average inhibition diameter of 2.44 mm. Keywords: lactic acid bacteria, breast milk, Listeria monocytogenes FNCC 0156, antimicrobial activity, bacteriocin

scholarly journals Activity of Kefir grains against Gardnerella vaginalis

2020 ◽  
Vol 3 (4) ◽  
pp. 69-73
Author(s):  
Salwa Afifi ◽  
Amany El-Sharif ◽  
Zeinab Helal ◽  
Eman El-Musallamy
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Gardnerella Vaginalis ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Animal Trials ◽  
Agar Diffusion Method ◽  
Kefir Grains

Kefir is a probiotic mixture of bacteria and yeasts. In vitro and animal trials have shown kefir to have antibacterial, antifungal and antiviral activity. The main goal of this study was to determine the antimicrobial susceptibility of Gardnerella vaginalis (G.vaginalis) to kefir. Antimicrobial agents, which were commonly used to treat bacterial vaginosis, and kefir were both tested for antimicrobial activities against G.vaginalis using an agar diffusion method. Kefir was able to inhibit the growth of G.vaginalis. Kefir found to have antimicrobial activity similar to ampicillin, chloramphenicol, ceftriaxone, erythromycin and gentamicin against G.vaginalis isolates. Kefir produced zone of inhibition sizes higher than the zones produced by ciprofloxacin, sulphamethoxazole /trimethoprim and tetracycline. Special attention must be paid to kefir as its activity against G. vaginalis recommends that it deserves assessment in the treatment of infections involving G. vaginalis.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

scholarly journals Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

2016 ◽  
Vol 31 (2) ◽  
pp. 89
Author(s):  
Tatiana Dantas Piana ◽  
Maria de Fátima Malvar Gesteira ◽  
Erica Dos Santos Carvalho ◽  
Josilene Borges Torres Lima Matos ◽  
Monica Franca ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Effect ◽  
Antimicrobial Action ◽  
Diffusion Method ◽  
Agar Diffusion ◽  
Inhibition Zones ◽  
Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY OF ROOT EXTRACT OF CLITORIA TERNATEA

2017 ◽  
Vol 10 (11) ◽  
pp. 52
Author(s):  
Amita Shobha Rao ◽  
Shobha Kl ◽  
Prathibha Md’almeida ◽  
Kiranmai S Rai
Keyword(s):  
Antimicrobial Activity ◽  
Resistant Strain ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Root Extract ◽  
Alcoholic Extract ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
E Coli ◽  
Multidrug Resistant Strain

  Objective: Infections caused by Gram-negative bacteria are important causes of morbidity and mortality. Extracts of plants and herbs such as Clitorea ternatea are used as diuretic. This work attempts to find out antimicrobial activity of aqueous and alcoholic extract of C. ternatea roots against Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), clinical strains of Klebsiella pneumoniae, and Candida albicans.Methods: The agar well-diffusion method was done using Mueller Hinton agar and Sabouraud’s dextrose agar. The microorganism grown in peptone water was inoculated into culture medium. 4 mm diameter well punched into the agar was filled with 20 μl of aqueous and alcoholic root extracts C. ternatea extracts in various concentrations (100-25 μg/ml). The plates were incubated and antimicrobial activity was evaluated.Results: Aqueous root extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition against E. coli (ATCC 25922) 18 mm, P. aeruginosa (ATCC 27853) 14 mm, multidrug resistant strain of K. pneumoniae 15 mm. Alcoholic extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition of 35 mm against E. coli (ATCC 25922), P. aeruginosa (ATCC 27853) 22 mm, and multidrug resistant strain of K. pneumoniae 28 mm. C. albicanswas resistant to both extract of C. ternatea root. Conclusions: Alcoholic extract of C. ternatea is a better antibacterial agent against multidrug resistant Klebsiella species and other Gram-negative pathogens. Further, studies are required to identify active substances from the alcoholic extracts of C. ternatea for treating infections.

scholarly journals Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus

2013 ◽  
Vol 14 (5) ◽  
pp. 924-929 ◽  
Author(s):  
Reena Kulshrestha ◽  
J Kranthi ◽  
P Krishna Rao ◽  
Feroz Jenner ◽  
V Abdul Jaleel ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Antimicrobial Activity ◽  
Dental Health ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Test Organism ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method

ABSTRACT Aim The present study was conducted to evaluate the efficacy of commercially available herbal toothpastes against the different periodontopathogens. Materials and methods Six herbal toothpastes that were commonly commercially available were included in the study. Colgate herbal, Babool, Meswak, Neem active, Dabur red toothpastes were tested for the study whereas sterile normal saline was used as control. Antimicrobial efficacies of dentifrices were evaluated against Streptococcus mutans and Actinobacillus actinomycetemcomitans. The antimicrobial properties of dentifrices were tested by measuring the maximum zone of inhibition at 24 hours on the Mueller Hinton Agar media inoculated with microbial strain using disk diffusion method. Each dentifrice was tested at 100% concentration (full strength). Results The study showed that all dentifrices selected for the study were effective against the entire test organism but to varying degree. Neem active tooth paste gave a reading of 25.4 mm as the zone of inhibition which was highest amongst all of the test dentifrices. Colgate Herbal and Meswak dentifrices recorded a larger maximum zone of inhibition, measuring 23 and 22.6 mm respectively, compared to other toothpastes. All other dentifrices showed the zone of inhibition to be between 17 and 19 mm respectively. Conclusion The antibacterial properties of six dentifrices were studied in vitro and concluded that almost all of the dentifrices available commercially had antibacterial properties to some extent to benefit dental health or antiplaque action. How to cite this article Jenner F, Jaleel VA, Kulshrestha R, Maheswar G, Rao PK, Kranthi J. Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus. J Contemp Dent Pract 2013;14(5):924-929.

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