scholarly journals Comparative evaluation of methods for studying the degree of assimilation of feed by animals

2021 ◽  
pp. 25-29
Author(s):  
Yu. G. Belyakova ◽  
B. V. Usha

Relevance. The activity of the digestive organs is largely determined by the feeding regime, in particular, the frequency, timeliness, and the order of feeding the feed. The digestibility of nutrients is influenced by the preparation of feed for feeding, which facilitates mechanical processing in the digestive tract and the action of digestive enzymes. Against the background of a decrease in the natural resistance of the animal body, an important aspect is the use of mineral supplements and the enrichment of diets with biologically active substances that increase the nutritional value and the degree of assimilation of feed by animals. The aim of the work was a comparative assessment of methods for studying the degree of assimilation of feed by animals.Methods. To determine the degree of food assimilation, 2 drops of isotonic (0.9%) sodium chloride solution and a small amount of intestinal contents were applied to a glass slide, a thin smear was prepared, dried in air for 30 min, and large particles were removed. The preparations were fixed for 15 min with 96% ethyl alcohol, then 3–5 drops of a 1.0% aqueous solution of methylene blue, Lugol, Sudan III were applied. Along with the indicated conventional methods, we tested a method for preparing preparations using fixation in vapors of a 25.0% solution of glutaraldehyde for 30–40 minutes, in vapors of a 1.0% aqueous solution of osmium tetroxide.Results. The kinetics of the formation of intestinal microbiocenoses is characterized by a decrease in the number of lactobacilli and an increase in the number of pathogenic and opportunistic bacteria. In a comparative assessment of the preparation methods, the advantage of microscopic research methods is the possibility of a detailed study of the contents, the degree of assimilation of feed components in the gastrointestinal tract, and a visual assessment of the state of the intestinal microflora. The advantage of the method for preparing preparations with the use of vapor fixation of a 25.0% solution of glutaraldehyde and osmium tetroxide is the possibility of visualizing the formation of monospecific and polyspecies biofilms by optical and scanning electron microscopy. The preparations were sufficiently contrasting, there were no artifacts, bacteria and fungi stained brown, which made it possible to count even pinpoint colonies using a magnifying glass or an optical microscope. The easily digestible feed results in a low fecal yield and a stable consistency. The use of functional food “Pristine Gold Vet” in the nutrition of dogs and fur-bearing animals made it possible to increase the absorption of nutrients by 18,1% and 15,3%.

Symmetry ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 497
Author(s):  
Ewa D. Raczyńska

Keto-enol prototropic conversions for carbonyl compounds and phenols have been extensively studied, and many interesting review articles and even books appeared in the last 50 years. Quite a different situation takes place for derivatives of biologically active azulene, for which only scanty information on this phenomenon can be found in the literature. In this work, quantum-chemical studies have been undertaken for symmetrically and unsymmetrically substituted azulenols (constitutional isomers of naphthols). Stabilities of two enol (OH) rotamers and all possible keto (CH) tautomers have been analyzed in the gas phase {DFT(B3LYP)/6-311+G(d,p)} and also in aqueous solution {PCM(water)//DFT(B3LYP)/6-311+G(d,p)}. Contrary to naphthols, for which the keto forms can be neglected, at least one keto isomer (C1H, C2H, and/or C3H) contributes significantly to the tautomeric mixture of each azulenol to a higher degree in vacuo (non-polar environment) than in water (polar amphoteric solvent). The highest amounts of the CH forms have been found for 2- and 5-hydroxyazulenes, and the smallest ones for 1- and 6-hydroxy derivatives. The keto tautomer(s), together with the enol rotamers, can also participate in deprotonation reaction leading to a common anion and influence its acid-base properties. The strongest acidity in vacuo exhibits 6-hydroxyazulene, and the weakest one displays 1-hydroxyazulene, but all azulenols are stronger acids than phenol and naphthols. Bond length alternation in all DFT-optimized structures has been measured using the harmonic oscillator model of electron delocalization (HOMED) index. Generally, the HOMED values decrease for the keto tautomers, particularly for the ring containing the labile proton. Even for the keto tautomers possessing energetic parameters close to those of the enol isomers, the HOMED indices are low. However, some kind of parallelism exists for the keto forms between their relative energies and HOMEDs estimated for the entire molecules.


2021 ◽  
Vol 35 ◽  
pp. 205873842110005
Author(s):  
Xia Ma ◽  
Meng Yang ◽  
Yan He ◽  
Chuntao Zhai ◽  
Chengliang Li

Tremella polysaccharide is known to be structurally unique and biologically active natural products, abundant and versatile in activities and applications in food industry, daily chemical industry and medicine industry. In order to improve the industrialisation of Tremella polysaccharide, the limitations of preparation and structure-activity relationship of Tremella polysaccharide were reviewed in this paper. The research progress of Tremella polysaccharide in the past 20 years was summarized from the sources, preparation methods, molecular structure, activity and application, and the research trend in the future was also prospected. The application prospect of Tremella polysaccharide in against multiple sub-health states was worth expecting.


Author(s):  
B.G. Tsugkiev ◽  
◽  
L.Ch. Gagieva ◽  
T.V. Tsugkieva ◽  
M.M. Chegaev ◽  
...  

The paper presents a comparative assessment of the nutritional value depending on the phenological phase of Onobrychis viciifolia Scop., as well as the determination of the regularity of the accumulation of biologically active substances and heavy metals in the herb of the species. The yield of green mass of plants was determined. From each hectare of sowing, 9,7 centners of protein can be obtained; the yield of fiber is 13 centners, BEV is 22,9 centners, fat is 1,6 centners, ash is 4 centners and carotene is 13.0 kg. Depending on the growing season, the chemical composition in the grass of Onobrychis viciifolia changes. The content of calcium, phosphorus increases, and the presence of iron, copper, cobalt, zinc, manganese and molybdenum and amino acids decreases.


Author(s):  
Mirvari Hasanova Mirvari Hasanova

The separation and purification of antibiotics with sorption by ion-exchange materials, as well as their delivery in biological processes by immobilization, are now widely used in biotechnology. There are many scientific studies in the literature on the sorption of antibiotics by polymer-based sorbents and inorganic materials, as well as the study of thermodynamics and kinetics of the process. In the literature, the acquisition of biologically active systems from the sorption of antibiotics by ion-exchange fibers based on various polymers and inorganic substances was carried out. However, the synthesis of selective gels for the effective separation of doxycycline and its delivery in different pH mediums by sorption with biodegradable, biocompatible polysaccharide-containing composites is one of the topical issues. Gel was synthesized from the cross-linking of N,N-diethyl N-methyl derivative of a natural polyaminosaccharide of chitosan by glutaric aldehyde. Also, pH-sensitive hydrogels that can swollen in water were synthesized from the cross-linking of a graft copolymer of cherry source gummiarabic with N-vinylpyrrolidone, as well as synthetic polymer polyacrylic acid with N,N-methylene-bis-acrylamide. The structure of the gels were identified by FTIR and NMR spectroscopy, and the sorption of doxycycline antibiotic from an aqueous solution was investigated. According to the values of zeta potential, the protonation of functional groups in the main macromolecule in an acidic medium leads to a value of zeta potential of 40÷80 mV on the surface of chitosan-based gel and others. Although the chemical structure is different, the isoelectric point is set around pH=6÷8 for all three hydrogels. The dependences of the sorption process on the amount of gels, antibiotic concentration, temperature, and pH medium were studied. The experimental data were analyzed using two adsorption models, Langmuir and Freundlich, with the later system providing the best fit. Doxycycline is adsorbed on the surface of chitosan, gummiarabic and polyacrylic acid based hydrogel composite through by physical interactions. Also, the results of thermodynamic parameters ΔG40 kJ/mol show that the nature of the adsorption process is physical, and spontaneous, too. Keywords: Chitosan, Gummiarabic-arabinogalactane, polyacrylic acid, hydrogel, sorption isoterms, doxycycline, thermodynamica.


Author(s):  
С. В. Шершова

Проведено експериментальне обґрунтування біо-конверсії відходів вирощування (полови) ехінацеїпурпурової (Echinacea purpurea (L.) Moench.) таехінацеї блідої (Echinacea pallida (Nutt) Nutt.).Доведена висока активність екстрактів полови зехінацеї пурпурової, що проявлялася у стимуляціїросту тест-культури. Встановлено, що найвищустимулюючу активність мають екстракти поло-ви ехінацеї пурпурової: водні за концентрації0,01 %, спиртові – 0,01–0,001 %; до того ж іззбільшенням концентрації спирту в екстрактахзнижувалась їх активність. Вперше встановлено,що полова ехінацеї містить специфічні білки –лектини, активність яких у відходах ехінацеї блі-дої значно перевищувала ехінацею пурпурову. Роз-роблено технологічні схеми отримання лектинівта екстрактів біологічно активних речовин із від-ходів вирощування (полови) для їх ефективноговикористовування. The experimental foundation bioconversion of waste product of growing (chaff), purple coneflower(Echinacea purpurea (L.) Moench.) and pale coneflower (Echinacea pallida (Nutt.) Nutt.) was to carriedout. The high activity of extracts of coneflower chaff, which had growth-stimulating effect to the testculture, was proved. Found that the highest stimulating activity has the extract of chaff purpleconeflowers: aqueous solution at concentrations of 0.01 %, alcohol-0.01 %-0.001 % and with increasingconcentration of alcohol in the extracts decreased their activity. For the first time it has been shown thatchaff of Echinacea contains specific proteins - lectins, and their activity in the waste product of paleconeflower significantly exceeded then activity in the waste product of purple coneflower. Technologicalschemes to get of lectins and extracts of biologically active substances from waste growing product(chaff), was worked out for their effective use.


2015 ◽  
Vol 799-800 ◽  
pp. 125-129
Author(s):  
Mary Donnabelle L. Balela ◽  
April Alexa S. Lagarde ◽  
Stephen Jann A. Tamayo ◽  
Nikko S. Villareal ◽  
Ann Marielle Parreno

Zirconia (ZrO2) nanotubes were synthesized by anodization of zirconium (Zr) foil in NH4Fand (NH4)2SO4 aqueous solution. Different surface preparation methods (electropolishing and etching) were applied on the Zr foil prior to anodizaton. In addition, the anodization time and NH4F concentration were varied. The structure and morphologies of the nanotubes and their crystallinity were confirmed using scanning electron microscope and x-ray diffractometer, respectively. ZrO2 nanotubes with large diameters and thick walls were formed at lower NH4F concentration and longer anodization time. On the other hand, smaller nanotubes with thinner walls were produced when the NH4F concentration was increased. The synthesized nanotubes were predominantly tetragonal ZrO2 with small amounts of monoclinic ZrO2.


1994 ◽  
Vol 358 ◽  
Author(s):  
Georges Denes ◽  
P. Kabro ◽  
M.C. Madamba

ABSTRACTHematite Fe2O3, is a semiconductor, and its electrical properties are highly sensitive to the preparation methods and purity. Its precursors can be hydrated ferric oxides; however, these are usually obtained from poorly defined ferric gels, which are obtained by hydrolysis of an aqueous solution of a ferric salt by a base. We have designed a novel synthetic route to ferric hydroxide, by reaction of a peroxo-compound with an aqueous solution of a ferrous salt, which involves simultaneous oxidation of Fe(ll) to Fe(lll), and hydrolysis, in the same reaction process. The two kinds of ferric hydroxide are highly different, however, both give hematite by dehydration/recrystallization, however, the way this occurs for each is different.


2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Ravindra P. Singh

Organic conducting polymer modified electrodes (OCPMEs) have emerged as potential candidates for electrochemical biosensors due to their easy preparation methods along with unique properties, like stability in air and being compatible with biological molecules in a neutral aqueous solution. OCPMEs are playing an important role in the improvement of public health and environment for the detection of desired analytes with high sensitivity and specificity. In this paper, we highlight the prospects of OCMEs-based electrochemical enzymosensors.


2015 ◽  
Vol 814 ◽  
pp. 441-446
Author(s):  
Wei Xing Chen ◽  
Xiao Fang Gao ◽  
Ai Jie Ma

The removal of Fe3+, Cu2+,Cd2+,Cr3+and Zn2+ions from aqueous solution was studied by sorption onto polystyrene microcapsules containing 8-hydroxy-quinoline as extraction reagent. The micro-capsules containing 8-hydroxy-quinoline were prepared by solvent evaporation method with O/W emulsion and characterized by using optical microscope, Fourier Transform Infrared Spectroscopy (FT-IR). The uptake to Fe3+, Cu2+,Cd2+,Cr3+and Zn2+ions into the microcapsules was studied at deferent conditions. It showed that with the increasing dosage of microcapsules, the removal of metal ions increased too. The longer is standing time, the more is the increasing of adsorption capacity. The sorption of metal ions decreases with the initial pH of the solution increasing, and the sorption of microcapsule for metal ions is lower at 50°C. The sorption of Cr3+, Cu2+and Zn2+ions is highest at 45°C, and that of Fe3+, Cd2+is at 40°C. The results showed that the microcapsules could absorb Cr3+ions better than other metal ions.


Author(s):  
Г.В. Худов ◽  
І.А. Хижняк

The article discusses the methods of swarm intelligence, namely, an improved method based on the ant colony optimization and the method of an artificial bee colony. The goal of the work is to carry out a comparative assessment of the optical-electronic images segmentation quality by the ant colony optimization and the artificial bee colony. Segmentation of tonal optical-electronic images was carried out using the proposed methods of swarm intelligence. The results of the segmentation of optical-electronic images obtained from the spacecraft are presented. A visual assessment of the quality of segmentation results was carried out using improved methods. The classical errors of the first and second kind of segmentation of optoelectronic images are calculated for the proposed methods of swarm intelligence and for known segmentation methods. The features of using each of the proposed methods of swarm intelligence are determined. The tasks for which it is better to use each of the proposed methods of swarm intelligence are determined.


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