scholarly journals Відходи вирощування представників роду ехінацея (Echinacea Moench) як перспективне джерело біологічно активних речовин

Author(s):  
С. В. Шершова

Проведено експериментальне обґрунтування біо-конверсії відходів вирощування (полови) ехінацеїпурпурової (Echinacea purpurea (L.) Moench.) таехінацеї блідої (Echinacea pallida (Nutt) Nutt.).Доведена висока активність екстрактів полови зехінацеї пурпурової, що проявлялася у стимуляціїросту тест-культури. Встановлено, що найвищустимулюючу активність мають екстракти поло-ви ехінацеї пурпурової: водні за концентрації0,01 %, спиртові – 0,01–0,001 %; до того ж іззбільшенням концентрації спирту в екстрактахзнижувалась їх активність. Вперше встановлено,що полова ехінацеї містить специфічні білки –лектини, активність яких у відходах ехінацеї блі-дої значно перевищувала ехінацею пурпурову. Роз-роблено технологічні схеми отримання лектинівта екстрактів біологічно активних речовин із від-ходів вирощування (полови) для їх ефективноговикористовування. The experimental foundation bioconversion of waste product of growing (chaff), purple coneflower(Echinacea purpurea (L.) Moench.) and pale coneflower (Echinacea pallida (Nutt.) Nutt.) was to carriedout. The high activity of extracts of coneflower chaff, which had growth-stimulating effect to the testculture, was proved. Found that the highest stimulating activity has the extract of chaff purpleconeflowers: aqueous solution at concentrations of 0.01 %, alcohol-0.01 %-0.001 % and with increasingconcentration of alcohol in the extracts decreased their activity. For the first time it has been shown thatchaff of Echinacea contains specific proteins - lectins, and their activity in the waste product of paleconeflower significantly exceeded then activity in the waste product of purple coneflower. Technologicalschemes to get of lectins and extracts of biologically active substances from waste growing product(chaff), was worked out for their effective use.

Author(s):  
С. В. Поспелов

За багаторічними дослідженнями ехінацеї пурпурової (Echinacea purpurea (L.) Moench,) сорту Зірка Миколи Вавилова та ехінацеї блідої (Echinacea pallida (Nutt.) Nutt.) сорту Красуня Прерій вперше розроблені й запатентовані методи визначення продуктивності рослин прегенеративного періоду онтогенезу. Методики засновані на регресійних моделях із високими коефіцієнтами детермінації, на підставі яких можна без пошкодження рослин провести оцінку продуктивності надземної частини та кореневої системи протягом веґетаційного періоду. Вихідними даними для розрахунків слугують показники довжини і ширини листковоїпластинки, їх кількість, а також сума температур вище 5 0С і кількість діб від сівби. On the basis of long-term researches of Purple Coneflower (Echinacea purpurea (L.) Moench) variety «Zirka Mykoly Vavylova» and Pale Coneflower (Echinacea pallida (Nutt.) variety «Krasunja Preriy» for the first time ever there were developed and patented the methods for determining the efficiency of plants in pregenesic period of ontogeny. The foundation of methodology was made on the studies of regression models with high coefficients of determination which allow to make the estimation of aerial parts and root system productivity for the whole growing season without damaging the plants. The input data for the calculation are the indicators of the length and width of the leaf blade, the amount and the sum of temperatures above + 5 ºC and the number of days from sowing.


2018 ◽  
Vol 7 (3.14) ◽  
pp. 445 ◽  
Author(s):  
Elena Victorovna Ulrikh ◽  
Rafik Shamilovich Khaliullin ◽  
Irina Alexandrovna Ganieva ◽  
Ekaterina Aleksandrovna Izhmulkina ◽  
Mikhail Nikolayevich Arzjutov

Medicinal plants are producers of many biologically active substances – compounds that can influence biological processes in animals; such compounds include cardiac glycosides, saponins, sterols, carotenoids, polyphenols, alkaloids, vitamins, quinones, as well as substances with specific aroma, taste and color. Objects of the research were the extracts of purple coneflower (Echinacea purpurea), maral root (Leuzea carthamoides), holy thistle (Silybum marianum) and marigold (Calendula officinalis) obtained by low-temperature water-ethanolic extraction followed by low-temperature vacuum drying of the extracts. The content of biologically active substances was determined in accordance with the standard methods of chemical analysis for phytobiotic substances. As shown by the results of the studies, phytobiotic substances contain ascorbic acid, polysaccharides, tannins, carotenoids, phytosterols, fructose and inulin. They also contain many flavonoids, which, presumably, efficiently alter the economic-useful qualities of agricultural animals and poultry. The phytobiotical feed additives may indirectly control the intestinal microflora, supporting the internal protective mechanisms of the animal organism. The use of phytobiotical feed additives in the diet of farm animals and poultry is rationally substantiated, and contributes to abandoning the use of antibiotics in the fodder. 


Author(s):  
С. В. Поспєлов

В умовах Лісостепу України досліджено законо-мірності цвітіння ехінацеї пурпурової (Echinaceapurpurea (L.) Moench), сорту Зірка Миколи Вавило-ва й ехінацеї блідої (Echinacea pallida (Nutt.) Nutt.)сорту Красуня прерій. Вперше за багаторічнимиданими у виробничих умовах встановлено, що ехі-нацея пурпурова починає утворення суцвіть ізтравня, масове квітування – з липня. Одна рослиназа сезон утворює 48,2 шт. кошиків масою 184,25 г.Період квітування кошика першого порядку три-ває в середньому 34 доби, інших порядків – 33–43доби. Загальний період квітування рослини –близько 65 діб. Ехінацея бліда починає утвореннясуцвіть у травні, масове квітування у червні. Рос-лина за сезон утворює в середньому 10,4 шт. су-цвіть масою 30,1 г. Кошик першого порядку квітуєдо 15 діб, інших порядків – 19–29 діб. Загальнийперіод цвітіння рослини триває 34–37 діб. Дослі-дження дають змогу враховувати біологічні особ-ливості в разі конструювання лікарських, кормовихі медоносних агроценозів ехінацеї, планування збо-ру надземної маси на лікарську сировину, регуляціїпродуктивності й тривалості цвітіння. The peculiarity of flowering of purple coneflower (Echinaceapurpurea (L.) Moench) cultivar «Zirka Mykoly Vavilova» (Star ofNikolai Vavilov) and pale coneflower Echinacea pallida (Nutt.)Nutt.) variety «Krasunya preriy» (Prairie’s Beauty) in the foreststeppe of Ukraine have been studied. For the first time according tomany years’ data in a production environment it has beenestablished that the inflorescences formation of purple coneflowerbegins in May, massive flowering – in July. One plant per seasonforms 48.2 inflorescence with the mass of 184.25 g. The period offlowering inflorescence of the first-order lasts an average for 34days, other orders – 33–43 days. One plant blooms up to 65 days.Pale coneflower begins the formation of inflorescences in May, amassive bloom – in June. The plant per a season forms 10.4inflorescence with the mass of 30.1 g. Inflorescence of the firstorder flowers up to 15 days, other orders – 19–29 days. The totalperiod of flowering plants lasts for 34–37 days. Research ofbiological peculiarities of flowering should be considered in thedesign of medical, food and melliferous agrocenosis of Echinacea.Also at that time of planning harvesting the surface mass formedicinal raw materials, regulating the efficiency and duration offlowering.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 518d-518
Author(s):  
W. Letchamo ◽  
V.S. Krutilina

Echinace from the Asteracea family has been among the top 10 marketed herbs in North America and Europe. So far, only a limited amount of Echinace originate from commercial cultivation. Echinacea purpurea has been commercially cultivated in the United States since the mid 1970s, in Russia since 1935, while in Europe since the 1960s. However, there has been no published information in the English language on methods of cultivation, expected yields, limiting factors, nutrient requirements, and the optimum plant development stage to obtain maximum yield and concentration of the biologically active substances for pharmaceutical and cosmetic processing. The objective of this investigation was, therefore, to study the general distribution of major nutrients and biologically active substances and the relationship between the two groups, and the pattern of the accumulation of cichoric acid, echnacoside, isobutylamides (tetraenes) and essential oil in commercially cultivated Echinacea purpurea. There was a significant variation in the content of essential oil, cichoric acid, and isobutylamides (tetraenes) in different organs and stages of the plant development. Similarly, the difference in the distribution of N, P, K, Ca, Mg, and Fe in different organs and developmental stages is demonstrated. The correlation between the content of the active substances and nutrients is demonstrated, while recommendations for an optimum harvesting stage and date are made.


2021 ◽  
Vol 38 ◽  
pp. 00033
Author(s):  
Tatiana Fomina ◽  
Tatiana Kukushkina

The content of the major groups of biologically active substances in flowers of 7 Campanula species, cultivated in the forest-steppe of Western Siberia, were investigated for the first time. Freshly collected flowers were found to contain 0.06-0.15 % of catechins, 0.31-3.17 % of flavonols, 3.31-6.47 % of tannins, 3.10-15.57 % of pectic substances, and 0.66-16.4 mg% of carotenoids calculated per absolute dre weight of raw material. The amount of ascorbic acid was 36.9-114.0 mg% per wet weight. The data indicate that Campanula flowers are promising for the production of phenolic compounds, the development of food additives and fresh consumptions a seasoning.


2018 ◽  
Vol 1 (1) ◽  
pp. 11 ◽  
Author(s):  
Kostadinka B. Karneva ◽  
Ivelina N. Vasileva ◽  
Petko N. Denev ◽  
Rositsa S. Denkova ◽  
Vasil T. Shikov ◽  
...  

Bulgaria became the leading producer of lavender oil in the last years. Due to the lower quantity of essential oil large amounts of waste were generated which distilleries usually discard, although the residues are rich source of biologically active substances. The objective of this study was to obtain polyphenol-rich extracts from lavender waste and to investigate their chemical composition and antioxidant capacity. Two wastes (Mirkovo, Bulgaria, 2016) were investigated – steam distilled (SD-L) and CO2-extracted lavender (CO2-L). The major aroma constituents found were linalool and linalyl acetate – 30.68% and 25.82%, respectively, and the highest concentrations were found in the SD-L. The total flavonoids in CO2-L and SD-L were 2.91±0.11 and 3.72±0.20 mg/g dry matter residue, respectively. The higher amount of phenolic acids was observed in the SD-L waste – 2.62±0.19 mg/g, compared to 1.39±0.14 mg/g dry matter residue for CO2 extracted lavender. The antioxidant activity of the extracts was investigated by DPPH (SD-L – 355.48±23.12 µmol TE/g DW waste; CO2-L – 283.21±17.04 µmol TE/g DW waste) and FRAP (SD-L – 427.36±26.54 µmol TE/g DW waste; CO2-L – 311.29±18.17 µmol TE/g DW waste). Both methods suggested that SD-L residue had higher antioxidant capacity and polyphenol content.The lavender wastes (SD-L and CO2-L) showed strong antioxidant capacity with potential beneficial effect on addition in foodstuffs. For the first time lavender residues from CO2-extraction was investigated for its antioxidant activity, polyphenol composition and aroma metabolites, and comparison with SD-L was performed. In general, the results suggested that the lavender waste were promising source of antioxidants.


2019 ◽  
pp. 84-92 ◽  
Author(s):  
Alexandra Zaushintsena ◽  
Irina Milentyeva ◽  
Olga Babich ◽  
Svetlana Noskova ◽  
Tatyana Kiseleva ◽  
...  

Immunodeficiency causes a lot of modern diseases. Immunodeficiency, in its turn, is caused by such fac- tors as polluted environment, chronic stress, sedentary lifestyle, unbalanced diet, etc. All these factors weaken respi- ratory organs and gastrointestinal tract, disturb hormonal regulation, and destabilize immune defence. Food industry responds to these challenges by developing functional foods and dietary supplements from medicinal plants. Dietary supplements made from natural plant extracts have more advantages than their numerous synthetic analogues. They produce a mild therapeutic effect and no pronounced side effects. Purple Echinacea (Echinacea purpurea L.) posses- ses immunomodulatory, anti-inflammatory, antiviral, and tonic properties. However, climatic and soil conditions are known to affect the qualitative and quantitative profile of biologically active substances. The present paper describes the micronutrient profile of various parts of Echinacea purpurea grown in the Kemerovo region. The study employed a complex of physical and chemical methods. The research featured leaves, roots, and flowers, as well as components extracted from the plant with the help of a 70% ethanol solution. The latter was chosen for its universal properties in micronutrient extraction. The methods included high performance liquid chromatography (HPLC), thin layer chro- matography (TLC), and IR spectroscopy. A set of triple experiments showed that the extracts contained substances with anti-inflammatory, antioxidant, and immunomodulating properties. Thus, Echinacea extract can be recommen- ded for functional foods and dietary supplements.


Author(s):  
М. G. Markova ◽  
Е. N. Somova

Work on the clonal micropropagation of strawberries comes down to the search for new growth regulators, which include a biologically active substance - the waste product of the wax moth Galleria mellonella L. The effect of the waste product of the wax moth on the efficiency of clonal micropropagation of strawberries (Fragaria х ananassa duch) in vitro and in vivo conditions in 2018-2020 is shown. The object of research is micro-cuttings, rooted micro-cuttings and adapted micro-plants of garden strawberries of the Korona variety and of the remontant strawberries of the Brighton variety. It was revealed that at the proliferation stage, the propagation coefficient of the Korona variety increased significantly with the introduction of the waste product of the wax moth in doses of 4.0 mg/L and 6.0 mg/L and amounted to 4.2 and 3.8 pcs./explant, respectively; for Brighton variety, the coefficient increased significantly when the dose of the waste product of the wax moth 2.0 mg/L and amounted to 4.6 pcs./explant. The introduction of the waste product of the wax moth in doses of 4.0 mg/L and 6.0 mg/L into the nutrient medium had a significant effect on the yield of Brighton micro-cuttings suitable for rooting: the yield was 95.5 and 94.1%, respectively 87.7% in the control. For the Korona variety, no significant positive effect of the waste product of the wax moth on this indicator was noted. The rooting of micro-cuttings of strawberries of both varieties significantly increased with the introduction of the waste product of the wax moth into the nutrient medium in all studied doses and amounted to 86.4-100% in the Korona variety, and 88.9-100% in the Brighton variety.  The survival rate of adaptable micro-cuttings of Corona variety strawberries when sprayed with an aqueous solution of the waste product of the wax moth at a dose of 4.0 mg/L was 100%; the maximum survival rate of micro-cuttings Brighton variety is 99.8% in the variant with spraying with an aqueous solution of the waste product of the wax moth at a dose of 6.0 mg/L.


2021 ◽  
pp. 175-182
Author(s):  
Elena Sergeyevna Filonenko ◽  
Larisa Nikolayevna Zibareva

In this paper Silene graefferi Guss. is recommended for the first time as a promising source of flavonoids and is a well-known producer of ecdysteroids. This perennial species is adapted to the conditions of Western Siberia and has been successfully cultivated in the Siberian Botanical garden of TSU since 2015. It was interesting to study the composition and features of accumulation of biologically active substances during the growth and development of S. graefferi. It was shown that 20-hydroxyecdysone and polypodine B are present in the aerial and underground parts of introduced in culture S. graefferi plants during the entire vegetative development. An unidentified ecdysteroid was detected in the aerial part at the beginning of the vegetation and in the roots during the flowering phase. During vegetative development, common flavonoids and characteristic ones at different periods of plant development were found. The phase of maximum accumulation of ecdysteroids and flavonoids of S. graefferi is the beginning of vegetation, and the minimum is flowering. In the age dynamics the highest levels of biologically active substances in plants were noted at 2 and 3 years of life, then there is a general tendency to decrease with the age of the plant. From the aerial part of S. graefferi, 20-hydroxyecdysone, polypodine B and vitexin were isolated and identified by HPLC.


Author(s):  
С.О. СЕМЕНИХИН ◽  
О.В. ФЕДОСЕЕВА ◽  
М.В. БАБАКИНА ◽  
Н.М. ДАИШЕВА

Проведены исследования обработки растворов свекловичной мелассы 4 штаммами микроорганизмов с целью обогащения растворов жирами. Выявлено влияние начального значения рН, а также оптимального содержания сухих веществ в растворах мелассы, обеспечивающие эффективную жизнедеятельность микроорганизмов с синтезом биологически активных веществ. Установлено, что штаммы R. glutinis Y-332, C. curvatus Y-2236 и U. isabellina F-526 в растворах мелассы с начальным значением рН 8,5 синтезировали жиры в количестве 0,015 0,017 и 0,007 г/г сахаров соответственно штамм R. toruloides Y-334 в растворе мелассы с начальным значением рН 7,0 синтезировал жиры в количестве 0,004 г/г сахаров. Впервые получены экспериментальные данные, позволившие оценить эффективность микробиологического синтеза жиров в растворах мелассы без дополнительного внесения биогенных веществ, а также без поддержания оптимальной рН среды 4 видов микроорганизмов. Обосновано, что штаммы R. glutinis Y-332 и C. curvatus Y-2236 являются наиболее перспективными для обогащения растворов свекловичной мелассы жирами. Research aimed at treatment of beet molasses solutions with 4 strains of microorganisms in order to enrich the solutions with fats has beed established. The influence of the initial pH value, as well as the optimal dry matter content in molasses solutions, which ensure the effective vital activity of microorganisms with the synthesis of biologically active substances, is revealed. It was established that the strains of R. glutinis Y-332, C. curvatus Y-2236 and U. isabellina F-526 in molasses solutions with an initial pH of 8,5 synthesize fats in an amount of 0,015 0,017 and 0,007 g/g of sugars, respectively and the strain of R. toruloides Y-334 in molasses solution with an initial pH value of 7,0 synthesizes fats in an amount of 0,004 g/g of sugars. For the first time, it was obtained the experimental data that made possible to evaluate effectiveness of microbiological synthesis of fats in molasses solutions without nutrient addition, as well as without maintaining the optimal pH of the environment for 4 types of microorganisms. It is proved that R. glutinis strain Y-332 and C. curvatus strain Y-2236 are the most promising strains for enriching beet molasses solutions with fats.


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