scholarly journals Effect of Nano herbal Haramounting (Rhodomyrtus tomentosa) in Histology of Heart Mus musculus

2019 ◽  
Vol 1 (2) ◽  
pp. 101-106
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Research Use ◽  
Treatment Groups ◽  
High Energy Milling ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the effect of histology of heart Mus musculus  after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the  Completely Randomized Design (CRD). Treatment consists of 6 groups, namely:  Control: CMC 0.5%; T1:100mg/20gBW; T2: 141,42mg/20gBW; T3:200mg/20gBW; T4: 282,82mg/20gBW;  T5:400mg/20gBW. The results of histology hearts that there were significant difference (P<0,05) between  control group and treatment groups but there was not significantly different in weight of hearts (P>0,05).

scholarly journals Description of Kidney Histology of Mus Musculus After Giving Nano Hebal Rhodomyrtus tomentosa (Haramounting)

2019 ◽  
Vol 1 (1) ◽  
pp. 26-33
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Significant Difference ◽  
Proximal Tubular ◽  
Completely Randomized Design ◽  
Paraffin Method ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the description of kidney histology of Mus musculus after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the Completely Randomized Design (CRD), which consists of six groups of CMC 0.5% and Nano- R.tomentosa (Haramonting) at dosage of (100; 141,42; 200; 282,82; 400)mg/20g body weight. The kidneys were made into preparations by using the paraffin method and Hematoxylin Erlich-Eosin staining (HE). The results of histological kidneys showed that there is a significant difference (P<0,05) between control group and treatment groups with damaged cells and increased proximal tubular narrowing in kidneys, but there was not significantly different in weight of kidneys (P>0,05).

scholarly journals Histology of Spleen after Induction Nanoherbal Rhodomyrtus tomentosa

2020 ◽  
Vol 2 (1) ◽  
pp. 70-74
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Phenolic Compounds ◽  
Essential Fatty Acids ◽  
High Energy ◽  
Control Group ◽  
Lung Weight ◽  
Treatment Groups ◽  
Randomized Design ◽  
Significant Difference ◽  
Health Promoting ◽  
Rhodomyrtus Tomentosa

The study aim to know to effect of Nanoherbal Rhodomyrtus tomentosa (Haramonting) on spleen histology of Mus musculus. Haramonting in Indonesia as a new source of health-promoting compounds such as dietary fibers, essential fatty acids, and phenolic compounds because there are 19 phenolic compounds including stilbenes and ellagitannins as major components, followed by anthocyanins, flavonols, and gallic acid. Haramonting is made on a nanoscale using High Energy Milling (HEM). This research use the Completely Randomized Design (CRD) at dosage of (100; 141,42; 200; 282,82; 400)mg/20g body weight. The lungs were made into preparations by using the paraffin method and Hematoxylin Erlich-Eosin staining (HE). There were a significant difference (P<0,05) between control group and treatment groups on form of lung cells although no significant in lung weight

scholarly journals Effect of Nanoherbal Haramonting (Rhodomyrtus tomentosa) on Lungs Histology

2020 ◽  
Vol 2 (1) ◽  
pp. 43-47
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Phenolic Compounds ◽  
Essential Fatty Acids ◽  
High Energy ◽  
Control Group ◽  
Lung Weight ◽  
Treatment Groups ◽  
Randomized Design ◽  
Significant Difference ◽  
Health Promoting ◽  
Rhodomyrtus Tomentosa

The study aim to know to effect of Nanoherbal Rhodomyrtus tomentosa (Haramonting) on lungs histology of Mus musculus. Haramonting in Indonesia as a new source of health-promoting compounds such as dietary fibers, essential fatty acids, and phenolic compounds because there are 19 phenolic compounds including stilbenes and ellagitannins as major components, followed by anthocyanins, flavonols, and gallic acid. Haramonting is made on a nanoscale using High Energy Milling (HEM). This research use the Completely Randomized Design (CRD) at dosage of (100; 141,42; 200; 282,82; 400)mg/20g body weight. The lungs were made into preparations by using the paraffin method and Hematoxylin Erlich-Eosin staining (HE). There were a significant difference (P<0,05) between control group and treatment groups on form of lung cells although no significant in lung weight

scholarly journals Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan

2021 ◽  
Vol 9 (2) ◽  
pp. 79
Author(s):  
Febriyono Anggela Prakoso ◽  
Raden Tatang Santanu Adikara ◽  
Kusnoto Kusnoto ◽  
Sri Hidanah ◽  
Yeni Dhamayanti ◽  
...  
Keyword(s):  
Growth Rate ◽  
Weight Gain ◽  
Research Methods ◽  
Control Group ◽  
Treatment Groups ◽  
Gain Rate ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Bali Cattle

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.

scholarly journals PENGARUH PERASAN UMBI AKAR LOBAK (Rhapanus sativus L) TERHADAP PENINGKATAN KADAR PROLAKTIN DALAM DARAH MENCIT (Mus musculus) BETINA

2006 ◽  
Vol 11 (2) ◽  
pp. 135-137
Author(s):  
Mas Loegito ◽  
Paulus Liben ◽  
Thin Soedarti
Keyword(s):  
Mus Musculus ◽  
Prolactin Level ◽  
Day Treatment ◽  
Control Group ◽  
Treatment Groups ◽  
Female Mice ◽  
Randomized Design ◽  
Significant Difference ◽  
Per Oral ◽  
Tuber Extract

The objective of this research was to prove that the administration of radish tuber extract (Rhapanus sativus L) to female mice (Mus musculus) might increase blood prolactin level. This was an experimental research using complete randomized design by involving three treatment groups and three control group, each with five replications. Materials consisted of radish tuber extract and 30 female mice of 8 weeks old divided into 6 groups, i.e., control groups of C1, C2, and C3, and treatment groups of T1, T2, and T3. C1, C2, and C3 groups were given water of 1x 0.5 cc/day per oral, 2x0.5 cc/day, and 3x0.5 cc/day respectively. Treatment groups of T1, T2, and T3 were given radish tuber extract of respectively 1x0.5 cc/day per oral, 2x0.5 cc/day, and 1x0.5 cc/day. Treatment was carried out for 21 days. Blood was subsequently taken from those rats for blood prolactin level. Data were analyzed using Anove test. If the results indicated significant difference of 95 percent (p = 0.05), the analysis was continued with LSD. The conclusions of this experiment is the administration of radish tuber extract per oral may result in the increase of blood prolactin level.

scholarly journals PENGARUH AIR REBUSAN DAUN HARENDONG BULU (Clidemia hirta) TERHADAP KADAR GULA DARAH MENCIT (Mus musculus)

2020 ◽  
Vol 3 (1) ◽  
pp. 7-12
Author(s):  
Okta Malia ◽  
Dian Samitra ◽  
Eka Lokaria
Keyword(s):  
Blood Sugar ◽  
Mus Musculus ◽  
Design Research ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Sugar Levels ◽  
Boiled Water ◽  
One Way Anova

This study aims to determine the effect of the water boiled leaves of Clidemia hirta on blood sugar levels in Swiss Webster mice (Mus musculus). This research using a completely randomized design research design (CRD) with 5 treatment groups namely; Aquades control group (P0), 3 g sucrose treatment (P1), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.13 ml/KgBB (P2), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.26 ml/KgBB (P3), 3 g treatment of sucrose + boiled bristle leaves boiled water at a dose of 0.39 ml/KgBB (P4). Before administration of the extract, mice in the treatment groups P1, P2, P3 & P4 were induced using 3 g of sucrose for 5 days. Then after 5 days for groups P2, P3, and P4 followed by giving boiled water leaves for 7 days. The analysis result of One Way Anova Test shows that Fcount > Ftable (18.86 > 2.51). This shows the boiled water affects the blood sugar levels of male Swiss Webster mice. The concentration of extract at P4 (0.39 ml/KgBB) was more effective in reducing blood sugar levels.

scholarly journals DESCRIPTION OF TESTIS HISTOLOGY OF MUS MUSCULUS AFTER GIVING NANO HERBAL RHODOMYRTUS TOMENTOSA (HARAMONTING)

2018 ◽  
Vol 11 (11) ◽  
pp. 460 ◽  
Author(s):  
Putri Cahaya Situmorang ◽  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Test Results ◽  
Male Mice ◽  
High Energy Milling ◽  
Testis Histology ◽  
Significant Difference ◽  
Rhodomyrtus Tomentosa

Objective: The objective of this study was to determine the description of testis histology of male mice (Mus musculus) after giving nano-Rhodomyrtus tomentosa (haramonting).Methods: Leaves of R. tomentosa (haramonting) are made into nanoherbal using high-energy milling, treatment of mice in 14 days, then observation of testis histology, and the data processed with SPSS 22 program with Kruskal–Wallis test. Results: Nano-R. tomentosa administration was not significantly different in weight of testes (p>0.05), but there was significant difference in Spermatogonia, Spermatocytes, and spermatid.Conclusions: Nano-R. tomentosa (haramonting) with excessive doses can cause damage of testis cells, interfere with the process of spermatogenesis, and make infertile.

scholarly journals PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 169
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Virid Gibson ◽  
Alfiah Hayati
Keyword(s):  
Molecular Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Control Group ◽  
Protein Profiles ◽  
Testosterone Levels ◽  
Randomized Design ◽  
Sds Page ◽  
Elisa Technique ◽  
Completely Randomized Design

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of  15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of  60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>

scholarly journals Efek Serbuk Kunyit dan Kurkumin pada Spermatogenesis Mencit (Mus musculus) yang Diberi Minuman Beralkohol

2021 ◽  
Vol 6 (2) ◽  
pp. 154-160
Author(s):  
Helena Chika Valencia Hanisa ◽  
Tyas Rini Saraswati ◽  
Silvana Tana
Keyword(s):  
Analysis Of Variance ◽  
Mus Musculus ◽  
Alcoholic Beverages ◽  
Seminiferous Tubules ◽  
Treatment Groups ◽  
Alcohol Control ◽  
Randomized Design ◽  
Testicular Weight ◽  
Significant Difference ◽  
Spermatogonia Cells

Kunyit mengandung senyawa kurkumin yang dapat digunakan sebagai zat antiinflamasi dan membantu memperbaiki sel-sel yang rusak. Tujuan dari penelitian ini menganalisis pengaruh serbuk kunyit dan kurkumin pada jumlah dan ukuran sel spermatogonium; spermatosit primer; dan spermatosit sekunder; bobot testis serta diameter tubulus seminiferus Mus musculus yang diberi minuman beralkohol. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan 12 ekor Mus musculus jantan yang dibagi kedalam 4 kelompok perlakuan dan 3 kali ulangan. R0 merupakan kontrol, R1 kontrol alkohol, R2 pemberian serbuk kunyit sebanyak 0,1 mg/hari, R3 pemberian kurkumin sebanyak 0,01 mg/hari. Perlakuan diberikan selama 30 hari. Data penelitian dianalisis menggunakan analysis of variance (ANOVA) pada taraf kepercayaan 95%. Berdasarkan hasil penelitian yang diperoleh dapat disimpulkan bahwa tidak terdapat perbedaan bermakna (p>0.05) pada jumlah spermatogonium dan ukuran sel (spermatogonium, spermatosit primer, dan spermatosit sekunder), namun terdapat perbedaan bermakna pada (P<0,05) pada bobot testis, diameter tubulus seminiferus dan jumlah sel (spermatosit primer, dan spermatosit sekunder). Turmeric contains curcumin compounds that can be used as anti-inflammatory substances and help repair damaged cells. The purpose of this study was to analyze the effect of turmeric powder and curcumin on the number and size of spermatogonia cells; primary spermatocytes; and secondary spermatocytes; testicular weight and diameter of the seminiferous tubules of Mus musculus given alcoholic beverages. This study is an experimental study with a completely randomized design (CRD), using 12 male Mus musculus which were divided into 4 treatment groups and 3 replications. R0 is control, R1 is alcohol control, R2 is 0.1 mg/day of turmeric powder, R3 is 0.01 mg/day of curcumin. The treatment was given for 30 days. The research data were analyzed using analysis of variance (ANOVA) at the 95% confidence level. Based on the results obtained, it can be concluded that there is no significant difference (p>0.05) in the number of spermatogonia and cell size (spermatogonia, primary spermatocytes, and secondary spermatocytes), but there is a significant difference (P<0.05) in testicular weight, diameter of the seminiferous tubules and the number of cells (primary spermatocytes, and secondary spermatocytes).

scholarly journals Ujitoksisitasakut ekstrakbatangpisangambon(Musa paradisiacavar sapientum) terhadap hati mencit (Mus musculus) dengan parameter LD (Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD 50 parameters 50

2014 ◽  
Vol 13 (2) ◽  
pp. 86
Author(s):  
Hendrik Setia Budi ◽  
Ira Arundina ◽  
Retno Indrawati ◽  
Leonita Widyana Mahardikasari
Keyword(s):  
Wound Healing ◽  
Mus Musculus ◽  
Toxicity Test ◽  
Control Group ◽  
Treatment Groups ◽  
Musa Paradisiaca ◽  
Significant Difference ◽  
Post Test ◽  
Stem Extract ◽  
Banana Stem

The sap of ambonese banana (Musa paradisiaca var sapientum) contain flavonoids, saponins, tannins which have beenwidely used by people in Trunyan Bali as traditional medicine on wound healing, and it has been reported as apotential wound healing after tooth extraction. The aim of this research was to determine the level of safety of usingherbal medicine, ambonese banana stem extract on histopatology liver of mice with LDparameters. This experimentalstudy was performed bythe post test only controlled group design. The sample were 28 mice (Mus musculus) randomlydivided into 4 groups. K group as control group was given aquadest. P1, P2, and P3, as treatment groups were givenambonese banana stem extract with dose 0.42g/20gbw, 2.1g/20gbw, 4.2g/20gbw. The extract was given per-oral withsonde on the first day. On day 3, the mice were terminated, and the livers were microscopically histopathological observed. The observed at 3th50 day, there were no deaths in every groups of mice (K, P1, P2, and P3) on the third day observation. Kruskal Wallis test showed there was not significant difference in histopathological appearance on liverof mice (p=0.771). It wasconcluded that the maximumsafety dose that can still be administered is 0.42g/20gbw followingthe LDparameter. Theacute toxicity test of ambonese banana stem extract did not show necrosis on liver but it showedthe highest simple degeneration than all groups which were given 0.42g/20gbw dose.

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