scholarly journals DESCRIPTION OF TESTIS HISTOLOGY OF MUS MUSCULUS AFTER GIVING NANO HERBAL RHODOMYRTUS TOMENTOSA (HARAMONTING)

2018 ◽  
Vol 11 (11) ◽  
pp. 460 ◽  
Author(s):  
Putri Cahaya Situmorang ◽  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Test Results ◽  
Male Mice ◽  
High Energy Milling ◽  
Testis Histology ◽  
Significant Difference ◽  
Rhodomyrtus Tomentosa

Objective: The objective of this study was to determine the description of testis histology of male mice (Mus musculus) after giving nano-Rhodomyrtus tomentosa (haramonting).Methods: Leaves of R. tomentosa (haramonting) are made into nanoherbal using high-energy milling, treatment of mice in 14 days, then observation of testis histology, and the data processed with SPSS 22 program with Kruskal–Wallis test. Results: Nano-R. tomentosa administration was not significantly different in weight of testes (p>0.05), but there was significant difference in Spermatogonia, Spermatocytes, and spermatid.Conclusions: Nano-R. tomentosa (haramonting) with excessive doses can cause damage of testis cells, interfere with the process of spermatogenesis, and make infertile.

scholarly journals Effect of Nano herbal Haramounting (Rhodomyrtus tomentosa) in Histology of Heart Mus musculus

2019 ◽  
Vol 1 (2) ◽  
pp. 101-106
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Research Use ◽  
Treatment Groups ◽  
High Energy Milling ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the effect of histology of heart Mus musculus  after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the  Completely Randomized Design (CRD). Treatment consists of 6 groups, namely:  Control: CMC 0.5%; T1:100mg/20gBW; T2: 141,42mg/20gBW; T3:200mg/20gBW; T4: 282,82mg/20gBW;  T5:400mg/20gBW. The results of histology hearts that there were significant difference (P<0,05) between  control group and treatment groups but there was not significantly different in weight of hearts (P>0,05).

scholarly journals Egg Albumin Supplementation Decreases Isolation-Induced Aggression in Male White

2015 ◽  
pp. 70-80
Author(s):  
Francis Legario ◽  
Maureen Antonette Bereber
Keyword(s):  
Aggressive Behavior ◽  
Mus Musculus ◽  
Negative Control ◽  
Test Results ◽  
Male Mice ◽  
Egg Albumin ◽  
Homologous Genes ◽  
Significant Difference ◽  
Status Analysis ◽  
Different Levels

There is a need to search for solutions of minimizing aggression so as to minimize violence. The purpose of the study is to determine the effects of different levels of egg albumin supplementation to the aggressive behavior of male white mice (Mus musculus). Specifically, this study aimed to describe aggressive tendencies by isolation-induced fighting of male white mice when fed different levels of egg albumin; determine aggressive behavior in terms of the number of attacks and body scars when fed different levels of egg albumin; and measure dominant-subordinate status when fed different levels of egg albumin. White mice were chosen as test animals since they have homologous genes coding with humans. Furthermore, aggression tends to be more common among male mice than female mice. T-test results showed that there is a significant difference in the aggressive behavior between the treated and untreated mice in all treatments except for Treatment D (negative control) in terms of number of attacks and body scars and a significant difference in all treatments in terms of the dominant-subordinate status. Analysis of Variance (ANOVA) showed a significant difference in the number of attacks and the dominant subordinate status. Overall, results showed that egg albumin supplementation reduced aggression in male white mice regardless of the level of egg albumin. It was concretely shown that treatments fed with varied levels of egg albumin significantly alleviated aggressive behavior compared with treatments without egg albumin supplementation.

scholarly journals Description of Kidney Histology of Mus Musculus After Giving Nano Hebal Rhodomyrtus tomentosa (Haramounting)

2019 ◽  
Vol 1 (1) ◽  
pp. 26-33
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Significant Difference ◽  
Proximal Tubular ◽  
Completely Randomized Design ◽  
Paraffin Method ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the description of kidney histology of Mus musculus after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the Completely Randomized Design (CRD), which consists of six groups of CMC 0.5% and Nano- R.tomentosa (Haramonting) at dosage of (100; 141,42; 200; 282,82; 400)mg/20g body weight. The kidneys were made into preparations by using the paraffin method and Hematoxylin Erlich-Eosin staining (HE). The results of histological kidneys showed that there is a significant difference (P<0,05) between control group and treatment groups with damaged cells and increased proximal tubular narrowing in kidneys, but there was not significantly different in weight of kidneys (P>0,05).

scholarly journals Effect of Ethanol Extract and Ethyl Acetate Fraction of Betel Nut (Areca catechu L.) in Colonic Goblet Cells of Mice (Mus musculus) Given Orally Infective Egg of Trichuris muris

2020 ◽  
Vol 8 (B) ◽  
pp. 637-642
Author(s):  
Endy Juli Anto ◽  
Aznan Lelo ◽  
Syafruddin Ilyas ◽  
Marline Nainggolan
Keyword(s):  
Ethyl Acetate ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Goblet Cells ◽  
High Dose ◽  
Betel Nut ◽  
Male Mice ◽  
Trichuris Muris ◽  
Significant Difference

BACKGROUND: Trichuris trichiura is a soil-transmitted parasitic worm commonly found in humid, tropical to subtropical areas, as well as poor sanitation area. These worms are cosmopolitan, especially in tropical and humid countries like Indonesia. This worm infection is more common in developing countries and more in children than adults due to poorer child self-hygiene. Worm disease is one of the common society diseases with 60% of children in Indonesia which are estimated to be affected. One species of T. trichiura worm that is often used in research on experimental animals is Trichuris muris. The administration of T. muris infective eggs can significantly increase the number of goblet cells in intestinal crypts of colon and cecum. Goblet cell hyperplasia will occur after exposure to high-dose T. muris (acute/200 infective eggs) in resistant mice. Various studies on the development of natural and traditional materials have been developing in the past few years to see the effects of betel nut on the number of goblet cells in the large intestine. AIM: The aim of this research is to determine the effect of ethanol extract and the ethyl acetate fraction of betel nut on the change in the number of goblet cells in male mice given orally T. muris infective eggs. METHODS: This study was an experimental study with a post-test only control group design in male mice (Mus musculus) which were given 200 infective eggs of T. muris. The study sample consisted of 70 mice divided into seven groups. Doses of the ethanol extract of betel nut are 100 mg/kg b.w. and 150 mg/kg b.w., and doses of ethyl acetate fraction are 100 mg/kg b.w. and 150 mg/kg b.w. The independent variable was ethanol extract and ethyl acetate fraction of betel nut. The dependent variable is the number of goblet cells. Mean differences in groups were tested by Mann–Whitney. RESULTS: Statistical analysis showed a significant difference of p < 0.05 in the colonic crypts and cecum. In Group K (+), there was no increase in the number of goblet cells (54.2000 ± 30.54864) compared to Group K (−) (79.0750 ± 11.79221) in the colon. There was an increase in goblet cells in the 150 mg/kg b.w. of ethanol extract group (64.1000 ± 29.50381), the 150 mg/kg b.w. of ethyl acetate fraction group (56.2250 ± 22.11035), and the 1 mg/20 g of albendazole group (68.3750 ± 11.30956) in the colon. Likewise, there was an increase in the number of goblet cells in the cecum. It can be concluded that 150 mg/kg b.w. of the ethanol extract of betel nut can effectively increase the number of goblet cells in the colon and cecum. CONCLUSION: Ethanol extract of betel nut 150 mg / kg of weight can effectively increase the number of goblet cells in the colon and caecum.

scholarly journals PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum) TERHADAP DIAMETER DAN TEBAL EPITEL TUBULUS SEMINIFERUS MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN

2020 ◽  
Vol 8 (2) ◽  
pp. 132
Author(s):  
Aldis Ingrid Rusyawardani ◽  
Widjiati Widjiati ◽  
Suzanita Utama ◽  
Chairul Anwar ◽  
Epy Muhammad Luqman ◽  
...  
Keyword(s):  
Single Dose ◽  
Mus Musculus ◽  
Seminiferous Tubules ◽  
Average Weight ◽  
Male Mice ◽  
Treatment Groups ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Biophytum Petersianum ◽  
Epithelium Thickness

The aim of this research is to analyze the effect of kebar grass on the diameter and epithelium thickness of seminiferous tubules. Twenty male mice of BALB/C strain with an average weight of 25 gram were used in this research. Total five experimental groups (4 mice each group) were treated with combination of TCDD and kebar grass extract designed as follows: (C-) 0mg/g BW/day dan 0µg/kg BW, (C+) 0mg/g BW/day and 7µg/kg BW, (T1) 0,045mg/g BW/day and 7µg/kg BW, (T2) 0,080mg/g BW/day and 7µg/kg BW, (T3) 0,1350mg/g BW/day and 7µg/kg BW. The TCDD was injected peritoneally in single dose while kebar grass extract was given orally everyday for 53 days. The results showed a significant difference (p<0,05) on group which is treated with kebar grass extract 0,135mg/ g BW/ day is proven could maintain the diameter of seminiferous tubules in T3 group (170,5μmb ± 0,65μm) compared with C+ group (129,50μma ± 8,42μm). Significant difference of epithelial thickness were also found between the control and treatment groups (p<0,05). The C+ group (33,57μmb ± 1,61μm) was different from the T1 group (43,80μmc ± 1,46μm), T2 (48,49μmd ± 1,08μm), T3 (52,15μme ± 0,86μm) and C- (56,17μma ± 1,35μm). This findings might suggest TCDD has the potential to decrease the epithelium thickness and the diameter of seminiferous tubules, while kebar grass extract in various dose were proven to be able maintain the epithelium thickness of seminiferous tubules but could not maintain the diameter of seminiferous tubules.

scholarly journals EFEK PEMBERIAN L-ARGININ TERHADAP GAMBARAN HISTOLOGI JUMLAH SPERMATOSIT PRIMER PADA MENCIT (Mus musculus) SETELAH TERPAPAR SUHU PANAS

2020 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
Dirga Januar Surya Utama ◽  
Suherni Susilowati ◽  
Tatik Hernawati ◽  
Erma Safitri ◽  
Sri Mulyati ◽  
...  
Keyword(s):  
Body Weight ◽  
Data Analysis ◽  
High Temperature ◽  
Analysis Of Variance ◽  
Adult Male ◽  
Mus Musculus ◽  
Primary Spermatocyte ◽  
Male Mice ◽  
Randomized Design ◽  
Significant Difference

The purpose of this study was to know the effect of L-arginine on the histology of primary spermatocyte count in mice (Mus musculus) after high temperature exposed. The subjects of this study were 20 adult male mice, 8 weeks old with an body weight range from 20-40 grams. This research conducted by using Complete Randomized Design (RAL) with 4 treatments and 5 replications. The treatments consisted of, P0- = treatment with 1ml of aquabidest without high temperature exposed, P0 + = treatment with 1ml of aquabidest after high temperature exposed for 1hour per-day for 35 days, P1 = 1.3mg / day L-arginine dissolved in 1ml aquabidest and given orally after high temperature exposed for 1hour per-day for 35 days and P2 = 2.6mg / day L-arginine dissolved in 1ml aquabidest given orally after high temperature exposed for 1hour per-day for 35 days. Observations done by making histologic preparations of testicular organs and then calculated the total number of primary spermatocyte per treatment. The data of primary spermatocyte calculated and analyzed by using Analysis of Variance (ANOVA), followed by Duncan test. The result from data analysis showed that there was a significant difference (p<0.05) between P1 and P2 with control,between P1 and P2 the analysis did not show any significant  difference (p> 0.05).

scholarly journals KAJIAN EFEKTIVITAS EKSTRAK GEL IKAN TOMAN (Channa micropeltes) PADA LUKA BAKAR MENCIT (Mus musculus)

2019 ◽  
Vol 15 (2) ◽  
pp. 184
Author(s):  
Firlianty Firlianty ◽  
Hermansyah Hermansyah ◽  
Windarina Samosir
Keyword(s):  
Data Analysis ◽  
Experimental Method ◽  
Mus Musculus ◽  
Control Treatment ◽  
Test Results ◽  
Male Mice ◽  
In Vivo Test ◽  
Randomized Design ◽  
Completely Randomized Design

This study aims to determine the study of the effectiveness of toman fish (Channa micropeltes) gel extract on healing burns of mice (Mus musculus). This research was conducted for 4 months, starting from September 2018 to January 2019. This study used the experimental method and data analysis using a completely randomized design with 4 children and three replications: treatment A as control, treatment B giving toman fish extract gel (Channa micropeltes ) 1.5%, treatment C 3% and treatment D 4.5%. The results showed that on day 4, day 8 and day 12 showed that the administration of gel extract had a significant effect on changes in the length of burns of mice (Mus musculus) (P>0.05). In vivo test results on male mice with toman fish gel extract can capture the healing of burns in mice.

scholarly journals Oral subchronic toxicity of (+)-2,2’epicytoskyrin a on male mice (Mus musculus)

2021 ◽  
Vol 33 ◽  
pp. 06007
Author(s):  
Ni Luh Putu Rischa Phadmacanty ◽  
Dewi Wulansari ◽  
Andria Agusta ◽  
Sitarina Widyarini ◽  
Kurniasih
Keyword(s):  
Body Weight ◽  
Food Consumption ◽  
Mus Musculus ◽  
Body Weight Gain ◽  
Drug Candidate ◽  
Male Mice ◽  
Oral Toxicity ◽  
Subchronic Toxicity ◽  
Hydropic Degeneration ◽  
Significant Difference

(+)-2,2’Epicytoskyrin A (EpiA) is a secondary metabolite derived from an endophytic fungus Diaporthe sp. associated with the Gambier plant. EpiA has been reported to possess anticancer, antifungal, and antibacterial activities. In a mouse model, EpiA was also shown to low oral toxicity. Until now, there is no negative effect of EpiA reported yet. This study aimed to study the subchronic toxicity of this component in mice. This study used 60 male mice (Mus musculus) which were divided into four groups of treatments. Parameters that were analyzed were body weight, food consumption, blood chemicals, and histology. The results showed no significant difference in body weight gain and food consumption in all groups. Blood chemicals analysis showed increasing AST and ALT in dosage 12.5 mg/kg BW groups at day 90. Histological changes showed fatty degeneration and hydropic degeneration in the liver and mild inflammation in the kidney. No irreversible toxic effect of this component. So it can be beneficial for plant-based drug discovery. On the other hand, this study provides information about the safety of the development of EpiA as a drug candidate.

scholarly journals Components and acute toxicity of nanoherbal haramonting (Rhodomyrtus tomentosa)

2020 ◽  
Vol 10 (1) ◽  
pp. 139-148
Author(s):  
Putri Cahaya Situmorang ◽  
Syafruddin Ilyas ◽  
Salomo Hutahaean ◽  
Rosidah Rosidah
Keyword(s):  
Lethal Dose ◽  
Preliminary Test ◽  
Average Diameter ◽  
High Energy ◽  
Diameter Distribution ◽  
Chemical Components ◽  
Test Results ◽  
High Concentrations ◽  
Layer Chromatography ◽  
Rhodomyrtus Tomentosa

Introduction: Rhodomyrtus tomentosa (haramonting), a typical plant of North Sumatera, Indonesia, contains important medicinal ingredients. Nano sized drugs have high loading capacities and can be given at high concentrations. This study aimed to determine the components and toxicity of nanoherbal haramonting. It also aimed to determine the effect of nanoherbal haramonting on the histology of the liver, kidneys, lungs, heart and brain. Methods: High-energy milling was performed to produce nanoherbal haramonting. Thin-layer chromatography was utilised to determine the chemical components of the nanoherb. Antioxidant tests were performed by using the 1,1-diphenyl-2-picryhydrazil method. The three-stage of lethal dose 50 (LD50) which comprised the dose orientation test, preliminary test and actual phase test/LD50 determination, and the Thomson–Weil formula was applied to measure the lethal concentration 50 (LC50) of nanoherbal haramonting. Organs were collected for histological investigation after 14 days of the lethality test. Results: Nanoherbal haramonting had an average diameter distribution of 600.1 nm ± 135.8. It contained flavonoids, steroids, glycosides, saponins and tannins. Its LC50 and LD50 values were 2961.535 ppm and 10.4 ± 0.135 mg/kg BW, respectively. The histology of the heart, kidney, lungs, heart and brain were changed and affected by nanoherbal haramonting treatment at each dose level. Conclusion: Nanoherbal haramonting has strong antioxidative activity and small size, can be effectively used as medicine in the future because it contains secondary metabolite compounds that can be developed as drugs. However, it has mild toxicity.

scholarly journals POTENSI SEDATIF-HIPNOTIK DAUN KAYU GALALA (ERYTHRINA LITHOSPERMA) SEBAGAI KANDIDAT OBAT INSOMNIA

2019 ◽  
Vol 20 (2) ◽  
pp. 84-94
Author(s):  
Efraim Samson ◽  
Wahyudi Abdul Hamid Ridwan ◽  
Chomsa Dintasari Umi Baszary
Keyword(s):  
Effective Dose ◽  
Mus Musculus ◽  
Positive Control ◽  
Statistical Test ◽  
Positive Control Group ◽  
Control Group ◽  
Test Results ◽  
Male Mice ◽  
Traditional Medicines ◽  
Hypnotic Effect

The use of drugs from the sedative-hypnotic group to overcome the problem of insomnia began to be abandoned and turned to the use of traditional medicines made from plants. The purpose of this study is to determine the potential of kayu galala leaves (Erythrina lithosperma) as a candidate for medicine of insomnia. A total of 15 male mice (Mus musculus) aged ± 2 months weighing ± 20 grams, divided into control mice (K- and K +), and mice treated with infusion dose of kayu galala leaves, ie P1 0.3 mL/mice; P2 0.5 mL/mice; and P3 0.7 mL/mice. The results showed that kayu galala leaves had a sedative-hypnotic effect. All infusion doses of kayu galala leaves are able to accelerate sedative onset and increase the duration of mice hypnotics, and the most effective dose is 0.7 mL/mice. The statistical test results showed that awarding of kayu galala leaves infusion had more significant effect in accelerating sedative onset and increasing the duration of mice hypnotics, when compared to the positive control group (+). Penggunaan obat-batan dari golongan sedatif-hipnotik untuk mengatasi masalah insomnia mulai ditinggalkan dan beralih pada penggunaan obat-obatan tradisional berbahan tumbuhan. Tujuan dilakukannya penelitian ini, yakni untuk mengetahui potensi sedatif-hipnotik daun kayu galala (Erythrina lithosperma) sebagai kandidat obat insomnia. Sebanyak 15 ekor mencit (Mus musculus) jantan umur ± 2 bulan dengan berat badan ± 20 gram, dibagi ke dalam kelompok mencit kontrol (K- dan K+), serta kelompok mencit perlakuan dosis infusa daun kayu galala, yakni P1 0.3 mL/ekor; P2 0.5 mL/ekor; dan P3 0.7 mL/ekor. Hasil penelitian membuktikan bahwa daun kayu galala memiliki efek sedatif-hipnotik. Seluruh dosis infusa daun kayu galala mampu mempercepat onset sedatif serta meningkatkan durasi hipnotik mencit, dan yang paling efektif yakni dosis 0.7 mL/ekor. Hasil uji statistik juga menunjukkan bahwa pemberian infusa daun kayu galala memiliki pengaruh yang lebih signifikan dalam mempercepat onset sedatif serta meningkatkan durasi hipnotik mencit, bila dibanding kelompok kontrol positif (+).

Sign in / Sign up

Export Citation Format

Share Document