scholarly journals PENGARUH AIR REBUSAN DAUN HARENDONG BULU (Clidemia hirta) TERHADAP KADAR GULA DARAH MENCIT (Mus musculus)

2020 ◽  
Vol 3 (1) ◽  
pp. 7-12
Author(s):  
Okta Malia ◽  
Dian Samitra ◽  
Eka Lokaria
Keyword(s):  
Blood Sugar ◽  
Mus Musculus ◽  
Design Research ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Sugar Levels ◽  
Boiled Water ◽  
One Way Anova

This study aims to determine the effect of the water boiled leaves of Clidemia hirta on blood sugar levels in Swiss Webster mice (Mus musculus). This research using a completely randomized design research design (CRD) with 5 treatment groups namely; Aquades control group (P0), 3 g sucrose treatment (P1), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.13 ml/KgBB (P2), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.26 ml/KgBB (P3), 3 g treatment of sucrose + boiled bristle leaves boiled water at a dose of 0.39 ml/KgBB (P4). Before administration of the extract, mice in the treatment groups P1, P2, P3 & P4 were induced using 3 g of sucrose for 5 days. Then after 5 days for groups P2, P3, and P4 followed by giving boiled water leaves for 7 days. The analysis result of One Way Anova Test shows that Fcount > Ftable (18.86 > 2.51). This shows the boiled water affects the blood sugar levels of male Swiss Webster mice. The concentration of extract at P4 (0.39 ml/KgBB) was more effective in reducing blood sugar levels.

scholarly journals Effect of Boiled Water Tithonia diversifolia A. Gray Leaf Against the Pancreas Histology in Mus musculus L. Induced by Alloxan

BioScience ◽  
2019 ◽  
Vol 3 (1) ◽  
pp. 69
Author(s):  
Yenni Fitri ◽  
Elsa Yuniarti
Keyword(s):  
Blood Sugar ◽  
Tithonia Diversifolia ◽  
Pancreatic Cells ◽  
Randomized Design ◽  
Significant Difference ◽  
Before And After ◽  
Alternative Drug ◽  
Completely Randomized Design ◽  
Sugar Levels ◽  
Boiled Water

Traditional medicine is one of the drugs used by the community to be one of the efforts to treat diseases. One of them is Diabetes Mellitus (DM). DM is a metabolic disease characterized by hyperglycemia which results in an increase in free radicals in the cell. DM treatment is quite expensive so an alternative drug is needed. One of them is Tithonia diversifolia A. Gray. This study used a completely randomized design, consisting of 1 control, 4 treatments (P1: Only alloxan induced), P2: Alloxan 65 mg / kg BB, P3: Metformin 65 mg / kg BB, P4: Boiled leaves of moon leaves 24.6 mg / 10 ml and P4: Moon flower leaves boiled water 49.1 mg / 10 ml. The parameters observed were blood sugar in male mice and the number of cells in the islands of Langerhans before and after being given boiled leaves of moon flowers and metformin. Data were analyzed using ANOVA then continued with DMRT test with a significant difference of 5%. The results showed that administration of boiled kembang bulan leaves and metformin for 7 days could reduce blood sugar levels in mice and accelerate the regeneration of pancreatic cells. The most significant impact is the treatment with the highest dose, namely P4 (Moon leaf leaves boiled water 49.1 mg / 10 ml).

scholarly journals Effect of Nano herbal Haramounting (Rhodomyrtus tomentosa) in Histology of Heart Mus musculus

2019 ◽  
Vol 1 (2) ◽  
pp. 101-106
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Research Use ◽  
Treatment Groups ◽  
High Energy Milling ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the effect of histology of heart Mus musculus  after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the  Completely Randomized Design (CRD). Treatment consists of 6 groups, namely:  Control: CMC 0.5%; T1:100mg/20gBW; T2: 141,42mg/20gBW; T3:200mg/20gBW; T4: 282,82mg/20gBW;  T5:400mg/20gBW. The results of histology hearts that there were significant difference (P<0,05) between  control group and treatment groups but there was not significantly different in weight of hearts (P>0,05).

scholarly journals Description of Kidney Histology of Mus Musculus After Giving Nano Hebal Rhodomyrtus tomentosa (Haramounting)

2019 ◽  
Vol 1 (1) ◽  
pp. 26-33
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Significant Difference ◽  
Proximal Tubular ◽  
Completely Randomized Design ◽  
Paraffin Method ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the description of kidney histology of Mus musculus after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the Completely Randomized Design (CRD), which consists of six groups of CMC 0.5% and Nano- R.tomentosa (Haramonting) at dosage of (100; 141,42; 200; 282,82; 400)mg/20g body weight. The kidneys were made into preparations by using the paraffin method and Hematoxylin Erlich-Eosin staining (HE). The results of histological kidneys showed that there is a significant difference (P<0,05) between control group and treatment groups with damaged cells and increased proximal tubular narrowing in kidneys, but there was not significantly different in weight of kidneys (P>0,05).

scholarly journals PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 169
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Virid Gibson ◽  
Alfiah Hayati
Keyword(s):  
Molecular Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Control Group ◽  
Protein Profiles ◽  
Testosterone Levels ◽  
Randomized Design ◽  
Sds Page ◽  
Elisa Technique ◽  
Completely Randomized Design

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of  15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of  60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>

scholarly journals THE CAPABILITY OF SEDATIVE EFFECT FROM CELERY (Apium graveolens L.) FRACTION TO MALE MICE

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Shinta Kusuma ◽  
Arum Setiawan ◽  
Salni Salni
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Water Fraction ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Traction Test ◽  
Effect Test

Celery fraction research (Apium graveolens L.) was carried out to determine the ability of the sedation effect of celery fraction compared to celery extracts which have been known to have the ability to effect the previous sedation. This study aims to find out which fraction has the best sedation effect. This study was an experimental study with a Completely Randomized Design (CRD) consisting of 5 treatments and 5 replications. Test animals divided into 5 treatment groups namely negative control group (CMC Na 1%), celery extract group 200mg /kg and 3 treatment groups n-hexane fraction, ethyl acetate, and methanol water fraction with a dose of 200 mg /kg . The sedation effect test was carried out using the Traction Test and Fireplace Testmethods. Quantitative data observed were the length of time the mice fell and the length of time the mice went out of the heated tube/glass. The results of the analysis showed that the celery fraction had a better sedation effect than the extract, and the methanol water fraction 200 mg/kg was the most effective fraction in causing sedation effects.

scholarly journals The effect of exposure to liquid air freshener on histological of the bronchial mice (Mus musculus)

2021 ◽  
Vol 6 (02) ◽  
Author(s):  
Warobi Warobi ◽  
Delima Engga Maretha ◽  
Asnilawati Asnilawati ◽  
Mashuri Masri
Keyword(s):  
Mus Musculus ◽  
The Body ◽  
Control Group ◽  
Epithelial Tissue ◽  
Treatment Groups ◽  
Histological Picture ◽  
Randomized Design ◽  
Post Test ◽  
The One ◽  
Post Hoc

Air fresheners are products that contain chemicals aimed at reducing unpleasant odours in confined spaces. The use of synthetic air fresheners turns out to harm health because some of their leased Volatile Organic Compounds are classified as toxic compounds and are carcinogens. Air freshener enters the body through the inhalation process in the respiratory system. Modern air fresheners are available in liquid (aerosol) and gel forms. Air fresheners contain addictive substances and solvents such as 1,4-dichlorobenzene which can affect pulmonary function. The purpose of this study is to know the effect of exposure to liquid air freshener on the histology of bronchi of mice (Mus musculus). This study used 20 male mice (Mus musculus) consisting of 4 treatments with 5 replications. The research design is true experimental in the form of a post-test only control group with Completely Randomized Design (CRD). The post-test was done by observing the histological picture of the mice's bronchi after exposure to liquid air freshener given 3x/day for a period of P1 = 2 weeks, P2 = 4 weeks and P3 = 6 weeks. Quantitative data on bronchial histology was tested using the One Way ANOVA statistical test followed by the Post Hoc Tukey test. The results of the study found changes in the histology of the bronchi, thickening of the epithelial tissue of mice. Analysis of comparative data between the control and treatment groups statistically obtained p = 0.010 <0.05, meaning that there was a significant effect and change in the bronchial tubes exposed to liquid air freshener. Histologically there were differences in histology between the control and treatment groups. It was concluded that liquid air freshener had a significant effect on the histological picture of the mice's bronchi.

scholarly journals Blood profile and gut microbial load of broilers fed siam weed (Chromolaena odorata) leaf meal in their diets

Agricultura ◽  
2017 ◽  
Vol 14 (1-2) ◽  
pp. 17-24 ◽  
Author(s):  
Peter-Damian Chukwunomso Jiwuba ◽  
Ifeanyi Princewill Ogbuewu ◽  
Elisha Dauda ◽  
Caritas Chibuike Azubuike
Keyword(s):  
Immune Status ◽  
Microbial Load ◽  
Control Group ◽  
Pathogenic Microorganisms ◽  
Chromolaena Odorata ◽  
Blood Profile ◽  
Treatment Groups ◽  
Leaf Meal ◽  
Randomized Design ◽  
Completely Randomized Design

AbstractThe responses of 96 day old broilers fed Chromolaena odorata leaf meal (COLM) on blood profile and gut microbial load were studied for 49 days. The birds were randomly assigned to four treatment groups and replicated three times in a completely randomized design. The birds were fed four experimental diets formulated at 0%, 2%, 4% and 6% for the starter phase and 0%, 4%, 8% and 12% of COLM at the finisher phase for T1, T2, T3 and T4 respectively. All the haematological parameters were similar (P>0.05) across the treatment groups except MCH and WBC which were significantly (P< 0.05) higher and better in the treatment groups than the control group. The results on serum chemistry showed significant (P<0.05) difference for total protein, albumin, creatinine and cholesterol across the treatment groups. Cholesterol was significantly (P<0.05) reduced with increasing levels of COLM. Results showed that coliforms and E.coli were consistently higher (P<0.05) among the T1 birds than those fed T2, T3 and T4. It was concluded that, COLM enhanced adequate haematocrit and immune status, hypoglycaemic ability, suppresses the growth of gut pathogenic microorganisms and enhanced the growth of beneficial microorganism in broilers.

scholarly journals Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan

2021 ◽  
Vol 9 (2) ◽  
pp. 79
Author(s):  
Febriyono Anggela Prakoso ◽  
Raden Tatang Santanu Adikara ◽  
Kusnoto Kusnoto ◽  
Sri Hidanah ◽  
Yeni Dhamayanti ◽  
...  
Keyword(s):  
Growth Rate ◽  
Weight Gain ◽  
Research Methods ◽  
Control Group ◽  
Treatment Groups ◽  
Gain Rate ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Bali Cattle

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.

scholarly journals Lowering blood sugar levels with kersen leaf stew (muntingia calabura l) and black onion (alium sativum) extract in mice (mus musculus)

2020 ◽  
Vol 9 (1) ◽  
pp. 15
Author(s):  
Eni Sumarliyah ◽  
Dede Nasrullah ◽  
Sukadiono . ◽  
Desta Pankyano
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Mus Musculus ◽  
Intervention Group ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Onion Extract ◽  
The Difference ◽  
Sugar Levels

Diabetes mellitus (DM) is a metabolic disease characterized by an increase in blood sugar levels. Blood sugar levels in patients with DM are mostly challenging to control, and this will threaten the occurrence of complications. Alternative therapies used are active flavonoids in reducing blood glucose. The purpose of this study was to analyze the decrease in blood sugar levels by administering cherry leaves stew and black onions to mice (mus musculus). The design of this study was the Quasy Experimental design (pretest-posttest with control group design). The sample in this study was 32 mice, 16 in the intervention group, and 16 comparison groups. There are independent variables (Kersen Leaf Stew and Black Onion Extract) and the dependent variable (Blood Glucose Level). The data were collected using an observation sheet, then analyzed using the independent sample T-test and Kolmogorov Smirnov test. The results of this study showed that the pre and post administration of cherry leaf decoction was 36.5 mg/dl, and the difference between pre and post administration of black onion extract was 24.3 mg/dl. While the analysis showed p = value 0.98, it showed no difference in the effectiveness of the two extracts in reducing blood sugar levels in mice. Obtained comparative test results from the two extracts were equally effective in lowering blood sugar in mice. Thus further research needs to be done with more samples.  

scholarly journals The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

2020 ◽  
Vol 13 (8) ◽  
pp. 1594-1598
Author(s):  
Muslim Akmal ◽  
Gholib Gholib ◽  
Mustafa Kamal Nasution ◽  
Sri Wahyuni ◽  
Rinidar Rinidar ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Commercial Chicken ◽  
Male Chicken ◽  
Completely Randomized Design ◽  
Kinase A

Background and Aim: Testis (T) and epididymis (E) are waste from the abattoir that is rarely used. In fact, both organs contain important chemicals needed for spermatogenesis (e.g., hormones, proteins, and other molecules). Therefore, administration of a combination of testis and epididymis (CTE) extracts may activate androgen receptors (AR) and protein kinase A (PKA) molecules that play a prominent role in spermatogenesis. We, therefore, aimed at investigating the influence of the CTE extracts on the concentration of AR and PKA in male chicken. Materials and Methods: This study used a completely randomized design with four treatment groups (K0, K1, K2, and K3) and five replications per group. K0 is a control group that received 1 mL normal saline, whereas K1, K2, and K3 are the test groups that received 1, 2, and 3 mL of CET extracts, respectively. Twenty male chickens (strain: broiler Mb 89), 3 weeks of age, weighing 500-700 g were used. We administered the injections in a 13-day period and on the 14th day; we collected and processed blood samples as serum to measure the AR and PKA concentrations using commercial chicken AR and PKA enzyme-linked immunosorbent assay kits, respectively. We performed analyses by analysis of variance using SPSS 20.0. Results: The AR concentrations in K1, K2, and K3 groups increased by 4.26%, 10.97%, and 28.04%, respectively, compared to the K0 (control group). However, this increase was not significantly different between the groups (p>0.05). Moreover, the PKA concentrations increased by 2.97%, 2.60%, and 4.08% in K1, K2, and K3 groups, respectively, compared to the control group. However, this increase was not significantly different between the groups as well (p>0.05). Conclusion: The CTE extracts tended to increase the AR and PKA concentrations even though it is not significant. Therefore, it needs further study when using the CTE extracts for spermatogenesis in male chicken.

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