scholarly journals Insecticide Activity Extract of Tinospra crispa Mixed With Cow Urine on Spodoptera litura in the Laboratory

2019 ◽  
Vol 6 (2) ◽  
pp. 211-215
Author(s):  
Putri Mustika Sari ◽  
Henny Wahyunita ◽  
Adriansyah Yoesoep ◽  
Lisdayani ◽  
Fitra S. Harahap

Armyworms (Spodoptera litura) become important pests in Indoensia that eat leaves compared to other pests because they are polyfag. S. litura can reduce productivity even crop failure. Therefore, it is necessary to do research on how to control S. litura effectively and environmentally friendly. This study aims to determine the effectiveness of brotowali extract (Tinospra crispa) as a botanical insecticide and obtain the right concentration of fruit extract, young leaves, and old Brotowali leaves to control the armyworm (litura Spodopter). This study used a Non Factorial Completely Randomized Design (RAL) with 6 treatments of various doses of brotowali extract mixed with cow urine and 4 replications. The results of the study showed that the administration of Brotowali + Urine Cattle extract with high doses (30 ml / jar) was able to increase the speed of death of the armyworm.

BIOEDUKASI ◽  
2018 ◽  
pp. 15
Author(s):  
Ahmad Habib Sholahuddin ◽  
Wachju Subchan ◽  
Jekti Prihatin

Grayak caterpillar is insect pests that become pests of various food crops. Grayak caterpillars cause a decrease in crop productivity and even crop failure if they are not controlled. Pest control using chemical pesticides has many adverse effects on the environment. To minimize the occurrence of environmental damage, then it is used pest control using vegetable insecticides. Bintaro Leaf (Cerbera odollam Gaertn.) is potential as a vegetable insecticide. The purpose of this study was to examine the toxicity of granule from Bintaro leaf extract (C. odollam Gaertn.) on armyworms (S. litura Fab.). This research was a laboratory experimental research using Completely Randomized Design (CRD). Serial concentrations used in this study were 0% (control), 0.5%, 1%, 1.5%, 2%, and 2.5%. The results of this study showed that the granules of Bintaro leaf extract (C. odollam Gaertn.) were effective in causing mortality of armyworms (S. litura Fab.) at 1.5% concentration by 60% and included in toxic category. The value of LC50-48 hour granule from Bintaro leaf extract (C. odollam Gaertn) was 1.41%. Keywords: Spodoptera litura Fab. , Granula extract of Cerbera odollam Gaertn., Vegetable Insecticide.


2020 ◽  
Vol 3 (4) ◽  
pp. 113-124
Author(s):  
Anna Farisa ◽  
Muhammad Sayuthi ◽  
Alfian Rusdy

Abstrak Keong Mas adalah salah satu hama utama pada areal pertanaman padi yang tingkat serangannya tergolong cukup tinggi hingga dapat menyebabkan gagal panen. Buah mahkota dewa merupakan tanaman yang berpotensi sebagai pestisida nabati yang diketahui mengandung zat aktif seperti alkaloid, tannin, flavonoid, fenol, saponin, dan minyak atsiri. Tujuan dari penelitian ini adalah untuk mengetahui konsentrasi dan lama perendaman ekstrak dari buah mahkota dewa yang efektif terhadap mortalitas keong mas (Pomacea. canaliculata). Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor. Faktor pertama adalah konsentrasi buah mahkota dewa sebanyak 5 g, 10g dan 15 g dan faktor kedua adalah lama perendaman selama 24 jam, 48 jam, dan 72 jam. Pengamatan meliputi mortalitas keong mas, penghambatan makan dan rata-rata waktu kematian. Hasil pengamatan penggunaan ekstrak buah mahkota dewa sebagai molusisida nabati dengan konsentrasi 15 g/l air dan lama perendaman 72 jam (K3L3) efektif untuk menurunkan daya hidup (motalitas) keong mas hingga mencapai 89,09% dan penghambat makan keong mas tertinggi terdapat pada kombinasi perlakuan K1L2 dengan persentase nilai 47,90%, sedangkan untuk kecepatan waktu kematian keong mas setelah dilakukan pengaplikasian menggunakan ekstrak buah mahkota dewa berkerja efektif pada perlakuan kosentrasi K3 dan lama perendaman efektif pada perlakuan L3.(Concentration Test and Submersion Time of God Crown Extract as Bio Molluscicide On Golden Snail Mortalities Pomacea canaliculata Lamarck)Abstract The golden snail is one of the main pests in rice cultivation area which has a high level of attack so that it can cause crop failure. God’s crown fruit is a plant that has the potential as a bio pesticide which is known containing chemical substances such as alkaloids, tannins, flavonoids, phenols, saponins, and essential oils. The purpose of this research was to determine the concentration and submersion time of extracts from the fruit of god crown which is effective against the mortality of golden snails (Pomacea canaliculata). Research method used was experimental method using Completely Randomized Design method (RAL) with factorial pattern consisting of 2 factors. The first factor was 5 g, 10 g and 15 g of the concentration of the god crown and the second factor were 24 hours, 48 hours and 72 hours of submersion time. The observation included golden snail mortality, feeding inhibition and the average time of mortality. The results of observations of the usage of the god crown fruit extract as bio molluscicide with a concentration of 15 g / l of water and 72 hours of submersion time (K3L3) were effective to reduce the life  (mortality) of snails to reach 89.09% and the highest snail eating inhibitors were found in the combination K1L2 treatment with a percentage value of 47.90%, while for the golden snail average time of mortality after application using fruit extract of god crown worked effectively on the treatment of K3 concentration and the submersion time is effective in L3 treatment.


2016 ◽  
Vol 1 (1) ◽  
pp. 45-52
Author(s):  
Palupi Puspitorini

The aim of this study was to select the best sources of auxin of which it can stimulate the growth of shoots Pineapple plant cuttings. This research is compiled in a completely randomized design (CRD) with 4 treatments and 6 replications. The Data were statistically Analyzed by the DMRT. Level of treatment given proves that no treatment 0%, cow urine concentration of 25%, young coconut water concentration of 25% and Rootone F 100 mg / cuttings. The results showed that cow urine concentrations of 25% and Rootone F 100 mg give the best results in stimulating the growth of shoots pineapple stem cuttings. Experimental results concluded that the effect of this natural hormone were better than the shoots without given hormone.           


EKOLOGIA ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 55-62
Author(s):  
Rangga Eka S. P ◽  
Moerfiah . ◽  
Triastinurmiatiningsih .

The purpose of this study was to determine the activity of the leaf extract of Piper sarmentosum on mortality and damage levels by pest armyworm (Spodoptera litura). This research was conducted at the Laboratory of Biology, Faculty of Mathematics and Natural Sciences, Pakuan University, Bogor. This study uses a completely randomized design (CRD), with four levels of extract concentration, that is 0% as control, 30%, 40% and 50%.  Each treatment was repeated four times. The variables measured were mortality rates, the extent of damage the leaves, and larval growth. A concentration of EDK 50% showed activity in killing the armyworm by 38%. The lowest level of damage is 5%, with an average growth rate to its lowest larvae by 0.8 cm with the provision of treatment EDK 50%, and the higest level of damage is 75% by a concetration of EDK 30% treatments.


2017 ◽  
Vol 17 (2) ◽  
Author(s):  
I Made Indra Agastya ◽  
Aminudin Afandhi ◽  
Luqman Qurata Aini

This research was conducted in vitro in the laboratory. Studies conducted in the Laboratory of Bacteriology Department of Plant Pests and Diseases of the Faculty of Agriculture, University of Brawijaya, on the effectiveness of the bacteria Bacillus cereus and Bacillus megaterium as biological pesticides controlling Spodoptera litura. The objective of this study was to determine the effectiveness of the bacteria Bacillus sp as biological control. Effectiveness pesticide was measured by testing the incubation period and mortality in larvae of S. litura instar 3. This study used a completely randomized design (CRD), conducted observations every 6 hours until the larvae dead. The results showed that the percentage of mortality of S. litura reached 94.66% compared to the control, while the incubation time of the bacteria B. cereus cause disease until 29.84 hours. B. cereus and B. megaterium have the ability to incubate third instar larvae of S. litura up to 29.84 hours and caused the death of larvae up to 94.66%. Keywords: Biological pesticides, Bacillus cereus, Bacillus megaterium, entomopatogen, Spodoptera litura


2019 ◽  
Vol 4 (1) ◽  
pp. 28
Author(s):  
Kartina Kartina ◽  
Shulkipli Shulkipli ◽  
Mardhiana Mardhiana ◽  
Saat Egra

<h1>Spodoptera litura F. is one of the important pests in Horticulture commodities which can cause damage 80% and up to 100%. Karamunting (Melastome malabathricum) is a wild plant that grows in Tarakan City. This plant is known to contain secondary metabolites which are thought to have the potential to control pests. This study aims to determine the potential of Karamunting extract as a botanical insecticide material to control armyworms. This study uses a completely randomized design (CRD) method with 1 treatment factor, namely the concentration of Karamunting leaf extract. Potential extracts as botanical insecticides were tested by dye method using 4 concentrations, namely 0 (control); 500; 1000; 2000; 4000; and 8000 ppm. The results showed that Karamunting leaf extract was able to control armyworm pests. Giving extracts of 8000 ppm (P5) in feed caused the highest decrease in eating activity up to 41,2%, larval mortality up to 85%, and the highest mortality rate after 7 days of observation. Decreased feeding activity and larval mortality were thought to be influenced by the presence of phenol compounds, fatty acids, terpenoids, sterols and alkaloids identified in the extract. Thus, Karamunting leaf extract has the potential to be developed as an active ingredient in botanical insecticides to control armyworm.<strong></strong></h1>


2020 ◽  
Vol 9 (4) ◽  
pp. 458
Author(s):  
Rizky Amalia Rachmawati ◽  
Ni Wayan Wisaniyasa ◽  
I Ketut Suter

This study was conducted to determine the effect of different solvents on the antioxidant activity of gale of the wind extract and to find out the right type of solvent used to extract gale of the wind with the highest antioxidant activity using maceration method. The experimental design used in this study was a completely randomized design (CRD) with a type of solvent as a treatment. The treatment consisting of four levels, namely methanol, ethanol, acetone, isopropanol. All treatments were repeated four times so that they were obtained 16 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect followed by Duncan test. The results showed that the type of solvent treatment had a very significant effect on yield, total phenol, total flavonoids, total tannin, and antioxidant activity (IC50). The results showed that acetone solvent had the best treatment which produced a yield of 22.58%, total phenol content of 188.77 mg GAE/g, total flavonoid content of 247.60 mg QE/g, total tannin content of 297.51mg TAE/g, and antioxidant activity (IC50) of 15.19 ppm.


2019 ◽  
Vol 8 (1) ◽  
pp. 66
Author(s):  
Lina Nindyawati ◽  
Putu Timur Ina ◽  
Anak Agung Istri Sri Wiadnyani

This study aims to determine the effect of steamed potatoes and green bean flour on the characteristics of flakes, and to know the right ratio of steamed potatoes and green bean flour which produced flakes with the best characteristics. The was used in this research completely randomized design with the treatment ratio of steamed potatoes and green bean flour, which consists of 6 levels such as: 35% : 65%, 40% : 60%, 45% : 55%, 50% : 50%, 55% : 45%, 60% : 40%. The treatment was repeated 3 times to obtained 18 units of the experiment. The data were analysed by variance analysis  and if the treatment had an effect on the variable then continued with Duncan test. The result showed steamed potatoes and green bean flour ratio had a significant effect on water content, ash content, fat content, protein content, carbohydrate content, color (hedonic), aroma (hedonic), texture (hedonic and scoring), taste (hedonic) and overall acceptance (hedonic). Ratio of 35% steamed potatoes and 65% green bean flour produced flakes with the best characteristics, with 2.41 % water content, 4.57 % ash content, 19.16% protein content, 10.20% fat content, 63.66% carbohydrate content, color liked, aroma liked, texture crunchy and liked, taste netral and overall acceptance rather liked.


2019 ◽  
Vol 8 (2) ◽  
pp. 197
Author(s):  
Justicia Febi Estermaria Pandiangan ◽  
I Nengah Kencana Putra ◽  
I Desak Putu Kartika Pratiwi

This research was conducted to identify the effect of adding angkak on the color, antioxidant content, change the number of TBA sausage for six days of storage, sensory’s characteristic of mackerel fish sausage, and to identify the right percentage to adds angkak can improve the color, antioxidant content, and change the number of TBA for six days of storage, and sensory’s characterisctic of mackerel fish sausage. The experimental design used was a completely randomized design (CRD). Treatment factor was consist of six concentrations: 0%, 0,5%, 1%, 1,5%, 2%, and 2,5%. Each treatment had three replications so that obtained 18 units of trial. Observations were made on intensity of colour, antioxidant capacity, number of TBA, and organoleptic characteristics (hedonic test and scoring test). Data were analyzed by One-Way Analysis of Variance at level of 1% and 5% and continued by Duncan. The addition of angkak has affected very significant on color intensity, antioxidant capacity, number of TBA, color, taste, and overall acceptance, also significant on texture of mackerel fish sausage. On the others hand, it has not affected on flavor of mackerel fish sausage. The right concentration is obtained from the use of 1 % angkak. It has L*’s score 31,71; a*’s score 21,10; b*’s score 21,13; antioxidant capacity 53,37 mg GAEAC/kg; number of TBA for the 6th day 0,31 g malonaldehide/kg, color rather faded red and rather liked, flavor rather liked, texture liked, taste bitter weak and liked, and overall acceptance liked.


2020 ◽  
Vol 5 (02) ◽  
Author(s):  
Novitasari Priskalia Puteri ◽  
Lusiawati Dewi ◽  
Anggara Mahardika

Efforts to increase protein in Tempe can provide positive values for native Indonesian food. The amount of remaining egg white in the bread industry can be used in making soybean Tempe. Egg white which is rich in albumin has never been used in the manufacture of soybean tempeh by the producer. This research aims to determine the effect of adding raw and steamed egg whites on the protein content of soybean Tempe. This study used a Completely Randomized Design (CRD) with 3 replications. The concentrations of egg whites used in the research included 0%, 2%, 4%, 6%, 8%, and 10%. The research instrument used was the observation sheet for protein content using the Lowry method. The research data were dissolved protein content. The data analysis technique used Two Way ANOVA. The results of the research showed that there was no effect of adding raw and steamed egg whites on soybean Tempe protein content (P > 0.05). However, the addition of raw egg whites can increase the protein content of soybean Tempe, compared to steamed egg whites. The conclusion of this research is the addition of raw and steamed egg whites does not have a significant effect on the protein content of soybean Tempe. The right type of egg white flour to increase the protein content of soybean Tempe is raw egg white flour with a protein content of 15.0 per 100 grams.


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