THE ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS DERIVED FROM LEAVES AND PSEUDOBULBS OF COELOGYNE FLACCIDA LINDL. (ORCHIDACEAE) AGAINST DIFFERENT STAPHYLOCOCCUS AUREUSSTRAINS

The present study was conducted to investigate in vitro antimicrobial activity of ethanolic extracts obtained from leaves and pseudobulbsof Coelogyneflaccida against Staphylococcus aureus subsp. aureus Rosenbach (ATCC®25923™) (mecA negative), Staphylococcus aureus subsp. aureus Rosenbach (ATCC®29213™)(mecA negative, Oxacillin sensitive, weak β-lactamase-producing strain), Staphylococcus aureus NCTC 12493 (mecA positive, Methicillin-resistant, EUCAST QC strain for cefoxitin).The antimicrobial susceptibility testing was done on Muller-Hinton agar by the disc diffusion method (Kirby-Bauer disk diffusion susceptibility test protocol). The leaves and pseudobulbs of C. flaccida plants, cultivated under glasshouse conditions, were sampled at M.M. Gryshko National Botanic Garden (NBG), National Academy of Science of Ukraine. Freshly leaves and pseudobulbs were washed, weighed, crushed, and homogenized in 96% ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and investigated for their antimicrobial activity. The cultivation medium was trypticase soy agar (Oxoid™, UK), supplemented with 10% defibrinated sheep blood. Cultures were grown aerobically for 24 h at 37°C. The cultures were later diluted with a sterile solution of 0.9% normal saline to approximate the density of 0.5 McFarland standard. The McFarland standard was prepared by inoculating colonies of the bacterial test strain in sterile saline and adjusting the cell density to the specified concentration. The examined ethanolic extracts obtained from leaves and pseudobulbs of C. flaccida showed remarkable antibacterial activities against different S. aureusstrains. The observed activity may contribute to the reasons why orchids are used for infectious and inflammatory conditions in ethnomedicine. The research showed that ethanolic extracts of C. flaccida possess the highest antibacterial potency against S. aureus NCTC 12493 strain. These findings led the authors to suggest that these extracts may be used as natural antiseptics and antimicrobial agents in medicine and veterinary practice. Nevertheless, despite the promising results, more research should be carried out to further evaluate the roles of particular compounds, isolated from all parts of orchid plants, attributable to antimicrobial activity.

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IN VITRO SCREENING FOR ANTIMICROBIAL POTENTIAL OF ETHANOLIC LEAF EXTRACTS OF SOME BEGONIA SPECIES AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) STRAIN

The Scientific and Technical Bulletin of the Institute of Animal Science NAAS of Ukraine ◽

10.32900/2312-8402-2020-123-30-38 ◽

2020 ◽

pp. 30-38

Author(s):

Halyna Tkachenko ◽

Natalia Kurhaluk ◽

Lyudmyla Buyun ◽

Vladimir Tomin

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Inhibition Zone ◽

Leaf Extracts ◽

Test Protocol ◽

Methicillin Resistant ◽

Highly Active ◽

Ethanolic Extracts

The antimicrobial activities of ethanolic extracts obtained from the leaves of Begonia solimutata L.B. Sm. & Wassh., Begonia goegoensis N.E.Br., Begonia foliosa Kunth, Begonia × erythrophylla Hérincq, Begonia thiemei C.DC., Begonia peltata Otto & Dietr., Begonia heracleifolia Cham. & Schltdl., Begonia dregei Otto & Dietr., and Begonia mexicana G. Karst. ex Fotsch were assessed against methicillin-resistant Staphylococcus aureus (MRSA) strain. The leaves were brought into the laboratory for antimicrobial studies. Freshly crushed leaves were washed, weighed, and homogenized in 96% ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and investigated for their antimicrobial activity. The testing of the antibacterial activity of the plant extracts was carried out in vitro by the Kirby-Bauer disc diffusion susceptibility test protocol. The most effective plants among species screened against S. aureus NCTC 12493 growth were B. peltata, B. thiemei, B. foliosa, Begonia × erythrophylla, and B. solimutata being highly active with the ethanolic extracts (diameters of inhibition zone ranged from 12.5 to 21 mm). The ethanolic extracts from nine Begonia plant species were evaluated for their antimicrobial activity against methicillin-resistant S. aureus (MRSA) strain. Extracts from all test Begonia plants caused a remarkable reduction in bacterial growth, measured as an inhibition zone diameters. The diameter of the inhibition zone for B. solimutata was (16.4±1.1) mm, for B. goegoensis – (14.2±1.0) mm, for B. foliosa – (14.9±1.2) mm, for Begonia × erythrophylla – (16.9±0.9) mm, for B. thiemei – (16.8±1.3) mm, for B. peltata – (18.1±0.9) mm, for B. heracleifolia – (15.3±0.9) mm, for B. dregei – (14.7±1.1) mm, and for B. mexicana – (13.8±0.9) mm/ The highly active antimicrobial effects of extracts obtained from B. peltata and B. thiemei noted against S. aureus NCTC 12493 growth is worthy of highlighting. Our studies indicated that Begonia plants are worthy of further investigation as a potential phytotherapeutic agent for treating infections caused by S. aureus., as well as for the development of innovative feed and preventative products in animal husbandry.

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In vitro antimicrobial activity of ethanolic extracts obtained from Ficus spp. leaves against the fish pathogen Aeromonas hydrophila

Archives of Polish Fisheries ◽

10.1515/aopf-2016-0019 ◽

2016 ◽

Vol 24 (4) ◽

pp. 219-230 ◽

Cited By ~ 7

Author(s):

Halyna Tkachenko ◽

Lyudmyla Buyun ◽

Elżbieta Terech-Majewska ◽

Zbigniew Osadowski

Keyword(s):

Antimicrobial Activity ◽

Aeromonas Hydrophila ◽

Bacterial Infections ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Diffusion Method ◽

Moderate Activity ◽

In Vitro Antimicrobial Activity ◽

Ethanolic Extracts

Abstract The main goal of this study was to determine in vitro antimicrobial activity of ethanolic extracts obtained from the leaves of various Ficus species against Aeromonas hydrophila isolated locally from infected rainbow trout (Oncorhynchus mykiss Walbaum) with the aim of providing scientific rationale for the use of the plant in the treatment of bacterial infections induced by Aeromonas spp. in fish. Antimicrobial susceptibility testing was done on Muller-Hinton agar with the disc diffusion method. In the present study, most ethanolic extracts proved effective against the A. hydrophila tested, with 10-12 mm inhibition zones observed. A. hydrophila demonstrated the highest susceptibility to F. pumila. Among various species of Ficus with moderate activity against A. hydrophila, the highest antibacterial activities were noted for F. benghalensis, F. benjamina, F. deltoidea, F. hispida, and F. lyrata. Thus, Ficus can be used as a natural antiseptic and antimicrobial agent in veterinary practice. Further investigations need to be conducted to isolate and identify the bioactive compounds that can then be subjected to detailed pharmacological studies and the development of clinical applications. The alarming rate of increasing resistance in bacterial pathogens in aquaculture environments means that medicinal plants with antibacterial properties are very important as natural resources of new active compounds.

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An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽

10.3390/ma13194383 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4383

Author(s):

Barbara Lapinska ◽

Aleksandra Szram ◽

Beata Zarzycka ◽

Janina Grzegorczyk ◽

Louis Hardan ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antifungal Activity ◽

Antimicrobial Agents ◽

Resin Composite ◽

Antimicrobial Properties ◽

In Vitro Study ◽

Diffusion Method ◽

Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

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SYNTHESIS AND STRUCTURAL ELUCIDATION OF AMINO ACETYLENIC AND THIOCARBAMATES DERIVATIVES FOR 2-MERCAPTOBENZOTHIAZOLE AS ANTIMICROBIAL AGENTS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15760 ◽

2017 ◽

Vol 9 (2) ◽

pp. 192

Author(s):

Aseel Alsarahni ◽

Zuhair Muhi Eldeen ◽

Elham Al-kaissi ◽

Ibrahim Al- Adham ◽

Najah Al-muhtaseb

Keyword(s):

Antimicrobial Activity ◽

Elemental Analysis ◽

Antimicrobial Agents ◽

Diffusion Method ◽

Benzothiazole Derivatives ◽

H Nmr ◽

Ft Ir ◽

Potential Antimicrobial Activity ◽

C Nmr

Objective: To design and synthesize amino acetylenic and thiocarbonate of 2-mercapto-1,3-benthiazoles as potential antimicrobial agents.Methods: A new series of 2-{[4-(t-amino-1-yl) but-2-yn-1-yl] sulfanyl}-1,3-benzothiazole derivatives (AZ1-AZ6), and S-1,3-benzothiazol-2-yl-O-alkyl carbonothioate derivatives were synthesised, with the aim that the target compounds show new and potential antimicrobial activity. The elemental analysis was indicated by the EuroEA elemental analyzer, and biological characterization was via IR, 1H-NMR, [13]C-NMR, DSC were determined with the aid of Bruker FT-IR and Varian 300 MHz spectrometer using DMSO-d6 as a solvent. In vitro antimicrobial activity, evaluation was done for the synthesised compounds, by agar diffusion method and broth dilution test. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined. Results: The IR, 1H-NMR, 13C-NMR, DSC and elemental analysis were consistent with the assigned structures. Compound of 2-{[4-(4-methylpiperazin-1-yl)but-2-yn-1-yl] sulfanyl}-1,3-benzothiazole (AZ1), 2-{[4-(2-methylpiperidin-1-yl)but-2-yn-1-yl]sulfanyl}-1,3-benzothiazole (AZ2), 2-{[4-(piperidin-1-yl) but-2-yn-1-yl]sulfanyl}-1, 3-benzothiazole (AZ6), S-1,3-benzothiazol-2-yl-O-ethyl carbonothioate (AZ7), and S-1,3-benzothiazol-2-yl-O-(2-methylpropyl) carbonothioate (AZ9) showed the highest antimicrobial activity against Pseudomonas aeruginosa (P. aeruginosa), AZ-9 demonstrated the highest antifungal activity against Candida albicans (C. albicans), with MIC of 31.25 µg/ml.Conclusion: These promising results promoted our interest to investigate other structural analogues for their antimicrobial activity further.

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Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)

BioMed Research International ◽

10.1155/2020/4150678 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Ali Al Bshabshe ◽

Martin R. P. Joseph ◽

Amgad A. Awad El-Gied ◽

Abdalla N. Fadul ◽

Harish C. Chandramoorthy ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Clinical Relevance ◽

Mangifera Indica ◽

Diffusion Method ◽

Drug Candidate ◽

Methicillin Resistant ◽

Mango Kernel ◽

Ethanolic Extracts ◽

Mrsa Strains

Methicillin-resistant Staphylococcus aureus (MRSA) is known for serious health problems. Testing new inexpensive natural products such as mango kernel (Mangifera indica L., Anacardiaceae) may provide alternative and economically viable anti-MRSA drugs. In the current study, we screened clinical isolates from Aseer Central Hospital, Saudi Arabia, during 2012–2017 for MRSA and tested an ethanolic extract of mango kernel for anti-MRSA activity. Brief confirmation of MRSA was performed by the Vitek 2 system, while antibiotic sensitivity of strains was tested for their clinical relevance. The In vitro disc diffusion method was used to test the anti-MRSA activity of the ethanolic mango kernel extract. The antimicrobial activity of mango kernel was compared to that of standard drugs (oxacillin and vancomycin). Of the identified 132 S. aureus strains, 42 (31.8%) were found to be MRSA and their prevalence showed a clear increase during the last two years (2016-2017; p<0.001). MRSA strains showed 100% sensitivity to vancomycin, teicoplanin, linezolid, tetracycline, daptomycin, tigecycline, and tobramycin and 100% resistance to ampicillin and 98% to penicillin. The ethanolic extracts of mango kernel were found active against both S. aureus and the MRSA strains. Inhibitory activities (mean ± SE) were achieved at concentrations of 50 mg/mL (20.77 ± 0.61), 5 mg/mL (16.18 ± 0.34), and 0.5 mg/mL (8.39 ± 0.33) exceeding that of vancomycin (p=0.0162). MRSA strains were sensitive to mango kernel extracts when compared to vancomycin. Therefore, ethanolic extracts of mango kernel can be escalated to animal model studies as a promising leading anti-MRSA drug candidate and can be an economic alternative to high-priced synthetic antibiotics.

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Structure-activity relationships of 3-substituted-5,5- diphenylhydantoins as potential antiproliferative and antimicrobial agents

Journal of the Serbian Chemical Society ◽

10.2298/jsc110314143t ◽

2011 ◽

Vol 76 (12) ◽

pp. 1597-1606 ◽

Cited By ~ 9

Author(s):

Nemanja Trisovic ◽

Bojan Bozic ◽

Ana Obradovic ◽

Olgica Stefanovic ◽

Snezana Markovic ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Agents ◽

Human Colon ◽

Antibacterial Activities ◽

E Coli ◽

Structure Activity Relationships ◽

Structure Activity ◽

Almost All

A series of twelve 3-substituted-5,5-diphenylhydantoins was synthesized, including some whose anticonvulsant activities have already been reported in the literature. Their antiproliferative activities against HCT-116 human colon carcinoma cells were evaluated to determine structure-activity relationships. Almost all of the compounds exhibited statistically significant antiproliferative effects at a concentration of 100 ?M, while the derivative bearing a benzyl group was active even at lower concentrations. Moreover, their in vitro antibacterial activities against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and clinical isolates of Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus were evaluated. Only the 3-iso-propyl and 3-benzyl derivatives showed weak antibacterial activities against the Gram-positive bacterium E. faecalis and the Gram-negative bacteria E. coli ATCC 25922 and E. coli.

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AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN HOLE-PLATE DIFFUSION METHOD

Journal of Biological Researches ◽

10.23869/bphjbr.9.1.200310 ◽

2003 ◽

Vol 9 (1) ◽

pp. 49-51

Author(s):

Farida Lanawati Darsono ◽

Stephanie Devi Artemisia

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Traditional Medicine ◽

Tween 80 ◽

Antibacterial Activities ◽

Psidium Guajava ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Gram Positive Bacteria ◽

Activity Test

A study has been performed on the antimicrobial activities of jambu biji (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of jambu biji are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of jambu biji for each cultivar were obtained by reflux with ethanol 96 percent. The concentrations of the extracts applied to the holes were 10 percent, 20 percent, and 30 percent w/v, the extracts were reconstituted with tween 80 and ethanol 96 percent. Based on the result of the study, it can be concluded that the extract of jambu biji from each cultivar with the concentration of 10 percent, 20 percent, and 30 percent w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3x3 and furthery tested for significancy (a = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.

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Screening of antimicrobial activity of aqueous and ethanolic extracts of some medicinal plants from Cameroon and assessment of their synergy with common antibiotics against multidrug-resistant uropathogenic bacteria.

10.1101/2021.09.10.459100 ◽

2021 ◽

Author(s):

Mbarga Manga Joseph Arsene ◽

Podoprigora Irina Viktorovna ◽

Anytoulou Kitio Linda Davares ◽

Mouafo Hippolyte Tene ◽

Irma Aurelia Monique Manga ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Klebsiella Oxytoca ◽

Diffusion Method ◽

Distilled Water ◽

Strong Activity ◽

E Coli ◽

Ethanolic Extracts ◽

Uropathogenic Bacteria

Background and aim: The Cameroonian flora abounds in plants with multidimensional therapeutic virtues which can play an important role in the fight against resistance to antibiotics and the search for new antimicrobials. This study aimed to screen the antimicrobial potential of aqueous and ethanolic extracts of thirteen (13) samples (Bark, leaf, seed) of eight (8) plants from Cameroon against 3 reference pathogens and to evaluate their synergy with conventional antibiotics against eleven (11) multiresistant uropathogenic (MRU) bacteria. Method: Bioactive compounds were extracted from leaves of Leucanthemum vulgare, Cymbopogon citratus (DC.) Stapf, Moringa oleifera Lam and Vernonia amygdalina Delile; barks of Cinchona officinalis and Enantia chlorantha Oliv; barks and seeds of Garcinia lucida Vesque and leaves and seeds of Azadirachta indica (Neem) using distilled water and ethanol as solvents. The extracts were tested against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 and Candida albicans 10231 using the well diffusion method and the microdilution method. The synergistic effect was assessed (using disc diffusion method and the checkerboard method) against MRU bacteria namely Achromobacter xylosoxidans 4892, Citrobacter freundii 426, Enterococcus avium 1669, Escherichia coli 1449, Klebsiella oxytoca 3003, Kocuria rizophilia. 1542, Moraxella catarrhalis 4222, Morganella morganii 1543, Pseudomonas aeruginosa 3057, Staphylococcus aureus 1449 and Streptococcus agalactiae 3984. Results: We found that distilled water extracted a more important mass of phytochemical compounds (7.9-21.2%) compared to ethanol (5.8-12.4%). Except C. officinalis and G. lucida leaves, the rest of extracts were active with inhibition diameters (ID) ranging from 5 to 36 mm. Both ethanolic (EE) and aqueous extract (AE) of E. chloranta bark (ECB) were the most active against all pathogens with the mean ID of 17 and 36 mm vs S. aureus ATCC 6538, 23 and 14 mm vs E. coli ATCC 25922 and 36 and 19 mm vs C. albicans ATCC 10231. Only the EE of E. chloranta bark (ECB) had a strong activity against all the microorganisms tested (MIC <2 mg / ml); L. vulgare leaves (LVL) and G. lucida seed (GLS) had moderate (average MIC of 8 mg/ml) while all other extracts showed very weak antimicrobial activity. In addition, the fractional inhibitory concentration (FIC) ranged from 0.125 to 0.750. No antagonism (FIC> 4) or indifference (1≤ FIC≤4) was noted between the extracts and the antibiotics, but the best synergies were found with ECB which well-modulated Kanamycin (FIC = 0.125 against S. aureus and 0.250 against E. coli), nitrofurantoin (FIC = 0.250 against S. aureus and 0.188 against E. coli) and ampicillin (FIC = 0.125 against E. coli). Similarly, compared to other extracts, ECB, LVL and GLS also well-modulated ampicillin, ceftazidime, tetracycline, nitrofurantoin, and trimethoprim against all the above-mentioned resistant uropathogenic bacteria with important increase in fold area (IFA). Conclusion: This study show that E. chlorantha bark, L. vulgare leaves G. lucida seed, have good antimicrobial activity against both bacteria (Gram positive and Gram negative) and fungi (C. albicans); and should be more investigated for their possible use to the fight against MDR and MRU microorganisms.

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Antibacterial of Cinnamon Bark (Cinnamomum burmannii) Essential Oil Against Methicillin-Resistant Staphylococcus aureus

Jurnal Medik Veteriner ◽

10.20473/jmv.vol4.iss1.2021.56-62 ◽

2021 ◽

Vol 4 (1) ◽

pp. 56

Author(s):

Shinta Levea Ni'matul Fadlilah ◽

Mustofa Helmi Effendi ◽

Wiwiek Tyasningsih ◽

Lucia Tri Suwanti ◽

Jola Rahmahani ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Essential Oil ◽

Methicillin Resistant Staphylococcus Aureus ◽

Antibacterial Activities ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Methicillin Resistant ◽

Cinnamon Bark ◽

Cinnamomum Burmannii

This study aimed to determine the in vitro antibacterial activities of essential oil from cinnamon bark (Cinnamomum burmannii) on Methicillin-resistant Staphylococcus aureus (MRSA) isolated from raw milk. Essential oil from cinnamon bark obtained from the steam distillation method and essential oil was made in a series dilution with a concentration of 1%, 2%, 4%, and 8%. The antibacterial activities were tested using the disk diffusion method. Results showed from five isolates of MRSA, one isolate was sensitive of essential oil with a concentration 2% and all of the isolate was sensitive with a concentration 4% and 8%. The antibacterial activity showed by inhibition zones on MRSA. The results suggest that the activity of the essential oils of cinnamon bark has an antibacterial effect on MRSA and it is developed as phytopharmaca.

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Aqueous Extract of Wild Cyclamen Tubers (Cyclamen Purpurascens L.) - A Potential Source of Natural Antioxidants and Antimicrobial Agents

Quality of Life (Banja Luka) - APEIRON ◽

10.7251/qol1801013s ◽

2018 ◽

Vol 16 (1-2) ◽

Author(s):

Ljiljana P. Stanojević ◽

Milorad D. Cakić ◽

Jelena S. Stanojević ◽

Dragan J. Cvetković ◽

Bojana R. Danilović

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Aqueous Extract ◽

Antimicrobial Agents ◽

Natural Antioxidants ◽

Diffusion Method ◽

Total Phenolic ◽

Dry Extract ◽

Potential Source ◽

Total Flavonoids Content

Wild cyclamen tubers (Cyclamen purpurascens Mill.) (mountain Kukavica, Southeast Serbia) were used as material for extraction in this study. Aqueous extract was obtained by reflux extraction on boiling temperature with hydromodulus 1:20 m/v during 180 minutes. The total phenolic content was determined spectrophotometrically by the method of Folin-Ciocalteu, and the total flavonoids content by method with AlCl3. The antioxidant activity of extract was investigated spectrophotometrically by DPPH and ABTS test. Disc-diffusion method was used for antimicrobial activity investigation on the following pathogenic microorganisms: Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa and Klebsiella pneumoniae. The content of total phenols was 8.27 mg GAE/g dry extract while the total flavonoid content was 11.51 mg RE/g dry extract. The extract concentrations required to neutralize 50% of the initial concentration of DPPH radicals (EC50) after 20 minutes incubation and immediately after adding DPPH radical solution were 0.413 and 2.0 mg/ml, respectively, while concentrations of extract required to neutralize 50% of the initial ABTS radicals concentration is 0.743 mg/ml. The extract showed the highest antimicrobial activity on bacteria Staphylococcus aureus. The presented results indicate that cyclamen tubers extract is a potential source of natural antioxidants and antimicrobial agents.

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