scholarly journals The Effect of Long Shelf Life of Duroc Pig Male Semen Diluted Using Tris-Egg Yolk-Young Coconut Water on The Value of Viability, Abnormality and pH

2021 ◽  
Vol 3 (2) ◽  
pp. 108-120
Author(s):  
Fransiska Luruk Berek ◽  
Agustinus Agung Dethan ◽  
Paulus Klau Tahuk
Keyword(s):  
Shelf Life ◽  
Egg Yolk ◽  
Mean Value ◽  
Coconut Water ◽  
Average Value ◽  
Randomized Design ◽  
Boar Semen ◽  
The Mean ◽  
Completely Randomized Design ◽  
Fresh Semen

This study aims to determine the effect of shelf life of duroc boar semen which was diluted using tris-egg yolk-coconut water thinner on the value of viability, abnormality, and acidity (pH). The semen used is in the form of fresh semen from 2-year-old duroc pigs. Semen was collected by a manual method using artificial broodstock. This research was conducted in November 2020 using an experimental method according to the Completely Randomized Design (CRD) procedure with 4 treatments and 4 replications so that there were 16 experimental units. Each treatment is P0: storage of semen for 0 hours, P1: storage of semen for 24 hours, P2: storage of semen for 36 hours, P3: storage of semen for 48 hours. The results showed that the use of tris-egg yolk-coconut water diluent had a significant effect (P <0.05) on viability, abnormality, and degree of acidity (pH). The mean value of spermatozoa viability was P0: 96%, P1: 93.75%, P2: 84.5%, and treatment P3: 79.5%. The mean value of spermatozoa abnormality in treatment P0: 5.25%, P1: 6.5%, P2: 11.25%, and treatment P3: 14.75%. The average value of the degree of acidity (pH) of semen P0: 8.3 P1: 7.2; P2 7,3 and P3 treatment: 7,25. It can be concluded that the dilution of semen using tris-egg yolk and coconut water can maintain the value of viability, abnormalities of spermatozoa, and degree of acidity (pH).  

scholarly journals EFEK PENAMBAHAN ENZIM (Amylase, Protease, Xylanase) DALAM PAKAN TERHADAP BERAT TELUR DAN DIAMETER KUNING TELUR PADA ITIK CAMPURAN

2021 ◽  
Vol 11 (1) ◽  
pp. 39-47
Author(s):  
Bagus Uda Palgunadi ◽  
Ratna Widyawati ◽  
Dian Ayu Kartika Sari ◽  
Halimah Tusadiah
Keyword(s):  
Egg Yolk ◽  
Egg Weight ◽  
Experimental Animals ◽  
Anova Test ◽  
Average Value ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

The purpose of this research aims to determine the effect of adding enzymes (amylase, protease, xylanase) in feed on egg weight and egg yolk diameter in mixed ducks. The experimental animals used were 24 laying ducks. The design used was a completely randomized design (CRD) with 4 treatments and 6 replications. The four treatments were P0 (Control), P1 (6 ducks with Avizym1502®5gram / kg), P2 (6 ducks with Avizym1502® 10gram / kg), and P3 (6 ducks given Avizym1502). ® 15gram / kg). This treatment is carried out once a day for 9 weeks. Before the treatment, the ducks were adapted for a week. After that, the initial egg weight and yolk diameter were measured at week 0, week 6, and week 9. Data obtained from the results of weighing eggs found an average value of P0 (59.1667 ± 3.9200a) g, P1 5gram / kg (56.1667 ± 2.9268a) g, P2 10gram / kg (60.1667 ± 4.9966a) g, and P3 15gram / kg (67.6667 ± 2.7325ᵇ) g. The results of data from examining the diameter of the egg yolk found an average value of P0 (45.03 ± 1.2956a) mm, P1 5gram / kg (44.35 ± 0.7969a) mm, P2 10gram / kg (45.75 ± 1.5694a) mm, and P3 15gram / kg (48.83 ± 0.7394ᵇ) mm. The data obtained were then analyzed using the ANOVA test which statistically showed that there was a significant difference (P <0.05) between egg weight and yolk diameter in mixed ducks treated with enzymes (amylase, protease, xylanase) in the feed.

scholarly journals Motility, Viability, Spermatozoa Abnormality, and pH of Bali Cattle Semen in Another-Yellow Water Driller Stored in a Different Time

2021 ◽  
Vol 3 (2) ◽  
pp. 76-90
Author(s):  
Fransiskus X. Manehat ◽  
Agustinus Agung Dethan ◽  
Paulus Klau Tahuk
Keyword(s):  
Positive Impact ◽  
Egg Yolk ◽  
Sugarcane Juice ◽  
Spermatozoa Motility ◽  
Average Value ◽  
Cane Juice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Bali Cattle

This study aims to determine the quality of Bali cattle spermatozoa diluted using sugarcane juice-egg yolk. This research was carried out in the stables and Laboratory of the Faculty of Agriculture, University of Timor in August-September 2020. Semen was collected from an adult male bali cattle, aged ± 4.5 years in healthy condition. The method used in this study is an experimental method using a completely randomized design consisting of 4 treatments and 5 replications. Each treatment was T1: 0.075 ml semen + 0.3 ml of egg yolk sugarcane juice diluent and stored for 24 hours, T2: semen 0.075 ml + 0.3 ml of diluent for egg yolk cane juice and stored for 48 hours, T3: semen 0.075 ml + 0.3 ml of diluent for egg yolk sugarcane juice and stored for 72 hours, T4: semen 0.075 ml + 0 ,3 ml of egg yolk sugarcane juice diluent and stored for 96 hours. The variables measured were individual motility, viability, spermatozoa abnormalities, and semen pH. The results showed that the T4 treatment on a shelf life of 96 hours still showed a good average value with individual spermatozoa motility values of 45%, spermatozoa viability of 77.3%, spermatozoa abnormalities of 12.5% and semen pH 6.6. It can be concluded that the use of cane juice-egg yolk diluent has a positive impact because it is able to maintain the viability of spermatozoa for 96 hours.    

scholarly journals Pengaruh penggunaan tepung emping melinjo (gnetum gnemon) dalam pakan terhadap ph, kadar air, dan daya susut daging ayam kampung

2018 ◽  
Vol 6 (2) ◽  
pp. 16-23
Author(s):  
Yopianus Anamila ◽  
Dyah Lestari Yulianti ◽  
Aju Tjatur Nugroho Krisnaningsih
Keyword(s):  
Water Content ◽  
Ph Value ◽  
Mean Value ◽  
Chicken Meat ◽  
Experimental Unit ◽  
Average Value ◽  
Randomized Design ◽  
Significant Difference ◽  
Gnetum Gnemon ◽  
The Mean

ABSTRAK                    Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan tepung emping melinjo (Gnetum gnemon) pada pakan terhadap pH, kadar air, dan penyusutan daging ayam. Dalam percobaan dengan rancangan acak lengkap 4 perlakuan, 4 ulangan, satu unit eksperimen yang terdiri dari 5 ayam ayam yang dibagi secara acak. Keempat ulangan tersebut konsisten dengan empat perlakuan dari penelitian ini, masing-masing mengandung P0, P1, P2, dan P3 adalah 0% (kontrol), 5%, 10%, dan 15%. Melinjo emping flour (Gnetum gnemon) adalah sebagai pakan tambahan yang didapat dari pasaran. Variabel penelitian adalah: pH, kadar air, dan konsumsi daging ayam. Hasil penelitian menunjukkan bahwa kadar keripik leleh (Gnetum gnemon) tidak berpengaruh terhadap pH daging (P> 0,05) sedangkan pada kadar air dan penyusutan Daging ayam menunjukkan perbedaan pengaruh yang sangat signifikan (P <0,01). Nilai pH rata-rata pada perlakuan P0, P1, P2, dan P3 adalah 5,9; 5.9; 6.0 dan 5.9. Nilai rata-rata kadar air dalam perlakuan P0, P1, P2, dan P3 adalah 79,30; 84,90; 83.30, dan 83.40. Nilai rata-rata penyusutan daging ayam dalam perlakuan P0, P1, P2, dan P3 adalah 2,7; 4.2; 4,7 dan 4,7. Disimpulkan bahwa penambahan pati emping melinjo (Gnetum gnemon) berpengaruh pada kadar air dan penyusutan daging ayam. Namun, penggunaan tepung emping melinjo (Gnetum gnemon) tidak berkaryh pada pH daging ayam.    ABSTRACT                     This study aims to determine the effect of the use of emping melinjo flour (Gnetum gnemon) in a feed to pH, moisture content, and shrinkage of chicken meat. In an experiment with a completely randomized design of 4 treatments, 4 replications, one experimental unit consisting of 5 randomly divided chicken chickens. The four replications were consistent with four treatments from the study, each containing P0, P1, P2, and P3 were 0% (control), 5%, 10%, and 15%. Melinjo emping flour (Gnetum gnemon) is as an additional feed obtained from the market. The research variables were: pH, Water content, and Chicken Meat Meat Consumption. The results showed that the level of using melting chips (Gnetum gnemon) did not give effect to meat pH (P> 0,05) while in water content and shrinkage Chicken meat showed a very significant difference of influence (P <0.01). The mean pH value at treatment P0, P1, P2, and P3, was 5.9; 5.9; 6.0 and 5.9. The average value of water content in the treatment of P0, P1, P2, and P3 was 79.30; 84.90; 83.30, and 83.40. The mean value of shrinkage of chicken meat in treatment P0, P1, P2, and P3 was 2.7; 4.2; 4.7 and 4.7. It was concluded that the addition of emping melinjo starch (Gnetum gnemon) had an effect on water content and shrinkage of chicken meat. However, the use of emping melinjo flour (Gnetum gnemon) is not significant at the pH of chicken meat.

scholarly journals Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

2021 ◽  
Author(s):  
Ștefan Ciornei ◽  
Dan Drugociu ◽  
Liliana Margareta Ciornei ◽  
Mihai Mareș ◽  
Roșca Petru
Keyword(s):  
Escherichia Coli ◽  
Shelf Life ◽  
Candida Parapsilosis ◽  
Fungal Species ◽  
Average Value ◽  
Microbiological Profile ◽  
Fungal Load ◽  
Pig Farms ◽  
Boar Semen ◽  
Fresh Semen

The aim of the study is to establish the complete microbiological profile of boar semen and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pigs. More than one hundred semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by determining the degree of con-tamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149x103CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 x103CFU/mL. 23 bacterial and fungal species were isolated, the most common being Candida parapsilosis/sake (92%), Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm (by 49.85% in bacteria and by 9.67% in fungi). The load in bacteria and filamentous fungi can be con-trollable, but not in levuras fungi. In some Fluconazole-added extenders (12.5mg%), ensures the solution of this problem, and even increase in sperm progressivity (8.39%) for at least a 12-hour shelf life after dilution. The validation of the experiment was done by obtaining the sow fertility rate after AI.

scholarly journals Efektivitas Gracilaria gigas sebagai Biofilter Logam Berat Tembaga (Cu) pada Media dengan Salinitas yang Berbeda

2019 ◽  
Vol 8 (2) ◽  
pp. 87
Author(s):  
Yovita Noor Hidayah ◽  
Endang Supriyantini ◽  
Suryono Suryono
Keyword(s):  
Heavy Metal ◽  
Aquatic Ecosystems ◽  
Mean Value ◽  
Absorption Capacity ◽  
Average Value ◽  
Randomized Design ◽  
Negative Effect ◽  
Experimental Laboratory ◽  
Wet Weight ◽  
Completely Randomized Design

Limbah hasil kegiatan industri dan pertanian memberikan efek negatif pada ekosistem perairan laut, seperti logam berat Cu. mempunyai potensi toksisitas yang tinggi terhadap lingkungan perairan. Dinding sel Gracilaria gigas memiliki polisakarida yang mampu menyerap logam Cu. Tujuan penelitian ini untuk mengetahui kemampuan daya absorpsi Gracilaria gigas terhadap logam Cu dalam  media pemeliharaan dengan salinitas berbeda. . Metode yang digunakan adalah eksperimental  laboratoris, menggunakan rancangan acak lengkap (RAL) dan 3 pengulangan setiap perlakuan. Perlakuan menggunakan 5 tingkat salinitas  yaitu 10, 20, 25, 30, dan 40 ‰ dengan  pemeliharaan selama 28 hari. Hasil penelitian menunjukkan bahwa perlakuan salinitas yang berbeda tidak berpengaruh terhadap penyerapan logam berat Cu oleh Gracilaria gigas (p>0,05). Konsentrasi logam berat Cu dalam media pemeliharaan tertinggi pada perlakuan E (40 ‰) dengan rerata nilai 0,11; 0,10; 0,13; dan 0,17 ppm.  Kapasitas penyerapan logam Cu oleh Gracilaria gigas tertinggi terdapat pada hari ke 7 perlakuan A (25 ‰) dengan nilai rerata 0,007 mg/g dan efektivitas penyerapan sebesar 82,77 %. Berat basah G. gigas tertinggi terjadi pada hari ke 7 dengan rerata nilai A (25 ‰) 227,11 g; B (10 ‰) 240,55 g; C (20 ‰) 236,44 g; D (30 ‰) 221,44 g; dan E (40 ‰) 203,77 g. Berat mutlak dan laju pertumbuhan spesifik (SGR)  tertinggi terdapat pada perlakuan E (40 ‰), yaitu -34,22 g dan -0,70% per hari.  Semakin tinggi salinitas, maka pertumbuhan G. gigas akan semakin rendah sehingga akan mempengaruhi daya absorpsi logam Cu. Waste from industrial and agricultural activities has a negative effect on marine aquatic ecosystems, such as heavy metals Cu has a high potential for toxicity to the aquatic environment. The cell wall of G. gigas has polysaccharides which are able to absorb Cu metal. The purpose of this study was to determine the ability of G. gigas absorption to Cu metal in maintenance media with different salinity. . The method used is an experimental laboratory, using a completely randomized design (CRD) and 3 repetitions of each treatment. The treatment uses 5 levels of salinity namely 10, 20, 25, 30, and 40 ‰ with maintenance for 28 days. The results showed that different salinity treatments did not affect the absorption of heavy metal Cu by G.gigas (p> 0.05). The highest concentration of heavy metal Cu in the maintenance media at treatment E (40 ‰) with a mean value of 0.11; 0.10; 0.13; and 0.17 ppm. The highest absorption capacity of Cu by G. gigas was on the 7th day of treatment A (25 ‰) with an average value of 0.007 mg / g and the effectiveness of absorption of 82.77%. The highest wet weight of G. gigas occurred on the 7th day with an average value of A (25 227) 227.11 g; B (10 ‰) 240.55 g; C (20 ‰) 236.44 g; D (30 ‰) 221.44 g; and E (40 203) 203.77 g. The highest absolute weight and specific growth rate (SGR) was found in treatment E (40 ‰), which was -34.22 g and -0.70% per day. The higher the salinity, the growth of  G. gigas will be lower so that it will affect the absorption of Cu metal.

scholarly journals Daya Hidup Spermatozoa Epididimis Sapi Persilangan yang Dipreservasi dengan Air Kelapa Muda pada Suhu 5oC (VIABILITY OF EPIDIDYMAL SPERMATOZOA CROSSBREED CATTLE PRESERVED WITH COCONUT WATER AT 5oC)

2018 ◽  
Vol 18 (4) ◽  
pp. 571
Author(s):  
Muhammad Rizal ◽  
Muhammad Riyadhi ◽  
Bambang Irawan ◽  
Anis Wahdi ◽  
Habibah Habibah ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Saline Solution ◽  
Egg Yolk ◽  
Coconut Water ◽  
Randomized Design ◽  
Significant Difference ◽  
Epididymal Spermatozoa ◽  
Completely Randomized Design ◽  
Difference Test

The objective of this research was to examine the effectiveness of coconut water with various egg yolk concentrations in maintaining the viability of epididymal spermatozoa of crossbreed cattle preserved at 5oC. Five testis with epididymides of crossbreed cattle were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure methods of cauda epididymides with saline solution (0.9% NaCl). Collected-spermatozoa was equally divided in volume into four tubes and diluted with lactose extender containing 20% egg yolk (control), 90% coconut water + 10% egg yolk (CWEY10), 85% coconut water + 15% egg yolk (CWEY15), and 80% coconut water + 20% egg yolk (CWEY20), repectively. Diluted-spermatozoa was stored in refrigerator at 5oC. Quality of dilutedspermatozoa including percentages of motile spermatozoa (MS), live spermatozoa (LS), spermatozoa with intact plasma membrane (IPM) were evaluated every day during four days of storage. Data were analyzed by using completely randomized design with four treatments and five replicates. Means were compared with significant difference test at 0.05 significant level. Results of this study showed that mean of spermatozoa concentration, percentage of MS, percentage of LS, percentage of spermatozoa abnormal, and percentage of IPM of crossbreed cattle fresh epididymal spermatozoa were 1,414 million cell/ml, 72%, 85%, 9%, and 90%, respectively. At day-4 of the storage, percentages of MS, LS, and IPM of control (43, 52.2, 59.2%) and CWEY20 (42, 52, 59%) were significantly (P<0.05) higher than CWEY10 (33, 45.4, 52.8%) and CWEY15 (37, 50, 54.6%). In conclusion, lactose and CWEY20 extenders could be maintaining the quality of epidydimal spermatozoa of crossbreed cattle for three days preservation at 5oC and two days for CWEY10 and CWEY15.

scholarly journals Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation

2019 ◽  
Vol 11 (1) ◽  
pp. 117-124 ◽  
Author(s):  
Yonadiah Dwitya ◽  
Kartini Eriani ◽  
Hendra Saputra ◽  
Al-Azhar Al-Azhar ◽  
Muhammad Rizal
Keyword(s):  
Date Palm ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Phoenix Dactylifera ◽  
Dna Integrity ◽  
Randomized Design ◽  
Sperm Dna ◽  
Completely Randomized Design ◽  
Fresh Semen

Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.

Haematology and serum biochemical indices of broiler chickens fed varying levels of indomie waste meal based diet

2019 ◽  
Vol 7 (3) ◽  
Author(s):  
S. O. Omoikhoje ◽  
S. A. Eguaoje ◽  
J. Edonije ◽  
E. Osayande ◽  
J Edonije
Keyword(s):  
Broiler Chickens ◽  
Control Diet ◽  
Mean Value ◽  
Blood Profile ◽  
Randomized Design ◽  
Serum Biochemical ◽  
The Mean ◽  
Completely Randomized Design ◽  
Biochemical Indices ◽  
Haematological Indices

An eight-week feeding trial was conducted to evaluate the effect of substituting maize with indomie waste meal on the haematology and serum biochemical indices of 90-dayold Anak 2000 broiler chickens. Three experimental broiler starter and finisher diets were formulated with diet 1 serving as control containing 0% Indomie waste meal (IWM), while diets 2 and 3 contained IWM at 50 and 100% inclusion levels for maize. The chicks were randomly assigned to the three treatment diets (1, 2 and 3) in a completely randomized design and each treatment group contained three replicates with ten chickens per replicate. The result on the haematological indices revealed that MCV values were significantly influenced (p<0.05) by the treatment diets with highest value of (135.50±0.21fl) among birds fed 50% IWM. MCH also significantly varied(p<0.05) among birds fed the treatment diets with highest (42.77±0.31pg) recorded among birds maintained on the control diet. Serum biochemical indices revealed that phosphorus value were significantly higher (p<0.05) in birds fed the control diet with the mean value of 3.63±0.09mg/100ml. Total cholesterol was significantly higher (p<0.05) in birds fed 100% IWM. The overall results from this study conclusively suggest that the inclusion of indomie waste meal in the diet of broilers up to 100% did not have any detrimental effect on the blood profile of the birds. But precaution had to be taken in the incorporation of IWM at this level because of the increase effect on the cholesterol level of the blood.Mr. Omoikhoje, B. (Laboratory Technologist)

scholarly journals The Effect of Jamblang Leaves Extract (Syzygium cumini) Inclusion Skim Milk-Egg Yolk Extender on Motility, Abnormality and Viability of Aceh Cattle Spermatozoa Stored at 4ºC

2021 ◽  
Vol 45 (1) ◽  
pp. 6
Author(s):  
Cut Intan Novita ◽  
Rosna Sari ◽  
Eka Meutia Sari
Keyword(s):  
Standard Deviation ◽  
Research Result ◽  
Skim Milk ◽  
Egg Yolk ◽  
Mean Value ◽  
Syzygium Cumini ◽  
Microsoft Excel ◽  
Randomized Design ◽  
The Mean

This research aimed to know the effect of the addition of jamblang leaves extract (Syzygium cumini) in skim milk-egg yolk diluent material on motility, abnormality, and viability of Aceh cattle stored at 4ºC. The research design used was Complete Randomized Design consisting of 5 treatment and 5 repetitions. The treatments consisted of J0 (SSKT), J1 (SSKT+ EDJ 0. g/ 100 ml), J2 (SSKT+ EDJ 0.4 g/ 100 ml), J3 (SSKT+ EDJ 0.6 g/ 100 ml) and J4 (SSKT+ EDJ 0.8 g/ 100 ml). The data obtained were analyzed descriptively by determining the mean value and standard deviation using Microsoft Excel. The research result indicated that the addition of jamblang leaves extract in skim milk-egg yolk diluent material maintaining the motility, viability and abnormality of the spermatozoa of Aceh cattle stored at the temperature of 4ºC. Based on the research that has been carried out,  it can be concluded that the addition of jamblang leaves extract in skim milk-egg yolk diluent material can maintain the quality of Aceh cattle spermatozoa, while the use of incorrect dose becomes toxic and disrupt the spermatozoa activities to cause death.

scholarly journals Pengaruh Ekstrak Buah Lerak (Sapindus rarak) dan Beberapa Adjuvan terhadap Perkecambahan Gulma Fimbristylis miliacea

2021 ◽  
Vol 20 (2) ◽  
pp. 104
Author(s):  
Hidayat Pujisiswanto ◽  
Yayuk Nurmiaty ◽  
Nanik Sriyani ◽  
Annisa Efrima
Keyword(s):  
Mean Value ◽  
Polysorbate 80 ◽  
Fruit Extract ◽  
Randomized Design ◽  
Significant Difference ◽  
Bartlett Test ◽  
Homogeneity Of Variance ◽  
The Mean ◽  
Completely Randomized Design ◽  
And Control

Adjuvant is an ingredient added in a formulation to increase the effectiveness of lerak fruit in inhibiting weeds. This study aims to determine the type of adjuvant in lerak fruit extract that can increase the inhibition of germination of Fimbristylis miliacea and to determine the type of adjuvant in lerak fruit extract that is most effective in inhibiting the germination of F. miliacea. The research was conducted from December 2019 to March 2020 in the Weed Laboratory, Faculty of Agriculture, University of Lampung. This study used a Completely Randomized Design (CRD) to determine the type of adjuvant given to lerak fruit extract on F. miliacea germination with 4 replications. The treatments consisted of lerak fruit extract, lerak fruit extract + VCO adjuvant, lerak fruit extract + KAO adjuvant, lerak fruit extract + Polysorbate 80 adjuvant, and control. The Bartlett test was used to test the homogeneity of variance, if the assumptions of the analysis of variance were met, then the mean value of the treatment was continued with the Least Significant Difference (LSD) test at the 5% level. The results showed that adjuvants and without adjuvants added to lerak fruit extract at a concentration of 50% (500 g/l) were able to suppress the percentage of germination and the speed of germination of Fimbristylis miliace seeds.Keywords: adjuvants, lerak fruit extract, Fimbristylis miliacea, weed

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