scholarly journals Morphological changes in periodontal tissues under the influence of magnetophototherapy

2017 ◽  
pp. 38-47
Author(s):  
S.P. Rubnikovich ◽  
A.I. Maizet ◽  
Y.L. Denisova
Keyword(s):  
Morphological Changes ◽  
Periodontal Diseases ◽  
Polarized Light ◽  
Inflammatory Cells ◽  
Laboratory Animals ◽  
Control Group ◽  
Animal Testing ◽  
Noticeable Increase ◽  
Periodontal Tissues ◽  
The Impact

The effect of a low-intensity magnetic field combined with polarized light on the microvasculature links in periodontal tissues during orthopaedic treatment by fixed dentures in patients with partial secondary adentia and periodontal diseases were determined using an experiment on laboratory animals. The aim of the research was to study the character of morphological changes in the tissues of pathologically changed periodontium after exposure to magneto phototherapy in animal testing. Objects and methods. The experiment was made on selected 107 random-bred rats. The animals were divided into three groups: control group 1, control group 2, and the experimental group. Results and discussion. A dynamic analysis of morphological changes in the tissues of pathologically changed periodontium in three groups of animals demonstrated that, as compared to other animals, the rats from the experimental groups, following experimental magneto phototherapy, had early elimination of inflammation. Conclusion. Early elimination of inflammation (1 hour after termination of the impact) in all gum components and perifocal structures due to vaporization of inflammatory cells, activation of vessels (plethora and a noticeable increase in number) was found after the exposure to magneto phototherapy, while regeneration and complete epithelialization of the gingival pocket completed 2 days after termination of magneto phototherapy.

scholarly journals APPLICATION OF CELLULAR TECHNOLOGIES FOR REGENERATION OF PERIODONTAL TISSUES IN EXPERIMENT

2018 ◽  
Vol 62 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Sergei P. Rubnikovich ◽  
Igor D. Volotovsky ◽  
Yulia L. Denisova ◽  
Тatiana E. Vladimirskaya ◽  
Vasilina A. Andreeva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Morphological Changes ◽  
Fibrous Tissue ◽  
Laboratory Animals ◽  
Control Group ◽  
Cell Transplant ◽  
Periodontal Tissues ◽  
White Rats

A promising scope of modern scientific research is the use of autologous and allogeneic mesenchymal stem cells for regeneration of periodontal tissues. The aim of the study was to evaluate the nature of morphological changes in the pathologically altered periodontal tissues after injection of a biotransplant containing mesenchymal stem cells of the adipose tissue immobilized on a collagen carrier in an animal experiment. In the experiment, 60 randobbred females of white rats were used as a model, whose adipose tissue was taken to obtain allogenic mesenchymal stem cells. All animals were divided into 5 groups, depending on the planned method of treatment – 10 rats each. The control group consisted of 10 laboratory animals with healthy gingiva. The experimental gingival recession model was created by the V-shaped excision of periodontal tissues. The bioplastic collagen material “Collost” gel 7 % in isolated form determines the fibrosis intensification and serves as a “matrix” for the formation of a fibrous tissue, ensures the adhesion of stem cells and their transformation into pro- and fibroblasts. Injection of a cell transplant suspension into physiological saline activates the processes of cell proliferation and transformation of fibroblast differentiating cells. Suspension of the cell transplant on a sterile bioplastic collagen material “Collost” gel 7 % enhances the effects of gel and stem cells, promotes the leveling of atrophic and dystrophic changes in the gum, strengthening a mechanical component, reducing the recession of the gum and the depth of the gingival pocket.

Comparative analysis of polymorphic variants of IL-17A and MMP-1 genes with the risk of developing chronic periodontitis in petrochemical workers

2020 ◽  
Vol 60 (10) ◽  
pp. 687-693
Author(s):  
Iskander I. Zaidullin ◽  
Denis O. Karimov ◽  
Lilija K. Karimova ◽  
Milyausha F. Kabirova ◽  
Rasima R. Galimova ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Clinical Manifestations ◽  
Control Group ◽  
Periodontal Tissues ◽  
Dental Examination ◽  
Number Of Patients ◽  
Increased Risk ◽  
Harmful Substances

The susceptibility to the development and progression of inflammatory periodontal diseases, which depends on genetic and external factors (smoking, stress, oral hygiene), varies widely. In the development of these diseases, an important role is played not only by the presence of periodontal pathogenic microorganisms, but also by the presence of congenital or acquired immunodeficiency, immunoregulatory defects. The immune system plays a key role in the physiological and pathological processes of periodontal tissues. In this regard, IL17, produced by CD4+ Th cells, which has both Pro-inflammatory and protective activity, is of particular interest in the pathogenesis of periodontitis. The aim of study was to identify the relationship between polymorphic loci of the IL-17A (rs2275913) and MMP-1 (rs1799750) genes and clinical manifestations of chronic periodontitis in petrochemical workers. Dental examination was performed in 92 ethylene oxide production workers with chronic periodontitis and 74 patients with chronic periodontitis who did not come into contact with chemical factors (control group). Genotyping of polymorphisms rs2275913 of the IL17A gene and rs1799750 of the MMP1 gene was performed by allele-specific real-time polymerase chain reaction (PCR). Hygienic assessment of the degree of air pollution of the working area with harmful substances was carried out by gas chromatography according to the guidelines for the determination of harmful substances in the air № 5098-89, № 3119-84. When comparing the results of studies of both groups, there were no statistically significant differences in the frequency distributions of allelic variants and genotypes of the IL-17A and MMP-1 genes. The AA/AG genotypes of the IL-17A gene were associated with an increased risk of severe disease compared to the GG genotype in workers in the main group (OR=6.1; 95% CI 1.33-28.5; p=0.021) and in the control group (OR=7.26; 95% CI 1.34-39.25; p=0.016). Carriers of the A allele in the control group increased the risk of severe chronic periodontitis by 2.4 times compared to carriers of the G allele (OR=2.41; 95% CI 1.19-4.87; p=0.014). During the dental examination of employees of the ethylene oxide plant, the clinical course of periodontal diseases was more severe in comparison with the control group, and the number of patients with severe periodontitis was twice as high. It was found that the AA/AG genotypes of the IL-17A gene and the carrier of the A allele are associated with increased susceptibility to the development of severe chronic periodontitis. The association between the MMP-1 gene polymorphism and the risk of severe forms of chronic periodontitis has not been established. A risk factor for the development of inflammatory periodontal diseases in employees of the petrochemical complex is a complex of harmful production factors.

Hypoxia during pregnancy in rats leads to early morphological changes of atherosclerosis in adult offspring

2009 ◽  
Vol 296 (5) ◽  
pp. H1321-H1328 ◽  
Author(s):  
Zhenhua Wang ◽  
Ziyang Huang ◽  
Guorong Lu ◽  
Ling Lin ◽  
Markus Ferrari
Keyword(s):  
Morphometric Analysis ◽  
Time Course ◽  
Morphological Changes ◽  
Adult Life ◽  
Inflammatory Cells ◽  
Prenatal Hypoxia ◽  
Control Group ◽  
Adult Offspring ◽  
Sprague Dawley ◽  
Maternal Hypoxia

Exposure to an adverse intrauterine environment increases the risk of cardiovascular disease later in adult life. However, the time course relationship between prenatal hypoxia and the onset of atherosclerosis in offspring remains unknown. The purpose of this study is to evaluate the role of reduced fetal oxygen supply on early development of atherogenesis in the adult offspring and further assess its susceptibility to sex-, hyperlipidemia-, and postnatal hypoxemia-related differences. Based on a 4 × 2 full factorial design consisting of four factors of maternal hypoxia, sex, hyperlipidemia, and postnatal hypoxemia, characteristics of growth were determined, and histopathological observation and morphometric analysis of the thoracic aortas were performed in Sprague-Dawley rat offspring. Intrauterine growth restriction, altered body shape at birth, and accelerated postnatal weight gain occurred in the maternal hypoxia group but did not occur in the control group. In 16-mo-old maternal hypoxia offspring, the thoracic aortas exhibited lesions similar to early events in atherosclerosis that involved impaired endothelial cells, thickening and fibration of intimas, infiltration of inflammatory cells to the subendothelial space, and migration and proliferation of vascular smooth muscle cells to the intima. In contrast, no detectable pathological changes were observed in the offspring without maternal hypoxia exposure. Morphometric analysis further demonstrated that prenatal hypoxia caused a significant thickening of intima ( P < 0.001) with a main effect of 5.5 μm, an approximately twofold increase compared with controls. In addition, there was a positive additive relationship between prenatal hypoxia and hyperlipidemia on the intimal thickness ( P < 0.05). There were no other main effects or interaction among these four factors. In summary, our results indicate that maternal hypoxia during pregnancy leads to early pathological appearances of atherogenesis in adult offspring. This effect was enhanced with hyperlipemia but was unaffected by postnatal hypoxia or sex.

scholarly journals DISTURBANCE OF IRON METABOLISM AND ULTRASTRUCTURAL FEATURES OF THE GINGIVA IN PATIENTS WITH Β-THALASSEMIA MAJOR

2018 ◽  
Vol 22 (5) ◽  
pp. 255-261
Author(s):  
Ramida Vaqif qizi Shadlinskaya ◽  
E. K Gasimov ◽  
S. A Israfilova
Keyword(s):  
Iron Metabolism ◽  
Serum Iron ◽  
Binding Capacity ◽  
Periodontal Diseases ◽  
Thalassemia Major ◽  
Control Group ◽  
Periodontal Tissues ◽  
Metabolism Disorder ◽  
Pathogenetic Factor ◽  
Iron Binding Capacity

Introduction: β-thalassemia major is a hereditary blood disease, a main pathogenetic factor of which is the disruption of the synthesis of β-chains of hemoglobin. Inevitable metabolic disorders that occur during regular blood transfusion form an additional risk of damage and cellular modification of organs and tissues, including the oral cavity. Aim: determination of the pathogenetic role of iron metabolism disorder in the development of chronic inflammatory periodontal diseases in patients with β-thalassemia. Material and methods: 12 patients with β - thalassemia major who had periodontal diseases were examined in the Republican Center of Thalassemia in Baku (average age is 18 ± 1.3 years). The control group consisted of 16 somatically healthy individuals with intact gingiva tissues (the average age is 18.0 ± 0.7 years). Serum Iron, ferritin, hepcidin, total iron-binding capacity (TIBC), level of Il2, Il6, Il10, TNFα were measured from peripheral blood samples. To determine the ultra-structural study of the periodontal tissues in patients with β-thalassemia major, the method of electron microscopy studies of gingiva biopsies was used. Results of the study: The study revealed an increase in the level of serum iron and ferritin against the background of a progressively decreasing TIBC in patients with β-thalassemia major. Also activation of pro-inflammatory cytokines - TNFα, Il6, Il10 was observed in homozygous patients. Metabolic indicators are compared with ultrastructural features of cellular elements of a gum. Signs of accumulation of iron in cellular elements both of its own plate and of the epithelial cover were compared. The presence of iron was recorded in immunocompetent cells - macrophages and lymphocytes. Conclusion: Summarizing the data obtained, we can conclude that deproteinization of ferritin cores and their detection both in the cytoplasm and in the nucleoplasm of immunocompotent cells leads to irreversible degenerative changes in cellular elements of the gingiva. The authors link the development of the generalized inflammatory - degenerative process in the periodontium with revealed exchange -structural violations.

scholarly journals Combined effect of glyphosphate, saccharin and sodium benzoate on rats

2018 ◽  
Vol 9 (4) ◽  
pp. 591-597 ◽  
Author(s):  
M. A. Lieshchova ◽  
N. M. Tishkina ◽  
A. A. Bohomaz ◽  
P. M. Gavrilin ◽  
V. V. Brygadyrenko
Keyword(s):  
Body Weight ◽  
Sodium Benzoate ◽  
Food Additives ◽  
Combined Effect ◽  
Male Rats ◽  
Laboratory Animals ◽  
Relative Mass ◽  
Control Group ◽  
Expiry Date ◽  
The Impact

Herbicides and food additives are included in many food products for humans. Non-used products or products beyond their expiry date are deposited in places of utilizatioin of solid municipal wastes, where they can take effects on the organisms of mouse-like rodents. Among the herbicides, glyphosphate takes first place in the world for volume of production, and is the most intensely used in agricultural farming. The discussion about negative impact on the organisms of mammals, especially against the background of using various substances and environmental factors, continues. In this study, we determined the combined effect of glyphosphate and food additives on the organism of laboratory animals, which manifested in changes in body weight, condition and indices of mass of the internal organs and blood parameters. Four groups of laboratory male rats were formed, which over 42 days received: unlimited access to clean water; 1% aqueous solution of glyphosate; 1% solution of glyphosphate and 1% solution sodium benzoate; 1% solution of glyphosphate with 1% solution of saccharin. Glyphosphate and glyphosphate with sodium benzoate and saccharin significantly reduced the daily increases in body weight of animals compared to the control group. The studied substances have notable suppressive effect on the immune system and haematopoiesis in general, which is manifested in reduce of relative mass of the thymus and spleen against the background of increase in the amount of lymphocytes in the peripheral blood. The inhibition of haematopoiesis is indicated by decrease in the amount of erythrocytes, neurophils and hemoglobin of blood of animals from the experimental groups. The impact on the digestive system of glyphosphate and food additives is indicated by occurance of the effect of “irritation” of mucous membranes, and, as a result, disorders in absorption followed by the disorder in metabolic processes. A dysbalance occurs in enzymic systems of the organism, which is manifested in distrophic processes, especially in the liver parenchyma, indicated by the activity of blood enzymes (ALT, AST, alkaline phosphatase), total number and ratio of proteins of blood plasma. We determined the impact of glyphosphate and its mixes with benzoate and saccharin on the pancreas, which manifests in severe pancreatitis with steep increase in the level of glucose of blood. The results of the study allow us to state that mixture of glyphosphate and food additives can cause toxic effect in animals and humans, which often contact with herbicides.

scholarly journals Comparative evaluation of expression of VEGF (vascular endothelial growth factors) in periodontal tissues of healthy, chronic and aggressive periodontitis patients: an immunohistochemical study

2020 ◽  
Vol 9 (1) ◽  
pp. 784-788
Keyword(s):  
Growth Factors ◽  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Vascular Endothelial ◽  
Healthy Controls ◽  
Periodontal Tissues ◽  
Inflammatory Status

Periodontitis is a chronic inflammatory disease of the vascularized supporting tissues of the teeth. Angiogenesis (neovascularization) is the budding of new capillaries and is thought to be an essential process in the development of chronic inflammatory diseases. Inflamed tissues (such as gingiva coincident in periodontal disease) have evidence of enhanced expression of inflammatory mediators, many of which can promote angiogenesis. Of the various cytokines and growth factors that are involved in angiogenesis, the most potent agent that acts specifically on vascular epithelium is Vascular Endothelial Growth Factor. Even though angiogenesis is a prominent feature of both inflammation and healing, information about its role in periodontal lesions is limited. Hence the aim of the present study was the immunohistochemical evaluation of the expression of VEGF in the gingival tissues of chronic and aggressive periodontitis patients compared to the healthy controls. The present study was carried out in a total of 45 subjects with age range of 18-55 years, reporting to the Department of Periodontology, Rajarajeswari Dental College and Hospital, Bangalore. Gingival tissue samples were collected from all the 45 subjects and categorized into three groups based on their clinical findings as follows: Group 1 (Healthy), Group II (Chronic Periodontitis), Group III (Aggressive Periodontitis). Following sample collection, immunohistochemical staining of tissues was carried out and evaluation was done to compare the grades of expression of VEGF in the three groups. The expression of VEGF in blood vessels was also quantitatively evaluated. The results were statistically analyzed using Kruskal Wallis ANOVA and Mann Whitney test. There was a statistically significant higher expression of VEGF in both chronic periodontitis and aggressive periodontitis group as compared to the control group. Aggressive periodontitis cases showed higher grades of expression of VEGF compared to the chronic periodontitis cases and healthy controls. However, the difference in expression of VEGF was not statistically significant between the two forms of periodontitis. The presence of VEGF in both chronic and aggressive periodontitis clearly indicates the potent role played by VEGF as an inflammatory agent in the initiation and progression of periodontal diseases. Thus, VEGF might be used as a potential vascular marker for the assessment of severity and inflammatory status in periodontal disease.

scholarly journals THE INFLUENCE OF OZONE THERAPY ON THE MORPHOLOGIC CHANGES IN THE PATIENTS PRESENTING WITH PURULENT INFLAMMATION OF THE LUNGS IN THE EXPERIMENT

2017 ◽  
Vol 16 (3) ◽  
pp. 137-140
Author(s):  
Gulora A. Sadykova ◽  
Kh. U Rakhmatullaev ◽  
R. Sh Mavlyan-Khodjaev ◽  
Z. S Zalyalova ◽  
Yu. Kh Tadjikhodjaeva
Keyword(s):  
Respiratory Tract ◽  
Chronic Inflammation ◽  
Saline Solution ◽  
Negative Impact ◽  
Morphological Changes ◽  
Laboratory Animals ◽  
Control Group ◽  
Experimental Animals ◽  
Mechanical Irritation ◽  
Inflammation Of The Lungs

We have created the experimental model of chronic inflammation of lungs by means of prolonged mechanical irritation of the bronchi in 30 outbred rats and studied the morphological changes in the lung tissue of these animals rats in three series of experiments. Each rat was given an intraperitoneal injection of an ozonised saline solution produced by a «Binafsha» ozonator. The objective of the study was to compare a control group of healthy animals and the group of experimental animals with chronic purulent pneumonia. The prolonged irritation of the respiratory tract in experimental animals was found to induce the structural changes in the tissues of the lungs characteristic of chronic purulent inflammation. The course of treatment with the ozonised saline solution in healthy animals with experimentally modelled chronic purulent inflammation of the lungs did not have a negative impact on the general condition and the behaviour of the animals. The treatment of experimental chronic inflammation of lungs caused by prolonged mechanical irritation of the respiratory tract resulted in the improvement of the morphological status of the laboratory animals, but the purulent inflammation process failed to be completely resolved after the treatment which needs to be taken into consideration in the clinical practice.

The impact of the viral-bacterial consortium on occurrence and development of chronic periodontitis

2020 ◽  
Vol 25 (2) ◽  
pp. 84-89
Author(s):  
V. N. Tsarev ◽  
E. A. Yagodina ◽  
T. V. Tsareva ◽  
E. N. Nikolaeva
Keyword(s):  
Pathogenic Bacteria ◽  
Periodontal Diseases ◽  
Bacterial Consortium ◽  
Progressive Increase ◽  
Bacterial Biofilm ◽  
Herpes Simplex Viruses ◽  
Periodontal Tissues ◽  
Periodontal Inflammation ◽  
Immunological Mechanisms ◽  
The Impact

Relevance. The current theory of specific bacterial biofilm fails explain why a part of patients experiences inflammatory periodontal diseases while the absence of detected specific types of “red complex” bacteria.Purpose. To clarify the microbiological and immunological mechanisms of the influence of the viral and bacterial consortium in the etiology and pathogenesis of inflammatory periodontal diseases.Materials and methods. Articles survey with elements of metanalisis. Literature review based on discussion of research results on the topic of 48 sources including 33 foreign ones.Results. The review provides evidences of the possible participation of viruses of the Herpesviridae family in the development of chronic generalized periodontitis. Evidences for the role of herpes simplex viruses of type 1.2, Epstein-Barr virus, and cytomegalovirus in the development of periodontal inflammation are analyzed. It is proven that all herpesviruses induce the release of proinflammatory cytokines that activate osteoclasts and matrix metalloproteinases, as well as violate antibacterial immune mechanisms. In turn that leads to a progressive increase of periodontal pathogenic bacteria in both the biofilm and periodontal tissues.Conclusion. It is made a conclusion that an active herpetic infection can initiate damage to periodontal tissus and participate in the development of relapses of the disease.

scholarly journals Bufei Decoction Alleviated Bleomycin-Induced Idiopathic Pulmonary Fibrosis in Mice by Anti-Inflammation

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Shanjun Yang ◽  
Wenwen Cui ◽  
Mingye Wang ◽  
Luming Xing ◽  
Yue Wang ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Morphological Changes ◽  
Therapeutic Option ◽  
Signal Transduction Pathway ◽  
Inflammatory Cells ◽  
Control Group ◽  
Therapeutic Effects ◽  
Group Model ◽  
Anti Inflammation

Objective. This study aimed to investigate the mechanistic action and therapeutic effects of Bufei decoction on idiopathic pulmonary fibrosis (IPF) after inhalation of bleomycin. Methods. Pulmonary fibrosis model in mice was prepared by atomization inhalation of bleomycin. Then, the mice were randomly divided into five groups (control group, model group, positive group, and treatment group) and administrated the drugs for 4 weeks. H&E and Masson’s staining of lung tissues were used to observe the morphological changes and deposition of fibers, and the degree of fibrosis was evaluated by hydroxyproline content. The expression and activation of NF-κB were determined by western blotting and immunohistochemistry. The infiltration of macrophages was detected by immunostaining of CD45 and F4/80 in lung tissues. Results. In mouse IPF, Bufei decoction alleviated the pathological changes and the deposition of fibrosis by decreasing the content of hydroxyproline of lung tissues. The antipulmonary fibrosis might rely on the effects of preventing the infiltration of inflammatory cells and inhibiting the expression and activation of NF-κB in lung tissue. Conclusion. Bufei decoction improved the process of pulmonary fibrosis by regulating the activation and expression of the NF-κB signal transduction pathway, which provided a therapeutic option for IPF patients.

scholarly journals USING MESENCHYMAL STEM CELLS FOR TREATING GINGIVAL RECESSION IN THE EXPERIMENT

2018 ◽  
Vol 25 (5) ◽  
pp. 83-92
Author(s):  
S. P. Rubnikovich ◽  
Yu. L. Denisova ◽  
V. A. Andreeva ◽  
G. Yu. Panasenkova ◽  
I. S. Khomich
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Experimental Studies ◽  
Gingival Recession ◽  
Laboratory Animals ◽  
Control Group ◽  
Periodontal Tissues ◽  
White Rats ◽  
Clinical Changes ◽  
Complete Regeneration

Aim. This research was conducted to study the type of clinical changes in the diseased periodontal tissues by means of using mesenchymal stem cells for treating gingival recession in the experiment.Materials and methods. Adipose tissue in a volume of 1-2 ml was sampled from one specimen in sterile conditions in order to obtain allogeneic mesenchymal stem cells before the beginning of the experimental studies. Random-bred female white rats (60 specimens) were used as a model in the experiment. The surgical intervention was performed in the area of incisors on the upper and lower jaws of rats. All animals were divided into 5 groups (10 rats in each group) depending on the planned method of the treatment. The control group, intact rats, consisted of 10 laboratory animals with a healthy gingiva. The creation of a model of the experimental gingival recession was carried out by the mechanical V-shaped excision of periodontal tissues.Results. The experimental studies showed the possibility of complete regeneration of the dentogingival papillae as well as periodontal tissues regeneration in the area of the experimental gingival recession during the first 28 days. Meanwhile, there is a complete absence of inflammatory signs in the 5th group (hyperemia, edema, gingival bleeding), along with their significant decrease in the 3rd and the 4th groups that confirms the pronounced therapeutic effect of mesenchymal stem cells and sterile bioplastic collagen material «Collost» (7% gel).The conclusion. While studying the type of clinical changes in diseased periodontal tissues using mesenchymal stem cells in the treatment of gingival recession, we established the positive dynamics in the experiment. The decrease in the intensity and prevalence of gingival inflammation starts from the 14th day. On the 28th day there was noted a complete absence of inflammatory signs in the animals after injecting the transplant consisting of the cell suspension on the sterile bioplastic material Collost (7% gel). Furthermore, the significant increase in the regeneration of the gingival margin was observed on the 24th day from the moment of creating the gingival recession model. The complete regeneration of the dentogingival papillae and the absence of the gingival recession were noted on the 38th day of general observation. 

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