scholarly journals Combination Gel Formulation Extracts of Moringa Leaf and Red Betel Leaf as an Inhibitor of Acne-Causing Bacteria (Propionibacterium acne and Staphylococcus aureus)

2021 ◽  
Vol 8 (3) ◽  
pp. 18-23
Author(s):  
Eko Sri Wahyuningsih ◽  
Wahono Sumaryono ◽  
Chaidir Chaidir
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Chemical Parameters ◽  
Physical And Chemical Parameters ◽  
Gel Preparation ◽  
Physical And Chemical ◽  
And Storage ◽  
And Staphylococcus Aureus

The most common skin disorder is acne (Acne vulgaris) which is a chronic inflammatory disease that occurs in the pilosebaceous unit. Infection can be caused by Propionibacterium acnes and Staphylococcus aureus. Moringa leaves are known to inhibit acne growth. Piper crocatum leaves are also thought to have antibacterial activity. The study aims to determine the effect of the combination of the two extracts in inhibiting the growth of acne-causing bacteria, also tested the gel formula to meet physical and chemical parameters as well as stability to temperature and storage, also to test the acute dermal irritation against rabbit. Each extract with a concentration of 0.3%; 0.6%; 1.25%; 2.5%; 5%; 10%; 20%, incubated then measured the inhibition zone, then determined the minimum inhibition zone concentration of the two extracts to determine the lowest levels of the extracts which still gave antibacterial activity against the tested bacteria by the well diffusion method. The extract combination was formulated in a gel preparation with the excipients Carbopol 940, PG, Phenoxyethanol, TEA, CMC, and Aquadest. Antibacterial activity test for gel preparations used the well method with positive control Medi-Klin® gel. Then the formula evaluation includes physical, chemical, and microbiological evaluations. The results showed that the combination gel form had activity against P. acne and S. aureus at concentrations of 2.5% : 2.5%. The combination gel preparation of the two extracts also has synergistic effect in inhibiting acne bacteria. The formula can meet physical and chemical parameters and is stable to temperature and storage.

scholarly journals Antibacterial Activity Test of the Ethanol in Leaves Extract of Senduduk (Melastoma malabathricum L.) Against Escherichia coli and Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 139-152
Author(s):  
Alfi Sapitri ◽  
Nofita Lara ◽  
Panal Sitorus
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Wild Plants ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Melastoma Malabathricum ◽  
And Staphylococcus Aureus

Senduduk (Melastoma malabathricum L.) is one of the wild plants used as traditional medicine. Senduduk leaves are used as a medicine for sprue, boils, diarrhea and smallpox. The purpose of this study was to determine the antibacterial activity of the ethanol extract of senduduk leaves of the against Escherichia coli and Staphylococcus aureus. Senduduk leaves extracted by maceration using 96% ethanol solvent. Antibacterial activity testing is carried out using the diffusion method using paper discs.This experiment was repeated three times using concentrations of 20%, 40%, 60% and 80%.Ethanol extract of the senduduk leaves contains flavonoids, saponins, tannins and steroids/triterpenoids. The test results showed that the ethanol extract of the leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus which is characterized by the inhibition zone formed around the disc paper. In Escherichia coli bacteria the smallest inhibition zone at a concentration of 20% with a diameter of inhibition zone 11 mm and the largest diameter of the inhibitory zone at a concentration of 80% with a diameter of inhibition zone 19 mm. whereas for the smallest inhibitory zone Staphylococcus aureus bacteria at a concentration of 20% with an inhibition zone diameter of 12.6 mm and the largest inhibitory zone dameter at a concentration of 80% with a diameter of the inhibitory zone 21,3 mm.The results of the study concluded that the ethanol extract of Senduduk leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus.

scholarly journals Schiff bases derivatives: synthesis, identification, and antibacterial activity against pathogenic bacteria isolated from patients infected with wound infection

2020 ◽  
Vol 11 (3) ◽  
pp. 2993-2998
Author(s):  
Hawraa Mohammed Sadiq ◽  
Hutham Mahmood Yousif Al-Labban ◽  
Ahmed Abduljabbar Jaloob Aljanaby
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Wound Infection ◽  
Schiff Bases ◽  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
And Staphylococcus Aureus

Schiff bases derivatives are one of the most important compounds that have been used in many biological applications such as antimicrobial activity. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most important pathogenic bacteria that cause wound infection in Iraq and many developing countries. This research involves a synthesis of some Schiff bases compounds [A1 – A3] that were prepared from the condensation of [4- chlorobenzaldehyde, 4-bromobenzaldehyde, and 4-hydroxybenzaldehyde] in absolute ethanol. All of these compounds are characterized by [FTIR] spectroscopy. The antibacterial activity test was done according to agar well diffusion method for all derivative compounds with a concentration of 100 mg/ml and 200 mg/ml for each compound. The derivative compound A3 with a concentration of 200 mg/ml had an excellent antibacterial effect against Pseudomonas aeruginosa and Staphylococcus aureus with a diameter of inhibition zone of 18.417 ± 0.54645 and 16.000 ± 0.57735, respectively. Schiff bases derivatives synthesis compounds in this study (A1 and A2) can be considered as suitable antibacterial materials and can be used as raw materials to the synthesis of new ointment.

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

2017 ◽  
Vol 901 ◽  
pp. 124-132
Author(s):  
Artania Adnin Tri Suma ◽  
Tutik Dwi Wahyuningsih ◽  
Deni Pranowo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Shigella Flexneri ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

scholarly journals Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Megawati S. Saroinsong ◽  
Febby E. F. Kandou ◽  
Adelfia Papu ◽  
Marina F. O. Singkoh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Activity Test ◽  
And Staphylococcus Aureus ◽  
Paper Disc ◽  
Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

scholarly journals Antibacterial activity of different extract of medicinal plant Antirrihnum Orontium.

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Shehzad Saleem ◽  
Wisal Ahmad ◽  
Muhammad Hanif ◽  
Zahid Ali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Water Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Water Extracts ◽  
The Family ◽  
Quaternary Base ◽  
And Staphylococcus Aureus

Antirrhinum Orontium is belonging to the family Plantaginacea commonly known as lesser snapdragon which is a flowering plant belong to genus Antirrhinum. It was traditionally used in treatment of Tumor, Eye inflammation and Ulcer. Antirrhinum Orontium is contain mostly tertiary alkaloids bases one of them is identified as 4-methyl-2, 6-naphthyridine and Choline is identified as quaternary base. The recent study is to investigate the antibacterial activity of Ethyl acetate, Dichloromethane, Hexane and Water extracts of Antirrhinum Orontium, against the pathogen like Escherichia coli and Staphylococcus aureus. Both the Soxhlet and Maceration water extract were found most active from the other extract against Escherichia coli and Staphylococcus aureus by using agar well diffusion method. The measured zone of inhibition for Soxhlet water extract is 21±0.05mm and Maceration water extract is 20±0.05mm against gram positive Staphylococcus aureus, while for gram negative Escherichia coli 23±0.05mm is measured for Soxlet and 21±0.05mm measured for Maceration water extracts

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG KUNYIT (Curcuma longa L.) TERHADAP Staphylococcus aureus DAN Pseudomonas aeruginosa

2019 ◽  
Vol 4 (1) ◽  
pp. 278-287
Author(s):  
Lia Fikayuniar ◽  
Neni Sri Gunarti ◽  
Mellya Apriliani
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Curcuma Longa ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Paper Disk

ABSTRAK Kunyit (Curcuma longa L.) merupakan salah satu jenis tanaman obat yang termasuk dalam keluarga Zingiberaceae. Senyawa aktif yang terkandung dalam rimpang kunyit (Curcuma longa L.) mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol rimpang kunyit (Curcuma longa L.) terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Ekstraksi dilakukan dengan cara refluks menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi paper disk dengan masing-masing konsentrasi ekstrak 10%, 20%, 30%, 40% b/v. Kontrol positif yang digunakan adalah Ciprofloxacin sedangkan kontrol negatif yang digunakan adalah DMSO. Hasil skrining fitokimia ekstrak etanol rimpang kunyit mengandung alkaloid, flavonoid, fenol, tanin dan terpenoid. Berdasarkan hasil penelitian, ekstrak etanol rimpang kunyit dapat menghambat bakteri Staphylococcus aureus dan Pseudomonas aeruginosa pada konsentrasi 10%, 20%, 30%, 40% dan konsentrasi 40% merupakan konsentrasi yang memberikan diameter zona hambat terbesar terhadap kedua bakteri uji yaitu 8,63 mm dan 7,8 mm. Kata Kunci : aktivitas antibakteri, Curcuma longa L., Staphylococcus aureus,    ABSTRACT Turmeric (Curcuma longa L.) is one type of medicinal plant that belongs to the Zingiberaceae family. The active compounds contained in the Curcuma longa L. rhizome can work as antibacterial. This study aims to determine the antibacterial activity of the ethanol extract of Curcuma longa L. Antibacterial activity testing was carried out using the paper disk diffusion method with each extract concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin while the negative control used was DMSO. The results of phytochemical screening of the ethanol extract of Curcuma longa L. rhizome contain alkaloids, flavonoids, phenols, tannins and terpenoids. Based on the results of the study, the ethanol extract of turmeric rhizome can inhibit Staphylococcus aureus and Pseudomonas aeruginosa bacteria at concentrations of 10%, 20%, 30%, 40% and 40% concentrations which give the largest inhibition zone diameter of the two test bacteria which is 8.63 mm and 7.8 mm. Keywords: antibacterial activity, Curcuma longa L., Staphylococcus aureus,  

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals FORMULASI GEL EKSTRAK BATANG PEPAYA SEBAGAI ANTIBAKTERI TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS

2020 ◽  
Vol 4 (2) ◽  
pp. 1
Author(s):  
Amalia Eka Putri ◽  
Kristina Handayani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Stem Extract ◽  
Gel Preparation ◽  
To Come ◽  
Important Disease

Infectious disease is an important disease in Indonesia. Infectious diseases can be caused by pathogenic microorganisms such as the bacterium Staphylococcus aureus ATCC 25923. Infectious diseases can be treated with antibiotics, but antibiotics now resistance.Therefore, other alternatives such as are needed papaya stem which can be used as an antibacterial against S.aureus ATCC 25923. The purpose of this study was to determine the antibacterial activity of papaya stem extract gel against Staphylococcus aureus ATCC 25923. Papaya stem sample extracted using a method soxhletation with 96% ethanol. The extract was made into a gel dosage with a concentration of 5%. The positive control used is clindamycin and the negative control is gel without extract. The results of the papaya stem gel antibacterial activity test showed that the papaya stem gel had antibacterial activity against S.aureus ATCC 25923. The papaya stem extract gel preparation with a concentration of 5% had an average inhibition zone diameter of 18,56±0,51mm. The antibacterial activity is thought to come from the flavonoids, tannins, and saponins contained in the papaya stem fraction.

scholarly journals ANTIBACTERIAL ACTIVITY OF Samanea saman LEAF ETHANOL EXTRACT AGAINST Escherichia coli AND Staphylococcus aureus AND ITS TOTAL FLAVONOID AND PHENOLIC CONTENTS

Jurnal Kimia ◽  
2018 ◽  
pp. 121
Author(s):  
W. S. Rita ◽  
I M. D. Swantara ◽  
I. A. R. Astiti Asih ◽  
N.K. Sinarsih
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
E Coli ◽  
Phenolic Contents ◽  
Agar Disc ◽  
Samanea Saman ◽  
And Staphylococcus Aureus

Antibacterial activity of Samanea saman usually shows a positif correlation to the flavonoid and phenolic contents.  The aim of this study was to evaluate the antibacterial activity of the ethanol extract of Samanea saman against Escherechia coli and Staphylococcus aureus and determine the total flavonoid and phenolic contents of the extract. The extraction was done by ethanol 96% at room temperature. The antibacterial assay was conducted by agar disc diffusion method. The total flavonoid and phenolic contents were determined by UV-Vis Spectrofotometer with the standard of quersetin and galic acid, respectively. The extraction of 250 g of Samanea saman leaves resulted in 24.5 g of ethanol extracts. The ethanol extract showed a moderate inhibition of 8.33 mm towards E. coli and a strong inhibition of 13.6 mm towards S. aureus at the concentration of 4%. The Minimum Inhibitory Concentration (MIC) of the extract against E. coli and S.aureus were of 3% and 0.3%”, respectively. The total flavonoid and phenolic contents were successively 1233.2991 mg QE/100g and 2544.6154 mg GAE/100g. Keywords: Escherechia coli, flavonoid and phenolic content,  Samanea saman, Staphylococcus aureus

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