scholarly journals Histo-morphology of cutaneous papillomatosis in indigenous cattle

2019 ◽  
Vol 1 (17) ◽  
Author(s):  
U. Ayman ◽  
S. K. Das
Keyword(s):  
Morphological Changes ◽  
Microscopic Analysis ◽  
Viral Disease ◽  
Skin Lesions ◽  
Eosin Y ◽  
Body Parts ◽  
Indigenous Cattle ◽  
Study Results ◽  
Internal Core ◽  
Perineal Region

Background: Papillomatosis is a viral disease manifested with benign cutaneous growths (skin epithelium hyperplasia) in different body parts which is encountered in cattle, goat, dog, rabbit, horse, rodent and also in human. The purpose of the present study is to detect the histo-morphological changes of cutaneous papillomatosis in indigenous cattle. Methods: Ten (10) random samples from skin lesions of warts were collected from adult indigenous cattle (10 cattle) that were diagnosed as cutaneous papillomatosis by clinical examination at Veterinary Teaching Hospital, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. For gross observations, location of appearance of warts, color and size was taken into consideration. Immediately after collection of sample, they were preserved in Bouin’s fluid followed by dehydration, clearing and infiltration and finally embedded by paraffin wax. Then samples were sectioned using rotatory microtome and stained with Harris’s Haematoxylin and Eosin Y to facilitate microscopic study. Results: The results of present study revealed that cutaneous papillomas were mostly located on the head and neck, around eyes, under the chin and perineal region in indigenous cattle. Grossly, warts in cattle are bumpy or cauliflower-like masses of various sizes and numbers that projected from the skin having grey, rough, scaly, and dry surface. When incised, its cut surface has an external layer- keratinized epithelium and an internal core- moist white connective tissue. Histopathological changes indicate hyperplastic features of the affected skin layers of epidermis. Further, the microscopic analysis also revealed orthokeratotic hyperkeratosis, acanthosis and down word hyperplasia, fibrovascular area, keratohyalin granules and koilocytes at different layers of the affected skin. Conclusion: The results of this present study might be useful for the classification and treatment of this common malady based on its histological changes.

scholarly journals The Influence of Salt Solution on Morphological Changes in a Geosynthetic Clay Liner

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Mochamad Arief Budihardjo
Keyword(s):  
Salt Solution ◽  
Morphological Changes ◽  
Microscopic Analysis ◽  
Optical Microscope ◽  
Distilled Water ◽  
Nacl Solution ◽  
Geosynthetic Clay Liner ◽  
Clay Liner ◽  
Morphological Variations ◽  
Crystal Deposit

Morphological variations of geosynthetic clay liner (GCL) samples, hydrated with two different permeates, distilled water and NaCl solution (100 mM concentration), were observed in detail using microscopic analysis. After the GCL samples were hydrated with the NaCl solution, they were observed with an optical microscope. While the surface of the treated GCL samples was similar to the surface of the untreated GCL, a crystal deposit was found on the surface of the treated samples. Using a scanning electron microscope (SEM), a more solid appearance was observed for the bentonite particles contained in the GCL after the sample was hydrated with distilled water in comparison to the GCL sample that was hydrated with the NaCl solution. It appears that salt solution hydration results in less swelling of the bentonite particles. Furthermore, the energy-dispersive X-ray spectrometer (EDS) results showed that distilled water hydration had no effect on the distribution of the elements contained in the GCL samples. However, bound chlorine was observed, which demonstrated that the bentonite particles had absorbed the NaCl solution. In addition, changes in the hydraulic conductivity of the hydrated GCL samples were also observed.

scholarly journals Toxicity and Teratogenic Effects of Zinc Sulfide Nanoparticles on Chick Embryo and Chick Fibroblast Cell Culture

2019 ◽  
Vol 25 (4) ◽  
pp. 270-281
Author(s):  
Asadollah Asadi ◽  
◽  
Arash Abdolmaleki ◽  
◽  
Keyword(s):  
Cell Culture ◽  
Zinc Sulfide ◽  
Morphological Changes ◽  
Lethal Dose ◽  
Fibroblast Cell ◽  
Zns Nanoparticles ◽  
Culture Methods ◽  
Study Results ◽  
Zinc Sulfide Nanoparticles ◽  
Fibroblastic Cells

Aims Nanoparticles (particles with a diameter of 10-500 nm) are currently used in the cosmetics industry as well as for pharmaceuticals, diagnostic imaging, and tissue engineering. Since these nanoparticles are used in industry and drug delivery, they can also be used by pregnant women. Thus, the current study investigated the teratogenic and cytotoxic effects of Zinc Sulfide (ZnS) nanoparticles on the embryo and their fibroblastic cell culture. Methods & Materials Zinc sulfide (ZnS) nanoparticles were synthesized. Then, nanoparticles at the concentrations of 5, 10, 15, 30, and 40 mg/mL/egg were injected into the air sac of the eggs in three replicates on the third day of incubation. Next, the treated and control eggs, on day 19 of incubation were opened, and embryos were weighted, and the relevant mortality rate was recorded. Fibroblast cells were isolated, cultured, and treated from the control embryo, and morphological changes and cell survival percentages were recorded. Findings The obtained results revealed that the embryos’ survival rate depends on the nanoparticle concentration. As a result, at the highest concentration, only 36.32% of the embryos survived, and the lethal dose 50% (LD50) was equal to 32.47 mg/egg. Morphological study of the treated embryos club foot and skeletal staining suggested the deletion of caudal vertebrate. The cytotoxicity study results of ZnS nanoparticles on fibroblastic cells indicated the survival fractions of 88.45%, 68.75%, and 49.32%, respectively, and its IC50 value was measured aas1460 μM. Conclusion The present study results suggested that ZnS nanoparticles had no significant toxic effects on the embryos and culture of chicken fibroblastic cells at low concentrations.

scholarly journals Antimycobacterial potential of the juniper berry essential oil in tap water

2018 ◽  
Vol 69 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Dolores Peruč ◽  
Ivana Gobin ◽  
Maja Abram ◽  
Dalibor Broznić ◽  
Tomislav Svalina ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mycobacterium Avium ◽  
Morphological Changes ◽  
Tap Water ◽  
Microscopic Analysis ◽  
Water Systems ◽  
Hot Water ◽  
Gas Chromatography Mass Spectrometry ◽  
Mediterranean Plants ◽  
Common Juniper

Abstract Mycobacterium avium complex-related diseases are often associated with poorly maintained hot water systems. This calls for the development of new control strategies. The aim of this study was to investigate the activity of essential oils (EOs) from the Mediterranean plants, common juniper, immortelle, sage, lavandin, laurel, and white cedar against Mycobacterium avium ssp. avium, Mycobacterium intracellulare, and Mycobacterium gordonae in culturing broth and freshwater as their most common habitat. To do that, we developed a new method of water microdilution to determine their minimal effective concentrations (MEC). The most active EO was the one from the common juniper with the MEC of 1.6 mg mL-1. Gas chromatography / mass spectrometry the juniper EO identified monoterpenes (70.54 %) and sesquiterpenes (25.9 %) as dominant component groups. The main monoterpene hydrocarbons were α-pinene, sabinene, and β-pinene. The juniper EO significantly reduced the cell viability of M. intracellulare and M. gordonae at MEC, and of M. avium at 2xMEC. Microscopic analysis confirmed its inhibitory effect by revealing significant morphological changes in the cell membrane and cytoplasm of all three bacteria. The mode of action of the juniper EO on the cell membrane was confirmed by a marked leakage of intracellular material. Juniper EO has a great practical potential as a complementary or alternative water disinfectant in hot water systems such as baths, swimming pools, spa pools, hot tubs, or even foot baths/whirlpools.

Physical and morphometric characteristics of Gangatiri cattle

2016 ◽  
Author(s):  
Basant Kumar Bhinchhar ◽  
Vinod Kumar Paswan ◽  
Satya Prakash Yadav ◽  
SPACE Saroj ◽  
Prity Singh
Keyword(s):  
Uttar Pradesh ◽  
Medium Size ◽  
Average Height ◽  
Body Parts ◽  
Indigenous Cattle ◽  
Face Width ◽  
Face Length ◽  
River Ganga ◽  
Shallow Groove ◽  
Eastern Uttar Pradesh

Gangatiri cattle are mainly found in the adjacent areas of the river Ganga in eastern Uttar Pradesh (Chandauli, Ghazipur, Varanasi, Mirzapur and Ballia districts) and adjacent Bhabhua (Kaimoor), Buxar, Arrah and Chhapra areas of Bihar. The breeding tract of this breed includes mainly Ballia and Ghazipur districts of Uttar Pradesh and Rohtas and Shahbad Districts of Bihar. Present study was conducted on 114 Gangatiri cattle - an indigenous cattle herd - maintained at Surbhi Shodh Sansthan (Geeta Goshala), Dagmagpur, Mirzapur, Uttar Pradesh- to study the physical and morphometric characterstics. White colour was the most prominant colour in the population. Colour of coat, forehead and neck was white and eyelid, tail and hoof were black in 65.78, 76.31, 63.15, 66.66, 82.45 and 76.31 % of animals, respectively. Hair was mainly straight type (97.10 %) but 2.90 % of the animals also had curly type of hair. Medium size muzzle was present in 79.71 % of the animals while it was small and large sized in 11.59 and 8.69 % of animals, respectively. Shades of grey were also present on different body parts of these animals. Grey colour was the second most prominant colour in the population. Majority of animals have shallow groove in middle head (57.97 %). Poll was prominent in 92.75 % of the population. Forehead was straight type in almost 78.26 % of these animals. Most of the adult animal population (72.46 %) was having medium body size. The average height at wither, body length, heart girth, face length, face width, neck length, ear length, fore cannon bone girth, height at hip bone, height at pin bone, rump slope and rump length were 117.12±1.23, 108.06±0.90, 145.50±1.80, 30.14±0.86, 15.48±0.43, 47.07±1.25, 19.99±0.56, 15.65±0.40, 117.78±1.16, 105.25±1.10, 12.47±0.16 and 35.56±0.65 cm, respectively.

scholarly journals Hataedock Treatment Has Preventive Therapeutic Effects in Atopic Dermatitis-Induced NC/Nga Mice under High-Fat Diet Conditions

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Ho-Yeol Cha ◽  
Sang-hyun Ahn ◽  
Jin-Hong Cheon ◽  
In-Sik Park ◽  
Jin-Tack Kim ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Morphological Changes ◽  
Skin Lesions ◽  
Control Group ◽  
Therapeutic Effects ◽  
High Fat ◽  
Coptidis Rhizoma ◽  
Inflammatory Regulation ◽  
Th2 Differentiation ◽  
Old Mice

This study investigated the preventive therapeutic effects of Hataedock (HTD) treatment on inflammatory regulation and skin protection in AD-induced NC/Nga mice under high-fat diet conditions. Before inducing AD, the extract ofCoptidis RhizomaandGlycyrrhiza uralensiswas administered orally to the 3-week-old mice. After that, AD-like skin lesions were induced by applying DNFB. All groups except the control group were fed a high-fat diet freely. We identified the effects of HTD on morphological changes, cytokine release and the induction of apoptosis through histochemistry, immunohistochemistry, and TUNEL assay. HTD downregulated the levels of IL-4 and PKC but increased the levels of LXR. HTD also suppressed the mast cell degranulation and release of MMP-9, Substance P. The levels of TNF-α, p-IκB, iNOS, and COX-2 were also decreased. The upregulation of inflammatory cell’s apoptosis is confirmed by our results as increase of apoptotic body and cleaved caspase-3 and decrease of Bcl-2. HTD also reduced edema, angiogenesis, and skin lesion inflammation. Our results indicate HTD suppresses various inflammatory response on AD-induced mice with obesity through the regulation of Th2 differentiation and the protection of lipid barrier. Therefore, HTD could be used as an alternative and preventive therapeutic approach in the management of AD.

Ballast Flying DEM Microscopic Analysis and Counteracting Measures

2011 ◽  
Vol 97-98 ◽  
pp. 266-270 ◽  
Author(s):  
Guo Qing Jing ◽  
Liang Gao ◽  
Xiao Lin Sun
Keyword(s):  
Friction Coefficient ◽  
Microscopic Study ◽  
High Speed ◽  
Microscopic Analysis ◽  
Dynamic Force ◽  
High Speed Railway ◽  
Model Method ◽  
Study Results

Ballast flying problems obsess the high speed railway developments, causing damages to the train and deteriorations to the rail. In the paper, DEM is used to conduct ballast flying mechanism microscopic study, results show ballast flying increases with train dynamic force, and decreases with ballast size and friction coefficient, ballast flying counteracting measures are presented, such as ballast bed parameter and ballast glue. In the end, future possible microscopic ballast flying model method is presented by DEM coupled with CFD.

scholarly journals Role of antibiotic in bronchiolitis management

2015 ◽  
Vol 9 (2) ◽  
pp. 70-76
Author(s):  
Khaleda Akhtar ◽  
Rehana Begum Chowdhury ◽  
Md Tauhidur Rahman
Keyword(s):  
Hospital Setting ◽  
Supportive Therapy ◽  
Armed Forces ◽  
Viral Disease ◽  
Control Group ◽  
Commanding Officer ◽  
Presenting Symptoms ◽  
Study Results ◽  
Randomized Control

Introduction: Bronchiolitis is the most common illness among the patients attending the outpatient departments of CMH. It is predominantly a viral disease affecting the infants and young children. Though Antibiotic has little role, pediatricians frequently use them during bronchiolitis management. Very few randomized control trials without antibiotics in the management of bronchiolitis have so far been done. Objectives: To evaluate the outcome of bronchiolitis with or without antibiotics in a hospital setting. Methods: This prospective randomized control study was done in CMH Savar, during six months from October 2012 to March 2013. All the children below two years admitted in CMH Savar with first attack of Clinical Bronchiolitis were our study population. Exclusion criteria were: (i) atopic condition, (ii) congenital heart disease and/or (iii) known immunodeficiency. Study cases were randomly assigned into one of the two groups, AB group (Erythromycin/Amoxycilin) and NAB group (No Antibiotic).The NAB group was considered as control group. Supportive treatment was given according to the national guideline for management of bronchiolitis. Presenting symptoms and signs were followed-up twice daily while hospitalized and 7 days after discharge to determine the progress of disease. 70 JAFMC Bangladesh. Vol 9, No 2 (December) 2013 Outcome was determined by the progress of the variables in the structured follow-up format. Permission of commanding officer CMH Savar and verbal consent of the parents were taken before the study. Results: Fifty-four cases who could be followed up till after seven days of discharge were finally included in the study. Among them about half (25/54) received oral or intravenous antibiotic while rest (29/54) received only supportive therapy but no antibiotic (NAB group). Most of the cases were below six months of age. Male were about double of the female (37:17). The presenting features were cough, wheeze, fever and feeding difficulty. Clinical features of both groups progressed similarly in both the groups. With the given treatment 24 (96%) cases from AB group and 27 (93%) cases from NAB group improved and were discharged safely. 01 from AB and 02 from NAB group deteriorated and were then treated with broad spectrum antibiotics. There was no death. Mean hospital stay of AB group (5.6 days) was little longer than NAB group (4.2 days) and 16% (4/25) of them had respiratory symptoms at seven days follow up, but the difference of outcome between the two groups was not statistically significant. Conclusion: Antibiotics have no role in acute bronchiolitis management. DOI: http://dx.doi.org/10.3329/jafmc.v9i2.21833 Journal of Armed Forces Medical College Bangladesh Vol.9(2) 2013

scholarly journals First report of Dermatophilus congolensis infection in native Omani cattle.

2020 ◽  
Vol 24 ◽  
pp. 34
Author(s):  
Mahmoud El-Neweshy ◽  
Julanda Al Mawly ◽  
Haytham Alieldin Ali
Keyword(s):  
Mononuclear Cells ◽  
Bacterial Culture ◽  
Skin Lesions ◽  
Skin Scraping ◽  
First Report ◽  
Gram Staining ◽  
Native Cattle ◽  
Perineal Region ◽  
Long Acting ◽  
Head Neck

Skin lesions were reported in a group of quarantined Omani native cattle for exportation purpose during January 2018. The skin lesions were reported in 17 animals out of 140 and were scattered over the head, neck, back and perineal region of the animals. Lesions varied from paint-brush matted hairs to wart-like lesions that are up to 2 cm in diameter. Skin scraping samples were obtained from the living animals and were directly stained with gram stain. Histologically, there was severe multifocal exudative dermatitis with hyperkeratosis. Dermis was infiltrated with neutrophils, mononuclear cells, and numerous branching filaments coccoid bodies. Bacterial culture on sheep blood agar revealed gray to white, beta hemolytic, raised and irregularly shaped colonies. Gram staining showed gram positive branching filaments with both transverse and longitudinal fragments formed from packs of coccoid cells confined with Dermatophilus congolensis. Animals were treated successfully with 3 doses of intra muscular injection of long-acting oxytetracycline with topical application of antibiotic spray. To the best of our knowledge, this is the first report of dermatophilosis in Omani cattle.

scholarly journals Epidemiological Study of Hand, Foot and Mouth Disease in Al-Kindy Teaching Hospital/ Baghdad

2019 ◽  
Vol 14 (2) ◽  
pp. 84-88
Author(s):  
Suha N. Al-Wakeel ◽  
Khansa A. Al Rubiae ◽  
Suha N. Al-Wakeel ◽  
Basil M. Hanoudi
Keyword(s):  
Clinical Features ◽  
Teaching Hospital ◽  
Specific Treatment ◽  
Foot And Mouth Disease ◽  
Viral Disease ◽  
Skin Lesions ◽  
Mouth Disease ◽  
Coxsackie Virus ◽  
Cross Sectional ◽  
Foot And Mouth

Background: Hand, foot, and mouth disease is viral disease caused commonly by coxsackie virus A16 virus. It is a mild disease and children usually recover with no specific treatment within 7 to 10 days. Rarely, this illness may be associated with aseptic meningitis were patient may need hospitalization. Objective: To determine significance of clinical features of hand, foot and mouth disease. Methods: A cross sectional study of cases with clinical features of hand, foot and mouth disease visiting the dermatological consultation unit of Al Kindy teaching hospital. Sampling was for Zyona and Edressi Quarter patients over the period of 1st December 2017 to 30th of November 2017. Aim: To determine significance of clinical features of hand, foot and mouth disease Results: The mean age of patients (100 patients) was 29.99 months. Males were 65 (65%) and females were 35 (35%), (P 0.23). Tenderness of skin lesions, Malaise and decreased Appetite were the most frequent symptoms. Winter months illness was common .Cases were diagnosed two days before seeking medical help, while home contact of patients was most common place for infection transmission .Involvement of palms and soles was universal and indifferent (100%). Groin was more commonly affected (67%), (P 0.015). Fever was present in 81%.

scholarly journals MORPHOLOGICAL VARIABILITY OF GRASS CARP CTENOPHARYNGODON IDELLA (VALENCIENNES, 1844) IN RESERVOIRS OF SOUTHERN KAZAKHSTAN

2020 ◽  
Vol 2020 (4) ◽  
pp. 22-28
Author(s):  
Olga Ivanovna Kirichenko ◽  
Saule Zhangirovna Asylbekova ◽  
Kuanysh Baibulatovich Isbekov
Keyword(s):  
Grass Carp ◽  
Morphological Variability ◽  
Morphological Features ◽  
Population Variability ◽  
Ctenopharyngodon Idella ◽  
High Plasticity ◽  
Body Parts ◽  
Adaptive Capabilities ◽  
Study Results ◽  
Grass Carp Ctenopharyngodon Idella

The article presents the study results of the inter-population variability of morphometric features of grass carp in the natural reservoirs of southern Kazakhstan (the Ili Delta and the Kapshagai Reservoir) and in the Kapshagai spawning and breeding farm. In the compared populations of herbivorous fish there has been found the variability in meristic characteristics to varying degrees, as well as the proportions of individual body parts. The increased value of inter-population variability may indicate the high plasticity of morphological features of grass carp and the significant adaptive capabilities of the compared populations. The valid differences in grass carp samples marked by a group of morphological features (plastic and meristic) and taken of fish from the hatchery and natural reservoirs of the Balkhash-Ili basin indicate the existence of species produced by artificial reproduction and by natural spawning in the natural reservoirs.

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