First report of Dermatophilus congolensis infection in native Omani cattle.

Skin lesions were reported in a group of quarantined Omani native cattle for exportation purpose during January 2018. The skin lesions were reported in 17 animals out of 140 and were scattered over the head, neck, back and perineal region of the animals. Lesions varied from paint-brush matted hairs to wart-like lesions that are up to 2 cm in diameter. Skin scraping samples were obtained from the living animals and were directly stained with gram stain. Histologically, there was severe multifocal exudative dermatitis with hyperkeratosis. Dermis was infiltrated with neutrophils, mononuclear cells, and numerous branching filaments coccoid bodies. Bacterial culture on sheep blood agar revealed gray to white, beta hemolytic, raised and irregularly shaped colonies. Gram staining showed gram positive branching filaments with both transverse and longitudinal fragments formed from packs of coccoid cells confined with Dermatophilus congolensis. Animals were treated successfully with 3 doses of intra muscular injection of long-acting oxytetracycline with topical application of antibiotic spray. To the best of our knowledge, this is the first report of dermatophilosis in Omani cattle.

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Juvenile sterile granulomatous dermatitis (puppy strangle) in Pekingese and German shepherd puppies

Veterinary Science Development ◽

10.4081/vsd.2015.6037 ◽

2015 ◽

Vol 5 (1) ◽

Author(s):

Mohammad Abbaszadeh Hasiri ◽

Efat Baghaei Moghaddam

Keyword(s):

Case Report ◽

Antibiotic Therapy ◽

Bacterial Culture ◽

Skin Lesions ◽

Response To Therapy ◽

Skin Scraping ◽

German Shepherd ◽

Present Case Report ◽

The Face ◽

Granulomatous Dermatitis

Juvenile sterile granulomatous dermatitis is an uncommon granulomatous and pustular disorder of the face, pinnae, and submandibular lymph nodes of puppies. A 10-week-old male Pekingese and a 8-week-old female German shepherd presented with submandibular lymphadenomegaly, skin lesions on muzzle and periocular area (Papules, crusts and pustules). The case did not respond to antibiotic therapy. Results of a hemogram, biochemical panel, and urinalysis were normal. Due to skin scraping, cytology examination (impression smear), fungal and bacterial culture and response to therapy puppy strangle (juvenile cellulitis) was diagnosed. The puppies made a full recovery on glucocorticoid therapy. The present case report describes the first report of juvenile sterile granulomatous dermatitis in Iran.

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Occurrence of dermatomycosis (ringworm) due to Trichophyton verrucosumin dairy calves and its spread to animal attendants : case report

Journal of the South African Veterinary Association ◽

10.4102/jsava.v69i4.848 ◽

1998 ◽

Vol 69 (4) ◽

Cited By ~ 4

Author(s):

J.K. Wabacha ◽

G.K. Gitau ◽

L.C. Bebora ◽

C.O. Bwanga ◽

Z.M. Wamuri ◽

...

Keyword(s):

Case Report ◽

Topical Treatment ◽

Skin Lesions ◽

Dairy Calves ◽

Response To Treatment ◽

Trichophyton Verrucosum ◽

Dairy Animals ◽

Hairless Skin ◽

Anti Fungal ◽

Head Neck

Persistent dermatomycosis (ringworm) caused by Trichophyton verrucosum affected 20 dairy calves aged between 3 months and 1 year and housed together. The infection also spread to 2 animal attendants working among the calves. The major clinical lesions observed on the affected calves were extensive alopecia and/or circumscribed thick hairless skin patches affecting the head, neck, flanks and limbs. The observed lesions persisted for more than 17 weeks and most of the calves did not respond to topical treatment with various anti-fungal drugs within the anticipated period of 9 weeks. Two animal attendants developed skin lesions that were circumscribed and itchy and there was good response to treatment following the application of anti-fungal skin ointment. Although ringworm in dairy animals in Kenya has not previously been associated with spread to humans, the potential is evident from this report.

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Histo-morphology of cutaneous papillomatosis in indigenous cattle

10.33109/bjvmjj19fam2 ◽

2019 ◽

Vol 1 (17) ◽

Author(s):

U. Ayman ◽

S. K. Das

Keyword(s):

Morphological Changes ◽

Microscopic Analysis ◽

Viral Disease ◽

Skin Lesions ◽

Eosin Y ◽

Body Parts ◽

Indigenous Cattle ◽

Study Results ◽

Internal Core ◽

Perineal Region

Background: Papillomatosis is a viral disease manifested with benign cutaneous growths (skin epithelium hyperplasia) in different body parts which is encountered in cattle, goat, dog, rabbit, horse, rodent and also in human. The purpose of the present study is to detect the histo-morphological changes of cutaneous papillomatosis in indigenous cattle. Methods: Ten (10) random samples from skin lesions of warts were collected from adult indigenous cattle (10 cattle) that were diagnosed as cutaneous papillomatosis by clinical examination at Veterinary Teaching Hospital, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. For gross observations, location of appearance of warts, color and size was taken into consideration. Immediately after collection of sample, they were preserved in Bouin’s fluid followed by dehydration, clearing and infiltration and finally embedded by paraffin wax. Then samples were sectioned using rotatory microtome and stained with Harris’s Haematoxylin and Eosin Y to facilitate microscopic study. Results: The results of present study revealed that cutaneous papillomas were mostly located on the head and neck, around eyes, under the chin and perineal region in indigenous cattle. Grossly, warts in cattle are bumpy or cauliflower-like masses of various sizes and numbers that projected from the skin having grey, rough, scaly, and dry surface. When incised, its cut surface has an external layer- keratinized epithelium and an internal core- moist white connective tissue. Histopathological changes indicate hyperplastic features of the affected skin layers of epidermis. Further, the microscopic analysis also revealed orthokeratotic hyperkeratosis, acanthosis and down word hyperplasia, fibrovascular area, keratohyalin granules and koilocytes at different layers of the affected skin. Conclusion: The results of this present study might be useful for the classification and treatment of this common malady based on its histological changes.

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Topical treatment of multiple erosive, ulcerative skin lesions in an Indian rhinoceros (Rhinoceros unicornis)

Tierärztliche Praxis Ausgabe G Großtiere / Nutztiere ◽

10.1055/a-1475-3459 ◽

2021 ◽

Vol 49 (03) ◽

pp. 210-214

Author(s):

Teresa M. S. A. Boehm ◽

Christoph J. Klinger ◽

Christine Gohl ◽

Maike Lücht ◽

Katja N. Baumann ◽

...

Keyword(s):

Fatty Acids ◽

Plant Extracts ◽

Bacterial Culture ◽

Essential Fatty Acids ◽

Clinical Signs ◽

Skin Lesions ◽

Product Line ◽

Topical Product ◽

Dermatological Examination ◽

Systemic Antibiotic Therapy

AbstractA 30-year-old, intact female Indian rhinoceros (Rhinocerus unicornis) was presented with ongoing erosive, ulcerative skin lesions over a 4-year-period. The lesions appeared to be non-pruritic and non-seasonal. A systemic antibiotic therapy had been unsuccessful. The dermatological examination showed 8 diffusely demarcated areas of erosion, focal ulceration, mild crusting, and moderate erythema ranging from 8 to 20 cm in diameter, bilaterally on the lateral edges of the torso armor plates. The patient had no other clinical abnormalities. Coccoid and rod-shaped bacteria were identified on cytology and a bacterial culture revealed Escherichia coli spp., Staphylococcus dysgalacticae, Stenotrophomonas maltophilia, Corynebacterium spp. and Micrococus spp. A topical product line containing essential fatty acids and plant extracts was administered daily, using a spot-on, spray and balm. Within 3 weeks a substantial alleviation of clinical signs was observed. Multiple impression smears of the lesions and a bacterial culture were negative by day 21. The patient achieved complete remission within 4 months of treatment and maintained remission for the 2-year observation period with continued use of the medication. Topically administered therapeutics containing essential fatty acids and plant extracts may offer a viable treatment option for recurrent cases of bacterial infectious skin lesions in Indian rhinocerotidae.

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γ-Interferon Production in Peripheral Blood Mononuclear Cells and Tumor Infiltrating Lymphocytes From Kaposi's Sarcoma Patients: Correlation With the Presence of Human Herpesvirus-8 in Peripheral Blood Mononuclear Cells and Lesional Macrophages

Blood ◽

10.1182/blood.v91.3.968 ◽

1998 ◽

Vol 91 (3) ◽

pp. 968-976 ◽

Cited By ~ 83

Author(s):

Maria Caterina Sirianni ◽

Laura Vincenzi ◽

Valeria Fiorelli ◽

Simone Topino ◽

Enrico Scala ◽

...

Keyword(s):

Cell Cultures ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Human Herpesvirus ◽

Tumor Infiltrating Lymphocytes ◽

Skin Lesions ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Infiltrating Lymphocytes

Abstract Evidence indicates that, at least in the early stage, Kaposi's sarcoma (KS) is a cytokine-mediated disease and that it is consistently associated with a novel herpesvirus termed human herpesvirus-8 (HHV-8). To gain insights into the mechanisms by which cytokines and HHV-8 may cooperate in disease pathogenesis, we examined the phenotype, the Th1 (γ-interferon [γIFN]) and Th2 (interleukin-4 [IL-4]) cytokine profile and the presence of HHV-8 in peripheral blood mononuclear cells (PBMC), tumor-infiltrating lymphocytes (TIL), and spindle cell cultures derived from skin lesions of patients affected by classical KS (C-KS) and acquired immunodeficiency syndrome (AIDS)-associated KS (AIDS-KS). TIL and spindle cell cultures were examined at day 0 or after culture in conditioned media from activated T cells (TCM) that contain the same cytokines increased in KS tissues. No differences were found in the immunophenotype of PBMC from C-KS patients versus controls, except for AIDS-KS patients who showed a T-CD8+ expansion. However, a preferential infiltration of T-CD8+ cells was found in all KS lesions examined, which was maintained after culture of TIL in TCM. γIFN production was found in both PBMC and cultures derived from all KS examined; some IL-4 positive supernatants were found only in three AIDS-KS cases. Uninvolved skin did not show appreciable lymphocyte infiltration or cytokine production. The culture conditions of the lesional skin allowed also the appearance of adherent, spindle-like cells bearing markers of tissue macrophages. Finally, most or all of the PBMC, lesions, and macrophagic cell cultures from the skin lesions were found to be positive for HHV-8 infection by nested polymerase chain reaction (PCR). These findings indicate that patients with KS express a Th1 phenotype with a prevalent γIFN production, likely accounted for by the local T-CD8+ infiltration. By analogy with other viral infections (ie, Epstein-Barr virus), this suggests that in loco recruitment of lymphoid cells and the subsequent γIFN production may be in response to or elicited by HHV-8 that was found in both PBMC and macrophagic cell cultures from the lesions of the same patients.

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Amyopathic dermatomyositis combined with peripheral neuropathy

BMJ Case Reports ◽

10.1136/bcr-2020-237250 ◽

2020 ◽

Vol 13 (11) ◽

pp. e237250

Author(s):

Zenshi Miyake ◽

Akiko Ishii ◽

Naoko Okiyama ◽

Akira Tamaoka

Keyword(s):

Peripheral Neuropathy ◽

Interstitial Pneumonia ◽

Muscle Weakness ◽

Nerve Conduction Study ◽

Nerve Conduction ◽

Skin Lesions ◽

Amyopathic Dermatomyositis ◽

First Report ◽

Controversial Concept

We provide the first report of amyopathic dermatomyositis combined with peripheral neuropathy. Our patient, a 49-year-old woman, initially experienced muscle weakness and tingling sensations in her legs, and nerve conduction study findings and the detection of antiganglioside antibodies indicated that she had autoimmune peripheral neuropathy. The unexpected presence of skin lesions, interstitial pneumonia and antibodies to melanoma differentiation-associated protein 5 prompted an additional diagnosis of amyopathic dermatomyositis. No previous report has described amyopathic dermatomyositis with peripheral neuropathy, and the present case provides evidence for the once-controversial concept of neuromyositis.

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Global Eradication of Yaws: Neglected Disease with Research Priority

Journal of Medicine ◽

10.3329/jom.v10i2.2825 ◽

1970 ◽

Vol 10 (2) ◽

pp. 109-114

Author(s):

Md Robed Amin ◽

Ariful Basher ◽

Md Ferdous Zaman ◽

MA Faiz

Keyword(s):

Treponema Pallidum ◽

Skin Lesions ◽

Point Of View ◽

World Health ◽

Benzyl Penicillin ◽

Neglected Disease ◽

World Health Assembly Resolution ◽

Control Programmes ◽

Global Eradication ◽

Long Acting

Yaws is a disfiguring, debilitating non-venereal treponemal infection caused by Treponema pallidum subspecies pertenue. It is a contagious disease transmitted by direct (person-to-person) contact with the infectious yaws lesion. Early lesions of this disease manifest in the form of skin lesions, which on healing show little scarring. The disease can be progressive wherein bone and cartilage are affected leading to disability. The disease can be cured and prevented by a single injection of long acting (benzathine benzyl) penicillin. Between 1952 and 1964, a worldwide campaign led by WHO and the United Nations Children's Fund (UNICEF) to control and eventually eradicate yaws and other endemic treponematoses were undertaken.By the end of the 1970s, reemergence of yaws occured in many countries and it prompted a World Health Assembly Resolution requesting the implementation of integrated treponematoses control programmes. While the programme initially showed tremendous result but yaws control efforts paved the way for the development of the primary healthcare system in affected areas. The disease is amenable to eradication epidemiologically, technologically, historically and from political point of view if appropriate research based programme in national and international arena is set. Key words: Eradication, Yaws, Neglected,disease,Research doi: 10.3329/jom.v10i2.2825 J MEDICINE 2009; 10 : 109-114

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Diagnosis of Meningococcal Meningitis by Broad-Range Bacterial PCR with Cerebrospinal Fluid

Journal of Clinical Microbiology ◽

10.1128/jcm.36.8.2205-2209.1998 ◽

1998 ◽

Vol 36 (8) ◽

pp. 2205-2209 ◽

Cited By ~ 78

Author(s):

Pirkko Kotilainen ◽

Jari Jalava ◽

Olli Meurman ◽

Olli-Pekka Lehtonen ◽

Esa Rintala ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Bacterial Meningitis ◽

Bacterial Culture ◽

Rrna Genes ◽

23S Rrna ◽

Acute Bacterial Meningitis ◽

Rrna Gene ◽

Gram Staining ◽

Negative Findings ◽

New Research

We used broad-range bacterial PCR combined with DNA sequencing to examine prospectively cerebrospinal fluid (CSF) samples from patients with suspected meningitis. Fifty-six CSF samples from 46 patients were studied during the year 1995. Genes coding for bacterial 16S and/or 23S rRNA genes could be amplified from the CSF samples from five patients with a clinical picture consistent with acute bacterial meningitis. For these patients, the sequenced PCR product shared 98.3 to 100% homology with the Neisseria meningitidis sequence. For one patient, the diagnosis was initially made by PCR alone. Of the remaining 51 CSF samples, for 50 (98.0%) samples the negative PCR findings were in accordance with the negative findings by bacterial culture and Gram staining, as well as with the eventual clinical diagnosis for the patient. However, the PCR test failed to detect the bacterial rRNA gene in one CSF sample, the culture of which yielded Listeria monocytogenes. These results invite new research efforts to be focused on the application of PCR with broad-range bacterial primers to improve the etiologic diagnosis of bacterial meningitis. In a clinical setting, Gram staining and bacterial culture still remain the cornerstones of diagnosis.

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