scholarly journals Influence of microbiological starter of filler of biofilter of "Filtronorm D" on stored of rainbow trout

Author(s):  
N. Grynevych

Creation in biofilters of favorable conditions for the existence of biocenoses secures RAS from the toxic effects of nitrites, which until recently did not attach great importance to toxicants for aquatic organisms. However, it has been found that they are very toxic to fish and aquatic invertebrates. The purpose of the work was to investigate the effect of microbiological starter of the "Filtronorm-D" biofilter filler on the behavior of adult rainbow trout during the launch of the biofilter. Investigation of the influence of the microbial starter of the filtrotorm-D biofilter filler on the behavior and clinical condition and the preservation of rainbow trout during the launch of the biofilter of the ultrasound has been studied. Evaluated the clinical signs of adult rainbow trout during the launch of the biofilter CWS for the use of polypropylene filler RK PLAST-control in it. According to the second variant, the clinical signs of adult rainbow trout were evaluated during the startup of the biofilter of the ultrasound scanner for the use of polypropylene filler RK PLAST in it and the addition of the microbiological starter of the biofilter "Filtronorm-D" – experiment. In the experimental group, the biofilter water was introduced by our microbiological starter, biofilter filler "Filtronorm-D", containing live cultures of nitrifying and denitrifying bacteria in the amount of 107 CFU/cm3. On the 10th day of the experiment, after the application of the microbiological starter of the biofilter filler "Filtronorm-D", approximately 5 % of the fish with the initial clinical signs of nitrite poisoning were detected. The fish for a long time stood motionless in the corners of the pool, they had the darkening of the body and the brown color of the gills. On the 15th day after the launch of the CWS and the use of "Filtronorm-D", changes in behavior were detected in most of the research objects, which may indicate an increase in the amount of nitrites in water and their effect on the body of trout. Approximately 25 % of fish for a long time stays motionless in the corners of the pool, the mobility of the whole fish has decreased, some individuals have risen to the surface of the water. Changes in pigmentation of the body were also found in approximately 10 % of the individuals. The body became darker, the fins were sealed, the gills got brown color. During this trial period, the mortality rate of the fish was 1,3 %. On the 20th day of the experiment, the signs of nitrite poisoning of fish became increasingly pronounced. Approximately 30 % of the fish showed the presence of darkening of the body and fins, the latter were sealed. In the same fish, the changed color of the gills was observed – from red to brown. In addition, during this period of research, the highest trout death was determined – 3,1 %, which is almost 2.4 times (p <0,05) higher than in the 15-day period. The period of ultrasound starting from the 20th to the 25th day was characterized by a decrease in the content of nitrites from 1,1 mg/dm3 to 0.6 mg/dm3. The process of reducing the content of nitrites in water affected the activity of fish – only about 20 % of trout swam in the upper layers of water. On the 30th day of the launch of the ultrasound with the use of the microbiological starter of the biofilter filler "Filtronorm-D" no signs of nitrite poisoning in rainbow trout were noted. The loss of fish in the indicated period was practically not marked. At the same time, during the application of polypropylene filler RK PLAST without the addition of a microbiological starter of the biofilter filler "Filtronorm D", the most problematic period for fish, in which it is most dying due to the effect of nitrites, is the period from 25 to 30 days. Thus, the conducted researches prove that during the launch of ultrasound for the use of polypropylene filler RK PLAST in it and the addition of the microbiological starter of the biofilter fillter "Filtertronorm D" the most dangerous is the period from 15 to 20 days. During this period, the largest death of fish and signs of nitrite poisoning is recorded, which requires the implementation of veterinary and sanitary preventive measures to reduce the toxic effects of nitrites. Key words: RAS, rainbow trout, biofilter, "Filtronorm-D", nitrites, fish behavior, signs of poisoning.

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 105-108
Author(s):  
N.H. Sorokinа ◽  
O.K. Halchynska ◽  
V.S. Lepushynska

Psittacosis – a zooanthroponosis infection. In humans, the disease can manifest atypical pneumonia, enteritis, peritonitis, encephalitis. In parrots the disease starts with loss of appetite, drowsiness, weakness, fever, appearance of conjunctivitis, rhinitis and diarrhea. The causative agent of psittacosis in parrots are obligate intracellular microorganism Chlamydophila psittaci, which enters the body through the epithelium of the mucous membranes of respiratory tract. Its reproduction and accumulation occurs in the epithelial, and lymphoid reticulohistiocytosis cells. Chlamydia capable to long time cellular localization, can penetrate into the various organs and systems: the lungs, the nervous, cardiovascular system, liver. Parrot, who had been ill for psittacosis, is usually long remain carriers of Chlamydia. This article contains the definition of «psittacosis» as the disease, etiology, pathogenesis, main clinical features of parrots, pathological changes, especially the diagnosis, treatment and prevention. This materials are can help to owners of parrot and veterinary professionals to understand the disease, clinical signs, modern methods of diagnosis, prevention and therapy of psittacosis. 


VCOT Open ◽  
2020 ◽  
Vol 03 (01) ◽  
pp. e23-e27
Author(s):  
Massimo Petazzoni

Abstract Objective The aim of this study was to report an unusual case of an iatrogenic foreign body within the stifle joint, removed 6 years after distal femoral fracture repair in a small dog. Acute lameness was caused by a migrating screwdriver fragment, which had been lost during initial fracture repair and which did not cause clinical signs for years until dislodged inside the joint. Case Report A male Jack Russell, 7 years of age, underwent plate and screws fixation to treat a Salter-Harris type I fracture at the age of 9 months. Seven months after surgery, partial implant removal was performed. Six years after fracture repair, the dog presented for a non-weight-bearing lameness and pain on the previously operated stifle joint, which occurred suddenly while walking on the leash. Radiographs revealed a small intra-articular metallic radiodense foreign body. Arthroscopy was used to remove the migrating broken-tip of a screwdriver from the medial aspect of the tibiofemoral joint. Retrieving the foreign body led to the immediate resolution of clinical signs without complications. Conclusion By reporting this case of a forgotten foreign body retained inside a stifle joint for 6 years, we aim to illustrate the potential risk of leaving a piece of metal inside the body. A high index of suspicion for such complications should be kept a long time after surgery. Arthroscopy was a useful first-line tool for efficiently and mini-invasively treating this unusual problem.


2000 ◽  
Vol 19 (1) ◽  
pp. 19-25 ◽  
Author(s):  
King C. Lee ◽  
Li-Mou Zheng ◽  
Xiang Luo ◽  
Caroline Clairmont ◽  
Jessica Fischer ◽  
...  

The objective of these studies was to perform a comparative evaluation of the acute toxicity of VNP20009, a genetically engineered Salmonella strain, in monkeys, pigs, and mice. It is hypothesized that mice would be more susceptible than other animal species to the toxic effects of VNP20009, because mice are the most sensitive natural host for the parental wild-type Salmonella typhimurium strain. These studies also compared the virulence of VNP20009 and the parental Salmonella in mice. In Cynomolgus monkeys and Yorkshire pigs ( n = 2/dose), various doses (expressed as colony forming units [cfu] per animal) of VNP20009, or vehicle, were administered as a single IV injection (∼ 1 ml/min). The body weight, body temperature, clinical signs, clinical pathology (serum chemistry and hematology), and ophthalmic examinations (only in monkeys) were evaluated at various times. Necropsy was performed on day 15 in the pigs, and necropsy and histopathology on days 8 or 15 in the monkeys. In C57BL/6 mice ( n = 10/dose), various doses of VNP20009, or the parental Salmonella, were administered as a single IV bolus injection. The mice were observed daily over 3 weeks. The results from monkeys showed that VNP20009-related changes in clinical pathology were primarily confined to fiver enzymes and fiver function tests (i.e., cholesterol, triglyceride, alanine aminotransferase, and aspartate aminotransferase levels). Significant toxicological changes occurred only at the dose of 1 × 1010 cfu/monkey, but not at the doses of 1 × 108 or 3 × 109 cfu/monkey. Gross necropsy and histology findings were primarily confined to the spleen (enlargement, weight increase, and reticuloendothelial hyperplasia), thymus (size and weight reduction and lymphoid depletion), mesenteric lymph node (enlargement), and lung (weight increase). Most of these necropsy and microscopic findings, which occurred mostly in the high-dose group, may be related to the physiological responses to infection, rather than related to the intrinsic toxicity of VNP20009. The results from pigs showed that VNP20009 induced toxicological effects only at the dose of 3 × 109 cfu/pig, but not at the doses of 3 × 108 or 3 × 1010 cfu/pig. Both pigs treated with 3 × 1010 cfu/pig died within the first 2 days post-treatment. Necropsy showed the presence of abdominal transudate fluid, skin blotching, and pulmonary-and gall bladder-associated edema. Therefore, the pig mortality may have been related to the physical damage induced by the sudden systemic presence of large amounts of suspension. The results from mice showed that VNP20009, at doses as high as 1 × 106 cfu/mouse, did not induce any mortality. A 30% mortality rate was induced by 3 × 106 cfu/mouse, and 100% mortality by 1 × 107 cfu/mouse. The parental Salmonella, at a dose of 1 × 102 or 3 × 102 cfu/mouse, induced a 100% mortality. In conclusion, the doses of VNP20009 that induced acute toxicity are very high, suggesting that VNP20009 may be a safe agent. The virulence is 50,000 × less in VNP20009 than the parental Salmonella.


Author(s):  
A. O. Boiko

It  was made the research of the adaptive ability and natural resistance of Polissia meat breed animals, the area of their content was limited by the conditions of the lowland, forested and bogged territory of the Ukrainian Polissia and Precarpathian. Materials for analysis were selected with scientific publications and works, which reflect the issue of the breeding and maintenance of Polissia meat breed animals, as well as their own research on the natural resistance of cows. As a result of the analysis of the obtained  information, it was found out that the cows of the 1st experimental group of the high-growth type, they  possess a high potential of natural resistance, a somewhat lower resistance was detected in the cows of the intermediate type (II control group) and compact type (III experimental group). From these studies it emerges that there is the advisability of a comprehensive study of the type of constitution, since this determines the level of natural resistance and light resistance of animals. Only strong animals are able to be healthy, to give a viable and full-fledged offspring, to have high productivity, to withstand constant operational loads on the body and for a long time to be rationally operated with the greatest economic efficiency.


Author(s):  
P. О. Zaika ◽  
◽  
O. V. Kantemir ◽  

Dogs play a significant role in human life. It is difficult to list all the various aspects of their application. But along with the positive qualities of using dogs is the problem of regulating the numbers of stray animals. Among the agents that stimulate the processes of repair and regeneration, increase the basic physiological mechanisms of protection of the body are humic preparations such as sodium humate. Taking into account that preparations of natural origin have a special influence on the inflammatory process, it is necessary to find and study the possibilities of using biological preparations of humic acids. Six animals were selected for the detection of the effect of sodium humate on dogs by analogues. Castration was carried out in an open manner according to conventional methods After surgery, the animals were subcutaneously injected with a 0.1% solution of sodium humate at a dose of 0.1 ml per 1 kg of body weight, and the control saline solution once a day for 14 days. Examination of wounds and thermometry was performed twice daily for 16 days. Castration wounds were treated with antibacterial aerosol once daily. No additional medication was used. In the post-castration period, all operated dogs did not observe any bleeding, loss of the common vaginal sheath, intestine, cult of the seminal cord, or other complications. Since the third day in the general condition of the animals of the experimental group significant changes were observed, which were expressed in the attenuation of the signs of inflammation, which was manifested in the reduction of hyperemia of the wound edges and their edema. The tenderness of the scrotal tissue was noted, and the scrotal skin became folded. In control animals the development of the inflammatory process was more pronounced and occurred 2 to 3 days later than in the experimental animals. On the third day after surgery, the control animals showed clinical signs characteristic of acute inflammation: hyperemia, sharp pain and swelling of the scrotal tissues, elevated local temperature. In two animals of the control group swelling was spread beyond the scrotum - a slight swelling of the prepuce was noted. These signs were manifested against the background of general depression and decreased appetite. Recovery occurred on day 12 in the experimental group and on day 16 in the control one.


2008 ◽  
Vol 53 (No. 6) ◽  
pp. 324-332 ◽  
Author(s):  
J. Velisek ◽  
Z. Svobodova ◽  
V. Piackova ◽  
L. Novotny ◽  
J. Blahova ◽  
...  

The aim of this study was to assess the effect of metribuzin on rainbow trout (<I>Oncorhynchus mykiss</I>). An experimental group of fish was exposed to Sencor 70 WG pesticide product (active substance 70% of metribuzin). The acute semistatical toxicity test lasting 96 h was performed on rainbow trout juveniles. The 96hLC50 value of Sencor 70 WG was 89.3 mg/l. An examination of the haematological and biochemical profile and histopathological tissue examinations were performed on one- to two-year-old rainbow trout after 96 h of exposure to Sencor WG 70 in a concentration of 89.3 mg/l. The experimental group showed significantly lower values (<I>P</I> < 0.01) of plasma total proteins, triacylglycerols, aspartate aminotransferase, ammonia, calcium, lactate, alkaline phosphatase, erythrocyte count, haematocrit and significantly higher (<I>P</I> < 0.01) values of erythrocyte haemoglobin compared to the control group. A significant decrease (<I>P</I> < 0.01) in both the relative and absolute lymphocyte count and a significant increase (<I>P</I> < 0.01) in both the relative and absolute count of neutrophile granulocytes were also recorded in the experimental group. The histopathological examination revealed mild proliferation of goblet cells of the respiratory epithelium of secondary gill lamellae and hyaline degeneration of epithelial cells of the renal tubules of the caudal kidney. This alteration of kidney resulted in hypoproteinaemia, followed by the formation of transudate in the body cavity. The metribuzin-based Sencor WG 70 pesticide product was classified among substances harmful to fish.


Author(s):  
P. Vered ◽  
V. Bityutsky ◽  
V. Kharchyshyn ◽  
M. Zlochevskiy

Generalized studies of the world scientific literature on the fate and risk assessment of exposure to silver nanoparticles (NPAg) both at the ecosystem level and at the organism level, as well as in the laboratory. It is emphasized that the toxic effect of silver nanoparticles, mechanisms and methods of action of NPAg on the body of aquatic organisms have been sufficiently studied in laboratory practice. However, there are some gaps and discrepancies between the results of laboratory tests and the study of real environmental consequences, and such inconsistencies hinder the development of appropriate effective measures to achieve environmental well-being. To bridge such gaps, this review summarizes how environmental conditions and the physicochemical properties of NPAg influence conflicting conclusions between laboratory and real-world environmental studies. It is emphasized that modern research on the pathways of entry, transformation and bioaccumulation of silver nanoparticles in natural aquatic ecosystems emphasizes the ability of such nanoparticles to penetrate intact physiological barriers, which is extremely dangerous. It is proved that silver nanoparticles have a toxic effect on microorganisms, macrophytes and aquatic organisms. The toxic effects of NPAg cover almost entire aquatic ecosystems. A study by a number of authors on the factors influencing the mobility, bioavailability, toxicity and environmental fate of Ag nanoparticles was analyzed to assess the environmental risk. In addition, this review systematically examines the various toxic effects of silver nanoparticles in the environment and compares these effects with the results obtained in laboratory practice, which is useful for assessing the environmental effects of such compounds. The dangerous chronic effects of low-concentration NPAg (μg/l) on natural aquatic ecosystems over a long period of time (months to several years) have been described in detail. In addition, the prospects for future studies of NPAg toxicity in natural freshwater environments are emphasized. Key words: nanoparticles of the medium (NPAg), ecosystem, laboratory wash, toxicity, aquatic organisms, ecological factors.


2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.


Author(s):  
M. Grigoryev ◽  
N. Chernogradskaya

Animals imported to Yakutia from other regions fall into feeding and housing conditions that differ from their usual agro-climatic conditions, which causes the problem of their adaptation. Yakutia is one of the areas where the feed of its own production has a lack of basic minerals, which affects the health and productivity of animals, which has an additional negative influence on the adaptation process of imported cattle. In order to replenish macro- and microelements in the body, it is necessary to introduce various local mineral feed additives, premixes, and use mineral salts into the ration. Under the conditions of Central Yakutia the influence of local mineral feed additives (zeolite of the Khonguruu Deposit in the Suntar district and sapropels of local lakes) containing macro- and microelements that increase the meat productivity of steers has been studied. As a result of the research the influence of local mineral feed additives on meat productivity and physiological condition of Hereford steers has been established. Influence of zeolite and sapropel in a complex with mineral salts on digestibility of nutrients of the ration and use of nitrogen, calcium and phosphorus by experimental animals has been defined. The influence of feed additives on the quality of meat products has been studied. The results have shown that the use of local mineral feed additives in the composition of ration of steers during the fattening period at a dose of 0,7 g zeolite per 1 kg of live weight of the animal and 200 g of sapropel in combination with 10 g of mineral salts contributed to the gain in live weight, carcass weight, carcass yield, yield of edible parts, and economic efficiency of fattening. For the entire period of the experiment the gain in live weight in groups was: in control group 2222,9 kg, in the 1st experimental group 2396,2 kg, in the 2nd experimental group 2471,1 kg. The total economic effect for the 1st experimental group for the period of experiment amounted to 44,3 thousand Rubles, for the 2nd experimental group 78,0 thousand Rubles or per 1 head/day 14,06 and 24,76 Rubles, respectively.


Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.


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