scholarly journals Extraction and Characterization of Secondary Metabolites from Medicinal Plants

2020 ◽  
Vol 2 (1) ◽  
pp. 87
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Antioxidant Property ◽  
Cassia Alata ◽  
Thespesia Populnea ◽  
Solvent Extraction Method ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
Ancient Times ◽  
Wrightia Tinctoria

Most of the current medicines at use are derived from phyto-compounds. Plant-based products are used since ancient times to cure various ailments. Due to modern civilization, the properties of numerous medicinal plants remain undiscovered or dormant. This study deals with Cassia alata, Thespesia populnea, and Wrightia tinctoria. The extracts Cassia alata, Thespesia populnea, and Wrightia tinctoria were checked for their bioactive properties. Secondary metabolites such as alkaloids and saponins were withdrawn from dried leaves using the solvent extraction method. The secondary metabolites were characterized by UV-Vis spectroscopy (UV-Vis) and Fourier Transform Infrared Spectroscopy (FT-IR). The metabolites were evaluated for an antioxidant property using DPPH. The metabolites were further checked for larvicidal activity.

Green Synthesis of Silver Nanoparticles Using Olden-landia corymbosa and Ocimum sanctum Leaf Aqueous Extract

2020 ◽  
Vol 13 (3) ◽  
pp. 4959-4964
Author(s):  
Prof.(Dr.) Sirshendu Chatterjee Chatterjee ◽  
Shaktijit Das ◽  
Moumita Saha ◽  
Pranabesh Ghosh ◽  
Titav Sengupta ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Nano Particles ◽  
Bioactive Molecules ◽  
Primary Amines ◽  
Ocimum Sanctum ◽  
Leaf Aqueous Extract ◽  
Vis Spectroscopy ◽  
Ft Ir

The field of nanotechnology is the most renowned area of research in modern day science. One of the most convenient and comprehensive technique related to the synthesis of nanoparticles using plant extracts and their subsequent action. On the other hand, herbal medicines become an integral part of modern-day treatment strategy around the globe; combination of these two ways are widely accepted and beneficial due to its low cost, better societal recognition and less side effects. Phytochemical analysis revealed that leaf aqueous extract of two medicinal plants Oldenlandia corymbosa and Ocimum sanctum, harbours high concentration of antioxidants and bioactive molecules. Hence our present course of study aims at the green synthesis of silver nano-conjugates using Oldenlandia corymbosa and Ocimum sanctum leaf aqueous extracts. The synthesized nano-conjugates have undergone advanced chara-cterization techniques including UV-Vis Spectro-photometry, DLS, and FT-IR. The UV-Vis spectroscopy results showed single peak at 420 nm and 430 nm for Oldenlandia corymbosa and Ocimum sanctum nano-conjugates, respectively. The particle size for Oldenlandia corymbosa and Ocimum sanctum nano-particles are 21.04 nm and 24.36 nm, respectively as observed from the DLS data, and finally, the FT-IR data confirms the conjugation of bioactive molecules of the medicinal plants indicating that primary amines, secondary amines, alcohols, phenols, aldehydes, aliphatic amines, alkenes, functional groups of the bioactive molecules are responsible for the stability of prepared silver nano-conjugates

scholarly journals By-product hydrosol of Eucalyptus globulus essential oil distillation as source of botanical insecticides: wealth from waste

2021 ◽  
Vol 13 (1) ◽  
pp. 10854
Author(s):  
Arun D. SHARMA ◽  
Inderjeet KAUR
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Essential Oil ◽  
Human Health ◽  
Insecticidal Activity ◽  
Eucalyptus Globulus ◽  
Botanical Insecticides ◽  
Ft Ir ◽  
Mealy Bug ◽  
Chemical Pesticides

Worldwide use of chemical pesticides is on rise which is creating a big problem to environment and human health. Hence great interest has been generated to find out botanical leads having anti-pest properties from medicinal plants. The objective of this work was to evaluate the insecticidal potential of Eucalyptus globulus waste hydrosol obtained after essential oil distillation. Samples of Eucalyptus globulus genus were collected from near-by areas of study, and were hydro-distilled and their by-product ‘hydrosol was analyzed for phenolics and tannin determination. UV-VIS, FT-IR and fluorescent study was also conducted of by-product hydrosol. In addition, insecticidal activity of by-product hydrosol was also monitored against mealy bug. Substantial amount of phenolics and tannins were detected in the by-product hydrosol. UV and fluorescent spectroscopy revealed the presence of secondary metabolites. Significantly higher insecticidal activity was observed of by-product hydrosol against mealy bug. The results suggested that by-product hydrosol from Eucalyptus globulus essential oil distillation can be considered as potential candidates for bio-control of pests.

scholarly journals Skrining Fitokimia, Uji Toksisitas dan Uji Peredaman Radikal DPPH Ekstrak Daun Gelinggang (Cassia alata L)

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 61-66
Author(s):  
Izkia Mawaddah ◽  
Erwin E ◽  
Chairul Saleh
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Skin Diseases ◽  
Antioxidant Properties ◽  
Artemia Salina ◽  
Cassia Alata

Cassia alata L. (Gelinggang) one of the plants known as medicinal plants, especially for the treatment of skin diseases. The purpose of this study was to determine the type of secondary metabolites using phytochemical tests, toxicity with the BSLT method and antioxidant properties against DPPH radicals from the extract of the Gelinggang leaves. The results of the study showed extracts of Gelinggang leaves containing alkaloids, triterpenoids, steroids, and quinones. Based on the results of toxicity and antioxidant tests showed that Gelinggang leaves extract was very toxic to Artemia salina shrimp and it was a strong antioxidant against DPPH radicals with LC50 and IC50 were 28.84 ppm and 73.57 ppm, respectively. Keywords: Gelinggang, Cassia alata L., toxicity, antioxidants, DPPH

scholarly journals Phytochemical Properties and Pharmacological Role of Plants: Secondary Metabolites

2021 ◽  
Vol 18 (1) ◽  
pp. 23-35
Author(s):  
Bhupesh Kaushik ◽  
Jatin Sharma ◽  
Keshav Yadav ◽  
Prithik Kumar ◽  
Abhilasha Shourie
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Human Health ◽  
Herbal Medicines ◽  
Biotic And Abiotic Stress ◽  
Pharmacological Activities ◽  
Anti Cancer ◽  
Human Disorders ◽  
Ancient Times ◽  
Bioactive Phytochemicals

Over the past decades, there has been increasing attention tothe study of medicinal plants that contain many phytochemicals beneficial for human health. A number of secondary metabolites derived from various plants have been used as drug components to treat several human disorders since ancient times. The traditional therapeutic applications of secondary metabolites have been reported in the whole world. Numerous bioactive phytochemicals constituents have been identified and isolated using many advanced techniques. These bioactive phytochemicals are responsible for many pharmacological activities such as anti-inflammation, anti-cancer, anti-allergic, and antimicrobial infection. These secondary metabolites are not only beneficial for human health but also protect plants themselves from biotic and abiotic stress. These secondary metabolites are classified into many subclasses like terpenoids, alkaloids and phenolics. Each class of secondary metabolites has its pharmacological activities, which is required to be studied thoroughly for better use in pharmaceuticals, cosmetics, food, and other industries. Therefore, this review paper represents many medicinal plants that contain bioactive secondary metabolites and show pharmacological activities, which provides an opportunity to utilize them for improvement of human health and discover new herbal medicines.

LegumeDB: Development of Legume Medicinal Plant Database and Comparative Molecular Evolutionary Analysis of matK Proteins of Legumes and Mangroves

2019 ◽  
Vol 15 (4) ◽  
pp. 353-362
Author(s):  
Sambhaji B. Thakar ◽  
Maruti J. Dhanavade ◽  
Kailas D. Sonawane
Keyword(s):  
Phylogenetic Analysis ◽  
Medicinal Plants ◽  
Homology Modeling ◽  
Sequence Alignment ◽  
Vigna Unguiculata ◽  
Multiple Sequence Alignment ◽  
Legume Species ◽  
Mangrove Species ◽  
Multiple Sequence ◽  
Thespesia Populnea

Background: Legume plants are known for their rich medicinal and nutritional values. Large amount of medicinal information of various legume plants have been dispersed in the form of text. Objective: It is essential to design and construct a legume medicinal plants database, which integrate respective classes of legumes and include knowledge regarding medicinal applications along with their protein/enzyme sequences. Methods: The design and development of Legume Medicinal Plants Database (LegumeDB) has been done by using Microsoft Structure Query Language Server 2017. DBMS was used as back end and ASP.Net was used to lay out front end operations. VB.Net was used as arranged program for coding. Multiple sequence alignment, phylogenetic analysis and homology modeling techniques were also used. Results: This database includes information of 50 Legume medicinal species, which might be helpful to explore the information for researchers. Further, maturase K (matK) protein sequences of legumes and mangroves were retrieved from NCBI for multiple sequence alignment and phylogenetic analysis to understand evolutionary lineage between legumes and mangroves. Homology modeling technique was used to determine three-dimensional structure of matK from Legume species i.e. Vigna unguiculata using matK of mangrove species, Thespesia populnea as a template. The matK sequence analysis results indicate the conserved residues among legume and mangrove species. Conclusion: Phylogenetic analysis revealed closeness between legume species Vigna unguiculata and mangrove species Thespesia populnea to each other, indicating their similarity and origin from common ancestor. Thus, these studies might be helpful to understand evolutionary relationship between legumes and mangroves. : LegumeDB availability: http://legumedatabase.co.in

scholarly journals Efficacy Assessment of Biosynthesized Copper Oxide Nanoparticles (CuO-NPs) on Stored Grain Insects and Their Impacts on Morphological and Physiological Traits of Wheat (Triticum aestivum L.) Plant

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 233
Author(s):  
Ali A. Badawy ◽  
Nilly A. H. Abdelfattah ◽  
Salem S. Salem ◽  
Mohamed F. Awad ◽  
Amr Fouda
Keyword(s):  
Block Design ◽  
Dry Weight ◽  
Triticum Aestivum L ◽  
Copper Oxide Nanoparticles ◽  
Rhyzopertha Dominica ◽  
Stored Grain ◽  
Stabilizing Agents ◽  
Cuo Nps ◽  
Vis Spectroscopy ◽  
Ft Ir

Herein, CuO-NPs were fabricated by harnessing metabolites of Aspergillus niger strain (G3-1) and characterized using UV–vis spectroscopy, XRD, TEM, SEM-EDX, FT-IR, and XPS. Spherical, crystallographic CuO-NPs were synthesized in sizes ranging from 14.0 to 47.4 nm, as indicated by TEM and XRD. EDX and XPS confirmed the presence of Cu and O with weight percentages of 62.96% and 22.93%, respectively, at varied bending energies. FT-IR spectra identified functional groups of metabolites that could act as reducing, capping, and stabilizing agents to the CuO-NPs. The insecticidal activity of CuO-NPs against wheat grain insects Sitophilus granarius and Rhyzopertha dominica was dose- and time-dependent. The mortality percentages due to NP treatment were 55–94.4% (S. granarius) and 70–90% (R. dominica). A botanical experiment was done in a randomized block design. Low CuO-NP concentration (50 ppm) caused significant increases in growth characteristics (shoot and root length, fresh and dry weight of shoot and root, and leaves number), photosynthetic pigments (total chlorophylls and carotenoids), and antioxidant enzymes of wheat plants. There was no significant change in carbohydrate or protein content. The use of CuO-NPs is a promising tool to control grain insects and enhance wheat growth performance.

scholarly journals Synthesis, Fabrication, and Characterization of Functionalized Polydiacetylene Containing Cellulose Nanofibrous Composites for Colorimetric Sensing of Organophosphate Compounds

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1869
Author(s):  
A K M Mashud Alam ◽  
Donovan Jenks ◽  
George A. Kraus ◽  
Chunhui Xiang
Keyword(s):  
Solid State ◽  
Hydroxyl Groups ◽  
Colorimetric Sensing ◽  
Electrospinning Technique ◽  
Hazardous Chemical ◽  
Tensile Tester ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
Landmark Study

Organophosphate (OP) compounds, a family of highly hazardous chemical compounds included in nerve agents and pesticides, have been linked to more than 250,000 annual deaths connected to various chronic diseases. However, a solid-state sensing system that is able to be integrated into a clothing system is rare in the literature. This study aims to develop a nanofiber-based solid-state polymeric material as a soft sensor to detect OP compounds present in the environment. Esters of polydiacetylene were synthesized and incorporated into a cellulose acetate nanocomposite fibrous assembly developed with an electrospinning technique, which was then hydrolyzed to generate more hydroxyl groups for OP binding. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), Instron® tensile tester, contact angle analyzer, and UV–Vis spectroscopy were employed for characterizations. Upon hydrolysis, polydiacetylene esters in the cellulosic fiber matrix were found unaffected by hydrolysis treatment, which made the composites suitable for OP sensing. Furthermore, the nanofibrous (NF) composites exhibited tensile properties suitable to be used as a textile material. Finally, the NF composites exhibited colorimetric sensing of OP, which is visible to the naked eye. This research is a landmark study toward the development of OP sensing in a protective clothing system.

scholarly journals Synthesis, Biomacromolecular Interactions, Photodynamic NO Releasing and Cellular Imaging of Two [RuCl(qn)(Lbpy)(NO)]X Complexes

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2545
Author(s):  
Luna Song ◽  
Hehe Bai ◽  
Chenyang Liu ◽  
Wenjun Gong ◽  
Ai Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Confocal Imaging ◽  
Epr Spectra ◽  
Distorted Octahedral Geometry ◽  
Ionization Mass ◽  
Time Resolved ◽  
Pbr322 Dna ◽  
Vis Spectroscopy ◽  
No Release ◽  
Ft Ir

Two light-activated NO donors [RuCl(qn)(Lbpy)(NO)]X with 8-hydroxyquinoline (qn) and 2,2′-bipyridine derivatives (Lbpy) as co-ligands were synthesized (Lbpy1 = 4,4′-dicarboxyl-2,2′-dipyridine, X = Cl− and Lbpy2 = 4,4′-dimethoxycarbonyl-2,2′-dipyridine, X = NO3−), and characterized using ultraviolet–visible (UV-vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (1H NMR), elemental analysis and electrospray ionization mass spectrometry (ESI-MS) spectra. The [RuCl(qn)(Lbpy2)(NO)]NO3 complex was crystallized and exhibited distorted octahedral geometry, in which the Ru–N(O) bond length was 1.752(6) Å and the Ru–N–O angle was 177.6(6)°. Time-resolved FT-IR and electron paramagnetic resonance (EPR) spectra were used to confirm the photoactivated NO release of the complexes. The binding constant (Kb) of two complexes with human serum albumin (HSA) and DNA were quantitatively evaluated using fluorescence spectroscopy, Ru-Lbpy1 (Kb~106 with HSA and ~104 with DNA) had higher affinity than Ru-Lbpy2. The interactions between the complexes and HSA were investigated using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and EPR spectra. HSA can be used as a carrier to facilitate the release of NO from the complexes upon photoirradiation. The confocal imaging of photo-induced NO release in living cells was successfully observed with a fluorescent NO probe. Moreover, the photocleavage of pBR322 DNA for the complexes and the effect of different Lbpy substituted groups in the complexes on their reactivity were analyzed.

scholarly journals Synthesis of Poly(methacrylic acid-co-butyl acrylate) Grafted onto Functionalized Carbon Nanotube Nanocomposites for Drug Delivery

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 533 ◽  
Author(s):  
Josué A. Torres-Ávalos ◽  
Leonardo R. Cajero-Zul ◽  
Milton Vázquez-Lepe ◽  
Fernando A. López-Dellamary ◽  
Antonio Martínez-Richa ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Drug Delivery ◽  
Methacrylic Acid ◽  
Butyl Acrylate ◽  
Chemical Vapor ◽  
Vis Spectroscopy ◽  
Ir And Raman Spectroscopies ◽  
Ft Ir

Design of a smart drug delivery system is a topic of current interest. Under this perspective, polymer nanocomposites (PNs) of butyl acrylate (BA), methacrylic acid (MAA), and functionalized carbon nanotubes (CNTsf) were synthesized by in situ emulsion polymerization (IEP). Carbon nanotubes were synthesized by chemical vapor deposition (CVD) and purified with steam. Purified CNTs were analyzed by FE-SEM and HR-TEM. CNTsf contain acyl chloride groups attached to their surface. Purified and functionalized CNTs were studied by FT-IR and Raman spectroscopies. The synthesized nanocomposites were studied by XPS, 13C-NMR, and DSC. Anhydride groups link CNTsf to MAA–BA polymeric chains. The potentiality of the prepared nanocomposites, and of their pure polymer matrices to deliver hydrocortisone, was evaluated in vitro by UV–VIS spectroscopy. The relationship between the chemical structure of the synthesized nanocomposites, or their pure polymeric matrices, and their ability to release hydrocortisone was studied by FT-IR spectroscopy. The hydrocortisone release profile of some of the studied nanocomposites is driven by a change in the inter-associated to self-associated hydrogen bonds balance. The CNTsf used to prepare the studied nanocomposites act as hydrocortisone reservoirs.

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