scholarly journals Topical Effect of Minoxidil Containing Lotion on Morphofunctional Indicators of Male Rats’ Reproductive System

2020 ◽  
Vol 89 (2) ◽  
pp. 27-31
Author(s):  
V.A. Turkina ◽  
N.Ye. Chemodurova ◽  
O.I. Grushka ◽  
H.V. Pryzyhlei
Keyword(s):  
Reproductive System ◽  
Epidemiological Studies ◽  
Reproductive Age ◽  
Male Rats ◽  
Experimental Conditions ◽  
Over The Counter ◽  
Depth Study ◽  
Methods Analytical ◽  
Experimental Group

The problem of alopecia, for treatment of which the use of over the counter lotions containing minoxidil is recommended, is paid considerable attention all over the world. The consumers rarely seek medical advice due to certain psychological barriers. Thus, it contributes to the uncontrolled use of the drugs, and fixation of their side effects becomes impossible. There are isolated reports on the effect of minoxidil containing lotions on male fertility. The Aim. To establish organometric parameters of testes and their paradidymis, functional parameters of spermatozoa in mature rats under topical exposure to a 10% minoxidil containing lotion. Methods. Analytical, toxicological, statistical. Results. It was established that under the conditions of 72-day percutaneous application of 10% minoxidil containing lotion some changes in the reproductive system organs can be observed, which are in particular a decrease in the mass of testes and epididymis, as well as a decrease in organs’ linear size. The total time of motor activity of gametes after exposure to a lotion containing 10% minoxidil has statistically significantly shortened, a 6-fold inhibition of the activity of redox processes occurred in the rats of the experimental group compared to the control one. Conclusions. Taking into consideration the obtained data and the uncontrolled and long-term use of 10% minoxidil containing lotions by men of reproductive age, an in-depth study of its gonadotoxic effects in experimental conditions in combination with epidemiological studies of the patients using these lotions is deemed necessary. Key Words: minoxidil, gonadotoxicity, mature rats.

scholarly journals Long-Term Consequences of the Fetoplacental Insufficiency Influence on the State and Functioning of Different Body Systems in Descendants (Literature Review and Own Research)

2020 ◽  
Vol 5 (6) ◽  
pp. 362-369
Author(s):  
N. Yu. Seliukova ◽  
◽  
Keyword(s):  
Literature Review ◽  
Reproductive System ◽  
The State ◽  
Placental Insufficiency ◽  
Reproductive Age ◽  
World Health ◽  
Elastic Fibers ◽  
Pathological State ◽  
The World

The literature review presents the results of processing literature data on one of the topical issues of obstetrics and reproductive medicine - placental insufficiency. According to the World Health Organization, the number of infertile couples is gradually increasing in the world every year. In Ukraine, the frequency of infertile marriages among persons of reproductive age ranges from 12 to 18%. The state of the reproductive system of an adult female is influenced by many factors, from embryonic development to the lifestyle of an adult woman. With an inadequate effect of harmful factors on the fetus, the fetoplacental complex may not react properly, and this is how the symptom complex of disorders arises both on the part of the mother and on the part of the fetus, which is called placental insufficiency, it negatively affects the condition and quality of pregnancy and childbirth. Numerous studies have proven the existence of a connection between the effect of certain factors during pregnancy on the state and functionality of various body systems already in adulthood. It was shown that children who were born from mothers with placental insufficiency suffering from cardiovascular diseases have fewer elastic fibers in the arteries, nephrons in the kidneys. In addition, the pancreas has fewer insulin-producing β-cells and reduced vascularization, as well as altered structure and maturation of the brain, lungs (respiratory distress syndrome) and liver, joint dysplasia, and an imbalance of the immune system. When studying the long-term effects of placental insufficiency, various disorders of physical and mental development are observed, as well as increased somatic and infectious morbidity of newborns and children in the first year of life, in the future it may be the cause of the development of diseases such as arterial hypertension, diabetes mellitus, metabolic syndrome, etc. Conclusion. Our studies have shown the negative impact of placental insufficiency on the reproductive system of adult offspring of rats of two sexes who were born to mothers of two age groups. In particular, the level of testosterone in female offspring was increased against the background of the altered structure of the estrous cycle and the pathological state of ovarian histology. In the male offspring, on the contrary, the total testosterone level decreased, but the histology of the testes and the functional state of the sperm remained unchanged

Long Term Effects of Dibromochloropropane (DBCP) on Male Rats' Reproductive System

Andrologia ◽  
2009 ◽  
Vol 20 (1) ◽  
pp. 60-66 ◽  
Author(s):  
U.A. SOD-MORIAH ◽  
U. SROR ◽  
D. SHEMI ◽  
G. POTASHNIK ◽  
R. CHAYOTH ◽  
...  
Keyword(s):  
Reproductive System ◽  
Male Rats ◽  
Long Term Effects

scholarly journals Effects of Provinols on Cardiodynamics and Coronary Flow in Islodated Rat Hearts

2016 ◽  
Vol 17 (2) ◽  
pp. 99-106
Author(s):  
Ana Popovic ◽  
Olga Pechanova ◽  
Radoslava Rehakova ◽  
Vladimir Zivkovic ◽  
Ivan Srejovic ◽  
...  
Keyword(s):  
Red Wine ◽  
Epidemiological Studies ◽  
Albino Rats ◽  
Alcohol Content ◽  
Experimental Conditions ◽  
Positive Effects ◽  
Rat Hearts ◽  
Wide Range ◽  
Wistar Albino Rats ◽  
Experimental Group

Abstract Provinols are an alcohol-free extract of red wine that contains a wide range of polyphenols. Polyphenols are a group of chemical compounds found in diverse plants. Polyphenols are considered to protect against cardiovascular disease. Although some older epidemiological studies have indicated that the positive effects of red wine on heart disease can be attributed to the alcohol content alone, there is now powerful evidence that polyphenols present in red wine are responsible for these positive effects. The hearts of male Wistar albino rats (n = 36, 12 in each experimental group, 10 weeks old, body mass 250 ± 30 g) were excised and retrogradely perfused according to the Langendorff technique at a gradually increasing perfusion pressure (40-120 cmH2O). Parameters of cardiac function (dp/dt max, dp/dt min, SLVP, DLVP, HR, CF) were measured after perfusion with three different concentrations of provinols (5 μg/ml, 10 μg/ml and 50 μg/ml). Administration of the highest dose (50 μg/ml) induced a significant increase in dp/dt max, dp/dt min, HR and CF compared with control conditions at CPP = 40 cmH2O, while an intermediate dose increased dp/dt max at the same CPP. Generally viewed, the results of the present study suggest that provinols may have a beneficial effect on the intact myocardium and coronary circulation. These findings could constitute an important step in further investigation of these polyphenols under different representative experimental conditions in the heart, as well as providing a good basis for potential clinical studies in this field.

scholarly journals Long-term Effect of Water Pollutants (Heavy Metals) on the Morphofunctional State of the Adult Rat's Pituitary Gland

2021 ◽  
Vol 6 (4) ◽  
pp. 172-178
Author(s):  
N. B. Hryntsova ◽  
◽  
A. M. Romanyuk
Keyword(s):  
Heavy Metals ◽  
Drinking Water ◽  
Pituitary Gland ◽  
Adult Male ◽  
The Body ◽  
Male Rats ◽  
Structural Components ◽  
Experimental Animals ◽  
Experimental Group

In some northern regions of Ukraine there is an increased accumulation of salts of heavy metals in soil and drinking water, which negatively affect the health of the population and become a risk factor for many diseases. The role of the pituitary gland in the regulation of functional disorders of hormonal homeostasis in various pathological conditions is extremely important. The purpose of the study is to study the morphological, morphometric and biochemical rearrangements of the structural components of the adenohypophysis of adult male rats under conditions of long-term exposure to salts of heavy metals. Materials and methods. The experiment was performed on 24 white adult male rats, which were divided into 1 control and 1 experimental group. The experimental group consisted of rats, which for 90 days consumed ordinary drinking water, saturated with a combination of salts of heavy metals: zinc (ZnSO4 · 7H2O) – 5 mg/l, copper (CuSO4 · 5H2O) – 1 mg/l, iron (FeSO4) – 10 mg/l, manganese (MnSO4 · 5H2O) – 0.1 mg/l, lead (Pb (NO3) 2) – 0.1 mg/l and chromium (K2Cr2O7) – 0.1 mg/l. Results and discussion. Long-term effects on the body of experimental animals of salts of heavy metals caused a pronounced compensatory-adaptive changes in the pituitary gland of experimental animals of hypoxic nature. Morphological changes in the organ were accompanied by plethora of venous bed, deterioration of rheological properties of blood, increase in the area of connective tissue component of the gland, violation of histoarchitectonics of epithelial trabeculae, edema in the parenchyma, increase in linear indicators of the pituitary gland. Toxic effects of salts of heavy metals negatively affected the cellular composition of the adenohypophysis: there was an increase in the number of chromophobes and a decrease in the number of chromophilic acidophiles and basophils compared with control animals. At the same time, the increase in linear parameters, area of nuclei and cytoplasm of adenocytes can be attributed both to their compensatory hypertrophy and due to edema processes in the gland caused by deepening hypoxic effects and impaired blood flow from the organ as a result of venous plethora. Conclusion. Morphometric and biochemical parameters of the structural components of the anterior lobe of the adenohypophysis at 90 days of exposure to salts of heavy metals, of course, indicate a significant stress of adaptive processes by the adenohypophysis in response to stressors (salts of heavy metals)

scholarly journals Long-term effects of electromagnetic radiation of the mobile phone frequency on the morphofunctional state of the reproductive system of rats and males and their offspring

2019 ◽  
Vol 63 (2) ◽  
pp. 198-206
Author(s):  
N. V. Chueshova ◽  
F. I. Vismont
Keyword(s):  
Electromagnetic Radiation ◽  
Mobile Phone ◽  
Reproductive System ◽  
Prolonged Exposure ◽  
Significant Inhibition ◽  
Male Rats ◽  
Long Term Effects ◽  
Initial Stage ◽  
Spermatogenic Epithelium

The experiment established the long-term effects of low-intensity electromagnetic radiation from a mobile phone (EMR MP 1745 MHz, xpd = 7.5 ± 0.3 μW/cm2) in the offspring of male rats from parents, one of which was either subjected to a prolonged exposure of the investigated factor. A change in the sex ratio of the born offspring and a decrease in their body weight have been established. The analysis of the reproductive system of the offspring of males when they reached the age of 2 and 4 months, revealed significant impairments, with the most pronounced changes found in the offspring obtained from both the irradiated parents or when only the male was irradiated. These changes are manifested in the disruption of the normal functioning of the spermatogenic epithelium, namely, an intensification of the initial stage of spermatogenesis is noted, with a significant inhibition at the stage of spermatid transformation. A decrease in the number of mature germ cells – spermatozoa and a pronounced deterioration of their viability, as well as an increase in testosterone secretion is established. The revealed violations in the morphofunctional state of the reproductive system of the offspring suggest that the trans-generation effect of EMR exposure generated by cellular sources can be considered as a factor affecting the decline in the male fertility.

scholarly journals Influence of Fetoplacentary Insufficiency of Mothers on the State of Spermatogenesis of Male Offspring

2020 ◽  
Vol 5 (5) ◽  
pp. 343-348
Author(s):  
N. Yu. Seliukova ◽  
◽  
K. V. Misyura ◽  
D. V. Morozenko ◽  
R. V. Dotsenko ◽  
...  
Keyword(s):  
Sex Hormones ◽  
Adult Male ◽  
Reproductive System ◽  
The State ◽  
Male Offspring ◽  
Reproductive Age ◽  
Toxic Substances ◽  
Long Term Effects ◽  
Testosterone Levels

The demographic situation in most countries of the European region, which includes Ukraine, is characterized as quite complex. Nowadays the question of the long-term effects of maternal fetoplacental insufficiency on the functioning of human body systems, in particular on the reproductive system of male offspring, remains open. It is known that negative factors during pregnancy can affect the development and existence of the individual. The purpose of the work was to study the long-term effects of fetoplacental insufficiency on the functioning of the reproductive system of adult male offspring born by mothers of different ages. Material and methods. The study was performed on healthy adult female Wistar rats, young (3 months) and mature (10 months) of reproductive age. 4 groups were formed: the 1st and the 2nd groups included intact animals of young and mature age; the 3rd and the 4th had females with experimental fetoplacental insufficiency of young and mature reproductive age. Modeling of fetoplacental insufficiency was performed by daily subcutaneous injection to females from the 12th to the 18th day of pregnancy 50% oil solution of carbon tetrachloride at a dose of 2 ml/kg body weight. We studied the state of spermatogenesis, weight of internal organs, sex hormones in mature male offspring of 3 months of age after decapitation. Results and discussion. Fetoplacental insufficiency leads to lower testosterone levels in all offspring born to mothers of different reproductive ages. The total level of estradiol remained almost unchanged, but still, in animals there was a shift in the ratio of sex hormones in the direction of hyperestrogenism. Experimental fetoplacental insufficiency in females of different reproductive ages also affected the mass of the testes, epididymis and adrenal glands in their male offspring. According to the indicators of the functional state of epididymal sperm in animals born by reproductively young females, the share of pathological forms of sperm decreased by 45%, in the offspring born by reproductively mature females with fetoplacental insufficiency decreased the number of motile sperm by 46% compared to the intact group of animals. Conclusion. The penetration of toxic substances into the mother's body leads to varying degrees of total xenobiotic load, followed by induction of neutralization reactions and the development of metabolic forms of fetoplacental insufficiency, changing the function of the endocrine system and causing adverse effects on the reproductive system. Fetoplacental insufficiency affects the reproductive function of adult male offspring born to mothers of different reproductive ages, which is manifested in a decrease in testosterone levels and deterioration of the spermogram, which in turn can lead to problems with impregnation

Long-term function of isotransplanted islets of Langerhans in the diabetic rat

1976 ◽  
Vol 54 (6) ◽  
pp. 916-925 ◽  
Author(s):  
E. J. Greenglass ◽  
J. M. Martin
Keyword(s):  
Insulin Secretion ◽  
Diabetic Rats ◽  
Male Rats ◽  
Diabetic Rat ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Experimental Animals ◽  
Functional Development ◽  
Experimental Group

Highly inbred male rats were made diabetic by subtotal pancreatectomy. After diabetes developed, the animals were transplanted with an average of 500 isolated islets, obtained from donors of the same sex and strain, into the portal vein. Groups of islet-transplanted diabetic rats were followed for periods of 3, 6, and 14 months, during which time the performance of the transplanted islets was investigated. Control animals were followed for similar periods of time. The findings obtained from each experimental group were integrated so as to provide a continuous picture of the transplanted islet's behaviour from 2 weeks to 14 months post transplantation.To assess islet performance, normal, untreated diabetic, and transplanted animals were subjected to three stimulatory tests of insulin secretion: (1) oral glucose tolerance, (2) intravenous sulfonylurea, and (3) intraperitoneal L-arginine. Normal and transplanted rats were additionally subjected to one inhibitory test of insulin secretion, diazoxide (given orally). At the end of the study, control and experimental animals were killed, and morphological investigations were performed on liver and pancreas.Isotransplantation of islets in partially depancreatized diabetic rats resulted in the reversal of the condition. The transplanted islets were found to respond to physiologic and pharmacologic controls, although the characteristics of the responses were not identical with that of the normal intact islet. The results of this study have suggested that a functional development of the transplanted islets occurs.

Testicular Changes in Newborn Rats Exposed to Phototherapy

1999 ◽  
Vol 2 (4) ◽  
pp. 333-336 ◽  
Author(s):  
H. Koç ◽  
H. Altunhan ◽  
A. Dilsiz ◽  
A. Kaymakçi ◽  
S. Duman ◽  
...  
Keyword(s):  
Reproductive System ◽  
Degenerative Changes ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Study Group ◽  
Histopathological Changes ◽  
Newborn Rats ◽  
Long Term Effects ◽  
Fertilization Rates

In this study we investigated the long-term effects of 72-h continuous phototherapy on the reproductive system of newborn rats. The animals' weight, fertilization rates, and number of newborn and histopathological changes in the gonads in a normal group not exposed to phototherapy and in the test animals were compared. At the age of 24 weeks there were no significant differences between the two groups, apart from the histology of the testicles of the male rats who were exposed to the phototherapy. The study group showed a significantly reduced diameter of the seminiferous tubules when compared to the controls ( P < 0.001). It can be postulated that phototherapy may cause histological degenerative changes in the structure of the rat's testes, even though there were no changes in fertilization rates. Further studies are necessary to reveal the effects of phototherapy on humans and to determine the effects, if any, on fertility.

scholarly journals Assessment of sperm production and reproductive organs of Wistar rats to long-term exposure of Caesalpinia ferrea

2010 ◽  
Vol 82 (4) ◽  
pp. 907-914 ◽  
Author(s):  
Leda M.F. Lucinda ◽  
Camila B. Rocha ◽  
Maycon M. Reboredo ◽  
Vinícius C. Faria ◽  
Rita C.S. Sá
Keyword(s):  
Reproductive System ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Reproductive Organs ◽  
The Body ◽  
Male Rats ◽  
Sperm Production ◽  
Male Wistar Rats ◽  
The Right

Caesalpinia ferrea Mart (Leguminosae) is a medicinal plant used to treat diabetes, among other therapeutic properties, but which is also reported to have hepatotoxic effects. Although it contains substances such as flavonoids and coumarin, which are known to have antifertility activity, no studies have apparently been conducted to evaluate the potential adverse side effects of this plant on the function of the reproductive system after a chronic treatment. Therefore, this investigation was carried out to evaluate the effect and safety of the long-term exposure to C. ferrea on male Wistar rats' vital organs, reproductive system and sperm production. Adult and immature male rats were treated with an aqueous extract of C. ferrea at a dose level of 300 mg/kg of body weight, administered during one or two spermatogenic cycles of this species. The reproductive and vital organs were analyzed, and sperm was collected from the epididymal secretion of the right epididymis cauda. The long-term administration of C. ferrea did not significantly alter the body, vital and reproductive organs weights. Gamete production was not affected either. The chronic assessment of C. ferrea suggests that this plant does not affect the normal functioning of the Wistar rat reproductive system.

scholarly journals Options of modeling diet-induced and combined metabolic syndrome

2020 ◽  
Vol 19 (6) ◽  
pp. 346-354
Author(s):  
Anna V. Abramtsova ◽  
Natalia V. Efimenko ◽  
Valentina F. Reps ◽  
Agnessa S. Kaisinova
Keyword(s):  
Metabolic Syndrome ◽  
White Male ◽  
Male Rats ◽  
Control Group ◽  
Physical Factors ◽  
Metabolism Regulation ◽  
The Metabolic Syndrome ◽  
Rank Correlation Method ◽  
Experimental Group

Background. Due to the fact that it is not always possible to reproduce all known manifestations of carbohydrate and lipid metabolism disorders, for example, in rodents, it is necessary to verify individual pathogenetic links in animals when modeling the metabolic syndrome in order to select the most optimal natural biotropic factors for studying the effect on them. Aims: To analyze metabolic disorders in the modeling of metabolic syndrome of alimentary genesis in comparison with the combined effect of alimentary factor and chemical agents to select the most appropriate model in the study of natural and weak preformed physical factors. Materials and methods. Metabolic syndrome was modeled in 47 sexually mature outbred white male rats using a hypercaloric diet (HD) of various duration and medications. The first series was the 1st control group (CG1) and the 1st experimental group (EG1). They received HD during 180 days, they were withdrawn from the experiment 30 days after the cancellation of HD. The second series was the 2nd control group (CG2) and three experimental groups. They received HD for 60 days, where the 2nd group (EG2) in association with HD received Mercazolil (10 mg/kg of animal weight) intragastrically for 14 days starting from the 21st day; the 3rd group (EG3) had the same scheme of treatment as in EG2, besides, after taking Mercazolil, they were intramuscularly injected with Nitox 200 at 25 mg/kg once per day during 5 days. Animals of EG2 and EG3 were withdrawn from the experiment 60 days after ending of HD. Animals of the 4th experimental group (EG4) got HD and the same medicine as in EG3. They left the experiment 30 days after ending HD. The content of hormones and biochemical parameters of protein, fat, and carbohydrate in peripheral blood was evaluated using multiple inter-group comparisons and intra-group relationships by Spearmans rank correlation method. Results. There have been detected a large number of biochemical signs of dependence of metabolism regulation on leptin level after long-term HD (180 days), while there were fewer biochemical signs of protein exchange disorder less than with the combined use of HD (60 days) and drugs. Conclusion. It is more appropriate and promising to use a long-term HD as a model of MS close to the natural conditions of its formation, to study the influence of natural and weak preformed physical factors.

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