Isolation and molecular detection of avian mycoplasmosis in selected areas of Mymensingh district in Bangladesh

2021 ◽  
Vol 7 (2) ◽  
pp. 182-190
Author(s):  
Parinita Basak ◽  
Banya Banowary ◽  
Safeth Arju ◽  
Mohammad Zakir Hossain
Keyword(s):  
Mononuclear Cells ◽  
Research Work ◽  
Mycoplasma Gallisepticum ◽  
Effective Control ◽  
Histopathological Study ◽  
Dna Ladder ◽  
Field Samples ◽  
Avian Mycoplasmosis ◽  
Histopathological Studies ◽  
Hematoxylin And Eosin Stain

Avian mycoplasmosis caused by several species of Mycoplasma including Mycoplasma gallisepticum, M synoviae, M. meleagridis and M. iowae. Among these Mycoplasma gallisepticum is the most important poultry pathogen in Bangladesh. For effective control of Mycoplasmosis, proper early diagnosis is the corner stone. The research work was designed, a total of 20 samples, lung exudates, swabs from trachea and air sacs were collected from dead birds of different poultry farms in Mymensingh district during October-December, 2007. Samples were collected in 10% buffered formalin for histopathological study. Swabs were collected in mycoplasma broth supplemented with supplement-G. Additionally Kanamycin solution was added to prevent the growth of gram–Ve bacteria and then the organisms were transferred into mycoplasma agar for isolation. Histopathological studies were conducted using routine procedure in Hematoxylin and Eosin stain. Isolated Mycoplasma were subjected to DNA extraction, Nested PCR was done using a commercial PCR kit. The histopathological study revealed the presence of mycoplasmal related tissue changes, such as severe congestion and infiltration of mononuclear cells in different organs. The extracted DNA accumulated at the upper position of DNA ladder as band without any smear formation. The DNA from avian mycoplasmas was amplified and gave amplified product of 975 bp by outer primer and 395 bp by inner primer which was much smaller than the expected size. In this study, preliminary results from field samples suggest that culture using mycoplasma agar and broth supplement with Supplement-G and Kanamycin solution could be useful for the isolation of pathogenic avian mycoplasmas. Asian J. Med. Biol. Res. 2021, 7 (2), 182-190

scholarly journals Effect of β-Cyclodextrin Stabilized Silver Nanoparticles on Gills, Kidney, Liver of Danio rerio

2021 ◽  
Vol 11 (1) ◽  
pp. 2981-2995
Keyword(s):  
Silver Nanoparticles ◽  
Danio Rerio ◽  
Morphological Changes ◽  
Antimicrobial Property ◽  
Research Work ◽  
Model Organism ◽  
Histopathological Study ◽  
Histopathological Studies ◽  
Kidney Liver ◽  
Drug Studies

The silver nanoparticles (AgNP) are applied broadly in medical applications due to their antimicrobial property. However, the toxicity (uptake, translocation, and accumulation) of these AgNPs nanomaterial has not been much explored. Also, cyclodextrin has been used for different pharmaceutical applications due to its various potential properties. Therefore, the toxicity of these AgNPs and cyclodextrin in the model organism such as Danio rerio could be crucial for future nano-drug studies. The main aim of the present research work is to synthesize, characterize biopolymeric silver nanoparticles, and perform histopathological studies of synthesized silver nanoparticles on Danio rerio. The silver nanoparticles of 130nm size have been synthesized successfully using β-Cyclodextrin (β-CD) at room temperature. The various analytical applied to characterized the β-Cyclodextrin (β-CD) reduced silver nanoparticles (β-CD)-SNPs. A histopathological study has been conducted to evaluate the toxicity effect of the synthesized (β-CD)-SNP on the animal model Danio rerio. The (β-CD)-SNPs conc. 30µl/l is affecting and damaged gills and kidney the Danio rerio organs (gills, kidney) exposed after 10 days, yet the liver was found to be healthy. In conclusion, the Danio rerio gills, kidneys, and liver are sensitive to the 130nm-sized (β-CD)-SNPs. The nanoparticles' toxicity depends on concentration; less concentration (30µl/l) accumulates and is absorbed efficiently than the higher concentration (300µl/l). However, no morphological changes were observed on Danio rerio.

scholarly journals Seroprevalence and pathological study of Salmonella infections in layer chickens and isolation and identification of causal agents

1970 ◽  
Vol 4 (2) ◽  
pp. 79-85 ◽  
Author(s):  
MM Islam ◽  
MG Haider ◽  
EH Chowdhury ◽  
M Kamruzzaman ◽  
MM Hossain
Keyword(s):  
Mononuclear Cells ◽  
Research Work ◽  
Liver Parenchyma ◽  
Muscularis Mucosa ◽  
Pathological Study ◽  
Isolation And Identification ◽  
Bacterial Colony ◽  
Focal Necrosis ◽  
Salmonella Infections ◽  
Layer Chickens

The present research work was conducted to study about the seroprevalence and pathology of Salmonella infections in layer chickens of Dhaka and Gazipur regions of Bangladesh and to isolate and characterize Salmonellae from layer chickens during the period from January to May 2006. The used materials were blood sample, cloacal and liver swabs from live and dead birds respectively, and visceral organs (liver, lungs, spleen and intestine). The used methods were serum plate agglutination (SPA) test; necropsy and histopathology; cultural, morphological and biochemical test. The overall seroprevalence was 43.4%. During necropsy, congested and enlarged liver with focal necrosis, haemorrhagic and discoloured ovary with stalk formation and mild haemorrhagic to catarrhal enteritis in intestine were detected. Microscopically, the liver showed congestion, focal necrosis with multifocal infiltration of histiocytes in liver parenchyma. In ovum, infiltration of leukocytes, bacterial colony, RE cell proliferation and fibrinoid necrosis were recorded. The intestinal mucosa exhibited infiltration of mononuclear cells in mucosa, submucosa as well as muscularis mucosa. A total of 33 (21.02%) Salmonellae from live and dead birds were isolated. The isolation rate of Salmonellae was higher in seronegative (31.6%) group than seropositive (3.2%) group. Out of 33 Salmonella isolates, 25 were S. pullorum, 3 were S. gallinarum and the rest 5 were motile Salmonellae. The isolates obtained in the study may be investigated for serotyping, pathogenesis study, antibiogram and vaccine production in future. Key words: Seroprevalence, histopathology, isolation, identification, Salmonella, chickensDOI = 10.3329/bjvm.v4i2.1288Bangl. J. Vet. Med. (2006). 4 (2): 79–85

scholarly journals HEPATOPROTECTIVE AND NEPHROPROTECTIVE EFFECTS OF THE AQUEOUS EXTRACT OF TURMERIC (CURCUMA LONGA) IN RIFAMPICIN AND ISONIAZID-INDUCED HEPATOTOXICITY AND NEPHROTOXICITY IN RATS

2019 ◽  
pp. 293-298
Author(s):  
MAHDI M THUAWAINI ◽  
MAWAHIB B GASIM AL-FARHAAN ◽  
KARIMA F ABBAS
Keyword(s):  
Aqueous Extract ◽  
Total Bilirubin ◽  
Curcuma Longa ◽  
Albino Rats ◽  
Histopathological Study ◽  
Protective Effects ◽  
Significant Elevation ◽  
Liver And Kidney ◽  
Histopathological Studies ◽  
Kidney Functions

Objectives: The present study was designed to estimate the influences of oral administration of aqueous extract of turmeric (Curcuma longa) in hepatotoxicity and nephrotoxicity induced in rats by isoniazid and rifampicin (RIF) for 4 weeks. Influences were determined through the estimation of liver and kidney functions and histopathological changes. Materials and Methods: A total of 48 male albino rats were randomly divided into six groups: Normal control, INH+RIF treated rats, Turmeric aqueous extract 100 mg/kg treated rats, Turmeric aqueous extract 100 mg/kg + INH and RIF treated rats, Turmeric aqueous extract 200 mg/kg treated rats, Turmeric aqueous extract 200 mg/kg+ INH and RIF treated rats. Turmeric aqueous extract and INH + RIF (50 mg/kg bwpo, daily) were given for 4 weeks. Liver and kidney function markers (aspartate transaminase [AST], alanine transaminase [ALT], alanine phosphatase [ALP], bilirubin, blood urea, and creatinine) were determined enzymatically. In addition, tissues of liver and kidney were quickly separated and fixed in 10% formalin and subjected to histopathological studies. Statistical analysis was carried out using t-test. Results: The aqueous extract of turmeric (at a dose of 100 and 200 mg/kg bw, p.o. daily ) showed hepato- and reno-protective effects in hepato- and reno- toxicity induced by RIF and INH in rats. Significant elevation of serum ALT, AST, ALP, total bilirubin, creatinine, urea, and total protein, due to RIF and INH treatment, were significantly decreased. The histopathological study further confirmed the biochemical results. Conclusion: Results of the present study indicated that turmeric has hepatoprotective and renoprotective action against RIF- and INH-induced hepatic and renal injury in rats.

scholarly journals Histopathological study of endometrium in abnormal uterine bleeding

2012 ◽  
Vol 2 (4) ◽  
pp. 297-300 ◽  
Author(s):  
S Bhatta ◽  
AK Sinha
Keyword(s):  
Histopathological Examination ◽  
Abnormal Uterine Bleeding ◽  
Uterine Bleeding ◽  
Common Finding ◽  
Histopathological Study ◽  
Endometrial Sampling ◽  
Proliferative Endometrium ◽  
Malignant Lesions ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Background: Histopathological evaluation of endometrial samples is essential in the workup of abnormal uterine bleeding. This study was carried out to evaluate patterns of endometrial histological findings in women with abnormal uterine bleeding and to correlate histopathological findings with clinical features.Materials and Methods: One hundred and twenty two patients with diagnosis of abnormal uterine bleeding underwent endometrial sampling. The slides stained with Hematoxylin and Eosin stain were studied.Results: The predominant endometrial histopathological finding was proliferative endometrium 32 cases (26.23%) followed by simple hyperplasia without atypia 22 cases (18.03%). Malignant lesions were more common in patients more than 40 years of age and comprised of 7 cases (5.74%) of all cases. Atrophic endometrium was most common finding in postmenopausal bleeding 8 cases (28.6%) followed by endometrial carcinoma 5 cases (17.9%).Conclusion: Histopathological examination of endometrium should be done generously in women presenting with abnormal uterine bleeding especially after the age of 40 years to rule out malignant pathology.Journal of Pathology of Nepal (2012) Vol. 2, 297-300DOI: http://dx.doi.org/10.3126/jpn.v2i4.6882

Control of Cell Death Levels Using Th9402-Based PDT Treatment on Fresh PBMC, and Potential Application to Patients with cGvHD.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5258-5258
Author(s):  
Annie Levesque ◽  
Ann-Louise Savard ◽  
Denis-Claude Roy ◽  
Francine Foss ◽  
Christian Scotto
Keyword(s):  
Cell Death ◽  
Cell Apoptosis ◽  
Immune Modulation ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Apoptotic Cell ◽  
Tunel Assay ◽  
Clinical Activity ◽  
Proliferating Cells ◽  
Dna Ladder

Abstract Recently, extracorporeal photopheresis (ECP) has shown interesting clinical activity for the treatment of drug-refractory chronic graft-vs-host disease (cGvHD), inducing Th1/Th2 immunomodulation to restore immune tolerance. Several studies indicate that target cell apoptosis may have a role in the control of cGVHD, and increasing apoptotic levels may favor immune modulation. We have developed an approach to eliminate immunoreactive cells using the Theralux™ photodynamic cell therapy (PDT) system based on the use of the rhodamine-derivative TH9402 illuminated ex vivo with a visible light source (l=514nm). The capacity of TH9402 PDT to induce increasing levels of T cell apoptosis has not been investigated. The induction of apoptotic cell death was studied using peripheral blood mononuclear cells (PBMCs) isolated from healthy volunteers (HV) and cGvHD patients. We found a good correlation between PDT conditions and levels of cell death induced. In comparison to controls showing 8±4 percent (%) of apoptosis, as measured by TUNEL assay on cells harvested 3 days post-treatment, PBMC from HV subjected to PDT using 0.33, 0.66, or 1.32 micromolar of TH9402 showed 28±16%, 49±17%, and 78±11% of apoptosis, respectively. These studies have also shown that the intra- and inter-donor variability in TH9402 incorporation are very low (~5% and 10%, respectively). To ensure that these findings could also be applied to a clinical setting, PBMC from cGvHD patients were treated with 0.33 and 1.32 micromolar of TH9402 to trigger either low or high levels of apoptosis. PBMC from cGvHD patients showed a sensitivity similar to that of PBMC from HV, with 38±9% and 73±13% of apoptosis when treated with 0.33 and 1.32 micromolar TH9402, respectively. Data obtained from the analysis of various cell death parameters such as the loss of DΨm, AnV/PI staining, TUNEL assay and agarose DNA ladder on PBMC and human Jurkat T cells treated with escalating doses of TH9402 suggested strongly the higher sensitivity of proliferating cells to the treatment. Moreover, together with the use of the caspase-inhibitor ZVAD-fmk, these data suggested that upon irradiation, the photoactivation of TH9402 will trigger the formation of reactive oxygen species (ROS) and the release of proapoptotic factors from mitochondria triggering various cell death mechanisms, such as caspase-dependent apoptosis, caspase-independent apoptosis, or a mixture of apoptosis and necrosis. Finally, preliminary data showed that PDT-treated cells were able to induce in vitro the maturation of monocyte-derived dendritic cells. Based on these data, we are beginning a pilot clinical study evaluating two controlled PDT conditions inducing low and high levels of apoptosis in order to assess the efficacy and biological effect of TH9402-based ECP to treat cGVHD in humans.

scholarly journals Identification of Strain-Specific Sequences That Distinguish a Mycoplasma gallisepticum Vaccine Strain from Field Isolates

2016 ◽  
Vol 55 (1) ◽  
pp. 244-252 ◽  
Author(s):  
Camir Ricketts ◽  
Larissa Pickler ◽  
John Maurer ◽  
Saravanaraj Ayyampalayam ◽  
Maricarmen García ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Reference Genome ◽  
Hypothetical Protein ◽  
Mycoplasma Gallisepticum ◽  
Economic Losses ◽  
Poultry Production ◽  
Content Type ◽  
Field Isolates ◽  
Avian Mycoplasmosis ◽  
A Cell

ABSTRACTDespite attempts to control avian mycoplasmosis through management, vaccination, and surveillance,Mycoplasma gallisepticumcontinues to cause significant morbidity, mortality, and economic losses in poultry production. Live attenuated vaccines are commonly used in the poultry industry to control avian mycoplasmosis; unfortunately, some vaccines may revert to virulence and vaccine strains are generally difficult to distinguish from natural field isolates. In order to identify genome differences among vaccine revertants, vaccine strains, and field isolates, whole-genome sequencing of theM. gallisepticumvaccine strain ts-11 and several “ts-11-like” strains isolated from commercial flocks was performed using Illumina and 454 pyrosequencing and the sequenced genomes compared to theM. gallisepticumRlowreference genome. The collective contigs for each strain were annotated using the fully annotatedMycoplasmareference genome. The analysis revealed genetic differences amongvlhAalleles, as well as among genes annotated as coding for a cell wall surface anchor protein (mg0377) and a hypothetical protein gene,mg0359, unique toM. gallisepticumts-11 vaccine strain. PCR protocols were designed to target 5 sequences unique to theM. gallisepticumts-11 strain:vlhA3.04a,vlhA3.04b,vlhA3.05,mg0377, andmg0359. All ts-11 isolates were positive for the five gene alleles tested by PCR; however, 5 to 36% of field isolates were also positive for at least one of the alleles tested. A combination of PCR tests forvlhA3.04a,vlhA3.05, andmg0359was able to distinguish theM. gallisepticumts-11 vaccine strain from field isolates. This method will further supplement current approaches to quickly distinguishM. gallisepticumvaccine strains from field isolates.

scholarly journals Reduction in cisplatin genotoxicity (micronucleus formation) in non target cells of mice by protransfersome gel formulation used for management of cutaneous squamous cell carcinoma

2011 ◽  
Vol 61 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Vandana Gupta ◽  
Ramesh Agrawal ◽  
Piyush Trivedi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Skin Permeation ◽  
Research Work ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Histopathological Study ◽  
Target Cells ◽  
Micronucleus Formation

Reduction in cisplatin genotoxicity (micronucleus formation) in non target cells of mice by protransfersome gel formulation used for management of cutaneous squamous cell carcinomaCisplatin-loaded protransfersome system was prepared and characterized forin vitrodrug permeation, drug deposition and antitumor effect. A histopathological study and a genotoxicity study were also done. The skin permeation data of cisplatin from protransfersome gel formulation revealed 494.33 ± 11.87 μg cm-2, which was significantly higher than that from the control plain drug solution in 0.9 % NaCl (p< 0.001). Untreated group of animals showed invasive moderately differentiated keratinizing squamous cell carcinoma (malignant stage). However, with cisplatin loaded protransfersome gel system simple epithelial hyperplasia (pre-cancerous stage) with no cancerous growth was observed. Also, a significant induction in micronucleus formation was found in the group that was treated with injectable intraperitoneal cisplatin preparation in 0.9 % saline as compared to the group treated with topical protransfersome gel formulation. The findings of this research work appear to support improved, site-specific and localized drug action in the skin, thus providing a better option for dealing with skin related problems like squamous cell carcinoma.

scholarly journals Investigation on Seroprevalence of Mycoplasma Gallisepticum Infection in Commercial Layer Farms in Rajshahi District Using Rapid Serum Plate Agglutination Test

2021 ◽  
Vol 3 (4) ◽  
pp. 31-36
Author(s):  
Aurangazeb Kabir ◽  
Md. Obaidullah Al Masum ◽  
Most. Sabina Yasmin ◽  
Md. Golam Sarwar ◽  
Md. Shamim Hossain ◽  
...  
Keyword(s):  
Respiratory Infections ◽  
Winter Season ◽  
Mycoplasma Gallisepticum ◽  
Agglutination Test ◽  
Preventive Strategies ◽  
Security Measures ◽  
Prophylactic Measures ◽  
Avian Mycoplasmosis ◽  
Financial Losses ◽  
Sera Samples

Mycoplasma gallisepticum (MG) infection is very common in birds that cause respiratory infections in chickens, turkeys, and other avian species. It has brought about a considerable amount of financial losses to the poultry sector in Bangladesh. We conducted a study on the Seroprevalence of MG infection in two different geographical areas of Bangladesh under Rajshahi district, namely Paba and Bagmaraupazila. 800 sera samples were collected and tested with Rapid Serum Plate Agglutination Test (RSA) to identify the MG antibodies using commercial Mycoplasma gallisepticum antigen. The gross Seroprevalence of MG infection was 59.25% in the study area. The maximum rate (68.80%) of infection was found in the winter season, followed by the summer season (49.36%). The result further revealed that the condition was higher (69.01%) in larger-sized flocks than in small (53.63%). We noticed that younger birds having 10-20 weeks of age are more prone to be affected with avian mycoplamosis with an infection rate of 72% compared to their adult counterpart of 71+ weeks with 52% morbidity. Our study revealed that Mycoplasma gallisepticum infection is prevalent in Paba and Bagmaraupazila in Rajshahi. The farms should take strict bio-security measures to mitigate this infection in the mentioned areas. Proper medications for the affected birds and timely prophylactic measures for the healthy ones could be practical and preventive strategies against avian mycoplasmosis. Amid limitations, we conducted our experiments, and thus further research is warranted to substantially assess and validates our observations.

scholarly journals Histopathological Study of Endometritis of the cows

2010 ◽  
Vol 9 (1) ◽  
pp. 69 ◽  
Author(s):  
A. Gh. Rhyaf
Keyword(s):  
Light Microscope ◽  
Inflammatory Cells ◽  
Histopathological Study ◽  
Epithelial Layer ◽  
Histopathological Changes ◽  
Uterine Tissue ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Uterine Glands

The present study was designed to investigate histopathological changes of endometritis in cows. 32 specimens of uteri of normal cows were collected from AL-Diwanyia slaughterhouse during 3 months twice weekly. All these specimens were cut and prepared for histopathological sections, then stained with hematoxylin and eosin stain and it examined under a light microscope, the result showed hyperplasia of the epithelial layer of the uterus, severe infiltration of inflammatory cells, atrophy of uterine glands with hemorrhage and serious edema in the uterine tissue in some cases.

scholarly journals Pathological Effects of Crud Trichothecenes on Swiss Mice

2010 ◽  
Vol 4 (1) ◽  
pp. 20-27
Author(s):  
Noor Hashim ◽  
Khulood Al-Samarrae ◽  
Salim Al-Obaidy
Keyword(s):  
Mononuclear Cells ◽  
Health Hazard ◽  
Farm Animals ◽  
Economic Losses ◽  
Histopathological Study ◽  
Treated Animal ◽  
Histopathological Changes ◽  
Retention Cyst ◽  
Portal Area ◽  
Biochemical Studies

Trichothecenes are natural secondary metabolites causing economic losses and health hazard to human and farm animals which are produced by several species of Fusarium and some other genera on different agricultural commodities. Study on trichothecenes mycotoxicosis revealed morphological, biochemical, and histopathological changes. After intraperitoneal injection of the toxin in male mice with different concentration for 35 days shows marked increase in body weight, dyspanea, shivering, bristling up of hair, hair falling, anomalies of eyes, and irritation around neck, also abdominal hemorrhage and clot accumulation in abdomen. In addition to inclusion (retention) cyst forms on liver. The biochemical studies on liver function by measuring GPT and GOT enzymes level have been done. An increase level of these enzymes in treated animal in comparison with control animal which indicating abnormal function of liver observed. The histopathological study on sections from the liver of treated animal with trichothecenes revealed many alterations in liver which includes congestion, kupffer cells hyperplasia, dilated sinusoids and mononuclear cells infiltration around the portal area.

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