scholarly journals Isolation of Aerobic Bacteria from Surgical Site Infection and their Antibiotic Susceptibility Pattern

2017 ◽  
Vol 2 (2) ◽  
pp. 28-32
Author(s):  
Shahin Ara Begum ◽  
Shabeen Afreen ◽  
Aliya Rashid ◽  
Nasreen Farhana
Keyword(s):  
Staphylococcus Aureus ◽  
Surgical Site Infection ◽  
Antibiotic Susceptibility ◽  
Aerobic Bacteria ◽  
Site Infection ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
Gram Negative ◽  
Causative Agents ◽  
Gram Positive Cocci

Background: Surgical site infection is one of the commonest complications after surgical intervention. Source of wound infections may be exogenous or endogenous. Wound swabs culture is the most frequently used method for confirming the diagnosis of surgical site infection. A regular bacteriological review is necessary to identify the causative agents and their antibiotic susceptibility pattern.Objective: The purpose of the present study was to find out the aerobic bacteria from wound swabs culture and their antibiotic susceptibility pattern.Methodology: A total of 175 wound swabs sample were collected aseptically from surgical site infection during the study period. Standard bacteriological methods were used for isolation and identification of organisms and their susceptibility pattern.Results: A total of 175 wound swab samples were collected from surgery departments. Out of 175 samples 102 (58.28%) were positive by culture. Among the isolated organisms the gram negative bacilli were predominant 67 (65.68%) than the gram positive cocci 35 (34.32%).The most common isolated bacteria were Escherichia.coli 30(29.41%) followed by Staphylococcus aureus 28 (27.45%) Pseudomonous areuginosa 20(29.85%), Acinetobacter spp 08(11.94%), Coaggulase negative Staphylococcus (CONS) 07(6.86%) Klebsiella pneumoniae 05(4.90%), Proteus spp 03(4.45%), and Citobacter spp 01(1.49%). Among the total 175 samples 114(65.14%) were collected from male patients and 61(34.86%) were from female patients. All the isolated gram negative bacilli showed resistant to all antibiotics and all isolated gram positive cocci were resistant to all antibiotics except Linezolid. Among the isolated Staphylococcus aureus 8 (24.85%) were MRSA and one (3.57%) were VRSA. The most effective antibiotics were Amikacin, Meropenem, Imipenem and Tazobactam in this study.Conclusion: Surgical site infections remain the commonest post operative complications after surgery and one of the commonest encountered hospital acquired infections. Therefore, periodic review needs be done to find out the causative agents and their antibiotic susceptibility pattern for better treatment and managementBangladesh Journal of Infectious Disease 2015;2(2):28-32

scholarly journals Microbiological Profile of Uropathogens and their Antibiotic Susceptibility Pattern in Pediatric Population from A Tertiary Care Hospital in Amritsar, Punjab

2021 ◽  
Vol 10 (11) ◽  
pp. 306-312
Author(s):  
Manpreet Kaur Bhatha Loveena Oberoi ◽  
Sapna Soneja Anuradha Malhotra ◽  
Kamaldeep Singh
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Pediatric Population ◽  
Age Group ◽  
Susceptibility Pattern ◽  
Gram Positive ◽  
Antibiotic Susceptibility Pattern ◽  
Gram Negative ◽  
Gram Negative Organisms ◽  
Gram Positive Cocci

Urinary tract infection(UTI) is one of the most commonest bacterial infection encountered in the pediatric age group. Early diagnosis is of utmost importance to preserve renal function and reduce long term complications such as renal scarring. The objective of this study was to determine the prevalence of UTI in pediatric population, identify the common uropathogens and study the antibiotic susceptibility pattern of bacterial isolates. Materials and Methods: A retrospective study was carried out in Department of Microbiology, GMC Amritsar for a period of 6 months from 1 Jan 2021 till 30 June 2021 amongst the pediatric patients (1 month-12 years age group) with clinically suspected UTI including both indoor and outdoor patients. Clean catch midstream urine samples received in the laboratory were processed as per the standard microbiological guidelines. Antibiotic susceptibility testing was done using Kirby-Bauer disc diffusion method as per CLSI guidelines. Results: A total of 549 samples were received and processed during study period from which 164(29.87%) were culture positive. Culture positivity was more prevalent in females (61.58%) than males (38.41%). Gram negative organisms were predominant (74.4%) followed by gram positive cocci (22.56%) and Candida (3.05%). Among gram negative organisms Escherichia coli was most predominant followed by Klebsiella spp., Pseudomonas spp., Acinetobacter spp., Proteus spp. and Citrobacter spp. Among gram positive cocci Staphylococcus aureus was most predominant followed by Coagulase negative Staphylococci and Enterococcus spp. Majority of gram negative bacilli were resistant to fluroquinolones, 3rd generation cephalosporins and co-trimoxazole while nitrofurantoin, piperacillin-tazobactam and gentamicin were most sensitive drugs. Among Staphylococcus aureus, methicillin resistance was seen in 17% isolates. High sensitivity was seen to linezolid and vancomycin in gram positive cocci. Conclusion: This study highlights increased prevalence of MDR uropathogens in pediatric population which indicates that antibiotic selection should be based on knowledge of local prevalence of bacterial organisms and their antibiotic sensitivities with rational use of antibiotics.

Prevalence of surgical site infection and antibiotic susceptibility pattern among post-operative patients at a tertiary hospital in Lafia Nasarawa state, Nigeria

2021 ◽  
Vol 7 (4) ◽  
pp. 117-120
Author(s):  
Ikrama Hassan ◽  
◽  
Michael Anazodo ◽  
Agbo Christian Agbo ◽  
Oga Esa Ochapa ◽  
...  
Keyword(s):  
Surgical Site Infection ◽  
Antibiotic Susceptibility ◽  
Surgical Patients ◽  
P Value ◽  
Site Infection ◽  
Susceptibility Pattern ◽  
Klebsiella Species ◽  
Mannitol Salt Agar ◽  
Antibiotic Susceptibility Pattern ◽  
Sensitivity Pattern

Background: Surgical site infection is a common challenge to both the surgical patients and the surgical specialists and may lead to morbidity and mortality. The aetiologic agents vary from one place to the other, ditto the antibiotic sensitivity pattern. This study determined the prevalence of surgical site infection, the aetiologic agents and the antibiotic susceptibility pattern in a tertiary health facility in Lafia. Methods: The study was carried out over 5 (2015 – 2019) year period on post operative patients at the Dalhatu Araf Specialist Hospital (DASH) in the surgical and obstetrics and gynaecological wards. Swab taken from infected wounds were cultured using 2 plates of blood agar, MacConkey agar and Mannitol salt agar. The antibiotic susceptibility testing was done using Kirby Bauer technique. The data was analysed using SPSS version 23. Results obtained were presented in tables. Significant p value is < 0.05. Results: The mean age of the respondents is 28.6 ± 11.2 years. Of the 408 surgical patients seen within the 5 year period, 15 (3.7%) had surgical sites infection given a prevalence of 3.7%. A total of 6.6% of the patients who had undergone surgeries were immune-compromised. Escherichia coli and Klebsiella species were the two cultured organisms accounting for 75% and 25% respectively. Antibiotic susceptibility revealed that all (100%) were sensitive to the quinolone (ciprofloxacin). Conclusion: Surgical site infection is most common in the third decade of life with a mean age of 28.6 ± 11.2 years. There is a low prevalence of surgical site infection from this study. Eschericia coli and Klebsiella species were the two common organism isolated with both having a good sensitivity to ciprofloxacin

scholarly journals Bacteriological Profile, Antibiotic Susceptibility Pattern and Predictors of bacteremia among Children with Bloodstream Infection

2021 ◽  
Author(s):  
Samina Nazir Chaki ◽  
Levina Msuya ◽  
Deborah Mchaile ◽  
Michael Johnson Mahande ◽  
Ronald Mwitalemi Mbwasi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bloodstream Infection ◽  
Antibiotic Susceptibility ◽  
Klebsiella Pneumonia ◽  
Gram Negative Bacteria ◽  
Susceptibility Pattern ◽  
Gram Positive ◽  
Antibiotic Susceptibility Pattern ◽  
Gram Negative

Abstract Background: Bloodstream infections are major causes of morbidity and mortality among children in Sub-Saharan countries. This study aimed to determine the prevalence of bacteremia, etiological agents’ antibiotic susceptibility pattern and predictors of bacteremia among children with bloodstream Infection.Methodology: This hospital-based cross-section study involved children aged two months to thirteen years. All children meeting the inclusion criteria during the study period were enrolled. All consenting parents were interviewed via a questionnaire to collect data, followed by a thorough physical assessment and venipuncture was done to collect blood samples. Data were analysed using SPSS version 23.Results: Among 242 study participants, 154(63.6%) were male and blood cultures were positive in 37(15.3%). Gram-positive and gram-negative bacteria constituted 32(80%) and 8(20%), respectively. The frequent pathogen found was Staphylococcus aureus 25(62.5%), followed by Enterococcus spp. 4(10%), Escherichia coli 4(10%), Pseudomonas aeruginosa 3(7.5%), Streptococcus pyogenes 3(7.5%) and 1(2.5%) Klebsiella pneumonia. The majority of bacterial isolates showed high resistance to commonly used antibiotics in the study area. Predictors of bacteremia were severe malnutrition, hydrocephalus, hyperglycemia, lethargy and BSI with no foci of infection. Conclusion: Prevalence of bacteremia was 15.3%. Gram-positive bacteria were more prevalent than gram-negative bacteria. Staphylococcus aureus and Escherichia coli were the prevalent isolates causing BSI. Effective antibiotics for both gram-negative & gram-positive organisms are imipenem, meropenem and piperacillin-tazobactam followed by amikacin; vancomycin & clindamycin for gram-positive organisms. To curb the growing antimicrobial resistance that we see in this and other studies, continuous antimicrobial stewardship is necessary, else we risk failing to treat BSI.

scholarly journals Changing Trend of Neonatal Septicemia and Antibiotic Susceptibility Pattern of Isolates in Nepal

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Sangita Thapa ◽  
Lokendra Bahadur Sapkota
Keyword(s):  
Staphylococcus Aureus ◽  
Early Onset ◽  
Antibiotic Susceptibility ◽  
Late Onset ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Sensitivity Testing ◽  
Antibiotic Susceptibility Pattern ◽  
Neonatal Septicemia ◽  
Gram Negative

Background. Neonatal septicemia is one of the most common leading reasons for neonatal morbidity and mortality in developing countries. Frequent monitoring on pathogens with recent updates and their antimicrobial sensitivity pattern is mandatory for the better treatment. The aim of the study was to determine the bacteriological profile of neonatal septicemia and their antibiotic susceptibility pattern. Methods. This was a cross-sectional study conducted in Outpatient Department (OPD), Neonatal Intensive Care Unit (NICU), and Pediatrics Ward of Chitwan Medical College Teaching Hospital (CMCTH), Bharatpur, Nepal. Blood cultures were performed on all suspected neonates attending to the hospital with a clinical analysis of neonatal septicemia. Isolated organism was identified by the standard microbiological protocol and antibiotic sensitivity testing was done by Kirby-Bauer disk diffusion method. Results. Out of 516 specimens, bacterial growth was obtained in 56 specimens (10.8%). Prevalence of early onset sepsis was higher 35 (62.5%) in neonates compared to late onset sepsis 21 (37.5%). Majority of neonatal septicemia were caused by gram-negative isolates 39 (69.6%). Acinetobacter species 18 (32.1%) was most commonly isolated organism followed by Staphylococcus aureus 11 (19.6%). The predominant isolate in early onset septicemia was Acinetobacter species 18 (32.1%) and Staphylococcus aureus 9 (16%) and in late onset septicemia was Staphylococcus aureus 11 (19.6%) and Acinetobacter species 5 (8.9%). Staphylococcus aureus and coagulase-negative Staphylococci displayed highest susceptibility towards vancomycin, amikacin, teicoplanin, and meropenem. Gram-negative isolates showed susceptibility towards amikacin, piperacillin/tazobactam, meropenem, ofloxacin, and gentamicin. Conclusions. Acinetobacter species and Staphylococcus aureus remain the most predominant organisms responsible for neonatal septicemia in a tertiary care setting and demonstrate a high resistance to the commonly used antibiotics. Above all, since the rate of Acinetobacter species causing sepsis is distressing, inspiring interest to control the excess burden of Acinetobacter species infection is mandatory.

scholarly journals Profile of Aerobic Bacteria in Surgical Site Infection Following Caesarean Section and Antibiotic Susceptibility Pattern in Silchar Medical College, India

2021 ◽  
Vol 10 (35) ◽  
pp. 3030-3034
Author(s):  
Nazrana Choudhury ◽  
Abul Barkat Fuzayel Ahmed ◽  
Debadatta Dhar Chanda ◽  
Achinta Kumar Borthakur
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Caesarean Section ◽  
Surgical Site Infection ◽  
Site Infection ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Gram Negative ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern

BACKGROUND Caesarean section is one of the most common surgeries performed in women of reproductive age group. Postoperative surgical site infection (SSI) is the third most frequently reported nosocomial infection. Recovery after caesarean section can be more demanding for a woman who develops a post-operative surgical site infection that can even lead to death. Surveillance of surgical site infection is an important infection control activity. A better understanding of the spectrum of pathogens causing SSI as well as their susceptibility pattern is important for prompt management of patients. The main objectives of this study were to identify the bacterial agents causing postoperative wound infection in caesarean section, as well as determine the in-vitro antimicrobial susceptibility pattern of different bacterial isolates and also to examine the risk factors of postoperative caesarean infection. METHODS This is an observational (cross sectional study) where 100 cases of postoperative caesarean section wound infection admitted in the Department of Obstetrics & Gynaecology ̦ Silchar Medical College were studied. Pus samples were collected and cultured, and the pathogenic isolates were tested against different antimicrobial agents by standard disc diffusion method (Kirby-Bauer method) as per the recommendation of Clinical and Laboratory Standard Institute (CLSI) guidelines. RESULTS The study revealed Staphylococcus aureus 38.1 % of which 21.9 % were methicillin resistant (MRSA), as the most common organism responsible for SSI followed by Escherichia coli 23.8 % and Klebsiella pneumoniae 16.7 %. Staphylococcus aureus was 100 % sensitive to vancomycin and linezolid 78.1 % and 71.9 % sensitive to erythromycin and ciprofloxacin respectively. The most sensitive antimicrobials for Gram negative isolates were polymyxin B 96.2 %, imipenem 94.2 % and ciprofloxacin 76.9 %. Emergency C-section (83 %), premature rupture of membranes (PROM) 30 %, prolong labour 26 %, history of previous caesarean section 22 %, anaemia 15 % cases and BMI ≥ 25 (16 %) were found to be risk factors. CONCLUSIONS The findings of preponderance of Staphylococcus aureus and Gram negative bacilli in this study with good antibiotic sensitivity against the fluoroquinolone and aminoglycosides is of local clinical relevance. KEY WORDS Caesarean Section, Surgical Site Infection, Antimicrobial Susceptibility Pattern, Bacterial Isolates, MRSA Strains

scholarly journals Antibiotic susceptibility pattern of staphylococcus aureus and methicillin-resistant staphylococcus aureus in a tertiary care hospital

2014 ◽  
Vol 4 (7) ◽  
pp. 548-551 ◽  
Author(s):  
CP Bhatt ◽  
BMS Karki ◽  
B Baral ◽  
S Gautam ◽  
A Shah ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Penicillin G ◽  
Care Hospital ◽  
Age Group ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
Methicillin Resistant ◽  
Drug Of Choice

Background: Methicillin resistant Staphylococcus aureushas emerged as one of the most important nosocomial pathogens. It invokes a tremendous financial burden and enhanced morbidity and mortality due to difficult to treat systemic infections.Aim of this study was to determine antibiotic susceptibility pattern of Staphylococcus aureus and Methicillin resistant Staphylococcus aureus. Materials and Methods: Different clinical specimens were collected and processed for routine culture and antibiotic sensitivity test by standard microbiology techniques. Results: Out of 1173 samples received for microbiological examination, 100 were found to be S. aureus with 19% cases were Methicillin resistant Staphylococcus aureus (MRSA). Fourteen MRSA were found from inpatient and 5 were from outpatient. MRSA was found higher in female than male and maximum number (31.5%) was found in age group 0-10 years. Staphylococcus aureus was 100% sensitive to Vancomycin followed by Amikacin (90%), Gentamycin (83%), and tetracycline (81%). On urine isolates Nitrofurantoin(91.6%) was drug of choice. All the isolates were resistant to Penicillin G. In case of Methicillin resistant Staphylococcus aureus showed 100% sensitive to Vancomycin followed by Amikacin (84.2%), Tetracycline (63.1%), Ciprofloxacin (42%) and Gentamycin (36.8%). Among urine isolates Nitrofutantoin showed 87.5% sensitive followed by Norfloxacin (75%). Conclusion: Methicillin resistant Staphylococcus aureus was found 19% of Staphylococcus aureus isolates. It was most common in females, hospitalized patients and young age group. Vancomycin seems to be drug of choice followed by Amikacin. It would be helpful to formulating and monitoring the antibiotic policy and ensure proper empiric treatment. DOI: http://dx.doi.org/10.3126/jpn.v4i7.10297 Journal of Pathology of Nepal (2014) Vol. 4, 548-551   

Sign in / Sign up

Export Citation Format

Share Document