scholarly journals Antimicrobial and anti-oxidant potential of Periploca hydaspidis

2015 ◽  
Vol 10 (3) ◽  
pp. 645 ◽  
Author(s):  
Rafi Ullah ◽  
Jehan Bakht ◽  
Mohammad Shafi
Keyword(s):  
Antioxidant Activity ◽  
Growth Inhibition ◽  
Methanolic Extract ◽  
Maximum Growth ◽  
Zone Of Inhibition ◽  
Aqueous Fraction ◽  
E Coli ◽  
Methanolic Extracts ◽  
Anti Oxidant ◽  
Diffusion Assay

<p>The present study investigates antimicrobial and anti-oxidant potential of different solvent extracted samples from <em>Periploca hydaspidis </em>through disc diffusion assay. The data revealed that all the extracts reduced the growth of the tested microbes using three different concentrations. <em>Pseudomonas aeruginosa </em>and <em>E. coli</em> were sensitive to crude extracts and all fractions measuring varying degree of growth inhibition.  Similarly, the growth of <em>Citrobacter freundii</em> was not inhibited by crude methanolic extracts, n-hexane, ethyl acetate and aqueous extracted samples. Maximum growth inhibition was measured against <em>Klebsiella pneumoniae</em> exposed to aqueous fraction followed by n-hexane fraction. Our results also suggested that among various fungi, <em>Candida albicans</em> was sensitive to crude methanolic extracts, n-hexane and aqueous fractions compared with other fractions. The rest of the fungi under test were resistant to crude and all fractions showing no zone of inhibition. All the extracted samples of the stems and roots showed antioxidant activity, however, crude methanolic extract of stem tissues exhibited better antioxidant activity than the other extracts.</p>

scholarly journals Effect of Methanolic Extract of Caryota no on Antioxidant Levels in Drosophila melanogaster

2020 ◽  
pp. 27-35
Author(s):  
Chinonye A. Maduagwuna ◽  
Simeon Omale ◽  
Monday A. Etuh ◽  
Steven S. Gyang
Keyword(s):  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Methanolic Extract ◽  
Control Group ◽  
Total Protein Content ◽  
Total Thiol ◽  
Methanolic Extracts ◽  
Centre Of Excellence ◽  
Anti Oxidant ◽  
Plateau State

Aims: To investigate the anti-oxidant activity of the methanolic extracts of Caryota no seeds in Drosophila melanogaster (DM). Study Design: Experimental design. Place and Duration: Sample: African Centre of Excellence for Phytomedicine Research and Development, University of Jos, Jos Plateau State Nigeria between June 2018 and February 2019.                                                                                                                                                     Methodology: These assays were conducted by exposing 50 flies per vial to the selected concentrations (350 mg, 400 mg and 500 mg) of the extract in 5 independent replicates for seven days while control group received distilled water. The total protein content was then determined from the supernatant of the fly homogenate. The antioxidant activity and levels of GST, CAT and total thiol were then measured. The statistical difference among test groups was presumed at P < .05.                                                                                                         Results: The methanolic extract of Caryota no caused nonsignificant (P = .33) decrease in total proteins in DM below basal levels in a dose-dependent pattern.  The antioxidant activity showed nonsignificant (P = .28) lowering of the GST activity in DM below control levels. The methanolic extract of CN nonsignificantly (P > .05) increased the levels of catalase (P = .36) and total thiol levels (P = .22).                                                                                                       Conclusion: It can therefore be concluded that the methanolic extract of Caryota no contains appreciable concentrations of different types of antioxidants. This may provide perspectives for the evaluation and development of effective and safe phytomedicines created from the local biodiversity. 

scholarly journals Phytochemical characterization, anti-cancer and antimicrobial activity of isolated fractions of Alysicarpus vaginalis

2017 ◽  
Vol 12 (1) ◽  
pp. 77 ◽  
Author(s):  
Ganesh Tapadiya ◽  
Mayura A. Kale ◽  
Shweta Saboo
Keyword(s):  
Antimicrobial Activity ◽  
Cell Lines ◽  
Methanolic Extract ◽  
Hep G2 ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Anti Cancer ◽  
Proliferative Assay ◽  
Bacteria And Fungi

<p class="Abstract">The methanolic extract of <em>Alysicarpus </em>vaginalis was selected for fractionation due to its known reported biological activity. The four fractions were separated and subjected for<em> in vitro</em> antimitotic and anti-proliferative assays along with anti-cancer activity on two human cancers cell lines (SK-MEL-2 and Hep-G2). The antimicrobial potential of fractions had been evaluated against bacteria and fungi. From all fractions, acetone and n-butanol fractions were effective against the cell lines. They show strong inhibitory action with mitotic index 6.2 and 8.4 mg/mL and IC<sub>50 </sub>values of anti-proliferative assay in between 19.7 to 14.2 mg/mL respectively, which was found to be comparable to the standard methothrexate 5.9 mg/mL and 13.2 mg/mL respectively. In antimicrobial activity, the zone of inhibition had been observed in the range of 12-27 mm and MIC value was found in the range of 0.2-0.1 mg/mL. The acetone fraction was found to be most active against fungi, and<em> E. coli</em> whereas chloroform and n-butanol fractions were more effective against <em>S. aureus</em> and <em>B. </em>subtilis. The phytochemical characterization by HPLC analysis indicated the presence of important polyphenolic and steroidal compounds.</p>

scholarly journals Evaluation of Antibacterial Activity of Aqueous, Ethanolic and Methanolic Extracts of Areca Nut Fruit on Selected Bacteria

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Neda Jam ◽  
Reza Hajimohammadi ◽  
Parvin Gharbani ◽  
Ali Mehrizad
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Enterobacter Aerogenes ◽  
Areca Nut ◽  
Zone Of Inhibition ◽  
Antibacterial Effects ◽  
Fruit Extracts ◽  
Methanolic Extracts ◽  
Agar Disc

Today, the tendency to use of natural preservatives to increase food security has expanded. In the present study, antibacterial effects of Areca Nut fruit extracts were evaluated against Staphylococcus aureus, Escherichia coli, Salmonella enterica, and Enterobacter aerogenes bacteria using agar disc diffusion technique. Methanol, ethanol, and water were used as solvents for extraction by maceration method, and extracts were analyzed by GC-MS. The antibacterial activity was evaluated using microtiter broth dilution method to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results revealed that all ATCC strains were significantly inhibited by ethanolic and methanolic extracts. Escherichia coli produced a significantly larger zone of inhibition for Gentamicin ( 35 ± 0.65   mm ) and Penicillin ( 25   mm ± 0.56 ), while Enterobacter aerogenes produced smaller zone of inhibition for Gentamicin (20 ± 0.87 mm) and Penicillin ( 15 ± 0.87   mm ). Also, methanolic extract had considerable antibacterial activity with MIC value of 1.56 mg/mL against Escherichia coli. All of extracts were used to evaluate antibacterial effects in prepared cake, and as a result, all pathogenies were the most sensitive by methanolic extract in 100 mg/L of concentration except Escherichia coli that were more sensitive by ethanolic extract. In conclusion, the Areca Nut fruit extracts may be used as a natural preservative in food industries. Future studies should focus on the effect of Areca Nut fruit extracts in bakery and drinking industries.

scholarly journals Antimicrobial Activity of Opuntia cochenillifera (L.) Mill Fruit and Cladode Extracts

2016 ◽  
Vol 3 ◽  
pp. 84-89
Author(s):  
Pooja Suryawanshi ◽  
G.M. Vidyasagar
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Maximum Activity ◽  
Methanolic Extracts ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

In the present study the antimicrobial activity of chloroform and methanolic extracts of Opuntiacochenillifera for both cladode and fruits was investigated. Methanolic extract was found to be an effective against the microbes namely, E. coli,Bacillus subtilis,Staphylococcus aureus, Pseudomonas aeruginosaCandidaalbicanC.glabrataC.haemuloniiC.Tropicalis. Maximum activity was observed against E. coli, B. Subtilisand,C.albican and C.glabrata at 40mg/ml. Agar well diffusion assay was used to determine minimum inhibitory concentration of all test microorganisms.

scholarly journals Antimicrobial Activity Exerted by Total Extracts of Germander

2019 ◽  
Vol 70 (9) ◽  
pp. 3242-3244
Author(s):  
Raul Chioibas ◽  
Razvan Susan ◽  
Monica Susan ◽  
Ovidiu Mederle ◽  
Delia Berceanu Vaduva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Fungal Species ◽  
Polyphenolic Compounds ◽  
Biologically Active ◽  
E Coli ◽  
Methanolic Extracts ◽  
Teucrium Polium ◽  
Active Substances

In the present research, ethanolic and methanolic extracts of Teucrium polium (germander) were studied regarding the content of biologically active substances - in particular polyphenols - antioxidant activity and antibacterial activity. The extracts have been found to be rich in polyphenolic compounds, kaempferol and epichatechin have been found to be present in the highest concentrations. The antioxidant activity evaluated by the method of capturing the free radicals with DPPH revealed values close to those produced by the ascorbic acid. Antimicrobial activity was evaluated by the diffusimetric method and S. aureus and E. coli microorganisms were found to be the most sensitive while P. aeruginosa and fungal species were not sensitive.

scholarly journals Physicochemical, phytochemical and antibacterial studies of few medicinal plants of Visakhapatnam region against bacterial clinical isolates

2016 ◽  
Vol 5 (03) ◽  
pp. 1296
Author(s):  
Phani Kumari Uddandapu* ◽  
K. Chandrasekhara Naidu ◽  
Y. Venkateswar Rao
Keyword(s):  
Medicinal Plants ◽  
Herbal Medicines ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Anticancer Properties ◽  
Antimicrobial Efficiency ◽  
Antimicrobial Studies ◽  
Methanolic Extracts ◽  
Minimum Zone ◽  
Ethnic Uses

The present study was conducted to evaluate the physicochemical and preliminary phytochemical studies, antimicrobial studies on sixteen medicinal plants collected in and around regions of Visakhapatnam district using methanol as a solvent. Phytochemical screening revealed that methanolic extracts, contained, steroids, alkaloids, phenol, flavonoids, polyphenols and glycosides. The physicochemical parameters like ash and extractive values were determined. The highest percentage of total ash, was found in Catharanathus roseus. The extractive values can be used as a reliable aid for detecting quality of drug adulteration. Antimicrobial efficiency of studies plants was tested against four bacterial clinical pathogens including Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. The diameter of zone of inhibition (ZOI) was measured and it was found that C. roseus showed maximum zone of inhibition against gram+ve S. aureus and minimum zone of inhibition against gram–ve E. coli. Also the zone of inhibition was increased on increasing concentration. Results observed in the present study help to distinguish the plant extracts from its adulterants and quality and it may be indicative of the methanol extracts of studied plants could be a possible source to obtain new and effective herbal medicines to treat infections, which justify the ethnic uses of C. roseus against various infectious diseases apart from its anticancer properties.

scholarly journals Antimicrobial activity of leaf extracts of Euphorbia paralias L. and Melilotus sulcatus Desf. against some pathogenic microorganisms

2021 ◽  
pp. 10-15
Author(s):  
Miloud M. Miloud ◽  
Najma A. Senussi
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Methanolic Extract ◽  
Bacterial Species ◽  
Fungal Species ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Bioactive Agents ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

The present study was aimed to investigate the antimicrobial potential of leaf extracts of Euphorbia paralias and Melilotus sulcatus against four bacterial species Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella sp. and two fungal species Asperigillus niger and Aspergillus flavus. The agar well diffusion assay was used to evaluate the antimicrobial activity. The effect of these extracts was most effective against the bacterial species compared to the fungal species at a used concentration (100 mg/ml). Methanolic extracts of selected plants displayed good antimicrobial activity against all tested microorganisms species, while, no activity for aqueous extracts against tested fungal species. Methanolic extracts were the most effective plant extracts against all tested bacterial species, with MIC and MBC reached 6.2 and 12.5 mg/ml, except Klebsiella sp. which was less sensitive to M. sulcatus methanolic extract and its MIC and MBC reached 12.5 and 25 mg/ml, respectively. These plant extracts which proved to be potentially effective can be used as bioactive agents to control microorganisms caused for diseases and they can be used naturally in the human and veterinary healthcare systems.

scholarly journals In vitro study on antioxidant activity of methanolic leaf extract of Cassia fistula Linn.

2018 ◽  
Vol 7 (5) ◽  
pp. 849
Author(s):  
Bindhu R. Kamath ◽  
Sabeena Kizhedath
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanolic Extract ◽  
Heart Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Study ◽  
Cassia Fistula ◽  
Methanolic Extracts

Background: The use of medicinal plants for maintaining good health is getting attention worldwide. Antioxidants play an important role to protect damage caused by oxidative stress. In the present study methanolic extracts of Cassia fistula  was determined using DPPH for its antioxidant activity. Phytochemical investigation confirmed the presence of bioactive ingredients in the extract.Methods: The antioxidant activity of methanolic extract of Cassia fistula was evaluated using DPPH free radical assay. DPPH (1,1-diphenyl-2-picrylhydrazyine) free radical analysis is one of the accurate and frequently employed method for evaluating antioxidant activity.Results: The methanolic extracts showed increase in radical scavenging activity as concentration increases. The IC50 values were calculated for the methanolic extract. Ascorbic acid was used as control. Cassia fistula exhibited IC 50 of 79.42µg/ml.Conclusions: Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease and infectious diseases. Further evaluation of pharmacological activities of Cassia fistula may prove useful in treatment of cancer and heart diseases.

scholarly journals Antioxidant Activity, Polyphenols Content and Antimicrobial Activity of Several Native Pteridophytes of Romania

2012 ◽  
Vol 40 (1) ◽  
pp. 53 ◽  
Author(s):  
Liliana Cristina SOARE ◽  
Mariana FERDES ◽  
Stefan STEFANOV ◽  
Zapriana DENKOVA ◽  
Radosveta NICOLOVA ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Fresh Weight ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Brevibacterium Flavum ◽  
Methanolic Extracts ◽  
Cosmetic Ingredients ◽  
Better Than

The aim of this paper was to test the antioxidant activity, polyphenols content and antimicrobial activity of crude extracts obtained from leaves of pteridophyte species commonly found in Romania. The ORAC (Oxygen Radical Absorbance Capacity) of the investigated ferns varied between 421.90 ?mol TE (Trolox equivalents/g FW (fresh weight) in Dryopteris filix-mas and 128.18 ?mol TE/g FW in D. affinis. Methanolic extracts obtained from leaves of ferns have similar antioxidant activity to that of some medicinal plants. Polyphenols content in the leaves of ferns varies between 2340 mg Gallic acid equivalents (GAE)/100 g FW in D. filix-mas and 887 mg GAE/100 g FW in D. affinis. The correlation coefficient between ORAC and the total polyphenol content was R=0.985. This correlation suggests that phenolic compounds are major contributors to the antioxidant activity. The methanolic extract obtained from ferns inhibits the growth of Gram negative Escherichia coli ATCC 25922, Pseudomonas aeruginosa NBJMCC1390, Salmonella abony and Gram positive Staphyllococcus aureus ATCC 25093 and Enterococcus faecalis. The highest antimicrobial activity was determined for the Dryopteris extract. The antimicrobial activity of methanolic extracts obtained from leaves of D. filix-mas and D. affinis is better than the A. filix-femina in the case of Brevibacterium flavum ATCC 14067, Sarcina sp., Bacillus cereus ATCC 1390, Saccharomyces cerevisiae and Aspergillus niger. The tested ferns could be used as cosmetic ingredients, as preservatives in food or in antimicrobial therapy.

scholarly journals Phytochemical And Antibacterial Activityof Leave Extracts Of Guiera Senegalensis Lam On Selected Species Of Gram Positive And Gram Negative Bacteria

2013 ◽  
Vol 2 (1) ◽  
pp. 262-268 ◽  
Author(s):  
IA Mamman ◽  
MA Isa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Clinical Isolates ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Klebsiella Species ◽  
E Coli ◽  
Guiera Senegalensis

The aim of this study was to determine the preliminary phytochemical component and antibacterial activity of the leaves extract of Guiera senegalensis lam (Combretaceae) against three clinical isolates (Staphylococcus aureus, E. coli and Klebsiella species) using standard method of analysis. The test for phytochemical component revealed the presence of alkaloid, anthraquinolones, tannin and phlobatanins. The result of antibacterial activity showed that the ethanolic extract exhibit higher zone of inhibition against all the clinical isolates, with E. coli and Klebsiella species showed zone of inhibition of 35mm followed Staphylococcus aureus 30mm. Similarly, both aqueous and methanolic extract were sensitive to all the clinical isolate except Klebsiella species which showed resistance to the methanolic extract of the leaves. The results obtained in this research imply that the leaves extract of Guiera senegalensis lam could be useful in the treatment of infections caused by Staphylococcus aureus, E. coli and Klebsiella species. International Journal of Environment, Volume-2, Issue-1, Sep-Nov 2013, Pages 262-268 DOI: http://dx.doi.org/10.3126/ije.v2i1.9226

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