scholarly journals Physicochemical, phytochemical and antibacterial studies of few medicinal plants of Visakhapatnam region against bacterial clinical isolates

2016 ◽  
Vol 5 (03) ◽  
pp. 1296
Author(s):  
Phani Kumari Uddandapu* ◽  
K. Chandrasekhara Naidu ◽  
Y. Venkateswar Rao
Keyword(s):  
Medicinal Plants ◽  
Herbal Medicines ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Anticancer Properties ◽  
Antimicrobial Efficiency ◽  
Antimicrobial Studies ◽  
Methanolic Extracts ◽  
Minimum Zone ◽  
Ethnic Uses

The present study was conducted to evaluate the physicochemical and preliminary phytochemical studies, antimicrobial studies on sixteen medicinal plants collected in and around regions of Visakhapatnam district using methanol as a solvent. Phytochemical screening revealed that methanolic extracts, contained, steroids, alkaloids, phenol, flavonoids, polyphenols and glycosides. The physicochemical parameters like ash and extractive values were determined. The highest percentage of total ash, was found in Catharanathus roseus. The extractive values can be used as a reliable aid for detecting quality of drug adulteration. Antimicrobial efficiency of studies plants was tested against four bacterial clinical pathogens including Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. The diameter of zone of inhibition (ZOI) was measured and it was found that C. roseus showed maximum zone of inhibition against gram+ve S. aureus and minimum zone of inhibition against gram–ve E. coli. Also the zone of inhibition was increased on increasing concentration. Results observed in the present study help to distinguish the plant extracts from its adulterants and quality and it may be indicative of the methanol extracts of studied plants could be a possible source to obtain new and effective herbal medicines to treat infections, which justify the ethnic uses of C. roseus against various infectious diseases apart from its anticancer properties.

scholarly journals Studies on effects of different solvent extracted samples of O. sanctum (Basil) stem on the growth of S. typhi

2020 ◽  
Vol 26 (2) ◽  
Author(s):  
Seema Kumari ◽  
Vinod Prasad
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Herbal Medicines ◽  
Water Extract ◽  
Salmonella Typhi ◽  
Hot Water ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Hot Water Extract ◽  
Minimum Zone

Typhoid is a problematic fever, caused by Salmonella typhi. Present investigation is based upon studies of antibacterial effects of different solvent extracted samples of O. sanctum stem on growth of S. typhi. The crude extracts of stem of O. sanctum were prepared from three different solvents such as methanol, ethyl acetate and hot water. Four different concentrations (25%, 50%, 75% and100%) were prepared from each one of the solvent extracted sample. Agar well diffusion method was used to test the antibacterial activity of different solvent extracted samples of O. sanctum stem. Analysis of data indicates that a metabolic extract of O. sanctum stem shows maximum zone of inhibition and it was of 26mm at 100% concentration and minimum zone of inhibition was of 19mm at 50% concentration. Ethyl acetate extract of O. sanctum stem shows maximum zone of inhibition and it was of 29mm at 100% concentration and minimum was of 14mm at 25% concentration. Hot water extract of O. sanctum stem shows maximum zone of inhibition and it was of 28mm at 100% concentration and minimum zone of inhibition was of 12mm at 25% concentration. This study indicates that ethyl acetate extract of O. sanctum stem is more effective against S. typhi as compared to methanol and hot water extracted solvent. Contrary to the antibiotics or synthetic medicine associated with many side effects, this plant part can be used as a herbal medicines against Salmonella typhi infection.

scholarly journals Investigating In Vitro Antibacterial Activities of Medicinal Plants Having Folkloric Repute in Ethiopian Traditional Medicine

2019 ◽  
Vol 24 ◽  
pp. 2515690X1988627 ◽  
Author(s):  
Mekonnen Sisay ◽  
Negussie Bussa ◽  
Tigist Gashaw ◽  
Getnet Mengistu
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Antimicrobial Agents ◽  
Bacterial Isolates ◽  
Zone Of Inhibition ◽  
Traditional Use ◽  
Gram Negative ◽  
E Coli ◽  
Slow Development

Medicinal plants are targeted in the search for new antimicrobial agents. Nowadays, there is an alarmingly increasing antimicrobial resistance to available agents with a very slow development of new antimicrobials. It is, therefore, necessary to extensively search for new agents based on the traditional use of herbal medicines as potential source. The antibacterial activity of 80% methanol extracts of the leaves of Verbena officinalis (Vo-80ME), Myrtus communis (Mc-80ME), and Melilotus elegans (Me-80ME) was tested against 6 bacterial isolates using agar well diffusion technique. In each extract, 3 concentrations of 10, 20, and 40 mg/well were tested for each bacterium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. Vo-80ME and Mc-80ME exhibited promising antibacterial activity against Staphylococcus aureus with the highest zone of inhibition being 18.67 and 26.16 mm, respectively at concentration of 40 mg/well. Regarding gram-negative bacteria, Vo-80ME exhibited an appreciable activity against Escherichia coli and Salmonella typhi. Mc-80ME displayed remarkable activity against all isolates including Pseudomonas aeruginosa with the maximum zone of inhibition being 22.83 mm. Me-80ME exhibited better antibacterial activity against E coli, but its secondary metabolites had little or no activity against other gram-negative isolates. The MIC values of Vo-80ME ranged from 0.16 to 4.00 mg/mL. The lowest MIC was observed in Mc-80ME, with the value being 0.032 mg/mL. Mc-80ME had bactericidal activity against all tested bacterial isolates. Mc-80ME showed remarkable zone of inhibitions in all tested bacterial isolates. Besides, Vo-80ME showed good antibacterial activity against S aureus, E coli, and S typhi. Conversely, Me-80ME has shown good activity against E coli only. Generally, M communis L and V officinalis have good MIC and MBC results.

scholarly journals Antimicrobial and anti-oxidant potential of Periploca hydaspidis

2015 ◽  
Vol 10 (3) ◽  
pp. 645 ◽  
Author(s):  
Rafi Ullah ◽  
Jehan Bakht ◽  
Mohammad Shafi
Keyword(s):  
Antioxidant Activity ◽  
Growth Inhibition ◽  
Methanolic Extract ◽  
Maximum Growth ◽  
Zone Of Inhibition ◽  
Aqueous Fraction ◽  
E Coli ◽  
Methanolic Extracts ◽  
Anti Oxidant ◽  
Diffusion Assay

<p>The present study investigates antimicrobial and anti-oxidant potential of different solvent extracted samples from <em>Periploca hydaspidis </em>through disc diffusion assay. The data revealed that all the extracts reduced the growth of the tested microbes using three different concentrations. <em>Pseudomonas aeruginosa </em>and <em>E. coli</em> were sensitive to crude extracts and all fractions measuring varying degree of growth inhibition.  Similarly, the growth of <em>Citrobacter freundii</em> was not inhibited by crude methanolic extracts, n-hexane, ethyl acetate and aqueous extracted samples. Maximum growth inhibition was measured against <em>Klebsiella pneumoniae</em> exposed to aqueous fraction followed by n-hexane fraction. Our results also suggested that among various fungi, <em>Candida albicans</em> was sensitive to crude methanolic extracts, n-hexane and aqueous fractions compared with other fractions. The rest of the fungi under test were resistant to crude and all fractions showing no zone of inhibition. All the extracted samples of the stems and roots showed antioxidant activity, however, crude methanolic extract of stem tissues exhibited better antioxidant activity than the other extracts.</p>

scholarly journals Phytochemical and Antimicrobial Studies of the Crude Extracts of the Leaves of Carica papaya Linn (Pawpaw) and Psidium guajava Linn (Guava)

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Priscilla Alexander ◽  
Ismaila Yada Sudi ◽  
Martin Tizhe
Keyword(s):  
Antibacterial Activity ◽  
Carica Papaya ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Psidium Guajava ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Active Inhibition ◽  
Antimicrobial Studies ◽  
Alternative Antibiotic

Psidium Guajava (Guava) and Carica Papaya leaves which have some ethnomedicinal applications were investigated. Phytochemical screening of their leaves revealed the presence of flavonoids, saponins, terpenoids, steroids, tannins and glycosides. Antimicrobial screening of the crude ethanolic extracts showed activity against Staphylococcus aureus, Streptococcus faecalis, and Escherichia coli. The minimum inhibitory concentration (MIC) for P. gujava on the organism was found to be 5.00 mg/ml against S. aureus, E. coli and S. faecalis, while that of C. papaya leaves is 10.00 mg/ml against S. aureus, E. coli and 8.00 mg/ml against S. faecalis respectively. C. papaya ethanolic extract showed more active inhibition against S. aureus with mean zone inhibition of 9.54 ± 0.03.  P.   gujava ethanolic extract has more active inhibition against E. coli with antibacterial activity with mean zone of inhibition of 10.44±0.02 and S. faecalis with mean zone of inhibition of 6.72 ± 0.01 respectively.  This study showed that the leaves extract of these plants are good sources of bioactive compounds. Demonstration of antibacterial activity against the test isolates is an indication that there is possibility of sourcing alternative antibiotic substances in these plants for the production of newer antibacterial agents. These plants therefore, could be an important source of medicine for the treatment of various diseases.

scholarly journals In Vitro comparison in the antimicrobial effect between Ciprofloxacin and Neem leaf extract (Azadirachta indica) on Escherichia coli Growth

2016 ◽  
Vol 15 (2) ◽  
pp. 172-177
Author(s):  
Quazi Rubyath Banna ◽  
Badar Uddin Umar ◽  
S M Niazur Rahman ◽  
Tanbira Alam ◽  
Kazi Selim Anwar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Medicinal Plants ◽  
Medical Science ◽  
Antibiotic Sensitivity ◽  
Antimicrobial Effect ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Neem Leaves ◽  
Wide Range

Objective: Medicinal plants remain in vogue to treat some diseases in lower socio-economic communities, despite the availability of antimicrobials, often. Majority of rural Bangladeshi and tribal people being grossly illiterate and ignorant, use various herbs to treat a wide range of diseases. Of several medicinal-plants, neem is reported to have enormous impact in treating inflammation and infections. We, therefore, compared the antimicrobial effect of ethanolic neem leave extract (ENLE) on Escherichia coli (E. coli), with that of Ciprofloxacin. Materials & Methods: This experimental study compared the in vitro antimicrobial activity between ENLE and Ciprofloxacin on E. coli carried out in Department of Pharmacology and Therapeutics of SS-Medical College, Dhaka, Bangladesh. Antimicrobial efficacy of ENLE and ciprofloxacin (5µg; Oxoid, UK) was determined against E. coli following minimum inhibitory concentration. By filtration and evaporation of Neem leaves ENLE was prepared. Antibiotic Sensitivity Test was performed on Muller-Hinton agar using a twofold serial dilution. Results: ENLE showed an inhibitory effect on the growth of E. coli at the concentration of 3.125 mg/ml. Antibacterial susceptibility of E. coli was performed on MHA and diameters of zone of inhibition by both ENLE and Ciprofloxacin were measured after overnight aerobic incubation at 37°C. Diameter of zone of inhibition against E. coli was 28 ± 0.16 mm with ENLE, 36 ± 0.07 mm with Ciprofloxacin (5µg/disk) (p<0.000). Conclusion: Findings of this preliminary in-vitro experiment though suggests that, ENLE against E. coli showed limited efficacy, better efficacy of Ciprofloxacin cannot be ruled out unless further in depth studies elucidates stronger evidences to support it.Bangladesh Journal of Medical Science Vol.15(2) 2016 p.172-177

scholarly journals Antimicrobial Evaluation of Natural Spices (Tumeric, Garlic, Onion, Celery and Cinnamon) Versus Synthetic Antibiotics (Tetracycline, Clindamycin and Ceftriazone) at Three Different Concentrations (10%, 50% and 100%)

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
RC Jagessar ◽  
F Ali ◽  
G Shrikant ◽  
M Astwood
Keyword(s):  
Herbal Medicines ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Synthetic Drugs ◽  
Microbial Inhibition ◽  
A Value ◽  
Antimicrobial Evaluation ◽  
Aseptic Conditions ◽  
The Cost ◽  
Diffusion Assay

Most Synthetic drugs have several side effects when administered and the cost of synthesizing them is an expensive endeavour. One alternative is to use herbal medicines. Guyana has an abundance of diverse flora which has been receiving attention in this area. However, much more research needs to be done. The antibacterial activity of natural spices (Tumeric, garlic, onion, celery and cinnamon) was investigated against E. coli, S. aureus, K. pneumoniae and P. aeruginosa and was compared with synthetic antibiotics (Tetracycline, Clindamycin and Ceftriazone) at three different concentrations of 10%, 50% and 100%. The Disc Diffusion Assay was used to investigate the antimicrobial potency of the synthetic drugs and herbal medicines under aseptic conditions. The synthetic drugs were prepared at different concentrations of 10%, 50% and 100%, whereas the extract was investigated at 100% concentration. Experiments were done in triplicates and the diameter of zone of microbial inhibition (DZOI) was measured and expressed as the mean with standard deviation (SD). The precision of comparisons (e.g. 95% confidence intervals) and Area of Zone of Inhibition, AZOI was also computed. Selected microorganisms were also tested against a reference standard antibiotic, Ampicillin and Nystatin. For the synthetic drugs, the highest Area of Zone of Inhibition, AZOI of 1566.4 mm2 was induced by Clindamycin against S. aureus at 50% concentration whereas the lowest AZOI of 245.09 mm2 was induced by P. auriginosa at 10% concentration. For the herbal extracts, the highest AZOI of 1256.6 mm2 was induced by the onion extract versus K. pneumonia whereas the lowest AZOI of 50.3 mm2 was induced by the Tumeric extract against E. coli. Antimicrobial selectivity was also observed for both synthetic and herbal medicines. For example, for tetracycline against S. aureus, AZOI of 1093.92 mm2 was obtained whereas against P. auriginosa, AZOI of 530.67 mm2 was observed. Garlic exhibited AZOI of 139.49 mm2 against E. coli whereas a value of 78.5 mm2 was obtained against P. aeruginosa.  

scholarly journals Anti-Bacterial Analysis of Some Herbal Medicines in Mbale, Eastern Uganda

2020 ◽  
pp. 28-33
Author(s):  
H. A. Aliyu ◽  
A. M. Danjuma ◽  
K. Abubakar
Keyword(s):  
Escherichia Coli ◽  
Herbal Medicine ◽  
Random Sampling ◽  
Herbal Medicines ◽  
Antibacterial Activities ◽  
Bacterial Activity ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Eastern Uganda

This study was aimed at investigating the anti-bacterial activity of some on shelf herbal medicines for treatment of Cholera and Typhoid in markets. The sample was collected from shops that sell herbal medicines using random sampling to investigate the effectiveness of these herbal medicines on Escherichia coli and Salmonella enterica serotype typhi. It was noticed that all the four herbal medicines that were subjected to antibacterial activities using E. coli and S. typhi, showed effect on the bacteria. Diameters of zones of inhibition was measured, which showed that zone of inhibition varies among the on shelf herbal medicines. Serial dilution was also carried out to check for the effect of concentration. Herbal Medicine for Typhoid 1 showed the highest zone of inhibition 14.36 mm and the lowest zone of inhibition was Herbal Medicine for Cholera 1 with 10.17mm on S. Typhi. While for E. coli, the highest zone of inhibition was 12.83mm shown by Herbal Medicine for Cholera 2 and the lowest was 10.25mm shown by Herbal Medicine for Typhoid 1.

scholarly journals Medicinal Plants and Mushrooms with Immunomodulatory and Anticancer Properties—A Review on Hong Kong’s Experience

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2173
Author(s):  
Grace Gar-Lee Yue ◽  
Clara Bik-San Lau ◽  
Ping-Chung Leung
Keyword(s):  
Clinical Trials ◽  
Medicinal Plants ◽  
Infection Control ◽  
Immune Modulation ◽  
Evidence Based ◽  
Medicinal Mushroom ◽  
Immunomodulatory Effects ◽  
Anticancer Properties ◽  
Further Development ◽  
Insight Into

The immune modulating effects of selected herbs deserve careful studies to gain evidence-based support for their further development. We have been working hard on many items of medicinal herbs to gain insight into their immunomodulatory effects relevant to cancer treatment in particular, while infection control is not excluded. Nine of them have been selected to give the results of our exploration on their biological, particularly immunomodulatory activities. Since Hong Kong people especially favor one medicinal mushroom, viz. Coriolus versicolor, a number of clinical trials using Coriolus for cancer-related studies are included in this review. While immune modulation platforms are being built for relevant studies, a brief account on the research targets and related procedures are given.

Electrospun PU/MgO/Ag Nanofibers for Antibacterial Activity and Flame Retardency

2021 ◽  
pp. 0887302X2110094
Author(s):  
V. Mamtha ◽  
H. N. Narasimha Murthy ◽  
V. Pujith Raj ◽  
Prashantha Tejas ◽  
C. S. Puneet ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Melting Point ◽  
Protective Clothing ◽  
Electrospun Nanofibers ◽  
Synthesis And Characterization ◽  
Zone Of Inhibition ◽  
Solution Combustion ◽  
E Coli ◽  
Afterglow Time

Antibacterial activity and fire retardation are equally desired for protective clothing. For achieving this, AgNP and MgO are independently researched as nanofillers in Polyurethane based electrospun nanofibers and their synergistic effect is scarcely addressed. This article reports synthesis and characterization of MgO of 70.01 nm and AgNP of 51 to 76 nm by solution combustion and hydrothermal routes respectively and their incorporation in electrospinning of Polyurethane. Flow rate 1 ml/hr, applied voltage 13 kV, tip to collector distance 15 cm were adopted for the electrospinning. Nanofibers of 65 nm were obtained for PU/MgO (3 wt. %) and 106 nm for PU/MgO (3 wt. %)/Ag (1 wt. %). Addition of MgO increased the melting point, after flame time and afterglow time. Incorporation of AgNP improved antibacterial activity. PU/MgO/Ag (2 wt. %) exhibited zone of inhibition of 2.1 cm and 3 cm against E. Coli and S. Aureus, respectively.

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