scholarly journals Beneficial Effects of Combination Therapy of Sitagliptin and β-carotene Drugs on Streptozotocin-induced Diabetic Rats

2020 ◽  
Vol 23 (2) ◽  
pp. 87-95
Author(s):  
Pulye Roy ◽  
Monirul Islam ◽  
Mohammad Saiful Islam ◽  
Mamunur Rashid
Keyword(s):  
Combination Therapy ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Fixed Dose ◽  
Diabetic Patients ◽  
Control Groups ◽  
Chronic Hyperglycemia ◽  
Serum Glutamate ◽  
Β Carotene

Diabetes mellitus (DM), characterized by chronic hyperglycemia is one of the most common chronic diseases affecting millions of people worldwide. A significant component of the risk associated with DM is thought to be because of its abnormal lipid ‘triad’ profile. All the associated complications are mainly due to imbalance between generation of free radicals and the antioxidant systems like catalase (CAT) and super oxide dismutase (SOD) enzymes. Moreover, increased level of serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) are considered a consequence of hepatocyte damage. The current study was designed to investigate the antihyperglycemic and antidyslipidemic effects, oxidative stress and liver dysfunction indices of fixed dose combination therapy of sitagliptin [50 mg/70kg body weight (BW)] and β-carotene (5 mg/70kg BW) on streptozotocin (45 mg/kg BW) induced diabetic rats (SIDRs). The study showed that combination therapy induced a significant decrease in blood glucose level (BGL) from 19.09 ± 0.13 to 6.86 ± 0.11 mmol/l in comparison to the control group after daily treatment for three weeks. In case of dyslipidimic effect, combination therapy reduced total cholesterol (TC) (22.96%), triglycerides (TG) (17.54%) and low-density lipoprotein (LDL)-cholesterol (52.25%) levels significantly and increased high-density lipoprotein (HDL)-cholesterol level (55.98%) in comparison with respective diabetic control groups. We also observed that combination therapy lowered SGPT & SGOT level by 46.39% and 37.23%, respectively and increased SOD & CAT enzyme activity by 63.79% and 48.32%, respectively in comparison with diabetic control groups. These changes were significantly better than those of sitagliptin and β-carotene monotherapy. The suggestions of our findings of this research are that combination therapy is more potent than their individual monotherapy and may be beneficial for the treatment of diabetic patients with associated complications. Bangladesh Pharmaceutical Journal 23(2): 87-95, 2020

scholarly journals Evaluation of Sclerorhachis leptoclada hydroalcoholic extract on glucose and cholesterol levels, triglyceride, Serum HDL and LDL in male diabetic rats

2020 ◽  
pp. 344-354
Keyword(s):  
Group Treatment ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
The Body ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Diabetic Patients ◽  
Hydroalcoholic Extract

Background and Aim: Diabetes is a metabolic disorder in the body. Sclerorhachis leptoclada belongs to the chicory family, is used to treat aids and cancer due to the antioxidant compounds in the root. Therefore, in this study, the effect of hydroalcoholic extract of Sclerorhachis leptoclada on glucose and cholesterol, triglyceride, high density lipoprotein (HDL) and low density lipoprotein (LDL) levels in diabetic rats was investigated. Materials and Methods: In this experimental study, 60 male Wistar rats were randomly divided into 10 groups of 6: Control group, treatment groups 1, 2, and 3 (healthy, treated with 150 mg/kg and 300 and 600 extracts), Diabetic control group, experimental groups 1, 2 and 3 (diabetic, treated with doses of 150 mg/kg and 300 and 600 extracts), Positive control group (diabetic+glibenclamide), The healthy group (healthy+glibenclamide) was divided. After one month of gavage, blood samples were taken from the rats, and glucose, urea, creatinine, and albumin levels were measured. A comparison was made between the effect of hydroalcoholic extract of Sclerorhachis leptoclada and the common drug glibenclamide and the results of the groups were compared by using t-test and ANOVA. Results: The results showed that the administration of hydroalcoholic extract of Sclerorhachis leptoclada can significantly reduce glucose levels in experimental groups (diabetic+extract at doses of 150, 300, 600) compared with the diabetic group (p<0.001) Also, the consumption of hydroalcoholic extract of Sclerorhachis leptoclada in diabetic patients causes a significant reduction in triglyceride, HDL and LDL levels in diabetic rats and has hypoglycemic effects. Conclusion: The results of this study showed that the consumption of hydroalcoholic extract of Sclerorhachis leptoclada in diabetic rats significantly reduces serum levels of glucose, triglyceride and creatinine in diabetic rats and has hypoglycemic effects

scholarly journals Synergistic Effect of β-Carotene and Metformin on Antihyperglycemic and Antidyslipidemic Activities in Streptozotocin-Induced Diabetic Rats

2021 ◽  
pp. 79-87
Author(s):  
Khadiza Khanam ◽  
Sultana Rajia ◽  
Mim Yeasmin ◽  
Munira Morshed ◽  
Rashidul Haque
Keyword(s):  
Combination Therapy ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Biological Properties ◽  
Albino Rats ◽  
Protective Effects ◽  
Antidiabetic Therapy ◽  
Β Carotene

Purpose: Worldwide prevalence of diabetes mellitus (DM) has become an issue of great concern in current decades. To date, a large number of biological properties have been reported from carotenoids, particularly protective effects against cancer, cardiovascular diseases, and DM, including enhancement of insulin sensitivity.In this study, we aimed to evaluate the efficacy of β-carotene as an additive agent with metformin in ameliorating Type2 (T2)DM. Methods: In this experiment, fasting blood glucose level (BGL), low density lipoprotein (LDL), high density lipoprotein (HDL), total cholesterol (TC) and triglycerides (TG) were measured in serum of Wister albino rats with streptozotocin (STZ)-induced diabetes and after treatment with metformin (850mg/70kg b.w.) and β-carotene (10 mg/70kg b.w.) administered orally once daily for three weeks. Results: Metformin and β-carotene treatments individually resulted in significant (p<0.001) reversal of the diabetes induced increase in BGL, LDL, TC and TG, whereas significantly increased the STZ-induced decrease in HDL, compared to diabetic control. As compared to the monotherapy, the combination therapy with metformin and β-carotene showed a significant (p<0.001) attenuation of BGL and serum level of LDL, TC, and TG and a slight increase (p<0.05) in serum HDL level, as compared to the treatment with β-carotene, but not with metformin. Conclusion: The combination therapy of β-carotene and metformin produced a significant antidiabetic and antihyperlipidemic effect than the monotherapy alone and provides a scientific rationale for their use in antidiabetic therapy as a potential antioxidant.

scholarly journals Combination Therapy of Momordica charantia Linn. (Bitter Melon) with Antihyperglycemic Agent Gives Increased Antioxidative and Hepatoprotective Actions in Alloxan-induced Diabetic Rats

2019 ◽  
Vol 22 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Monirul Islam ◽  
Md Saiful Islam ◽  
Shaheda Zannah ◽  
Mamunur Rashid
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Combination Therapy ◽  
Diabetic Rats ◽  
Momordica Charantia ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Bitter Melon ◽  
Dose Combination ◽  
Serum Glutamate

Bitter melon, a popular vegetable of the Asian and African community, is used as an antidiabetic herb for the management of diabetes and its associated complications. The fastest growing metabolic disorder, diabetes mellitus (DM), is now a major cause of morbidity and mortality in developed countries. All the associated complications produced in diabetes are mainly caused by the imbalance between generation of free radicals and the antioxidant systems like catalase (CAT) and super oxide dismutase (SOD) enzymes. Again increased level of serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) are considered a consequence of hepatocyte damage due to non-alcoholic fatty liver disease (NAFLD) and patients with diabetes have an increased risk NAFLD and steatohepatitis. The present study was aimed at evaluating the effects of fixed dose combination therapy of Momordica charantia crude methanolic extract with metformin on oxidative stress and liver dysfunction indices in alloxan-induced diabetic Swiss Albino rats. Various groups of alloxan-induced diabetic rats (AIDRs) were daily treated for four weeks with bitter melon extract (BME) [(300 mg/kg body weight (BW)], metformin [(15 mg/kg body weight (BW)] and their combination dose (150 mg/kg BW + 7.5 mg/kg BW), separately through oral gavage. In our study, we observed that combination therapy lowered SGPT & SGOT level by 34.58% and 47.89%, respectively and increased SOD & CAT enzyme activity by 74.94% and 43.36%, respectively in comparison with their diabetic control groups. The changes obtained from the combination therapy were significantly (p<0.05) better than those of metformin and BME therapy alone. These findings suggest that this fixed dose combination therapy is more potent than their individual monotherapy and diabetic patients with increased oxidative stress and liver complications may be benefited by using this treatment. Bangladesh Pharmaceutical Journal 22(1): 34-40, 2019

KELULUT HONEY SUPPLEMENTATION PREVENTS SPERM AND TESTICULAR OXIDATIVE DAMAGE IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

2017 ◽  
Vol 79 (3) ◽  
Author(s):  
Siti Balkis Budin ◽  
Fatin Farhana Jubaidi ◽  
Siti Nur Farahana Mohd Noor Azam ◽  
Nur Liyana Mohamed Yusof ◽  
Izatus Shima Taib ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Sperm Quality ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sod Activity ◽  
Male Adult ◽  
Pathological Conditions ◽  
Sprague Dawley Rats

Previous studies found that Kelulut Honey produced by Trigona spp. bees is able to prevent oxidative damage in various pathological conditions.  Thus, the present study aimed to determine whether Kelulut Honey could prevent the sperm and testicular damage in streptozotocin-induced diabetic rats. Male Adult male Sprague-Dawley rats were divided into four groups: Non-Diabetic (NDM), Non-Diabetic with Kelulut Honey supplementation (NDMKH), Diabetic without supplementation (DM) and Diabetic with Kelulut Honey supplementation (DMKH).  Kelulut honey was given at the dose of 2.0 g/kg weight daily via gavage for 28 consecutive days. Results showed that sperm quality produced by diabetic rats supplemented with Kelulut honey significantly improved compared to the diabetic control groups (p<0.05). SOD activity and GSH level increased significantly (p<0.05) whereas PC and MDA levels significantly decreased in sperm and testis of DMKH rats when compared to DM rats (p<0.05). Histological observation showed obvious increase in spermatozoa in the lumen of epididymis and increased spermatogenic cells density in the testis of DMKH group.  In conclusion, Kelulut Honey has a potential in preventing the damage of sperm and testis in diabetic rats.

Ultrastructural Analysis of In Vivo Hypoglycemiant Effect of Two Polyoxometalates in Rats with Streptozotocin-Induced Diabetes

2015 ◽  
Vol 21 (5) ◽  
pp. 1236-1248 ◽  
Author(s):  
Ştefana Bâlici ◽  
Modeste Wankeu-Nya ◽  
Dan Rusu ◽  
Gheorghe Z. Nicula ◽  
Mariana Rusu ◽  
...  
Keyword(s):  
Diabetic Control ◽  
Diabetic Rats ◽  
Ultrastructural Analysis ◽  
Control Groups ◽  
Insulin Synthesis ◽  
Self Assembling ◽  
Insulin Granules ◽  
Streptozotocin Induced Diabetes ◽  
The Mean

AbstractTwo polyoxometalates (POMs), synthesized through a self-assembling method, were used in the treatment of streptozotocin (STZ)-induced diabetic rats. One of these nanocompounds [tris(vanadyl)-substituted tungsto-antimonate(III)-anions—POM1] was previously described in the literature, whereas the second [tris-butyltin-21-tungsto-9-antimonate(III)-anions—POM2], was prepared by us based on our original formula. In rats with STZ-induced diabetes treated with POMs (up to a cumulative dose of 4 mg/kg bodyweight at the end of the treatments), statistically significant reduced levels of blood glucose were measured after 3 weeks, as compared with the diabetic control groups (DCGs). Ultrastructural analysis of pancreatic β-cells (including the mean diameter of secretory vesicles and of their insulin granules) in the treated diabetic rats proved the POMs contribute to limitation of cellular degeneration triggered by STZ, as well as to the presence of increased amounts of insulin-containing vesicles as compared with the DCG. The two POMs also showed hepatoprotective properties when ultrastructural aspects of hepatocytes in the experimental groups of rats were studied. Based on our in vivo studies, we concluded that the two POMs tested achieved hypoglycemiant effects by preventing STZ-triggered apoptosis of pancreatic β-cells and stimulation of insulin synthesis.

Altered response in renal blood flow and oxygen tension to contrast media in diabetic rats

2003 ◽  
Vol 44 (3) ◽  
pp. 347-353 ◽  
Author(s):  
F. Palm ◽  
P.-O. Carlsson ◽  
P. Hansell ◽  
O. Hellberg ◽  
A. Nygren ◽  
...  
Keyword(s):  
Blood Flow ◽  
Oxygen Tension ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Medullary Blood Flow ◽  
Streptozotocin Diabetic Rats ◽  
Altered Response

Purpose: To investigate the effect of the contrast medium (CM) iopromide on renal microcirculation and oxygen tension in non-diabetic control and streptozotocin-diabetic Wistar Furth rats. Materials and Methods: Oxygen tension was measured with Clark-type microelectrodes and blood flow with laser-Doppler flow probes. In order to differentiate between an acutely increased blood glucose concentration and a long-term diabetic state, some of the non-diabetic control rats were intravenously infused with glucose. Results: CM decreased the medullary oxygen tension in control (non-diabetic normoglycemic) rats (∼35%) but not in diabetic rats. Medullary blood flow in control rats increased after CM administration and remained elevated, while it was unchanged in the diabetic rats. In response to CM, glucose-infused control rats responded similarly to control animals in medullary oxygen tension, but similarly to diabetic rats in medullary blood flow. Contrary to in control rats, medullary oxygen tension was unchanged in diabetic animals after CM administration. Conclusion: Streptozotocin-diabetic rats have an altered response to intravenous injection of the CM iopromide compared to non-diabetic rats. The unaltered medullary oxygen tension, seen in the diabetic group after injection of CM, suggests that non-hemodynamic mechanisms are responsible for the increased frequency of renal failure commonly seen among diabetic patients.

scholarly journals Evaluation of Antihyperlipidaemic and Antioxidant Activity of Astercantha longifolia (Linn.) Nees and Pergularia daemia (Forsskal) Chiov

2020 ◽  
pp. 134-138
Author(s):  
T. P. Kumari Pushpa Rani ◽  
A. Doss ◽  
S. P. Anand
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Methanol Extract ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol

Objective: To evaluate the antioxidant and antihyperlipidaemic effect of methanol extracts of Asteracantha longifolia and Pergularia daemia leaf in alloxan induced diabetic rats. Methods: Swiss albino rats were made diabetic by a single dose of alloxan monohydrate (150 mg/kg i.p.). Blood glucose levels and body weights of rats were measured using on weekly intervals i.e day 0,7,14 and 21 after daily administration of both extracts at dose 200 mg/kg bw. Other biochemical parameters such as serum cholesterol, triglycerides, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, phospholipids and serum protein, albumin, globulin levels were also measured at the end of study. The antioxidant enzymes (CAT, SOD & GSH) were also measured in the diabetic rats. Results: In the acute toxicity study, methanol extract of both plants were non-toxic at 2000 mg/kg in rats. The increased body weight, decreased blood glucose and other biochemical parameters level were observed in diabetic rats treated with methanol extracts of both plants compared to diabetic control rats. The antioxidant activity of both plant extracts were also exhibited significant activity. In diabetic rats, methanol extract of both plants administration, altered lipid profiles were reversed to near normal than diabetic control rats. Conclusions: Methanol extract of both plants (Asteracantha longifolia and Pergularia daemia) leaf possesses significant antioxidant and antihyperlipidaemic activity in diabetic rats.

scholarly journals Myrmecodia platytyrea Methanol Tuber Extract Ameliorates Hyperglycemia In STZ-Induced Diabetic Sprague-Dawley Male Rats

2021 ◽  
pp. 338-348
Author(s):  
Mizaton Hazizul Hasan ◽  
Hasbullani Zakaria ◽  
Ibtisam Abdul Wahab ◽  
Thellie Ponto ◽  
Aishah Adam
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Male Rats ◽  
Current Therapy ◽  
Diabetic Patients ◽  
Sprague Dawley ◽  
Tuber Extract

Type 2 diabetes mellitus (T2DM) is one of the main non-communicable chronic diseases that has many complications that compromise the quality of life. Hence, the need to find alternatives to replace the current therapy or as an adjuvant. Tubers of Myrmecodia platytytrea (Rubiaceae) has been used traditionally as an alternative therapy for the management of cancer and other inflammatory-related disorders. The aim of this study was to investigate the potency of M. platytytrea methanolic tuber extract (MPMTE) as an antihyperglycemic agent, in vivo. :The streptozotocin (STZ)-induced diabetic rats were treated orally with MPMTE (100, 200 and 400 mg/kg) and metformin (positive control, 100 mg/kg) daily for 14 days. Blood glucose level and other biochemistry analysis were conducted including histological examination on liver, kidney and pancreas.  The STZ-induced diabetic rats treated with MPMTE (200 and 400 mg/kg) had significant decreased (p<0.05) in fasting blood glucose, total cholesterol, triglycerides and low-density lipoprotein (LDL) with no significant changes in high-density lipoprotein (HDL) compared to STZ-induced untreated diabetic rats. Liver, kidney and pancreas were devoid of any damage caused by STZ.  MPMTE had strong antihyperglycaemic activity and was protective against any STZ-induced organ damage. Thus, MPMTE can be further developed into an adjuvant therapy for diabetic patients.

scholarly journals Blood Lipids and Its Atherogenic Indices in Alloxan Induced Diabetic Male Rats

2020 ◽  
pp. 25-33
Author(s):  
O. D. Chikezie ◽  
S. C. Meludu ◽  
I. S. I. Ogbu ◽  
B. N. Egejuru ◽  
T. Ude ◽  
...  
Keyword(s):  
Blood Lipids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Low Density Lipoprotein Cholesterol ◽  
Atherogenic Indices ◽  
Diabetes Induction

Background: Diabetes mellitus is a group of metabolic disorders which result to excessive accumulation of blood sugar over a prolonged period. Due to higher risk of diabetes mellitus to cardiovascular disease, it is crucial to identify and address these cardiovascular risks. This study assessed the effects of diabetes on levels of some blood lipids and its atherogenic indices in diabetic male rats. Methods: This is an experimental study that involved 40 apparently healthy adult male albino rats (wistar strain) which were randomly assigned to five groups (A, B, C, D and E) of eight (8) animals each. Group A (Normal Control of No intervention for 72 hours), Group B (Diabetic rats of 72 hours post diabetes induction), Group C (metformin treated diabetic rats), Group D (Diabetic Control untreated) and Group E (Normal Control of 3 weeks post diabetes induction). Seven milliliters of fasting blood sample were collected from all the subjects. Serum levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c) and very low density lipoprotein cholesterol (VLDL-c) were determined using standard methods. Atherogenic indices, non HDL cholesterol (Non HDL-c), cardio risk ratio (CRR), atherogenic index of plasma (AIP), atherogenic coefficient (AC) and atherosclerosis index (AI) were calculated. It was analyzed statistically using SPSS version 23.0. Results: The mean values of HDL-c was significantly higher in the treated diabetic group when compared with untreated diabetic control (P<0.05) while TC, TG, LDL-c, VLDL-c, Non HDL-c, CRR, AIP, AC and AI were significantly lower in treated diabetics when compared to the untreated diabetic control (P<0.05). Also, blood mean levels of HDL-c were significantly lower in the diabetic groups (treated and untreated) when compared with non diabetic control (P<0.05) while TC, TG, LDL-c, VLDL-c, Non HDL-c, CRR, AIP, AC and AI were significantly higher in the diabetic groups (treated and untreated) when compared with non diabetic control (P<0.05). Conclusion: The study suggests that atherogenic indices can serve as predictive pointer for future cardiovascular event especially, when LDLc value is normal. Also hyperglycemia could cause significant alterations of lipids, but metformin treatment has showed not only hypoglycemic effect, but also anti-hyperlipidemic properties.

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