Prenylated Flavonoids and C-15 Isoprenoid Analogues with Antibacterial Properties from the Whole Plant of Imperata cylindrica (L.) Raeusch (Gramineae)

The local botanical Imperata cylindrica in Cameroon was investigated for its antibacterial potency. The methanol extract afforded a total of seven compounds, including five hitherto unreported compounds comprising three flavonoids (1–3) and two C-15 isoprenoid analogues (4 and 5) together with known derivatives (6 and 7). The novelty of the flavonoids was related to the presence of both methyl and prenyl groups. The potential origin of the methyl in the flavonoids is discussed, as well as the chemophenetic significance of our findings. Isolation was performed over repeated silica gel and Sephadex LH-20 column chromatography and the structures were elucidated by (NMR and MS). The crude methanol extract and isolated compounds showed considerable antibacterial potency against a panel of multi-drug resistant (MDR) bacterial strains. The best MIC values were obtained with compound (2) against S. aureus ATCC 25923 (32 µg/mL) and MRSA1 (16 µg/mL).

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Chemical and Biological Investigations of Justicia gendarussa (Burm. f)

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v10i1.10016 ◽

2012 ◽

Vol 10 (1) ◽

pp. 53-57

Author(s):

Md Rakib Uddin ◽

Suchana Sinha ◽

Md Aslam Hossain ◽

Md Abul Kaisar ◽

Md Khalid Hossain ◽

...

Keyword(s):

Petroleum Ether ◽

Methanol Extract ◽

Soluble Fraction ◽

Diffusion Method ◽

Cytotoxic Activities ◽

Zone Of Inhibition ◽

Disc Diffusion Method ◽

Whole Plant ◽

Ic50 Value ◽

Crude Methanol Extract

In this study, three compounds, stigmasterol (1), lupeol (2), 16-hydroxylupeol (3) were isolated from the petroleum ether soluble fraction of a methanol extract of whole plant of Justica gendarussa (Burm. f). The crude methanol extract as well as its petroleum ether, carbon tetrachloride and chloroform soluble Kupchan fractions were studied for antioxidant, antimicrobial and cytotoxic activities. Among the different fractions tested for antioxidant activity, the chloroform soluble partitionate was the most potent with IC50 value of 18.80 μg/ml as compared to tertbutyl- 1-hydroxytoluene (IC50=17.69 μg/ml). Antimicrobial screening of the different extractives was conducted by the disc diffusion method and all the fractions except chloroform soluble partitionate exhibited mild antimicrobial activity with zone of inhibition ranging from 7-10 mm. In brine shrimp lethality bioassay, the petroleum ether soluble materials demonstrated the highest toxicity with LC50 of 1.27μg /ml. This is the first report of isolation of compounds from J. gendarussa, and its antioxidant, antimicrobial and cytotoxic properties. DOI: http://dx.doi.org/10.3329/dujps.v10i1.10016 DUJPS 2011; 10(1): 53-57

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Membrane Stabilizing and Thrombolytic Activities of Sida rhombifolia L.

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v17i1.22313 ◽

2015 ◽

Vol 17 (1) ◽

pp. 43-45

Author(s):

Mohammd Firoz Khan ◽

Sikder Nahidul Islam Rabbi ◽

Md Aslam Hossain ◽

Mohammad A Rashid

Keyword(s):

Salicylic Acid ◽

Human Erythrocyte ◽

Methanol Extract ◽

Pharmaceutical Journal ◽

Clot Lysis ◽

Hypotonic Solution ◽

Thrombolytic Activity ◽

Whole Plant ◽

Crude Methanol Extract ◽

Sida Rhombifolia

The crude methanol extract of whole plant of Sida rhombifolia and different fractions generated from it were tested for membrane stabilizing and thrombolytic activities. In hypotonic solution- and heat-induced conditions, the chloroform soluble partitionates inhibited haemolysis of human erythrocyte by 22.11±0.14% and 64.71±0.08%, respectively as compared to 71.97±0.51% and 40.12±0.29% demonstrated by the standard acetyl salicylic acid (ASA). Moreover, the pet ether soluble materials revealed highest thrombolytic activity with clot lysis value of 19.51±0.03% as compared to 66.98±0.11% exhibited by the standard streptokinase. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22313 Bangladesh Pharmaceutical Journal 17(1): 43-45, 2014

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Phenolic Acids and Depsides from Some Species of the Erodium Genera

Zeitschrift für Naturforschung C ◽

10.1515/znc-2001-11-1205 ◽

2001 ◽

Vol 56 (11-12) ◽

pp. 943-950 ◽

Cited By ~ 13

Author(s):

Izabela Fecka ◽

Adam Kowalczyk ◽

Wojciech Cisowski

Keyword(s):

Silica Gel ◽

Protocatechuic Acid ◽

Methanol Extract ◽

Polyphenolic Compounds ◽

Methyl Gallate ◽

Chemically Modified ◽

Erodium Cicutarium ◽

Acid Methyl ◽

Whole Plant ◽

Unmodified Silica

Abstract Six natural polyphenolic compounds, brevifolin carboxylic acid, brevifolin, ellagic acid, methyl gallate, gallic acid and protocatechuic acid have been isolated from the methanol extract of the whole plant of Erodium cicutarium (L.) L.’Herit. (Geraniaceae). Structures were determined by conventional methods of analysis and confirmed by MS and NMR spectral analysis. The distribution of these compounds in the other species of the Erodium genera (E. botrys, E. chium, E. ciconium, E. cicutarium, E. glutinosum subsp. dunense, E. gruinum, E. manescavi, E. pelargoniiflorum, E. petraeum ) were examined by HPLC with a RP-18 column, and MGD-TLC methods on unmodified silica gel and silica gel chemically modified with polar and nonpolar groups (HPTLC-Si 60 LiChrospher, HPTLC-NH2, HPTLC-DIOL, HPTLC RP-18W).

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Anticariogenic potential of Potentilla fulgens extract and its chemical constituents

International Journal of Phytomedicine ◽

10.5138/09750185.1939 ◽

2017 ◽

Vol 9 (1) ◽

pp. 83 ◽

Cited By ~ 1

Author(s):

Alka Choudhary ◽

Umesh Bihade ◽

Amit Kumar Mittal ◽

Anupam Chatterjee ◽

Uttam Chand Banerjee ◽

...

Keyword(s):

Biofilm Formation ◽

Antibacterial Agents ◽

Methanol Extract ◽

Chemical Constituents ◽

Lactobacillus Rhamnosus ◽

Bacterial Strains ◽

North East India ◽

North East ◽

Time Kill ◽

Crude Methanol Extract

Streptococci and Lactobacilli are the most common bacteria causing dental caries. Potentilla fuglens, native of north-east India is used by tribal people to cure teeth and gum problems. Based on its ethomedical use, its anticariogenic potential was evaluated. The crude methanol extract was found to have good anticariogenic potential. Epigallocatechingallate (4) was found to the most effective inhibitor against the tested bacterial strains. Epiafzelechin (4β→8) epicatechin (8) also demonstrated comparatively better activity than other dimeric compounds (MIC of 1.56, 3.12 and 3.12 μg/mL against Streptococcus mutans, Lactobacillus acidophilus and Lactobacillus rhamnosus, respectively). Time-kill studies and biofilm formation inhibition assays showed molecule 8 to be comparable to compound 4 in terms of antibacterial action. The results suggest that these compounds and Potentilla extracts could be employed as natural antibacterial agents in oral health care products.

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Chemical and Biological Investigations of Samanea saman (Jacq.) Merr.

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v9i2.7882 ◽

1970 ◽

Vol 9 (2) ◽

pp. 69-73

Author(s):

Faisol Ferdous ◽

Md Khalid Hossain ◽

Mohammad S Rahman ◽

Md Aslam Hossain ◽

Shaila Kabir ◽

...

Keyword(s):

Antimicrobial Activity ◽

Carbon Tetrachloride ◽

Brine Shrimp ◽

Methanol Extract ◽

Preliminary Screening ◽

Brine Shrimp Lethality ◽

Whole Plant ◽

Samanea Saman ◽

First Time ◽

Crude Methanol Extract

Two compounds namely lupeol (1) and epilupeol (2) were isolated from n-hexane soluble fraction of crude methanol extract of the powdered whole plant of Samanea saman Merr. for the first time. The structures of the isolated compounds were established by extensive analyses of their high resolution NMR spectral data as well as co- TLC with authentic samples. In our preliminary screening, the n-hexane, carbon tetrachloride, and dichloromethane soluble fractions of the crude methanolic extract of S. saman were subjected to antimicrobial activity and brine shrimp lethality bioassay. The carbon tetrachloride soluble partitionate of the methanol extract exhibited mild to moderate antimicrobial activity and strong cytotoxicity having LC50 of 0.831μg/ml. Key words: Samanea saman; Leguminosae; lupeol; epilupeol; antimicrobial activity; brine shrimp lethality bioassay. DOI: http://dx.doi.org/10.3329/dujps.v9i2.7882 Dhaka Univ. J. Pharm. Sci. 9(2): 69-73, 2010 (December)

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Antimicrobial Activity of Protein Fraction from Naja ashei Venom against Staphylococcus epidermidis

Molecules ◽

10.3390/molecules25020293 ◽

2020 ◽

Vol 25 (2) ◽

pp. 293 ◽

Cited By ~ 6

Author(s):

Aleksandra Bocian ◽

Ewa Ciszkowicz ◽

Konrad K. Hus ◽

Justyna Buczkowicz ◽

Katarzyna Lecka-Szlachta ◽

...

Keyword(s):

Staphylococcus Epidermidis ◽

Bacterial Infections ◽

Antibacterial Properties ◽

Protein Composition ◽

Exchange Chromatography ◽

Drug Resistant ◽

Bacterial Cells ◽

Bacterial Strains ◽

Snake Venoms ◽

Phospholipases A2

One of the key problems of modern infectious disease medicine is the growing number of drug-resistant and multi-drug-resistant bacterial strains. For this reason, many studies are devoted to the search for highly active antimicrobial substances that could be used in therapy against bacterial infections. As it turns out, snake venoms are a rich source of proteins that exert a strong antibacterial effect, and therefore they have become an interesting research material. We analyzed Naja ashei venom for such antibacterial properties, and we found that a specific composition of proteins can act to eliminate individual bacterial cells, as well as the entire biofilm of Staphylococcus epidermidis. In general, we used ion exchange chromatography (IEX) to obtain 10 protein fractions with different levels of complexity, which were then tested against certified and clinical strains of S. epidermidis. One of the fractions (F2) showed exceptional antimicrobial effects both alone and in combination with antibiotics. The protein composition of the obtained fractions was determined using mass spectrometry techniques, indicating a high proportion of phospholipases A2, three-finger toxins, and L-amino acids oxidases in F2 fraction, which are most likely responsible for the unique properties of this fraction. Moreover, we were able to identify a new group of low abundant proteins containing the Ig-like domain that have not been previously described in snake venoms.

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EVALUATION OF IN VTRO ANTI BACTERIAL ACTIVITY OF THE BREYNIA PATENS (ROXB.) BENTH. & HOOK. F. EXTRACTS

Kongunadu Research Journal ◽

10.26524/krj73 ◽

2015 ◽

Vol 2 (1) ◽

pp. 94-96

Author(s):

Muthuraj K ◽

Abdul Kaffoor H ◽

Nagarajan N

Keyword(s):

Antibacterial Properties ◽

Well Being ◽

Inhibition Zone ◽

Bacterial Activity ◽

Disc Diffusion ◽

Bacterial Strains ◽

Inhibition Zone Diameter ◽

Whole Plant ◽

Novel Drug ◽

Health And Well Being

The plants provide a source of inspiration for novel drug compounds, as plant derived medicines made large contributions to human health and well being. The Present investigation is about the antibacterial activity of Breynia patens (Roxb.) Benth. & Hook.f. against five bacterial strains using the disc diffusion agarmethod. The whole plant extracted with ethanol, chloroform, petroleum ether and Aqueous. The sensitivity order of the Methanol crude extracts was illustrated by the corresponding inhibition zone diameter to be Salmonella paratyphii(20±009mm zone of incubation) and followed by other bacterial strains. The resultsindicated that the plant extract exhibited antibacterial properties, thus justifying scientifically their traditional uses as medicinal plants

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Antibacterial properties of snake venom components

Chemical Papers ◽

10.1007/s11696-019-00939-y ◽

2019 ◽

Vol 74 (2) ◽

pp. 407-419 ◽

Cited By ~ 5

Author(s):

Aleksandra Bocian ◽

Konrad K. Hus

Keyword(s):

Snake Venom ◽

Antibacterial Properties ◽

Drug Resistant ◽

Bacterial Strains ◽

Snake Venoms ◽

Bactericidal Properties ◽

Human Defensins ◽

Promising Source ◽

Proteins And Peptides ◽

Phospholipases A

Abstract An increasing problem in the field of health protection is the emergence of drug-resistant and multi-drug-resistant bacterial strains. They cause a number of infections, including hospital infections, which currently available antibiotics are unable to fight. Therefore, many studies are devoted to the search for new therapeutic agents with bactericidal and bacteriostatic properties. One of the latest concepts is to search for this type of substances among toxins produced by venomous animals. In this approach, however, special attention is paid to snake venom because it contains molecules with antibacterial properties. Thorough investigations have shown that the phospholipases A2 (PLA2) and l-amino acids oxidases (LAAO), as well as fragments of these enzymes, are mainly responsible for the bactericidal properties of snake venoms. Some preliminary research studies also suggest that fragments of three-finger toxins (3FTx) are bactericidal. It has also been proven that some snakes produce antibacterial peptides (AMP) homologous to human defensins and cathelicidins. The presence of these proteins and peptides means that snake venoms continue to be an interesting material for researchers and can be perceived as a promising source of antibacterial agents.

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Antibacterial properties of thalloid liverworts Marchantia polymorpha L., Conocephalum conicum (L.) Dum. and Pellia endiviifolia (Dicks.) Dumort

Journal of the Serbian Chemical Society ◽

10.2298/jsc210728084i ◽

2021 ◽

pp. 84-84

Author(s):

Ivana Ivkovic ◽

Danka Bukvicki ◽

Miroslav Novakovic ◽

Stefan Ivanovic ◽

Olja Stanojevic ◽

...

Keyword(s):

1H Nmr Spectroscopy ◽

Methanol Extract ◽

Antibacterial Properties ◽

Antimicrobial Effect ◽

Marchantia Polymorpha ◽

Gram Negative Bacteria ◽

Macrocyclic Compounds ◽

Bacterial Strains ◽

Conocephalum Conicum ◽

Pellia Endiviifolia

Antimicrobial activity of methanol extracts of three thalloid liverworts, Marchantia polymorpha, Conocephalum conicum and Pellia endiviifolia and bis-bibenzyl marchantin A, the most dominant compound of M. polymorpha methanol extract, have been investigated in this research. 1H NMR spectroscopy revealed that the liverwort?s extract contains terpenes, oils, sugars and bis-bibenzyls in M. polymorpha and P. endiviifolia, while these specific macrocyclic compounds are absent from the C. conicum extract. The antimicrobial potential was tested on eight bacterial strains. The antimicrobial effect of extracts and marchantin A was observed against Gram-positive, while it showed no effect against Gram-negative bacteria in both methods used - well diffusion and broth microdilution.

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