scholarly journals Antimicrobial Susceptibility of Vibrio Species Isolated From Brackish Water Shrimp Culture Environment

2012 ◽  
Vol 36 (2) ◽  
pp. 213-220
Author(s):  
M S Hossain ◽  
M Aktaruzzaman ◽  
A N M Fakhruddin ◽  
M J Uddin ◽  
S H Rahman ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Penaeus Monodon ◽  
Vibrio Species ◽  
Shrimp Farming ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Shrimp Culture ◽  
Antibiotic Resistant ◽  
Culture Environment ◽  
Academy Of Sciences

A comparative study regarding prevalence and antimicrobial susceptibility of Vibrio species in   the black tiger shrimp (Penaeus monodon Fabricius) culture environment as well as market samples   were analyzed. Total Vibrio counts were found to range up to 2.5 × 103 cfu/gm and 60 cfu/gm in   shrimp and Gher water samples, respectively. Results revealed that the total vibrio count were found to be higher in samples taking from the market shops in Dhaka city than Gher shrimp samples and the live shrimps were not contaminated with Vibrio species but dead shrimps were   found contaminated, which were collected from shrimp Gher. In the antimicrobial susceptibility test, highest 28.57% resistance among total Vibrio isolates was observed to penicillin and cephalexin. Among the seven isolates of Vibrio species from Gher water sample one isolate displayed multi-drug resistance (MDR). The main reason of antibiotic resistance could be the indiscriminate application of antibiotics in shrimp farming and release of shrimp pond effluent to estuarine ecosystems or post harvest contamination of shrimps with the antibiotic resistant bacteria   through the environment and human handling. DOI: http://dx.doi.org/10.3329/jbas.v36i2.12964 Journal of Bangladesh Academy of Sciences, Vol. 36, No. 2, 213-220, 2012

Occurrence of antibiotic resistant bacteria in some shrimp farms of Bangladesh

2013 ◽  
Vol 41 (2) ◽  
pp. 197-200 ◽  
Author(s):  
Farzana Ashrafi Neela ◽  
Md Atiquer Rahman ◽  
MST Nasrin Akhter Banu ◽  
M Habibur Rahman ◽  
Hiroyuki Ohta ◽  
...  
Keyword(s):  
Brackish Water ◽  
Water Samples ◽  
Farming System ◽  
Shrimp Farming ◽  
Resistant Bacteria ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Antibiotic Resistant ◽  
Acinetobacter Sp ◽  
Shrimp Farms

Tetracycline (TC) and ampicillin (AMP) resistant bacteria were identified from both fresh and brackish water shrimp farming system, in Bangladesh. Among 78 isolates from freshwater samples, 14.10, 17.95 and 23.8% were found resistant to TC, AMP and TC plus AMP, respectively. On the other hand, isolates from the brackish water samples, the percentages of TC, AMP and TC plus AMP resistant isolates were 10.25, 12.82 and 15.38, respectively. In freshwater samples, the highest percentage of resistant bacteria was Bacillus sp. (38.9), Pseudomonas sp. (22.2), Staphylococcus sp. (16.7), Acinetobacter sp. (11.1), Brevibacillus sp. (5.5) and Enterobacter sp. (5.5). While in brackish water samples dominant resistant bacteria was Bacillus sp. (50) followed by Pseudomonas sp. (16.7), Acinetobacter sp. (16.7), Enterobacter sp. (8.3) and Microvirgula sp. (8.3). DOI: http://dx.doi.org/10.3329/bjb.v41i2.13449 Bangladesh J. Bot. 41(2): 197-200, 2012 (December)

scholarly journals Genome Characterization of the Novel Lytic Genome Sequence of Phage YUEEL01 of the Myoviridae Family

2021 ◽  
Author(s):  
Gurusamy Raman ◽  
Ayyaru Sivasankaran ◽  
SeonJoo Park ◽  
Young-Ho Ahn
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Animal Health ◽  
Gc Content ◽  
Phenotypic Characterization ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Urban Hospital ◽  
Protein Coding ◽  
Antibiotic Resistant

Abstract Antimicrobial resistance is a global concern due to its rapid emergence in the environment and the associated high risk to human and animal health. Municipal wastewater, including urban, hospital, and pharmaceutical effluent, is the main source of antibiotic and antibiotic resistant bacteria (ARB) contamination, and biological processes are commonly used for wastewater treatment. Biologically based strategies seem to be a promising approach to effective integrated ARB control since they can focus on the bases of antibiotic resistance. To develop an effective bacteriophage against multi-drug resistance (MDR) microbes in municipal wastewater, phage YUEEL01 was isolated from a livestock farm, and its genome sequenced. Phenotypic characterization suggested that the phage is a member of the Myoviridae family, and that it encodes 168,266 bp with an overall GC content of 35.4% had 259 putative protein-coding genes, 11 tRNA and six hypothetical genes. A number of putative DNA replication and regulation, DNA packing and structure and host lysis genes were identified. Further, whole genome phylogenetic analysis demonstrated that phage YUEEL01 is closely related to phage slur03. Elucidation of the YUEEL01 phage might be helpful when developing antibacterial tools for controlling MDR bacteria in wastewater treatment systems.

Penaeid shrimp (Penaeus monodon) culture in Bangladesh.

2020 ◽  
Author(s):  
Khandaker Huq ◽  
Shafiq-ur-Rahman
Keyword(s):  
Rural Areas ◽  
Saline Water ◽  
Economic Value ◽  
Penaeus Monodon ◽  
Penaeid Shrimp ◽  
Shrimp Farming ◽  
Grazing Land ◽  
Shrimp Culture ◽  
Saline Water Intrusion ◽  
Employment Generation

Abstract The shrimp (Penaeus monodon), locally known as bagda, is known as white gold for its high economic value in Bangladesh. The shrimp sector of Bangladesh is seen as having become important in economic terms, contributing significantly to foreign exchange earnings and employment generation in rural areas. P. monodon culture is practiced in the Bagerhat, Chokoria, Cox's Bazar, Khulna, Moheshkhali, Shatkhira and Teknaf regions and recently has been introduced in some coastal areas of Noakhali district. Three culture systems are found in Bangladesh, i.e. traditional, improved traditional and semi-intensive with production rates of 150-200, 250-400 and 2,000-2500 kg ha-1, respectively. Seed is collected from natural sources and is produced in hatcheries. There are 44 penaeid shrimp hatcheries in Bangladesh. The present shrimp fry production is 3.05 billion. More than 200,000 people are engaged in shrimp fry collection and marketing and more than 350,000 people are engaged in shrimp farming. Ancillary industries like shrimp hatcheries, feed industries, ice factories, processing factories, net industries and shrimp culture apparatus industries provide direct employment for 25,000-30,000 people. Adverse environmental impacts reported are reduction of grazing land and saline water intrusion due to mismanagement but these could be overcome by introducing environmentally friendly shrimp culture. As the climate of Bangladesh is favourable for P. monodon culture, it is necessary to give more attention to improve it as it will open a new horizon in the economy of the country.

scholarly journals Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

2019 ◽  
Author(s):  
Cian Smyth ◽  
Aidan O’Flaherty ◽  
Fiona Walsh ◽  
Thi Thuy Do
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Control Point ◽  
Treated Wastewater ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Faecal Coliforms ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Extended Spectrum

AbstractWastewater treatment plants (WWTPs) provide optimal conditions for the maintenance and spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this work we describe the occurrence of antibiotic resistant faecal coliforms and their mechanisms of antibiotic resistance in the effluent of two urban WWTPs in Ireland. Effluent samples were collected from two WWTPs in Spring and Autumn of 2015 and 2016. The bacterial susceptibility patterns to 13 antibiotics were determined. The phenotypic tests were carried out to identify AmpC or extended-spectrum β-lactamase (ESBL) producers. The presence of ESBL genes were detected by PCR. Plasmids carrying ESBL genes were transformed into Escherichia coli DH5α recipient and underwent plasmid replicon typing to identify incompatibility groups. More than 90% of isolated faecal coliforms were resistant to amoxicillin and ampicillin, followed by tetracycline (up to 39.82%), ciprofloxacin (up to 31.42%) and trimethoprim (up to 37.61%). Faecal coliforms resistant to colistin and imipenem were detected in all effluent samples. Up to 53.98% of isolated faecal coliforms expressed a multi-drug resistance (MRD) phenotype. AmpC production was confirmed in 5.22% of isolates. The ESBL genes were confirmed for 11.84% of isolates (9.2% of isolates carried blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15). Plasmids extracted from 52 ESBL isolates were successfully transformed into recipient E. coli. The detected plasmid incompatibility groups included the IncF group, IncI1, IncHI1/2 and IncA/C. These results provide evidence that treated wastewater is polluted with ARB and MDR faecal coliforms and are sources of ESBL-producing, carbapenem and colistin resistant Enterobacteriaceae.ImportanceAntibiotic resistant bacteria (ARB) are an emerging environmental concern with a potential impact on human health. The results provide the evidence that treated wastewater is polluted with antibiotic resistant bacteria containing mobile resistance mechanisms of importance to clinical treatment of pathogens and multi-drug resistant (MDR) faecal coliforms. They are sources of relatively high proportions of ESBL-producing Enterobacteriaceae, and include carbapenem and colistin resistant Enterobacteriaceae. The significance of this study is the identification of the role of WWTPs as a potential control point to reduce or stop the movement of ESBL, MDR and colistin resistant bacteria into the environment from further upstream sources, such as human or animal waste.

scholarly journals Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

2019 ◽  
Author(s):  
Cian Smyth ◽  
Aidan O’Flaherty ◽  
Fiona Walsh ◽  
Thi Thuy Do
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Control Point ◽  
Treated Wastewater ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Faecal Coliforms ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Extended Spectrum

AbstractWastewater treatment plants (WWTPs) provide optimal conditions for the maintenance and spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this work we describe the occurrence of antibiotic resistant faecal coliforms and their mechanisms of antibiotic resistance in the effluent of two urban WWTPs in Ireland. Effluent samples were collected from two WWTPs in Spring and Autumn of 2015 and 2016. The bacterial susceptibility patterns to 13 antibiotics were determined. The phenotypic tests were carried out to identify AmpC or extended-spectrum β-lactamase (ESBL) producers. The presence of ESBL genes were detected by PCR. Plasmids carrying ESBL genes were transformed into Escherichia coli DH5α recipient and underwent plasmid replicon typing to identify incompatibility groups. More than 90% of isolated faecal coliforms were resistant to amoxicillin and ampicillin, followed by tetracycline (up to 39.82%), ciprofloxacin (up to 31.42%) and trimethoprim (up to 37.61%). Faecal coliforms resistant to colistin and imipenem were detected in all effluent samples. Up to 53.98% of isolated faecal coliforms expressed a multi-drug resistance (MRD) phenotype. AmpC production was confirmed in 5.22% of isolates. The ESBL genes were confirmed for 11.84% of isolates (9.2% of isolates carried blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15). Plasmids extracted from 52 ESBL isolates were successfully transformed into recipient E. coli. The detected plasmid incompatibility groups included the IncF group, IncI1, IncHI1/2 and IncA/C. These results provide evidence that treated wastewater is polluted with ARB and MDR faecal coliforms and are sources of ESBL-producing, carbapenem and colistin resistant Enterobacteriaceae.ImportanceAntibiotic resistant bacteria (ARB) are an emerging environmental concern with a potential impact on human health. The results provide the evidence that treated wastewater is polluted with antibiotic resistant bacteria containing mobile resistance mechanisms of importance to clinical treatment of pathogens and multi-drug resistant (MDR) faecal coliforms. They are sources of relatively high proportions of ESBL-producing Enterobacteriaceae, and include carbapenem and colistin resistant Enterobacteriaceae. The significance of this study is the identification of the role of WWTPs as a potential control point to reduce or stop the movement of ESBL, MDR and colistin resistant bacteria into the environment from further upstream sources, such as human or animal waste.

IMPACT OF WHITE SPOT SYNDROME VIRUS DISEASE ON GILLS IN SHRIMP, PENAEUS MONODON

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
C.A. JAWALE ◽  
B.J. UGALE
Keyword(s):  
White Spot Syndrome Virus ◽  
Virus Disease ◽  
Penaeus Monodon ◽  
Penaeid Shrimp ◽  
Shrimp Farming ◽  
Protozoan Parasites ◽  
Shrimp Culture ◽  
Histological Changes ◽  
Histological Techniques ◽  
Gill Cells

Protozoan parasites cause problems in Penaeid shrimp culture and major impact on the shrimp farming industry.White spot syndrome virus (WSSV) is a major shrimp pathogen that also infects Penaeus monodon species was selected. Histological changes in the gill cells were studied. Histological techniques using paraffin embeded tissues as well as frozen tissues were used for identification of WSSV infection. Histological manifestation such as gill cells could be detected. The gill cells were marked and characteristic of WSSV infections were observed. The present investigation was focused on the virus infecting shrimp. The microscopic examinations of the histological preparation are presently used to detect WSSV zoothamnium.

scholarly journals Presence of Antibiotic Residues and Antibiotic Resistant Bacteria in Cattle Manure Intended for Fertilization of Agricultural Fields: A One Health Perspective

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 410
Author(s):  
Judith Huygens ◽  
Els Daeseleire ◽  
Jacques Mahillon ◽  
Daan Van Elst ◽  
Johan Decrop ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Farmyard Manure ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Antibiotic Residues ◽  
Agricultural Field ◽  
Antibiotic Resistant Bacteria ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
E Coli

Antibiotic resistant bacteria and antibiotic residues can enter the environment when using animal manure as fertilizer. Twenty-five mixed beef cattle farmyard manure samples and 9 mixed fattening calf slurry samples from different farms across Belgium were investigated for the presence of 69 antibiotic residues, antibiotic resistant Escherichia coli and Salmonella spp. Doxycycline, oxytetracycline, ciprofloxacin, enrofloxacin, flumequine and lincomycin were detected in all fattening calf slurry samples with mean concentrations of 2776, 4078, 48, 31, 536 and 36 µg/kg manure, respectively. Sulfadiazine was detected at a mean concentration of 10,895 µg/kg. Further, antibiotic residues were found in only 4 of the 25 beef cattle farmyard manure samples. Oxytetracycline was detected twice below 500 µg/kg. Paromomycin, ciprofloxacin and enrofloxacin were detected in a concentration below 100 µg/kg. Of E. coli isolates, 88% and 23% from fattening calf slurry and beef cattle farmyard manure, respectively, were resistant to at least one of the antibiotics tested. Multi-drug resistance was observed at a maximum of 10 and 7 antibiotics, respectively. The occurrence of antibiotic resistant E. coli and antibiotic residues is shown to be higher in fattening calf slurry than in beef cattle farmyard manure used for agricultural field fertilization.

scholarly journals Synthesis and Characterization of Carbapenem Based Nanohybrids as Antimicrobial Agents for Multidrug Resistant Bacteria

2019 ◽  
Vol 56 (2) ◽  
pp. 388-391
Author(s):  
Letitia Doina Duceac ◽  
Geta Mitrea ◽  
Elena Ariela Banu ◽  
Madalina Irina Ciuhodaru ◽  
Irina Mihaela Ciomaga ◽  
...  
Keyword(s):  
Antibacterial Therapy ◽  
Antimicrobial Agents ◽  
Chitosan Nanoparticles ◽  
Multidrug Resistant ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Antibiotic Resistant ◽  
Resistant Strains

Carbapenem antibiotics resistance is a medical threat in antibacterial therapy as the pathogen resistant strains easily evolve a multi-drug resistance action to other incurable agents. The protective transport of current antibiotic molecules using nano-carriers initiates a huge approach in the antibacterial therapy, allowing the nanohybrids to defeat all these health threat pathogen agents. Chitosan is a linear cationic polysaccharide being often used in medical area as a biocompatible encapsulating agent in antibiotic delivery nanosystems. This work refers to encapsulation of imipenem into biodegradable chitosan nanoparticles in order to destroy antibiotic-resistant bacteria and limit the microbial adhesion and multiplication. Nanoparticles were prepared by ion gelation method using tripolyphosphate as cross linking agent. The obtained hybrid nanocapsules were then characterized and evaluated as a potential nano-device to beat antimicrobial resistance.

scholarly journals Occurrence of Antibiotic Residues and Antibiotic-Resistant Bacteria in Nile Tilapia Sold in Some Markets in Accra, Ghana: Public Health Implication

2018 ◽  
Vol 7 (6) ◽  
pp. 129 ◽  
Author(s):  
Eric S. Donkor ◽  
Isaac Anim-Baidoo ◽  
Evans Fei ◽  
Collins Amponsah ◽  
Michael Olu-Taiwo ◽  
...  
Keyword(s):  
Public Health ◽  
Nile Tilapia ◽  
Capital City ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Antibiotic Residues ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Public Health Implication ◽  
Fish Samples

In Ghana there are concerns that antibiotics may be used inappropriately to boost fish production, though no study has investigated this problem. To provide preliminary insights into public health aspects of the problem, we investigated the occurrence of antibiotic residues and antibiotic-resistant bacteria in Nile tilapia (Oreochromis niloticus), a fish commonly cultivated and consumed in Ghana. Two hundred Nile Tilapia fish were randomly sampled from four major markets in Accra, the capital city of Ghana. One hundred samples were screened for antibiotic residues using a microbial inhibition plate test that detects sixteen different antibiotics commonly used in animal husbandry and aquaculture. The other 100 samples were cultured for bacteria using direct culture methods, and the isolates were tested against seven antibiotics by the Kirby Bauer method. The overall prevalence of antibiotic residues in the fish samples was 7%. Bacteria that were isolated from the fish samples were Shigella sonnei (10%), Enterobacter cloacae (7%), Escherichia coli (6%), Salmonella Typhi (3%) and Klebsiella pneumoniae and Proteus mirabilis (2%). All bacteria isolated were susceptible to gentamicin and ciprofloxacin but resistant to ampicillin. Multi-drug resistance (ie resistance to three or more different classes of antibiotics) occurred in 86.7% of the isolates. Nile Tilapia sold in Accra is a source of multi-drug resistant bacteria. Consumption of the fish can also lead to significant exposure to antibiotic residues.

scholarly journals Occurrence and Antimicrobial Susceptibility Patterns of Staphylococcus ‎aureus and Salmonella species in Fresh Milk and Milk Products Sold in Zaria ‎and Environs, Kaduna State, Nigeria

2021 ◽  
Vol 18 (2) ◽  
pp. 1-8
Author(s):  
D. O. Esonu ◽  
S. Ismail ◽  
A. Ajala ◽  
S. M. Yusuf ◽  
R. G. Otolorin
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Public Awareness ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Salmonella Spp ◽  
Milk Products ◽  
Pasteurized Milk ◽  
Antimicrobial Susceptibility Pattern ◽  
Fresh Milk

Staphylococcal infections and salmonellosis are important food-borne diseases affecting both humans and animals, and the spread of antibiotic resistant bacteria to humans through the consumption of animal food products remains a challenge. To determine the occurrence and antimicrobial susceptibility pattern of Staphylococcus aureus and Salmonella in fresh milk and milk products sold in Zaria and environs, Kaduna State, Nigeria, ninety samples were purchased comprising of 49 pasteurized milk, 20 ghee (Clarified butter) and 21 fresh milk samples in Zaria. The Samples were pre-enriched before plating on a suitable selective media. Biochemical and Sugar fermentation tests were used to detect S. aureus and Salmonella isolates; also, antimicrobial susceptibility test was carried out on positive isolates. The overall prevalence of S. aureus and Salmonella spp. in fresh milk and milk products (pasteurized milk and ghee) were 31.1% (28/90) and 6.7% (6/90), respectively. Higher prevalence of S. aureus was found in fresh milk (38.1%) than in ghee (35%) and pasteurized milk (26.5%). Also, a higher prevalence of Salmonella organism was found in fresh milk (9.5%) than in pasteurized milk (6.1%) and ghee (5%). Positive iso­lates of S. aureus showed the highest percentage of antibiotic resistance to oxacillin (100%), then tetracycline (64%) and vancomycin (57.1%), while positive iso­lates of Salmonella showed oxacillin (100%) and vancomycin (100%), followed by tetracycline (33.3%). Most of the isolates displayed resistance to more than three (3) different classes of antimicrobials, indicating multi-drug resistance. Therefore, there is a need for education and public awareness regarding hygienic processing, handling and storage of fresh milk and milk products; thus, the abuse and indiscriminate use of antibiotics in animals should be discouraged.

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