scholarly journals Pathogenicity of the bacterial isolate Aeromonas hydrophila to catfishes, carps and perch

2012 ◽  
Vol 10 (1) ◽  
pp. 157-161 ◽  
Author(s):  
M J A Sarkar ◽  
M M Rashid
Keyword(s):  
Aeromonas Hydrophila ◽  
Bacterial Isolate ◽  
Labeo Rohita ◽  
Bacterial Load ◽  
Catla Catla ◽  
Anabas Testudineus ◽  
Highly Virulent ◽  
Average Body ◽  
Different Doses ◽  
Average Body Weight

Pathogenicity of a bacterial isolate Aeromonas hydrophila recovered from naturally diseased shing fish was investigated against catfishes (Heteropneustes fossilis and Clarias batrachus), carps (Labeo rohita, Catla catla and  Cirrhinus cirrhosus) and perch (Anabas testudineus) of average body weight of 20.4 g for H. fossilis, 25.6 g for C.  Batrachus, 35.2 g for L. rohita, 25.7 g for C. catla, 30.5 g for C. cirrhosus and 20.3 g for A. testudineus. Two different  doses viz. 6.7 × 106 and 6.7 × 105 CFU/fish were injected intramuscularly. Pathogenicity of A. hydrophila was confirmed at water temperature of 30°C by mortality of 60% to 100% of all the tested fishes within 2-11 days. Injected  A. hydrophila was re-isolated from liver, kidney and intestine of all the tested fishes. The highest bacterial loads in catfishes were found to be 5.5 × 108 CFU/g in the liver of H. fossilis and 5.6 × 107 CFU/g in the intestine of C.  batrachus. The lowest bacterial loads were found to be 2.2 × 102 CFU/g in the kidney of H. fossilis and 2.4 × 103 CFU/g in the liver of C. batrachus. The highest bacterial loads in carps were found to be 4.9 × 109 CFU/g in the liver  of C. catla, 7.7 × 108 CFU/g in the intestine of L. rohita and 5.8 × 108 CFU/g in the intestine of C. cirrhosus. The   lowest bacterial loads were found to be 2.7 × 104 CFU/g in the kidney of C. catla, 3.0 ×104 CFU/g in the kidney of L   rohita and 5.6 × 103 CFU/g in the kidney of C. cirrhosus. The highest and lowest bacterial load in perch was found to  be 6.4 × 10' CFU/g and 1.6 × 102 CFU/g in the intestine and kidney of A. testudineus respectively. In all the cases of  intramuscular injection, external pathology was found. Reddish anal region and fm bases were observed. It was   understood that the isolate was a highly virulent pathogen for the challenged fishes.   DOI: http://dx.doi.org/10.3329/jbau.v10i1.12108   J. Bangladesh Agril. Univ. 10(1): 157–161, 2012  

scholarly journals Experimental Infection of Indigenous Climbing Perch Anabas testudineus with Aeromonas hydrophila Bacteria

2013 ◽  
Vol 22 (1-2) ◽  
pp. 105-114 ◽  
Author(s):  
M Farhad Hossain ◽  
M Mamnur Rahman ◽  
MA Sayed
Keyword(s):  
Body Weight ◽  
Water Temperature ◽  
Aeromonas Hydrophila ◽  
Bacterial Load ◽  
Infected Fish ◽  
Pathogenicity Test ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Average Body ◽  
Average Body Weight

The present study was conducted to know the pathogenicity and LD50 of Aeromonas hydrophila isolated from diseased climbing perch Anabas testudineus against apparently healthy homologous fish and the distribution of the bacteria in the organs of the experimentally infected fish. A total of 10 fish of average body weight of 18 g were used. For pathogenicity test, two different doses viz. 9.2 × 107 and 9.2 × 106 CFU/fish were injected intramuscularly. Pathogenicity of A. hydrophila was confirmed at water temperature of 28.53oC by the mortality of 40% to 100% of all tested fish within 4 to 9 days. The highest bacterial load was found to be 9.4 × 108 CFU/g in the intestine and the lowest bacterial load was found to be 2.8 × 103 CFU/g in the kidney of the tested fish. Four different serial concentrations, vide 9.2 × 107, 9.2 × 106, 9.2 × 105 and 9.2 × 104 CFU/fish of the bacteria were injected in each of four different groups of 10 fish. The calculated LD50 value at 27.3oC water temperature was 2 × 107 CFU/fish of 18 g of average body weight. In all the cases of intramuscular injection, external pathology was found. Reddish anal region and fin bases were observed. Injected A. hydrophila was re-isolated from liver, kidney and intestine of the challenged fish. It was understood that the isolate was a high virulent pathogen for A. testudineus.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16472 Progress. Agric. 22(1 & 2): 105-114, 2011

scholarly journals Isolation, molecular identification and antibiotic susceptibility profile of Aeromonas hydrophila from cultured indigenous Koi (Anabus testudineus) of Bangladesh

2016 ◽  
Vol 2 (2) ◽  
pp. 332-340
Author(s):  
Shuvho Chakra Borty ◽  
Fabaya Rahman ◽  
AKM Ali Reza ◽  
Mst Sharifa Khatun ◽  
Md Luthful Kabir ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Bacterial Load ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Isolation And Identification ◽  
Anabas Testudineus ◽  
Physiological And Biochemical Characteristics ◽  
Wide Range ◽  
Pcr Products ◽  
Antibiotic Sensitivity Test

Fish play a crucial role in the Bangladeshi diet, providing more than 60% of animal source food, representing a crucial source of micro-nutrients and possessing an extremely strong cultural attachment. In this study isolation and identification of Aeromionas hydrophila was done by studying cultural properties, Gram’s staining and biochemical properties of isolates of diseased indigenous Koi fish (Anabas testudineus) of different upazillas of Mymensingh district. Antibiogram profile of the isolated bacteria was studied by using wide range of commercially available antibiotics. Quantitative study of bacteria isolated from diseased indigenous Koi fish showed variation of number in different organ. Total bacterial load was found to be 1.90 × 105, 1.19 × 105, 3.21 × 105, 2.18 × 106 and 3.14 × 105 cfu/g in lesions; 2.52 × 107, 2.34 × 108, 5.41 × 108, 2.54 × 109 and 5.21 × 109 cfu/g in liver; 2.54 × 108, 2.41 × 108, 1.90 × 107, 3.65 × 107 and 3.45 × 108 cfu/g in spleen; 3.51 × 107, 5.28 × 107, 3.14 × 106, 1.85 × 107 and 4.52× 107 cfu/g in kidney in diseased Koi of Mymensingh sadar, Muktagacha, Tarakanda, Gouripur and Fulpur upazillas, respectively under Mymensingh districts. Aeromonas hydrophila was initially identified by their specific morphological, physiological and biochemical characteristics. Then molecular detection of A. hydrophila was done by PCR. PCR products of desired 760 bp were obtained for A. hydrophila. The results of the antibiotic sensitivity test is exhibited that most of the bacterial samples were sensitive against ciprofloxacin (92%) and levofloxacin (84%), intermediate resistant against gentamicin (40%) and resistant against novobiocin (84%), ampicillin (100%) and penicillin (92%).Asian J. Med. Biol. Res. June 2016, 2(2): 332-340

THE EFFECT OF (Morinda citrifolia L.) MEAL IN DIET ON PERFORMANCE OF BROILER

2012 ◽  
Vol 2 (1) ◽  
pp. 21-27
Author(s):  
Yosi Fenita
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Morinda Citrifolia ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Randomized Design ◽  
Different Levels ◽  
Effect Of Feeding ◽  
Average Body ◽  
Average Body Weight

The objective of the research was to evaluate to effect of feeding mengkudu on performances of broilers. The research design used was completely randomized design. One hundred broilers were distributed into five treatments. The treatments were different levels of mengkudu meal (0, 0.75%, 1.5%, 2.25 % and 3%). The observed measured were feed consumption, average body weight (gain) and feed conversion. Results showed that feeding mengkudu (Morinda Citrifolia L.)  no effect significant (P>0.05) on feed consumption, average body weight and feed conversion.  In conclusion, feeding mengkudu meal up to 3% (in diet) does not negatively affect feed consumption, average body weight, and feed conversion.

scholarly journals The Effects of Feed Color on Broiler Performance between Day 1 and 21

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1511
Author(s):  
Joseph P. Gulizia ◽  
Kevin M. Downs
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Treatment Effects ◽  
Body Weight Gain ◽  
Experimental Period ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Broiler Performance ◽  
Average Body ◽  
Average Body Weight

Two trials were conducted to determine feed color effects on broiler performance. A completely randomized design was used. Trial 1 included four treatments: control (complete broiler starter diet), red, green, and blue; and Trial 2 included four treatments: control, orange, yellow, and purple. Each trial had 4 treatments with 4 replicates (60 birds/treatment) fed to 240 male Cobb 500 broilers during a 21 d grow out. Data were analyzed using the GLM procedure. In Trial 1, there were no treatment effects on average body weight, body weight gain, and feed consumption (p > 0.05). Adjusted feed conversion for control (1.23) was less than red (1.27; p = 0.001) and green (1.26; p = 0.009), with blue (1.25; p = 0.056) tending to be different during the experimental period. In Trial 2, there were no treatment effects on average body weight, feed consumption, and adjusted feed conversion during this study (p > 0.05). Body weight gain between d 1 to 14 for purple (490.78 g/bird) was more than orange (467 g/bird; p = 0.013) and yellow (461 g/bird; p= 0.004), with control (474 g/bird; p = 0.052) tending to be different. Results indicate that these feed colors had some, albeit limited, influence on broiler performance parameters.

Effect of dietary supplemented andrographolide on growth, non-specific immune parameters and resistance against Aeromonas hydrophila in Labeo rohita (Hamilton)

2013 ◽  
Vol 35 (5) ◽  
pp. 1433-1441 ◽  
Author(s):  
Kusunur Ahamed Basha ◽  
Ram Prakash Raman ◽  
Kurcheti Pani Prasad ◽  
Kundan Kumar ◽  
Ezhil Nilavan ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Labeo Rohita ◽  
Immune Parameters

scholarly journals Cross-talk among flesh-eating Aeromonas hydrophila strains in mixed infection leading to necrotizing fasciitis

2016 ◽  
Vol 113 (3) ◽  
pp. 722-727 ◽  
Author(s):  
Duraisamy Ponnusamy ◽  
Elena V. Kozlova ◽  
Jian Sha ◽  
Tatiana E. Erova ◽  
Sasha R. Azar ◽  
...  
Keyword(s):  
Necrotizing Fasciitis ◽  
Mixed Infection ◽  
Aeromonas Hydrophila ◽  
Case Fatality ◽  
Host Immune System ◽  
Complex Interactions ◽  
Solid Media ◽  
Multiple Strains ◽  
Highly Virulent

Necrotizing fasciitis (NF) caused by flesh-eating bacteria is associated with high case fatality. In an earlier study, we reported infection of an immunocompetent individual with multiple strains of Aeromonas hydrophila (NF1–NF4), the latter three constituted a clonal group whereas NF1 was phylogenetically distinct. To understand the complex interactions of these strains in NF pathophysiology, a mouse model was used, whereby either single or mixed A. hydrophila strains were injected intramuscularly. NF2, which harbors exotoxin A (exoA) gene, was highly virulent when injected alone, but its virulence was attenuated in the presence of NF1 (exoA-minus). NF1 alone, although not lethal to animals, became highly virulent when combined with NF2, its virulence augmented by cis-exoA expression when injected alone in mice. Based on metagenomics and microbiological analyses, it was found that, in mixed infection, NF1 selectively disseminated to mouse peripheral organs, whereas the other strains (NF2, NF3, and NF4) were confined to the injection site and eventually cleared. In vitro studies showed NF2 to be more effectively phagocytized and killed by macrophages than NF1. NF1 inhibited growth of NF2 on solid media, but ExoA of NF2 augmented virulence of NF1 and the presence of NF1 facilitated clearance of NF2 from animals either by enhanced priming of host immune system or direct killing via a contact-dependent mechanism.

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