scholarly journals Bacteriological Profile of Diabetic Foot Infection and Its Effect on Limb Salvation

2020 ◽  
Vol 24 (1) ◽  
pp. 21-25
Author(s):  
Mahbub Murshed
Keyword(s):  
Staphylococcus Aureus ◽  
Data Collection ◽  
Diabetic Foot ◽  
Treatment Modalities ◽  
Gram Positive ◽  
Foot Ulceration ◽  
Gram Negative ◽  
Bacteriological Profile ◽  
Data Collection Sheet ◽  
Gram Positive Cocci

Background: Foot ulceration has been reported as the leading cause of hospital admission and amputation in individuals with diabetes. Diabetes-related foot ulcers require multidisciplinary management and care, including debridement, offloading, dressings, management of infection, modified footwear and management of extrinsic factors. Objective: To identify the bacterial pathogens responsible for diabetic foot infections and its effect on limb salvation. Methods: This prospective observational and cross sectional study was conducted among 100 diabetic foot ulceration patients in Mainamati Medical College Hospital, Cumilla between the period of January, 2017 to December, 2018. The patients with diabetic foot ulcer Wagner Grade (2-5) irrespective of age and sex were included in the study. They were thoroughly examined and swab from ulcer base was collected by sterile swab stick and container supplied by the microbiology department. A pre-structured, peer reviewed, interview and observation based data collection sheet was prepared that was used as a research tool. Data regarding clinical, hematological and bacteriological profile were recorded by data collection sheet. Data were entered, managed and analyzed. Results: Diabetic foot was very common in the elderly age group (51-60yrs) 38% and male predominant (78%). Staphylococcus aureus (35%) is the commonest pathogens affecting as the gram positive cocci whereas pseudomonas (23%) is the commonest among the gram negative bacilli. Chloramphenicol, Amikacin, Vancomycin were the most sensitive drugs against gram positive cocci whereas, Cefuroxin, Co-trimoxazole and Gentamycin found the most sensitive drugs against gram negative bacilli. Incision, drainage and dressing (31%) found the commonest treatment modality. The other treatment modalities were debridement, dressing and reconstruction conservative dressing, above and below knee amputation, toe disarticulation etc. Conclusion: Diabetic foot is usually affected by Staphylococcus aureus and streptococcus among the gram positive cocci and pseudomonas whereas Chlamydia and E. coli among the gram negative bacilli. For the limb salvage incision & drainage as well as debridement, excision and reconstruction are the mostly chosen procedure. Journal of Surgical Sciences (2020) Vol. 24 (1) : 21-25

Microbiology of Ventilator–Associated Pneumonia Compared With That of Hospital-Acquired Pneumonia

2007 ◽  
Vol 28 (7) ◽  
pp. 825-831 ◽  
Author(s):  
David J. Weber ◽  
William A. Rutala ◽  
Emily E. Sickbert-Bennett ◽  
Gregory P. Samsa ◽  
Vickie Brown ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Empirical Therapy ◽  
Ventilator Associated Pneumonia ◽  
Gram Positive ◽  
Similar Frequency ◽  
Gram Negative ◽  
Hospital Acquired Pneumonia ◽  
Hospital Acquired ◽  
Gram Positive Cocci ◽  
Ventilated Patients

Objective.Nosocomial pneumonia is the leading cause of mortality attributed to nosocomial infection. Appropriate empirical therapy has been associated with improved survival, but data are limited regarding the etiologic agents of hospital-acquired pneumonia in non-ventilated patients (HAP). This evaluation assessed whether the currently recommended empirical therapy is appropriate for both ventilator-associated pneumonia (VAP) and HAP by evaluating the infecting flora.Design.Prospectively collected hospitalwide surveillance data was obtained by infection control professionals using standard Centers for Disease Control and Prevention definitions.Setting.A tertiary care academic hospital.Patients.All patients admitted from 2000 through 2003.Results.A total of 588 episodes of pneumonia were reported in 556 patients: 327 episodes of VAP in 309 patients, and 261 episodes of HAP in 247 Patients. The infecting flora in ventilated patients included gram-positive cocci (32.0% [oxacillin-susceptible Staphylococcus aureus {OSSA}, 9.25%; oxacillin-resistant Staphylococcus aureus {ORSA}, 17.75%]), gram-negative bacilli (59.0% {Pseudomonas aeruginosa, 17.50%; Stenotrophomonas maltophilia, 6.75%; Acinetobacter species, 7.75%), and miscellaneous pathogens (9.0%). The infecting flora in nonventilated patients included gram-positive cocci (42.59% [OSSA, 13.33%; ORSA, 20.37%]), gram-negative bacilli (39.63% [P. aeruginosa, 9.26%; S. maltophilia, 1.11%; Acinetobacter species, 3.33%), and miscellaneous pathogens (17.78%).Conclusions.Our data demonstrated that patients with HAP, compared with those with VAP, had a similar frequency of infection with ORSA but less commonly had infections due to P. aeruginosa, Acinetobacter species, and S. maltophilia. However, the overall frequency of infection with these pathogens was sufficiently high to warrant the use of empirical therapy likely to be active against them. Our data supports using the currently recommended empirical therapy for both HAP and VAP.

scholarly journals Microbiological Profile of Uropathogens and their Antibiotic Susceptibility Pattern in Pediatric Population from A Tertiary Care Hospital in Amritsar, Punjab

2021 ◽  
Vol 10 (11) ◽  
pp. 306-312
Author(s):  
Manpreet Kaur Bhatha Loveena Oberoi ◽  
Sapna Soneja Anuradha Malhotra ◽  
Kamaldeep Singh
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Pediatric Population ◽  
Age Group ◽  
Susceptibility Pattern ◽  
Gram Positive ◽  
Antibiotic Susceptibility Pattern ◽  
Gram Negative ◽  
Gram Negative Organisms ◽  
Gram Positive Cocci

Urinary tract infection(UTI) is one of the most commonest bacterial infection encountered in the pediatric age group. Early diagnosis is of utmost importance to preserve renal function and reduce long term complications such as renal scarring. The objective of this study was to determine the prevalence of UTI in pediatric population, identify the common uropathogens and study the antibiotic susceptibility pattern of bacterial isolates. Materials and Methods: A retrospective study was carried out in Department of Microbiology, GMC Amritsar for a period of 6 months from 1 Jan 2021 till 30 June 2021 amongst the pediatric patients (1 month-12 years age group) with clinically suspected UTI including both indoor and outdoor patients. Clean catch midstream urine samples received in the laboratory were processed as per the standard microbiological guidelines. Antibiotic susceptibility testing was done using Kirby-Bauer disc diffusion method as per CLSI guidelines. Results: A total of 549 samples were received and processed during study period from which 164(29.87%) were culture positive. Culture positivity was more prevalent in females (61.58%) than males (38.41%). Gram negative organisms were predominant (74.4%) followed by gram positive cocci (22.56%) and Candida (3.05%). Among gram negative organisms Escherichia coli was most predominant followed by Klebsiella spp., Pseudomonas spp., Acinetobacter spp., Proteus spp. and Citrobacter spp. Among gram positive cocci Staphylococcus aureus was most predominant followed by Coagulase negative Staphylococci and Enterococcus spp. Majority of gram negative bacilli were resistant to fluroquinolones, 3rd generation cephalosporins and co-trimoxazole while nitrofurantoin, piperacillin-tazobactam and gentamicin were most sensitive drugs. Among Staphylococcus aureus, methicillin resistance was seen in 17% isolates. High sensitivity was seen to linezolid and vancomycin in gram positive cocci. Conclusion: This study highlights increased prevalence of MDR uropathogens in pediatric population which indicates that antibiotic selection should be based on knowledge of local prevalence of bacterial organisms and their antibiotic sensitivities with rational use of antibiotics.

scholarly journals Bacteriological Profile of Neonatal Sepsis and Antibiogram of the Isolates

2017 ◽  
Vol 37 (1) ◽  
pp. 5-9
Author(s):  
Roshan Parajuli ◽  
Narayan Dutt Pant ◽  
Raju Bhandari ◽  
Anil Giri ◽  
Suman Rai ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Neonatal Sepsis ◽  
Diffusion Method ◽  
Preliminary Treatment ◽  
Biochemical Tests ◽  
Gram Positive ◽  
Cross Sectional ◽  
Gram Negative ◽  
Bacteriological Profile ◽  
Gram Positive Cocci

Introduction: Neonatal sepsis is a serious problem in developing countries like Nepal. The main objectives of this study were to determine the bacteriological profile of neonatal sepsis, to determine the antimicrobial susceptibility patterns of the causative agents and to evaluate the association between the neonatal sepsis and the different characteristics of the neonates.Methods: A hospital based cross-sectional study was conducted among a total of 450 neonates suspected of suffering from sepsis. Blood culture was performed using statdard microbiological techniques. The colonies grown were identified on the basis of colony morphology, Gram’s stain and biochemical tests. The antimicrobial susceptiblility testing was performed by Kirby Bauer disc diffusion method. Results: Out of total 450 blood samples, 92 (20.4%) were culture positive. Of which,  16 (17.4%) samples contained  gram negative bacteria and 76 (82.6%) samples contained gram positive cocci. The most common bacterial pathogens isolated were Staphylococcus epidermidis (67.4%) followed by Escherichia coli (13%). All gram positive cocci were susceptible to vancomycin, while all gram negative bacilli were sensitive to amikacin. There was statistically significant relationship between neonatal sepsis and gestation age of neonates.Conclusions: Neonatal sepsis is still present as a serious problem in Nepal. Staphylococcus epidermidis was the most common cause of the neonatal sepsis. Prematurely delivered neonates should be given more care, as they are more prone to suffering from neonatal sepsis. Vancomycin and amikacin can be used as the drugs of choice for preliminary treatment of neonatal sepsis in our settings.

Common Pathogens and Antibiotic Sensitivity Profiles of Infected Diabetic Foot Ulcers in Saudi Arabia

2018 ◽  
Vol 17 (3) ◽  
pp. 161-168 ◽  
Author(s):  
Mousab Yousef Al Ayed ◽  
Mutasem Ababneh ◽  
Asirvatham Alwin Robert ◽  
Aus Alzaid ◽  
Rania Ahmed Ahmed ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Saudi Arabia ◽  
Diabetic Foot ◽  
Antibiotic Sensitivity ◽  
Foot Ulcers ◽  
Diabetic Foot Ulcers ◽  
Gram Positive ◽  
Gram Negative ◽  
Klebsiella Spp

We sought to define the type and antibiotic sensitivity response of pathogens isolated from diabetic foot ulcers based on ulcer location and Wagner classification. This retrospective analysis was done among 126 patients between 40 and 70 years of age at the Prince Sultan Military Medical City, Riyadh, Saudi Arabia, using the swab culture method for microbiological assessment. Data on demographic variables, ulcer sites (location), and depth of ulcer (Wagner classification) were also collected. A total of 134 pathogens were isolated from 126 patients. The most common gram-negative pathogen identified was Pseudomonas aeruginosa (15.6%), followed by Klebsiella spp (6.7%). The most common gram-positive pathogen was Staphylococcus aureus (35%), followed by Streptococcus (8.9%). The most widely accepted antibiotic to which the gram-negative bacteria were sensitive was gentamycin (20.1%), followed by ciprofloxacin (19%). The most routinely used antibiotic in the treatment of the gram-positive bacteria was erythromycin (16%), with trimethoprim-sulfamethoxazole (14.1%) being the next most effective. In conclusion, Pseudomonas aeruginosa, Klebsiella spp, Staphylococcus aureus, and Streptococcus were the most common causes of diabetic foot infections. However, the frequency of encountered pathogens and respective sensitivity to antibiotic therapy may be influenced by the location site and severity of the ulceration.

DISTRIBUTION OF HEAT-LABILE AND HEAT-STABLE INORGANIC PYROPHOSPHATASES AMONG SOME BACTERIA

1967 ◽  
Vol 13 (12) ◽  
pp. 1695-1699 ◽  
Author(s):  
Bernard I. Blumenthal ◽  
Mary K. Johnson ◽  
Emmett J. Johnson
Keyword(s):  
Staphylococcus Aureus ◽  
Gram Positive ◽  
Gram Negative ◽  
Heat Stable ◽  
Mycobacterium Phlei ◽  
Heat Labile ◽  
Micrococcus Lysodeikticus ◽  
Gram Positive Cocci

Significant biochemical differences were found in the inorganic pyrophosphatases from different bacteria. All of the Gram-positive sporeforming rods tested possessed a constitutive, heat-labile enzyme, and all of the Gram-negative rods tested possessed a constitutive, heat-stable enzyme. Of the enzymes from the Gram-positive cocci studied, the enzymes from two species of the genus Streptococcus were constitutive and heat labile, the enzyme from Micrococcus lysodeikticus was inducible and heat stable, and that from Staphylococcus aureus was inducible and heat labile. The enzyme from Mycobacterium phlei, the only acid-fast organism studied, was constitutive and heat labile.

scholarly journals Bacteriology of diabetic foot infections and their antibacterial susceptibility

2018 ◽  
Vol 6 (10) ◽  
pp. 3276
Author(s):  
Alex Aiswariya ◽  
Kalagara Pavani ◽  
Bhanudas Surpam Rajendra
Keyword(s):  
Diabetic Foot ◽  
Bacterial Infections ◽  
Multidrug Resistant ◽  
Gram Positive ◽  
Gram Negative ◽  
Clinical Specimens ◽  
Antimicrobial Susceptibility Pattern ◽  
Diabetic Foot Infections ◽  
Microbiological Techniques ◽  
Gram Positive Cocci

Background: Diabetic foot infections are the most common bacterial infections encountered in patients with diabetes mellitus and remain the leading complication requiring frequent hospitalization.  Hence, this study was carried out to determine the prevalence of bacteria in diabetic foot infections and their antibiogram which can help to inform therapeutic choices.Methods: A prospective study conducted on clinical specimens taken from patients with diabetic foot infections, over 3 years duration. The clinical specimens were processed by using the standard microbiological techniques. The antimicrobial susceptibility pattern was studied by the Kirby-Bauer disc diffusion method.Results: Among 103 cases studied, 97 were culture positive. Out of these specimens, 25 (25.77%) had monomicrobial flora and 72 (74.23%) had polymicrobial flora. A total of 176 bacteria were obtained which include 62 gram positive cocci and 114 gram negative bacilli. All gram negative bacilli showed good sensitivity to imipenem (97.30%), followed by cefaperazone sulbactam (81.98%), piperacillin-tazobactam (75.68%) and amikacin (72.97%). All gram positive cocci remained 100% sensitive to Vancomycin and Linezolid followed by clindamycin (not tested for Enterococci Spp.) and gentamicin  in a range of  91.43 % to 72.88%. The prevalence of multidrug resistant organisms among aerobic isolates were 59.66%.Conclusions: Diabetic foot infections are polymicrobial. Pseudomonas aeruginosa and Staphylococus aureus were the most common isolates. The most sensitive antibiotics are imipenem for gram negative bacilli while vancomycin and linezolid were effective for gram positive cocci. The antibiogram of isolates will be helpful in determining the drugs for the empirical treatment of diabetic foot infection.

scholarly journals Study of Aerobic Bacterial Etiology and Their Antibiogram from Pus Samples in a Tertiary Care Hospital

2021 ◽  
Vol 8 (4) ◽  
pp. 358-362
Author(s):  
T. Kanakadurgamba ◽  
Rama Lakshmi Koripella ◽  
B. Gowtham
Keyword(s):  
Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Microbiological Analysis ◽  
Gram Positive ◽  
Gram Negative ◽  
Culture Positive ◽  
Gram Positive Cocci

Introduction: The spread of multidrug resistant bacteria has added a new angel to the problem of wound infections which are a major cause of morbidity. To avoid difficulty in treatment of such infections it is mandatory for every pus sample to undergo Culture and sensitivity. Aim: To isolate and study the antibiogram of the aerobic bacteria from pus samples. Materials and Methods: A total of 426 pus samples received from various wards in the Microbiology department. Andhra Medical College, Visakhapatnam were processed as per standard guidelines in the laboratory. All the aerobic bacterial isolates obtained were kept for antibiogram by Kirby Bauer’s disc diffusion method as per CLSI Guidelines. Results: Out of 426 samples 394 (92.4%) were culture positive. Among culture positive samples 90.6% were pure cultures and 9.4% were mixed isolates. Gram negative bacilli were 72.8% of the total isolates and 27.2% were Gram positive cocci. Escherichia coli and Klebsiella pneumonia were the predominant isolates (30.3% and 28.9%) followed by Pseudomonas aeruginosa (24%), Proteus species (9.7%) and Acinetobacter species (6.9%). Among Gram positive cocci Staphylococcus aureus (62.6%), Enterococci (20.5%) and Coagulase Negative Staphylococcus (11.2%) and Streptococci (3.7%). GNB were more sensitive to Colistin (92%), Amikacin (86%), Imipenem and Meropenem (86% and 84.4%), Piperacillin Tazobactam (84%), Gentamycin (78%), Cefaperazone Sulbactam (76.2%) and Ciprofloxacin (56%). Gram positive cocci were sensitive to Linezolid and Teicoplanin (98% each), Vancomycin (96.2%), Clindamycin (84%), Gentamycin (79%), Azithromycin (76.4%), Ciprofloxacin (66%) and Amoxiclav (54%) out of 62.6% Staphylococcus aureus isolates 31.4% were MRSA and out of 72.8% of Gram negative bacilli 38.2% were ESBLS. Conclusion: As multidrug resistant bacterial strains are emerging in various infectious and pyogenic wound infection is most important cause of morbidity, it is important for a clinician to send all pus samples for microbiological analysis and their antibiogram before putting cases on antibiotics. Keywords: Pus samples, Multidrug resistance, Gram negative bacilli, Gram positive cocci, Antimicrobial sensitivity.

scholarly journals Assessment of the Bacterial Pollution and Detection of Antibiotic Resistance Genes in Benin: Case of the Hydrographic Channel Complex Cotonou-Nokoué Lake

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Oloufemi Daniel Ichola ◽  
Victorien Tamegnon Dougnon ◽  
Charles Hornel Koudokpon ◽  
Alidehou Jerrold Agbankpe ◽  
Esther Deguenon ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Water Samples ◽  
Bacterial Species ◽  
Resistant Bacteria ◽  
Biochemical Tests ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Cocci

The study aims to document the level of contamination of the aquatic ecosystem of the Cotonou-Lake Nokoué canal hydrographic complex by multidrug-resistant bacteria and their resistance genes. For this purpose, water samples were taken from several points of the complex and from the sediments at the depth of the lake. Samples of several species of freshly caught fish products from the lake were also collected. Bacteriological analyses were carried out according to the AFNOR standard (NF U: 47–100). The identification of the different bacterial species isolated was then carried out using the API 20E gallery and specific biochemical tests. The antibiogram of the strains was performed according to the recommendations of the EUCAST. Molecular characterization of the identified strains was carried out by searching for resistance and virulence genes. The results obtained revealed the presence of several bacterial species in water samples and in sediment and intestine samples of fishery products with a predominance of Gram-negative bacilli. The resistance profile of Gram-negative bacilli showed a total resistance to metronidazole (100%). 23% of the strains were also resistant to ciprofloxacin, 41% to amoxicillin, and 60% to aztreonam. Of the Gram-positive cocci identified, 66% was resistant to vancomycin, 7.5% to ciprofloxacin, 71% to erythromycin, and 22% to tetracycline. Regarding the genes sought, blaTEM (46%), blaSHV (24%), and blaCTX-M-15 (31%) were present in the genome of Gram-negative bacilli as resistance genes and fimH (41%) as virulence gene. As for Gram-positive cocci, the van B gene was completely absent. The van A was present at 6.25% in Staphylococcus aureus and mecA at 21.88 and 33.33%, respectively, in Staphylococcus aureus and coagulase-negative staphylococci strains. The high resistance of isolated bacterial strains is a matter of concern and calls for a rational use of antibiotics in order to avoid the transmission of antibiotic resistance from the environment to humans.

scholarly journals Isolation, Enumeration, and Identification of Gram-Positive Cocci from Frozen Crabmeat

1995 ◽  
Vol 58 (8) ◽  
pp. 853-857 ◽  
Author(s):  
RUDOLPH D. ELLENDER ◽  
LIEPIN HUANG ◽  
SANDRA L. SHARP ◽  
ROBERT P. TETTLETON
Keyword(s):  
Staphylococcus Aureus ◽  
Species Identification ◽  
Incubation Temperature ◽  
Identification Procedure ◽  
Gram Positive ◽  
Gram Negative ◽  
Plate Counts ◽  
Isolation Media ◽  
Bacterial Type ◽  
Gram Positive Cocci

Bacterial levels in frozen crabmeat samples were determined by plate counts using four staphylococcal isolation media incubated for 24, 48, and 72 h at 26 and 35°C. Staphylococcal counts determined by the spread-plate Food and Drug Administration Baird-Parker protocol incubated at 35°C for 48 h (FDABP48-35) served as the standard for comparison. When FDABP48-35 counts were compared to counts from 29 combinations of media, time of incubation, and incubation temperature, only FDABP and Borrego, Florido, Mrocek, and Romero (BFMR) counts, representing 11 combinations, were statistically comparable to FDABP48-35 counts. Cocci (91.5%) were the dominant bacterial type; gram-positive rods (8.3%) and gram-negative isolates (0.2%) were also detected. Isolates tested by the coagulase reaction were predominantly coagulase negative (CN) (97.7%). Of 100 isolates analyzed by the BIOLOG identification procedure, 62% were classified as Staphylococcus lentus, S. hominis, and S. epidermidis. Three isolates were identified as Staphylococcus aureus. These data indicate that species identification of staphylococci from crabmeat can assist in determining the source of contamination, and that staphylococcal isolates from crabmeat are more likely to be coagulase negative.

Bacterial Diversity, Antibiotic Resistance, and the Risk of Lower Limb Amputation in Younger and Older Individuals With Diabetic Foot Infection

2021 ◽  
pp. 153473462199229
Author(s):  
Stefan Dörr ◽  
Ann-Kathrin Holland-Letz ◽  
Gregor Weisser ◽  
Apostolos Chatzitomaris ◽  
Ralf Lobmann
Keyword(s):  
Bacterial Diversity ◽  
Diabetic Foot ◽  
Care Center ◽  
Bacterial Load ◽  
Limb Amputation ◽  
Older Individuals ◽  
Foot Care ◽  
Gram Positive ◽  
Gram Negative ◽  
Diabetic Foot Infection

Introduction A diabetic foot infection (DFI) contributes to high mortality and morbidity in diabetics due to its often rapid progressive and intricately treatable infection. DFIs are usually a polymicrobial infection and characterizing the entire bacterial load is still challenging. Prompt and effective treatment of DFI is nevertheless mandatory to safe limbs and lives. It is therefore crucial to know the local pathogen spectrum and its antibiotic susceptibility. Methods and Material For a 12-month period, we investigated 353 individuals with infected diabetic foot ulcer, their bacterial diversity, and antimicrobial susceptibility at fist-time visit in a Diabetic Foot Care Center in southern Germany. Results Cultures yielded 888 species, most of them gram-positive cocci (primary Staphylococcus aureus). The gram-negative sector was mainly formed by Pseudomonas aeruginosa and Enterobacteriacae. Because the prevalence of multiresistant species was surprisingly low (0.9% of isolated strains), we suggest penicillins with β-lactamase inhibitor in case of gram-positive–dominated infection or piperacillin/tazobactam or rather carbapenems with equal efficacy when gram-negative species are involved.

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