scholarly journals Morphoanatomical study, seasonal variation, and larvicidal activity of volatile oils from the leaves of Campomanesia pubescens (DC.) O. Berg (Myrtaceae)

2021 ◽  
Vol 10 (3) ◽  
pp. e35610313412
Author(s):  
Henrique Margues da Costa ◽  
Tatiana de Sousa Fiuza ◽  
Heleno Dias Ferreira ◽  
Fernando Yano Abrao ◽  
Camila Aline Romano ◽  
...  

The aim of this study was to perform from the morpho-anatomical study, physicochemical characterization, chemical composition, seasonal variability and larvicidal activity of the volatile oils of Campomanesia pubescens (DC.) O. Berg leaves. The botanical material was collected in Hidrolândia, Goiás. Morpho-anatomical characterization and phytochemical screening were performed by conventional methods. Leaf powders were submitted to hydrodistillation in the Clevenger apparatus and the identification of the chemical components of the volatile oils obtained was done by GC-MS. The larvicidal activity was carried out with third-stage larvae of the Aedes aegypti. The leaf blade, the main vein, and the young stem have a uni-stratified epidermis covered by a cuticle. The mesophyll and cortical parenchyma of the main vein and young stem have secretory cavities and idioblasts with crystals. The main rib contains a bicolateral bundle in an open arch. The young stem has a discontinuous band of sclerenchyma external to the phloem cells. Most volatile oil compounds are spathulenol, caryophyllene oxide, α-macrocarpene, and z-caryophylleneIn phytochemical analysis, tannins, digitalis, flavonoids, and total phenols were detected. The content of volatile compounds was 7.36%, that of total ash was 1.77%, and that of mucilage was 3.52 ml. The volatile oil at the concentration used was inactive against Ae aegypti larvae. The present study contributes to the taxonomic knowledge of the species and provides parameters for quality control of the plant raw material. This work represents the first description of the chemical compounds and seasonal variability of volatile oils from C. pubescens leaves collected in Goiás state.

2021 ◽  
Vol 10 (11) ◽  
pp. e34101119354
Author(s):  
Liliane de Sousa Silva ◽  
Christiane França Martins ◽  
Fernando Yano Abrão ◽  
Camila Aline Romano ◽  
Soraia Ferreira Bezerra ◽  
...  

Eugenia punicifolia (Kunth) D.C., Myrtaceae, known as “pedra-ume-caá”, is popularly used in the treatment of inflammation, infections, fever, flu, diabetes, and diarrhea. This study aimed to carry out a comparative study of the chemical composition of volatile oil from E. punicifolia leaves collected in Goiás and Minas Gerais, as well as to evaluate the larvicidal activity against Aedes aegypti L3 larvae, the antimicrobial activity against bacteria, pathogenic fungi, and environmental, and cytotoxic activity to Balb 3T3 cells (murine fibroblasts). Volatile oils were obtained by hydrodistillation in a Clevenger apparatus and analyzed by Gas Chromatography Coupled to Mass Spectrometry (CG/MS). A total of 60 compounds were identified, the main components found in the leaves of Goiás being Germacrene D, bicyclogermacrene and β-longipenene and in the leaves collected in Minas Gerais they were (Z)-caryophyllene, γ-cadinene, spathulenol, caryophyllene oxide, and α-cadinol. The larvicidal effect was moderate against Ae. aegypti, with LC50 of 85.53 µg / mL for samples from Goiás and LC50 of 91.52 µg / mL for samples from Minas Gerais. Both oils showed moderate bactericidal activity against K. rhiziphyla (ATCC 9341), M. luteus (ATCC 10240), and S. aureus (ATCC 29737). The oils from Goiás (IC50 706.7 µg / mL) and Minas Gerais (IC50 160.7 µg / mL) had a lower cytotoxic concentration than the toxic action for larvae and bacteria, evidencing a safety profile and an interesting therapeutic potential, mainly concerning to volatile oil from Goiás. Therefore, the volatile oils from E. punicifolia leaves collected in Goiás and Minas Gerais that presented moderate larvicidal activity for Ae. aegypti also presented a bactericide activity and less cytotoxicity against murine fibroblasts. This is the first study of the larvicidal, antimicrobial and cytotoxic activity of volatile oils from E. punicifolia leaves.


OCL ◽  
2020 ◽  
Vol 27 ◽  
pp. 42
Author(s):  
Charlène Hubert ◽  
Cristelle Meriadec ◽  
Pascal Panizza ◽  
Franck Artzner ◽  
Hélène de Clermont-Gallerande

The long-lasting make-up was created to avoid migration of the product on the skin throughout the day. Currently, the formulation technology most commonly used to obtain it is based on the use of film-forming agents with volatile oils. However, with the growing trend in natural cosmetics, petroleum-based volatile compounds are increasingly being abandoned. The objective of this study is to determine whether a mixture of plant-based butters can be chosen to replace a mixture of volatile oil and synthetic wax in a long-lasting eyeshadow. Initial comparisons were made between two jojoba butters and a wax/volatile oil mixture in order to identify the best candidate to replace the latter. Then, the best candidate was incorporated into formula and finished formulas were compared using sensory evaluation, rheology and X-ray scattering. The results show that it is possible to preserve texture, rheological properties and structural organization of a formula by replacing a wax/oil mixture with vegetable butters. However, this does not mean that the formulas will behave the same on the eyelids over time. This work illustrates the real complexity faced by formulators when they must replace one raw material with another while guaranteeing the durability of all the product’s properties. This challenge is more relevant today as consumer demand for products based on natural ingredients is growing.


2021 ◽  
Vol 10 (6) ◽  
pp. e49110615929
Author(s):  
Fernando Yano Abrao ◽  
Henrique Marques da Costa ◽  
Tatiana de Sousa Fiuza ◽  
Camila Aline Romano ◽  
Heleno Dias Ferreira ◽  
...  

Psidium guineense Swartz is a bush used in urinary tract diseases, diarrhea, and dysentery. The present study aims to perform the anatomical study of the leaves and evaluation of the chemical composition of the volatile oils from Psidium guineense Swartz leaves and fruits. The botanical material was collected in Hidrolândia, Goiás. Anatomical characterization and phytochemical screening of the leaves were performed by conventional methods. Leaf and fruit (green fruits, immature fruits, and ripe fruits) powders were submitted to hydrodistillation in the Clevenger apparatus and the identification of the chemical components of the volatile oils obtained was done by GC-MS. The leaf blade is hypoestomatic with paracytic and anisocytic stomata. Secretory cavities are observed in the central vein, mesophyll, petiole, and young stem. The powder moisture content was 7.4%. The total ash content of the leaf powder was 6.3% and the acid-insoluble ash content was 0.8%. The presence of tannins, flavonoids, and saponins in the leaves were identified. Leaves volatile oil’s majority compounds were 2Z,6E-farnesol (23.1-25.4%), α-copaene (17.7-20.3%), muurola-4,10(1.4) dien-1-β-ol (5.8-6.7%), epi-α-cadinol (5.5- 6.3%), and δ-Cadinene (5.0- 5.9%). Fruits volatile oil’s majority compounds were 2Z,6E-farnesol (31.9-41.4%), α-copaene (13.3-26.6%), δ-cadinene (5.4-9.8%), γ-himachalene (3.8-6.1%), and cubenol (2.6-6.1%). This is the first report on anatomical study of the leaves, and chemical composition of volatile oils from leaves and fruits of P. guineense collected in Hidrolândia, Goiás.


2021 ◽  
Vol 117 (2) ◽  
pp. 1
Author(s):  
Amir SOLTANBEIGI ◽  
Harun DIRAMAN ◽  
Mohammad Bagher HASSANPOURAGHDAM

<p>Volatile oil and fatty acids components of six various populations of wild Bunium persicum Boiss. (Bam and Zirkuh/Iran) and cultivated Cuminum cyminum L. (Rayen/Iran; Cukurcak, Taskopru and Asagialicomak/Turkey) species were investigated. The volatile oil content of Bam and Zirkuh populations were 3.9 and 4.7 %, respectively. The analysis of volatile oils by the GC/FID-MSD showed that γ-terpinene (33.62-39.62 %), cuminal (17.9-19.3 %), o-cymene (5.3-11.1 %), benzenemethanol, α-methyl- (7.4-9.5 %), 1-phenyl-1-butanol (6.4-8.4 %) and limonene (6.4-8.6 %) were the major components of B. persicum populations. Rayen, Cukurcak, Taskopru and Asagialicomak populations of C. cyminum had 2.6, 2.2, 2.0 and 2.5 % of volatile oil, respectively. Cuminal (22.8-37.6 %), benzenemethanol, α-methyl- (5.3-22.6 %), γ-terpinene (16.7-19.4 %), β-pinene (11.2-11.9 %) and 1-phenyl-1-butanol (5.4-12.5 %) were identified as the main components of C. cyminum. Fatty acids were detected by the GC/FID. In total, 15 fatty acids were characterised in B. persicum populations from Iran. Petroselinic acid (26.3-52.6 %), lauric acid (16.2-37.0 %) and linoleic acid (18.3-33.0 %) were the predominant fatty acids identified in Iranian populations. C. cyminum populations were rich in the same fatty acids but, the order was: petroselinic acid (47.5-55.5 %), linoleic acid (22.5-25.4 %) and lauric acid (13.4-24.2 %). Monounsaturated fatty acids (27.4-56.2 %) were the major subgroup. Overall, B. persicum populations from Iran and C. cyminum from Turkey were almost similar in fatty acids profile although they had wide diversity in the volatile oils compositional profile.</p>


Author(s):  
Sunday Faith Oyelere ◽  
David Adesoye Tunwagun ◽  
Makinde Victor Bamikunle ◽  
Titilayo Eunice Ayoade ◽  
Temitope Aderonke Adebayo ◽  
...  

This study assessed the phytochemical contents of the aqueous, ethanol, ethyl acetate and methanol extracts of the fruit, leaves, root, and stem of F. thonningii at selected concentrations. The contents of alkaloids, tannins, saponins, volatile oils, phenols and flavonoids were determined qualitatively in these extracts at selected concentrations. The results obtained showed that alkaloids are present in the leaves, roots and stem barks of Ficus thonningii. The volatile oil is found in the stem bark, root bark, and fruit (aqueous only) of the plant. Saponin is found to be concentrated in all the extracts of the plant. Phenol is found to be concentrated in the fruit of the plant. Its presence is also confirmed in the leaves (at few concentrations) and present in trace amount in the stem bark. Tannin is found in the fruit, root bark and leaves of Ficus thonningii.  Flavonoids are found in all the parts of Ficus thonningii. The variety of phytochemicals confirmed in the fruit, leaves, stem, and root barks of Ficus thonningii show that the plant is pharmacologically active.


2018 ◽  
Vol 69 (9) ◽  
pp. 2338-2342
Author(s):  
Mariana Popescu ◽  
Diana Puiu ◽  
Anca Daniela Raiciu

The study describes the composition of volatile oils obtained by steam distillation of water fir (Abies alba), pine (Pinus sylvestris), juniper (Juniperus communis), rosemary (Rosmarinus officinalis), sage (Salvia officinalis), and coriander (Coriandrum sativum) using the gas chromatography technique coupled with mass spectrometry (GC-MS). Chromatographic profile revealed the presence of specific components for each studied volatile oil. It noted the presence of a and b- pinene in all volatile oils studied at different concentrations. Volatile oils studied, from green sources and rich in a, b-pinene will be used as raw materials for creating products with anti-inflammatory, antiseptic, bronchodilators properties.


Author(s):  
Gilberto Gonçalves Facco ◽  
Eloty Justina Schleder ◽  
Natalia Yoshioka de Vidis ◽  
Maristela Halverson ◽  
Rosemary Matias ◽  
...  

This study aimed to carry out morphoanatomical, histochemical and phytochemical analysis of leaves and stems of Tetrapterys multiglandulosa Cav. (Malpighiaceae), a native plant responsible for abortion and sudden death in beef cattle. Plant specimens underwent anatomical study, in which leaves and stems were dried and ground with aqueous and ethanol extracts subjected to histochemical and phytochemical analysis. Anatomical observation of vegetative organs detected the presence of simple columnar cells in the adaxial epidermis, palisade mesophyll, parenchyma with a layer of cells and paracitic type stomata. Stem cross-sections presented circular section, single layer (uniseriate) epidermis with thick cuticle and Malpighi’s trichomes, the phelogen was in differentiation. The cortex showed angular collenchyma, parenchyma with exogenous channels and druse shaped calcium oxalate crystals. Phytochemical tests of aqueous and ethanol extracts of leaves and stems indicated the presence of phenolic compounds, tannins, flavonoids, saponins, alkaloids and cardiotonic heterosides. Potentially phytotoxic secondary metabolites evidenced in the leaves and stems, including saponins and cardiotonic heterosides were correlated with clinical signs observed in intoxicated animals.


Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 495
Author(s):  
Shixing Zhou ◽  
Toshmatov Zokir ◽  
Yu Mei ◽  
Lijing Lei ◽  
Kai Shi ◽  
...  

The chemical profile and allelopathic effect of the volatile organic compounds (VOCs) produced by a dominant shrub Serphidium kaschgaricum (Krasch.) Poljak. growing in northwestern China was investigated for the first time. Serphidium kaschgaricu was found to release volatile compounds into the surroundings to affect other plants’ growth, with its VOCs suppressing root elongation of Amaranthus retroflexus L. and Poa annua L. by 65.47% and 60.37% at 10 g/1.5 L treatment, respectively. Meanwhile, volatile oils produced by stems, leaves, flowers and flowering shoots exhibited phytotoxic activity against A. retroflexus and P. annua. At 0.5 mg/mL, stem, leaf and flower oils significantly reduced seedling growth of the receiver plants, and 1.5 mg/mL oils nearly completely prohibited seed germination of both species. GC/MS analysis revealed that among the total 37 identified compounds in the oils, 19 of them were common, with eucalyptol (43.00%, 36.66%, 19.52%, and 38.68% in stem, leaf, flower and flowering shoot oils, respectively) and camphor (21.55%, 24.91%, 21.64%, and 23.35%, respectively) consistently being the dominant constituents in all oils. Eucalyptol, camphor and their mixture exhibited much weaker phytotoxicity compared with the volatile oils, implying that less abundant compounds in the volatile oil might contribute significantly to the oils’ activity. Our results suggested that S. kaschgaricum was capable of synthesizing and releasing allelopathic volatile compounds into the surroundings to affect neighboring plants’ growth, which might improve its competitiveness thus facilitate the establishment of dominance.


Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 792
Author(s):  
Kati Väkeväinen ◽  
Noora Rinkinen ◽  
Roosa-Maria Willman ◽  
Jenni Lappi ◽  
Kaisa Raninen ◽  
...  

Blackcurrant is a healthy, affordable, and traditionally gardened berry that, thus far, has been underused in food applications. From the consumers’ point of view, the acidic taste of blackcurrants is a challenge; therefore, these berries have mainly been utilized for sugary juice production. This research study aimed to develop a frozen vegan blackcurrant product with pleasant sensory properties and potential probiotic function. A candidate probiotic, Lactoplantibacillus plantarum Q823, was used in the manufacturing process. The physicochemical properties, nutritional composition, and consumer preference for the developed product were assessed, as was the viability of L. plantarum Q823 during storage time and in an in vitro gastrointestinal model. Consumers (n = 71) perceived the developed product to be pleasant. L. plantarum Q823 had high viability counts (log colony forming units (cfu) g−1 7.0 ± 0.38) in the final product, although the viability of L. plantarum Q823 during storage time needs to be enhanced to obtain a probiotic product. Thus, within an optimized formulation, blackcurrant berries represent a potential raw material for functional frozen food products.


Forests ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 447
Author(s):  
Miguel Alfonso Quiñones-Reveles ◽  
Víctor Manuel Ruiz-García ◽  
Sarai Ramos-Vargas ◽  
Benedicto Vargas-Larreta ◽  
Omar Masera-Cerutti ◽  
...  

This study aimed to evaluate and compare the relationship between chemical properties, energy efficiency, and emissions of wood and pellets from madroño Arbutus xalapensis Kunth, tázcate Juniperus deppeana Steud, and encino colorado Quercus sideroxyla Humb. & Bonpl. in two gasifiers (top-lit-up-draft (T-LUD) and electricity generation wood camp stove (EGWCS)) in order to determine the reduction of footprint carbon. In accordance with conventional methodologies, we determined the extracts and chemical components (lignin, cellulose, holocellulose), and the immediate analyses were carried out (volatile materials, fixed carbon, ash content and microanalysis of said ash), as well as the evaluation of emission factors (total suspended particulate matter (PM2.5), CO, CO2, CH4, black carbon (BC), elemental carbon (EC), and organic carbon (OC)). The results were statistically analyzed to compare each variable among species and gasifiers. The raw material analyzed showed how the pH ranged from 5.01 to 5.57, and the ash content ranged between 0.39 and 0.53%. The content values of Cu, Zn, Fe, Mg, and Ca ranged from 0.08 to 0.22, 0.18 to 0.19, 0.38 to 0.84, 1.75 to 1.90, and 3.62 to 3.74 mg kg−1, respectively. The extractive ranges from cyclohexane were 2.48–4.79%, acetone 2.42–4.08%, methanol 3.17–7.99%, and hot water 2.12–4.83%. The range of lignin was 18.08–28.60%. The cellulose content ranged from 43.30 to 53.90%, and holocellulose from 53.50 to 64.02%. The volatile material range was 81.2–87.42%, while fixed carbon was 11.30–17.48%; the higher heating value (HHV) of raw material and pellets presented the ranges 17.68–20.21 and 19.72–21.81 MJ kg−1, respectively. Thermal efficiency showed statistically significant differences (p < 0.05) between pellets and gasifiers, with an average of 31% Tier 3 in ISO (International Organization for Standardization) for the T-LUD and 14% (ISO Tier 1) for EGWCS, with Arbutus xalapensis being the species with the highest energy yield. The use of improved combustion devices, as well as that of selected raw material species, can reduce the impact of global warming by up to 33% on a cooking task compared to the three-stone burner.


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