scholarly journals Antibacterial activity of crude extract of Tabernaemontana catharinensis latex (A. DC) against Alicyclobacillus spp.

2021 ◽  
Vol 10 (9) ◽  
pp. e16310917907
Author(s):  
Fabiana Richard ◽  
Márcia Maria dos Anjos Szczerepa ◽  
Kathielle Luiza Mucellini ◽  
Érica Benassi Zanqueta ◽  
César Armando Contreras Lancheros ◽  
...  
Keyword(s):  
Orange Juice ◽  
Food Industry ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Biological Activities ◽  
Antimicrobial Effect ◽  
Vero Cells ◽  
The Family ◽  
Food And Beverage ◽  
Citrus Juices

Alicyclobacillus spp. is composed of Gram-positive, aerobic, thermoacidophilic, endospore-forming bacteria that cause food and beverage spoilage. The presence of Alicyclobacillus spp. may result in the production of guaiacol, which leads to sensory changes in the odour and taste of citrus juices and acidic foods. Tabernaemontana catharinensis (A. DC) is a plant belonging to the family Apocynaceae that produces milky latex with several biological activities described as antioxidant, antiviral, antimicrobial, trypanocidal and anti-leishmanicidal. Therefore, this study aims to evaluate the antimicrobial activity of the crude latex of T. catharinensis (A. DC) against microorganisms of the genus Alicyclobacillus spp. The minimum inhibitory concentration of latex was 7.81 μg/ml for the five Alicyclobacillus species analysed. The minimum bactericidal concentration for the species Alicyclobacillus acidoterrestris 0244T, A. hesperidum 0298T, A. acidiphilus 0247T and A. cycloheptanicus 0297T was 250 μg/ml. Cytotoxicity analysis demonstrated that latex was toxic to Vero cells at concentrations greater than 84.67 μg/ml. Scanning electron microscopy revealed changes in the cell wall of A. acidoterrestris 0244T present in orange juice when treated with crude latex. The results obtained suggest that the crude latex of T. catharinensis (A. DC) displays an antimicrobial effect against Alicyclobacillus, with potential for application in the food industry.

scholarly journals Chemical Components and Biological Activities of the Genus Phyllanthus: A Review of the Recent Literature

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2567 ◽  
Author(s):  
Muhammad Nisar ◽  
Junwei He ◽  
Arsalan Ahmed ◽  
Youxin Yang ◽  
Mingxi Li ◽  
...  
Keyword(s):  
Food Industry ◽  
Recent Literature ◽  
Biological Activities ◽  
Chemical Components ◽  
Cellular Functions ◽  
Underdeveloped Countries ◽  
The Family ◽  
Males And Females ◽  
Loss Of Appetite ◽  
The Tropics

Medicinal plants have served humans since prehistoric times to treat various ailments. Both developed and underdeveloped countries rely on traditional systems of medication using natural sources from plants. Phyllanthus is one of the largest genus in the family Phyllanthaceae, comprising over 700 well known species cosmopolitan in distribution mainly in the tropics and subtropics. Phyllanthus species are being in constant used in traditional medications to cure an array of human diseases (constipation, inhalation related, arthritis, loss of appetite, injuries, conjunctivitis, diarrhoea, running nose, common cold, malaria, blennorrhagia, colic, diabetes mellitus, dysentery, indigestion, fever, gout, gonorrheal diseases of males and females, skin itching, jaundice, hepatic disorders, leucorrhea, vaginitis, menstrual irregularities, obesity, stomach pains, and tumors), confectionaries, food industry, and in some pesticides. Phyllanthus species are rich in diversity of phytochemicals e.g., tannins, terpenes, alkaloids, glycosidic compounds, saponins, and flavones etc. More in depth studies are a direly needed to identify more compounds with specific cellular functions to treat various ailments.

scholarly journals Schinus terebinthifolius (Anacardiaceae) essential oil for minas frescal cheese preservation

2020 ◽  
Vol 9 (11) ◽  
pp. e1619119760
Author(s):  
Valquíria de Fátima Simões ◽  
Kátia Castilho de Oliveira ◽  
Wanessa de Campos Bortolucci ◽  
José Eduardo Gonçalves ◽  
Maria Graciela Iecher Faria ◽  
...  
Keyword(s):  
Essential Oil ◽  
Food Industry ◽  
Inhibitory Concentration ◽  
Chemical Compounds ◽  
Antimicrobial Effect ◽  
Schinus Terebinthifolius ◽  
Plant Essential Oils ◽  
Potential Source ◽  
Pepper Fruit ◽  
The Impact

Microorganism control in food has been a challenge due to the legislation restrictions of each country and the increase in the demand for food without chemical preservatives. Plant essential oils have antimicrobial action and are promising for the use in the food industry. Our study aimed to evaluate the chemical composition and the antimicrobial effect of Schinus terebinthifolius Raddi fruit essential oil on minas frescal cheese. The essential oil was extracted by hydrodistillation, and the chemical compounds were identified by gas chromatography coupled to mass spectrometry. The major identified compounds were hydrocarbon monoterpenes (57.0%), mainly: α-pinene (22.2%), limonene (17.0%), carvone (10.2%) and β-phellandrene (7.9%). The cheese was produced, the main microorganisms were isolated, and the minimum inhibitory concentration was determined through broth microdilution test.  The cheese samples were soaked. The pink pepper fruit essential oil was added to the cheese samples at the concentration of 20000 μL/mL superficially or to the micelle. S. terebinthifolius fruit essential oil was efficient to control minas frescal cheese microorganisms mainly when applied by superficial addition. The essential oil is a potential source of studies to develop applications for the control of microorganisms in cheese such as minas frescal. Further studies may exploit the impact of the essential oil in minas frescal cheese acceptability.

scholarly journals Natural Phytochemicals and Biological Activities of Wild Grape (Ampelocissus martinii Planch.) Root Extract

2021 ◽  
Vol 33 (3) ◽  
pp. 545-550
Author(s):  
P. Siripipatthana ◽  
P. Srihanam ◽  
A. Sangdee
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antibacterial Activities ◽  
Root Extract ◽  
Phytochemical Content ◽  
Gram Positive Bacteria ◽  
High Antioxidant Activity

A hydromethanolic root extract of Ampelocissus martinii Planch. (A. martinii) was analyzed by standard methods for its phytochemical content, antioxidant activity, α-amylase and α-glucosidase inhibitions and antibacterial activities. The root extract exhibited the highest content of saponins, followed by phenols, proanthocyanidin and flavonoids, respectively. It showed high antioxidant activity in FRAP and CUPRAC assays. The root extract and standard Trolox had similar antioxidant activities in the DPPH and ABTS assay. It also showed much higher α-amylase and α-glucosidase inhibitory activity compared to standard acarbose. Moreover, the root extract inhibited all tested Gram-positive bacteria with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 6.25 mg/mL. These results indicate that A. martinii root can be pharmaceutically used as active ingredients to prevent bacterial infection and radical-related diseases especially diabetes.

scholarly journals PROFIL KANDUNGAN KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN BAMBAN (Donax canniformis (G. Forst.) K. Schum.) TERHADAP Staphylococcus aureus

2015 ◽  
Vol 1 (2) ◽  
pp. 141-148
Author(s):  
Hidayatullah Hidayatullah ◽  
Syariful Anam ◽  
Muhamad Rinaldhi Tandah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Minimum Bactericidal Concentration ◽  
Dilution Method ◽  
The Family

Bamban (Donax canniformis (G. Forst.) K. Schum.) is one of the family Marantaceae plant that has many uses such as traditional medicine. Methanol extract of bamban leaves contains phenolic, tannins and saponins compounds. The purpose of this research is to determine the class of compounds that has antibacterial activity against Staphylococcus aureus and determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methanol extract of bamban leaves. This extract was prepared using maceration method with methanol solvent. Determination the class of compounds was initiated by bioautografi test in order to determine spots which has have antibacterial activity. Subsequently, the spot were identified the class of compound using reagent spray FeCl3 and H2SO4 10%. The determination of MIC and MBC using dilution method. Research showed there are three compounds that had antibacterial activity. These compounds were predicted as spot I and spot II which were phenolic compounds and spot III as a saponin compound. MIC and MBC value of the methanol extract of leaves bamban leaves 8% and 13%, respectively.

scholarly journals Essential Oil of Croton ceanothifolius Baill. Potentiates the Effect of Antibiotics against Multiresistant Bacteria

Antibiotics ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
Ana C. J. de Araújo ◽  
Priscilla R. Freitas ◽  
Cristina Rodrigues dos Santos Barbosa ◽  
Débora F. Muniz ◽  
Janaína Esmeraldo Rocha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Positive Strain ◽  
Multiresistant Bacteria ◽  
The Family ◽  
Multiresistant Strains

This study is a pioneer in reporting the antibacterial properties of the species Croton ceanothifolius Baill. The genus Croton belongs to the family Euphorbiaceae composed of numerous species with documented biological activities. However, the pharmacological properties of C. ceanothifolius remain poorly understood. The leaves of this plant were submitted to hydrodistillation for essential oil (CcEO) extraction and the phytochemical characterization of the oil was performed by GC/MS. The minimum inhibitory concentration of the CcEO was determined for the evaluation of antibacterial activity against multiresistant strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The antibiotic-modulating activity of the oil, in combination with antibiotics, was also evaluated. The combination of the CcEO with penicillin, norfloxacin, and gentamicin presented a synergistic effect. This effect was more significant for the association with antibiotics of the quinolone and aminoglycoside classes against Escherichia coli. The association of oil with gentamicin showed better results with regard to the Gram-positive strain. The association of the oil with norfloxacin against P. aeruginosa also showed synergism, but the association with penicillin did not change the effect of this antibiotic. Thus, it is concluded that C. ceanothifolius essential oil selectively potentiates the action of antibiotics against multiresistant strains.

scholarly journals In vitro antimicrobial activity of total extracts of the leaves of Petiveria alliacea L. (Anamu)

2013 ◽  
Vol 49 (2) ◽  
pp. 241-250 ◽  
Author(s):  
Ania Ochoa Pacheco ◽  
Jorge Marín Morán ◽  
Zenia González Giro ◽  
Adrian Hidalgo Rodríguez ◽  
Rachel Juliet Mujawimana ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Isopropyl Alcohol ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antimicrobial Effect ◽  
Bacterial Strains ◽  
Chemical Parameters ◽  
Petiveria Alliacea ◽  
Quality Control Parameters

The antimicrobial activity of 13 total extracts was evaluated, 10 soft extracts (B) and 3 blended extracts (E) prepared from dry and fresh leaves of Petiveria alliacea L. Various solvents were used for their preparation: hydroalcoholic solution at 30%, 80% and isopropyl alcohol. The antimicrobial effect of the extracts was tested by means of the method of Kirby-Bauer, using four bacterial strains from the ATCC collection (Escherichia coli, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa) and a leveduriform fungus (Candida albicans). The following quality control parameters were determined for most active extracts: physical, physical-chemical and chemical parameters. The results were: nine extracts showed antibacterial activity, being the most concentrated (B8 and E3), the ones with the highest activity in the presence of the bacteria tested; the effect of blended extracts (E1, E2 and E3) was greater in the presence of P. aeruginosa. Blended extracts are considered more potent and active than soft extracts. No antifungal activity was obtained for both types of extracts. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined for both extracts, with the following results: MIC-soft extracts (>100 mg/mL), blended extracts (>50 mg/mL); MBC-soft extracts (≥400 mg/mL), blended extracts (≥200 mg/mL) based on fresh leaves.

scholarly journals “Sweet Flavonoids”: Glycosidase-Catalyzed Modifications

2018 ◽  
Vol 19 (7) ◽  
pp. 2126 ◽  
Author(s):  
Kristýna Slámová ◽  
Jana Kapešová ◽  
Kateřina Valentová
Keyword(s):  
Phenolic Compounds ◽  
Broad Spectrum ◽  
Food Industry ◽  
Water Solubility ◽  
Biological Activities ◽  
Hydrolytic Activity ◽  
Flavonoid Glycosides ◽  
Physiological Effects ◽  
Flavonoid Aglycones ◽  
Citrus Juices

Natural flavonoids, especially in their glycosylated forms, are the most abundant phenolic compounds found in plants, fruit, and vegetables. They exhibit a large variety of beneficial physiological effects, which makes them generally interesting in a broad spectrum of scientific areas. In this review, we focus on recent advances in the modifications of the glycosidic parts of various flavonoids employing glycosidases, covering both selective trimming of the sugar moieties and glycosylation of flavonoid aglycones by natural and mutant glycosidases. Glycosylation of flavonoids strongly enhances their water solubility and thus increases their bioavailability. Antioxidant and most biological activities are usually less pronounced in glycosides, but some specific bioactivities are enhanced. The presence of l-rhamnose (6-deoxy-α-l-mannopyranose) in rhamnosides, rutinosides (rutin, hesperidin) and neohesperidosides (naringin) plays an important role in properties of flavonoid glycosides, which can be considered as “pro-drugs”. The natural hydrolytic activity of glycosidases is widely employed in biotechnological deglycosylation processes producing respective aglycones or partially deglycosylated flavonoids. Moreover, deglycosylation is quite commonly used in the food industry aiming at the improvement of sensoric properties of beverages such as debittering of citrus juices or enhancement of wine aromas. Therefore, natural and mutant glycosidases are excellent tools for modifications of flavonoid glycosides.

scholarly journals Antimicrobial and Antioxidant Activities of Flavonoids Extracted from Pistacia lentiscus L., Leaves

2020 ◽  
Vol 10 (1-s) ◽  
pp. 83-89
Author(s):  
Sabrina Bakli ◽  
Harzallah Daoud ◽  
Zerroug Amina ◽  
Sadrati Nouari ◽  
Bouguerra Asma ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Concentration ◽  
Solid Medium ◽  
Minimum Bactericidal Concentration ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Dilution Method ◽  
Pistacia Lentiscus ◽  
The Subject

Increasingly, the flavonoids becoming the subject of biomedical researches, and many groups have isolated and identified the structures of flavonoids possessing important biological activities. This assess investigates the antimicrobial fight and the antioxidant strengths of flavonoids extracted from Pistacia lentiscus L. leaves.  The extract was first analyzed for its phenolic content, then the flavonoids were tested for their antimicrobial capacities by the method of diffusion against fourteen bacteria, two fungi and one yeast. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of flavonoids were determined using the dilution method on solid medium, however, the antioxidant activity was evaluated through the ability of the extract to scavenge DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals and the reducing power essay. The results indicate that flavonoids extract contain other phenolics groups. On the other side, the Antibacterial tests exhibited different activities depending on the strains. Unlike, the MIC and the MBC showed that the flavonoids extract showed a best activity against Candida albicans and Vibrio cholerae. Concerning antioxidant activity, the results indicate a moderate capacity of the flavonoid extract. These preliminary results may justify the use of this plant in the treatment of some diseases and may be exploited for therapeutic purposes. Keywords: Antimicrobial, Antioxidant, Pistacia lentiscus, flavonoids, MIC, MBC.

scholarly journals Phenotypical studies of raw and nanosystem embedded Eugenia carryophyllata buds essential oil antibacterial activity on Pseudomonas aeruginosa and Staphylococcus aureus strains

2011 ◽  
Vol 1 (3) ◽  
pp. 111-118 ◽  
Keyword(s):  
Essential Oil ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antimicrobial Effect ◽  
Bacterial Strains ◽  
Qualitative And Quantitative ◽  
In Vivo Tests ◽  
And Staphylococcus Aureus

Considering the large number of the multiresistant bacterial strains and the increasing need in new antimicrobial formulation, the aim of this study was to evaluate the antipathogenic effect of E. caryophyllata essential oil on collection and recently isolated clinical strains of P. aeruginosa and S. aureus. Experimental assays were carried out by in vitro and in vivo tests. Qualitative and quantitative assessment of the antimicrobial activity was followed by the analysis of the essential oil influence on enzymatic soluble virulence factors expression. The cytotoxic effect of the vegetal extract was quantified by using HeLa cells. Nanosystem embedded essential oil was used to establish the in vivo antipathogenic effect on mice. Qualitative screening results revealed an early microbicidal effect quantified by low minimum inhibitory concentration values. The in vivo study showed a stabiliziation of the essential oil biological activities when using nanosystem embedding, that could be used for the design of proper formulations for delivery systems with antimicrobial effect.

scholarly journals Characterization of silver nanoparticles and evaluation of their antimicrobial effect on Salmonella

2020 ◽  
Vol 9 (9) ◽  
pp. e103996435
Author(s):  
Luana Virgínia Souza ◽  
Daniela Abrantes Leal ◽  
Tayara Rodrigues da Costa ◽  
Regina Célia Santos Mendonça
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Food Industry ◽  
Salmonella Enteritidis ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Synthesis Methods ◽  
Ag Nps ◽  
Two Populations

Currently, the success of nanotechnology affects several areas of science, medicine, technology and, especially, the food industry. Silver nanoparticles (Ag-NPs) stand out, with their antimicrobial effect. Salmonella is a cause of foodborne diseases and there are reports of resistant serotypes. The use of Ag-NPs is an alternative for bacterial control in foods. The aim of this study was synthesize, characterize and verify the antimicrobial activity of Ag-NPs on serotypes of Salmonella. The  size of Ag-NPs was estimated and it was possible to detect two populations of 4.7 ± 0.09 and 35.7 ± 2.12. The zeta potential was -33.7 ± 11.8 mV indicating good dispersion stability.Ag-NP antimicrobial activity was determined from minimum inhibitory concentration (MIC).The lowest MIC found was 4.7 μg·mL-1 for Salmonella Enteritidis and the highest was 27.7 μg·mL-1 for Salmonella Infantis 1 isolate.The use of Ag-NPs is promising with respect to antimicrobial activity, however, improvements in synthesis methods should be explored in order to make commercial use viable.

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