Stability of bioactive compounds, antioxidant and microbiological activity of açaí powder (Euterpe oleracea Mart.)

Açaí is a tropical fruit, typical of the Amazon and has been drawing attention due to its antioxidant capacity and high content of anthocyanins, responsible for several pharmacological and medicinal properties, as well as potential use as a natural dye. Due to the high perishability, the development of new products from the açaí power, which present greater stability to the storage time are being produced. The evaluation period was 25 days at a temperature of 25°C. The following parameters were evaluated: ascorbic acid, chlorophyll, total anthocyanins, total flavonoids, total polyphenols and antioxidant activity by ABTS●+ and DPPH● methods. Microbiological analysis were performed for Coliforms at 35°C and 45°C, Mesophiles, Staphylococcus coagulase positive, Molds, Yeasts, and Salmonella sp. The contents of ascorbic acid, total polyphenols and flavonoids differed statistically (p ˂ 0.05) from each other, throughout the storage. However, the antioxidant activity by the DPPH● method remained stable and the microbiological analysis remained within the current legislative standards. It is concluded that the açaí powder maintained its properties throughout the storage, being an excellent source of bioactive and phenolic compounds.

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Polyphenols Content and Antioxidant Activity of Bilberry Juice Obtained from Different Altitude Samples

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i29a31581 ◽

2021 ◽

pp. 218-223

Author(s):

Stoyan I. Papanov ◽

Ekaterina G. Petkova ◽

Ivan G. Ivanov

Keyword(s):

Antioxidant Activity ◽

Malignant Tumors ◽

Vitamin B1 ◽

Titratable Acidity ◽

Total Polyphenols ◽

Radical Trapping ◽

Dpph Method ◽

Total Anthocyanins ◽

Calcium Magnesium ◽

Phosphorus Iron

There are about 30 different types of blueberries. In Bulgaria, there are 4 species of genus Vaccinium - red (vitis-idaea), black (myrtillus), blue (uliginosum) and Caucasian (arctostaphylos). Bilberries are a source of carotene, vitamin B1, vitamin B2, potassium, sodium, calcium, magnesium, phosphorus, iron, vitamin D, manganese, soluble and insoluble fibres like pectin. Blueberries contain significant amounts of anthocyanidins, antioxidants and ellagic acid. The main wealth of blueberries are the antioxidants that bind free radicals and thus prevent malignant tumors. This is why blueberry is an excellent remedy for oncology. Objective of study was to investigate the content of total polyphenols, antioxidant activity and total anthocyanins of bilberry depending on altitude. Systematic approach and critical analysis of the available scientific periodicals and DPPH method for determining the antioxidant activity (radical trapping activity). It has been established a direct relationship between altitude and antioxidant activity. In the range of 900 to 1450 m the relationship between altitude and total anthocyanins is inversely proportional. After 1500 m, the dependency becomes proportional. There is no correlation between pH, total polyphenols and titratable acidity.

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Evaluation of phenolic compounds and antioxidant activity in some edible flowers

Open Agriculture ◽

10.1515/opag-2020-0087 ◽

2020 ◽

Vol 5 (1) ◽

pp. 857-870

Author(s):

Fernando Gonçalves ◽

João Carlos Gonçalves ◽

Ana Cristina Ferrão ◽

Paula Correia ◽

Raquel P. F. Guiné

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Total Phenolic ◽

Potential Health ◽

Phenolic Contents ◽

Spectrophotometric Methods ◽

Potential Source ◽

Successive Extraction ◽

Dpph Method ◽

Total Anthocyanins

AbstractRecently, edible flowers (EF) have aroused increased interest because of their aesthetic properties as well as potential health benefits related to the occurrence of some bioactive compounds. Therefore, the aim of this work was to evaluate the total phenolics, anthocyanins, flavonoids, and antioxidant activity (AOA) (following DPPH and ABTS methods) in eleven EF. The samples were subjected to three successive extraction steps using methanol, and these extracts were then analysed for the aforementioned properties using spectrophotometric methods. The obtained extracts were used for the quantification of phenolic composition and AOA. The results indicated that, among the flowers analysed in this study, red rose, pink rose, and red carnation possessed the highest total phenolic contents (27.53, 23.30, and 18.17 mg g−1 gallic acid equivalents, respectively), total anthocyanins (3.07, 1.97, and 4.47 mg g−1 catechin equivalents [CE], respectively), and AOA (12.07, 15.77, and 12.93 mg g−1 TE, respectively, as given by the DPPH method or 8.23, 9.27 and 8.00 mg g−1 TE, respectively, as given by the ABTS method). The flowers with highest flavonoids contents were red carnation, Mexican marigold, and pink rose (17.50, 16.90, and 16.57 mg g−1 CE, respectively). Cluster analysis grouped the analysed flowers into two groups, those richest in phenolics with AOA and those not so rich. Finally, some important correlations were observed between the total phenolics and the AOA. In conclusion, these flowers could represent a potential source of natural compounds with antioxidant capacity.

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Phenolic Contents and Antioxidant Potential ofCrataegusFruits Grown in Tunisia as Determined by DPPH, FRAP, andβ-Carotene/Linoleic Acid Assay

Journal of Chemistry ◽

10.1155/2013/378264 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 9

Author(s):

Farouk Mraihi ◽

Mohamed Journi ◽

Jamila Kalthoum Chérif ◽

Munevver Sokmen ◽

Atalay Sokmen ◽

...

Keyword(s):

Antioxidant Activity ◽

Linoleic Acid ◽

Natural Antioxidants ◽

Total Phenolic ◽

Frap Assay ◽

Phenolic Contents ◽

Dpph Method ◽

Total Anthocyanins ◽

Linoleic Acid Assay

Crataegusfruit is one of most important fruits in Tunisian flora. Some fruits of this genus are edible. This study was undertaken in order to examine the benefits of these fruits in human health and their composition of antioxidants including total polyphenol, flavonoids, proanthocyanidins content, and total anthocyanins. The antioxidative properties of the ultrasonic methanolic extract were assessed by different in vitro methods such as the FRAP, DPPH, andβ-carotene/linoleic acid assay. We concluded that peel fraction of red fruits possessed relatively high antioxidant activity and might be a rich source of natural antioxidants in comparison with the pulp and seed fruit extract. The results also showed that hawthorn yellow fruit presents lower amounts of phenolic content, absence of anthocyanins, and less antioxidant capacity. Most of peel and seed fractions were stronger than the pulp fractions in antioxidant activity based on their DPPH IC50, FRAP values, and results ofβ-carotene/linoleic acid. The total phenolic compounds contents were also highly correlated with the DPPH method and the FRAP assay.

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Evaluation of Anticonvulsant and Antioxidant Activity of Senna occidentalis Seeds Extracts

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i2.2400 ◽

2019 ◽

Vol 9 (2) ◽

pp. 183-187

Author(s):

Vijay Vikram Singh ◽

Jainendra Jain ◽

Arun Kumar Mishra

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Anticonvulsant Activity ◽

Future Research ◽

Maximal Electroshock ◽

Formulation Development ◽

Dpph Method ◽

Dose Dependent Fashion ◽

Seizure Model ◽

Dose Dependent

Aim: The aim of present work was to determine the anticonvulsant and antioxidant activity of Senna occidentalis L. ethanolic seed extract by different models. Methods: For evaluation of anticonvulsant activity, Pentylenetetrazole (PTZ) seizure model and Maximal electroshock (MES) seizure model were used. For antioxidant activity, (1, 1-diphenyl - 2-picryl hydrazine (DPPH) and hydrogen peroxide (H2O2) method were used. Results: The finding suggested that the ethanolic extract (EAE) of Senna occidentalis in the dose 400 mg/kg body weight posses potent anticonvulsant activity. The EAE showed anticonvulsant action in dose dependent fashion. It was observed that upon increasing the concentration of extract, it showed reduced absorbance and increased free radical inhibition, and when comparison was made with Ascorbic acid, it showed marked antioxidant property in DPPH as well as H2O2 method. The IC50 of Ascorbic acid and EAE by DPPH method were found to be 14.56 and 14.8 respectively whereas the IC50 of Ascorbic acid and EAE by H2O2 method were found that 14.3and 14.8 respectively. Conclusion: The results of the present study concluded hat the EAE of Senna occidentalis L. possesses significant antioxidant and anticonvulsant activity. The activity was in dose dependent fashion. This study will assist in future research associated with formulation development of seeds of Senna occidentalis L. Keyword: Senna occidentalis L., Anticonvulsant, Antioxidant, DPPH model

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EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

International Journal of Pharmaceutical Sciences and Medicine ◽

10.47760/ijpsm.2021.v06i08.005 ◽

2021 ◽

Vol 6 (8) ◽

pp. 73-77

Author(s):

G.SAI SRUTHI ◽

K. SPANDANA ◽

RAMANJANEYULU K ◽

HIMABINDHU J

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Leaf Extract ◽

Ethanol Extract ◽

In Vitro Assay ◽

Vitro Assay ◽

Soxhlet Apparatus ◽

Dpph Method ◽

Anti Oxidant

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

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The Content of Chlorophyll, and Antioxidant Activity of Malabar plum (Syzygium jambos) Leaves at Different Developmental Stages

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v11i2.18419 ◽

2019 ◽

Vol 11 (2) ◽

pp. 226-233

Author(s):

Ikhsanti Maliya ◽

Sri Darmanti ◽

Sri Widodo Agung Suedy

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Developmental Stages ◽

Ascorbic Acid Content ◽

Total Phenol ◽

Color Measurement ◽

Total Phenol Content ◽

Chlorophyll Contents ◽

Syzygium Jambos ◽

Dpph Method

Malabar plum [Syzygium jambos (L.) Alston.] is a tropical plant which is used as a medicinal plant, because it contains secondary metabolites, especially in the leaves. The different leaves developmental stages can affect physiological changes, especially metabolic processes, so it is suspected to affect the antioxidant content and activity. The objective of this research was to study the difference of leaves morphology, chlorophyll contents, antioxidant contents, and activity at the different leaves developmental stages. Samples were taken from Kaliboto Village, Purworejo, Central Java. The leaf color measurement was using colorimetry; determination of chlorophyll, carotenoids, ascorbic acid, and total phenol content is was using spectrophotometry; and antioxidant activity was using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Qualitative data were analyzed descriptively, while quantitative data were analyzed by ANOVA tests. The results showed that the higher level of leaves development, the higher pigment content, total phenol, and antioxidant activity ascorbic acid content in the mature leaves is lower when compared to the young and old leaves. The results of this research provide the information that can support the use of Malabar plum leaves in traditional medicinal activity and pharmaceutical industry, as well as basic information for plant breeding.

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Pigments, Polyphenols and Antioxidant Activity of Leaf Extracts from Four Wild Rose Species Grown in Sicily

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha46211061 ◽

2018 ◽

Vol 46 (2) ◽

pp. 402-409 ◽

Cited By ~ 6

Author(s):

Francesca D'ANGIOLILLO ◽

Michele Massimo MAMMANO ◽

Giancarlo FASCELLA

Keyword(s):

Antioxidant Activity ◽

Morphological Characteristics ◽

Natural Antioxidants ◽

Total Flavonoids ◽

Leaf Extracts ◽

Methanolic Extracts ◽

Dpph Method ◽

Total Anthocyanins ◽

Total Flavonoids Content ◽

Promising Source

Leaf methanolic extracts of four wild Rose species (Rosa canina L., R. corymbifera Borkh., R. micrantha Borrer ex Sm and R. sempervirens L.) grown in Sicily were analysed in order to evaluate differences among indigenous roses which could be used such as source of bioactive compounds and natural antioxidants. Leaves were harvested during two different periods (June and October) and their morphological characteristics were described. Leaf content of chlorophylls, total anthocyanins, total flavonoids, total polyphenols (Folin-Ciocalteu method) and antioxidant activity (DPPH method) were determined. Results showed that the contents of chlorophylls and carotenoids were higher in leaves of all species harvested in June than in those harvested in October. On the contrary, total anthocyanins of R. micrantha and R. corymbifera leaves were higher in October. Total polyphenol content was higher in leaves of all species were harvested in October. No difference in total flavonoids content was recorded between the two harvest periods but only among the four species. Antioxidant activity was generally high in leaf extracts of all species in both periods and, particularly, in R. micrantha leaves harvested in June. These outcomes seem to support the assessment that leaves harvested from some Sicilian wild roses may be used as promising source of healthy compounds and that their content varies with the species and harvest period.

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QUANTITATIVE STUDY OF TOTAL PHENOLIC AND ALKALOID CONTENT IN HYDROALCOHOLIC CONTENT IN RAUWOLFIA SERPENTINA

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.no1.236 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Deepak Jain ◽

Meenakshi Jain ◽

Anurekha Jain

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Radical Scavenging ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Alcoholic Extract ◽

Test Protocol ◽

Rauwolfia Serpentina ◽

Dpph Method

The objectives of this study are to screen the phytochemicals, estimate the content of flavonoid and alkaloids compounds and determine the antioxidant capacity of the Rauwolfia serpentina stem. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and alkaloids were determined by the well-known test protocol available in the literature. The hydro alcoholic extract of stem of Rauwolfia serpentina was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) method. Phytochemical analysis revealed the presence of phenols and flavonoids. The total flavonoids and alkaloids content of Rauwolfia serpentina stem of hydroalcoholic extract was 1.086 and 2.364mg/100mg respectively. Ascorbic acid used as standards was also evaluated for comparison. The extract showed dose dependent free radical scavenging property in the tested models. Rauwolfia serpentina stem extract showed IC50 value 68.10?g/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=17.68?g/ml). The present study describes the phytochemical profile and antioxidant activity of Rauwolfia serpentina which will further used for medicinal applications.

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Qualitative Aspects of Some Traditional Landraces of the Tomato “Piennolo” (Solanum lycopersicum L.) of the Campania Region, Southern Italy

Antioxidants ◽

10.3390/antiox9070565 ◽

2020 ◽

Vol 9 (7) ◽

pp. 565 ◽

Cited By ~ 1

Author(s):

Florinda Fratianni ◽

Autilia Cozzolino ◽

Antonio d’Acierno ◽

Filomena Nazzaro ◽

Riccardo Riccardi ◽

...

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Total Polyphenols ◽

Campania Region ◽

Solanum Lycopersicum L ◽

Discrete Amount ◽

Fresh Products ◽

Brix Value ◽

In Vitro Antioxidant Activity

Our study aimed to analyze some qualitative aspects of five landraces of the tomato “Piennolo,” typical of the Vesuvian area, Italy, and determine the in vitro antioxidant activity. All samples showed a high °Brix value and acidity, as well as a discrete amount of reducing sugars, indicating their good quality as fresh products. They showed a high content of lycopene (up to 218 μg g−1 of fresh product) and ascorbic acid (up to 238 μg g−1 of fresh product). The content of total polyphenols was never less than 278 μg g−1 of fresh product. Hyperoside, chlorogenic and gallic acids were the most abundant polyphenols. The interrelationships between the parameters analyzed and the different landraces showed that total polyphenols could have mostly affected (ρ = 0.76) the antioxidant activity more than lycopene (ρ = −0.96). The interrelationships between the most abundant polyphenols and antioxidant activity showed that hyperoside, although the most abundant, negatively affected (ρ = −0.93) the antioxidant activity. Due to the high content of lycopene, ascorbic acid, and hyperoside, the five landraces of the tomato “Piennolo” could be considered promising in terms of their potential healthy characteristics.

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A Comparative Study of Antioxidant Activity between Ethanolic and Aqueous Extract of Cardiospermum halicacabum

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00820 ◽

2021 ◽

pp. 4715-4718

Author(s):

Kumudhavalli M.V. ◽

Nandhinipriya V.

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Hydroxyl Radical ◽

Aqueous Extract ◽

Research Work ◽

Assay Method ◽

Vitro Assay ◽

Aqueous Solvent ◽

Dpph Method

Cardiospermum halicacabum is conventionally used in the treatment of stiffness of limbs, chronic bronchitis, and snakebites. The whole plant of Cardiospermum halicacabum has been extracted using ethanol and aqueous solvent and estimation of their antioxidant activity done for predicted current research work. Both ethanol and aqueous extracts illustrate important antioxidant activity in the DPPH method, nitric oxide method, and hydroxyl radical-induced In-vitro assay method. The DPPH radical inhibition (%) was 53.13, 57.13, and 79.12 for EECH, AECH, and Standard ascorbic acid respectively. The Cardiospermum halicacabum extracts (EECH and AECH) showed significant free radical scavenging action against nitric oxide (NO) induced release of free radicals at the concentrations 250μg/ml, showing 29.22% and 63.32% of nitric oxide inhibition, respectively. The EECH and AECH extracts (25-400μg/ml) significantly scavenged the hydroxyl radical generated by the EDTA/HO system, when compared to that of ascorbic acid. The plant Cardiospermum halicacabum has been used to treat different ailments. The present study clearly showed that both the extracts viz. EECH and AECH of the plant possess significant antioxidant activity. Moreover, ethanolic extract was proved as more potent candidature as compared to aqueous extract. The molecular level mechanism is the future interest of the researchers to prove the traditional use of the plant.

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