scholarly journals Morphological changes of rat jejunum after whole body γ-irradiation and their impact in biodosimetry

2008 ◽  
pp. 475-479
Author(s):  
D Driák ◽  
J Österreicher ◽  
J Vávrová ◽  
Z Řeháková ◽  
Z Vilasová
Keyword(s):  
Morphological Changes ◽  
Absorbed Dose ◽  
Whole Body ◽  
Control Group ◽  
Specific Marker ◽  
Rat Jejunum ◽  
Γ Irradiation ◽  
Experimental Conditions ◽  
The North ◽  
Causal Therapy

Gastrointestinal form is the second stage of the Acute Radiation Syndrome (ARS) with a threshold dose of 8 Gy. It represents an absolutely lethal clinical-pathological unit, enteritis necrohemorrhagica (duodenitis, jejunitis, ileitis, respectively) with unknown causal therapy. The purpose of our study has been to evaluate the morphological changes in a model of radiationinduced enteritis in rats and estimate the significance of changes in biodosimetry. Wistar rats were randomly divided into 21 groups, 10 animals per group. Samples of the jejunum were taken 24, 48, 72, and 96 h after the whole-body γ-irradiation with the doses of 1, 5, 10, 15, and 20 Gy, and routinely stained with hematoxylin and eosin. Five morphometric markers – intercryptal distance, enterocytal height on the top and base of villus, length of basal lamina of 10 enterocytes and enterocytal width – in irradiated rat jejunum were examined. The results were compared with sham-irradiated control group. After lethal doses of irradiation, all morphometric parameters of jejunum significantly changed. With the exception of intercryptal distance, they might be considered as suitable biodosimetric markers under these experimental conditions. Our morphometry results in radiation-induced jejunitis are in accordance with those in other studies. We were the first who quantified morphological postirradiation changes in animal jejunum. Some of them might be used under experimental conditions. This experimental study is a predecessor of the clinical assessment of a specific marker. Under clinical practice, the sensitive biodosimetric parameter could serve as one of the guidance for evaluation of the absorbed dose in irradiated troops as well as rescue workers. This is in accordance with tasks and Standardization Agreement of the North Atlantic Treaty Organization.

scholarly journals Expression of phospho-Elk-1 in rat gut after the whole body γ-irradiation

2008 ◽  
pp. 753-760
Author(s):  
D Driák ◽  
J Österreicher ◽  
Z Řeháková ◽  
Z Vilasová ◽  
J Vávrová
Keyword(s):  
Whole Body ◽  
Rat Jejunum ◽  
Γ Irradiation ◽  
Time Intervals ◽  
Laboratory Rats ◽  
Second Stage ◽  
Causal Therapy ◽  
Radiation Induced ◽  
Sublethal Doses ◽  
Lethal Doses

Gastrointestinal form is the second stage of acute radiation syndrome (ARS) with a threshold dose of 8 Gy in man. It represents an absolutely lethal clinical-pathological unit, necrohemorrhagic enteritis and proctocolitis, with unknown causal therapy. Elk-1 is a protein acting as a transcription factor activating specified genes. The purpose of our study was to examine the expression of phospho-Elk-1 in irradiated jejunum and transversal colon of rats with radiation-induced enterocolitis and to assess the importance of this transcriptional factor as a biodosimetric marker of radiation-induced enteropathy. The laboratory rats were randomly divided into 21 groups, 10 animals per group, and irradiated with whole body γ-irradiation of 1, 5, 10, 15, and 20 Gy. Samples of jejunum and transversal colon were taken 24, 48, 72, and 96 hours later, immunohistochemically stained, and the phospho-Elk-1 expression was examined using computer image analysis. A group of 10 shamirradiated animals was used as control. Significantly increased expression of phospho-Elk-1 in rat jejunum has been found in all time intervals after irradiation by sublethal doses of 1 and 5 Gy, whereas after the irradiation by lethal doses, the expression of phospho-Elk-1 in rat jejunum varied considerably. Significantly increased expression of phospho-Elk-1 in transversal colon has also been found in the first days after irradiation by sublethal doses of 1 and 5 Gy. After irradiation by lethal doses, tere was no uniform pattern of the changes in the expression of phospho-Elk1 in rat transversal colon. The detection of phospho-Elk-1 might be considered as a suitable and very sensitive biodosimetric marker of radiation-induced injury of small and large intestine. According to our knowledge, this is the first study on the phospho-Elk-1 expression in irradiated jejunum and transversal colon in the rat.

scholarly journals Hepatic Gene Expression Changes in Rats Internally Exposed to Radioactive 56MnO2 Particles at Low Doses

2021 ◽  
Vol 43 (2) ◽  
pp. 758-766
Author(s):  
Bakhyt Ruslanova ◽  
Zhaslan Abishev ◽  
Nailya Chaizhunussova ◽  
Dariya Shabdarbayeva ◽  
Sholpan Tokesheva ◽  
...  
Keyword(s):  
Atomic Bomb ◽  
Kinase Inhibitor ◽  
Biological Effects ◽  
Nuclear Factor Κb ◽  
Internal Exposure ◽  
Whole Body ◽  
Control Group ◽  
Gene Expressions ◽  
Γ Irradiation ◽  
Γ Rays

We have studied the biological effects of the internal exposure to radioactive manganese-56 dioxide (56MnO2), the major radioisotope dust found in soil after atomic bomb explosions. Our previous study of blood chemistry indicated a possible adverse effect of 56MnO2 on the liver. In the present study, we further examined the effects on the liver by determining changes in hepatic gene expressions. Male Wistar rats were exposed to 56MnO2 particles (three groups with the whole-body doses of 41, 90, and 100 mGy), stable MnO2 particles, or external 60Co γ-rays (2 Gy), and were examined together with the non-treated control group on postexposure day 3 and day 61. No histopathological changes were observed in the liver. The mRNA expression of a p53-related gene, the cyclin-dependent kinase inhibitor 1A, increased in 56MnO2 as well as in γ-ray irradiated groups on postexposure day 3 and day 61. The expression of a stress-responsive gene, nuclear factor κB, was also increased by 56MnO2 and γ-rays on postexposure day 3. However, the expression of cytokine genes (interleukin-6 or chemokine ligand 2) or fibrosis-related TGF-β/Smad genes (Tgfb1, Smad3, or Smad4) was not altered by the exposure. Our data demonstrated that the internal exposure to 56MnO2 particles at less than 0.1 Gy significantly affected the short-term gene expressions in the liver in a similar manner with 2 Gy of external γ-irradiation. These changes may be adaptive responses because no changes occurred in cytokine or TGF-β/Smad gene expressions.

scholarly journals Enterosgel role in neurodegenerative changes in the retina rats under the influence of Cr (VI) - induced retinopathy by study morphological changes in dynamic

2021 ◽  
pp. 14-20
Author(s):  
Olena Kuzenko ◽  
Yevhen Kuzenko ◽  
Yuri Demin
Keyword(s):  
Drinking Water ◽  
Morphological Changes ◽  
White Male ◽  
Degenerative Changes ◽  
Male Rats ◽  
Control Group ◽  
Fiber Layer ◽  
Experimental Conditions ◽  
Typical Structure ◽  
Ganglion Neurons

Chromium galvanic production have leaded to biosphere pollution. Therefore advisable to study of role in the neurodegenerative development in retinal diseases under experimental conditions. The aim is to study the Enterosgel effect on morphological changes in rats retina with Cr(VI) – induced retinopathy. Materials and methods. An experimental study had carried out on 72 outbred white male rats. The rats had divided into 3 groups: I – control group of intact rats (n = 24). Control rats were received drinking water, II group – rats (n = 24), were received drinking water with Cr (VI) (K2Cr2O7) – 0.02 mol/L, III group – animals (n = 24) were received drinking water with K2Cr2O7– 0.02 mol/L and hydrogel methylsilicic acid (Enterosgel) at a dose of 0.8 mg/kg per day as a corrector. The animals had been decapitated under ether anesthesia. The retina had been studied on days 20, 40 and 60 of the experiment. Morphologically and morphometrically they had analyzed. Results. According to histological studies, it has proved that Cr (VI) causes dystrophic and degenerative changes in all rats retina layers. They increase as the duration of the experiment. The use of Enterosgel as a corrective therapy showed positive results in restoring the morphological structure of rats retina. After Enterosgel 20 days using as a corrector of Cr (VI) exposure, there is a barely noticeable swelling of the outer and inner nuclear layers. Other layers of the retina, morphologically, look undamaged. Forty days Enterosgel treatment have outer and inner nuclear layer edema of retina of animals persists but does not increase. It is easy noticeable swelling of the outer and inner layers of mesh, but no signs of damage processes of cell populations nuclear layers. State ganglionic layer and nerve fiber layer entirely satisfactory. These pathological changes are not critical. After 60 days from the beginning of loading of Cr (VI) and application of Enterosgel in the retina of rats there are initial degenerative changes in the photosensory layer. Cystic dilated outer segments of rods and cones were visible throughout, and areas of their fragmentation were observed. Ganglion neurons are not damaged, but their axons appear somewhat thickened and fluffy. But in general, the typical structure of the retina is preserved. Conclusions. Chromium-induced toxicity in rats is characterized by pronounced histological and morphometric changes and retinal thickness, which appear after 20 days, increase by 40 days and acquire maximum transformations after 60 days of the experiment. The use of Enterosgel improves picture morphological structures of the retina in rats under the influence of Cr (VI). The changes were expressed on days 20 and 40, which indicates the presence of protective properties for the retina

scholarly journals Evaluation of radioprotective effect of carnosine (beta- alanyl-1- histidine) on the wound healing in rats

2005 ◽  
Vol 13 (3) ◽  
pp. 253-258 ◽  
Author(s):  
Rosana Aramaki Tanaka ◽  
Flávia Maria de Moraes Ramos ◽  
Solange Maria de Almeida ◽  
Mário Roberto Vizioli ◽  
Frab Norberto Bóscolo
Keyword(s):  
Wound Healing ◽  
Surgical Procedure ◽  
Tissue Repair ◽  
Radioprotective Effect ◽  
Male Rats ◽  
Whole Body ◽  
Control Group ◽  
Experimental Conditions ◽  
Anterior Dorsal ◽  
Radioprotective Substance

The purpose of this study was to evaluate the radioprotective effect of carnosine (beta- alanyl-1-histidine) on the wound healing in rats. Therefore, 48 male rats were submitted to a surgical procedure to perform a rectangular wound in the anterior-dorsal region. The animals were divided into 4 experimental groups randomly chosen: control; irradiated; carnosine irradiated and carnosine group. The irradiated and carnosine irradiated group were exposed to a dose (6Gy) of gamma irradiation, in the whole body, 72 hours after surgery. The carnosine and carnosine irradiated groups, in addition to the surgical procedure and the irradiation, received two doses of carnosine aqueous solution, the first one being injected 48 hours after surgery, and the second one 1 hour and 30 minutes before irradiation. The tissue repair of the 4 groups was evaluated at 4, 7, 14, and 21 days after inflicting the wound, by morphological, histochemical and histophysical methods. At all examined periods, it could be observed that the animals from the carnosine irradiated group presented a better developed granulation tissue than the irradiated group and closely similar to that of the control group. Thus, under the experimental conditions used, it was possible to conclude that carnosine is an effective radioprotective substance.

Effects of Ganoderma Lucidum and Krestin on Subset T-cell in Spleen of γ-irradiated Mice

1995 ◽  
Vol 23 (03n04) ◽  
pp. 289-298 ◽  
Author(s):  
Wang-Chi Chen ◽  
Dou-Mong Hau ◽  
Chih-Chung Wang ◽  
I-Hsin Lin ◽  
Shiuh-Sheng Lee
Keyword(s):  
Body Weight ◽  
Ganoderma Lucidum ◽  
Radiation Treatment ◽  
Whole Body ◽  
Control Group ◽  
Γ Irradiation ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Better Than

Effects of Ganoderma lucidum (Gl) and Krestin (PSK) extracts on spleen, thymus and splenocytes in γ-irradiated mice were investigated in this study. ICR strain male mice were divided into five groups. Group A was the normal control. Group B, the experimental control, was treated with Gl. Group C, the radiation treatment control, was treated with whole body exposure to 4 Gy γ-irradiation (RT). Group D was treated with RT and Gl. Group E was treated with RT and PSK. The dosage of Gl was 400 mg/day/kg body weight and PSK was 500 mg/day/kg body weight. Our results indicated that the relative thymus weight in groups D and E were higher than group C on day 28 after γ-irradiation. Group D was the highest in all the experimental groups. CD4 and CD8 splenocytes in group D were higher than group C on days 7 and 28. Gl was better than PSK in repairing the damage of subset T-cells in the spleen of γ-irradiated mice.

EFFECTS OF 900 MHZ RADIOFREQUENCY RADIATION ON THE RATS’ LIVER

2016 ◽  
Vol 78 (6-7) ◽  
Author(s):  
Usikalu M. R. ◽  
Rotimi S. O. ◽  
Achuka J. A.
Keyword(s):  
Liver Function ◽  
Mobile Phone ◽  
Liver Function Tests ◽  
Male Rats ◽  
Whole Body ◽  
Control Group ◽  
Experimental Conditions ◽  
Test Kit ◽  
Group B ◽  
Mobile Phone Radiation

The goal of the study is to evaluate the liver function tests and histological alterations of the rats liver cells after exposure to mobile phone radiation. To perform the liver function test Aspartate transaminase (AST) U.V kinetic test kit produced by CYPRESS DIAGNOSTICS and spectrophotometer was used. Wistar rats (male, 5 week old, approximate body weight 30-40 g) (N = 12) were exposed to 900 MHz mobile phone radiation of Global System Mobile signal modulation (GSM), whole body average specific absorption rate SAR of 1.090W/kg. Twelve (12) male rats were used for the study divided into 3 groups of 4 rats each. Group B and Group C were exposed to 4 hours/day and 8 hours/day mobile phone radiation during calling mode for 2 months respectively while Group A served as control.  There was significant reduction in total  protein, globulin,  while there was significantly increased in Glutamate Oxalacetate Transaminase (GOT), Alkaline Phosphatase (ALP), Albumin, total bilirubin in the rats (Group B and C) exposed to mobile phone radiation when compared to the control group but there was no histological alteration in the arrangement of cells in both control and exposed group. The results of this study suggest that, under the experimental conditions applied, repeated 900 MHz irradiation could modify liver functions.

Effect of Gamma-Irradiation on Prenatal Development of the Tongue in CD-1 Mice

1994 ◽  
Vol 31 (4) ◽  
pp. 246-249
Author(s):  
A. Yousef Saad ◽  
Ali A. Abdelazim ◽  
Mostafa M. El-Khashab ◽  
Mohamed A. Mansour
Keyword(s):  
Adverse Effect ◽  
Microscopic Examination ◽  
Prenatal Development ◽  
Whole Body ◽  
Control Group ◽  
Control Groups ◽  
Γ Irradiation ◽  
Hematoxylin And Eosin ◽  
Mouse Fetuses ◽  
And Control

Development of the tongue in murine fetuses, in which the dams were exposed to whole body γ-Irradiation (400 rads) on the 12th day postcoitum, was studied and compared with unirradiated controls. Experimental and control groups were killed on day 18 of gestation and the fetuses were removed via laparotomy. The fetal heads were excised, fixed in Bouin's solution, prepared for paraffin sectioning, and stained with hematoxylin and eosin for light microscopic examination. Histologic examination of serial coronal sections demonstrated that all of the experimental fetuses possessed malformed, poorly developed tongues, when compared to those of the control group. It was concluded that γ-irradiation has an adverse effect on tongue development in mouse fetuses.

Effects of Ganoderma Lucidum and Krestin on Cellular Immunocompetence in γ-Ray-irradiated Mice

1995 ◽  
Vol 23 (01) ◽  
pp. 71-80 ◽  
Author(s):  
Wang-Chi Chen ◽  
Dou-Mong Hau ◽  
Shiuh-Sheng Lee
Keyword(s):  
Body Weight ◽  
Ganoderma Lucidum ◽  
Radiation Treatment ◽  
Whole Body ◽  
Control Group ◽  
Con A ◽  
Γ Irradiation ◽  
Leukocyte Counts ◽  
Γ Ray ◽  
Group D

The effects of Ganoderma lucidum (Gl) and Krestin (PSK) extracts on cellular immunocompetence, leukocyte counts and differential count in γ-irradiated mice were investigated in this study. ICR strain male mice were used and randomly divided into five groups. Group A is normal control. Group B, the experimental control, was treated with Gl. Group C, the radiation treatment control, was treated with whole body exposure to 4 Gy γ-irradiation (RT). Group D was treated with RT and Gl. Group E was treated with RT and PSK. The dosage of Gl was 400 mg/day/kg body weight and PSK was 500 mg/day/kg body weight. After irradiation, six mice from each group were sacrificed on day 7 and the other six on day 28. Cellular immunocompetence was measured by means of 3 H -thymidine incorporation with splenic cells stimulated through mitogens such as PHA, Con A and LPS. The results revealed that relative splenic weight in Groups D and E were higher than group C on day 28 after γ-irradiation, Group D was the highest in all the experimental groups. Leukocyte counts were decreased significantly in Groups D and E on day 7, the former was a little higher than the latter. Gl administration showed an increase in the leukocyte count in Group D on day 28. The blastogenic response of splenocytes to PHA and Con A in groups D and E were higher than in Group C on days 7 and 28. We suggested that Gl and PSK were effective in enhancing the recovery of cellular immunocompetence from γ-ray irradiation.

scholarly journals Assessment Of Some Functional Indicators Of The Oral Cavity Working In Tobacco Production

2020 ◽  
Vol 02 (10) ◽  
pp. 108-112
Author(s):  
Nazarova Nodira Sharipovna ◽  
◽  
Sitora Baizakova ◽  
Sevinch Ismailova ◽  
◽  
...  
Keyword(s):  
Oral Cavity ◽  
Morphological Changes ◽  
Subcutaneous Tissue ◽  
Allergic Diseases ◽  
Control Group ◽  
Experimental Conditions ◽  
Prophylactic Measures ◽  
Tobacco Extract ◽  
Simplex Virus ◽  
The Impact

To date, it has been established that 70% of people involved in tobacco growing have various diseases [4,6,]. Comparison of morbidity rates with temporary disability of workers employed in tobacco growing and the control group shows that tobacco growers get sick more often than others. The number of diseases they have is 37%, and the number of days of disability is 30% higher than in other types of agricultural work [8,4,1]. Diseases of the respiratory and gastrointestinal tract, infectious and allergic diseases, nervous and cardiovascular systems, skin and subcutaneous tissue occupy the leading place in the structure of tobacco growers' morbidity. However, there is no information on the impact of unfavorable factors of the tobacco-growing industry on the organs and tissues of the oral cavity of workers and the corresponding therapeutic and prophylactic measures, with the exception of individual works [7,2] in the form of an article, where long-term exposure to tobacco extract under experimental conditions, combined with a single dose and a population herpes simplex virus, cause clinical and morphological changes resembling leukoplakia.

scholarly journals Effect of Low-Dose Gamma Radiation and Lipoic Acid on High- Radiation-Dose Induced Rat Brain Injuries

Dose-Response ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 155932582110448
Author(s):  
Nahed Abdel-Aziz ◽  
Ahmed A Elkady ◽  
Eman M Elgazzar
Keyword(s):  
Brain Injury ◽  
Lipoic Acid ◽  
Low Dose ◽  
Brain Injuries ◽  
Neuroprotective Activity ◽  
Whole Body ◽  
High Dose ◽  
Γ Irradiation ◽  
Experimental Conditions ◽  
Adaptive Mechanisms

Aim This work aims to investigate the possible radio-adaptive mechanisms induced by low-dose (LD) whole-body γ-irradiation alone or combined with alpha-lipoic acid (ALA) administration in modulating high-dose (HD) head irradiation–induced brain injury in rats. Materials and Methods Rats were irradiated with LD (.25 Gy) 24 hours prior HD (20 Gy), and subjected to ALA (100 mg/kg/day) 5 minutes after HD and continued for 10 days. At the end of the experiment, animals were sacrificed and brain samples were dissected for biochemical and histopathological examinations. Results HD irradiation-induced brain injury as manifested by elevation of oxidative stress, DNA damage, apoptotic, and inflammatory markers in brain tissue. Histological examination of brain sections showed marked alterations. However, LD alone or combined with ALA ameliorated the changes induced by HD. Conclusion Under the present experimental conditions, LD whole-body irradiation exhibited neuroprotective activity against detrimental effects of a subsequent HD head irradiation. This effect might be due to the adaptive response induced by LD that activated the anti-oxidative, anti-apoptotic, and anti-inflammatory mechanisms in the affected animals making them able to cope with the subsequent high-dose exposure. However, the combined LD exposure and ALA supplementation produced a further modulating effect in the HD-irradiated rats.

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